Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0008072

Physical Location & Seq

Genomic Coordinates Forward (+)

301878 .. 304177

Gene Structure 2300 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0002900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGGCCAAGGCAGTCACTTACAGCGTGACCACCTTAGGAGCCAAACCAGATGGCAAGACCGACTCAACTAAGGCCTTCCTTTCTGCATGGGCTAAAGCTTGTGCCTCGGTGAAACCAGCTGTGATTTACGTGCCGGCAGGGAGGTTCTTGCTCCGCAATGCCCTGTTCAGCGGACCATGCAAGAACAGCCTCATCACCTTCCGCATTGCTGGCACCCTCGTTGCTCCGTCCGATTACCGGGTCATTGGAAATGCCGGTAACTGGCTCCTCTTTCGGCAAGTCAACGGTGTTACCATTTCCGGTGGAATTCTTGACGGTCAAGGCACTGGTTTGTGGGCGTGCAAGAACTCCGGCAAGAGTTGCCCCACTGGAGCCACGACATTGAGTTTTTCCAACTCCAACAATATTGTGGTTAGCGGATTGGTATCTCTAAACAGCCAAATGTTTCACATAGTCATCAACGGTTGCAACAATGTTAAAATGCAAGGGGTTAAAGTATCAGCCTCCGGCAACAGCCCTAACACCGACGGTATCCATGTTCAAATGTCGTCGGGTGTTACCATTCTCAACTCAAAGATCTCTACCGGTGATGATTGTGTATCAATTGGTCCCGGCACTTCTAACTTGTGGATTGAAAATGTTGCATGCGGACCGGGTCACGGAATTAGCATTGGGAGTCTAGGCAAAGACCTACAGGAAGCTGGTGTGCAAAATGTAACGGTCAAAACAGTGACATTTACCAATACTCAAAATGGTGTGAGGATCAAGTCCTGGGGGAGGGCTAGCACTGGATTTGCCAGGAACATTCTTTTCCAACATGCTGTCATGATTAACGTTCAAAATCCGATAGTCATCGATCAACAATATTGTCCCGACAAGAAAGGCTGCCCTGGCCAGGTTTCTGGAGTTAAAATCAGTGATGTGACATATCAAGACATTCATGGTACATCGGCAACAGAAGTTGCAGTGAAATTCGATTGTAGTTCAAAGTACCCGTGTAGCAGAATCAGATTAGAGAATGTGAAGCTCATTTACAAGAATAACCAAGGAGCTCTAGCTTCATGTAGCAATGCTGGTGGAACATCTGCGGGTACGGTGCAGCCTACAAGTTGTCTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006855 GO:0007275 GO:0008150 GO:0015399 GO:0015405 GO:0015893 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031224 GO:0032501 GO:0032502 GO:0042221 GO:0042335 GO:0042493 GO:0042623 GO:0042626 GO:0043492 GO:0044425 GO:0044464 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

372

Amino Acids

38.85

Weight (kDa)

9.33

Isoelectric Point (pI)

26.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pect-lyase_RHGA_epim	PF12708	8 - 259	2.4e-14	Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28	PF00295	34 - 358	1.7e-87	Glycosyl hydrolases family 28

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008072)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26910
fragaria_vesca	FvH4_1g04210 FvH4_1g04210 FvH4_1g04210 FvH4_1g04210 FvH4_1g04210 FvH4_1g04210 FvH4_1g04210
malus_domestica	MD02G1040300.v1.1 MD15G1145300.v1.1
prunus_persica	Prupe.7G235300_v2.0.a1
pyrus_communis	pycom02g03360 pycom15g16260
rosa_chinensis	RchiOBHm_Chr2g0089741
rosa_laevigata	RLG00000016052
rosa_multiflora	Rmu_sc0000665.1_g000015
rosa_roxburghii	Rroxscaffold_2G00151650
rosa_rugosa	Rorug02G0002800 Rorug02G0002900
rosa_samantha	Rh2AG048100 Rh2BG046900 Rh2CG049000 Rh2DG048300
rosa_wichuraiana	Rw2G004230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	212
AciI	CCGC	6 cut(s)	154, 171, 202, 417, 648, 1088
AclI	AACGTT	1 cut(s)	834
AclWI	GGATC	1 cut(s)	770
AcoI	YGGCCR	2 cut(s)	3, 892
AcsI	RAATTY	2 cut(s)	306, 971
AfaI	GTAC	3 cut(s)	946, 992, 1093
AfiI	CCNNNNNNNGG	5 cut(s)	237, 261, 659, 777, 895
AgeI	ACCGGT	1 cut(s)	584
AgsI	TTSAA	4 cut(s)	542, 635, 839, 987
AjnI	CCWGG	4 cut(s)	770, 797, 889, 894
AluBI	AGCT	6 cut(s)	98, 119, 701, 1027, 1052, 1058
AluI	AGCT	6 cut(s)	98, 119, 701, 1027, 1052, 1058
Alw21I	GWGCWC	1 cut(s)	1054
AlwI	GGATC	1 cut(s)	770
AoxI	GGCC	3 cut(s)	3, 72, 892
ApeKI	GCWGC	2 cut(s)	885, 1099
ApoI	RAATTY	2 cut(s)	306, 971
AsiGI	ACCGGT	1 cut(s)	584
AspS9I	GGNCC	3 cut(s)	173, 608, 650
AsuC2I	CCSGG	3 cut(s)	239, 612, 654
AsuHPI	GGTGA	3 cut(s)	121, 187, 599
AsuNHI	GCTAGC	1 cut(s)	782
AvaII	GGWCC	3 cut(s)	173, 608, 650
AxyI	CCTNAGG	1 cut(s)	34
BalI	TGGCCA	2 cut(s)	5, 894
BanI	GGYRCC	1 cut(s)	212
BanII	GRGCYC	1 cut(s)	1054
BarI	GAAGNNNNNNTAC	2 cut(s)	1016, 1048
Bbv12I	GWGCWC	1 cut(s)	1054
BbvI	GCAGC	2 cut(s)	872, 1111
BccI	CCATC	1 cut(s)	44
BciT130I	CCWGG	4 cut(s)	772, 799, 891, 896
BciVI	GTATCC	1 cut(s)	542
BcnI	CCSGG	3 cut(s)	239, 612, 654
BfaI	CTAG	3 cut(s)	680, 783, 1055
BfmI	CTRYAG	2 cut(s)	692, 1115
BfuI	GTATCC	1 cut(s)	542
BglII	AGATCT	1 cut(s)	576
BisI	GCNGC	2 cut(s)	886, 1100
BlsI	GCNGC	2 cut(s)	887, 1101
Bme1390I	CCNGG	7 cut(s)	239, 612, 654, 772, 799, 891, 896
Bme18I	GGWCC	3 cut(s)	173, 608, 650
BmgT120I	GGNCC	3 cut(s)	173, 608, 650
BmiI	GGNNCC	5 cut(s)	40, 214, 266, 373, 610
BmrFI	CCNGG	7 cut(s)	239, 612, 654, 772, 799, 891, 896
BmtI	GCTAGC	1 cut(s)	786
BpmI	CTGGAG	2 cut(s)	390, 924
BpuMI	CCSGG	3 cut(s)	239, 612, 654
Bsa29I	ATCGAT	1 cut(s)	855
BsaAI	YACGTR	1 cut(s)	130
BsaBI	GATNNNNATC	2 cut(s)	424, 851
BsaJI	CCNNGG	5 cut(s)	6, 105, 771, 889, 1045
BsaWI	WCCGGW	2 cut(s)	299, 584
Bsc4I	CCNNNNNNNGG	5 cut(s)	237, 261, 659, 777, 895
Bse118I	RCCGGY	3 cut(s)	133, 254, 584
Bse1I	ACTGG	4 cut(s)	266, 331, 373, 793
Bse21I	CCTNAGG	1 cut(s)	34
Bse3DI	GCAATG	3 cut(s)	163, 204, 1075
Bse8I	GATNNNNATC	2 cut(s)	424, 851
BseBI	CCWGG	4 cut(s)	772, 799, 891, 896
BseCI	ATCGAT	1 cut(s)	855
BseDI	CCNNGG	5 cut(s)	6, 105, 771, 889, 1045
BseJI	GATNNNNATC	2 cut(s)	424, 851
BseLI	CCNNNNNNNGG	5 cut(s)	237, 261, 659, 777, 895
BseMI	GCAATG	3 cut(s)	163, 204, 1075
BseNI	ACTGG	4 cut(s)	266, 331, 373, 793
BseRI	GAGGAG	1 cut(s)	257
BseXI	GCAGC	2 cut(s)	872, 1111
BsgI	GTGCAG	1 cut(s)	1118
BshFI	GGCC	3 cut(s)	5, 74, 894
BshNI	GGYRCC	1 cut(s)	212
BshTI	ACCGGT	1 cut(s)	584
BshVI	ATCGAT	1 cut(s)	855
BsiHKAI	GWGCWC	1 cut(s)	1054
BsiSI	CCGG	9 cut(s)	134, 238, 255, 300, 351, 507, 585, 612, 653
BslFI	GGGAC	2 cut(s)	594, 855
BslI	CCNNNNNNNGG	5 cut(s)	237, 261, 659, 777, 895
BsmFI	GGGAC	2 cut(s)	594, 855
BsnI	GGCC	3 cut(s)	5, 74, 894
Bsp1286I	GDGCHC	1 cut(s)	1054
Bsp143I	GATC	3 cut(s)	576, 762, 856
BspACI	CCGC	6 cut(s)	154, 171, 202, 417, 648, 1088
BspANI	GGCC	3 cut(s)	5, 74, 894
BspDI	ATCGAT	1 cut(s)	855
BspHI	TCATGA	1 cut(s)	825
BspLI	GGNNCC	5 cut(s)	40, 214, 266, 373, 610
BspOI	GCTAGC	1 cut(s)	786
BspPI	GGATC	1 cut(s)	770
BspT107I	GGYRCC	1 cut(s)	212
BsrDI	GCAATG	3 cut(s)	163, 204, 1075
BsrFI	RCCGGY	3 cut(s)	133, 254, 584
BsrI	ACTGG	4 cut(s)	266, 331, 373, 793
BssAI	RCCGGY	3 cut(s)	133, 254, 584
BssECI	CCNNGG	5 cut(s)	6, 105, 771, 889, 1045
BssMI	GATC	3 cut(s)	576, 762, 856
BssT1I	CCWWGG	2 cut(s)	6, 1045
Bst2UI	CCWGG	4 cut(s)	772, 799, 891, 896
Bst4CI	ACNGT	7 cut(s)	287, 317, 464, 530, 721, 730, 1096
BstAPI	GCANNNNNTGC	1 cut(s)	360
BstBAI	YACGTR	1 cut(s)	130
BstC8I	GCNNGC	5 cut(s)	135, 211, 340, 646, 784
BstDEI	CTNAG	2 cut(s)	34, 69
BstENI	CCTNNNNNAGG	1 cut(s)	775
BstKTI	GATC	3 cut(s)	579, 765, 859
BstMBI	GATC	3 cut(s)	576, 762, 856
BstMWI	GCNNNNNNNGC	5 cut(s)	177, 186, 210, 360, 891
BstNI	CCWGG	4 cut(s)	772, 799, 891, 896
BstNSI	RCATGY	2 cut(s)	648, 821
BstSCI	CCNGG	7 cut(s)	237, 610, 652, 770, 797, 889, 894
BstSFI	CTRYAG	2 cut(s)	692, 1115
BstV1I	GCAGC	2 cut(s)	872, 1111
BstX2I	RGATCY	1 cut(s)	576
BstXI	CCANNNNNNTGG	1 cut(s)	902
BstYI	RGATCY	1 cut(s)	576
Bsu15I	ATCGAT	1 cut(s)	855
Bsu36I	CCTNAGG	1 cut(s)	34
BsuI	GTATCC	1 cut(s)	542
BsuRI	GGCC	3 cut(s)	5, 74, 894
BsuTUI	ATCGAT	1 cut(s)	855
BtsI	GCAGTG	1 cut(s)	972
BtsIMutI	CAGTG	6 cut(s)	324, 366, 735, 786, 922, 972
Cac8I	GCNNGC	5 cut(s)	135, 211, 340, 646, 784
CciI	TCATGA	1 cut(s)	825
Cfr10I	RCCGGY	3 cut(s)	133, 254, 584
Cfr13I	GGNCC	3 cut(s)	173, 608, 650
ClaI	ATCGAT	1 cut(s)	855
CpoI	CGGWCCG	1 cut(s)	650
Csp6I	GTAC	3 cut(s)	945, 991, 1092
CspAI	ACCGGT	1 cut(s)	584
CspCI	CAANNNNNGTGG	2 cut(s)	1057, 1092
CspI	CGGWCCG	1 cut(s)	650
CviAII	CATG	8 cut(s)	87, 177, 536, 645, 818, 826, 941, 1062
CviQI	GTAC	3 cut(s)	945, 991, 1092
DdeI	CTNAG	2 cut(s)	34, 69
DpnI	GATC	3 cut(s)	578, 764, 858
DpnII	GATC	3 cut(s)	576, 762, 856
EaeI	YGGCCR	2 cut(s)	3, 892
Ecl136II	GAGCTC	1 cut(s)	1052
Eco130I	CCWWGG	2 cut(s)	6, 1045
Eco147I	AGGCCT	1 cut(s)	74
Eco24I	GRGCYC	1 cut(s)	1054
Eco47I	GGWCC	3 cut(s)	173, 608, 650
Eco53kI	GAGCTC	1 cut(s)	1052
Eco81I	CCTNAGG	1 cut(s)	34
EcoICRI	GAGCTC	1 cut(s)	1052
EcoNI	CCTNNNNNAGG	1 cut(s)	775
EcoRI	GAATTC	1 cut(s)	306
EcoRII	CCWGG	4 cut(s)	770, 797, 889, 894
EcoT14I	CCWWGG	2 cut(s)	6, 1045
EcoT38I	GRGCYC	1 cut(s)	1054
ErhI	CCWWGG	2 cut(s)	6, 1045
FaeI	CATG	8 cut(s)	90, 180, 539, 648, 821, 829, 944, 1065
FaqI	GGGAC	2 cut(s)	594, 855
FatI	CATG	8 cut(s)	86, 176, 535, 644, 817, 825, 940, 1061
FauI	CCCGC	1 cut(s)	1081
Fnu4HI	GCNGC	2 cut(s)	886, 1100
FriOI	GRGCYC	1 cut(s)	1054
Fsp4HI	GCNGC	2 cut(s)	886, 1100
FspBI	CTAG	3 cut(s)	680, 783, 1055
GluI	GCNGC	2 cut(s)	886, 1100
GsuI	CTGGAG	2 cut(s)	390, 924
HaeIII	GGCC	3 cut(s)	5, 74, 894
HapII	CCGG	9 cut(s)	134, 238, 255, 300, 351, 507, 585, 612, 653
Hin1II	CATG	8 cut(s)	90, 180, 539, 648, 821, 829, 944, 1065
HincII	GTYRAC	1 cut(s)	283
HindII	GTYRAC	1 cut(s)	283
HindIII	AAGCTT	1 cut(s)	96
HinfI	GANTC	3 cut(s)	62, 676, 1005
HpaII	CCGG	9 cut(s)	134, 238, 255, 300, 351, 507, 585, 612, 653
HphI	GGTGA	3 cut(s)	121, 187, 599
Hpy166II	GTNNAC	1 cut(s)	283
Hpy188I	TCNGA	3 cut(s)	232, 846, 1010
Hpy188III	TCNNGA	5 cut(s)	311, 826, 872, 903, 932
Hpy8I	GTNNAC	1 cut(s)	283
Hpy99I	CGWCG	2 cut(s)	530, 553
HpyAV	CCTTC	2 cut(s)	85, 208
HpyCH4III	ACNGT	7 cut(s)	287, 317, 464, 530, 721, 730, 1096
HpyCH4IV	ACGT	2 cut(s)	129, 834
HpyCH4V	TGCA	9 cut(s)	86, 180, 342, 468, 484, 644, 709, 965, 1099
HpyF10VI	GCNNNNNNNGC	5 cut(s)	177, 186, 210, 360, 891
HpyF3I	CTNAG	2 cut(s)	34, 69
HpySE526I	ACGT	2 cut(s)	129, 834
Hsp92II	CATG	8 cut(s)	90, 180, 539, 648, 821, 829, 944, 1065
KroI	GCCGGC	1 cut(s)	133
KroNI	GCCGGC	1 cut(s)	135
Kzo9I	GATC	3 cut(s)	576, 762, 856
LmnI	GCTCC	6 cut(s)	38, 156, 229, 270, 371, 1049
Lsp1109I	GCAGC	2 cut(s)	872, 1111
MaeI	CTAG	3 cut(s)	680, 783, 1055
MaeII	ACGT	2 cut(s)	129, 834
MaeIII	GTNAC	9 cut(s)	13, 25, 257, 289, 556, 656, 715, 730, 922
MalI	GATC	3 cut(s)	578, 764, 858
MboI	GATC	3 cut(s)	576, 762, 856
MfeI	CAATTG	1 cut(s)	603
MflI	RGATCY	1 cut(s)	576
MhlI	GDGCHC	1 cut(s)	1054
MlsI	TGGCCA	2 cut(s)	5, 894
MluCI	AATT	4 cut(s)	306, 603, 663, 971
MluNI	TGGCCA	2 cut(s)	5, 894
MlyI	GAGTC	2 cut(s)	56, 685
MmeI	TCCRAC	3 cut(s)	417, 423, 838
MnlI	CCTC	8 cut(s)	115, 135, 200, 227, 278, 514, 753, 771
Mox20I	TGGCCA	2 cut(s)	5, 894
MroNI	GCCGGC	1 cut(s)	133
MscI	TGGCCA	2 cut(s)	5, 894
MseI	TTAA	4 cut(s)	477, 492, 831, 909
Msp20I	TGGCCA	2 cut(s)	5, 894
MspA1I	CMGCKG	2 cut(s)	119, 171
MspI	CCGG	9 cut(s)	134, 238, 255, 300, 351, 507, 585, 612, 653
MspR9I	CCNGG	7 cut(s)	239, 612, 654, 772, 799, 891, 896
MunI	CAATTG	1 cut(s)	603
MvaI	CCWGG	4 cut(s)	772, 799, 891, 896
MwoI	GCNNNNNNNGC	5 cut(s)	177, 186, 210, 360, 891
NaeI	GCCGGC	1 cut(s)	135
NciI	CCSGG	3 cut(s)	239, 612, 654
NdeII	GATC	3 cut(s)	576, 762, 856
NgoMIV	GCCGGC	1 cut(s)	133
NheI	GCTAGC	1 cut(s)	782
NlaIII	CATG	8 cut(s)	90, 180, 539, 648, 821, 829, 944, 1065
NlaIV	GGNNCC	5 cut(s)	40, 214, 266, 373, 610
NmuCI	GTSAC	5 cut(s)	13, 25, 656, 730, 922
NspI	RCATGY	2 cut(s)	648, 821
PaeI	GCATGC	1 cut(s)	648
PagI	TCATGA	1 cut(s)	825
PceI	AGGCCT	1 cut(s)	74
PdiI	GCCGGC	1 cut(s)	135
PfeI	GAWTC	1 cut(s)	1005
PflFI	GACNNNGTC	1 cut(s)	654
PinAI	ACCGGT	1 cut(s)	584
PkrI	GCNGC	2 cut(s)	887, 1101
PleI	GAGTC	2 cut(s)	56, 684
PpsI	GAGTC	2 cut(s)	56, 684
Ppu21I	YACGTR	1 cut(s)	130
Psp124BI	GAGCTC	1 cut(s)	1054
Psp1406I	AACGTT	1 cut(s)	834
Psp6I	CCWGG	4 cut(s)	770, 797, 889, 894
PspGI	CCWGG	4 cut(s)	770, 797, 889, 894
PspN4I	GGNNCC	5 cut(s)	40, 214, 266, 373, 610
PspPI	GGNCC	3 cut(s)	173, 608, 650
PsuI	RGATCY	1 cut(s)	576
PsyI	GACNNNGTC	1 cut(s)	654
PvuII	CAGCTG	1 cut(s)	119
RsaI	GTAC	3 cut(s)	946, 992, 1093
RsaNI	GTAC	3 cut(s)	945, 991, 1092
Rsr2I	CGGWCCG	1 cut(s)	650
RsrII	CGGWCCG	1 cut(s)	650
SacI	GAGCTC	1 cut(s)	1054
SaqAI	TTAA	4 cut(s)	477, 492, 831, 909
SatI	GCNGC	2 cut(s)	886, 1100
Sau3AI	GATC	3 cut(s)	576, 762, 856
Sau96I	GGNCC	3 cut(s)	173, 608, 650
SchI	GAGTC	2 cut(s)	56, 685
ScrFI	CCNGG	7 cut(s)	239, 612, 654, 772, 799, 891, 896
SduI	GDGCHC	1 cut(s)	1054
SfcI	CTRYAG	2 cut(s)	692, 1115
SinI	GGWCC	3 cut(s)	173, 608, 650
SphI	GCATGC	1 cut(s)	648
Sse9I	AATT	4 cut(s)	306, 603, 663, 971
SseBI	AGGCCT	1 cut(s)	74
SsiI	CCGC	6 cut(s)	154, 171, 202, 417, 648, 1088
SspI	AATATT	2 cut(s)	406, 866
SspMI	CTAG	3 cut(s)	680, 783, 1055
SstI	GAGCTC	1 cut(s)	1054
StuI	AGGCCT	1 cut(s)	74
StyD4I	CCNGG	7 cut(s)	237, 610, 652, 770, 797, 889, 894
StyI	CCWWGG	2 cut(s)	6, 1045
TaaI	ACNGT	7 cut(s)	287, 317, 464, 530, 721, 730, 1096
TaiI	ACGT	2 cut(s)	132, 837
TaqI	TCGA	2 cut(s)	855, 975
TaqII	GACCGA	1 cut(s)	74
TasI	AATT	4 cut(s)	306, 603, 663, 971
TfiI	GAWTC	1 cut(s)	1005
Tru1I	TTAA	4 cut(s)	477, 492, 831, 909
Tru9I	TTAA	4 cut(s)	477, 492, 831, 909
TscAI	CASTG	6 cut(s)	331, 373, 735, 793, 922, 972
TseFI	GTSAC	5 cut(s)	13, 25, 656, 730, 922
TseI	GCWGC	2 cut(s)	885, 1099
Tsp45I	GTSAC	5 cut(s)	13, 25, 656, 730, 922
TspDTI	ATGAA	2 cut(s)	929, 1050
TspGWI	ACGGA	2 cut(s)	216, 675
TspRI	CASTG	6 cut(s)	331, 373, 735, 793, 922, 972
Tth111I	GACNNNGTC	1 cut(s)	654
VpaK11BI	GGWCC	3 cut(s)	173, 608, 650
XagI	CCTNNNNNAGG	1 cut(s)	775
XapI	RAATTY	2 cut(s)	306, 971
XceI	RCATGY	2 cut(s)	648, 821
XcmI	CCANNNNNNNNNTGG	1 cut(s)	406
XspI	CTAG	3 cut(s)	680, 783, 1055

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.