Rroxscaffold_2G00151650
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008072
Physical Location & Seq
Forward (+)
88857007 .. 88864503
7497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00151650.1

Sequence Viewer

Length: 4044 bp
ATGTGGAACTCGGCGGAGAACGCGTTTGCGAGGACGGCGTCGTTTCGGGTGGAGGGGGAGGACGAGGAGGCGCTGCGGTGGGCGGCGCTGGAGAGGCTGCCGACATACTCCAGAGTCCGACGTGGCATTTTCAAGAACATTGTGGGAGATACCAAGGAGGTTGACGTCAGCGAGCTGGAGGCTCAGGAGCTGAAGCTACTGCTTGATCGGCTTGTTAACTCGGCTGAAGATGACCCCGAGCACTTCTTTGAGAAAATGCGACTCAGATTTAACGCAGTAGATTTGGAGTTTCCGAAGATCGAGGTGCGGTTTCAGAATTTGAACGTTGAAGCTTTCGTCCATGTGGGAAGCAGAGCACTGCCAACTATTCCGAATTTCGTATTCAATATGACCGAGGCACTTCTGAGGCAATTACGGCTATTAAGGAGCAAGAGAAGCAAATTAACGATTTTAGACAACATTAGTGGGATTATAAGACCTTCAAGATTGACGTTGTTATTGGGTCCACCGAGCTCCGGCAAGACGACGCTACTATTGGCTCTCGCCGGACGCCTTGGAACTGGTCTTCAGATGTCAGGTAGCACAACATACAATGGCCATGGCTTAAACGAGTTTGTGCCGCAGAGGACGTCGGCTTATGTCAGTCAACAGGACTGGTTTGCGGCGGAGATGACTGTAAGGGAGACTCTTGACTTTGCAGGACGGTGTCAAGGTGTTGGATTCAAATATGATATGCTAGTAGAACTAGCAAGAAGGGAAAAGATTGCGGGGATAAAACCCGATGGTGATCTTGATATATTCATGAAGTCATTAGCTCTTGGAGAGCAAGAGACAAGCCTTGTGGTAGAGTACATCATGAAGATTTTAGGGTTGGACATATGCGCTGATACGTTGGTAGGAGATGAAATGCTTAAGGGTATATCAGGGGGCCAAAAGAAGCGGCTTACTACAGGTGAATTACTAGTAGGTCCAGCAAGAGTGTTGTTCATGGACGAAATATCAACGGGGCTCGATAGTTCCACTACTTATCAAATCATCAAGTATCTTAGGCATTCAACTCGTGCACTTGATTCCACCACTGTTATATCTCTGCTTCAACCTGCTCCAGAGACCTACGAGTTGTTTGATGATGTTATACTTTTGTGTGAGGGCCAGATAGTGTTTCAGGGACCCCGGGAAGCTGCTGTTGATTTCTTTTCATACATGGGGTTTAGATGTCCCCGCCGAAAGAATGTGGCAGACTTCTTGCAAGAAGTTATATCAAAGAAGGACCAAGAGCAGTACTGGTCCAATCCTGATCTTCCATACCGCTATATACCACCAGCAAAGTTTGTTGAAGCTTTTCCTTCATTTCAAGATGGGAAGAATTTGTCTGAAGAGTTGAAAGTTCCTTTTGATAAACGCTATAACCACCCTGCAGCTTTGGCGACTTCTCTCTATGGCATGAAGAGGAAGGAGCTTCTCAAGACAAGCTTTAACTGGCAAGTGCTACTGATGAAGCGGAATGCATTTATTTATATTTTCAAATTTGTTCAGCTTCTTTTGGTTGCTTTGATCACAATGAGTGTCTTCTGCCGGACAAAAATGCACCATGATACAATTGATGATGGAGGCTTATATCTCGGGGCACTATACTTTTCCATGGTTATTATTCTTTTTAATGGCTTTACGGAGGTGCCAATGTTGGTGGCCAAGCTTCCAGTGCTTTACAAGCATAGGGACTTACACTTCTACCCAAGTTGGGTCTATACTCTTCCTTCCTGGCTCTTGAGTATTCCAAATTCACTCATAGAGTCTGGACTTTGGGTGGCAATCACATACTACGTCATTGGATATGATCCCGCAATTTCTAGATTTTTTGGGCAGTTCTTGGTATATTTCCTTCTGCACCAGATGTCCACAGCTCTCTTTCGTCTTATGGGATCCTTGGGTCGAAATATGATAGTTGCCAACACCTTTGGATCTTTTGCCATGTTGGTTGTAATGGCTCTTGGAGGATACATCATCTCTAGAGATCATATACCAAAATGGTGGATTTGGGGTTTCTGGTTTTCTCCTTTGATGTATGCTCAAAATGCTGCTTCTGTAAATGAATTCCTTGGACACTCCTGGAACACGGGACATGGGTATGGGACCGGTCTTTCATTAGGTCAATCATTACTAAAAGCACGCAGTTTGTTTCCAGAGAGATACTGGTATTGGATTGGTGCTGGTGCTTTGCTTGGCTATACCGTTTTATTCAATTTACTATTCACATTCTTCCTTGCCTACCTCAACCCCTTGGGGAAGCAACAAGCTGTTGTGTCTAAGGAAGAGCTGGAAGAGAGAGAAAGGAGAAGAAAAGGAGAAAGTGTTGTTATTGAGCTGAGACAGTACTTGAATCATTCAGAGTCACTTAATGGAAAATACTTTAAGCAGAGAGGAATGGTCCTCCCATTTCAGCCACTTTCCATGTCTTTCAGCAATATCAATTACTATGTTGATGTCCCTCTGGCCGGGGTGCTTACAGCATTGGTTGGAGTAAGTGGTGCTGGTAAAACCACCCTCATGGACGTATTGGCTGGTCGAAAAACTGGTGGGGTTATAGAAGGAAGCATACATATATCTGGTCATCCAAAAAGACAAGAAACTTTTGCAAGAATTTCGGGTTACTGTGAGCAATCTGATATTCACTCCCCCTGCTTGACTGTTGTTGAATCGCTTCTGTTCTCTGTTTGGCTTCGCTTGTCATCAGATGTTGACTTGGATACACGGAAGGCTTTTGTAGAGGAGGTGATGGAACTTGTGGAGCTCACTCCATTACGTGGGGCACTGGTTGGTTTACCGGGGGTGAATGGCTTGTCAACAGAACAACGAAAAAGGCTAACTATAGCAGTGGAGCTGGTCGCTAACCCATCTATAGTATTCATGGATGAACCCACATCTGGGTTAGATGCGAGGTCTGCAGCCATTGTGATGAGGACTGTGAGGAATATAGTCAATACTGGACGTACTATTGTATGCACAATCCACCAGCCTAGCATAGACATTTTTGAATCCTTTGATGAGCTTCTGTTTTTGAAGCGTGGAGGTCAGCTCATATATGCTGGTCCACTTGGACCCAACTCTTGTGAACTCATCAAGTACTTTGAGGCAATTGAAGGAGTGCAAAAGATCAGGCCTGGATATAATCCTGCAGCATGGATGCTTGATGTTACTTCTTCAACTGAAGAAATCCGTCTGGGGGTGGATTTTGCAGAAATTTACAGAAGATCAAATTTGTTTCAACAAAACAAAGACTTGGTTGAAAACCTAAGCAAGCCGAGTACTAATTCCAAAGAATTAAACTTTCCAACCAAATACTCTCAGACATCATTTGAACAATTTCTCACTTGTCTATGGAAGCAAAATCTGTCTTATTGGCGAAACCCTCAGTACACTGCAGTTCGTTTCTTTTATACTGTCATCATCGCCTTGATGCTAGGAACAATTTGTTGGAGATTCGGGGCTAAGAGGGAGACCCAGCAGGACTTATTAAATGCCATGGGATCCTTGTATGCAGCAATCCTATTTAGTGGAGTCACAAATGCCACTGCTGTTCAACCTGTTGTTTCTATAGAAAGATTTGTCTCATATCGAGAGAGAGCTGCTGGGATGTACTCTGCGTTACCATTTGCATTTGCTCAGGTTGCAATAGAGTTCCCTTATGTGTTTGCGCAGGCGGTCATCTACTGCACAATTTTCTACTCCACGGCTGCATTTGACTGGACACTTTTAAAGTTTGTTTGGTACTTATTCTTCATGTATTTCACAATGCTATACTTCACCTTTTACGGAATGATGACAACAGCCGTCACACCAAATCATAACGTAGCTGCCATAATTGCTGCTCCATTTTACATGCTATGGAATCTTTTCAGCGGCTTCATGATTCCTCACAAGAGAATTCCCGTATGGTGGAGATGGTATTACTGGGCAAACCCTGTAGCCTGGAGTCTGTATGGCCTGTTTGTTTCGCAGTACGGTGATGATGACAGTCTTTTGACGCTAGCAGATGGAGAATGA

Protein Analysis

1347

Amino Acids

152.98

Weight (kDa)

7.55

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 73 - 126 9.7e-08 ABC-transporter N-terminal
ABC_tran PF00005 151 - 332 1.7e-14 ABC transporter
ABC2_membrane PF01061 488 - 699 2e-48 ABC-2 type transporter
PDR_assoc PF08370 713 - 768 1e-25 Plant PDR ABC transporter associated
ABC_tran PF00005 833 - 975 9.8e-18 ABC transporter
ABC2_membrane PF01061 1120 - 1334 4.1e-55 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 473
AatII GACGTC 2 cut(s) 168, 632
Acc16I TGCGCA 1 cut(s) 3696
Acc36I ACCTGC 1 cut(s) 1108
AccB1I GGYRCC 1 cut(s) 1675
AccB7I CCANNNNNTGG 1 cut(s) 2804
AccII CGCG 1 cut(s) 23
AclI AACGTT 1 cut(s) 324
AclWI GGATC 6 cut(s) 1832, 1917, 1930, 1969, 3522, 3535
AcoI YGGCCR 3 cut(s) 595, 1689, 2493
AcuI CTGAAG 5 cut(s) 212, 246, 551, 1395, 3228
AcyI GRCGYC 4 cut(s) 38, 165, 550, 629
AfiI CCNNNNNNNGG 8 cut(s) 515, 1741, 2495, 2804, 2924, 2925, 3223, 3936
AflII CTTAAG 1 cut(s) 911
AflIII ACRYGT 1 cut(s) 21
AgeI ACCGGT 1 cut(s) 2135
AhlI ACTAGT 1 cut(s) 961
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 4 cut(s) 1760, 2108, 3160, 3968
AjuI GAANNNNNNNTTGG 2 cut(s) 3457, 3489
Alw21I GWGCWC 5 cut(s) 243, 358, 515, 1066, 2793
Alw26I GTCTC 6 cut(s) 677, 824, 1103, 2362, 3491, 3613
Alw44I GTGCAC 1 cut(s) 1062
AlwI GGATC 6 cut(s) 1832, 1917, 1930, 1969, 3522, 3535
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 3 cut(s) 236, 1173, 1622
AoxI GGCC 7 cut(s) 595, 928, 1150, 1689, 2493, 3158, 3982
ApaLI GTGCAC 1 cut(s) 1062
AsiGI ACCGGT 1 cut(s) 2135
Asp700I GAANNNNTTC 4 cut(s) 1341, 2700, 3363, 3893
AspLEI GCGC 4 cut(s) 73, 88, 884, 3697
AsuC2I CCSGG 4 cut(s) 1174, 1175, 2497, 2826
AsuHPI GGTGA 6 cut(s) 797, 965, 2785, 2842, 3796, 4016
AsuNHI GCTAGC 1 cut(s) 4027
AvaI CYCGRG 3 cut(s) 236, 1173, 1622
AvaII GGWCC 9 cut(s) 503, 968, 1169, 1270, 1287, 2133, 2428, 3089, 3098
BaeGI GKGCMC 3 cut(s) 1066, 1630, 2812
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BalI TGGCCA 2 cut(s) 597, 1691
BamHI GGATCC 2 cut(s) 1922, 3527
BanI GGYRCC 1 cut(s) 1675
BanII GRGCYC 3 cut(s) 515, 1011, 2793
BarI GAAGNNNNNNTAC 6 cut(s) 1866, 1898, 2580, 2612, 3761, 3793
BauI CACGAG 1 cut(s) 1059
BbsI GAAGAC 2 cut(s) 557, 1561
Bbv12I GWGCWC 5 cut(s) 243, 358, 515, 1066, 2793
BccI CCATC 7 cut(s) 776, 1352, 1601, 2770, 2902, 3936, 4028
BceAI ACGGC 4 cut(s) 51, 431, 3747, 3815
BcgI CGANNNNNNTGC 4 cut(s) 1040, 1074, 2625, 2659
BciT130I CCWGG 4 cut(s) 1762, 2110, 3162, 3970
BciVI GTATCC 2 cut(s) 1991, 2740
BclI TGATCA 1 cut(s) 1554
BcnI CCSGG 4 cut(s) 1174, 1175, 2497, 2826
BcoDI GTCTC 6 cut(s) 677, 824, 1103, 2362, 3491, 3613
BcuI ACTAGT 1 cut(s) 961
BfaI CTAG 8 cut(s) 737, 746, 962, 1851, 2010, 3018, 3461, 4028
BfmI CTRYAG 9 cut(s) 948, 1416, 2868, 2898, 2943, 3174, 3420, 3594, 3963
BfoI RGCGCY 2 cut(s) 74, 89
BfrI CTTAAG 1 cut(s) 911
BfuAI ACCTGC 1 cut(s) 1108
BfuI GTATCC 2 cut(s) 1991, 2740
BmcAI AGTACT 4 cut(s) 1283, 2375, 3125, 3307
Bme1390I CCNGG 8 cut(s) 1174, 1175, 1762, 2110, 2497, 2826, 3162, 3970
Bme18I GGWCC 9 cut(s) 503, 968, 1169, 1270, 1287, 2133, 2428, 3089, 3098
BmeT110I CYCGRG 3 cut(s) 236, 1173, 1622
BmgBI CACGTC 1 cut(s) 122
BmiI GGNNCC 9 cut(s) 504, 929, 1170, 1171, 1677, 1924, 2134, 3100, 3529
BmrFI CCNGG 8 cut(s) 1174, 1175, 1762, 2110, 2497, 2826, 3162, 3970
BmrI ACTGGG 1 cut(s) 3961
BmsI GCATC 3 cut(s) 2923, 3174, 3447
BmtI GCTAGC 1 cut(s) 4031
BmuI ACTGGG 1 cut(s) 3961
BpiI GAAGAC 2 cut(s) 557, 1561
BplI GAGNNNNNCTC 2 cut(s) 3060, 3092
BpmI CTGGAG 5 cut(s) 94, 110, 197, 1089, 3991
Bpu10I CCTNAGC 3 cut(s) 183, 3293, 3663
BpuEI CTTGAG 2 cut(s) 1448, 1789
BpuMI CCSGG 4 cut(s) 1174, 1175, 2497, 2826
BsaAI YACGTR 1 cut(s) 2804
BsaBI GATNNNNATC 1 cut(s) 786
BsaHI GRCGYC 4 cut(s) 38, 165, 550, 629
BsaI GGTCTC 2 cut(s) 1103, 3491
BsaWI WCCGGW 1 cut(s) 2135
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 2337, 2367
Bsc4I CCNNNNNNNGG 8 cut(s) 515, 1741, 2495, 2804, 2924, 2925, 3223, 3936
Bse118I RCCGGY 1 cut(s) 2135
Bse8I GATNNNNATC 1 cut(s) 786
BseBI CCWGG 4 cut(s) 1762, 2110, 3162, 3970
BseGI GGATG 4 cut(s) 2611, 2917, 3189, 3639
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 8 cut(s) 515, 1741, 2495, 2804, 2924, 2925, 3223, 3936
BseMII CTCAG 7 cut(s) 197, 277, 395, 2357, 3359, 3425, 3677
BseRI GAGGAG 2 cut(s) 80, 2783
BseSI GKGCMC 3 cut(s) 1066, 1630, 2812
BseYI CCCAGC 2 cut(s) 3501, 3629
BsgI GTGCAG 2 cut(s) 1871, 3697
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 7 cut(s) 597, 930, 1152, 1691, 2495, 3160, 3984
BshNI GGYRCC 1 cut(s) 1675
BshTI ACCGGT 1 cut(s) 2135
BsiHKAI GWGCWC 5 cut(s) 243, 358, 515, 1066, 2793
BsiHKCI CYCGRG 3 cut(s) 236, 1173, 1622
BsiSI CCGG 7 cut(s) 516, 546, 1174, 1576, 2136, 2496, 2825
BslFI GGGAC 6 cut(s) 1182, 1203, 1733, 2133, 2146, 2471
BslI CCNNNNNNNGG 8 cut(s) 515, 1741, 2495, 2804, 2924, 2925, 3223, 3936
BsmAI GTCTC 6 cut(s) 677, 824, 1103, 2362, 3491, 3613
BsmFI GGGAC 6 cut(s) 1182, 1203, 1733, 2133, 2146, 2471
BsmI GAATGC 2 cut(s) 1051, 1510
BsnI GGCC 7 cut(s) 597, 930, 1152, 1691, 2495, 3160, 3984
Bso31I GGTCTC 2 cut(s) 1103, 3491
BsoBI CYCGRG 3 cut(s) 236, 1173, 1622
Bsp1286I GDGCHC 8 cut(s) 243, 358, 515, 1011, 1066, 1630, 2793, 2812
Bsp19I CCATGG 3 cut(s) 598, 1641, 3522
BspANI GGCC 7 cut(s) 597, 930, 1152, 1691, 2495, 3160, 3984
BspCNI CTCAG 7 cut(s) 196, 276, 396, 2358, 3358, 3424, 3676
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 3 cut(s) 801, 855, 3906
BspLI GGNNCC 9 cut(s) 504, 929, 1170, 1171, 1677, 1924, 2134, 3100, 3529
BspMAI CTGCAG 4 cut(s) 1420, 2947, 3178, 3424
BspMI ACCTGC 1 cut(s) 1108
BspOI GCTAGC 1 cut(s) 4031
BspPI GGATC 6 cut(s) 1832, 1917, 1930, 1969, 3522, 3535
BspQI GCTCTTC 1 cut(s) 2307
BspT107I GGYRCC 1 cut(s) 1675
BspTI CTTAAG 1 cut(s) 911
BspTNI GGTCTC 2 cut(s) 1103, 3491
BsrFI RCCGGY 1 cut(s) 2135
BssAI RCCGGY 1 cut(s) 2135
BssNI GRCGYC 4 cut(s) 38, 165, 550, 629
BssSI CACGAG 1 cut(s) 1059
BssT1I CCWWGG 8 cut(s) 153, 553, 598, 1641, 1926, 2098, 2280, 3522
Bst2BI CACGAG 1 cut(s) 1059
Bst2UI CCWGG 4 cut(s) 1762, 2110, 3162, 3970
Bst6I CTCTTC 5 cut(s) 1371, 1442, 1758, 2307, 2316
BstACI GRCGYC 4 cut(s) 38, 165, 550, 629
BstAFI CTTAAG 1 cut(s) 911
BstBAI YACGTR 1 cut(s) 2804
BstC8I GCNNGC 5 cut(s) 173, 2170, 3299, 3699, 4029
BstDSI CCRYGG 4 cut(s) 598, 1641, 3522, 3729
BstF5I GGATG 4 cut(s) 2611, 2917, 3189, 3639
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 2 cut(s) 74, 89
BstHHI GCGC 4 cut(s) 73, 88, 884, 3697
BstMAI GTCTC 6 cut(s) 677, 824, 1103, 2362, 3491, 3613
BstNI CCWGG 4 cut(s) 1762, 2110, 3162, 3970
BstNSI RCATGY 1 cut(s) 3883
BstSCI CCNGG 8 cut(s) 1172, 1173, 1760, 2108, 2495, 2824, 3160, 3968
BstSFI CTRYAG 9 cut(s) 948, 1416, 2868, 2898, 2943, 3174, 3420, 3594, 3963
BstSLI GKGCMC 3 cut(s) 1066, 1630, 2812
BstUI CGCG 1 cut(s) 23
BstV2I GAAGAC 2 cut(s) 557, 1561
BstX2I RGATCY 3 cut(s) 1922, 1961, 3527
BstXI CCANNNNNNTGG 2 cut(s) 2031, 2548
BstYI RGATCY 3 cut(s) 1922, 1961, 3527
BsuI GTATCC 2 cut(s) 1991, 2740
BsuRI GGCC 7 cut(s) 597, 930, 1152, 1691, 2495, 3160, 3984
BtgI CCRYGG 4 cut(s) 598, 1641, 3522, 3729
BtgZI GCGATG 1 cut(s) 3433
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 4 cut(s) 2611, 2917, 3189, 3639
BtsI GCAGTG 4 cut(s) 356, 2880, 3417, 3570
BtsIMutI CAGTG 7 cut(s) 356, 1077, 1707, 2810, 2880, 3417, 3570
BveI ACCTGC 1 cut(s) 1108
Cac8I GCNNGC 5 cut(s) 173, 2170, 3299, 3699, 4029
CaiI CAGNNNCTG 1 cut(s) 190
CciI TCATGA 3 cut(s) 801, 855, 3906
CfoI GCGC 4 cut(s) 73, 88, 884, 3697
Cfr10I RCCGGY 1 cut(s) 2135
Cfr9I CCCGGG 1 cut(s) 1173
CseI GACGC 4 cut(s) 27, 535, 558, 4033
CspAI ACCGGT 1 cut(s) 2135
CspCI CAANNNNNGTGG 6 cut(s) 445, 480, 822, 857, 1668, 1703
DraI TTTAAA 1 cut(s) 3756
EaeI YGGCCR 3 cut(s) 595, 1689, 2493
Eam1104I CTCTTC 5 cut(s) 1371, 1442, 1758, 2307, 2316
EarI CTCTTC 5 cut(s) 1371, 1442, 1758, 2307, 2316
EciI GGCGGA 2 cut(s) 29, 680
Ecl136II GAGCTC 2 cut(s) 513, 2791
Eco130I CCWWGG 8 cut(s) 153, 553, 598, 1641, 1926, 2098, 2280, 3522
Eco147I AGGCCT 1 cut(s) 3160
Eco24I GRGCYC 3 cut(s) 515, 1011, 2793
Eco31I GGTCTC 2 cut(s) 1103, 3491
Eco47I GGWCC 9 cut(s) 503, 968, 1169, 1270, 1287, 2133, 2428, 3089, 3098
Eco53kI GAGCTC 2 cut(s) 513, 2791
Eco57I CTGAAG 5 cut(s) 212, 246, 551, 1395, 3228
Eco88I CYCGRG 3 cut(s) 236, 1173, 1622
EcoICRI GAGCTC 2 cut(s) 513, 2791
EcoO109I RGGNCCY 1 cut(s) 1169
EcoRI GAATTC 2 cut(s) 2093, 3924
EcoRII CCWGG 4 cut(s) 1760, 2108, 3160, 3968
EcoT14I CCWWGG 8 cut(s) 153, 553, 598, 1641, 1926, 2098, 2280, 3522
EcoT22I ATGCAT 1 cut(s) 1510
EcoT38I GRGCYC 3 cut(s) 515, 1011, 2793
ErhI CCWWGG 8 cut(s) 153, 553, 598, 1641, 1926, 2098, 2280, 3522
FalI AAGNNNNNCTT 2 cut(s) 1457, 1489
FaqI GGGAC 6 cut(s) 1182, 1203, 1733, 2133, 2146, 2471
FauI CCCGC 3 cut(s) 760, 1229, 1849
FauNDI CATATG 1 cut(s) 878
FbaI TGATCA 1 cut(s) 1554
FokI GGATG 4 cut(s) 2598, 2924, 3196, 3646
FriOI GRGCYC 3 cut(s) 515, 1011, 2793
FspBI CTAG 8 cut(s) 737, 746, 962, 1851, 2010, 3018, 3461, 4028
FspI TGCGCA 1 cut(s) 3696
GlaI GCGC 4 cut(s) 72, 87, 883, 3696
GsaI CCCAGC 2 cut(s) 3505, 3633
GsuI CTGGAG 5 cut(s) 94, 110, 197, 1089, 3991
HaeII RGCGCY 2 cut(s) 74, 89
HaeIII GGCC 7 cut(s) 597, 930, 1152, 1691, 2495, 3160, 3984
HapII CCGG 7 cut(s) 516, 546, 1174, 1576, 2136, 2496, 2825
HgaI GACGC 4 cut(s) 27, 535, 558, 4033
HhaI GCGC 4 cut(s) 73, 88, 884, 3697
Hin1I GRCGYC 4 cut(s) 38, 165, 550, 629
Hin6I GCGC 4 cut(s) 71, 86, 882, 3695
HinP1I GCGC 4 cut(s) 71, 86, 882, 3695
HincII GTYRAC 5 cut(s) 163, 217, 647, 2740, 2844
HindII GTYRAC 5 cut(s) 163, 217, 647, 2740, 2844
HindIII AAGCTT 4 cut(s) 330, 1338, 1471, 1694
HpaI GTTAAC 1 cut(s) 217
HpaII CCGG 7 cut(s) 516, 546, 1174, 1576, 2136, 2496, 2825
HphI GGTGA 6 cut(s) 797, 965, 2785, 2842, 3796, 4016
Hpy99I CGWCG 4 cut(s) 43, 123, 529, 634
Hsp92I GRCGYC 4 cut(s) 38, 165, 550, 629
HspAI GCGC 4 cut(s) 71, 86, 882, 3695
KflI GGGWCCC 1 cut(s) 1169
Ksp22I TGATCA 1 cut(s) 1554
KspAI GTTAAC 1 cut(s) 217
LguI GCTCTTC 1 cut(s) 2307
LmnI GCTCC 8 cut(s) 187, 426, 518, 1108, 1456, 2788, 2878, 3874
LweI GCATC 3 cut(s) 2923, 3174, 3447
MaeI CTAG 8 cut(s) 737, 746, 962, 1851, 2010, 3018, 3461, 4028
MaeIII GTNAC 6 cut(s) 2391, 2648, 3193, 3559, 3645, 3832
MfeI CAATTG 2 cut(s) 1599, 3135
MflI RGATCY 3 cut(s) 1922, 1961, 3527
MhlI GDGCHC 8 cut(s) 243, 358, 515, 1011, 1066, 1630, 2793, 2812
MlsI TGGCCA 2 cut(s) 597, 1691
MluI ACGCGT 1 cut(s) 21
MluNI TGGCCA 2 cut(s) 597, 1691
MlyI GAGTC 7 cut(s) 123, 255, 679, 1802, 2399, 3567, 3982
MmeI TCCRAC 6 cut(s) 142, 697, 852, 2497, 3356, 3455
Mox20I TGGCCA 2 cut(s) 597, 1691
Mph1103I ATGCAT 1 cut(s) 1510
MroXI GAANNNNTTC 4 cut(s) 1341, 2700, 3363, 3893
MscI TGGCCA 2 cut(s) 597, 1691
MslI CAYNNNNRTG 3 cut(s) 2127, 2546, 2954
Msp20I TGGCCA 2 cut(s) 597, 1691
MspA1I CMGCKG 1 cut(s) 3900
MspCI CTTAAG 1 cut(s) 911
MspI CCGG 7 cut(s) 516, 546, 1174, 1576, 2136, 2496, 2825
MspR9I CCNGG 8 cut(s) 1174, 1175, 1762, 2110, 2497, 2826, 3162, 3970
MunI CAATTG 2 cut(s) 1599, 3135
Mva1269I GAATGC 2 cut(s) 1051, 1510
MvaI CCWGG 4 cut(s) 1762, 2110, 3162, 3970
MvnI CGCG 1 cut(s) 23
NciI CCSGG 4 cut(s) 1174, 1175, 2497, 2826
NcoI CCATGG 3 cut(s) 598, 1641, 3522
NdeI CATATG 1 cut(s) 878
NheI GCTAGC 1 cut(s) 4027
NlaIV GGNNCC 9 cut(s) 504, 929, 1170, 1171, 1677, 1924, 2134, 3100, 3529
NmeAIII GCCGAG 2 cut(s) 200, 3327
NmuCI GTSAC 3 cut(s) 2391, 3559, 3832
NsbI TGCGCA 1 cut(s) 3696
NsiI ATGCAT 1 cut(s) 1510
NspI RCATGY 1 cut(s) 3883
PagI TCATGA 3 cut(s) 801, 855, 3906
PceI AGGCCT 1 cut(s) 3160
PciSI GCTCTTC 1 cut(s) 2307
PctI GAATGC 2 cut(s) 1051, 1510
PdmI GAANNNNTTC 4 cut(s) 1341, 2700, 3363, 3893
PfeI GAWTC 8 cut(s) 720, 1070, 2380, 2696, 3035, 3480, 3889, 3910
PflFI GACNNNGTC 2 cut(s) 37, 705
PflMI CCANNNNNTGG 1 cut(s) 2804
PfoI TCCNGGA 1 cut(s) 2108
PinAI ACCGGT 1 cut(s) 2135
PleI GAGTC 7 cut(s) 122, 255, 679, 1801, 2398, 3566, 3981
PpsI GAGTC 7 cut(s) 122, 255, 679, 1801, 2398, 3566, 3981
Ppu21I YACGTR 1 cut(s) 2804
PpuMI RGGWCCY 1 cut(s) 1169
PsiI TTATAA 1 cut(s) 473
Psp124BI GAGCTC 2 cut(s) 515, 2793
Psp1406I AACGTT 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 1169
Psp6I CCWGG 4 cut(s) 1760, 2108, 3160, 3968
PspFI CCCAGC 2 cut(s) 3501, 3629
PspGI CCWGG 4 cut(s) 1760, 2108, 3160, 3968
PspN4I GGNNCC 9 cut(s) 504, 929, 1170, 1171, 1677, 1924, 2134, 3100, 3529
PspPPI RGGWCCY 1 cut(s) 1169
PstI CTGCAG 4 cut(s) 1420, 2947, 3178, 3424
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 3 cut(s) 1922, 1961, 3527
PsyI GACNNNGTC 2 cut(s) 37, 705
RseI CAYNNNNRTG 3 cut(s) 2127, 2546, 2954
SacI GAGCTC 2 cut(s) 515, 2793
SapI GCTCTTC 1 cut(s) 2307
ScaI AGTACT 4 cut(s) 1283, 2375, 3125, 3307
SchI GAGTC 7 cut(s) 123, 255, 679, 1802, 2399, 3567, 3982
ScrFI CCNGG 8 cut(s) 1174, 1175, 1762, 2110, 2497, 2826, 3162, 3970
SduI GDGCHC 8 cut(s) 243, 358, 515, 1011, 1066, 1630, 2793, 2812
SfaNI GCATC 3 cut(s) 2923, 3174, 3447
SfcI CTRYAG 9 cut(s) 948, 1416, 2868, 2898, 2943, 3174, 3420, 3594, 3963
SinI GGWCC 9 cut(s) 503, 968, 1169, 1270, 1287, 2133, 2428, 3089, 3098
SmaI CCCGGG 1 cut(s) 1175
SmiMI CAYNNNNRTG 3 cut(s) 2127, 2546, 2954
SmlI CTYRAG 3 cut(s) 911, 1463, 1768
SmoI CTYRAG 3 cut(s) 911, 1463, 1768
SpeI ACTAGT 1 cut(s) 961
SseBI AGGCCT 1 cut(s) 3160
SspMI CTAG 8 cut(s) 737, 746, 962, 1851, 2010, 3018, 3461, 4028
SstI GAGCTC 2 cut(s) 515, 2793
StuI AGGCCT 1 cut(s) 3160
StyD4I CCNGG 8 cut(s) 1172, 1173, 1760, 2108, 2495, 2824, 3160, 3968
StyI CCWWGG 8 cut(s) 153, 553, 598, 1641, 1926, 2098, 2280, 3522
TaqI TCGA 5 cut(s) 300, 1011, 1933, 2566, 3616
TaqII GACCGA 1 cut(s) 407
TatI WGTACW 7 cut(s) 849, 1281, 2373, 3123, 3305, 3414, 3636
TauI GCSGC 5 cut(s) 86, 622, 665, 943, 3903
TfiI GAWTC 8 cut(s) 720, 1070, 2380, 2696, 3035, 3480, 3889, 3910
TscAI CASTG 7 cut(s) 363, 1084, 1707, 2817, 2880, 3424, 3577
TseFI GTSAC 3 cut(s) 2391, 3559, 3832
Tsp45I GTSAC 3 cut(s) 2391, 3559, 3832
TspGWI ACGGA 4 cut(s) 1685, 2767, 3206, 3828
TspMI CCCGGG 1 cut(s) 1173
TspRI CASTG 7 cut(s) 363, 1084, 1707, 2817, 2880, 3424, 3577
Tth111I GACNNNGTC 2 cut(s) 37, 705
Van91I CCANNNNNTGG 1 cut(s) 2804
Vha464I CTTAAG 1 cut(s) 911
VneI GTGCAC 1 cut(s) 1062
VpaK11BI GGWCC 9 cut(s) 503, 968, 1169, 1270, 1287, 2133, 2428, 3089, 3098
XbaI TCTAGA 2 cut(s) 1850, 2009
XceI RCATGY 1 cut(s) 3883
XcmI CCANNNNNNNNNTGG 1 cut(s) 2190
XmaI CCCGGG 1 cut(s) 1173
XmnI GAANNNNTTC 4 cut(s) 1341, 2700, 3363, 3893
XspI CTAG 8 cut(s) 737, 746, 962, 1851, 2010, 3018, 3461, 4028
ZraI GACGTC 2 cut(s) 166, 630
ZrmI AGTACT 4 cut(s) 1283, 2375, 3125, 3307
Zsp2I ATGCAT 1 cut(s) 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.