FvH4_1g04210
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008072
Physical Location & Seq
Reverse (-)
2222754 .. 2230430
7677 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04210.t7

Sequence Viewer

Length: 4263 bp
ATGTGGAACTCGGCGGAGAATGCGTTTGCGAGGACGGCGTCGTTTAGGGAGGAGGGGGAGGACGAGGAGGCTTTACGGTGGGCGGCGTTGGAGAGGCTGCCGACATACGCCAGAGTCCGACGTGGCATTTTTAAGAACATTGTGGGAGATACCAAGGAGGTTGACGTCAGTGAGCTCCAGGCTCAGGAGCAGAAGCTCTTGCTTGATCGGCTAGTTAACTCGGCCGAACAAGACCCCGAGCAGTTCTTTCGGAAAATGCGACTCCGGTTCAATGCAGTTGATTTGGACTTTCCAAAGATCGAGGTGCGGTTTCAGGATTTGAAGGTTGAAGCTTTTGTTCATGTGGGAAGCAGGGCACTGCCGACTATTCCGAATTTCGTTTTCAATATGACCGAGGCGCTTCTGAGGCAATTACGGTTATTGAGGAGCAAGAGAAGTAAATTAACGATTTTAGACAACATTAGTGGGATTATAAGACCTTCAAGATTGACGTTGCTATTGGGCCCACCGAGCTCCGGCAAGACAACGCTGCTATTGGCTCTCGCTGGACGCCTTGGAACTGGTCTTCAGATGTCAGGTAAAACCACATACAATGGCCATGGATTGAATGAGTTTGTGCCGCAGAGGACGGCAGCCTATGTCAGCCAACAGGACTGGTTTGCGGCCGAGATGACTGTGAGGGAGACTCTTGACTTTGCAGGACGGTGTCAAGGTGTTGGATTCAAATATGATATGCTAGTAGAACTTGCAAGAAGAGAAAAGATTGCAGGGATAAAACCCGATGGTGATCTTGATATATTCATGAAGTCATTAGCTCTTGGTGAGAAAGAGACTAGCCTTGTGGTAGAGTACATCATGAAGATTTTAGGGTTGGACATATGTGCTGATACGTTGGTAGGAGATGAAATGCTCAAGGGTATATCAGGGGGCCAAAAGAAGCGGCTTACTTCAGGTGAATTACTAGTAGGTCCAGCAAGAGTGTTGTTCATGGATGAAATATCAACTGGGCTTGACAGTTCCACTACTTATCAAATCATCAAGTATCTTAGGCATTCAACTCATGCACTTGATTCCACCACTATTATATCTTTACTTCAACCTGCTCCAGAGACCTACGAGTTGTTCGATGATGTTATACTTTTGTGTGAGGGTCAGATCGTGTATCAGGGACCCCGGCAAGCTGCTCTTGATTTCTTTTCATACATGGGGTTTAGTTGTCCCCAGCGAAAGAATGTGGCAGACTTCTTGCAAGAAGTTATATCAAAGAAGGATCAAGAGCAGTACTGGTCCAATCCTGATCTTCCATACCGTTACATACCACCAGCAAAGTTTGTTGAAGCTTTTCCTTCATTTCAAGATGGGAAGAATTTGTCAGAAGAGTTGAAAGTTCCTTTTGATAAACGCTATAACCACCCTGCAGCTTTGGCAACTTCTCTCTATGGCATGAGGAGGATGGAACTTCTCAAGACAAGCTTTAACTGGCAAGTGCTACTGATGAAGCGAAATGCATTTATTTATATATTTAAATTCGTTCAGCTTCTTTTTGTTGCTTTGGTCACAATGAGTGTTTTCTGCCGGACAAAAATGCACCATGATACAATTGATGATGCAGCCTTGTATCTCGGGGCACTATACTTTTCCATGGTTATTATTCTTTTTAATGGCTTTATGGAGGTGCCAATGTTGGTTGCCAAGCTTCCAGTGCTTTACAAGCATAGGGACTTGCACTTCTATCCAAGTTGGGTCTATACTCTTCCTTCCTGGCTCTTGAGTATTCCAAATTCACTCATAGAGTCTGGATTTTGGGTGGCAATCACATACTATGTCATTGGATTTGATCCTGCAATTTCTAGATTTTTTGGGCAGTTCTTGGTATATTTCCTTCTGCACCAGATGTCCACAGCTCTCTTCCGTTTTATGGGATCCTTGGGTCGAAATATGATAGTTGCCAACACTTTTGGATCTTTTGCCATGTTGATTGTAATGGCTCTTGGAGGGTACATCATTTCTAGAGATCGTATACCAAAATGGTGGATTTGGGGTTTCTGGTTTTCTCCTTTGATGTATGCTCAAAATGCAGCTTCTGTAAACGAATTCCTCGGACACTCCTGGAATAAGGGACATGAGTATGAGACCGGTCTTTCATTAGGTCAGTCATTACTAAAAGCACGCAGTTTGTTTGCGGAGAGATACTGGTTTTGGATTGGTATTGGTGCTTTGCTTGGATATACAGTTTTATTCAATTTGCTATTCACATTCTTCCTTGCTTACCTGAACCCCTTGGGGAAGCAACAAGTTGTAGTGTCGAAGGAAGAGCTAGAAGAGAGAGAAAGGAGAAGAACAGGAGAAAATGTTGTTATTGAGCTGAGACAGTACTTGAAGCATTCAGAGTCACTTAATGGAAAGTACTTTAAGCAGAGAGGAATGGTGCTCCCATTTCAACCACTTTCCATGTCTTTCAGCAATATCAATTACTATGTCGATATCCCTCTGGAATTGAAGCAACAGGGAATACAAGAAGAGAGATTGCAGCTGTTGGTTGATGTAACTGGAGCGTTTAGGCCCGGGGTGCTTACAGCATTGGTTGGAGTAAGTGGTGCTGGCAAAACCACCCTTATGGACGTGTTAGCTGGCAGAAAAACTGGTGGGATCATAGAAGGGAGCATTAATATTTCTGGTTATCCAAAAAAGCAAGAAACTTTCGCTAGAATTTCCGGTTACTGTGAGCAATCTGATATTCACTCCCCCTGCTTGACTGTCGTTGAATCACTTATGTTCTCTAGTTGGCTTCGCTTGCCATCAGAAGTTGACTTGGATACGCAGAAGGCTTTTGTAGAGGAGGTGATGGAACTTGTGGAGCTCACTCCATTAAGAGGGGCACTGGTTGGTTTACCGGGGGTGAATGGCTTGTCCACAGAACAACGAAAAAGGCTAACTATAGCTGTGGAGCTGGTTGCTAATCCATCTATAGTATTTATGGATGAACCCACATCTGGGCTAGATGCAAGGTCTGCAGCCATTGTGATGAGGACTGTGAGGAATATAGTCAATACTGGACGTACTATAGTGTGCACAATCCACCAGCCTAGCATAGACATTTTTGAATCCTTTGATGAGCTTCTGTTTCTGAAGCGCGGAGGTCAGCTCATATATGCTGGTCCACTTGGACCCAGGTCTTCTGAACTCATCAAGTACTTTGAGGCGATTGAAGGAGTACAAAAGATCAGGCCTGGATATAATCCTGCTGCATGGATGCTGGATGTCACTTCTCCAACTGAAGAAAGCCGTCTGGGGGTGGATTTCGCAGAAATTTACAGAAGTTCAAACTTGTTTCAACGTAACATAGACTTGGTTGAACACCTCAGCAAGCCAAGTGCTAATTCCAAAGAATTGAACTTTCCAACCAAGTACTCTCAGACATCATTTGAACAATTTCTCACTTGTCTATGGAAGCAAAATCTGTCTTATTGGCGGAACCCTCAGTACACCGCAGTTCGGTTCTTTTACACAGTCATCATCTCCTTGATGTTAGGAACAATTTGTTGGAGATTTGGGGCAAAGAGGGAGACTCAGCAGGACTTACTAAATGCCATGGGATCCCTATATGCAGCAATCCTATTCAGTGGAATCACCAATGCCACTGCTGTTCAACCTGTTGTTTCTATAGAAAGATTTGTCTCATATCGAGAGAGAGCTGCTGGGATGTACTCGGCATTACCATTTGCATTTGCTCAGGTTGCGATAGAGTTCCCTTATGTGTTTGCGCAAGCAGTCATCTACTGCACAATTTTCTACTCCACGGCTGCATTTGACTGGACACTCTTGAAGTTTGTTTGGTACTTATTCTTCATGTATTTCACAATGCTGTACTTCACCTTATACGGAATGATGACAACAGCCGTCACACCAAATCATAACGTAGCTTCCATAATAGCTGCACCATTTTACATGCTATGGAATCTTTTCAGTGGCTTCATGATTCCTCACAAGAGAATCCCAATGTGGTGGAGATGGTATTACTGGGCAAACCCTGTAGCCTGGAGTCTGTATGGCCTGATTGTTTCGCAGTACGGTGACGATGACAGTCTTGTGAAGCTAGCAGATGGAGAAACAACAATTTCGATAAGGCTGGTACTCAAAGTTGGTCTCGGATACAGGCATGATTTCCTCGGTGTAGCCGGAGTTATGGTCGTCGGTTTCTGCATTCTTTTCGCCATAATTTTTGCCTATGCCATAAAAGCATTCAACTTCCAGAGGAGATGA

Protein Analysis

1421

Amino Acids

161.63

Weight (kDa)

8.46

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 73 - 126 1.3e-07 ABC-transporter N-terminal
ABC_tran PF00005 151 - 332 4.2e-14 ABC transporter
ABC2_membrane_7 PF19055 366 - 439 3.7e-06 ABC-2 type transporter
ABC2_membrane PF01061 488 - 692 2.5e-36 ABC-2 type transporter
PDR_assoc PF08370 731 - 795 7.5e-25 Plant PDR ABC transporter associated
ABC_tran PF00005 875 - 1025 2.2e-18 ABC transporter
ABC2_membrane PF01061 1170 - 1384 4e-55 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
AatII GACGTC 1 cut(s) 168
Acc16I TGCGCA 1 cut(s) 3765
Acc36I ACCTGC 1 cut(s) 1108
AccB1I GGYRCC 1 cut(s) 1675
AccI GTMKAC 1 cut(s) 2020
AccII CGCG 1 cut(s) 3135
AclWI GGATC 8 cut(s) 1278, 1832, 1917, 1930, 1969, 2657, 3591, 3604
AcoI YGGCCR 3 cut(s) 222, 595, 663
AcsI RAATTY 7 cut(s) 373, 1366, 1526, 1780, 2093, 2709, 3309
AcuI CTGAAG 4 cut(s) 551, 933, 3149, 3297
AcyI GRCGYC 3 cut(s) 38, 165, 550
AfiI CCNNNNNNNGG 8 cut(s) 184, 515, 1918, 2873, 2993, 2994, 3292, 3495
AflIII ACRYGT 1 cut(s) 2622
AgeI ACCGGT 1 cut(s) 2135
AhlI ACTAGT 1 cut(s) 961
AjiI CACGTC 2 cut(s) 122, 2623
AjnI CCWGG 6 cut(s) 177, 1760, 2108, 3170, 3229, 4037
AjuI GAANNNNNNNTTGG 4 cut(s) 3364, 3396, 3526, 3558
Alw21I GWGCWC 5 cut(s) 177, 515, 2433, 2862, 3074
Alw26I GTCTC 8 cut(s) 677, 824, 1103, 2126, 2362, 3560, 3682, 4151
Alw44I GTGCAC 1 cut(s) 3070
AlwI GGATC 8 cut(s) 1278, 1832, 1917, 1930, 1969, 2657, 3591, 3604
AlwNI CAGNNNCTG 1 cut(s) 2084
Ama87I CYCGRG 3 cut(s) 236, 1622, 2564
AoxI GGCC 8 cut(s) 222, 502, 595, 663, 928, 2561, 3227, 4051
ApaI GGGCCC 1 cut(s) 506
ApaLI GTGCAC 1 cut(s) 3070
ApoI RAATTY 7 cut(s) 373, 1366, 1526, 1780, 2093, 2709, 3309
AseI ATTAAT 1 cut(s) 2667
AsiGI ACCGGT 1 cut(s) 2135
Asp700I GAANNNNTTC 3 cut(s) 1341, 3432, 3962
AspLEI GCGC 3 cut(s) 400, 3135, 3766
AspS9I GGNCC 9 cut(s) 502, 503, 928, 968, 1169, 1287, 2562, 3158, 3167
AsuC2I CCSGG 4 cut(s) 1174, 2565, 2566, 2895
AsuHPI GGTGA 8 cut(s) 797, 833, 965, 2854, 2911, 3622, 3865, 4085
AsuNHI GCTAGC 1 cut(s) 4096
AvaI CYCGRG 3 cut(s) 236, 1622, 2564
AvaII GGWCC 5 cut(s) 968, 1169, 1287, 3158, 3167
BaeGI GKGCMC 5 cut(s) 358, 506, 1630, 2881, 3074
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BalI TGGCCA 1 cut(s) 597
BamHI GGATCC 2 cut(s) 1922, 3596
BanI GGYRCC 1 cut(s) 1675
BanII GRGCYC 4 cut(s) 177, 506, 515, 2862
BarI GAAGNNNNNNTAC 4 cut(s) 1866, 1898, 3830, 3862
BbsI GAAGAC 2 cut(s) 557, 3168
Bbv12I GWGCWC 5 cut(s) 177, 515, 2433, 2862, 3074
BbvCI CCTCAGC 1 cut(s) 3362
BccI CCATC 8 cut(s) 776, 1352, 1447, 2806, 2839, 2971, 4005, 4097
BceAI ACGGC 5 cut(s) 51, 645, 3270, 3816, 3884
BcgI CGANNNNNNTGC 4 cut(s) 230, 264, 4192, 4226
BciT130I CCWGG 6 cut(s) 179, 1762, 2110, 3172, 3231, 4039
BciVI GTATCC 2 cut(s) 2809, 4145
BcnI CCSGG 4 cut(s) 1174, 2565, 2566, 2895
BcoDI GTCTC 8 cut(s) 677, 824, 1103, 2126, 2362, 3560, 3682, 4151
BcuI ACTAGT 1 cut(s) 961
BfmI CTRYAG 7 cut(s) 1416, 2937, 2967, 3012, 3063, 3663, 4032
BfoI RGCGCY 1 cut(s) 401
BfuAI ACCTGC 1 cut(s) 1108
BfuI GTATCC 2 cut(s) 2809, 4145
BmcAI AGTACT 5 cut(s) 1283, 2375, 2408, 3194, 3410
Bme18I GGWCC 5 cut(s) 968, 1169, 1287, 3158, 3167
BmeT110I CYCGRG 3 cut(s) 236, 1622, 2564
BmgBI CACGTC 2 cut(s) 122, 2623
BmgT120I GGNCC 9 cut(s) 502, 503, 928, 968, 1169, 1287, 2562, 3158, 3167
BmiI GGNNCC 9 cut(s) 504, 929, 1170, 1171, 1677, 1924, 3169, 3476, 3598
BmrI ACTGGG 2 cut(s) 1014, 4030
BmsI GCATC 3 cut(s) 1597, 2992, 3243
BmtI GCTAGC 1 cut(s) 4100
BmuI ACTGGG 2 cut(s) 1014, 4030
BoxI GACNNNNGTC 1 cut(s) 3172
BpiI GAAGAC 2 cut(s) 557, 3168
BplI GAGNNNNNCTC 6 cut(s) 670, 702, 3129, 3161, 3553, 3585
BpmI CTGGAG 4 cut(s) 161, 1089, 2571, 4060
Bpu10I CCTNAGC 3 cut(s) 183, 3362, 3732
BpuEI CTTGAG 3 cut(s) 896, 1448, 1789
BpuMI CCSGG 4 cut(s) 1174, 2565, 2566, 2895
BsaBI GATNNNNATC 1 cut(s) 786
BsaHI GRCGYC 3 cut(s) 38, 165, 550
BsaI GGTCTC 3 cut(s) 1103, 2126, 4151
BsaWI WCCGGW 3 cut(s) 264, 2135, 2714
BsaXI ACNNNNNCTCC 6 cut(s) 8, 38, 2337, 2367, 4173, 4203
Bsc4I CCNNNNNNNGG 8 cut(s) 184, 515, 1918, 2873, 2993, 2994, 3292, 3495
Bse118I RCCGGY 1 cut(s) 2135
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 6 cut(s) 179, 1762, 2110, 3172, 3231, 4039
BseGI GGATG 6 cut(s) 997, 1458, 2986, 3258, 3265, 3708
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 8 cut(s) 184, 515, 1918, 2873, 2993, 2994, 3292, 3495
BseMII CTCAG 8 cut(s) 197, 395, 2357, 3376, 3428, 3494, 3584, 3746
BseRI GAGGAG 5 cut(s) 65, 80, 439, 1462, 2852
BseSI GKGCMC 5 cut(s) 358, 506, 1630, 2881, 3074
BseX3I CGGCCG 2 cut(s) 222, 663
BseYI CCCAGC 2 cut(s) 1221, 3698
BsgI GTGCAG 3 cut(s) 1871, 3766, 3921
Bsh1236I CGCG 1 cut(s) 3135
Bsh1285I CGRYCG 2 cut(s) 225, 666
BshFI GGCC 8 cut(s) 224, 504, 597, 665, 930, 2563, 3229, 4053
BshNI GGYRCC 1 cut(s) 1675
BshTI ACCGGT 1 cut(s) 2135
BsiEI CGRYCG 2 cut(s) 225, 666
BsiHKAI GWGCWC 5 cut(s) 177, 515, 2433, 2862, 3074
BsiHKCI CYCGRG 3 cut(s) 236, 1622, 2564
BsiSI CCGG 9 cut(s) 265, 516, 1174, 1576, 2136, 2565, 2715, 2894, 4179
BslFI GGGAC 4 cut(s) 1182, 1203, 1733, 2133
BslI CCNNNNNNNGG 8 cut(s) 184, 515, 1918, 2873, 2993, 2994, 3292, 3495
BsmAI GTCTC 8 cut(s) 677, 824, 1103, 2126, 2362, 3560, 3682, 4151
BsmFI GGGAC 4 cut(s) 1182, 1203, 1733, 2133
BsmI GAATGC 5 cut(s) 25, 1051, 2383, 4203, 4241
BsnI GGCC 8 cut(s) 224, 504, 597, 665, 930, 2563, 3229, 4053
Bso31I GGTCTC 3 cut(s) 1103, 2126, 4151
BsoBI CYCGRG 3 cut(s) 236, 1622, 2564
Bsp120I GGGCCC 1 cut(s) 502
Bsp1286I GDGCHC 9 cut(s) 177, 358, 506, 515, 1630, 2433, 2862, 2881, 3074
Bsp19I CCATGG 3 cut(s) 598, 1641, 3591
BspANI GGCC 8 cut(s) 224, 504, 597, 665, 930, 2563, 3229, 4053
BspCNI CTCAG 8 cut(s) 196, 396, 2358, 3375, 3427, 3493, 3583, 3745
BspFNI CGCG 1 cut(s) 3135
BspHI TCATGA 3 cut(s) 801, 855, 3975
BspLI GGNNCC 9 cut(s) 504, 929, 1170, 1171, 1677, 1924, 3169, 3476, 3598
BspMAI CTGCAG 2 cut(s) 1420, 3016
BspMI ACCTGC 1 cut(s) 1108
BspOI GCTAGC 1 cut(s) 4100
BspPI GGATC 8 cut(s) 1278, 1832, 1917, 1930, 1969, 2657, 3591, 3604
BspQI GCTCTTC 1 cut(s) 2307
BspT107I GGYRCC 1 cut(s) 1675
BspTNI GGTCTC 3 cut(s) 1103, 2126, 4151
BsrFI RCCGGY 1 cut(s) 2135
BssAI RCCGGY 1 cut(s) 2135
BssNAI GTATAC 1 cut(s) 2021
BssNI GRCGYC 3 cut(s) 38, 165, 550
BssT1I CCWWGG 7 cut(s) 153, 553, 598, 1641, 1926, 2280, 3591
Bst1107I GTATAC 1 cut(s) 2021
Bst2UI CCWGG 6 cut(s) 179, 1762, 2110, 3172, 3231, 4039
Bst6I CTCTTC 7 cut(s) 748, 1371, 1758, 1913, 2307, 2316, 2514
BstACI GRCGYC 3 cut(s) 38, 165, 550
BstAPI GCANNNNNTGC 1 cut(s) 3011
BstC8I GCNNGC 8 cut(s) 1179, 2170, 2602, 2632, 2795, 3368, 3768, 4098
BstDEI CTNAG 9 cut(s) 183, 404, 1046, 2366, 3362, 3414, 3480, 3570, 3732
BstDSI CCRYGG 4 cut(s) 598, 1641, 3591, 3798
BstF5I GGATG 6 cut(s) 997, 1458, 2986, 3258, 3265, 3708
BstFNI CGCG 1 cut(s) 3135
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 3 cut(s) 400, 3135, 3766
BstMAI GTCTC 8 cut(s) 677, 824, 1103, 2126, 2362, 3560, 3682, 4151
BstMCI CGRYCG 2 cut(s) 225, 666
BstNI CCWGG 6 cut(s) 179, 1762, 2110, 3172, 3231, 4039
BstNSI RCATGY 1 cut(s) 3952
BstPAI GACNNNNGTC 1 cut(s) 3172
BstSFI CTRYAG 7 cut(s) 1416, 2937, 2967, 3012, 3063, 3663, 4032
BstSLI GKGCMC 5 cut(s) 358, 506, 1630, 2881, 3074
BstUI CGCG 1 cut(s) 3135
BstV2I GAAGAC 2 cut(s) 557, 3168
BstX2I RGATCY 3 cut(s) 1922, 1961, 3596
BstXI CCANNNNNNTGG 3 cut(s) 2031, 2617, 4005
BstYI RGATCY 3 cut(s) 1922, 1961, 3596
BstZ17I GTATAC 1 cut(s) 2021
BstZI CGGCCG 2 cut(s) 222, 663
BsuI GTATCC 2 cut(s) 2809, 4145
BsuRI GGCC 8 cut(s) 224, 504, 597, 665, 930, 2563, 3229, 4053
BtgI CCRYGG 4 cut(s) 598, 1641, 3591, 3798
BtrI CACGTC 2 cut(s) 122, 2623
BtsCI GGATG 6 cut(s) 997, 1458, 2986, 3258, 3265, 3708
BtsI GCAGTG 2 cut(s) 356, 3639
BtsIMutI CAGTG 7 cut(s) 175, 356, 1707, 2879, 3628, 3639, 3973
BveI ACCTGC 1 cut(s) 1108
Cac8I GCNNGC 8 cut(s) 1179, 2170, 2602, 2632, 2795, 3368, 3768, 4098
CaiI CAGNNNCTG 1 cut(s) 2084
CciI TCATGA 3 cut(s) 801, 855, 3975
CfoI GCGC 3 cut(s) 400, 3135, 3766
Cfr10I RCCGGY 1 cut(s) 2135
Cfr13I GGNCC 9 cut(s) 502, 503, 928, 968, 1169, 1287, 2562, 3158, 3167
Cfr9I CCCGGG 1 cut(s) 2564
CseI GACGC 2 cut(s) 27, 558
CspAI ACCGGT 1 cut(s) 2135
CspCI CAANNNNNGTGG 2 cut(s) 445, 480
DdeI CTNAG 9 cut(s) 183, 404, 1046, 2366, 3362, 3414, 3480, 3570, 3732
DraI TTTAAA 1 cut(s) 1525
EaeI YGGCCR 3 cut(s) 222, 595, 663
EagI CGGCCG 2 cut(s) 222, 663
Eam1104I CTCTTC 7 cut(s) 748, 1371, 1758, 1913, 2307, 2316, 2514
EarI CTCTTC 7 cut(s) 748, 1371, 1758, 1913, 2307, 2316, 2514
EciI GGCGGA 2 cut(s) 29, 3487
Ecl136II GAGCTC 3 cut(s) 175, 513, 2860
EclXI CGGCCG 2 cut(s) 222, 663
Eco130I CCWWGG 7 cut(s) 153, 553, 598, 1641, 1926, 2280, 3591
Eco147I AGGCCT 1 cut(s) 3229
Eco24I GRGCYC 4 cut(s) 177, 506, 515, 2862
Eco31I GGTCTC 3 cut(s) 1103, 2126, 4151
Eco32I GATATC 1 cut(s) 2485
Eco47I GGWCC 5 cut(s) 968, 1169, 1287, 3158, 3167
Eco52I CGGCCG 2 cut(s) 222, 663
Eco53kI GAGCTC 3 cut(s) 175, 513, 2860
Eco57I CTGAAG 4 cut(s) 551, 933, 3149, 3297
Eco88I CYCGRG 3 cut(s) 236, 1622, 2564
EcoICRI GAGCTC 3 cut(s) 175, 513, 2860
EcoO109I RGGNCCY 1 cut(s) 1169
EcoRI GAATTC 1 cut(s) 2093
EcoRII CCWGG 6 cut(s) 177, 1760, 2108, 3170, 3229, 4037
EcoRV GATATC 1 cut(s) 2485
EcoT14I CCWWGG 7 cut(s) 153, 553, 598, 1641, 1926, 2280, 3591
EcoT22I ATGCAT 1 cut(s) 1510
EcoT38I GRGCYC 4 cut(s) 177, 506, 515, 2862
ErhI CCWWGG 7 cut(s) 153, 553, 598, 1641, 1926, 2280, 3591
FalI AAGNNNNNCTT 4 cut(s) 1170, 1202, 1457, 1489
FaqI GGGAC 4 cut(s) 1182, 1203, 1733, 2133
FauNDI CATATG 1 cut(s) 878
FblI GTMKAC 1 cut(s) 2020
FokI GGATG 6 cut(s) 1004, 1465, 2993, 3265, 3272, 3715
FriOI GRGCYC 4 cut(s) 177, 506, 515, 2862
FspI TGCGCA 1 cut(s) 3765
GlaI GCGC 3 cut(s) 399, 3134, 3765
GsaI CCCAGC 2 cut(s) 1225, 3702
GsuI CTGGAG 4 cut(s) 161, 1089, 2571, 4060
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 8 cut(s) 224, 504, 597, 665, 930, 2563, 3229, 4053
HapII CCGG 9 cut(s) 265, 516, 1174, 1576, 2136, 2565, 2715, 2894, 4179
HgaI GACGC 2 cut(s) 27, 558
HhaI GCGC 3 cut(s) 400, 3135, 3766
Hin1I GRCGYC 3 cut(s) 38, 165, 550
Hin6I GCGC 3 cut(s) 398, 3133, 3764
HinP1I GCGC 3 cut(s) 398, 3133, 3764
HincII GTYRAC 3 cut(s) 163, 217, 2809
HindII GTYRAC 3 cut(s) 163, 217, 2809
HindIII AAGCTT 4 cut(s) 330, 1338, 1471, 1694
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 9 cut(s) 265, 516, 1174, 1576, 2136, 2565, 2715, 2894, 4179
HphI GGTGA 8 cut(s) 797, 833, 965, 2854, 2911, 3622, 3865, 4085
Hpy99I CGWCG 3 cut(s) 43, 123, 4196
HpyCH4IV ACGT 8 cut(s) 121, 165, 491, 890, 2622, 3058, 3337, 3918
HpyF3I CTNAG 9 cut(s) 183, 404, 1046, 2366, 3362, 3414, 3480, 3570, 3732
HpySE526I ACGT 8 cut(s) 121, 165, 491, 890, 2622, 3058, 3337, 3918
Hsp92I GRCGYC 3 cut(s) 38, 165, 550
HspAI GCGC 3 cut(s) 398, 3133, 3764
KflI GGGWCCC 1 cut(s) 1169
KspAI GTTAAC 1 cut(s) 217
LguI GCTCTTC 1 cut(s) 2307
LweI GCATC 3 cut(s) 1597, 2992, 3243
MaeII ACGT 8 cut(s) 121, 165, 491, 890, 2622, 3058, 3337, 3918
MaeIII GTNAC 9 cut(s) 1310, 1555, 2391, 2545, 2717, 3262, 3338, 3901, 4073
MfeI CAATTG 1 cut(s) 1599
MflI RGATCY 3 cut(s) 1922, 1961, 3596
MhlI GDGCHC 9 cut(s) 177, 358, 506, 515, 1630, 2433, 2862, 2881, 3074
MlsI TGGCCA 1 cut(s) 597
MluNI TGGCCA 1 cut(s) 597
MlyI GAGTC 7 cut(s) 123, 255, 679, 1802, 2399, 3562, 4051
MmeI TCCRAC 8 cut(s) 69, 142, 697, 852, 2566, 3296, 3425, 3524
Mox20I TGGCCA 1 cut(s) 597
Mph1103I ATGCAT 1 cut(s) 1510
MroXI GAANNNNTTC 3 cut(s) 1341, 3432, 3962
MscI TGGCCA 1 cut(s) 597
MslI CAYNNNNRTG 3 cut(s) 2127, 2615, 3023
Msp20I TGGCCA 1 cut(s) 597
MspA1I CMGCKG 1 cut(s) 2533
MspI CCGG 9 cut(s) 265, 516, 1174, 1576, 2136, 2565, 2715, 2894, 4179
MunI CAATTG 1 cut(s) 1599
Mva1269I GAATGC 5 cut(s) 25, 1051, 2383, 4203, 4241
MvaI CCWGG 6 cut(s) 179, 1762, 2110, 3172, 3231, 4039
MvnI CGCG 1 cut(s) 3135
NciI CCSGG 4 cut(s) 1174, 2565, 2566, 2895
NcoI CCATGG 3 cut(s) 598, 1641, 3591
NdeI CATATG 1 cut(s) 878
NheI GCTAGC 1 cut(s) 4096
NlaIV GGNNCC 9 cut(s) 504, 929, 1170, 1171, 1677, 1924, 3169, 3476, 3598
NmeAIII GCCGAG 3 cut(s) 200, 691, 3689
NmuCI GTSAC 5 cut(s) 1555, 2391, 3262, 3901, 4073
NsbI TGCGCA 1 cut(s) 3765
NsiI ATGCAT 1 cut(s) 1510
NspI RCATGY 1 cut(s) 3952
PagI TCATGA 3 cut(s) 801, 855, 3975
PceI AGGCCT 1 cut(s) 3229
PciSI GCTCTTC 1 cut(s) 2307
PcsI WCGNNNNNNNCGW 2 cut(s) 256, 1122
PctI GAATGC 5 cut(s) 25, 1051, 2383, 4203, 4241
PdmI GAANNNNTTC 3 cut(s) 1341, 3432, 3962
PfeI GAWTC 8 cut(s) 720, 1070, 2765, 3104, 3627, 3958, 3979, 3993
PflFI GACNNNGTC 2 cut(s) 37, 705
PfoI TCCNGGA 1 cut(s) 2108
PinAI ACCGGT 1 cut(s) 2135
PleI GAGTC 7 cut(s) 122, 255, 679, 1801, 2398, 3562, 4050
PpsI GAGTC 7 cut(s) 122, 255, 679, 1801, 2398, 3562, 4050
PpuMI RGGWCCY 1 cut(s) 1169
PshAI GACNNNNGTC 1 cut(s) 3172
PshBI ATTAAT 1 cut(s) 2667
PsiI TTATAA 1 cut(s) 473
Psp124BI GAGCTC 3 cut(s) 177, 515, 2862
Psp5II RGGWCCY 1 cut(s) 1169
Psp6I CCWGG 6 cut(s) 177, 1760, 2108, 3170, 3229, 4037
PspFI CCCAGC 2 cut(s) 1221, 3698
PspGI CCWGG 6 cut(s) 177, 1760, 2108, 3170, 3229, 4037
PspN4I GGNNCC 9 cut(s) 504, 929, 1170, 1171, 1677, 1924, 3169, 3476, 3598
PspOMI GGGCCC 1 cut(s) 502
PspPI GGNCC 9 cut(s) 502, 503, 928, 968, 1169, 1287, 2562, 3158, 3167
PspPPI RGGWCCY 1 cut(s) 1169
PsrI GAACNNNNNNTAC 2 cut(s) 3467, 3499
PstI CTGCAG 2 cut(s) 1420, 3016
PstNI CAGNNNCTG 1 cut(s) 2084
PsuI RGATCY 3 cut(s) 1922, 1961, 3596
PsyI GACNNNGTC 2 cut(s) 37, 705
PvuII CAGCTG 1 cut(s) 2533
RseI CAYNNNNRTG 3 cut(s) 2127, 2615, 3023
SacI GAGCTC 3 cut(s) 177, 515, 2862
SapI GCTCTTC 1 cut(s) 2307
Sau96I GGNCC 9 cut(s) 502, 503, 928, 968, 1169, 1287, 2562, 3158, 3167
ScaI AGTACT 5 cut(s) 1283, 2375, 2408, 3194, 3410
SchI GAGTC 7 cut(s) 123, 255, 679, 1802, 2399, 3562, 4051
SduI GDGCHC 9 cut(s) 177, 358, 506, 515, 1630, 2433, 2862, 2881, 3074
SfaNI GCATC 3 cut(s) 1597, 2992, 3243
SfcI CTRYAG 7 cut(s) 1416, 2937, 2967, 3012, 3063, 3663, 4032
SinI GGWCC 5 cut(s) 968, 1169, 1287, 3158, 3167
SmaI CCCGGG 1 cut(s) 2566
SmiI ATTTAAAT 1 cut(s) 1525
SmiMI CAYNNNNRTG 3 cut(s) 2127, 2615, 3023
SmlI CTYRAG 3 cut(s) 911, 1463, 1768
SmoI CTYRAG 3 cut(s) 911, 1463, 1768
SpeI ACTAGT 1 cut(s) 961
SseBI AGGCCT 1 cut(s) 3229
SspI AATATT 1 cut(s) 2671
SstI GAGCTC 3 cut(s) 177, 515, 2862
StuI AGGCCT 1 cut(s) 3229
StyI CCWWGG 7 cut(s) 153, 553, 598, 1641, 1926, 2280, 3591
SwaI ATTTAAAT 1 cut(s) 1525
TaiI ACGT 8 cut(s) 124, 168, 494, 893, 2625, 3061, 3340, 3921
TaqI TCGA 7 cut(s) 300, 1125, 1933, 2306, 2481, 3685, 4121
TaqII GACCGA 1 cut(s) 407
TauI GCSGC 4 cut(s) 86, 622, 665, 943
TfiI GAWTC 8 cut(s) 720, 1070, 2765, 3104, 3627, 3958, 3979, 3993
TscAI CASTG 7 cut(s) 175, 363, 1707, 2886, 3628, 3646, 3973
TseFI GTSAC 5 cut(s) 1555, 2391, 3262, 3901, 4073
Tsp45I GTSAC 5 cut(s) 1555, 2391, 3262, 3901, 4073
TspGWI ACGGA 2 cut(s) 1901, 3897
TspMI CCCGGG 1 cut(s) 2564
TspRI CASTG 7 cut(s) 175, 363, 1707, 2886, 3628, 3646, 3973
Tth111I GACNNNGTC 2 cut(s) 37, 705
VneI GTGCAC 1 cut(s) 3070
VpaK11BI GGWCC 5 cut(s) 968, 1169, 1287, 3158, 3167
VspI ATTAAT 1 cut(s) 2667
XapI RAATTY 7 cut(s) 373, 1366, 1526, 1780, 2093, 2709, 3309
XbaI TCTAGA 2 cut(s) 1850, 2009
XceI RCATGY 1 cut(s) 3952
XmaI CCCGGG 1 cut(s) 2564
XmiI GTMKAC 1 cut(s) 2020
XmnI GAANNNNTTC 3 cut(s) 1341, 3432, 3962
ZraI GACGTC 1 cut(s) 166
ZrmI AGTACT 5 cut(s) 1283, 2375, 2408, 3194, 3410
Zsp2I ATGCAT 1 cut(s) 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.