MD15G1145300.v1.1
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008072
Physical Location & Seq
Reverse (-)
10756197 .. 10763956
7760 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1145300.v1.1.491

Sequence Viewer

Length: 4260 bp
ATGTGGAACTCGGCGGAGAATGTGTTCGCGAGATCGGCGTCGTTTCGGGAGGTAGGGGAGGATGAGGAGGCGCTGCGGTGGGCCGCGTTGGAGAGGCTCCCGACCTACGCCCGCGCCCGTCGCGGCATTTTCAGGAACGTCGCCGGAGGCACTATGGAGATCGACGTCGGCGAGCTCGAGGCCAAGGAGCAGAAGCTCCTCCTTGACCGTCTCATTAGCTCCGCTGACGACGACCCCGAGCAATTCTTCAATCGCATGCGCCGTAGATTTGACGCAGTAAGATTGAACTTTCCGAAAATCGAGGTCCGATTTCAGAACATGAAGGTTGAAACTTTCGTCCACGTGGGAAGCAGAGCGCTGCCGACTATTCCCAATTTTGTTTTTAATATGACGGAGGCCCTTTTTAGGCAATTGCGAATATACAGGGGGCAGAGACACAAATTAACGATTTTAGACAACGTTAGCGGGATAATAAGACCTTCAAGGTTGACGTTACTATTGGGTGCACCAAGCTCCGGAAAGACTACGCTACTATTGGCTCTTGCCGGACGCCTGGGAGCAGGTTTGCAGACGTCAGGGAACGTCACGTATAATGGACATCGCTTAAAGGAGTTTGTTCCTCAGCGGACATCTGCGTACGTCAGCCAACAGGATTGGCACGCGGCAGAGATGACTGTAAGGGAAACTCTTGAGTTTGCAGGACGATGTCAAGGTGTTGGATTCAAATATGATATGTTAATAGAACTTGCAAGAAGGGAAAAGAGAGACGGGATAAAACCCGATGAAGATCTTGATATATTTATGAAGTCATTAGCTCTTGGGGGGCAGGAGACAAGCCTTACGGTGGAGTACATCATGAAGATTTTAGGGTTGGACATATGTGCTGATACCTTGGTAGGAGATGAAATGCTCAAGGGAATCTCAGGGGGTCAAAAGAAGCGGCTTACTACAGGTGAATTATTAGTAGGTCCGGCAAGAGTTCTGTTCATGGATGATATTTCGACTGGTCTTGATAGTTCAACTACTTATCAAATCATCAAATATCTAAGGCATTCAACTCGGGCACTAGATGCAACCACTGTCATATCTTTGCTTCAACCTGCTCCAGAGACCTACGAGTTGTTTGATGATGTTATACTTTTGTGTGAGGGCCAAATTGTGTATCAGGGACCCCGCGAAACAGCTCTTGATTTTTTTTCATATGTGGGATTTAGATGTCCACAGAGAAAGAATGTGGCAGACTTCTTGCAAGAAGTTATATCAGAGAAGGACCAAGAGCAGTATTGGTCCAATCCTGATCTCCCGTACTGCTATGTTCCACCGGCAAAGTTTGTTGACGCTTATCATTTATTTCAAGCTGGGAAGACTTTGTCTGAAGAACTAGAAGTTCCTTTTGATAAACGCTATAACCATCCTGCAGCTTTAGCAACGTTTCTTTATGGCATGAAGAGGTGTGAGCTTCTCAAAACAAGCTTTAACTGGCAACTTCTACTCATGAAGCGAAATGCATTTATCTATGCTTTCAAGTTCATTCAGCTTCTTTTGGTTGCTATGGTCACAATGAGTGTTTTTTTCCGGTCAACATTACACCACAATACAATTGATGATGGAGGCCTATATCTCGGTGCTCTATACTTTTCCATGGTTATTATTCTTTTCAATGGCTTCATGGAGGTGTCAATGTTGGTGGCCAAGCTTCCGGTACTTTACAAGCATAGGGACTTGCACTTCTACCCTAGCTGGGTCTATACCATTCCGTCCTGGGCCTTAAGTGTTCCAAATTCATTCATAGAGTCTGGCTTGTGGGTGGCAATCACATACTATGTCATTGGATTTGATCCTTCAATTACTAGATTCTGTGGGCAGTTCTTGATATATTTCTTGCTACACCAGATGTCAGTGGCTCTCTTCCGTCTTATTGGATCCTTGGGTCGAAATATGATAGTTGCTAACACTTTTGGATCCTTTGCTATGTTAGTTGTTATGGGTCTTGGAGGATACGTCATCTCAAGAGATCGTGTACCAAAGTGGTGGATATGGGGTTTCTGGGTTTCTCCCTTGATGTATGCTCAAAATGCAGCTTCCGTAAATGAATTCCTTGGACATAAATGGGATAAGAGAGTTGGAAACGAGACCATTCCATTAGGCGAGGCATTGCTAAGAGCACGCAGTTTGTTTCCACAGAGCTACTGGTATTGGATTGGTGCTGGTGCTTTGCTTGGATATACAGTTCTATTCAACGTCCTCTTCACATTCTTCCTAGCTTACCTTAATCCCTTGGGGAAACGACAAGCAGTAGTCACTAAGGACGAGCTTCAAGACAGAGAAAGGAGAAGAAAAGGAGACACTGTTGTTATTGAGCTGAGACAGTACTTGCAGCATTCAGAGTCATTAAATGGAAAATATTTGAAGCAGAGAGGAATGGTGCTCCCATTTCAACAACTTTCCATGTCTTTCAGCAATATCAATTACTATGTCGATGTCCCTCAGGAATTGAAGCAGCAGGGCATTCAAGAAGAGAAATTGCAGTTGTTGAGTAATGTCACTGGCACATTTAGGCCCGGGGTGCTTACAGCATTGGTTGGAGTAAGTGGTGCTGGGAAAACCACCCTCATGGACGTATTAGCTGGTCGAAAAACCGGTGGGATGATTGAAGGGAGCATTCATATATCTGGTTACCCAAAAAGACAAGAAACTTTTGCGAGAATTTCTGGTTACTGCGAGCAGTCCGATATTCATTCCCCCTGCTTGACTGTTCTGGAATCACTTCTCTTCTCTGTTTGGCTTCGATTACCATCAGATGTTGACTTGGAGACACAGAGGGCGTTTGTAGAAGAGGTGATGGAACTAGTGGAGCTCACCCCATTAAGTGGGGCATTGGTTGGTCTACCGGGGGTAGATGGTTTGTCGACAGAACAGCGAAAAAGGCTAACGATAGCTGTTGAGCTGGTCGCCAACCCCTCTATAGTGTTCATGGATGAACCCACATCAGGGTTGGATGCACGGTCTGCAGCTATTGTGATGAGGACTGTGAGGAATATAGTCAATACTGGACGCACTATAGTGTGCACAATCCACCAGCCTAGCATAGACATCTTTGAATCCTTTGATGAACTTCTGTTTTTGAAGCGCGGAGGAGAGCTCATATATGCTGGTCCACTTGGTCCCAGGTCTTGTGAACTCATTAAATACTTTGAGGCAGTCGAGGGAGTAGAAAAGATCAGGCCTGGATATAATCCTGCCACATGGATGCTTGATGTTACTTCTACAGTTGAAGAAAGTCGCCTGGGGGTGGATTTTGCAGAAGTTTACCGTAGATCTAACTTGTTTCAACGTAATAAAGAGTTGGTTGAAAACCTAAGCAAACCAAGCGCCAATTCGAAGGACTTGAACTTTCCAAGCAAGTACTCTCAGTCATTTGTCGAACAATTTCTCACTTGTCTTTGGAAGCAAAATCTGTCTTACTGGCGAAACCCTCAGTACACTGCAGTTCGCTTTTTTTATACAGTCATCATCTCGTTTATGTTGGGAACAATTTGTTGGAAATTCGGAGACAAGAGGGAGACCCAGCAGGATCTGTTAAACGCTATGGGGTCCATGTATGCAGCAATCCTATTTAGCGGAATCACCAATGCCAGTGCTGTTCAACCTGTTATTTCTGTAGAACGATTTGTTTCGTATCGAGAGAGAGCTGCTGGGATGTACTCGGCCTTACCATTTGCATTTGCTCAGGTCGTAATAGAGCTCCCTTATGTGTTTGCACAAGCGATTATCTACTGCGCAATTTTTTACTACACGGCCTCATTTGAGTGGACTCTTTTCAAGTTTCTTTGGTACATATTCTTTATGTACTTCACAATGCTATATTTCACCTTCTACGGAATGATGACAATCGCTGTCACGCCAAATCATAATGTCGCTGCTATAATTGCTGCTCCGATTTACATGCTGTGGAACCTCTTCAGCGGCTTTATGATTCCTCACAAGCGAATTCCAATCTGGTGGAGATGGTATTACTGGGCAGACCCTATCGCCTGGAGTCTGTACGGCCTCTTTGTTTCACAGTACGGCGAGGATGACAGTCTTGTGAAGCTAGCTGACGGAATCCATAAGATGCCTGTAAGGCAGTTGCTCGAAGTAGGGTTTGGTTACAAGCATGATTTTCTGGGTGTAGCCGGCATTGCGGTGGTCGGTTTCTGCGTGTTTTTCGCCTTTGTTTTTGCCTTTGCAATAAAAGCATTCAACTTCCAGAGGAGATGA

Protein Analysis

1420

Amino Acids

161.75

Weight (kDa)

8.33

Isoelectric Point (pI)

40.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 74 - 126 1.6e-06 ABC-transporter N-terminal
ABC_tran PF00005 151 - 332 2.5e-15 ABC transporter
ABC2_membrane PF01061 488 - 699 3.3e-45 ABC-2 type transporter
PDR_assoc PF08370 704 - 767 9.2e-28 Plant PDR ABC transporter associated
ABC_tran PF00005 845 - 997 6.4e-19 ABC transporter
ABC2_membrane PF01061 1142 - 1356 5.1e-56 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 168, 575
Acc16I TGCGCA 1 cut(s) 3781
Acc36I ACCTGC 2 cut(s) 551, 1108
AccB7I CCANNNNNTGG 1 cut(s) 2870
AccI GTMKAC 2 cut(s) 2887, 2909
AccII CGCG 7 cut(s) 29, 86, 114, 123, 662, 1176, 3132
AccIII TCCGGA 1 cut(s) 515
AclI AACGTT 2 cut(s) 459, 1430
AclWI GGATC 6 cut(s) 1832, 1917, 1930, 1956, 1969, 3582
AcoI YGGCCR 1 cut(s) 1689
AcsI RAATTY 5 cut(s) 1780, 2093, 2706, 3545, 3990
AcuI CTGAAG 2 cut(s) 1395, 3946
AcvI CACGTG 1 cut(s) 343
AcyI GRCGYC 4 cut(s) 38, 165, 550, 572
AfeI AGCGCT 1 cut(s) 357
AfiI CCNNNNNNNGG 8 cut(s) 405, 515, 844, 1741, 2870, 2990, 2991, 3292
AflII CTTAAG 1 cut(s) 1768
AgeI ACCGGT 1 cut(s) 2639
AhlI ACTAGT 1 cut(s) 2848
AjnI CCWGG 6 cut(s) 552, 1760, 3167, 3226, 3285, 4034
AjuI GAANNNNNNNTTGG 6 cut(s) 3361, 3393, 3523, 3555, 4128, 4160
AleI CACNNNNGTG 1 cut(s) 3062
AloI GAACNNNNNNTCC 4 cut(s) 8, 40, 2214, 2246
Alw21I GWGCWC 9 cut(s) 177, 508, 1630, 2167, 2430, 2859, 3071, 3144, 3747
Alw44I GTGCAC 2 cut(s) 504, 3067
AlwI GGATC 6 cut(s) 1832, 1917, 1930, 1956, 1969, 3582
AlwNI CAGNNNCTG 1 cut(s) 3577
Ama87I CYCGRG 4 cut(s) 176, 236, 1059, 2561
Aor13HI TCCGGA 1 cut(s) 515
Aor51HI AGCGCT 1 cut(s) 357
ApaLI GTGCAC 2 cut(s) 504, 3067
ApoI RAATTY 5 cut(s) 1780, 2093, 2706, 3545, 3990
AsiGI ACCGGT 1 cut(s) 2639
Asp700I GAANNNNTTC 4 cut(s) 23, 2766, 3429, 3959
AspLEI GCGC 7 cut(s) 73, 116, 261, 358, 3132, 3374, 3782
AsuC2I CCSGG 3 cut(s) 2562, 2563, 2892
AsuHPI GGTGA 5 cut(s) 965, 2851, 2851, 3619, 3862
AsuII TTCGAA 1 cut(s) 3380
AsuNHI GCTAGC 1 cut(s) 4093
AvaI CYCGRG 4 cut(s) 176, 236, 1059, 2561
AvaII GGWCC 8 cut(s) 304, 968, 1169, 1270, 1287, 3155, 3164, 3594
AxyI CCTNAGG 1 cut(s) 2487
BaeGI GKGCMC 3 cut(s) 508, 1066, 3071
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BalI TGGCCA 1 cut(s) 1691
BamHI GGATCC 2 cut(s) 1922, 1961
BanII GRGCYC 4 cut(s) 177, 2859, 3144, 3747
BbrPI CACGTG 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 1370
Bbv12I GWGCWC 9 cut(s) 177, 508, 1630, 2167, 2430, 2859, 3071, 3144, 3747
BbvCI CCTCAGC 1 cut(s) 621
BccI CCATC 6 cut(s) 1419, 1601, 2803, 2836, 2894, 4002
BceAI ACGGC 4 cut(s) 246, 3813, 4063, 4084
BcgI CGANNNNNNTGC 4 cut(s) 1040, 1074, 3928, 3962
BciT130I CCWGG 6 cut(s) 554, 1762, 3169, 3228, 3287, 4036
BciVI GTATCC 1 cut(s) 1991
BcnI CCSGG 3 cut(s) 2562, 2563, 2892
BcuI ACTAGT 1 cut(s) 2848
BfaI CTAG 8 cut(s) 1067, 1382, 1737, 1851, 2261, 2849, 3084, 4094
BfmI CTRYAG 8 cut(s) 948, 1416, 2964, 3009, 3060, 3267, 3486, 3660
BfoI RGCGCY 3 cut(s) 74, 359, 3375
BfrI CTTAAG 1 cut(s) 1768
BfuAI ACCTGC 2 cut(s) 551, 1108
BfuI GTATCC 1 cut(s) 1991
BglI GCCNNNNNGGC 1 cut(s) 4123
BglII AGATCT 2 cut(s) 787, 3317
BmcAI AGTACT 2 cut(s) 2372, 3407
Bme1390I CCNGG 9 cut(s) 554, 1762, 2562, 2563, 2892, 3169, 3228, 3287, 4036
Bme18I GGWCC 8 cut(s) 304, 968, 1169, 1270, 1287, 3155, 3164, 3594
BmeT110I CYCGRG 4 cut(s) 176, 236, 1059, 2561
BmiI GGNNCC 8 cut(s) 98, 1170, 1171, 1924, 1963, 3166, 3595, 3956
BmrFI CCNGG 9 cut(s) 554, 1762, 2562, 2563, 2892, 3169, 3228, 3287, 4036
BmrI ACTGGG 1 cut(s) 4027
BmsI GCATC 4 cut(s) 1060, 2989, 3240, 4104
BmtI GCTAGC 1 cut(s) 4097
BmuI ACTGGG 1 cut(s) 4027
BpiI GAAGAC 1 cut(s) 1370
BplI GAGNNNNNCTC 2 cut(s) 3126, 3158
BpmI CTGGAG 2 cut(s) 1089, 4057
Bpu10I CCTNAGC 3 cut(s) 621, 3359, 3729
Bpu14I TTCGAA 1 cut(s) 3380
BpuEI CTTGAG 3 cut(s) 710, 896, 1993
BpuMI CCSGG 3 cut(s) 2562, 2563, 2892
BsaAI YACGTR 2 cut(s) 343, 588
BsaBI GATNNNNATC 3 cut(s) 786, 2794, 3890
BsaHI GRCGYC 4 cut(s) 38, 165, 550, 572
BsaI GGTCTC 3 cut(s) 1103, 2126, 3557
BsaWI WCCGGW 4 cut(s) 515, 1575, 1699, 2639
BsaXI ACNNNNNCTCC 6 cut(s) 8, 38, 1986, 2016, 2334, 2364
Bsc4I CCNNNNNNNGG 8 cut(s) 405, 515, 844, 1741, 2870, 2990, 2991, 3292
Bse118I RCCGGY 3 cut(s) 1321, 2639, 4175
Bse1I ACTGG 8 cut(s) 1009, 1484, 2195, 2551, 3055, 3470, 3636, 4022
Bse21I CCTNAGG 1 cut(s) 2487
Bse3DI GCAATG 2 cut(s) 2153, 4179
Bse8I GATNNNNATC 3 cut(s) 786, 2794, 3890
BseAI TCCGGA 1 cut(s) 515
BseBI CCWGG 6 cut(s) 554, 1762, 3169, 3228, 3287, 4036
BseGI GGATG 9 cut(s) 67, 997, 1411, 2652, 2983, 3004, 3255, 3705, 4081
BseJI GATNNNNATC 3 cut(s) 786, 2794, 3890
BseLI CCNNNNNNNGG 8 cut(s) 405, 515, 844, 1741, 2870, 2990, 2991, 3292
BseMI GCAATG 2 cut(s) 2153, 4179
BseMII CTCAG 7 cut(s) 635, 936, 2354, 2501, 3425, 3491, 3743
BseNI ACTGG 8 cut(s) 1009, 1484, 2195, 2551, 3055, 3470, 3636, 4022
BseRI GAGGAG 3 cut(s) 80, 188, 3150
BseSI GKGCMC 3 cut(s) 508, 1066, 3071
BseYI CCCAGC 5 cut(s) 1358, 1740, 2597, 3567, 3695
Bsh1236I CGCG 7 cut(s) 29, 86, 114, 123, 662, 1176, 3132
BshTI ACCGGT 1 cut(s) 2639
BsiHKAI GWGCWC 9 cut(s) 177, 508, 1630, 2167, 2430, 2859, 3071, 3144, 3747
BsiHKCI CYCGRG 4 cut(s) 176, 236, 1059, 2561
BsiWI CGTACG 1 cut(s) 636
BslFI GGGAC 4 cut(s) 1182, 1733, 2468, 3150
BslI CCNNNNNNNGG 8 cut(s) 405, 515, 844, 1741, 2870, 2990, 2991, 3292
BsmBI CGTCTC 2 cut(s) 215, 759
BsmFI GGGAC 4 cut(s) 1182, 1733, 2468, 3150
BsmI GAATGC 5 cut(s) 1051, 2380, 2508, 2661, 4238
Bso31I GGTCTC 3 cut(s) 1103, 2126, 3557
BsoBI CYCGRG 4 cut(s) 176, 236, 1059, 2561
Bsp119I TTCGAA 1 cut(s) 3380
Bsp13I TCCGGA 1 cut(s) 515
Bsp19I CCATGG 1 cut(s) 1641
Bsp68I TCGCGA 1 cut(s) 29
BspCNI CTCAG 7 cut(s) 634, 935, 2355, 2500, 3424, 3490, 3742
BspEI TCCGGA 1 cut(s) 515
BspFNI CGCG 7 cut(s) 29, 86, 114, 123, 662, 1176, 3132
BspHI TCATGA 2 cut(s) 855, 1494
BspLI GGNNCC 8 cut(s) 98, 1170, 1171, 1924, 1963, 3166, 3595, 3956
BspMAI CTGCAG 3 cut(s) 1420, 3013, 3490
BspMI ACCTGC 2 cut(s) 551, 1108
BspOI GCTAGC 1 cut(s) 4097
BspPI GGATC 6 cut(s) 1832, 1917, 1930, 1956, 1969, 3582
BspT104I TTCGAA 1 cut(s) 3380
BspTI CTTAAG 1 cut(s) 1768
BspTNI GGTCTC 3 cut(s) 1103, 2126, 3557
BsrDI GCAATG 2 cut(s) 2153, 4179
BsrFI RCCGGY 3 cut(s) 1321, 2639, 4175
BsrI ACTGG 8 cut(s) 1009, 1484, 2195, 2551, 3055, 3470, 3636, 4022
BssAI RCCGGY 3 cut(s) 1321, 2639, 4175
BssNI GRCGYC 4 cut(s) 38, 165, 550, 572
BssT1I CCWWGG 6 cut(s) 183, 891, 1641, 1926, 2098, 2277
Bst2UI CCWGG 6 cut(s) 554, 1762, 3169, 3228, 3287, 4036
Bst6I CTCTTC 7 cut(s) 1442, 1913, 2252, 2511, 2777, 2829, 3965
BstACI GRCGYC 4 cut(s) 38, 165, 550, 572
BstAFI CTTAAG 1 cut(s) 1768
BstAPI GCANNNNNTGC 2 cut(s) 1070, 3008
BstBAI YACGTR 2 cut(s) 343, 588
BstBI TTCGAA 1 cut(s) 3380
BstC8I GCNNGC 8 cut(s) 112, 173, 257, 660, 2167, 2723, 4095, 4177
BstDSI CCRYGG 1 cut(s) 1641
BstEII GGTNACC 1 cut(s) 2675
BstF5I GGATG 9 cut(s) 67, 997, 1411, 2652, 2983, 3004, 3255, 3705, 4081
BstFNI CGCG 7 cut(s) 29, 86, 114, 123, 662, 1176, 3132
BstH2I RGCGCY 3 cut(s) 74, 359, 3375
BstHHI GCGC 7 cut(s) 73, 116, 261, 358, 3132, 3374, 3782
BstNI CCWGG 6 cut(s) 554, 1762, 3169, 3228, 3287, 4036
BstNSI RCATGY 2 cut(s) 259, 3949
BstPI GGTNACC 1 cut(s) 2675
BstSCI CCNGG 9 cut(s) 552, 1760, 2560, 2561, 2890, 3167, 3226, 3285, 4034
BstSFI CTRYAG 8 cut(s) 948, 1416, 2964, 3009, 3060, 3267, 3486, 3660
BstSLI GKGCMC 3 cut(s) 508, 1066, 3071
BstUI CGCG 7 cut(s) 29, 86, 114, 123, 662, 1176, 3132
BstV2I GAAGAC 1 cut(s) 1370
BstX2I RGATCY 5 cut(s) 787, 1922, 1961, 3317, 3574
BstXI CCANNNNNNTGG 3 cut(s) 2031, 2614, 4002
BstYI RGATCY 5 cut(s) 787, 1922, 1961, 3317, 3574
Bsu36I CCTNAGG 1 cut(s) 2487
BsuI GTATCC 1 cut(s) 1991
BtgI CCRYGG 1 cut(s) 1641
BtgZI GCGATG 1 cut(s) 584
BtsCI GGATG 9 cut(s) 67, 997, 1411, 2652, 2983, 3004, 3255, 3705, 4081
BtsI GCAGTG 1 cut(s) 3483
BtsIMutI CAGTG 6 cut(s) 1077, 1905, 2346, 2544, 3483, 3643
BtuMI TCGCGA 1 cut(s) 29
BveI ACCTGC 2 cut(s) 551, 1108
Cac8I GCNNGC 8 cut(s) 112, 173, 257, 660, 2167, 2723, 4095, 4177
CaiI CAGNNNCTG 1 cut(s) 3577
CciI TCATGA 2 cut(s) 855, 1494
CfoI GCGC 7 cut(s) 73, 116, 261, 358, 3132, 3374, 3782
Cfr10I RCCGGY 3 cut(s) 1321, 2639, 4175
Cfr9I CCCGGG 1 cut(s) 2561
CseI GACGC 5 cut(s) 27, 281, 558, 1346, 3063
CspAI ACCGGT 1 cut(s) 2639
CspCI CAANNNNNGTGG 2 cut(s) 1668, 1703
EaeI YGGCCR 1 cut(s) 1689
Eam1104I CTCTTC 7 cut(s) 1442, 1913, 2252, 2511, 2777, 2829, 3965
EarI CTCTTC 7 cut(s) 1442, 1913, 2252, 2511, 2777, 2829, 3965
EciI GGCGGA 1 cut(s) 29
Ecl136II GAGCTC 4 cut(s) 175, 2857, 3142, 3745
Eco130I CCWWGG 6 cut(s) 183, 891, 1641, 1926, 2098, 2277
Eco147I AGGCCT 2 cut(s) 1614, 3226
Eco24I GRGCYC 4 cut(s) 177, 2859, 3144, 3747
Eco31I GGTCTC 3 cut(s) 1103, 2126, 3557
Eco47I GGWCC 8 cut(s) 304, 968, 1169, 1270, 1287, 3155, 3164, 3594
Eco47III AGCGCT 1 cut(s) 357
Eco53kI GAGCTC 4 cut(s) 175, 2857, 3142, 3745
Eco57I CTGAAG 2 cut(s) 1395, 3946
Eco72I CACGTG 1 cut(s) 343
Eco81I CCTNAGG 1 cut(s) 2487
Eco88I CYCGRG 4 cut(s) 176, 236, 1059, 2561
Eco91I GGTNACC 1 cut(s) 2675
EcoICRI GAGCTC 4 cut(s) 175, 2857, 3142, 3745
EcoO109I RGGNCCY 2 cut(s) 397, 1169
EcoO65I GGTNACC 1 cut(s) 2675
EcoRI GAATTC 2 cut(s) 2093, 3990
EcoRII CCWGG 6 cut(s) 552, 1760, 3167, 3226, 3285, 4034
EcoT14I CCWWGG 6 cut(s) 183, 891, 1641, 1926, 2098, 2277
EcoT22I ATGCAT 1 cut(s) 1510
EcoT38I GRGCYC 4 cut(s) 177, 2859, 3144, 3747
ErhI CCWWGG 6 cut(s) 183, 891, 1641, 1926, 2098, 2277
Esp3I CGTCTC 2 cut(s) 215, 759
FaqI GGGAC 4 cut(s) 1182, 1733, 2468, 3150
FauI CCCGC 3 cut(s) 119, 458, 1181
FauNDI CATATG 2 cut(s) 878, 1201
FblI GTMKAC 2 cut(s) 2887, 2909
FokI GGATG 9 cut(s) 74, 1004, 1398, 2659, 2990, 3011, 3262, 3712, 4088
FriOI GRGCYC 4 cut(s) 177, 2859, 3144, 3747
FspBI CTAG 8 cut(s) 1067, 1382, 1737, 1851, 2261, 2849, 3084, 4094
FspI TGCGCA 1 cut(s) 3781
GlaI GCGC 7 cut(s) 72, 115, 260, 357, 3131, 3373, 3781
GsaI CCCAGC 5 cut(s) 1362, 1744, 2601, 3571, 3699
GsuI CTGGAG 2 cut(s) 1089, 4057
HaeII RGCGCY 3 cut(s) 74, 359, 3375
HgaI GACGC 5 cut(s) 27, 281, 558, 1346, 3063
HhaI GCGC 7 cut(s) 73, 116, 261, 358, 3132, 3374, 3782
Hin1I GRCGYC 4 cut(s) 38, 165, 550, 572
Hin6I GCGC 7 cut(s) 71, 114, 259, 356, 3130, 3372, 3780
HinP1I GCGC 7 cut(s) 71, 114, 259, 356, 3130, 3372, 3780
HincII GTYRAC 5 cut(s) 489, 1336, 1581, 2806, 2910
HindII GTYRAC 5 cut(s) 489, 1336, 1581, 2806, 2910
HindIII AAGCTT 2 cut(s) 1471, 1694
HphI GGTGA 5 cut(s) 965, 2851, 2851, 3619, 3862
Hpy99I CGWCG 6 cut(s) 43, 123, 143, 167, 170, 233
HpyAV CCTTC 8 cut(s) 316, 489, 747, 1261, 1851, 2648, 3376, 3883
Hsp92I GRCGYC 4 cut(s) 38, 165, 550, 572
HspAI GCGC 7 cut(s) 71, 114, 259, 356, 3130, 3372, 3780
KflI GGGWCCC 1 cut(s) 1169
Kpn2I TCCGGA 1 cut(s) 515
KroI GCCGGC 1 cut(s) 4175
KroNI GCCGGC 1 cut(s) 4177
LweI GCATC 4 cut(s) 1060, 2989, 3240, 4104
MaeI CTAG 8 cut(s) 1067, 1382, 1737, 1851, 2261, 2849, 3084, 4094
MfeI CAATTG 2 cut(s) 410, 1599
MflI RGATCY 5 cut(s) 787, 1922, 1961, 3317, 3574
MlsI TGGCCA 1 cut(s) 1691
MluNI TGGCCA 1 cut(s) 1691
MlyI GAGTC 4 cut(s) 1802, 2396, 3808, 4048
MmeI TCCRAC 7 cut(s) 69, 697, 852, 2104, 2563, 2976, 3521
Mox20I TGGCCA 1 cut(s) 1691
Mph1103I ATGCAT 1 cut(s) 1510
MroI TCCGGA 1 cut(s) 515
MroNI GCCGGC 1 cut(s) 4175
MroXI GAANNNNTTC 4 cut(s) 23, 2766, 3429, 3959
MscI TGGCCA 1 cut(s) 1691
MslI CAYNNNNRTG 6 cut(s) 1673, 2612, 3062, 3248, 3808, 4184
Msp20I TGGCCA 1 cut(s) 1691
MspA1I CMGCKG 3 cut(s) 224, 625, 3966
MspCI CTTAAG 1 cut(s) 1768
MspR9I CCNGG 9 cut(s) 554, 1762, 2562, 2563, 2892, 3169, 3228, 3287, 4036
MunI CAATTG 2 cut(s) 410, 1599
Mva1269I GAATGC 5 cut(s) 1051, 2380, 2508, 2661, 4238
MvaI CCWGG 6 cut(s) 554, 1762, 3169, 3228, 3287, 4036
MvnI CGCG 7 cut(s) 29, 86, 114, 123, 662, 1176, 3132
NaeI GCCGGC 1 cut(s) 4177
NciI CCSGG 3 cut(s) 2562, 2563, 2892
NcoI CCATGG 1 cut(s) 1641
NdeI CATATG 2 cut(s) 878, 1201
NgoMIV GCCGGC 1 cut(s) 4175
NheI GCTAGC 1 cut(s) 4093
NlaIV GGNNCC 8 cut(s) 98, 1170, 1171, 1924, 1963, 3166, 3595, 3956
NmeAIII GCCGAG 1 cut(s) 3686
NmuCI GTSAC 5 cut(s) 583, 1555, 2299, 2542, 3898
NruI TCGCGA 1 cut(s) 29
NsbI TGCGCA 1 cut(s) 3781
NsiI ATGCAT 1 cut(s) 1510
NspI RCATGY 2 cut(s) 259, 3949
NspV TTCGAA 1 cut(s) 3380
OliI CACNNNNGTG 1 cut(s) 3062
PaeI GCATGC 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 176
PagI TCATGA 2 cut(s) 855, 1494
PceI AGGCCT 2 cut(s) 1614, 3226
PcsI WCGNNNNNNNCGW 5 cut(s) 168, 174, 234, 259, 4197
PctI GAATGC 5 cut(s) 1051, 2380, 2508, 2661, 4238
PdiI GCCGGC 1 cut(s) 4177
PdmI GAANNNNTTC 4 cut(s) 23, 2766, 3429, 3959
PfeI GAWTC 8 cut(s) 720, 918, 1854, 2762, 3101, 3624, 3976, 4104
Pfl23II CGTACG 1 cut(s) 636
PflFI GACNNNGTC 2 cut(s) 705, 1369
PflMI CCANNNNNTGG 1 cut(s) 2870
PinAI ACCGGT 1 cut(s) 2639
PleI GAGTC 4 cut(s) 1801, 2395, 3808, 4047
PmaCI CACGTG 1 cut(s) 343
PmlI CACGTG 1 cut(s) 343
PpsI GAGTC 4 cut(s) 1801, 2395, 3808, 4047
Ppu21I YACGTR 2 cut(s) 343, 588
PpuMI RGGWCCY 1 cut(s) 1169
Psp124BI GAGCTC 4 cut(s) 177, 2859, 3144, 3747
Psp1406I AACGTT 2 cut(s) 459, 1430
Psp5II RGGWCCY 1 cut(s) 1169
Psp6I CCWGG 6 cut(s) 552, 1760, 3167, 3226, 3285, 4034
PspCI CACGTG 1 cut(s) 343
PspEI GGTNACC 1 cut(s) 2675
PspFI CCCAGC 5 cut(s) 1358, 1740, 2597, 3567, 3695
PspGI CCWGG 6 cut(s) 552, 1760, 3167, 3226, 3285, 4034
PspLI CGTACG 1 cut(s) 636
PspN4I GGNNCC 8 cut(s) 98, 1170, 1171, 1924, 1963, 3166, 3595, 3956
PspPPI RGGWCCY 1 cut(s) 1169
PspXI VCTCGAGB 1 cut(s) 176
PstI CTGCAG 3 cut(s) 1420, 3013, 3490
PstNI CAGNNNCTG 1 cut(s) 3577
PsuI RGATCY 5 cut(s) 787, 1922, 1961, 3317, 3574
PsyI GACNNNGTC 2 cut(s) 705, 1369
RruI TCGCGA 1 cut(s) 29
RseI CAYNNNNRTG 6 cut(s) 1673, 2612, 3062, 3248, 3808, 4184
SacI GAGCTC 4 cut(s) 177, 2859, 3144, 3747
SalI GTCGAC 1 cut(s) 2908
ScaI AGTACT 2 cut(s) 2372, 3407
SchI GAGTC 4 cut(s) 1802, 2396, 3808, 4048
ScrFI CCNGG 9 cut(s) 554, 1762, 2562, 2563, 2892, 3169, 3228, 3287, 4036
SfaNI GCATC 4 cut(s) 1060, 2989, 3240, 4104
SfcI CTRYAG 8 cut(s) 948, 1416, 2964, 3009, 3060, 3267, 3486, 3660
Sfr274I CTCGAG 1 cut(s) 176
SfuI TTCGAA 1 cut(s) 3380
SinI GGWCC 8 cut(s) 304, 968, 1169, 1270, 1287, 3155, 3164, 3594
SlaI CTCGAG 1 cut(s) 176
SmaI CCCGGG 1 cut(s) 2563
SmiMI CAYNNNNRTG 6 cut(s) 1673, 2612, 3062, 3248, 3808, 4184
SmlI CTYRAG 5 cut(s) 176, 689, 911, 1768, 2008
SmoI CTYRAG 5 cut(s) 176, 689, 911, 1768, 2008
SpeI ACTAGT 1 cut(s) 2848
SphI GCATGC 1 cut(s) 259
SseBI AGGCCT 2 cut(s) 1614, 3226
SspI AATATT 1 cut(s) 2405
SspMI CTAG 8 cut(s) 1067, 1382, 1737, 1851, 2261, 2849, 3084, 4094
SstI GAGCTC 4 cut(s) 177, 2859, 3144, 3747
StuI AGGCCT 2 cut(s) 1614, 3226
StyD4I CCNGG 9 cut(s) 552, 1760, 2560, 2561, 2890, 3167, 3226, 3285, 4034
StyI CCWWGG 6 cut(s) 183, 891, 1641, 1926, 2098, 2277
TatI WGTACW 6 cut(s) 849, 2370, 3405, 3480, 3702, 3849
TauI GCSGC 5 cut(s) 86, 126, 665, 943, 3969
TfiI GAWTC 8 cut(s) 720, 918, 1854, 2762, 3101, 3624, 3976, 4104
TscAI CASTG 6 cut(s) 1084, 1905, 2353, 2551, 3490, 3643
TseFI GTSAC 5 cut(s) 583, 1555, 2299, 2542, 3898
Tsp45I GTSAC 5 cut(s) 583, 1555, 2299, 2542, 3898
TspGWI ACGGA 6 cut(s) 407, 1746, 1901, 2074, 3894, 4116
TspMI CCCGGG 1 cut(s) 2561
TspRI CASTG 6 cut(s) 1084, 1905, 2353, 2551, 3490, 3643
Tth111I GACNNNGTC 2 cut(s) 705, 1369
Van91I CCANNNNNTGG 1 cut(s) 2870
Vha464I CTTAAG 1 cut(s) 1768
VneI GTGCAC 2 cut(s) 504, 3067
VpaK11BI GGWCC 8 cut(s) 304, 968, 1169, 1270, 1287, 3155, 3164, 3594
XapI RAATTY 5 cut(s) 1780, 2093, 2706, 3545, 3990
XceI RCATGY 2 cut(s) 259, 3949
XcmI CCANNNNNNNNNTGG 2 cut(s) 2187, 2992
XhoI CTCGAG 1 cut(s) 176
XmaI CCCGGG 1 cut(s) 2561
XmiI GTMKAC 2 cut(s) 2887, 2909
XmnI GAANNNNTTC 4 cut(s) 23, 2766, 3429, 3959
XspI CTAG 8 cut(s) 1067, 1382, 1737, 1851, 2261, 2849, 3084, 4094
ZraI GACGTC 2 cut(s) 166, 573
ZrmI AGTACT 2 cut(s) 2372, 3407
Zsp2I ATGCAT 1 cut(s) 1510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.