Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008072

Physical Location & Seq

Genomic Coordinates Reverse (-)

3276656 .. 3284623

Gene Structure 7968 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1040300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4260 bp

ATGTGGAACTCGGCGGAGAACGTGTTCGCGAGATCGGGGTCGTTTCGGGAGGAAGGGGACGATGAGGAAGCGCTGCGGTGGGCCGCGCTGGAGCGGCTCCCGACCTACGCTCGAGTACGGCGCGGCATTTTCAGAAACGTCGTCGGAGACACCATGGAGATCGACGTCAGCGAGCTCGAGGCTAAGGAGCAGAAGCTCCTTCTCGACCGGCTTGTTAGCTCTGCCGACGACGACCCCGAGCAATTTTTCGATCGCATGCGCCGAAGATTTGACGCAGTAGCATTGAATTTTCCGAAGATCGAGGTCCGATTTCAGAAGTTGAAGGTTGAAGCTTTCGTCCACGTCGGAAGCAGAGCGCTGCCGACTATTCCCAATTTTGTTTTTAATATGGCGGAGGCCCTTTTTAGGCAATTGCGAATATACAGGGGGCAGAGAAGCAAGTTAACGATTTTAGACAACATTAGCGGGATTGTAAGACCTTCAAGATTGACATTACTATTGGGTCCACCAAGCTCCGGAAAGACCACGCTACTTTTGGCTCTTGCCGGGCGCCTGGGAACCGGTTTGCAGATGTCAGGGAACGTCACGTATAACGGACATGGCTTAAGCGAGTTTGTCCCTCAGCGGACATCTGCTTATGTCAGCCAACAGGATTGGCACGTAGCAGAGATGACTGTAAGGGAAACTCTTGAGTTTGCCGGACGATGTCAAGGTGTTGGAACCAAATATGATATGTTAGTAGAACTTGCAAGAAGAGAGAAGTGCGCTGGGATAATACCCGATGAAGATCTTGATATATTCATGAAGTCATTAGCTCTTGGGGGGAAGGAGACAAGCCTTGTGGTGGAGTACATCATGAAGATTTTAGGGTTGGACATATGTGCTGATACATTGGTTGGAGATGAAATGCTCAAGGGAATCTCAGGGGGGCAAAAGAAGCGGCTTACTACAGGGGAATTATTAGTAGGTCCAGCGAGAGTTCTGTTCATGGATGAAATATCAACTGGTCTTGACAGTTCTACTACTTATCAAATCATCAAATATCTAAGGCATTCAACTCGAGCACTTGATGCAACCACTGTCATATCTTTGCTTCAACCTGCTCCAGAGACCTATGAATTGTTTGATGATGTTATACTTTTGTGTGAGGGCCAGATTGTGTATCAGGGACCCCGCGAAACTGCTCTTGATTTCTTTTCATATATGGGATTTAGATGTCCACTGAGAAAGAATGTGGCAGACTTCTTGCAAGAAGTTATATCAAAGAAGGACCAAGAGCAGTACTGGTCCAATCCTGATCTCCCGTACCGCTATGTTCCACCGGCAAAGTTTGTTGATGCTTATCGTTTATTTCAAGCTGGGAAGACTTTGTCTGAAGAACTAGATGTTCCTTTTGATAAACGCTATAACCATCCTGCAGCTTTAGCTACGTCTCTCTATGGCGTGAAGAGGTGTGAGCTTCTCAAAACAAGCTATAACTGGCAACTTCTTCTAATGAAGCGAAATGCATTTATCTACATTTTCAAGTTCATTCAGCTTCTTTTTGTTGCTATGGTCACAATGAGTGTTTTTTTCCGGTCAACATTGCACCACAATACAATTGATGATGGAGGCCTATATCTCGGCGCACTATACTTTTCCATGGTTATTATTCTTTTCAATGGCTTCATGGAGGTGTCAATGTTGGTTGCCAAGCTTCCGGTACTTTACAAGCATAGGGACTTGCATTTCTACCCTAGCTGGGTCTATACCATTCCGTCCTGGGCCTTAAGTGTTCCAAATTCATTCATAGAGTCTGGTTTCTGGGTGGCAATCACATACTATGTCATTGGATTTGATCCTTCAATTACTAGATTCTGTGGGCAGTTCTTGATATATTTCTTGCTACACCAGATGTCAATAGCTCTCTTCCGTCTTATGGGATCCTTGGGTCGAAATATGATAGTAGCCAACACTTTTGGATCTTTTGCTATGTTAGTTGTTATGGCTCTTGGAGGATACATCATCTCAAAAGATCGTATACCAAAGTGGTGGATATGGGGTTTCTGGTTTTCTCCCTTGATGTATGCTCAAAATGCAGCTTCCGTAAATGAATTCCTTGGACATAAATGGGATAAGAGAATTGGAAACGAGACCATTCCATTAGGCGAGGCATTGCTAAGAGCACGCAGTTTGTTTCCACAGAGCTACTGGTTTTGGATTGGTGCTGGTGCTTTGCTTGGATATACAATTTTATTCAACATGCTCTTCACATTCTTCCTAGCGTACCTTAACCCCTTGGGGAAACGACAAGCAGTAGTCACCAAGGAAGAGCTTCAAGAGAGAGAAAGGAGAAGAAAAGGAGAAACTGTTGTTATTGAGCTGAGACAGTACTTGCAGCATTCAGAGTCATTAAATGCAAAATATTTTAAGCAGAGAGGAATGGTGCTCCCATTTCAACAACTTTCCATGTCTTTCAGCAATATCAATTACTACGTCGATGTCCCTCTGGAATTGAAGCAGCAGGGCATTCAAGAAGAGAAATTGCAGCTGTTGAGTAATGTCACTGGAGCATTTAGGCCTGGGGTGCTTACAGCATTGGTTGGAGTAAGTGGTGCAGGGAAAACCACCCTCATGGATGTATTAGCTGGTCGAAAAACTGGTGGGACGATAGAAGGGAGCATTCATATATCTGGTTATCCAAAAAGACAAGAAACTTTTGCGAGAATTTCTGGTTACTGTGAGCAGTCTGATATTCATTCCCCCTGCTTGACTGTCCTGGAATCGCTTCTCTTCTCTGTTTGGCTTCGGTTACCATCAGATGTTGAGTTGGAGATACAAAGGGCTTTTGTAGAAGAGGTGATGGAACTAGTGGAGCTCACTCCTTTAAGTGGGGCGTTGGTTGGTCTACCGGGGGTGGATGGTTTGTCTACAGAACAAAGAAAAAGGCTAACGATAGCTGTTGAGCTGGTTGCCAACCCCTCAATAGTTTTCATGGATGAACCAACATCAGGGTTGGATGCACGTTCTGCAGCTATTGTGATGAGGACTGTGAGGAATATCGTCAATACTGGACGTACTATTGTATGCACAATCCACCAGCCTAGCATAGACATCTTTGAATCCTTTGATGAGCTCCTATTTTTGAAGCGTGGAGGAGAGCTCATATATGCTGGTCCACTTGGTCCCAAGTCTTGTGAACTCATCAAGTACTTTGAGGCCGTCGAAGGAGTAGAAAAGATCAGGCCTGGATATAATCCTGCCACATGGATGCTTGATGTTACTTCTACAGTTGAAGAAAGTCGTCTAGGTGTGGATTTTGCAGAAGTTTACCGAAGTTCTAACTTGTTTCGACATAACAAAGAGTTGGTTGAAATCCTAAGCAAGCCAAGCGCCAATTCGAAAGAATTAAACTTTCCAACCAAGTACTCTCAATCATTTGTCGAACAATTTCTCACTTGTCTTTGGAAGCAAAATCTGTCTTACTGGCGGAACCCTCAGTACACTGCAGTTCGCTTCTTTTACACTGTCATCATCTCGTTGATGTTAGGAACAATTTGTTGGAGATTTGGAGCTAAGAGGGATACACAGCAGGATTTATTAAATGCCATGGGATCCATGTATGCAGCAATCCTATTTAGCGGAATCACCAATGCCACTGCTGTTCAACCTGTTGTTTCTGTAGAACGTTTTGTTTCATATCGAGAGAGAGCTGCAGGGATGTACTCAGCTTTACCATTTGCATTTGCTCAGGTGGTGATAGAGCTCCCTTATGTGTTTGCACAAGCAATTTTCTACTGCACAATTTTTTACTCCACGGCCTCATTTGAGTGGACTGCTTTGAAGTTTCTTTGGTACATATTCTTTATGTACTTCACAATGCTGTACTTCACCTTCTACGGAATGATGACAACCGCTGTCACCCCAAATCATAACGTGGCCGCTGTAATTGCTGCTCCATTTTATATGCTGTGGAACCTCTTCAGCGGCTTTATGATTCCTCACAAGAGAATTCCAATATGGTGGAGATGGTATTACTGGGCAAACCCGGTAGCCTGGAGTCTGTACGGCCTCTTTGTTTCACAGTACGGCGAGGATGACAGTCTTTTGACACTAGCTGACGGAATCCATAAAATGCCTGTGAGGCAGTTACTCAAAGTAGGGTTTGGTTATAAGCATGATTTTCTGGGTGTAGCCGGCATTATGGTGGTCGGTTTCTGCGTGTTTTTCGCCTTTATTTTTGCCTTCGCAATAAAATCGTTCAACTTCCAGAGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006855 GO:0007275 GO:0008150 GO:0015399 GO:0015405 GO:0015893 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031224 GO:0032501 GO:0032502 GO:0042221 GO:0042335 GO:0042493 GO:0042623 GO:0042626 GO:0043492 GO:0044425 GO:0044464 GO:0048856 GO:0050896 GO:0051179 GO:0051234 GO:0055085 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ABC2_membrane ABC_tran ABC_trans_N PDR_assoc

Protein Analysis

1420

Amino Acids

161.64

Weight (kDa)

8.41

Isoelectric Point (pI)

41.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_trans_N	PF14510	73 - 126	1.2e-06	ABC-transporter N-terminal
ABC_tran	PF00005	151 - 332	7.9e-15	ABC transporter
ABC2_membrane	PF01061	488 - 699	4e-44	ABC-2 type transporter
PDR_assoc	PF08370	704 - 767	8.2e-27	Plant PDR ABC transporter associated
ABC_tran	PF00005	845 - 997	3.2e-18	ABC transporter
ABC2_membrane	PF01061	1142 - 1356	5.3e-55	ABC-2 type transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0008072)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26910
fragaria_vesca	FvH4_1g04210 FvH4_1g04210 FvH4_1g04210 FvH4_1g04210 FvH4_1g04210 FvH4_1g04210 FvH4_1g04210
malus_domestica	MD02G1040300.v1.1 MD15G1145300.v1.1
prunus_persica	Prupe.7G235300_v2.0.a1
pyrus_communis	pycom02g03360 pycom15g16260
rosa_chinensis	RchiOBHm_Chr2g0089741
rosa_laevigata	RLG00000016052
rosa_multiflora	Rmu_sc0000665.1_g000015
rosa_roxburghii	Rroxscaffold_2G00151650
rosa_rugosa	Rorug02G0002800 Rorug02G0002900
rosa_samantha	Rh2AG048100 Rh2BG046900 Rh2CG049000 Rh2DG048300
rosa_wichuraiana	Rw2G004230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	4152
AatII	GACGTC	1 cut(s)	168
Acc36I	ACCTGC	1 cut(s)	1108
AccB1I	GGYRCC	1 cut(s)	549
AccBSI	CCGCTC	1 cut(s)	94
AccI	GTMKAC	3 cut(s)	2020, 2887, 2909
AccII	CGCG	4 cut(s)	29, 86, 123, 1176
AccIII	TCCGGA	1 cut(s)	515
AclI	AACGTT	1 cut(s)	3667
AclWI	GGATC	6 cut(s)	1832, 1917, 1930, 1969, 3588, 3601
AcoI	YGGCCR	1 cut(s)	3918
AcsI	RAATTY	5 cut(s)	286, 1780, 2093, 2706, 3990
AcuI	CTGAAG	2 cut(s)	1395, 3946
AcyI	GRCGYC	2 cut(s)	165, 550
AfeI	AGCGCT	2 cut(s)	72, 357
AfiI	CCNNNNNNNGG	7 cut(s)	405, 515, 844, 1741, 1918, 2870, 2990
AflII	CTTAAG	2 cut(s)	604, 1768
AflIII	ACRYGT	1 cut(s)	21
AgeI	ACCGGT	1 cut(s)	560
AhlI	ACTAGT	1 cut(s)	2848
AjiI	CACGTC	1 cut(s)	343
AjnI	CCWGG	6 cut(s)	552, 1760, 2560, 2757, 3226, 4034
AjuI	GAANNNNNNNTTGG	4 cut(s)	3361, 3393, 3523, 3555
AloI	GAACNNNNNNTCC	2 cut(s)	8, 40
Alw21I	GWGCWC	8 cut(s)	177, 1066, 2167, 2430, 2859, 3117, 3144, 3747
Alw26I	GTCTC	6 cut(s)	141, 824, 1103, 1437, 2126, 2359
AlwI	GGATC	6 cut(s)	1832, 1917, 1930, 1969, 3588, 3601
Ama87I	CYCGRG	4 cut(s)	111, 176, 236, 1059
Aor13HI	TCCGGA	1 cut(s)	515
Aor51HI	AGCGCT	2 cut(s)	72, 357
ApoI	RAATTY	5 cut(s)	286, 1780, 2093, 2706, 3990
AsiGI	ACCGGT	1 cut(s)	560
Asp700I	GAANNNNTTC	5 cut(s)	23, 2313, 2766, 3429, 3959
AspLEI	GCGC	9 cut(s)	73, 88, 123, 261, 358, 552, 767, 1628, 3374
AsuC2I	CCSGG	3 cut(s)	547, 2892, 4028
AsuHPI	GGTGA	6 cut(s)	2293, 2851, 3619, 3748, 3862, 3892
AsuII	TTCGAA	1 cut(s)	3380
AvaI	CYCGRG	4 cut(s)	111, 176, 236, 1059
AvaII	GGWCC	8 cut(s)	304, 503, 968, 1169, 1270, 1287, 3155, 3164
BaeI	ACNNNNGTAYC	2 cut(s)	879, 912
BamHI	GGATCC	2 cut(s)	1922, 3593
BanI	GGYRCC	1 cut(s)	549
BanII	GRGCYC	5 cut(s)	177, 2859, 3117, 3144, 3747
BarI	GAAGNNNNNNTAC	2 cut(s)	3857, 3889
BbsI	GAAGAC	1 cut(s)	1370
Bbv12I	GWGCWC	8 cut(s)	177, 1066, 2167, 2430, 2859, 3117, 3144, 3747
BbvCI	CCTCAGC	1 cut(s)	621
BccI	CCATC	6 cut(s)	1419, 1601, 2803, 2836, 2894, 4002
BceAI	ACGGC	5 cut(s)	134, 3185, 3813, 4063, 4084
BcgI	CGANNNNNNTGC	4 cut(s)	1040, 1074, 4218, 4252
BciT130I	CCWGG	6 cut(s)	554, 1762, 2562, 2759, 3228, 4036
BciVI	GTATCC	2 cut(s)	1991, 3556
BcnI	CCSGG	3 cut(s)	547, 2892, 4028
BcoDI	GTCTC	6 cut(s)	141, 824, 1103, 1437, 2126, 2359
BcuI	ACTAGT	1 cut(s)	2848
BfaI	CTAG	8 cut(s)	1382, 1737, 1851, 2261, 2849, 3084, 3287, 4094
BfmI	CTRYAG	8 cut(s)	948, 1416, 2910, 3009, 3267, 3486, 3660, 3693
BfoI	RGCGCY	4 cut(s)	74, 359, 553, 3375
BfrI	CTTAAG	2 cut(s)	604, 1768
BfuAI	ACCTGC	1 cut(s)	1108
BfuI	GTATCC	2 cut(s)	1991, 3556
BglI	GCCNNNNNGGC	1 cut(s)	4123
BglII	AGATCT	1 cut(s)	787
BmcAI	AGTACT	4 cut(s)	1283, 2372, 3191, 3407
Bme1390I	CCNGG	9 cut(s)	547, 554, 1762, 2562, 2759, 2892, 3228, 4028, 4036
Bme18I	GGWCC	8 cut(s)	304, 503, 968, 1169, 1270, 1287, 3155, 3164
BmeT110I	CYCGRG	4 cut(s)	111, 176, 236, 1059
BmgBI	CACGTC	1 cut(s)	343
BmrFI	CCNGG	9 cut(s)	547, 554, 1762, 2562, 2759, 2892, 3228, 4028, 4036
BmrI	ACTGGG	1 cut(s)	4027
BmsI	GCATC	4 cut(s)	1060, 1327, 2989, 3240
BmuI	ACTGGG	1 cut(s)	4027
BpiI	GAAGAC	1 cut(s)	1370
BplI	GAGNNNNNCTC	2 cut(s)	3126, 3158
BpmI	CTGGAG	4 cut(s)	110, 1089, 2568, 4057
Bpu10I	CCTNAGC	4 cut(s)	183, 621, 3359, 3729
Bpu14I	TTCGAA	1 cut(s)	3380
BpuEI	CTTGAG	2 cut(s)	710, 896
BpuMI	CCSGG	3 cut(s)	547, 2892, 4028
BsaAI	YACGTR	2 cut(s)	588, 661
BsaBI	GATNNNNATC	2 cut(s)	786, 1341
BsaHI	GRCGYC	2 cut(s)	165, 550
BsaI	GGTCTC	2 cut(s)	1103, 2126
BsaWI	WCCGGW	4 cut(s)	515, 560, 1575, 1699
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 2334, 2364
Bsc4I	CCNNNNNNNGG	7 cut(s)	405, 515, 844, 1741, 1918, 2870, 2990
Bse118I	RCCGGY	4 cut(s)	207, 560, 1321, 4175
Bse1I	ACTGG	9 cut(s)	1009, 1289, 1484, 2195, 2551, 2644, 3055, 3470, 4022
Bse3DI	GCAATG	2 cut(s)	1583, 2153
Bse8I	GATNNNNATC	2 cut(s)	786, 1341
BseAI	TCCGGA	1 cut(s)	515
BseBI	CCWGG	6 cut(s)	554, 1762, 2562, 2759, 3228, 4036
BseGI	GGATG	9 cut(s)	997, 1411, 2623, 2905, 2983, 3004, 3255, 3705, 4081
BseJI	GATNNNNATC	2 cut(s)	786, 1341
BseLI	CCNNNNNNNGG	7 cut(s)	405, 515, 844, 1741, 1918, 2870, 2990
BseMI	GCAATG	2 cut(s)	1583, 2153
BseMII	CTCAG	7 cut(s)	635, 936, 1214, 2354, 3491, 3720, 3743
BseNI	ACTGG	9 cut(s)	1009, 1289, 1484, 2195, 2551, 2644, 3055, 3470, 4022
BseRI	GAGGAG	1 cut(s)	3150
BseYI	CCCAGC	3 cut(s)	767, 1358, 1740
BsgI	GTGCAG	2 cut(s)	2616, 3763
Bsh1236I	CGCG	4 cut(s)	29, 86, 123, 1176
Bsh1285I	CGRYCG	2 cut(s)	208, 253
BshNI	GGYRCC	1 cut(s)	549
BshTI	ACCGGT	1 cut(s)	560
BsiEI	CGRYCG	2 cut(s)	208, 253
BsiHKAI	GWGCWC	8 cut(s)	177, 1066, 2167, 2430, 2859, 3117, 3144, 3747
BsiHKCI	CYCGRG	4 cut(s)	111, 176, 236, 1059
BslFI	GGGAC	7 cut(s)	71, 602, 1182, 1733, 2468, 2659, 3150
BslI	CCNNNNNNNGG	7 cut(s)	405, 515, 844, 1741, 1918, 2870, 2990
BsmAI	GTCTC	6 cut(s)	141, 824, 1103, 1437, 2126, 2359
BsmBI	CGTCTC	1 cut(s)	1437
BsmFI	GGGAC	7 cut(s)	71, 602, 1182, 1733, 2468, 2659, 3150
BsmI	GAATGC	4 cut(s)	1051, 2380, 2508, 2661
Bso31I	GGTCTC	2 cut(s)	1103, 2126
BsoBI	CYCGRG	4 cut(s)	111, 176, 236, 1059
Bsp119I	TTCGAA	1 cut(s)	3380
Bsp1286I	GDGCHC	8 cut(s)	177, 1066, 2167, 2430, 2859, 3117, 3144, 3747
Bsp13I	TCCGGA	1 cut(s)	515
Bsp19I	CCATGG	3 cut(s)	153, 1641, 3588
Bsp68I	TCGCGA	1 cut(s)	29
BspCNI	CTCAG	7 cut(s)	634, 935, 1215, 2355, 3490, 3719, 3742
BspEI	TCCGGA	1 cut(s)	515
BspFNI	CGCG	4 cut(s)	29, 86, 123, 1176
BspHI	TCATGA	2 cut(s)	801, 855
BspMAI	CTGCAG	4 cut(s)	1420, 3013, 3490, 3697
BspMI	ACCTGC	1 cut(s)	1108
BspPI	GGATC	6 cut(s)	1832, 1917, 1930, 1969, 3588, 3601
BspQI	GCTCTTC	2 cut(s)	2252, 2304
BspT104I	TTCGAA	1 cut(s)	3380
BspT107I	GGYRCC	1 cut(s)	549
BspTI	CTTAAG	2 cut(s)	604, 1768
BspTNI	GGTCTC	2 cut(s)	1103, 2126
BsrBI	CCGCTC	1 cut(s)	94
BsrDI	GCAATG	2 cut(s)	1583, 2153
BsrFI	RCCGGY	4 cut(s)	207, 560, 1321, 4175
BsrI	ACTGG	9 cut(s)	1009, 1289, 1484, 2195, 2551, 2644, 3055, 3470, 4022
BssAI	RCCGGY	4 cut(s)	207, 560, 1321, 4175
BssNAI	GTATAC	1 cut(s)	2021
BssNI	GRCGYC	2 cut(s)	165, 550
BssT1I	CCWWGG	7 cut(s)	153, 1641, 1926, 2098, 2277, 2304, 3588
Bst1107I	GTATAC	1 cut(s)	2021
Bst2UI	CCWGG	6 cut(s)	554, 1762, 2562, 2759, 3228, 4036
Bst6I	CTCTTC	9 cut(s)	748, 1442, 1913, 2252, 2304, 2511, 2777, 2829, 3965
BstACI	GRCGYC	2 cut(s)	165, 550
BstAFI	CTTAAG	2 cut(s)	604, 1768
BstAPI	GCANNNNNTGC	2 cut(s)	1070, 3008
BstBAI	YACGTR	2 cut(s)	588, 661
BstBI	TTCGAA	1 cut(s)	3380
BstC8I	GCNNGC	5 cut(s)	173, 257, 2167, 3365, 4177
BstDSI	CCRYGG	4 cut(s)	153, 1641, 3588, 3795
BstEII	GGTNACC	1 cut(s)	2790
BstF5I	GGATG	9 cut(s)	997, 1411, 2623, 2905, 2983, 3004, 3255, 3705, 4081
BstFNI	CGCG	4 cut(s)	29, 86, 123, 1176
BstH2I	RGCGCY	4 cut(s)	74, 359, 553, 3375
BstHHI	GCGC	9 cut(s)	73, 88, 123, 261, 358, 552, 767, 1628, 3374
BstMAI	GTCTC	6 cut(s)	141, 824, 1103, 1437, 2126, 2359
BstMCI	CGRYCG	2 cut(s)	208, 253
BstNI	CCWGG	6 cut(s)	554, 1762, 2562, 2759, 3228, 4036
BstNSI	RCATGY	2 cut(s)	259, 2245
BstPI	GGTNACC	1 cut(s)	2790
BstSCI	CCNGG	9 cut(s)	545, 552, 1760, 2560, 2757, 2890, 3226, 4026, 4034
BstSFI	CTRYAG	8 cut(s)	948, 1416, 2910, 3009, 3267, 3486, 3660, 3693
BstUI	CGCG	4 cut(s)	29, 86, 123, 1176
BstV2I	GAAGAC	1 cut(s)	1370
BstX2I	RGATCY	4 cut(s)	787, 1922, 1961, 3593
BstXI	CCANNNNNNTGG	3 cut(s)	2031, 2614, 4002
BstYI	RGATCY	4 cut(s)	787, 1922, 1961, 3593
BstZ17I	GTATAC	1 cut(s)	2021
BsuI	GTATCC	2 cut(s)	1991, 3556
BtgI	CCRYGG	4 cut(s)	153, 1641, 3588, 3795
BtrI	CACGTC	1 cut(s)	343
BtsCI	GGATG	9 cut(s)	997, 1411, 2623, 2905, 2983, 3004, 3255, 3705, 4081
BtsI	GCAGTG	2 cut(s)	3483, 3636
BtsIMutI	CAGTG	6 cut(s)	1077, 1220, 2544, 3483, 3504, 3636
BtuMI	TCGCGA	1 cut(s)	29
BveI	ACCTGC	1 cut(s)	1108
Cac8I	GCNNGC	5 cut(s)	173, 257, 2167, 3365, 4177
CciI	TCATGA	2 cut(s)	801, 855
CfoI	GCGC	9 cut(s)	73, 88, 123, 261, 358, 552, 767, 1628, 3374
Cfr10I	RCCGGY	4 cut(s)	207, 560, 1321, 4175
CseI	GACGC	1 cut(s)	281
CspAI	ACCGGT	1 cut(s)	560
CspCI	CAANNNNNGTGG	2 cut(s)	822, 857
DinI	GGCGCC	1 cut(s)	551
EaeI	YGGCCR	1 cut(s)	3918
Eam1104I	CTCTTC	9 cut(s)	748, 1442, 1913, 2252, 2304, 2511, 2777, 2829, 3965
EarI	CTCTTC	9 cut(s)	748, 1442, 1913, 2252, 2304, 2511, 2777, 2829, 3965
EciI	GGCGGA	3 cut(s)	29, 407, 3484
Ecl136II	GAGCTC	5 cut(s)	175, 2857, 3115, 3142, 3745
Eco130I	CCWWGG	7 cut(s)	153, 1641, 1926, 2098, 2277, 2304, 3588
Eco147I	AGGCCT	3 cut(s)	1614, 2560, 3226
Eco24I	GRGCYC	5 cut(s)	177, 2859, 3117, 3144, 3747
Eco31I	GGTCTC	2 cut(s)	1103, 2126
Eco47I	GGWCC	8 cut(s)	304, 503, 968, 1169, 1270, 1287, 3155, 3164
Eco47III	AGCGCT	2 cut(s)	72, 357
Eco53kI	GAGCTC	5 cut(s)	175, 2857, 3115, 3142, 3745
Eco57I	CTGAAG	2 cut(s)	1395, 3946
Eco88I	CYCGRG	4 cut(s)	111, 176, 236, 1059
Eco91I	GGTNACC	1 cut(s)	2790
EcoICRI	GAGCTC	5 cut(s)	175, 2857, 3115, 3142, 3745
EcoO109I	RGGNCCY	2 cut(s)	397, 1169
EcoO65I	GGTNACC	1 cut(s)	2790
EcoRI	GAATTC	2 cut(s)	2093, 3990
EcoRII	CCWGG	6 cut(s)	552, 1760, 2560, 2757, 3226, 4034
EcoT14I	CCWWGG	7 cut(s)	153, 1641, 1926, 2098, 2277, 2304, 3588
EcoT22I	ATGCAT	1 cut(s)	1510
EcoT38I	GRGCYC	5 cut(s)	177, 2859, 3117, 3144, 3747
EgeI	GGCGCC	1 cut(s)	551
EheI	GGCGCC	1 cut(s)	551
ErhI	CCWWGG	7 cut(s)	153, 1641, 1926, 2098, 2277, 2304, 3588
Esp3I	CGTCTC	1 cut(s)	1437
FaqI	GGGAC	7 cut(s)	71, 602, 1182, 1733, 2468, 2659, 3150
FauI	CCCGC	2 cut(s)	458, 1181
FauNDI	CATATG	1 cut(s)	878
FblI	GTMKAC	3 cut(s)	2020, 2887, 2909
FokI	GGATG	9 cut(s)	1004, 1398, 2630, 2912, 2990, 3011, 3262, 3712, 4088
FriOI	GRGCYC	5 cut(s)	177, 2859, 3117, 3144, 3747
FspBI	CTAG	8 cut(s)	1382, 1737, 1851, 2261, 2849, 3084, 3287, 4094
GlaI	GCGC	9 cut(s)	72, 87, 122, 260, 357, 551, 766, 1627, 3373
GsaI	CCCAGC	3 cut(s)	771, 1362, 1744
GsuI	CTGGAG	4 cut(s)	110, 1089, 2568, 4057
HaeII	RGCGCY	4 cut(s)	74, 359, 553, 3375
HgaI	GACGC	1 cut(s)	281
HhaI	GCGC	9 cut(s)	73, 88, 123, 261, 358, 552, 767, 1628, 3374
Hin1I	GRCGYC	2 cut(s)	165, 550
Hin6I	GCGC	9 cut(s)	71, 86, 121, 259, 356, 550, 765, 1626, 3372
HinP1I	GCGC	9 cut(s)	71, 86, 121, 259, 356, 550, 765, 1626, 3372
HincII	GTYRAC	2 cut(s)	444, 1581
HindII	GTYRAC	2 cut(s)	444, 1581
HindIII	AAGCTT	2 cut(s)	330, 1694
HpaI	GTTAAC	1 cut(s)	444
HphI	GGTGA	6 cut(s)	2293, 2851, 3619, 3748, 3862, 3892
Hpy99I	CGWCG	8 cut(s)	143, 146, 167, 230, 233, 347, 2480, 3206
Hsp92I	GRCGYC	2 cut(s)	165, 550
HspAI	GCGC	9 cut(s)	71, 86, 121, 259, 356, 550, 765, 1626, 3372
KasI	GGCGCC	1 cut(s)	549
KflI	GGGWCCC	1 cut(s)	1169
Kpn2I	TCCGGA	1 cut(s)	515
KroI	GCCGGC	1 cut(s)	4175
KroNI	GCCGGC	1 cut(s)	4177
KspAI	GTTAAC	1 cut(s)	444
LguI	GCTCTTC	2 cut(s)	2252, 2304
LweI	GCATC	4 cut(s)	1060, 1327, 2989, 3240
MaeI	CTAG	8 cut(s)	1382, 1737, 1851, 2261, 2849, 3084, 3287, 4094
MaeIII	GTNAC	9 cut(s)	583, 1555, 2299, 2542, 2714, 2790, 3259, 3898, 4128
MbiI	CCGCTC	1 cut(s)	94
MfeI	CAATTG	2 cut(s)	410, 1599
MflI	RGATCY	4 cut(s)	787, 1922, 1961, 3593
MhlI	GDGCHC	8 cut(s)	177, 1066, 2167, 2430, 2859, 3117, 3144, 3747
Mly113I	GGCGCC	1 cut(s)	550
MlyI	GAGTC	3 cut(s)	1802, 2396, 4048
Mph1103I	ATGCAT	1 cut(s)	1510
MroI	TCCGGA	1 cut(s)	515
MroNI	GCCGGC	1 cut(s)	4175
MroXI	GAANNNNTTC	5 cut(s)	23, 2313, 2766, 3429, 3959
MslI	CAYNNNNRTG	5 cut(s)	1673, 2612, 3248, 3808, 4184
MspA1I	CMGCKG	5 cut(s)	625, 2530, 3896, 3923, 3966
MspCI	CTTAAG	2 cut(s)	604, 1768
MspR9I	CCNGG	9 cut(s)	547, 554, 1762, 2562, 2759, 2892, 3228, 4028, 4036
MunI	CAATTG	2 cut(s)	410, 1599
Mva1269I	GAATGC	4 cut(s)	1051, 2380, 2508, 2661
MvaI	CCWGG	6 cut(s)	554, 1762, 2562, 2759, 3228, 4036
MvnI	CGCG	4 cut(s)	29, 86, 123, 1176
NaeI	GCCGGC	1 cut(s)	4177
NarI	GGCGCC	1 cut(s)	550
NciI	CCSGG	3 cut(s)	547, 2892, 4028
NcoI	CCATGG	3 cut(s)	153, 1641, 3588
NdeI	CATATG	1 cut(s)	878
NgoMIV	GCCGGC	1 cut(s)	4175
NmeAIII	GCCGAG	1 cut(s)	1602
NmuCI	GTSAC	5 cut(s)	583, 1555, 2299, 2542, 3898
NruI	TCGCGA	1 cut(s)	29
NsiI	ATGCAT	1 cut(s)	1510
NspI	RCATGY	2 cut(s)	259, 2245
NspV	TTCGAA	1 cut(s)	3380
PaeI	GCATGC	1 cut(s)	259
PaeR7I	CTCGAG	3 cut(s)	111, 176, 1059
PagI	TCATGA	2 cut(s)	801, 855
PceI	AGGCCT	3 cut(s)	1614, 2560, 3226
PciSI	GCTCTTC	2 cut(s)	2252, 2304
PcsI	WCGNNNNNNNCGW	4 cut(s)	168, 234, 259, 4197
PctI	GAATGC	4 cut(s)	1051, 2380, 2508, 2661
PdiI	GCCGGC	1 cut(s)	4177
PdmI	GAANNNNTTC	5 cut(s)	23, 2313, 2766, 3429, 3959
PfeI	GAWTC	7 cut(s)	918, 1854, 2762, 3101, 3624, 3976, 4104
PflFI	GACNNNGTC	2 cut(s)	705, 1369
PfoI	TCCNGGA	1 cut(s)	2757
PinAI	ACCGGT	1 cut(s)	560
Ple19I	CGATCG	1 cut(s)	253
PleI	GAGTC	3 cut(s)	1801, 2395, 4047
PluTI	GGCGCC	1 cut(s)	553
PpsI	GAGTC	3 cut(s)	1801, 2395, 4047
Ppu21I	YACGTR	2 cut(s)	588, 661
PpuMI	RGGWCCY	1 cut(s)	1169
PsiI	TTATAA	1 cut(s)	4152
Psp124BI	GAGCTC	5 cut(s)	177, 2859, 3117, 3144, 3747
Psp1406I	AACGTT	1 cut(s)	3667
Psp5II	RGGWCCY	1 cut(s)	1169
Psp6I	CCWGG	6 cut(s)	552, 1760, 2560, 2757, 3226, 4034
PspEI	GGTNACC	1 cut(s)	2790
PspFI	CCCAGC	3 cut(s)	767, 1358, 1740
PspGI	CCWGG	6 cut(s)	552, 1760, 2560, 2757, 3226, 4034
PspPPI	RGGWCCY	1 cut(s)	1169
PspXI	VCTCGAGB	3 cut(s)	111, 176, 1059
PsrI	GAACNNNNNNTAC	2 cut(s)	3464, 3496
PstI	CTGCAG	4 cut(s)	1420, 3013, 3490, 3697
PsuI	RGATCY	4 cut(s)	787, 1922, 1961, 3593
PsyI	GACNNNGTC	2 cut(s)	705, 1369
PvuI	CGATCG	1 cut(s)	253
PvuII	CAGCTG	1 cut(s)	2530
RruI	TCGCGA	1 cut(s)	29
RseI	CAYNNNNRTG	5 cut(s)	1673, 2612, 3248, 3808, 4184
SacI	GAGCTC	5 cut(s)	177, 2859, 3117, 3144, 3747
SapI	GCTCTTC	2 cut(s)	2252, 2304
ScaI	AGTACT	4 cut(s)	1283, 2372, 3191, 3407
SchI	GAGTC	3 cut(s)	1802, 2396, 4048
ScrFI	CCNGG	9 cut(s)	547, 554, 1762, 2562, 2759, 2892, 3228, 4028, 4036
SduI	GDGCHC	8 cut(s)	177, 1066, 2167, 2430, 2859, 3117, 3144, 3747
SfaNI	GCATC	4 cut(s)	1060, 1327, 2989, 3240
SfcI	CTRYAG	8 cut(s)	948, 1416, 2910, 3009, 3267, 3486, 3660, 3693
SfoI	GGCGCC	1 cut(s)	551
Sfr274I	CTCGAG	3 cut(s)	111, 176, 1059
SfuI	TTCGAA	1 cut(s)	3380
SinI	GGWCC	8 cut(s)	304, 503, 968, 1169, 1270, 1287, 3155, 3164
SlaI	CTCGAG	3 cut(s)	111, 176, 1059
SmiMI	CAYNNNNRTG	5 cut(s)	1673, 2612, 3248, 3808, 4184
SmlI	CTYRAG	7 cut(s)	111, 176, 604, 689, 911, 1059, 1768
SmoI	CTYRAG	7 cut(s)	111, 176, 604, 689, 911, 1059, 1768
SpeI	ACTAGT	1 cut(s)	2848
SphI	GCATGC	1 cut(s)	259
SseBI	AGGCCT	3 cut(s)	1614, 2560, 3226
SspDI	GGCGCC	1 cut(s)	549
SspI	AATATT	1 cut(s)	2405
SspMI	CTAG	8 cut(s)	1382, 1737, 1851, 2261, 2849, 3084, 3287, 4094
SstI	GAGCTC	5 cut(s)	177, 2859, 3117, 3144, 3747
StuI	AGGCCT	3 cut(s)	1614, 2560, 3226
StyD4I	CCNGG	9 cut(s)	545, 552, 1760, 2560, 2757, 2890, 3226, 4026, 4034
StyI	CCWWGG	7 cut(s)	153, 1641, 1926, 2098, 2277, 2304, 3588
TatI	WGTACW	9 cut(s)	849, 1281, 2370, 3189, 3405, 3480, 3702, 3849, 3864
TauI	GCSGC	6 cut(s)	86, 97, 126, 943, 3923, 3969
TfiI	GAWTC	7 cut(s)	918, 1854, 2762, 3101, 3624, 3976, 4104
TscAI	CASTG	6 cut(s)	1084, 1227, 2551, 3490, 3511, 3643
TseFI	GTSAC	5 cut(s)	583, 1555, 2299, 2542, 3898
Tsp45I	GTSAC	5 cut(s)	583, 1555, 2299, 2542, 3898
TspGWI	ACGGA	6 cut(s)	609, 1746, 1901, 2074, 3894, 4116
TspRI	CASTG	6 cut(s)	1084, 1227, 2551, 3490, 3511, 3643
Tth111I	GACNNNGTC	2 cut(s)	705, 1369
Vha464I	CTTAAG	2 cut(s)	604, 1768
VpaK11BI	GGWCC	8 cut(s)	304, 503, 968, 1169, 1270, 1287, 3155, 3164
XapI	RAATTY	5 cut(s)	286, 1780, 2093, 2706, 3990
XceI	RCATGY	2 cut(s)	259, 2245
XcmI	CCANNNNNNNNNTGG	2 cut(s)	532, 2187
XhoI	CTCGAG	3 cut(s)	111, 176, 1059
XmiI	GTMKAC	3 cut(s)	2020, 2887, 2909
XmnI	GAANNNNTTC	5 cut(s)	23, 2313, 2766, 3429, 3959
XspI	CTAG	8 cut(s)	1382, 1737, 1851, 2261, 2849, 3084, 3287, 4094
ZraI	GACGTC	1 cut(s)	166
ZrmI	AGTACT	4 cut(s)	1283, 2372, 3191, 3407
Zsp2I	ATGCAT	1 cut(s)	1510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.