Rh2AG048100
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0008072
Physical Location & Seq
Reverse (-)
3785047 .. 3792751
7705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG048100.1

Sequence Viewer

Length: 3996 bp
ATGTACAGAGTAGATTTGGAGTTTCCGAAGATCGAGGTGCGGTTTCAGAATTTGAACGTTGAAGCTTTCGTCCATGTGGGAAGCAGAGCACTGCCAACTATTCCCAATTTCGTATTCAATATGACCGAGGCACTTCTGAGGCAATTACGGCTATTAAGGAGCAAGAGAAGCAAATTAACGATTTTAGACAACATTAGTGGGATTATAAGACCTTCAAGATTGACGTTGTTATTGGGTCCACCCAGCTCCGGCAAGACGACGCTACTATTGGCTCTCGCCGGACGCCTTGGAACTGGTCTTCAGATGTCAGGTAGCACAACATACAATGGCCATGGCTTAAACGAGTTTGTGCCGCAGAGGACGTCGGCTTATGTCAGTCAACAGGACTGGTTTGCGGCGGAGATGACTGTAAGGGAGACTCTTGACTTTGCAGGACGGTGTCAAGGTGTTGGATTCAAATATGATATGCTAGTAGAACTAGCAAGAAGAGAAAAGATTGCGGGGATCAAACCTGATGGTGATCTTGATATATTCATGAAGTCATTAGCTCTTGGAGAGCAAGAGACAAGCCTTGTTGTAGAGTACATCATGAAGATTTTAGGGTTGGACATATGCGCTGATACGTTGGTAGGAGACGAAATGCTTAAAGGTATATCAGGGGGCCAAAAGAAGCGGCTTACTACAGGTGAATTACTAGTAGGTCCAGCAAGAGTGTTGTTCATGGATGAAATATCAACTGGGCTCGATAGTTCCACTACTTATCAAATCATCAAGTATCTTAGGCATTCAACTCGTGCACTTGATTCCACCACTGTTATATCTCTGCTTCAACCTGCTCCAGAGACCTACGAGTTGTTTGATGATGTTATACTTTTGTGTGAGGGCCAGATAGTGTTTCAGGGACCCCGGGAATCTGCACTTGATTTCTTTTCATACATGGGGTTTAGATGTCCCCGCCGAAAGAATGTGGCAGACTTCTTGCAAGAAGTTATATCAAAGAAGGACCAAGAGCAGTACTGGTCCAATCCTGATCTTCCATACCGCTATATACCACCAGCAAAGTTTGTTGAAGCTTTTCCTTCATTTCAAGATGGGAAGAATTTGTCTGCAGAGTTGAAAGTTCCTTTTGATAAACGCTATAACCACCCTGCAGCTTTGGCGACTTCTCTCTATGGCATGAAGAGGAAGGAACTTCTCAAGACAAGCTTTAACTGGCAAGTGCTACTGATGAAGCGGAATGCATTTATTTATATTTTCAAATTTGTTCAGCTTCTTTTGGTTGCTTTGATCACAATGAGTGTCTTCTGCCGGACAAAAATGCACCATGATACAATTGATGATGGAGGCTTATATCTCGGGGCACTATACTTTTCCATGGTTATTATTCTTTTTAATGGCTTTACGGAGGTGCCAATGTTGGTGGCCAAGCTTCCAGTGCTTTACAAGCATAGGGACTTACACTTCTACCCAAGTTGGGTCTATACTTTTCCTTCCTGGCTCTTGAGTATTCCAAATTCACTCATAGAGTCTGGACTTTGGGTGGCAATCACATACTACGTCATTGGATATGATCCCTCAATTTCTAGATTTTTTGGGCAGTTCTTGGTATATTTCCTTCTGCACCAGATGTCCACAGCTCTCTTTCGTCTTATGGGATCCTTGGGTCGAAATATGATAGTTGCCAACACCTTTGGATCTTTTGCCATGTTGGTTGTAATGGCTCTTGGAGGATACATCGTCTCTAGAGATCATATACCAAAATGGTGGATTTGGGGTTTCTGGTTTTCTCCTTTGATGTATGCTCAAAATGCTGCTTCTGTAAATGAATTCCTTGGACACTCCTGGAACACGGAACATGGGCATGAGACCGGTCTTTCATTAGGTCAGTCATTACTAAAAGCACGCAGTTTGTTTCCAGAGAGATACTGGTATTGGATTGGTGCTGGTGCTTTGCTTGGCTATACAGTTTTATTCAATTTACTATTCACATTCTTCCTTGCTTACCTCAACCCCTTGGGGAAGCAACAAGCTGTTGTGTCTAAGGAAGAGCTGGAAGAGAGAGAAAGGAGAAGAAAAGGAGAAAGTGTTGTTATTGAGCTGAGACAGTACTTGAATCATTCACAGTCACTTAATGGAAAATACTTTAAGCAGAGAGGAATGGTCCTCCCATTTCAGCCACTTTCCATGTCTTTCAGCAATATCAATTACTACGTTGATGTCCCTCTGGAATTGAAGCAGCAGGGAATTCAAGAAGAGAGATTGCAGCTCTTGGTTGATGTCACTGGAGCATTTAGGCCGGGGGTGCTTACAGCATTGGTTGGAGTAAGTGGTGCTGGTAAAACCACCCTCATGGATGTATTGGCTGGTCGAAAAACTGGTGGGGTTATAGAAGGAAGCATACATATATCTGGTCATCCAAAAAGACAAGAAACTTTTGCAAGAATTTCGGGTTACTGCGAGCAATCTGATATTCACTCCCCGTGCTTGACTGTTGTTGAATCGCTTCTGTTCTCTGTTTGGCTTCGCTTGCCATCAGATGTTGACTTGGATACACGGAAGGCTTTTGTAGAGGAGGTGATGGAACTTGTGGAGCTCACTCCATTAAGTGGGGCACTGGTTGGTTTACCGGGGGTGAATGGCTTGTCAACAGAACAACGAAAAAGGCTAACTATAGCAGTGGAGCTGGTCGCTAATCCATCTATAGTATTCATGGATGAACCCACATCTGGGTTAGATGCAAGGTCTGCAGCCATTGTGATGAGGACTGTGAGGAATATAGTCGATACTGGACGTACTATCGTATGCACAATCCACCAGCCTAGCATAGACATTTTTGAATCCTTTGATGAGCTTCTGTTTTTGAAGCGTGGCGGTCAGCTCATATATGCTGGTCCACTTGGACCCAACTCTTGTGAACTCATCAAGTACTTTGAGGCAATTGAAGGAGTGCAAAAGATCAGGCCTGGATATAATCCTGCTGCATGGATGCTTGATGTTACTTCTTCAACTGAAGAAATCCGTCTGGGGCTGGATTTTGCAGAAATTTACAGAAGATCAAATTTGTTTCAACAAAACAAAGACTTGGTTGAAAACCTAAGCAAGCCGAGTACTAATTCCAAAGAATTAAACTTTCCAACCAAATACTCTCAGACATCATTTGAACAATTTCTCACTTGTCTATGGAAGCAAAATCTGTCTTATTGGCGAAACCCTCAGTACACTGCAGTTCGTTTCTTTTACACTGTCATCATCTCCTTGATGCTAGGAACAATTTGTTGGAGATTCGGGGCTAAGAGGGAGACCCAGCAGGACTTATTAAATGCCATGGGATCCTTGTATGCAGCAATCCTATTTAGTGGAGTCACCAATGCCACTGCTGTTCAACCTGTTGTTTCTATAGAAAGATTTGTCTCATATCGAGAGAGAGCTGCTGGGATGTACTCGGCGTTACCATTTGCATTTGCTCAGGTTGCAATAGAGTTCCCTTATGTGTTTGCGCAAGCGGCCATCTACTGCACAATTTTCTACTCCACGGCTGCATTTGACTGGACACTTTTAAAGTTTGTTTGGTACTTATTCTTCATGTATTTCACAATGCTATACTTCACCTTTTACGGAATGATGACAACAGCCGTCACACCAAATCATAACGTAGCTGCCATAATTGCTGCTCCATTTTACATGCTATGGAATCTTTTCAGCGGCTTCATGATTCCTCACAAGAAAATTCCCGTATGGTGGAGATGGTATTACTGGGCAAACCCTGTAGCCTGGAGTCTGTATGGCCTGTTTGTTTCGCAGTACGGTGACGATGACAGTCTTTTGACGCTAGCAGATGGAGAAAGAACAATGCCGGTAAGGCAGTTCCTCAAAGTTGGTTTTGGGTACAGGCATGATTTCCTAGGTGTAGCCGGAATTATGGTCGTCGGTTTCTGCATACTTTTCGCAATAATTTTTGCCTATGCAATAAAATCATTCAACTTCCAGAGGAGATGA

Protein Analysis

1331

Amino Acids

151.21

Weight (kDa)

8.66

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 62 - 243 1.7e-14 ABC transporter
ABC2_membrane PF01061 399 - 610 1.4e-48 ABC-2 type transporter
PDR_assoc PF08370 615 - 679 8.7e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 759 - 909 9.1e-19 ABC transporter
ABC2_membrane PF01061 1054 - 1268 7e-55 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 206
AatII GACGTC 1 cut(s) 365
Acc16I TGCGCA 1 cut(s) 3498
Acc36I ACCTGC 1 cut(s) 841
AccB1I GGYRCC 1 cut(s) 1408
AccB7I CCANNNNNTGG 1 cut(s) 2606
AclI AACGTT 1 cut(s) 57
AclWI GGATC 7 cut(s) 512, 1565, 1650, 1663, 1702, 3324, 3337
AcoI YGGCCR 3 cut(s) 328, 1422, 3504
AcuI CTGAAG 2 cut(s) 284, 3030
AcyI GRCGYC 2 cut(s) 283, 362
AfiI CCNNNNNNNGG 6 cut(s) 248, 1474, 2606, 2726, 2727, 3738
AgeI ACCGGT 1 cut(s) 1868
AhlI ACTAGT 1 cut(s) 694
AjnI CCWGG 4 cut(s) 1493, 1841, 2962, 3770
AjuI GAANNNNNNNTTGG 4 cut(s) 98, 130, 3259, 3291
Alw21I GWGCWC 3 cut(s) 91, 799, 2595
Alw26I GTCTC 9 cut(s) 410, 557, 627, 836, 1744, 1859, 2095, 3293, 3415
Alw44I GTGCAC 1 cut(s) 795
AlwI GGATC 7 cut(s) 512, 1565, 1650, 1663, 1702, 3324, 3337
Ama87I CYCGRG 2 cut(s) 906, 1355
AoxI GGCC 8 cut(s) 328, 661, 883, 1422, 2294, 2960, 3504, 3784
ApaLI GTGCAC 1 cut(s) 795
AsiGI ACCGGT 1 cut(s) 1868
Asp700I GAANNNNTTC 4 cut(s) 1074, 2502, 3165, 3695
AspA2I CCTAGG 1 cut(s) 3901
AspLEI GCGC 2 cut(s) 617, 3499
AsuC2I CCSGG 4 cut(s) 907, 908, 2298, 2628
AsuHPI GGTGA 7 cut(s) 530, 698, 2587, 2644, 3355, 3598, 3818
AsuNHI GCTAGC 1 cut(s) 3829
AvaI CYCGRG 2 cut(s) 906, 1355
AvaII GGWCC 8 cut(s) 236, 701, 902, 1003, 1020, 2161, 2891, 2900
AvrII CCTAGG 1 cut(s) 3901
BaeGI GKGCMC 3 cut(s) 799, 1363, 2614
BaeI ACNNNNGTAYC 2 cut(s) 612, 645
BalI TGGCCA 2 cut(s) 330, 1424
BamHI GGATCC 2 cut(s) 1655, 3329
BanI GGYRCC 1 cut(s) 1408
BanII GRGCYC 2 cut(s) 744, 2595
BarI GAAGNNNNNNTAC 6 cut(s) 1599, 1631, 2382, 2414, 3563, 3595
BauI CACGAG 1 cut(s) 792
BbsI GAAGAC 2 cut(s) 290, 1294
Bbv12I GWGCWC 3 cut(s) 91, 799, 2595
BccI CCATC 9 cut(s) 509, 1085, 1334, 2539, 2572, 2704, 3515, 3738, 3830
BceAI ACGGC 3 cut(s) 164, 3549, 3617
BcgI CGANNNNNNTGC 6 cut(s) 773, 807, 2427, 2461, 3925, 3959
BciT130I CCWGG 4 cut(s) 1495, 1843, 2964, 3772
BciVI GTATCC 2 cut(s) 1724, 2542
BclI TGATCA 1 cut(s) 1287
BcnI CCSGG 4 cut(s) 907, 908, 2298, 2628
BcoDI GTCTC 9 cut(s) 410, 557, 627, 836, 1744, 1859, 2095, 3293, 3415
BcuI ACTAGT 1 cut(s) 694
BfaI CTAG 9 cut(s) 470, 479, 695, 1584, 1743, 2820, 3263, 3830, 3902
BfmI CTRYAG 9 cut(s) 681, 1107, 1149, 2670, 2700, 2745, 3222, 3396, 3765
BfuAI ACCTGC 1 cut(s) 841
BfuI GTATCC 2 cut(s) 1724, 2542
BglI GCCNNNNNGGC 1 cut(s) 3859
BlnI CCTAGG 1 cut(s) 3901
BmcAI AGTACT 4 cut(s) 1016, 2108, 2927, 3109
Bme1390I CCNGG 8 cut(s) 907, 908, 1495, 1843, 2298, 2628, 2964, 3772
Bme18I GGWCC 8 cut(s) 236, 701, 902, 1003, 1020, 2161, 2891, 2900
BmeT110I CYCGRG 2 cut(s) 906, 1355
BmiI GGNNCC 8 cut(s) 237, 662, 903, 904, 1410, 1657, 2902, 3331
BmrFI CCNGG 8 cut(s) 907, 908, 1495, 1843, 2298, 2628, 2964, 3772
BmrI ACTGGG 2 cut(s) 747, 3763
BmsI GCATC 3 cut(s) 2725, 2976, 3249
BmtI GCTAGC 1 cut(s) 3833
BmuI ACTGGG 2 cut(s) 747, 3763
BpiI GAAGAC 2 cut(s) 290, 1294
BpmI CTGGAG 3 cut(s) 822, 2304, 3793
Bpu10I CCTNAGC 2 cut(s) 3095, 3465
BpuEI CTTGAG 2 cut(s) 1181, 1522
BpuMI CCSGG 4 cut(s) 907, 908, 2298, 2628
BsaBI GATNNNNATC 1 cut(s) 519
BsaHI GRCGYC 2 cut(s) 283, 362
BsaI GGTCTC 3 cut(s) 836, 1859, 3293
BsaWI WCCGGW 1 cut(s) 1868
BsaXI ACNNNNNCTCC 2 cut(s) 2070, 2100
Bsc4I CCNNNNNNNGG 6 cut(s) 248, 1474, 2606, 2726, 2727, 3738
Bse118I RCCGGY 2 cut(s) 1868, 3853
Bse8I GATNNNNATC 1 cut(s) 519
BseBI CCWGG 4 cut(s) 1495, 1843, 2964, 3772
BseGI GGATG 6 cut(s) 730, 2359, 2413, 2719, 2991, 3441
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 6 cut(s) 248, 1474, 2606, 2726, 2727, 3738
BseMII CTCAG 5 cut(s) 128, 2090, 3161, 3227, 3479
BseRI GAGGAG 1 cut(s) 2585
BseSI GKGCMC 3 cut(s) 799, 1363, 2614
BseYI CCCAGC 3 cut(s) 242, 3303, 3431
BsgI GTGCAG 3 cut(s) 900, 1604, 3499
BshFI GGCC 8 cut(s) 330, 663, 885, 1424, 2296, 2962, 3506, 3786
BshNI GGYRCC 1 cut(s) 1408
BshTI ACCGGT 1 cut(s) 1868
BsiHKAI GWGCWC 3 cut(s) 91, 799, 2595
BsiHKCI CYCGRG 2 cut(s) 906, 1355
BsiSI CCGG 9 cut(s) 249, 279, 907, 1309, 1869, 2297, 2627, 3854, 3912
BslFI GGGAC 4 cut(s) 915, 936, 1466, 2204
BslI CCNNNNNNNGG 6 cut(s) 248, 1474, 2606, 2726, 2727, 3738
BsmAI GTCTC 9 cut(s) 410, 557, 627, 836, 1744, 1859, 2095, 3293, 3415
BsmBI CGTCTC 2 cut(s) 627, 1744
BsmFI GGGAC 4 cut(s) 915, 936, 1466, 2204
BsmI GAATGC 2 cut(s) 784, 1243
BsnI GGCC 8 cut(s) 330, 663, 885, 1424, 2296, 2962, 3506, 3786
Bso31I GGTCTC 3 cut(s) 836, 1859, 3293
BsoBI CYCGRG 2 cut(s) 906, 1355
Bsp1286I GDGCHC 6 cut(s) 91, 744, 799, 1363, 2595, 2614
Bsp1407I TGTACA 1 cut(s) 3
Bsp19I CCATGG 3 cut(s) 331, 1374, 3324
BspANI GGCC 8 cut(s) 330, 663, 885, 1424, 2296, 2962, 3506, 3786
BspCNI CTCAG 5 cut(s) 129, 2091, 3160, 3226, 3478
BspHI TCATGA 3 cut(s) 534, 588, 3708
BspLI GGNNCC 8 cut(s) 237, 662, 903, 904, 1410, 1657, 2902, 3331
BspMAI CTGCAG 4 cut(s) 1111, 1153, 2749, 3226
BspMI ACCTGC 1 cut(s) 841
BspOI GCTAGC 1 cut(s) 3833
BspPI GGATC 7 cut(s) 512, 1565, 1650, 1663, 1702, 3324, 3337
BspQI GCTCTTC 1 cut(s) 2040
BspT107I GGYRCC 1 cut(s) 1408
BspTNI GGTCTC 3 cut(s) 836, 1859, 3293
BsrFI RCCGGY 2 cut(s) 1868, 3853
BsrGI TGTACA 1 cut(s) 3
BssAI RCCGGY 2 cut(s) 1868, 3853
BssNI GRCGYC 2 cut(s) 283, 362
BssSI CACGAG 1 cut(s) 792
BssT1I CCWWGG 8 cut(s) 286, 331, 1374, 1659, 1831, 2013, 3324, 3901
Bst2BI CACGAG 1 cut(s) 792
Bst2UI CCWGG 4 cut(s) 1495, 1843, 2964, 3772
Bst6I CTCTTC 5 cut(s) 481, 1175, 2040, 2049, 2247
BstACI GRCGYC 2 cut(s) 283, 362
BstAPI GCANNNNNTGC 1 cut(s) 2744
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 6 cut(s) 1903, 2459, 2528, 3101, 3501, 3831
BstDEI CTNAG 9 cut(s) 137, 779, 2040, 2099, 3095, 3147, 3213, 3291, 3465
BstDSI CCRYGG 4 cut(s) 331, 1374, 3324, 3531
BstF5I GGATG 6 cut(s) 730, 2359, 2413, 2719, 2991, 3441
BstHHI GCGC 2 cut(s) 617, 3499
BstMAI GTCTC 9 cut(s) 410, 557, 627, 836, 1744, 1859, 2095, 3293, 3415
BstNI CCWGG 4 cut(s) 1495, 1843, 2964, 3772
BstNSI RCATGY 1 cut(s) 3685
BstSCI CCNGG 8 cut(s) 905, 906, 1493, 1841, 2296, 2626, 2962, 3770
BstSFI CTRYAG 9 cut(s) 681, 1107, 1149, 2670, 2700, 2745, 3222, 3396, 3765
BstSLI GKGCMC 3 cut(s) 799, 1363, 2614
BstV2I GAAGAC 2 cut(s) 290, 1294
BstX2I RGATCY 3 cut(s) 1655, 1694, 3329
BstXI CCANNNNNNTGG 2 cut(s) 1764, 2350
BstYI RGATCY 3 cut(s) 1655, 1694, 3329
BsuI GTATCC 2 cut(s) 1724, 2542
BsuRI GGCC 8 cut(s) 330, 663, 885, 1424, 2296, 2962, 3506, 3786
BtgI CCRYGG 4 cut(s) 331, 1374, 3324, 3531
BtsCI GGATG 6 cut(s) 730, 2359, 2413, 2719, 2991, 3441
BtsI GCAGTG 4 cut(s) 89, 2682, 3219, 3372
BtsIMutI CAGTG 9 cut(s) 89, 810, 1440, 2280, 2612, 2682, 3219, 3240, 3372
BveI ACCTGC 1 cut(s) 841
Cac8I GCNNGC 6 cut(s) 1903, 2459, 2528, 3101, 3501, 3831
CciI TCATGA 3 cut(s) 534, 588, 3708
CfoI GCGC 2 cut(s) 617, 3499
Cfr10I RCCGGY 2 cut(s) 1868, 3853
Cfr9I CCCGGG 1 cut(s) 906
CseI GACGC 3 cut(s) 268, 291, 3835
CspAI ACCGGT 1 cut(s) 1868
CspCI CAANNNNNGTGG 4 cut(s) 178, 213, 1401, 1436
DdeI CTNAG 9 cut(s) 137, 779, 2040, 2099, 3095, 3147, 3213, 3291, 3465
DraI TTTAAA 1 cut(s) 3558
EaeI YGGCCR 3 cut(s) 328, 1422, 3504
Eam1104I CTCTTC 5 cut(s) 481, 1175, 2040, 2049, 2247
EarI CTCTTC 5 cut(s) 481, 1175, 2040, 2049, 2247
EciI GGCGGA 1 cut(s) 413
Ecl136II GAGCTC 1 cut(s) 2593
Eco130I CCWWGG 8 cut(s) 286, 331, 1374, 1659, 1831, 2013, 3324, 3901
Eco147I AGGCCT 1 cut(s) 2962
Eco24I GRGCYC 2 cut(s) 744, 2595
Eco31I GGTCTC 3 cut(s) 836, 1859, 3293
Eco47I GGWCC 8 cut(s) 236, 701, 902, 1003, 1020, 2161, 2891, 2900
Eco53kI GAGCTC 1 cut(s) 2593
Eco57I CTGAAG 2 cut(s) 284, 3030
Eco88I CYCGRG 2 cut(s) 906, 1355
EcoICRI GAGCTC 1 cut(s) 2593
EcoO109I RGGNCCY 1 cut(s) 902
EcoRI GAATTC 2 cut(s) 1826, 2244
EcoRII CCWGG 4 cut(s) 1493, 1841, 2962, 3770
EcoT14I CCWWGG 8 cut(s) 286, 331, 1374, 1659, 1831, 2013, 3324, 3901
EcoT22I ATGCAT 1 cut(s) 1243
EcoT38I GRGCYC 2 cut(s) 744, 2595
ErhI CCWWGG 8 cut(s) 286, 331, 1374, 1659, 1831, 2013, 3324, 3901
Esp3I CGTCTC 2 cut(s) 627, 1744
FalI AAGNNNNNCTT 2 cut(s) 1190, 1222
FaqI GGGAC 4 cut(s) 915, 936, 1466, 2204
FauI CCCGC 2 cut(s) 493, 962
FauNDI CATATG 1 cut(s) 611
FbaI TGATCA 1 cut(s) 1287
FokI GGATG 6 cut(s) 737, 2366, 2400, 2726, 2998, 3448
FriOI GRGCYC 2 cut(s) 744, 2595
FspBI CTAG 9 cut(s) 470, 479, 695, 1584, 1743, 2820, 3263, 3830, 3902
FspI TGCGCA 1 cut(s) 3498
GlaI GCGC 2 cut(s) 616, 3498
GsaI CCCAGC 3 cut(s) 246, 3307, 3435
GsuI CTGGAG 3 cut(s) 822, 2304, 3793
HaeIII GGCC 8 cut(s) 330, 663, 885, 1424, 2296, 2962, 3506, 3786
HapII CCGG 9 cut(s) 249, 279, 907, 1309, 1869, 2297, 2627, 3854, 3912
HgaI GACGC 3 cut(s) 268, 291, 3835
HhaI GCGC 2 cut(s) 617, 3499
Hin1I GRCGYC 2 cut(s) 283, 362
Hin6I GCGC 2 cut(s) 615, 3497
HinP1I GCGC 2 cut(s) 615, 3497
HincII GTYRAC 3 cut(s) 380, 2542, 2646
HindII GTYRAC 3 cut(s) 380, 2542, 2646
HindIII AAGCTT 4 cut(s) 63, 1071, 1204, 1427
HpaII CCGG 9 cut(s) 249, 279, 907, 1309, 1869, 2297, 2627, 3854, 3912
HphI GGTGA 7 cut(s) 530, 698, 2587, 2644, 3355, 3598, 3818
Hpy188I TCNGA 7 cut(s) 27, 48, 138, 303, 2467, 2536, 3150
Hpy99I CGWCG 3 cut(s) 262, 367, 3929
HpyAV CCTTC 9 cut(s) 222, 994, 1089, 1180, 1500, 1625, 2384, 2551, 2936
HpyCH4IV ACGT 8 cut(s) 57, 224, 362, 623, 1557, 2211, 2791, 3651
HpyF3I CTNAG 9 cut(s) 137, 779, 2040, 2099, 3095, 3147, 3213, 3291, 3465
HpySE526I ACGT 8 cut(s) 57, 224, 362, 623, 1557, 2211, 2791, 3651
Hsp92I GRCGYC 2 cut(s) 283, 362
HspAI GCGC 2 cut(s) 615, 3497
KflI GGGWCCC 1 cut(s) 902
Ksp22I TGATCA 1 cut(s) 1287
LguI GCTCTTC 1 cut(s) 2040
LmnI GCTCC 7 cut(s) 159, 251, 841, 2285, 2590, 2680, 3676
LweI GCATC 3 cut(s) 2725, 2976, 3249
MaeI CTAG 9 cut(s) 470, 479, 695, 1584, 1743, 2820, 3263, 3830, 3902
MaeII ACGT 8 cut(s) 57, 224, 362, 623, 1557, 2211, 2791, 3651
MaeIII GTNAC 8 cut(s) 2124, 2278, 2450, 2995, 3361, 3447, 3634, 3806
MfeI CAATTG 2 cut(s) 1332, 2937
MflI RGATCY 3 cut(s) 1655, 1694, 3329
MhlI GDGCHC 6 cut(s) 91, 744, 799, 1363, 2595, 2614
MlsI TGGCCA 2 cut(s) 330, 1424
MluNI TGGCCA 2 cut(s) 330, 1424
MlyI GAGTC 4 cut(s) 412, 1535, 3369, 3784
MmeI TCCRAC 5 cut(s) 430, 585, 2299, 3158, 3257
Mox20I TGGCCA 2 cut(s) 330, 1424
Mph1103I ATGCAT 1 cut(s) 1243
MroXI GAANNNNTTC 4 cut(s) 1074, 2502, 3165, 3695
MscI TGGCCA 2 cut(s) 330, 1424
MslI CAYNNNNRTG 3 cut(s) 1860, 2348, 2756
Msp20I TGGCCA 2 cut(s) 330, 1424
MspA1I CMGCKG 1 cut(s) 3702
MspI CCGG 9 cut(s) 249, 279, 907, 1309, 1869, 2297, 2627, 3854, 3912
MspR9I CCNGG 8 cut(s) 907, 908, 1495, 1843, 2298, 2628, 2964, 3772
MunI CAATTG 2 cut(s) 1332, 2937
Mva1269I GAATGC 2 cut(s) 784, 1243
MvaI CCWGG 4 cut(s) 1495, 1843, 2964, 3772
NciI CCSGG 4 cut(s) 907, 908, 2298, 2628
NcoI CCATGG 3 cut(s) 331, 1374, 3324
NdeI CATATG 1 cut(s) 611
NheI GCTAGC 1 cut(s) 3829
NlaIV GGNNCC 8 cut(s) 237, 662, 903, 904, 1410, 1657, 2902, 3331
NmeAIII GCCGAG 2 cut(s) 3129, 3422
NmuCI GTSAC 5 cut(s) 2124, 2278, 3361, 3634, 3806
NsbI TGCGCA 1 cut(s) 3498
NsiI ATGCAT 1 cut(s) 1243
NspI RCATGY 1 cut(s) 3685
PagI TCATGA 3 cut(s) 534, 588, 3708
PceI AGGCCT 1 cut(s) 2962
PciSI GCTCTTC 1 cut(s) 2040
PctI GAATGC 2 cut(s) 784, 1243
PdmI GAANNNNTTC 4 cut(s) 1074, 2502, 3165, 3695
PfeI GAWTC 9 cut(s) 453, 803, 911, 2113, 2498, 2837, 3282, 3691, 3712
PflFI GACNNNGTC 1 cut(s) 438
PflMI CCANNNNNTGG 1 cut(s) 2606
PfoI TCCNGGA 1 cut(s) 1841
PinAI ACCGGT 1 cut(s) 1868
PleI GAGTC 4 cut(s) 412, 1534, 3368, 3783
PpsI GAGTC 4 cut(s) 412, 1534, 3368, 3783
PpuMI RGGWCCY 1 cut(s) 902
PsiI TTATAA 1 cut(s) 206
Psp124BI GAGCTC 1 cut(s) 2595
Psp1406I AACGTT 1 cut(s) 57
Psp5II RGGWCCY 1 cut(s) 902
Psp6I CCWGG 4 cut(s) 1493, 1841, 2962, 3770
PspFI CCCAGC 3 cut(s) 242, 3303, 3431
PspGI CCWGG 4 cut(s) 1493, 1841, 2962, 3770
PspN4I GGNNCC 8 cut(s) 237, 662, 903, 904, 1410, 1657, 2902, 3331
PspPPI RGGWCCY 1 cut(s) 902
PstI CTGCAG 4 cut(s) 1111, 1153, 2749, 3226
PsuI RGATCY 3 cut(s) 1655, 1694, 3329
PsyI GACNNNGTC 1 cut(s) 438
RseI CAYNNNNRTG 3 cut(s) 1860, 2348, 2756
SacI GAGCTC 1 cut(s) 2595
SapI GCTCTTC 1 cut(s) 2040
ScaI AGTACT 4 cut(s) 1016, 2108, 2927, 3109
SchI GAGTC 4 cut(s) 412, 1535, 3369, 3784
ScrFI CCNGG 8 cut(s) 907, 908, 1495, 1843, 2298, 2628, 2964, 3772
SduI GDGCHC 6 cut(s) 91, 744, 799, 1363, 2595, 2614
SfaNI GCATC 3 cut(s) 2725, 2976, 3249
SfcI CTRYAG 9 cut(s) 681, 1107, 1149, 2670, 2700, 2745, 3222, 3396, 3765
SinI GGWCC 8 cut(s) 236, 701, 902, 1003, 1020, 2161, 2891, 2900
SmaI CCCGGG 1 cut(s) 908
SmiMI CAYNNNNRTG 3 cut(s) 1860, 2348, 2756
SmlI CTYRAG 2 cut(s) 1196, 1501
SmoI CTYRAG 2 cut(s) 1196, 1501
SpeI ACTAGT 1 cut(s) 694
SseBI AGGCCT 1 cut(s) 2962
SspMI CTAG 9 cut(s) 470, 479, 695, 1584, 1743, 2820, 3263, 3830, 3902
SstI GAGCTC 1 cut(s) 2595
StuI AGGCCT 1 cut(s) 2962
StyD4I CCNGG 8 cut(s) 905, 906, 1493, 1841, 2296, 2626, 2962, 3770
StyI CCWWGG 8 cut(s) 286, 331, 1374, 1659, 1831, 2013, 3324, 3901
TaiI ACGT 8 cut(s) 60, 227, 365, 626, 1560, 2214, 2794, 3654
TaqI TCGA 6 cut(s) 33, 744, 1666, 2368, 2781, 3418
TaqII GACCGA 1 cut(s) 140
TatI WGTACW 8 cut(s) 3, 582, 1014, 2106, 2925, 3107, 3216, 3438
TauI GCSGC 5 cut(s) 355, 398, 676, 3506, 3705
TfiI GAWTC 9 cut(s) 453, 803, 911, 2113, 2498, 2837, 3282, 3691, 3712
TscAI CASTG 9 cut(s) 96, 817, 1440, 2287, 2619, 2682, 3226, 3247, 3379
TseFI GTSAC 5 cut(s) 2124, 2278, 3361, 3634, 3806
Tsp45I GTSAC 5 cut(s) 2124, 2278, 3361, 3634, 3806
TspGWI ACGGA 5 cut(s) 1418, 1865, 2569, 3008, 3630
TspMI CCCGGG 1 cut(s) 906
TspRI CASTG 9 cut(s) 96, 817, 1440, 2287, 2619, 2682, 3226, 3247, 3379
Tth111I GACNNNGTC 1 cut(s) 438
Van91I CCANNNNNTGG 1 cut(s) 2606
VneI GTGCAC 1 cut(s) 795
VpaK11BI GGWCC 8 cut(s) 236, 701, 902, 1003, 1020, 2161, 2891, 2900
XbaI TCTAGA 2 cut(s) 1583, 1742
XceI RCATGY 1 cut(s) 3685
XcmI CCANNNNNNNNNTGG 1 cut(s) 1923
XmaI CCCGGG 1 cut(s) 906
XmaJI CCTAGG 1 cut(s) 3901
XmnI GAANNNNTTC 4 cut(s) 1074, 2502, 3165, 3695
XspI CTAG 9 cut(s) 470, 479, 695, 1584, 1743, 2820, 3263, 3830, 3902
ZraI GACGTC 1 cut(s) 363
ZrmI AGTACT 4 cut(s) 1016, 2108, 2927, 3109
Zsp2I ATGCAT 1 cut(s) 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.