RLG00000016052
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008072
Physical Location & Seq
Reverse (-)
3501034 .. 3507982
6949 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016052

Sequence Viewer

Length: 4266 bp
ATGTGGAACTCGGCGGAGAACGCGTTCGCGAGGACGGCGTCGTTTCGGGTGGAGGGCGAGGACGAGGAGGCGCTGCGGTGGGCGGCGCTGGAGAGGCTGCCGACATTCTCCAGAGTCCGACGTGGCATTTTCAAGAACATTGTGGGAGATACCAAGGAGGTTGACGTCAGCGAGCTGGAGGCTCAGGAGCTGAAGCTACTGCTTGATCGGCTTGTCAACTCATCGGCTGAAGATGACCCCGAGCGCTTCTTTGAGAAAATGCGACTCAGATTTAACGCAGTAGATTTGGAGTTTCCGAAGATCGAGGTGCGGTTTCAGAATTTGAACGTTGAAGCTTTCGTCCATGTGGGAAGCAGAGCACTGCCGACTATTCCGAATTTCGTATTCAATATGACCGAGGCACTTCTGAGGCAATTACGGCTATTAAGGAGCAAGAGAAGCAAATTAACGATTTTAGACAACATTAGTGGGATTATAAGACCTTCAAGATTGACTTTGTTATTGGGTCCACCGAGCTCCGGCAAGACAACGCTACTATTGGCTCTCGCCGGACGCCTTGGAACTGGTCTTCAGATGTCAGGTAGCACAACATACAATGGCCATGGCTTAAACGAGTTTGTGCCGCAGAGGACGTCGGCTTATGTCAGTCAACAGGACTGGTTTGCGGCGGAGATGACTGTAAGGGAGACTCTTGACTTTGCAGGACGGTGTCAAGGTGTTGGATTCAAATATGATATGCTAGTAGAACTAGCAAGAAGGGAAAAGATTGCAGGAATAAAACCCGATGGTGATCTTGATATATTCATGAAGTCATTAGCTCTTGGAGAGCAAGAGACAAGCCTTGTGGTAGAGTACATCATGAAGATTTTAGGGTTGGACATATGCGCTGATACGTTGGTAGGAGATGAAATGCTTAAAGGTATATCAGGGGGCCAAAAGAAGCGGCTTACTACAGGTGAATTATTAGTAGGTCCAGCAAGAGTGTTGTTCATGGATGAAATATCAACCGGGCTCGATAGTTCCACTACTTATCAAATCATCAAGTATCTTAGGCATTCAACTCGTGCACTTGATTCCACCACTGTTATATCTCTGCTTCAACCTGCTCCAGAGACCTACGAGTTGTTTGATGATGTTATACTTTTGTGTGAGGGCCAGATCGTGTTTCAGGGACCCCGGGAAGCGGCTCTTGATTTCTTTTCATACATGGGGTTTAGATGTCCCCGCCGAAAGAATGTGGCAGACTTCTTGCAAGAAGTTATATCAGAGAAGGACCAAGAGCAGTACTGGTCCAATCCTGATCTTCCATACCGCTATATACCACCGGCAAAATTTGTTGAAGCTTTTCCTTCATTTCAAGATGGGAAGAATTTGTCTGAAGAGTTGAAAGTTCCTTTTGATAAACGCTATAACCACCCTGCAGCTTTGGCGACTTCTCTCTATGGCATGAAGAGGAAGGAGCTTCTCAAGACAAGCTTTAACTGGCAAGTGCTACTGATGAAGCGGAATGCATTTATTTATATTTTCAAATTTGTTCAGCTTATTTTGGTTGCTTTGATCACAATGAGTGTCTTCTGCCGGACAAAAATGCACCATGATACAATTGATGATGGAGGCTTATATCTTGGGGCACTGTACTTTTCCATGGTTATTATTCTTTTTAATGGCTTTACGGAGGTGCCAATGTTGGTGGCCAAGCTTCCAGTGCTTTACAAGCATAGGGACTTACACTTCTACCCAAGTTGGGTCTATACGCTTCCTTCCTGGCTCTTGAGTATTCCAAATTCACTCATAGAGTCTGGACTTTGGGTGGCAATCACATACTACGTCATTGGATACGATCCCGCAATTTCTAGATTTTTTGGGCAGTTCTTGGTATATTTCCTTCTGCACCAGATGTCCACAGCTCTCTTTCGTCTTATGGGATCCTTGGGTCGAAATATGATAGTTGCCAACACCTTTGGATCTTTTGCCATGTTGGTTGTAATGGCTCTTGGAGGATACATCATCTCTAGAGATCATATACCAAAATGGTGGATTTGGGGTTTCTGGTTTTCTCCTTTGATGTATGCTCAAAATGCTGCTTCTGTAAATGAATTCCTTGGACACTCCTGGAACACGGGACATGGGTATGAGACCGGTCTTTCATTAGGTCAGTCATTACTAAAAGCACGCAGTTTGTTTCCAGAGAGATATTGGTATTGGATTGGTGCTGGTGCTTTGCTTGGCTATACAGTTTTATTCAATTTACTATTCACATTCTTCCTTGCTTACCTCAACCCCTTGGGGAAGCAACAAGCTGTTGTGTCTAAGGAAGAGTTGGAAGAGAGAGAAAGGAGAAGAAAAGGAGAAAGTGTTGTTATTGAGCTGAGACAGTACTTGAATCATTCACAGTCACTTAATGGAAAATACTTTAAGCAGAGAGGAATGGTCCTCCCATTTCAGCCACTTTCCATGTCTTTCAGCAATATCAATTACTACGTTGATGTCCCTCTGGAATTGAAGCAGCAGGGAATTCAAGAAGAGAGATTGCAGCTCTTGGTTAATGTCACTGGAGCATTTAGGCCTGGGGTGCTTACAGCATTGGTTGGAGTAAGTGGTGCTGGGAAAACCACCCTCATGGACGTATTGGCTGGTCGAAAAACTGGTGGGGTTATAGAAGGAAGCATACATATATCTGGTCATCCAAAAAGACAAGAAACTTTTGCAAGAATTTCGGGTTACTGTGAGCAATCTGATATTCACTCCCCCTGCTTGACTGTAGTTGAATCGCTTCTGTTCTCTGTTTGGCTTCGCTTGCCATCAGACGTTGACTTGGATACACGGAAGGCTTTTGTAGAGGAGGTGATGGAACTTGTGGAGCTAACTCCATTAAGTGGGGCACTGGTTGGTTTGCCGGGGGTGAATGGCTTGTCAACAGAACAACGAAAAAGGCTAACTATAGCAGTGGAGCTGGTCGCTAACCCATCTATAGTATTTATGGATGAACCCACATCTGGGTTAGATGCAAGGTCTGCAGCCATTGTGATGAGGACTGTGAGGAATATAGTCAATACTGGACGTACTATCGTATGCACAATCCACCAGCCTAGCATAGACATTTTTGAATCCTTTGATGAGCTTCTGTTTTTGAAGCGTGGAGGTCAGCTCATATATGCTGGTCCACTTGGACCCAAGTCTTGTGAACTCATCAAGTACTTTGAGGCAATTGAAGGAGTGCAAAAGATCAGACCTGGATATAATCCTGCTGCATGGATGCTTGATGTTACTTCTTCAACTGAAGAAATCCGTCTGGGGGTGGATTTTGCAGAAATTTACAGAAGATCAAATTTGTTTCAACAAAACAAAGACTTGGTTGAAAACCTAAGCAAGCCGAGTACTAATTCCAAAGAATTAAACTTTCCAACCAAATACTCTCAGACATCATTTGAACAATTTCTCACTTGTCTATGGAAGCAAAATCTGTCTTATTGGCGAAACCCTCAGTACACTGCAGTACGTTTCTTTTACACTGTCATCATCTCCTTGATGCTAGGAACAATTTGCTGGAGATTCGGGGCTAAGAGGGGGACCCAGCAGGACTTATTAAATGCCATGGGATCCATGTATGCAGCAATCCTATTTAGTGGAGTCACCAATGCCACTGCTGTTCAACCTGTTGTTTCTATAGAAAGATTTGTCTCATATCGAGAGAGAGCTGCTGGGATGTACTCGGCGTTACCATTTGCATTTGCTCAGGTTGCAATAGAGTTCCCTTACGTGTTTGCGCAAGCGGTCATCTATTGCACAATTTTCTACTCCACGGCTGCATTTGACTGGACACTTTTAAAGTTTGTTTGGTACTTATTCTTCATGTATTTCACAATGCTATACTTCACCTTTTACGGAATGATGACAACAGCCGTCACACCAAATCATAACGTAGCTGCCATAATTGCTGCTCCATTTTACATGCTATGGAATCTTTTCAGCGGCTTCATGATTCCTCACAAGAGAATTCCCGTATGGTGGAGGTGGTATTACTGGGCAAACCCTGTAGCCTGGAGTCTGTATGGCCTGTTTGTTTCGCAGTATGGTGACGATGACAATCTTTTGACGCTAGCAGATGGAGAAAGAACAATGCCGGTAAGGCAGTTCCTCAAAGTTGGTTTTGGGTACAGGCATGATTTCCTAGGTGTAGCCGGAATTATGGTCGTCGGTTTCTGCATACTTTTCGCAATAATTTTTGCCTATGCAATAAAATCATTCAACTTCCAGAGGAGATGA

Protein Analysis

1422

Amino Acids

161.56

Weight (kDa)

8.45

Isoelectric Point (pI)

42.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 74 - 127 3e-08 ABC-transporter N-terminal
ABC_tran PF00005 152 - 333 1.9e-14 ABC transporter
ABC2_membrane PF01061 489 - 700 1.5e-48 ABC-2 type transporter
PDR_assoc PF08370 712 - 769 5.6e-26 Plant PDR ABC transporter associated
ABC_tran PF00005 849 - 999 7.5e-19 ABC transporter
ABC2_membrane PF01061 1144 - 1358 1.3e-54 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
AatII GACGTC 2 cut(s) 168, 635
Acc16I TGCGCA 1 cut(s) 3768
Acc36I ACCTGC 1 cut(s) 1111
AccB1I GGYRCC 1 cut(s) 1678
AccB7I CCANNNNNTGG 1 cut(s) 2876
AccII CGCG 2 cut(s) 23, 29
AclI AACGTT 1 cut(s) 327
AclWI GGATC 6 cut(s) 1835, 1920, 1933, 1972, 3594, 3607
AcoI YGGCCR 2 cut(s) 598, 1692
AcuI CTGAAG 5 cut(s) 212, 249, 554, 1398, 3300
AcyI GRCGYC 4 cut(s) 38, 165, 553, 632
AfeI AGCGCT 1 cut(s) 245
AfiI CCNNNNNNNGG 8 cut(s) 518, 1183, 1744, 2876, 2996, 2997, 3295, 4008
AflIII ACRYGT 2 cut(s) 21, 3759
AgeI ACCGGT 1 cut(s) 2138
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 5 cut(s) 1763, 2111, 2566, 3232, 4040
AloI GAACNNNNNNTCC 2 cut(s) 8, 40
Alw21I GWGCWC 3 cut(s) 361, 518, 1069
Alw26I GTCTC 6 cut(s) 680, 827, 1106, 2129, 2365, 3685
Alw44I GTGCAC 1 cut(s) 1065
AlwI GGATC 6 cut(s) 1835, 1920, 1933, 1972, 3594, 3607
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 2 cut(s) 239, 1176
Aor51HI AGCGCT 1 cut(s) 245
AoxI GGCC 6 cut(s) 598, 931, 1153, 1692, 2564, 4054
ApaLI GTGCAC 1 cut(s) 1065
AsiGI ACCGGT 1 cut(s) 2138
Asp700I GAANNNNTTC 5 cut(s) 23, 1344, 2772, 3435, 3965
AspA2I CCTAGG 1 cut(s) 4171
AspLEI GCGC 5 cut(s) 73, 88, 246, 887, 3769
AsuC2I CCSGG 4 cut(s) 1009, 1177, 1178, 2898
AsuHPI GGTGA 7 cut(s) 800, 968, 2857, 2914, 3625, 3868, 4088
AsuNHI GCTAGC 1 cut(s) 4099
AvaI CYCGRG 2 cut(s) 239, 1176
AvaII GGWCC 9 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170, 3570
AvrII CCTAGG 1 cut(s) 4171
BaeGI GKGCMC 3 cut(s) 1069, 1633, 2884
BaeI ACNNNNGTAYC 2 cut(s) 882, 915
BalI TGGCCA 2 cut(s) 600, 1694
BamHI GGATCC 2 cut(s) 1925, 3599
BanI GGYRCC 1 cut(s) 1678
BanII GRGCYC 2 cut(s) 518, 1014
BarI GAAGNNNNNNTAC 6 cut(s) 1869, 1901, 2652, 2684, 3833, 3865
BauI CACGAG 1 cut(s) 1062
BbsI GAAGAC 2 cut(s) 560, 1564
Bbv12I GWGCWC 3 cut(s) 361, 518, 1069
BccI CCATC 7 cut(s) 779, 1355, 1604, 2809, 2842, 2974, 4100
BceAI ACGGC 4 cut(s) 51, 434, 3819, 3887
BcgI CGANNNNNNTGC 6 cut(s) 1043, 1077, 2697, 2731, 4195, 4229
BciT130I CCWGG 5 cut(s) 1765, 2113, 2568, 3234, 4042
BciVI GTATCC 3 cut(s) 1829, 1994, 2812
BclI TGATCA 1 cut(s) 1557
BcnI CCSGG 4 cut(s) 1009, 1177, 1178, 2898
BcoDI GTCTC 6 cut(s) 680, 827, 1106, 2129, 2365, 3685
BfaI CTAG 8 cut(s) 740, 749, 1854, 2013, 3090, 3533, 4100, 4172
BfmI CTRYAG 9 cut(s) 951, 1419, 2760, 2940, 2970, 3015, 3492, 3666, 4035
BfoI RGCGCY 3 cut(s) 74, 89, 247
BfuAI ACCTGC 1 cut(s) 1111
BfuI GTATCC 3 cut(s) 1829, 1994, 2812
BglI GCCNNNNNGGC 1 cut(s) 4129
BlnI CCTAGG 1 cut(s) 4171
BmcAI AGTACT 4 cut(s) 1286, 2378, 3197, 3379
Bme1390I CCNGG 9 cut(s) 1009, 1177, 1178, 1765, 2113, 2568, 2898, 3234, 4042
Bme18I GGWCC 9 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170, 3570
BmeT110I CYCGRG 2 cut(s) 239, 1176
BmgBI CACGTC 1 cut(s) 122
BmrFI CCNGG 9 cut(s) 1009, 1177, 1178, 1765, 2113, 2568, 2898, 3234, 4042
BmrI ACTGGG 1 cut(s) 4033
BmsI GCATC 3 cut(s) 2995, 3246, 3519
BmtI GCTAGC 1 cut(s) 4103
BmuI ACTGGG 1 cut(s) 4033
BoxI GACNNNNGTC 1 cut(s) 3175
BpiI GAAGAC 2 cut(s) 560, 1564
BplI GAGNNNNNCTC 2 cut(s) 3132, 3164
BpmI CTGGAG 7 cut(s) 94, 110, 197, 1092, 2574, 3568, 4063
Bpu10I CCTNAGC 3 cut(s) 183, 3365, 3735
BpuEI CTTGAG 2 cut(s) 1451, 1792
BpuMI CCSGG 4 cut(s) 1009, 1177, 1178, 2898
BsaAI YACGTR 1 cut(s) 3760
BsaBI GATNNNNATC 2 cut(s) 789, 4086
BsaHI GRCGYC 4 cut(s) 38, 165, 553, 632
BsaI GGTCTC 2 cut(s) 1106, 2129
BsaWI WCCGGW 1 cut(s) 2138
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 2340, 2370
Bsc4I CCNNNNNNNGG 8 cut(s) 518, 1183, 1744, 2876, 2996, 2997, 3295, 4008
Bse118I RCCGGY 3 cut(s) 1324, 2138, 4123
Bse8I GATNNNNATC 2 cut(s) 789, 4086
BseBI CCWGG 5 cut(s) 1765, 2113, 2568, 3234, 4042
BseGI GGATG 5 cut(s) 1000, 2683, 2989, 3261, 3711
BseJI GATNNNNATC 2 cut(s) 789, 4086
BseLI CCNNNNNNNGG 8 cut(s) 518, 1183, 1744, 2876, 2996, 2997, 3295, 4008
BseMII CTCAG 7 cut(s) 197, 280, 398, 2360, 3431, 3497, 3749
BseRI GAGGAG 2 cut(s) 80, 2855
BseSI GKGCMC 3 cut(s) 1069, 1633, 2884
BseYI CCCAGC 3 cut(s) 2603, 3573, 3701
BsgI GTGCAG 1 cut(s) 1874
Bsh1236I CGCG 2 cut(s) 23, 29
BshFI GGCC 6 cut(s) 600, 933, 1155, 1694, 2566, 4056
BshNI GGYRCC 1 cut(s) 1678
BshTI ACCGGT 1 cut(s) 2138
BsiHKAI GWGCWC 3 cut(s) 361, 518, 1069
BsiHKCI CYCGRG 2 cut(s) 239, 1176
BslFI GGGAC 6 cut(s) 1185, 1206, 1736, 2136, 2474, 3583
BslI CCNNNNNNNGG 8 cut(s) 518, 1183, 1744, 2876, 2996, 2997, 3295, 4008
BsmAI GTCTC 6 cut(s) 680, 827, 1106, 2129, 2365, 3685
BsmFI GGGAC 6 cut(s) 1185, 1206, 1736, 2136, 2474, 3583
BsmI GAATGC 2 cut(s) 1054, 1513
BsnI GGCC 6 cut(s) 600, 933, 1155, 1694, 2566, 4056
Bso31I GGTCTC 2 cut(s) 1106, 2129
BsoBI CYCGRG 2 cut(s) 239, 1176
Bsp1286I GDGCHC 6 cut(s) 361, 518, 1014, 1069, 1633, 2884
Bsp19I CCATGG 3 cut(s) 601, 1644, 3594
Bsp68I TCGCGA 1 cut(s) 29
BspANI GGCC 6 cut(s) 600, 933, 1155, 1694, 2566, 4056
BspCNI CTCAG 7 cut(s) 196, 279, 399, 2361, 3430, 3496, 3748
BspFNI CGCG 2 cut(s) 23, 29
BspHI TCATGA 3 cut(s) 804, 858, 3978
BspMAI CTGCAG 3 cut(s) 1423, 3019, 3496
BspMI ACCTGC 1 cut(s) 1111
BspOI GCTAGC 1 cut(s) 4103
BspPI GGATC 6 cut(s) 1835, 1920, 1933, 1972, 3594, 3607
BspT107I GGYRCC 1 cut(s) 1678
BspTNI GGTCTC 2 cut(s) 1106, 2129
BsrFI RCCGGY 3 cut(s) 1324, 2138, 4123
BssAI RCCGGY 3 cut(s) 1324, 2138, 4123
BssNI GRCGYC 4 cut(s) 38, 165, 553, 632
BssSI CACGAG 1 cut(s) 1062
BssT1I CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
Bst2BI CACGAG 1 cut(s) 1062
Bst2UI CCWGG 5 cut(s) 1765, 2113, 2568, 3234, 4042
Bst6I CTCTTC 5 cut(s) 1374, 1445, 2310, 2319, 2517
BstACI GRCGYC 4 cut(s) 38, 165, 553, 632
BstAPI GCANNNNNTGC 1 cut(s) 3014
BstBAI YACGTR 1 cut(s) 3760
BstC8I GCNNGC 6 cut(s) 173, 2173, 2798, 3371, 3771, 4101
BstDSI CCRYGG 4 cut(s) 601, 1644, 3594, 3801
BstF5I GGATG 5 cut(s) 1000, 2683, 2989, 3261, 3711
BstFNI CGCG 2 cut(s) 23, 29
BstH2I RGCGCY 3 cut(s) 74, 89, 247
BstHHI GCGC 5 cut(s) 73, 88, 246, 887, 3769
BstMAI GTCTC 6 cut(s) 680, 827, 1106, 2129, 2365, 3685
BstNI CCWGG 5 cut(s) 1765, 2113, 2568, 3234, 4042
BstNSI RCATGY 1 cut(s) 3955
BstPAI GACNNNNGTC 1 cut(s) 3175
BstSCI CCNGG 9 cut(s) 1007, 1175, 1176, 1763, 2111, 2566, 2896, 3232, 4040
BstSFI CTRYAG 9 cut(s) 951, 1419, 2760, 2940, 2970, 3015, 3492, 3666, 4035
BstSLI GKGCMC 3 cut(s) 1069, 1633, 2884
BstUI CGCG 2 cut(s) 23, 29
BstV2I GAAGAC 2 cut(s) 560, 1564
BstX2I RGATCY 3 cut(s) 1925, 1964, 3599
BstXI CCANNNNNNTGG 2 cut(s) 2034, 2620
BstYI RGATCY 3 cut(s) 1925, 1964, 3599
BsuI GTATCC 3 cut(s) 1829, 1994, 2812
BsuRI GGCC 6 cut(s) 600, 933, 1155, 1694, 2566, 4056
BtgI CCRYGG 4 cut(s) 601, 1644, 3594, 3801
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 5 cut(s) 1000, 2683, 2989, 3261, 3711
BtsI GCAGTG 4 cut(s) 359, 2952, 3489, 3642
BtuMI TCGCGA 1 cut(s) 29
BveI ACCTGC 1 cut(s) 1111
Cac8I GCNNGC 6 cut(s) 173, 2173, 2798, 3371, 3771, 4101
CaiI CAGNNNCTG 1 cut(s) 190
CciI TCATGA 3 cut(s) 804, 858, 3978
CfoI GCGC 5 cut(s) 73, 88, 246, 887, 3769
Cfr10I RCCGGY 3 cut(s) 1324, 2138, 4123
Cfr9I CCCGGG 1 cut(s) 1176
CseI GACGC 3 cut(s) 27, 561, 4105
CspAI ACCGGT 1 cut(s) 2138
CspCI CAANNNNNGTGG 6 cut(s) 448, 483, 825, 860, 1671, 1706
DraI TTTAAA 1 cut(s) 3828
EaeI YGGCCR 2 cut(s) 598, 1692
Eam1104I CTCTTC 5 cut(s) 1374, 1445, 2310, 2319, 2517
EarI CTCTTC 5 cut(s) 1374, 1445, 2310, 2319, 2517
EciI GGCGGA 2 cut(s) 29, 683
Ecl136II GAGCTC 1 cut(s) 516
Eco130I CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
Eco147I AGGCCT 1 cut(s) 2566
Eco24I GRGCYC 2 cut(s) 518, 1014
Eco31I GGTCTC 2 cut(s) 1106, 2129
Eco47I GGWCC 9 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170, 3570
Eco47III AGCGCT 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 516
Eco57I CTGAAG 5 cut(s) 212, 249, 554, 1398, 3300
Eco88I CYCGRG 2 cut(s) 239, 1176
EcoICRI GAGCTC 1 cut(s) 516
EcoO109I RGGNCCY 2 cut(s) 1172, 3570
EcoRI GAATTC 3 cut(s) 2096, 2514, 3996
EcoRII CCWGG 5 cut(s) 1763, 2111, 2566, 3232, 4040
EcoT14I CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
EcoT22I ATGCAT 1 cut(s) 1513
EcoT38I GRGCYC 2 cut(s) 518, 1014
ErhI CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
FalI AAGNNNNNCTT 6 cut(s) 478, 510, 1173, 1205, 1460, 1492
FaqI GGGAC 6 cut(s) 1185, 1206, 1736, 2136, 2474, 3583
FauI CCCGC 2 cut(s) 1232, 1852
FauNDI CATATG 1 cut(s) 881
FbaI TGATCA 1 cut(s) 1557
FokI GGATG 5 cut(s) 1007, 2670, 2996, 3268, 3718
FriOI GRGCYC 2 cut(s) 518, 1014
FspBI CTAG 8 cut(s) 740, 749, 1854, 2013, 3090, 3533, 4100, 4172
FspI TGCGCA 1 cut(s) 3768
GlaI GCGC 5 cut(s) 72, 87, 245, 886, 3768
GsaI CCCAGC 3 cut(s) 2607, 3577, 3705
GsuI CTGGAG 7 cut(s) 94, 110, 197, 1092, 2574, 3568, 4063
HaeII RGCGCY 3 cut(s) 74, 89, 247
HaeIII GGCC 6 cut(s) 600, 933, 1155, 1694, 2566, 4056
HgaI GACGC 3 cut(s) 27, 561, 4105
HhaI GCGC 5 cut(s) 73, 88, 246, 887, 3769
Hin1I GRCGYC 4 cut(s) 38, 165, 553, 632
Hin6I GCGC 5 cut(s) 71, 86, 244, 885, 3767
HinP1I GCGC 5 cut(s) 71, 86, 244, 885, 3767
HincII GTYRAC 5 cut(s) 163, 217, 650, 2812, 2916
HindII GTYRAC 5 cut(s) 163, 217, 650, 2812, 2916
HindIII AAGCTT 4 cut(s) 333, 1341, 1474, 1697
HphI GGTGA 7 cut(s) 800, 968, 2857, 2914, 3625, 3868, 4088
Hpy99I CGWCG 4 cut(s) 43, 123, 637, 4199
Hsp92I GRCGYC 4 cut(s) 38, 165, 553, 632
HspAI GCGC 5 cut(s) 71, 86, 244, 885, 3767
KflI GGGWCCC 2 cut(s) 1172, 3570
Ksp22I TGATCA 1 cut(s) 1557
LmnI GCTCC 9 cut(s) 187, 429, 521, 1111, 1459, 2555, 2860, 2950, 3946
LweI GCATC 3 cut(s) 2995, 3246, 3519
MaeI CTAG 8 cut(s) 740, 749, 1854, 2013, 3090, 3533, 4100, 4172
MaeIII GTNAC 8 cut(s) 2394, 2548, 2720, 3265, 3631, 3717, 3904, 4076
MfeI CAATTG 2 cut(s) 1602, 3207
MflI RGATCY 3 cut(s) 1925, 1964, 3599
MhlI GDGCHC 6 cut(s) 361, 518, 1014, 1069, 1633, 2884
MlsI TGGCCA 2 cut(s) 600, 1694
MluI ACGCGT 1 cut(s) 21
MluNI TGGCCA 2 cut(s) 600, 1694
MlyI GAGTC 6 cut(s) 123, 258, 682, 1805, 3639, 4054
MmeI TCCRAC 6 cut(s) 142, 700, 855, 2301, 2569, 3428
Mox20I TGGCCA 2 cut(s) 600, 1694
Mph1103I ATGCAT 1 cut(s) 1513
MroXI GAANNNNTTC 5 cut(s) 23, 1344, 2772, 3435, 3965
MscI TGGCCA 2 cut(s) 600, 1694
MslI CAYNNNNRTG 3 cut(s) 2130, 2618, 3026
Msp20I TGGCCA 2 cut(s) 600, 1694
MspA1I CMGCKG 1 cut(s) 3972
MspR9I CCNGG 9 cut(s) 1009, 1177, 1178, 1765, 2113, 2568, 2898, 3234, 4042
MunI CAATTG 2 cut(s) 1602, 3207
Mva1269I GAATGC 2 cut(s) 1054, 1513
MvaI CCWGG 5 cut(s) 1765, 2113, 2568, 3234, 4042
MvnI CGCG 2 cut(s) 23, 29
NciI CCSGG 4 cut(s) 1009, 1177, 1178, 2898
NcoI CCATGG 3 cut(s) 601, 1644, 3594
NdeI CATATG 1 cut(s) 881
NheI GCTAGC 1 cut(s) 4099
NmeAIII GCCGAG 2 cut(s) 3399, 3692
NmuCI GTSAC 5 cut(s) 2394, 2548, 3631, 3904, 4076
NruI TCGCGA 1 cut(s) 29
NsbI TGCGCA 1 cut(s) 3768
NsiI ATGCAT 1 cut(s) 1513
NspI RCATGY 1 cut(s) 3955
PagI TCATGA 3 cut(s) 804, 858, 3978
PceI AGGCCT 1 cut(s) 2566
PctI GAATGC 2 cut(s) 1054, 1513
PdmI GAANNNNTTC 5 cut(s) 23, 1344, 2772, 3435, 3965
PfeI GAWTC 8 cut(s) 723, 1073, 2383, 2768, 3107, 3552, 3961, 3982
PflFI GACNNNGTC 2 cut(s) 37, 708
PflMI CCANNNNNTGG 1 cut(s) 2876
PfoI TCCNGGA 1 cut(s) 2111
PinAI ACCGGT 1 cut(s) 2138
PleI GAGTC 6 cut(s) 122, 258, 682, 1804, 3638, 4053
PpsI GAGTC 6 cut(s) 122, 258, 682, 1804, 3638, 4053
Ppu21I YACGTR 1 cut(s) 3760
PpuMI RGGWCCY 2 cut(s) 1172, 3570
PshAI GACNNNNGTC 1 cut(s) 3175
PsiI TTATAA 1 cut(s) 476
Psp124BI GAGCTC 1 cut(s) 518
Psp1406I AACGTT 1 cut(s) 327
Psp5II RGGWCCY 2 cut(s) 1172, 3570
Psp6I CCWGG 5 cut(s) 1763, 2111, 2566, 3232, 4040
PspFI CCCAGC 3 cut(s) 2603, 3573, 3701
PspGI CCWGG 5 cut(s) 1763, 2111, 2566, 3232, 4040
PspPPI RGGWCCY 2 cut(s) 1172, 3570
PstI CTGCAG 3 cut(s) 1423, 3019, 3496
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 3 cut(s) 1925, 1964, 3599
PsyI GACNNNGTC 2 cut(s) 37, 708
RruI TCGCGA 1 cut(s) 29
RseI CAYNNNNRTG 3 cut(s) 2130, 2618, 3026
SacI GAGCTC 1 cut(s) 518
ScaI AGTACT 4 cut(s) 1286, 2378, 3197, 3379
SchI GAGTC 6 cut(s) 123, 258, 682, 1805, 3639, 4054
ScrFI CCNGG 9 cut(s) 1009, 1177, 1178, 1765, 2113, 2568, 2898, 3234, 4042
SduI GDGCHC 6 cut(s) 361, 518, 1014, 1069, 1633, 2884
SfaNI GCATC 3 cut(s) 2995, 3246, 3519
SfcI CTRYAG 9 cut(s) 951, 1419, 2760, 2940, 2970, 3015, 3492, 3666, 4035
SinI GGWCC 9 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170, 3570
SmaI CCCGGG 1 cut(s) 1178
SmiMI CAYNNNNRTG 3 cut(s) 2130, 2618, 3026
SmlI CTYRAG 2 cut(s) 1466, 1771
SmoI CTYRAG 2 cut(s) 1466, 1771
SseBI AGGCCT 1 cut(s) 2566
SspMI CTAG 8 cut(s) 740, 749, 1854, 2013, 3090, 3533, 4100, 4172
SstI GAGCTC 1 cut(s) 518
StuI AGGCCT 1 cut(s) 2566
StyD4I CCNGG 9 cut(s) 1007, 1175, 1176, 1763, 2111, 2566, 2896, 3232, 4040
StyI CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
TaqI TCGA 5 cut(s) 303, 1014, 1936, 2638, 3688
TaqII GACCGA 1 cut(s) 410
TatI WGTACW 8 cut(s) 852, 1284, 1635, 2376, 3195, 3377, 3486, 3708
TauI GCSGC 6 cut(s) 86, 625, 668, 946, 1187, 3975
TfiI GAWTC 8 cut(s) 723, 1073, 2383, 2768, 3107, 3552, 3961, 3982
TseFI GTSAC 5 cut(s) 2394, 2548, 3631, 3904, 4076
Tsp45I GTSAC 5 cut(s) 2394, 2548, 3631, 3904, 4076
TspGWI ACGGA 4 cut(s) 1688, 2839, 3278, 3900
TspMI CCCGGG 1 cut(s) 1176
Tth111I GACNNNGTC 2 cut(s) 37, 708
Van91I CCANNNNNTGG 1 cut(s) 2876
VneI GTGCAC 1 cut(s) 1065
VpaK11BI GGWCC 9 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170, 3570
XbaI TCTAGA 2 cut(s) 1853, 2012
XceI RCATGY 1 cut(s) 3955
XcmI CCANNNNNNNNNTGG 1 cut(s) 2193
XmaI CCCGGG 1 cut(s) 1176
XmaJI CCTAGG 1 cut(s) 4171
XmnI GAANNNNTTC 5 cut(s) 23, 1344, 2772, 3435, 3965
XspI CTAG 8 cut(s) 740, 749, 1854, 2013, 3090, 3533, 4100, 4172
ZraI GACGTC 2 cut(s) 166, 633
ZrmI AGTACT 4 cut(s) 1286, 2378, 3197, 3379
Zsp2I ATGCAT 1 cut(s) 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.