Rh2CG049000
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008072
Physical Location & Seq
Reverse (-)
3884901 .. 3892829
7929 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG049000.1

Sequence Viewer

Length: 4263 bp
ATGTGGAACTCGGCGGAGAACGCGTTTGCGAGGACGGCGTCGTTTCGGGTGGAGGGGGAGGACGAGGAAGCGCTGCGGTGGGCGGCGCTGGAGAGGCTGCCGACATACTCCAGAGTCCGACGTGGCATTTTCAAGAACATTGTGGGAGACACCAAGGAGGTTGACGTCAGCGAGCTGGAGGCTCAGGAGCTGAAGCTACTGCTTGATCGGCTTGTTATCTCGGCTGAAGATGACCCCGAGCACTTCTTTGAGAAAATGCGACTCCGATTTAACGCAGTAGATTTGGAGTTTCCGAAGATCGAGGTGCGGTTTCAGAATTTGAACGTTGAAGCTTTCGTCCATGTGGGAAGCAGAGCACTGCCAACTATTCCGAATTTCGTACTCAATATGACCGAGGCACTTCTGAGGCAATTACGGCTATTAAGGAGCAAGAGAAGCAAATTAACGATTTTAGACAACATTAGTGGGATTATAAGACCTTCAAGATTGACGTTGTTATTGGGTCCACCGAGCTCCGGCAAGACGACGCTACTATTGGCTCTCGCCGGACGCCTTGGAACTGGTCTTCAGATGTCAGGTAGCACAACATACAATGGCCATGGCTTAAACGAGTTTGTGCCGCAGAGGACGTCGGCTTATGTCAGTCAACAGGACTGGTTTGCGGCGGAGATGACTGTAAGGGAGACTCTTGACTTTGCAGGACGGTGTCAAGGTGTTGGATTCAAATATGATATGCTAGTAGAACTAGCAAGAAGAGAAAAGATTGCGGGGATAAAACCTGATGGTGATCTTGATATATTCATGAAGTCATTAGCTCTTGGAGAGCAAGAGACGAGCCTTGTTGTAGAGTACATCATGAAGATTTTAGGGTTGGACATATGCGCTGATACGTTGGTAGGAGACGAAATGCTTAAAGGTATATCAGGGGGCCAAAAGAAGCGGCTTACTACAGGTGAATTGCTAGTAGGTCCAGCAAGAGTGTTGTTCATGGATGAAATATCAACTGGGCTCGATAGTTCCACTACTTATCAAATAATCAAGTATCTTAGGCATTCAACTCGTGCACTTGATTCCACCACTGTTATATCTCTGCTTCAACCTGCTCCAGAGACCTACGAGTTGTTTGATGATGTTATACTTTTGTGTGAGGGCCAGATAGTGTTTCAGGGACCCCGGGAAGCTGCACTTGATTTCTTTTCATACATGGGGTTTAGATGTCCCCGCCGAAAGAATGTGGCAGACTTCTTGCAAGAAGTTATATCAAAGAAGGACCAAGAGCAGTACTGGTCCATTCCTGATCTTCCATACCGCTATATACCACCAGCAAAGTTTGTTGAAGCTTTTCCTTCATTTCAAGATGGGAAGAATTTGTCTGCAGAGTTGAAAGTTCCTTTTGATAAACGCTATAACCACCCTGCTGCTTTGGCGACTTCTCTCTATGGCATGAAGAGGAAGGAGCTTCTCAAGACAAGCTTTAACTGGCAAGTGCTACTGATGAAGCGGAATGCATTTATTTATATTTTCAAATTTGTTCAGCTTCTTTTGGTTGCTTTGATCACAATGAGTGTCTTCTGCCGGACAAAAATGCACCATGATACAATTGATGATGGAGGCTTATATCTCGGGGCACTATACTTTTCCATGGTTATTATTCTTTTTAATGGCTTTACTGAGGTGCCAATGTTGGTGGCCAAGCTTCCAGTGCTTTACAAGCATAGGGACTTACACTTCTACCCAAGTTGGGTCTATACTCTTCCTTCCTGGCTCTTGAGTGTTCCAAATTCACTCATAGAGTCTGGGCTTTGGGTGGCAATCACATACTACGTCATTGGATATGATCCCGCAATTTCTAGATTTTTTGGGCAGTTCTTGGTATATTTCCTTCTGCACCAGATGTCCACAGCTCTCTTTCGTCTTATGGGATCCTTGGGTCGAAATATGATAGTTGCCAACACCTTTGGATCTTTTGCCATGTTGGTTGTAATGGCTCTTGGAGGATACATCATCTCTAGAGATCATATACCAAAATGGTGGATTTGGGGTTTCTGGTTTTCTCCTTTGATGTATGCTCAAAATGCTGCTTCTGTAAATGAATTCCTTGGACACTCCTGGAACACGGAACATGGGCATGAGACCGGTCTTTCATTAGGTCAGTCATTACTAAAAGCACGCAGTTTGTTTCCAGAGAGATACTGGTATTGGATTGGTGCTGGTGCTTTGCTTGGCTATACAGTTTTATTCAATTTACTATTCACATTCTTCCTTGCTTACCTCAACCCCTTGGGGAAGCAACAAGCTGTTGTGTCTAAGGAAGAGCTGGAAGAGAGAGAAAGGAGAAGAAAAGGAGAAAGTGTTGTTATTGAGCTGAGACAGTACTTGAATCATTCACAGTCACTTAATGGAAAATACTTTAAGCAGAGAGGAATGGTCCTCCCATTTCAGCCACTTTCCATGTCTTTCAGCAATATCAATTACTACGTTGATGTCCCTCTGGAATTGAAGCAGCAGGGAATTCAAGAAGAGAGATTGCAGCTCTTGGTTGATGTCACTGGAGCATTTAGGCCGGGGGTGCTTACAGCATTGGTTGGAGTAAGTGGTGCTGGTAAAACCACCCTCATGGACGTATTGGCTGGGCGAAAAACTGGTGGGGTTATAGAAGGAAGCATACATATATCTGGTCATCCAAAAAGACAAGAAACTTTTGCAAGAATTTCGGGTTACTGCGAGCAATCTGATATTCACTCCCCGTGCTTGACTGTTGTTGAATCGCTTCTGTTCTCTGTTTGGCTTCGCTTGCCATCAGATGTTGCCTTGGATACACGGAAGGCTTTTGTAGAGGAGGTGATGGAACTTGTGGAGCTCACTCCATTAAGTGGGGGACTGGTTGGTTTACCGGGGGTGAATGGCTTGTCAACAGAACAACGAAAAAGGCTAACTATAGCAGTGGAGCTGGTCGCTAATCCATCTATAGTATTCATGGATGAACCCACATCTGGGTTAGATGCAAGGTCTGCAGCCATTGTGATGAGGACTGTGAGGAATATAGTCGATACTGGACGTACTATAGTATGCACAATCCACCAGCCTAGCATAGACATTTTTGAATCCTTTGATGAGCTTCTGTTTTTGAAGCGTGGCGGTCAGCTCATATATGCTGGTCCACTTGGACCCAACTCTTGTGAACTCATCAAGTACTTTGAGGCAATTGAAGGAGTGCAAAAGATCAGGCCTGGATATAATCCTGCTGCATGGATGCTTGATGTTACTTCTTCAACTGAAGAAATCCGTCTGGGGGTGGATTTTGCAGAAATTTACAGAAGATCAAATTTGTTTCAACAAAACAAAGACTTGGTTGAAAACCTAAGCAAGCCGAGTACTAATTCCAAAGAATTAAACTTTCCAACTAAATACTGTCAGACATCATTTCAACAATTTCTCACTTGTCTATGGAAGCAAAATCTGTCTTATTGGCGAAACCCTCAGTACACTGCAGTTCGTTTCTTTTACACTGTCATCATCTCCTTGATGCTAGGAACAATTTGTTGGAGATTCGGGGCTAAGAGGGAGACCCAGCAGGACTTATTAAATGCCATGGGATCCTTGTATGCAGCAATCCTATTTAGTGGAGTCACCAATGCCACTGCTGTTCAACCTGTTGTTTCTATAGAAAGATTTGTCTCATATAGAGAGAGAGCTGCTGGGATGTACTCGGCGTTACCATTTGCATTTGCTCAGGTTGCAATAGAGTTCCCTTATGTGTTTGCGCAAGCGGTCATCTACTGCACAATTTTCTACTCCACGGCTGCATTTGACTGGACACTTTTAAAGTTTGTTTGGTACTTATTCTTCATGTATTTCACAATGCTATACTTCACCTTTTACGGAATGATGACAACAGCCGTCACACCAAATCATAACGTAGCTGCCATAATTGCTGCTCCATTTTACATGCTATGGAATCTTTTCAGCGGCTTCATGATTCCTCACAAGAGAATTCCCGTATGGTGGAGATGGTATTACTGGGCAAACCCTGTAGCCTGGAGTCTGTATGGCCTGTTTGTTTCGCAGTACGGTGACGATGACAGTCTTTTGACGCTAGCAGATGGAGAAAGAACAATGCCGGTAAGGCAGTTCCTCAAAGTTGGTTTTGGGTACAGGCATGATTTCCTAGGTGTAGCCGGAATTATGGTCGTCGGTTTCTGCATACTTTTCGCAATAATTTTTGCCTATGCAATAAAATCATTCAACTTCCAGAGGAGATGA

Protein Analysis

1420

Amino Acids

161.38

Weight (kDa)

8.35

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 74 - 126 2.7e-07 ABC-transporter N-terminal
ABC_tran PF00005 151 - 332 1.9e-14 ABC transporter
ABC2_membrane PF01061 488 - 699 3.5e-48 ABC-2 type transporter
PDR_assoc PF08370 704 - 768 9.4e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 848 - 998 1.9e-18 ABC transporter
ABC2_membrane PF01061 1143 - 1357 1.4e-54 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
AatII GACGTC 2 cut(s) 168, 632
Acc16I TGCGCA 1 cut(s) 3765
Acc36I ACCTGC 1 cut(s) 1108
AccB1I GGYRCC 1 cut(s) 1675
AccB7I CCANNNNNTGG 1 cut(s) 2873
AccII CGCG 1 cut(s) 23
AclI AACGTT 1 cut(s) 324
AclWI GGATC 6 cut(s) 1832, 1917, 1930, 1969, 3591, 3604
AcoI YGGCCR 2 cut(s) 595, 1689
AcuI CTGAAG 4 cut(s) 212, 246, 551, 3297
AcyI GRCGYC 4 cut(s) 38, 165, 550, 629
AfeI AGCGCT 1 cut(s) 72
AfiI CCNNNNNNNGG 7 cut(s) 515, 1741, 2873, 2993, 2994, 3292, 4005
AflIII ACRYGT 1 cut(s) 21
AgeI ACCGGT 1 cut(s) 2135
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 4 cut(s) 1760, 2108, 3229, 4037
AjuI GAANNNNNNNTTGG 2 cut(s) 3526, 3558
Alw21I GWGCWC 5 cut(s) 243, 358, 515, 1066, 2862
Alw26I GTCTC 9 cut(s) 141, 677, 824, 894, 1103, 2126, 2362, 3560, 3682
Alw44I GTGCAC 1 cut(s) 1062
AlwI GGATC 6 cut(s) 1832, 1917, 1930, 1969, 3591, 3604
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 3 cut(s) 236, 1173, 1622
Aor51HI AGCGCT 1 cut(s) 72
AoxI GGCC 7 cut(s) 595, 928, 1150, 1689, 2561, 3227, 4051
ApaLI GTGCAC 1 cut(s) 1062
AsiGI ACCGGT 1 cut(s) 2135
Asp700I GAANNNNTTC 3 cut(s) 1341, 2769, 3962
AspA2I CCTAGG 1 cut(s) 4168
AspLEI GCGC 4 cut(s) 73, 88, 884, 3766
AsuC2I CCSGG 4 cut(s) 1174, 1175, 2565, 2895
AsuHPI GGTGA 7 cut(s) 797, 965, 2854, 2911, 3622, 3865, 4085
AsuNHI GCTAGC 1 cut(s) 4096
AvaI CYCGRG 3 cut(s) 236, 1173, 1622
AvaII GGWCC 8 cut(s) 503, 968, 1169, 1270, 1287, 2428, 3158, 3167
AvrII CCTAGG 1 cut(s) 4168
BaeGI GKGCMC 2 cut(s) 1066, 1630
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BalI TGGCCA 2 cut(s) 597, 1691
BamHI GGATCC 2 cut(s) 1922, 3596
BanI GGYRCC 1 cut(s) 1675
BanII GRGCYC 3 cut(s) 515, 1011, 2862
BarI GAAGNNNNNNTAC 6 cut(s) 1866, 1898, 2649, 2681, 3830, 3862
BauI CACGAG 1 cut(s) 1059
BbsI GAAGAC 2 cut(s) 557, 1561
Bbv12I GWGCWC 5 cut(s) 243, 358, 515, 1066, 2862
BccI CCATC 8 cut(s) 776, 1352, 1601, 2806, 2839, 2971, 4005, 4097
BceAI ACGGC 4 cut(s) 51, 431, 3816, 3884
BcgI CGANNNNNNTGC 6 cut(s) 1040, 1074, 2694, 2728, 4192, 4226
BciT130I CCWGG 4 cut(s) 1762, 2110, 3231, 4039
BciVI GTATCC 2 cut(s) 1991, 2809
BclI TGATCA 1 cut(s) 1554
BcnI CCSGG 4 cut(s) 1174, 1175, 2565, 2895
BcoDI GTCTC 9 cut(s) 141, 677, 824, 894, 1103, 2126, 2362, 3560, 3682
BfaI CTAG 9 cut(s) 737, 746, 962, 1851, 2010, 3087, 3530, 4097, 4169
BfmI CTRYAG 9 cut(s) 948, 1374, 2937, 2967, 3012, 3063, 3489, 3663, 4032
BfoI RGCGCY 2 cut(s) 74, 89
BfuAI ACCTGC 1 cut(s) 1108
BfuI GTATCC 2 cut(s) 1991, 2809
BglI GCCNNNNNGGC 1 cut(s) 4126
BlnI CCTAGG 1 cut(s) 4168
BmcAI AGTACT 4 cut(s) 1283, 2375, 3194, 3376
Bme1390I CCNGG 8 cut(s) 1174, 1175, 1762, 2110, 2565, 2895, 3231, 4039
Bme18I GGWCC 8 cut(s) 503, 968, 1169, 1270, 1287, 2428, 3158, 3167
BmeT110I CYCGRG 3 cut(s) 236, 1173, 1622
BmgBI CACGTC 1 cut(s) 122
BmiI GGNNCC 8 cut(s) 504, 929, 1170, 1171, 1677, 1924, 3169, 3598
BmrFI CCNGG 8 cut(s) 1174, 1175, 1762, 2110, 2565, 2895, 3231, 4039
BmrI ACTGGG 2 cut(s) 1014, 4030
BmsI GCATC 3 cut(s) 2992, 3243, 3516
BmtI GCTAGC 1 cut(s) 4100
BmuI ACTGGG 2 cut(s) 1014, 4030
BpiI GAAGAC 2 cut(s) 557, 1561
BpmI CTGGAG 6 cut(s) 94, 110, 197, 1089, 2571, 4060
Bpu10I CCTNAGC 3 cut(s) 183, 3362, 3732
BpuEI CTTGAG 2 cut(s) 1448, 1789
BpuMI CCSGG 4 cut(s) 1174, 1175, 2565, 2895
BsaBI GATNNNNATC 1 cut(s) 786
BsaHI GRCGYC 4 cut(s) 38, 165, 550, 629
BsaI GGTCTC 3 cut(s) 1103, 2126, 3560
BsaWI WCCGGW 1 cut(s) 2135
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 2337, 2367
Bsc4I CCNNNNNNNGG 7 cut(s) 515, 1741, 2873, 2993, 2994, 3292, 4005
Bse118I RCCGGY 2 cut(s) 2135, 4120
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 4 cut(s) 1762, 2110, 3231, 4039
BseGI GGATG 5 cut(s) 997, 2680, 2986, 3258, 3708
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 7 cut(s) 515, 1741, 2873, 2993, 2994, 3292, 4005
BseMII CTCAG 6 cut(s) 197, 395, 1662, 2357, 3494, 3746
BseRI GAGGAG 1 cut(s) 2852
BseSI GKGCMC 2 cut(s) 1066, 1630
BseYI CCCAGC 3 cut(s) 2630, 3570, 3698
BsgI GTGCAG 3 cut(s) 1167, 1871, 3766
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 7 cut(s) 597, 930, 1152, 1691, 2563, 3229, 4053
BshNI GGYRCC 1 cut(s) 1675
BshTI ACCGGT 1 cut(s) 2135
BsiHKAI GWGCWC 5 cut(s) 243, 358, 515, 1066, 2862
BsiHKCI CYCGRG 3 cut(s) 236, 1173, 1622
BsiSI CCGG 9 cut(s) 516, 546, 1174, 1576, 2136, 2564, 2894, 4121, 4179
BslFI GGGAC 5 cut(s) 1182, 1203, 1733, 2471, 2892
BslI CCNNNNNNNGG 7 cut(s) 515, 1741, 2873, 2993, 2994, 3292, 4005
BsmAI GTCTC 9 cut(s) 141, 677, 824, 894, 1103, 2126, 2362, 3560, 3682
BsmBI CGTCTC 2 cut(s) 824, 894
BsmFI GGGAC 5 cut(s) 1182, 1203, 1733, 2471, 2892
BsmI GAATGC 2 cut(s) 1051, 1510
BsnI GGCC 7 cut(s) 597, 930, 1152, 1691, 2563, 3229, 4053
Bso31I GGTCTC 3 cut(s) 1103, 2126, 3560
BsoBI CYCGRG 3 cut(s) 236, 1173, 1622
Bsp1286I GDGCHC 7 cut(s) 243, 358, 515, 1011, 1066, 1630, 2862
Bsp19I CCATGG 3 cut(s) 598, 1641, 3591
BspANI GGCC 7 cut(s) 597, 930, 1152, 1691, 2563, 3229, 4053
BspCNI CTCAG 6 cut(s) 196, 396, 1663, 2358, 3493, 3745
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 3 cut(s) 801, 855, 3975
BspLI GGNNCC 8 cut(s) 504, 929, 1170, 1171, 1677, 1924, 3169, 3598
BspMAI CTGCAG 3 cut(s) 1378, 3016, 3493
BspMI ACCTGC 1 cut(s) 1108
BspOI GCTAGC 1 cut(s) 4100
BspPI GGATC 6 cut(s) 1832, 1917, 1930, 1969, 3591, 3604
BspQI GCTCTTC 1 cut(s) 2307
BspT107I GGYRCC 1 cut(s) 1675
BspTNI GGTCTC 3 cut(s) 1103, 2126, 3560
BsrFI RCCGGY 2 cut(s) 2135, 4120
BssAI RCCGGY 2 cut(s) 2135, 4120
BssNI GRCGYC 4 cut(s) 38, 165, 550, 629
BssSI CACGAG 1 cut(s) 1059
Bst2BI CACGAG 1 cut(s) 1059
Bst2UI CCWGG 4 cut(s) 1762, 2110, 3231, 4039
Bst6I CTCTTC 6 cut(s) 748, 1442, 1758, 2307, 2316, 2514
BstACI GRCGYC 4 cut(s) 38, 165, 550, 629
BstAPI GCANNNNNTGC 1 cut(s) 3011
BstC8I GCNNGC 7 cut(s) 173, 2170, 2726, 2795, 3368, 3768, 4098
BstDSI CCRYGG 4 cut(s) 598, 1641, 3591, 3798
BstF5I GGATG 5 cut(s) 997, 2680, 2986, 3258, 3708
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 2 cut(s) 74, 89
BstHHI GCGC 4 cut(s) 73, 88, 884, 3766
BstMAI GTCTC 9 cut(s) 141, 677, 824, 894, 1103, 2126, 2362, 3560, 3682
BstNI CCWGG 4 cut(s) 1762, 2110, 3231, 4039
BstNSI RCATGY 1 cut(s) 3952
BstSCI CCNGG 8 cut(s) 1172, 1173, 1760, 2108, 2563, 2893, 3229, 4037
BstSFI CTRYAG 9 cut(s) 948, 1374, 2937, 2967, 3012, 3063, 3489, 3663, 4032
BstSLI GKGCMC 2 cut(s) 1066, 1630
BstUI CGCG 1 cut(s) 23
BstV2I GAAGAC 2 cut(s) 557, 1561
BstX2I RGATCY 3 cut(s) 1922, 1961, 3596
BstXI CCANNNNNNTGG 2 cut(s) 2031, 2617
BstYI RGATCY 3 cut(s) 1922, 1961, 3596
BsuI GTATCC 2 cut(s) 1991, 2809
BsuRI GGCC 7 cut(s) 597, 930, 1152, 1691, 2563, 3229, 4053
BtgI CCRYGG 4 cut(s) 598, 1641, 3591, 3798
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 5 cut(s) 997, 2680, 2986, 3258, 3708
BtsI GCAGTG 4 cut(s) 356, 2949, 3486, 3639
BtsIMutI CAGTG 8 cut(s) 356, 1077, 1707, 2547, 2949, 3486, 3507, 3639
BveI ACCTGC 1 cut(s) 1108
Cac8I GCNNGC 7 cut(s) 173, 2170, 2726, 2795, 3368, 3768, 4098
CaiI CAGNNNCTG 1 cut(s) 190
CciI TCATGA 3 cut(s) 801, 855, 3975
CfoI GCGC 4 cut(s) 73, 88, 884, 3766
Cfr10I RCCGGY 2 cut(s) 2135, 4120
Cfr9I CCCGGG 1 cut(s) 1173
CseI GACGC 4 cut(s) 27, 535, 558, 4102
CspAI ACCGGT 1 cut(s) 2135
CspCI CAANNNNNGTGG 4 cut(s) 445, 480, 1668, 1703
DraI TTTAAA 1 cut(s) 3825
EaeI YGGCCR 2 cut(s) 595, 1689
Eam1104I CTCTTC 6 cut(s) 748, 1442, 1758, 2307, 2316, 2514
EarI CTCTTC 6 cut(s) 748, 1442, 1758, 2307, 2316, 2514
EciI GGCGGA 2 cut(s) 29, 680
Ecl136II GAGCTC 2 cut(s) 513, 2860
Eco147I AGGCCT 1 cut(s) 3229
Eco24I GRGCYC 3 cut(s) 515, 1011, 2862
Eco31I GGTCTC 3 cut(s) 1103, 2126, 3560
Eco47I GGWCC 8 cut(s) 503, 968, 1169, 1270, 1287, 2428, 3158, 3167
Eco47III AGCGCT 1 cut(s) 72
Eco53kI GAGCTC 2 cut(s) 513, 2860
Eco57I CTGAAG 4 cut(s) 212, 246, 551, 3297
Eco88I CYCGRG 3 cut(s) 236, 1173, 1622
EcoICRI GAGCTC 2 cut(s) 513, 2860
EcoO109I RGGNCCY 1 cut(s) 1169
EcoRI GAATTC 3 cut(s) 2093, 2511, 3993
EcoRII CCWGG 4 cut(s) 1760, 2108, 3229, 4037
EcoT22I ATGCAT 1 cut(s) 1510
EcoT38I GRGCYC 3 cut(s) 515, 1011, 2862
Esp3I CGTCTC 2 cut(s) 824, 894
FalI AAGNNNNNCTT 4 cut(s) 1170, 1202, 1457, 1489
FaqI GGGAC 5 cut(s) 1182, 1203, 1733, 2471, 2892
FauI CCCGC 3 cut(s) 760, 1229, 1849
FauNDI CATATG 1 cut(s) 878
FbaI TGATCA 1 cut(s) 1554
FokI GGATG 5 cut(s) 1004, 2667, 2993, 3265, 3715
FriOI GRGCYC 3 cut(s) 515, 1011, 2862
FspBI CTAG 9 cut(s) 737, 746, 962, 1851, 2010, 3087, 3530, 4097, 4169
FspI TGCGCA 1 cut(s) 3765
GlaI GCGC 4 cut(s) 72, 87, 883, 3765
GsaI CCCAGC 3 cut(s) 2634, 3574, 3702
GsuI CTGGAG 6 cut(s) 94, 110, 197, 1089, 2571, 4060
HaeII RGCGCY 2 cut(s) 74, 89
HaeIII GGCC 7 cut(s) 597, 930, 1152, 1691, 2563, 3229, 4053
HapII CCGG 9 cut(s) 516, 546, 1174, 1576, 2136, 2564, 2894, 4121, 4179
HgaI GACGC 4 cut(s) 27, 535, 558, 4102
HhaI GCGC 4 cut(s) 73, 88, 884, 3766
Hin1I GRCGYC 4 cut(s) 38, 165, 550, 629
Hin6I GCGC 4 cut(s) 71, 86, 882, 3764
HinP1I GCGC 4 cut(s) 71, 86, 882, 3764
HincII GTYRAC 3 cut(s) 163, 647, 2913
HindII GTYRAC 3 cut(s) 163, 647, 2913
HindIII AAGCTT 4 cut(s) 330, 1338, 1471, 1694
HpaII CCGG 9 cut(s) 516, 546, 1174, 1576, 2136, 2564, 2894, 4121, 4179
HphI GGTGA 7 cut(s) 797, 965, 2854, 2911, 3622, 3865, 4085
Hpy99I CGWCG 5 cut(s) 43, 123, 529, 634, 4196
HpyAV CCTTC 9 cut(s) 489, 1261, 1356, 1447, 1767, 1892, 2651, 2818, 3203
Hsp92I GRCGYC 4 cut(s) 38, 165, 550, 629
HspAI GCGC 4 cut(s) 71, 86, 882, 3764
KflI GGGWCCC 1 cut(s) 1169
Ksp22I TGATCA 1 cut(s) 1554
LguI GCTCTTC 1 cut(s) 2307
LmnI GCTCC 9 cut(s) 187, 426, 518, 1108, 1456, 2552, 2857, 2947, 3943
LweI GCATC 3 cut(s) 2992, 3243, 3516
MaeI CTAG 9 cut(s) 737, 746, 962, 1851, 2010, 3087, 3530, 4097, 4169
MaeIII GTNAC 8 cut(s) 2391, 2545, 2717, 3262, 3628, 3714, 3901, 4073
MfeI CAATTG 2 cut(s) 1599, 3204
MflI RGATCY 3 cut(s) 1922, 1961, 3596
MhlI GDGCHC 7 cut(s) 243, 358, 515, 1011, 1066, 1630, 2862
MlsI TGGCCA 2 cut(s) 597, 1691
MluI ACGCGT 1 cut(s) 21
MluNI TGGCCA 2 cut(s) 597, 1691
MlyI GAGTC 6 cut(s) 123, 255, 679, 1802, 3636, 4051
MmeI TCCRAC 6 cut(s) 142, 697, 852, 2566, 3425, 3524
Mox20I TGGCCA 2 cut(s) 597, 1691
Mph1103I ATGCAT 1 cut(s) 1510
MroXI GAANNNNTTC 3 cut(s) 1341, 2769, 3962
MscI TGGCCA 2 cut(s) 597, 1691
MslI CAYNNNNRTG 3 cut(s) 2127, 2615, 3023
Msp20I TGGCCA 2 cut(s) 597, 1691
MspA1I CMGCKG 1 cut(s) 3969
MspI CCGG 9 cut(s) 516, 546, 1174, 1576, 2136, 2564, 2894, 4121, 4179
MspR9I CCNGG 8 cut(s) 1174, 1175, 1762, 2110, 2565, 2895, 3231, 4039
MunI CAATTG 2 cut(s) 1599, 3204
Mva1269I GAATGC 2 cut(s) 1051, 1510
MvaI CCWGG 4 cut(s) 1762, 2110, 3231, 4039
MvnI CGCG 1 cut(s) 23
NciI CCSGG 4 cut(s) 1174, 1175, 2565, 2895
NcoI CCATGG 3 cut(s) 598, 1641, 3591
NdeI CATATG 1 cut(s) 878
NheI GCTAGC 1 cut(s) 4096
NlaIV GGNNCC 8 cut(s) 504, 929, 1170, 1171, 1677, 1924, 3169, 3598
NmeAIII GCCGAG 3 cut(s) 200, 3396, 3689
NmuCI GTSAC 5 cut(s) 2391, 2545, 3628, 3901, 4073
NsbI TGCGCA 1 cut(s) 3765
NsiI ATGCAT 1 cut(s) 1510
NspI RCATGY 1 cut(s) 3952
PagI TCATGA 3 cut(s) 801, 855, 3975
PceI AGGCCT 1 cut(s) 3229
PciSI GCTCTTC 1 cut(s) 2307
PctI GAATGC 2 cut(s) 1051, 1510
PdmI GAANNNNTTC 3 cut(s) 1341, 2769, 3962
PfeI GAWTC 8 cut(s) 720, 1070, 2380, 2765, 3104, 3549, 3958, 3979
PflFI GACNNNGTC 2 cut(s) 37, 705
PflMI CCANNNNNTGG 1 cut(s) 2873
PfoI TCCNGGA 1 cut(s) 2108
PinAI ACCGGT 1 cut(s) 2135
PleI GAGTC 6 cut(s) 122, 255, 679, 1801, 3635, 4050
PpsI GAGTC 6 cut(s) 122, 255, 679, 1801, 3635, 4050
PpuMI RGGWCCY 1 cut(s) 1169
PsiI TTATAA 1 cut(s) 473
Psp124BI GAGCTC 2 cut(s) 515, 2862
Psp1406I AACGTT 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 1169
Psp6I CCWGG 4 cut(s) 1760, 2108, 3229, 4037
PspFI CCCAGC 3 cut(s) 2630, 3570, 3698
PspGI CCWGG 4 cut(s) 1760, 2108, 3229, 4037
PspN4I GGNNCC 8 cut(s) 504, 929, 1170, 1171, 1677, 1924, 3169, 3598
PspPPI RGGWCCY 1 cut(s) 1169
PstI CTGCAG 3 cut(s) 1378, 3016, 3493
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 3 cut(s) 1922, 1961, 3596
PsyI GACNNNGTC 2 cut(s) 37, 705
RseI CAYNNNNRTG 3 cut(s) 2127, 2615, 3023
SacI GAGCTC 2 cut(s) 515, 2862
SapI GCTCTTC 1 cut(s) 2307
ScaI AGTACT 4 cut(s) 1283, 2375, 3194, 3376
SchI GAGTC 6 cut(s) 123, 255, 679, 1802, 3636, 4051
ScrFI CCNGG 8 cut(s) 1174, 1175, 1762, 2110, 2565, 2895, 3231, 4039
SduI GDGCHC 7 cut(s) 243, 358, 515, 1011, 1066, 1630, 2862
SfaNI GCATC 3 cut(s) 2992, 3243, 3516
SfcI CTRYAG 9 cut(s) 948, 1374, 2937, 2967, 3012, 3063, 3489, 3663, 4032
SinI GGWCC 8 cut(s) 503, 968, 1169, 1270, 1287, 2428, 3158, 3167
SmaI CCCGGG 1 cut(s) 1175
SmiMI CAYNNNNRTG 3 cut(s) 2127, 2615, 3023
SmlI CTYRAG 2 cut(s) 1463, 1768
SmoI CTYRAG 2 cut(s) 1463, 1768
SseBI AGGCCT 1 cut(s) 3229
SspMI CTAG 9 cut(s) 737, 746, 962, 1851, 2010, 3087, 3530, 4097, 4169
SstI GAGCTC 2 cut(s) 515, 2862
StuI AGGCCT 1 cut(s) 3229
StyD4I CCNGG 8 cut(s) 1172, 1173, 1760, 2108, 2563, 2893, 3229, 4037
TaqI TCGA 4 cut(s) 300, 1011, 1933, 3048
TaqII GACCGA 1 cut(s) 407
TatI WGTACW 7 cut(s) 849, 1281, 2373, 3192, 3374, 3483, 3705
TauI GCSGC 5 cut(s) 86, 622, 665, 943, 3972
TfiI GAWTC 8 cut(s) 720, 1070, 2380, 2765, 3104, 3549, 3958, 3979
TscAI CASTG 8 cut(s) 363, 1084, 1707, 2554, 2949, 3493, 3514, 3646
TseFI GTSAC 5 cut(s) 2391, 2545, 3628, 3901, 4073
Tsp45I GTSAC 5 cut(s) 2391, 2545, 3628, 3901, 4073
TspGWI ACGGA 4 cut(s) 2132, 2836, 3275, 3897
TspMI CCCGGG 1 cut(s) 1173
TspRI CASTG 8 cut(s) 363, 1084, 1707, 2554, 2949, 3493, 3514, 3646
Tth111I GACNNNGTC 2 cut(s) 37, 705
Van91I CCANNNNNTGG 1 cut(s) 2873
VneI GTGCAC 1 cut(s) 1062
VpaK11BI GGWCC 8 cut(s) 503, 968, 1169, 1270, 1287, 2428, 3158, 3167
XbaI TCTAGA 2 cut(s) 1850, 2009
XceI RCATGY 1 cut(s) 3952
XcmI CCANNNNNNNNNTGG 1 cut(s) 2190
XmaI CCCGGG 1 cut(s) 1173
XmaJI CCTAGG 1 cut(s) 4168
XmnI GAANNNNTTC 3 cut(s) 1341, 2769, 3962
XspI CTAG 9 cut(s) 737, 746, 962, 1851, 2010, 3087, 3530, 4097, 4169
ZraI GACGTC 2 cut(s) 166, 630
ZrmI AGTACT 4 cut(s) 1283, 2375, 3194, 3376
Zsp2I ATGCAT 1 cut(s) 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.