RchiOBHm_Chr2g0089741
ERF Family

Belongs to the ABC transporter superfamily. ABCG family. PDR (TC 3.A.1.205) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008072
Physical Location & Seq
Reverse (-)
3834055 .. 3841543
7489 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46504

Sequence Viewer

Length: 4266 bp
ATGTGGAACTCGGCGGAGAACGCGTTTGCGAGGACGGCGTCGTTTCGGGTGGAGGGGGAGGACGAGGAGGCGCTGCGGTGGGCGGCGCTGGAGAGGCTGCCGACATACTCCAGAGTCCGACGTGGCATTTTCAAGAACATCGTGGGAGACGCCAAGGAGGTTGACGTCAGCGAGCTGGAGGCTCAGGAGCTGAAGCTACTGCTTGATCGGCTTGTTAACTCGGCTGAAGATGACCCCGAGCACTTCTTTGAGAAAATGCGACTCCGATTTAACGCAGTAGATTTGGAGTTTCCGAAGATCGAGGTGCGGTTTCAGAAATTGAACGTTGAAGCTTTCGTCCATGTGGGAAGCAGAGCACTGCCAACTATTCCGAATTTCGTATTCAATATGACCGAGGTATCACTTCTGAGGCAATTACGGCTATTAAGGAGCAAGAGAAGCAAATTAACGATTTTAGACAACATTAGTGGGATTATAAGACCTTCAAGATTGACGTTGTTATTGGGTCCGCCGAGCTCCGGCAAGACGACGCTACTATTGGCTCTCGCCGGACGCCTTGGAACTGGTCTTCAGATGTCAGGTAGCACAACATACAATGGCCATGGCTTAAACGAGTTTGTGCCGCAGAGGACGTCGGCTTATGTCAGTCAACAGGACTGGTTTGCGGCGGAGATGACTGTAAGGGAGACTCTTGACTTTGCAGGACGGTGTCAAGGTGTTGGATTCAAATATGATATGCTAGTAGAACTAGCAAGAAGAGAAAAGATTGCGGGGATAAAACCTGATGGTGATCTTGATATATTCATGAAGTCATTAGCTCTTGGAGAGCAAGAGACAAGCCTTGTTGTAGAGTACATCATGAAGATTTTAGGGTTGGACATATGCGCTGATACGTTGGTAGGAGACGAAATGCTTAAAGGTATATCAGGGGGCCAAAAGAAGCGGCTTACTACAGGTGAATTACTAGTAGGTCCAGCAAGAGTGTTGTTCATGGATGAAATATCAACTGGGCTCGATAGTTCCACTACTTATCAAATAATCAAATATCTTAGGCATTCAACTCGTGCACTTGATTCCACCACTGTTATATCTCTGCTTCAACCTGCTCCAGAGACCTACGAGTTGTTTGATGATGTTATACTTTTGTGTGAGGGCCAGATAGTGTTTCAGGGACCCCGGGAATCTGCACTTGATTTCTTTTCATACATGGGGTTTAGATGTCCCCGCCGAAAGAATGTGGCAGACTTCTTGCAAGAAGTTATATCAAAGAAGGACCAAGAGCAGTACTGGTCCAATCCTGATCTTCCATACCGCTATATACCACCAGCAAAGTTTGTTGAAGCTTTTCCTTCATTTCAAGATGGGAAGAATTTGTCTGCAGAGTTGAAAGTTCCTTTTGATAAACGCTATAACCACCCTGCAGCTTTGGCGACTTCTCTCTATGGCATGAAGAGGAAGGAGCTTCTGAAGACAAGCTTTAACTGGCAAATGCTACTGATGAAGCGGAATGCATTTATTTATATTTTCAAATTTGTTCAGCTTCTTTTGGTTGCTTTGATCACAATGAGTGTCTTCTGCCGGACAAAAATGCACCATGATACAATTGATGATGGAGGCTTATATCTCGGGGCACTATACTTTTCCATGGTTATTATTCTTTTTAATGGCTTTACGGAGGTGCCAATGTTGGTGGCCAAGCTTCCAGTGCTTTACAAGCATAGGGACTTACACTTCTACCCAAGTTGGGTCTATACTCTTCCTTCCTGGCTCTTGAGTATTCCAAATTCACTCATAGAGTCTGGACTTTGGGTGGCAATCACATACTACGTCATTGGATATGATCCCGCAATTTCTAGATTTTTTGGGCAGTTCTTGGTATATTTCCTTCTGCACCAGATGTCCACAGCTCTCTTTCGTCTTATGGGATCCTTGGGTCGAAATATGATAGTTGCCAACACCTTTGGATCTTTTGCCATGTTGGTTGTAATGGCTCTTGGAGGATACATCGTCTCTAGAGATCATATACCAAAATGGTGGATTTGGGGTTTCTGGTTTTCTCCTTTGATGTATGCTCAAAATGCTGCTTCTGTAAATGAATTCCTTGGACACTCCTGGAACACGGAACATGGGCATGAGACCGGTCTTTCATTAGGTCAGTCATTACTAAAAGCACGCAGTTTGTTTCCAGAGAGATACTGGTATTGGATTGGTGCTGGTGCTTTGCTTGGCTATACAGTTTTATTCAATTTACTATTCACATTCTTCCTTGCTTACCTCAACCCCTTGGGGAAGCAACAAGCTGTTGTGTCTAAGGAAGAGCTGGAAGAGAGAGAAAGGAGAAGAAAAGGAGAAAGTGTTGTTATTGAGCTGAGACAGTACTTGAATCATTCACAGTCACTTAATGGAAAATACTTTAAGCAGAGAGGAATGGTCCTCCCATTTCAGCCACTTTCCATGTCTTTCAGCAATATCAATTACTACGTCGATGTCCCTCTGGAATTGAAGCAGCAGGGAATTCAAGAAGAGAGATTGCAGCTCTTGGTTGATGTCACTGGAGCATTTAGGCCGGGGGTGCTTACAGCATTGGTTGGAGTCAGTGGTGCTGGTAAAACCACCCTCATGGACGTATTGGCTGGTCGAAAAACTGGTGGGGTTATAGAAGGAAGCATACATATATCTGGTCATCCAAAAAGACAAGAAACTTTTGCAAGAATTTCGGGTTACTGCGAGCAATCTGATATTCACTCCCCGTGCTTGACTGTTGTTGAATCGCTTCTGTTCTCTGTTTGGCTTCGCTTGCCATCAGATGTTAACTTGGATACACGGAAGGCTTTTGTAGAGGAGGTGATGGAACTTGTGGAGCTCACTCCATTAAGTGGGGCACTGGTTGGTTTACCGGGGGTGAATGGCTTGTCAACAGAACAACGAAAAAGGCTAACTATAGCAGTGGAGCTGGTCGCTAATCCATCTATAGTATTCATGGATGAACCCACATCTGGGTTAGATGCAAGGTCTGCAGCCATTGTGATGAGGACTGTGAGGAATATAGTTGATACTGGACGTACTATCGTATGCACAATCCACCAGCCTAGCATAGACATTTTTGAATCCTTTGATGAGCTTCTGTTTTTGAAGCGTGGCGGTCAGCTCATATATGCTGGTCCACTTGGACCCAACTCTTGTGAACTCATCAAGTACTTTGAGGCAATTGAAGGAGTGCAAAAGATCAGGCCTGGATATAATCCTGCTGCATGGATGCTTGATGTTACTTCTTCAACTGAAGAAATCCGTCTGGGGGTGGATTTTGCAGAAATTTACAGAAGATCAAATTTGTTTCAACATAACAAAGATTTGGTTGAAAACCTTAGCAAGCCGAGTACTAATTCCAAAGAATTAAACTTTCCAACCAAATACTCTCAGACATCATTTGAACAATTTCTCACTTGTCTATGGAAGCAAAATCTGTCTTATTGGCGAAACCCTCAGTACACTGCAGTTCGTTTCTTTTACACCGTCATCATCTCCTTGATGCTAGGAACAATTTGTTGGAGATTCGGGGCTAAGAGGGAGACCCAGCAGGACTTATTAAATGCCATGGGATCCTTGTATGCAGCAATCCTATTTAGTGGAGTCACCAATGCCACTGCTGTTCAACCTGTTGTTTCTATAGAAAGATTTGTCTCATATCGAGAGAGAGCTGCTGGGATGTACTCGGCGTTACCATTTGCATTTGCTCAGGTTGCAATAGAGTTCCCTTATGTGTTTGCGCAAGCGGTCATCTACTGCACAATTTTCTACTCCACGGCTGCATTTGACTGGACACTTTTAAAGTTTGTTTGGTACTTATTCTTCATGTATTTCACAATGCTATACTTCACCTTTTACGGAATGATGACAACAGCCGTCACACCAAATCATAACGTAGCTGCCATAATTGCTGCTCCATTTTACATGCTATGGAATCTTTTCAGCGGCTTCATGATTCCTCACAAGAGAATTCCCGTATGGTGGAGATGGTATTACTGGGCAAACCCTGTAGCCTGGAGTCTGTATGGCCTGTTTGTTTCGCAGTACGGTGACGATGACAGTCTTTTGAAGCTAGCAGATGGAGAAAGAACAATGCCAGTAAGGCAGTTCCTCAAAGTTGGTTTTGGGTACAGGCATGATTTCCTAGGTGTAGCCGGAATTATGGTCTTCGGTTTCTGCATACTTTTCGCAATAATTTTTGCCTATGCAATAAAATCATTCAACTTCCAGAGGAGATGA

Protein Analysis

1421

Amino Acids

161.69

Weight (kDa)

8.45

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_trans_N PF14510 73 - 126 1.3e-06 ABC-transporter N-terminal
ABC_tran PF00005 152 - 333 1.9e-14 ABC transporter
ABC2_membrane PF01061 489 - 700 3.3e-48 ABC-2 type transporter
PDR_assoc PF08370 705 - 769 9.4e-27 Plant PDR ABC transporter associated
ABC_tran PF00005 849 - 999 9.9e-19 ABC transporter
ABC2_membrane PF01061 1144 - 1358 9e-55 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
AatII GACGTC 2 cut(s) 168, 635
Acc16I TGCGCA 1 cut(s) 3768
Acc36I ACCTGC 1 cut(s) 1111
AccB1I GGYRCC 1 cut(s) 1678
AccB7I CCANNNNNTGG 1 cut(s) 2876
AccII CGCG 1 cut(s) 23
AclI AACGTT 1 cut(s) 324
AclWI GGATC 6 cut(s) 1835, 1920, 1933, 1972, 3594, 3607
AcoI YGGCCR 2 cut(s) 598, 1692
AcuI CTGAAG 5 cut(s) 212, 246, 554, 1487, 3300
AcyI GRCGYC 5 cut(s) 38, 150, 165, 553, 632
AfiI CCNNNNNNNGG 7 cut(s) 518, 1744, 2876, 2996, 2997, 3295, 4008
AflIII ACRYGT 1 cut(s) 21
AgeI ACCGGT 1 cut(s) 2138
AhlI ACTAGT 1 cut(s) 964
AjiI CACGTC 1 cut(s) 122
AjnI CCWGG 4 cut(s) 1763, 2111, 3232, 4040
AjuI GAANNNNNNNTTGG 2 cut(s) 3529, 3561
Alw21I GWGCWC 5 cut(s) 243, 358, 518, 1069, 2865
Alw44I GTGCAC 1 cut(s) 1065
AlwI GGATC 6 cut(s) 1835, 1920, 1933, 1972, 3594, 3607
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 3 cut(s) 236, 1176, 1625
AoxI GGCC 7 cut(s) 598, 931, 1153, 1692, 2564, 3230, 4054
ApaLI GTGCAC 1 cut(s) 1065
AsiGI ACCGGT 1 cut(s) 2138
Asp700I GAANNNNTTC 4 cut(s) 1344, 2772, 3435, 3965
AspA2I CCTAGG 1 cut(s) 4171
AspLEI GCGC 4 cut(s) 73, 88, 887, 3769
AsuC2I CCSGG 4 cut(s) 1177, 1178, 2568, 2898
AsuHPI GGTGA 7 cut(s) 800, 968, 2857, 2914, 3625, 3868, 4088
AsuNHI GCTAGC 1 cut(s) 4099
AvaI CYCGRG 3 cut(s) 236, 1176, 1625
AvaII GGWCC 8 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170
AvrII CCTAGG 1 cut(s) 4171
BaeGI GKGCMC 3 cut(s) 1069, 1633, 2884
BaeI ACNNNNGTAYC 4 cut(s) 381, 414, 882, 915
BalI TGGCCA 2 cut(s) 600, 1694
BamHI GGATCC 2 cut(s) 1925, 3599
BanI GGYRCC 1 cut(s) 1678
BanII GRGCYC 3 cut(s) 518, 1014, 2865
BarI GAAGNNNNNNTAC 6 cut(s) 1869, 1901, 2652, 2684, 3833, 3865
BauI CACGAG 1 cut(s) 1062
BbsI GAAGAC 4 cut(s) 560, 1475, 1564, 4186
Bbv12I GWGCWC 5 cut(s) 243, 358, 518, 1069, 2865
BccI CCATC 8 cut(s) 779, 1355, 1604, 2809, 2842, 2974, 4008, 4100
BceAI ACGGC 4 cut(s) 51, 434, 3819, 3887
BcgI CGANNNNNNTGC 6 cut(s) 1043, 1077, 2697, 2731, 4195, 4229
BciT130I CCWGG 4 cut(s) 1765, 2113, 3234, 4042
BciVI GTATCC 2 cut(s) 1994, 2812
BclI TGATCA 1 cut(s) 1557
BcnI CCSGG 4 cut(s) 1177, 1178, 2568, 2898
BcuI ACTAGT 1 cut(s) 964
BfaI CTAG 9 cut(s) 740, 749, 965, 1854, 2013, 3090, 3533, 4100, 4172
BfmI CTRYAG 9 cut(s) 951, 1377, 1419, 2940, 2970, 3015, 3492, 3666, 4035
BfoI RGCGCY 2 cut(s) 74, 89
BfuAI ACCTGC 1 cut(s) 1111
BfuI GTATCC 2 cut(s) 1994, 2812
BglI GCCNNNNNGGC 1 cut(s) 4129
BlnI CCTAGG 1 cut(s) 4171
BmcAI AGTACT 4 cut(s) 1286, 2378, 3197, 3379
Bme1390I CCNGG 8 cut(s) 1177, 1178, 1765, 2113, 2568, 2898, 3234, 4042
Bme18I GGWCC 8 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170
BmeT110I CYCGRG 3 cut(s) 236, 1176, 1625
BmgBI CACGTC 1 cut(s) 122
BmiI GGNNCC 8 cut(s) 507, 932, 1173, 1174, 1680, 1927, 3172, 3601
BmrFI CCNGG 8 cut(s) 1177, 1178, 1765, 2113, 2568, 2898, 3234, 4042
BmrI ACTGGG 2 cut(s) 1017, 4033
BmsI GCATC 3 cut(s) 2995, 3246, 3519
BmtI GCTAGC 1 cut(s) 4103
BmuI ACTGGG 2 cut(s) 1017, 4033
BpiI GAAGAC 4 cut(s) 560, 1475, 1564, 4186
BpmI CTGGAG 6 cut(s) 94, 110, 197, 1092, 2574, 4063
Bpu10I CCTNAGC 3 cut(s) 183, 3365, 3735
BpuEI CTTGAG 1 cut(s) 1792
BpuMI CCSGG 4 cut(s) 1177, 1178, 2568, 2898
BsaBI GATNNNNATC 1 cut(s) 789
BsaHI GRCGYC 5 cut(s) 38, 150, 165, 553, 632
BsaI GGTCTC 3 cut(s) 1106, 2129, 3563
BsaWI WCCGGW 1 cut(s) 2138
BsaXI ACNNNNNCTCC 4 cut(s) 8, 38, 2340, 2370
Bsc4I CCNNNNNNNGG 7 cut(s) 518, 1744, 2876, 2996, 2997, 3295, 4008
Bse118I RCCGGY 1 cut(s) 2138
Bse8I GATNNNNATC 1 cut(s) 789
BseBI CCWGG 4 cut(s) 1765, 2113, 3234, 4042
BseGI GGATG 5 cut(s) 1000, 2683, 2989, 3261, 3711
BseJI GATNNNNATC 1 cut(s) 789
BseLI CCNNNNNNNGG 7 cut(s) 518, 1744, 2876, 2996, 2997, 3295, 4008
BseMII CTCAG 6 cut(s) 197, 398, 2360, 3431, 3497, 3749
BseRI GAGGAG 2 cut(s) 80, 2855
BseSI GKGCMC 3 cut(s) 1069, 1633, 2884
BseYI CCCAGC 2 cut(s) 3573, 3701
BsgI GTGCAG 3 cut(s) 1170, 1874, 3769
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 7 cut(s) 600, 933, 1155, 1694, 2566, 3232, 4056
BshNI GGYRCC 1 cut(s) 1678
BshTI ACCGGT 1 cut(s) 2138
BsiHKAI GWGCWC 5 cut(s) 243, 358, 518, 1069, 2865
BsiHKCI CYCGRG 3 cut(s) 236, 1176, 1625
BsiSI CCGG 8 cut(s) 519, 549, 1177, 1579, 2139, 2567, 2897, 4182
BslFI GGGAC 4 cut(s) 1185, 1206, 1736, 2474
BslI CCNNNNNNNGG 7 cut(s) 518, 1744, 2876, 2996, 2997, 3295, 4008
BsmBI CGTCTC 3 cut(s) 141, 897, 2014
BsmFI GGGAC 4 cut(s) 1185, 1206, 1736, 2474
BsmI GAATGC 2 cut(s) 1054, 1513
BsnI GGCC 7 cut(s) 600, 933, 1155, 1694, 2566, 3232, 4056
Bso31I GGTCTC 3 cut(s) 1106, 2129, 3563
BsoBI CYCGRG 3 cut(s) 236, 1176, 1625
Bsp1286I GDGCHC 8 cut(s) 243, 358, 518, 1014, 1069, 1633, 2865, 2884
Bsp19I CCATGG 3 cut(s) 601, 1644, 3594
BspANI GGCC 7 cut(s) 600, 933, 1155, 1694, 2566, 3232, 4056
BspCNI CTCAG 6 cut(s) 196, 399, 2361, 3430, 3496, 3748
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 3 cut(s) 804, 858, 3978
BspLI GGNNCC 8 cut(s) 507, 932, 1173, 1174, 1680, 1927, 3172, 3601
BspMAI CTGCAG 4 cut(s) 1381, 1423, 3019, 3496
BspMI ACCTGC 1 cut(s) 1111
BspOI GCTAGC 1 cut(s) 4103
BspPI GGATC 6 cut(s) 1835, 1920, 1933, 1972, 3594, 3607
BspQI GCTCTTC 1 cut(s) 2310
BspT107I GGYRCC 1 cut(s) 1678
BspTNI GGTCTC 3 cut(s) 1106, 2129, 3563
BsrFI RCCGGY 1 cut(s) 2138
BssAI RCCGGY 1 cut(s) 2138
BssNI GRCGYC 5 cut(s) 38, 150, 165, 553, 632
BssSI CACGAG 1 cut(s) 1062
BssT1I CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
Bst2BI CACGAG 1 cut(s) 1062
Bst2UI CCWGG 4 cut(s) 1765, 2113, 3234, 4042
Bst6I CTCTTC 6 cut(s) 751, 1445, 1761, 2310, 2319, 2517
BstACI GRCGYC 5 cut(s) 38, 150, 165, 553, 632
BstAPI GCANNNNNTGC 1 cut(s) 3014
BstC8I GCNNGC 7 cut(s) 173, 2173, 2729, 2798, 3371, 3771, 4101
BstDSI CCRYGG 4 cut(s) 601, 1644, 3594, 3801
BstF5I GGATG 5 cut(s) 1000, 2683, 2989, 3261, 3711
BstFNI CGCG 1 cut(s) 23
BstH2I RGCGCY 2 cut(s) 74, 89
BstHHI GCGC 4 cut(s) 73, 88, 887, 3769
BstNI CCWGG 4 cut(s) 1765, 2113, 3234, 4042
BstNSI RCATGY 1 cut(s) 3955
BstSCI CCNGG 8 cut(s) 1175, 1176, 1763, 2111, 2566, 2896, 3232, 4040
BstSFI CTRYAG 9 cut(s) 951, 1377, 1419, 2940, 2970, 3015, 3492, 3666, 4035
BstSLI GKGCMC 3 cut(s) 1069, 1633, 2884
BstUI CGCG 1 cut(s) 23
BstV2I GAAGAC 4 cut(s) 560, 1475, 1564, 4186
BstX2I RGATCY 3 cut(s) 1925, 1964, 3599
BstXI CCANNNNNNTGG 2 cut(s) 2034, 2620
BstYI RGATCY 3 cut(s) 1925, 1964, 3599
BsuI GTATCC 2 cut(s) 1994, 2812
BsuRI GGCC 7 cut(s) 600, 933, 1155, 1694, 2566, 3232, 4056
BtgI CCRYGG 4 cut(s) 601, 1644, 3594, 3801
BtrI CACGTC 1 cut(s) 122
BtsCI GGATG 5 cut(s) 1000, 2683, 2989, 3261, 3711
BtsI GCAGTG 4 cut(s) 356, 2952, 3489, 3642
BtsIMutI CAGTG 9 cut(s) 356, 1080, 1710, 2550, 2602, 2882, 2952, 3489, 3642
BveI ACCTGC 1 cut(s) 1111
Cac8I GCNNGC 7 cut(s) 173, 2173, 2729, 2798, 3371, 3771, 4101
CaiI CAGNNNCTG 1 cut(s) 190
CciI TCATGA 3 cut(s) 804, 858, 3978
CfoI GCGC 4 cut(s) 73, 88, 887, 3769
Cfr10I RCCGGY 1 cut(s) 2138
Cfr9I CCCGGG 1 cut(s) 1176
CseI GACGC 4 cut(s) 27, 158, 538, 561
CspAI ACCGGT 1 cut(s) 2138
CspCI CAANNNNNGTGG 4 cut(s) 448, 483, 1671, 1706
DraI TTTAAA 1 cut(s) 3828
EaeI YGGCCR 2 cut(s) 598, 1692
Eam1104I CTCTTC 6 cut(s) 751, 1445, 1761, 2310, 2319, 2517
EarI CTCTTC 6 cut(s) 751, 1445, 1761, 2310, 2319, 2517
EciI GGCGGA 3 cut(s) 29, 498, 683
Ecl136II GAGCTC 2 cut(s) 516, 2863
Eco130I CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
Eco147I AGGCCT 1 cut(s) 3232
Eco24I GRGCYC 3 cut(s) 518, 1014, 2865
Eco31I GGTCTC 3 cut(s) 1106, 2129, 3563
Eco47I GGWCC 8 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170
Eco53kI GAGCTC 2 cut(s) 516, 2863
Eco57I CTGAAG 5 cut(s) 212, 246, 554, 1487, 3300
Eco88I CYCGRG 3 cut(s) 236, 1176, 1625
EcoICRI GAGCTC 2 cut(s) 516, 2863
EcoO109I RGGNCCY 1 cut(s) 1172
EcoRI GAATTC 3 cut(s) 2096, 2514, 3996
EcoRII CCWGG 4 cut(s) 1763, 2111, 3232, 4040
EcoT14I CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
EcoT22I ATGCAT 1 cut(s) 1513
EcoT38I GRGCYC 3 cut(s) 518, 1014, 2865
ErhI CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
Esp3I CGTCTC 3 cut(s) 141, 897, 2014
FalI AAGNNNNNCTT 2 cut(s) 1460, 1492
FaqI GGGAC 4 cut(s) 1185, 1206, 1736, 2474
FauI CCCGC 3 cut(s) 763, 1232, 1852
FauNDI CATATG 1 cut(s) 881
FbaI TGATCA 1 cut(s) 1557
FokI GGATG 5 cut(s) 1007, 2670, 2996, 3268, 3718
FriOI GRGCYC 3 cut(s) 518, 1014, 2865
FspBI CTAG 9 cut(s) 740, 749, 965, 1854, 2013, 3090, 3533, 4100, 4172
FspI TGCGCA 1 cut(s) 3768
GlaI GCGC 4 cut(s) 72, 87, 886, 3768
GsaI CCCAGC 2 cut(s) 3577, 3705
GsuI CTGGAG 6 cut(s) 94, 110, 197, 1092, 2574, 4063
HaeII RGCGCY 2 cut(s) 74, 89
HaeIII GGCC 7 cut(s) 600, 933, 1155, 1694, 2566, 3232, 4056
HapII CCGG 8 cut(s) 519, 549, 1177, 1579, 2139, 2567, 2897, 4182
HgaI GACGC 4 cut(s) 27, 158, 538, 561
HhaI GCGC 4 cut(s) 73, 88, 887, 3769
Hin1I GRCGYC 5 cut(s) 38, 150, 165, 553, 632
Hin6I GCGC 4 cut(s) 71, 86, 885, 3767
HinP1I GCGC 4 cut(s) 71, 86, 885, 3767
HincII GTYRAC 5 cut(s) 163, 217, 650, 2812, 2916
HindII GTYRAC 5 cut(s) 163, 217, 650, 2812, 2916
HindIII AAGCTT 4 cut(s) 330, 1341, 1474, 1697
HpaI GTTAAC 2 cut(s) 217, 2812
HpaII CCGG 8 cut(s) 519, 549, 1177, 1579, 2139, 2567, 2897, 4182
HphI GGTGA 7 cut(s) 800, 968, 2857, 2914, 3625, 3868, 4088
Hpy99I CGWCG 5 cut(s) 43, 123, 532, 637, 2486
HpyAV CCTTC 9 cut(s) 492, 1264, 1359, 1450, 1770, 1895, 2654, 2821, 3206
Hsp92I GRCGYC 5 cut(s) 38, 150, 165, 553, 632
HspAI GCGC 4 cut(s) 71, 86, 885, 3767
KflI GGGWCCC 1 cut(s) 1172
Ksp22I TGATCA 1 cut(s) 1557
KspAI GTTAAC 2 cut(s) 217, 2812
LguI GCTCTTC 1 cut(s) 2310
LmnI GCTCC 9 cut(s) 187, 429, 521, 1111, 1459, 2555, 2860, 2950, 3946
LweI GCATC 3 cut(s) 2995, 3246, 3519
MaeI CTAG 9 cut(s) 740, 749, 965, 1854, 2013, 3090, 3533, 4100, 4172
MaeIII GTNAC 8 cut(s) 2394, 2548, 2720, 3265, 3631, 3717, 3904, 4076
MfeI CAATTG 2 cut(s) 1602, 3207
MflI RGATCY 3 cut(s) 1925, 1964, 3599
MhlI GDGCHC 8 cut(s) 243, 358, 518, 1014, 1069, 1633, 2865, 2884
MlsI TGGCCA 2 cut(s) 600, 1694
MluI ACGCGT 1 cut(s) 21
MluNI TGGCCA 2 cut(s) 600, 1694
MlyI GAGTC 7 cut(s) 123, 255, 682, 1805, 2601, 3639, 4054
MmeI TCCRAC 6 cut(s) 142, 700, 855, 2569, 3428, 3527
Mox20I TGGCCA 2 cut(s) 600, 1694
Mph1103I ATGCAT 1 cut(s) 1513
MroXI GAANNNNTTC 4 cut(s) 1344, 2772, 3435, 3965
MscI TGGCCA 2 cut(s) 600, 1694
MslI CAYNNNNRTG 3 cut(s) 2130, 2618, 3026
Msp20I TGGCCA 2 cut(s) 600, 1694
MspA1I CMGCKG 1 cut(s) 3972
MspI CCGG 8 cut(s) 519, 549, 1177, 1579, 2139, 2567, 2897, 4182
MspR9I CCNGG 8 cut(s) 1177, 1178, 1765, 2113, 2568, 2898, 3234, 4042
MunI CAATTG 2 cut(s) 1602, 3207
Mva1269I GAATGC 2 cut(s) 1054, 1513
MvaI CCWGG 4 cut(s) 1765, 2113, 3234, 4042
MvnI CGCG 1 cut(s) 23
NciI CCSGG 4 cut(s) 1177, 1178, 2568, 2898
NcoI CCATGG 3 cut(s) 601, 1644, 3594
NdeI CATATG 1 cut(s) 881
NheI GCTAGC 1 cut(s) 4099
NlaIV GGNNCC 8 cut(s) 507, 932, 1173, 1174, 1680, 1927, 3172, 3601
NmeAIII GCCGAG 4 cut(s) 200, 537, 3399, 3692
NmuCI GTSAC 5 cut(s) 2394, 2548, 3631, 3904, 4076
NsbI TGCGCA 1 cut(s) 3768
NsiI ATGCAT 1 cut(s) 1513
NspI RCATGY 1 cut(s) 3955
PagI TCATGA 3 cut(s) 804, 858, 3978
PceI AGGCCT 1 cut(s) 3232
PciSI GCTCTTC 1 cut(s) 2310
PctI GAATGC 2 cut(s) 1054, 1513
PdmI GAANNNNTTC 4 cut(s) 1344, 2772, 3435, 3965
PfeI GAWTC 9 cut(s) 723, 1073, 1181, 2383, 2768, 3107, 3552, 3961, 3982
PflFI GACNNNGTC 2 cut(s) 37, 708
PflMI CCANNNNNTGG 1 cut(s) 2876
PfoI TCCNGGA 1 cut(s) 2111
PinAI ACCGGT 1 cut(s) 2138
PleI GAGTC 7 cut(s) 122, 255, 682, 1804, 2600, 3638, 4053
PpsI GAGTC 7 cut(s) 122, 255, 682, 1804, 2600, 3638, 4053
PpuMI RGGWCCY 1 cut(s) 1172
PsiI TTATAA 1 cut(s) 476
Psp124BI GAGCTC 2 cut(s) 518, 2865
Psp1406I AACGTT 1 cut(s) 324
Psp5II RGGWCCY 1 cut(s) 1172
Psp6I CCWGG 4 cut(s) 1763, 2111, 3232, 4040
PspFI CCCAGC 2 cut(s) 3573, 3701
PspGI CCWGG 4 cut(s) 1763, 2111, 3232, 4040
PspN4I GGNNCC 8 cut(s) 507, 932, 1173, 1174, 1680, 1927, 3172, 3601
PspPPI RGGWCCY 1 cut(s) 1172
PstI CTGCAG 4 cut(s) 1381, 1423, 3019, 3496
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 3 cut(s) 1925, 1964, 3599
PsyI GACNNNGTC 2 cut(s) 37, 708
RseI CAYNNNNRTG 3 cut(s) 2130, 2618, 3026
SacI GAGCTC 2 cut(s) 518, 2865
SapI GCTCTTC 1 cut(s) 2310
ScaI AGTACT 4 cut(s) 1286, 2378, 3197, 3379
SchI GAGTC 7 cut(s) 123, 255, 682, 1805, 2601, 3639, 4054
ScrFI CCNGG 8 cut(s) 1177, 1178, 1765, 2113, 2568, 2898, 3234, 4042
SduI GDGCHC 8 cut(s) 243, 358, 518, 1014, 1069, 1633, 2865, 2884
SfaNI GCATC 3 cut(s) 2995, 3246, 3519
SfcI CTRYAG 9 cut(s) 951, 1377, 1419, 2940, 2970, 3015, 3492, 3666, 4035
SinI GGWCC 8 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170
SmaI CCCGGG 1 cut(s) 1178
SmiMI CAYNNNNRTG 3 cut(s) 2130, 2618, 3026
SmlI CTYRAG 1 cut(s) 1771
SmoI CTYRAG 1 cut(s) 1771
SpeI ACTAGT 1 cut(s) 964
SseBI AGGCCT 1 cut(s) 3232
SspMI CTAG 9 cut(s) 740, 749, 965, 1854, 2013, 3090, 3533, 4100, 4172
SstI GAGCTC 2 cut(s) 518, 2865
StuI AGGCCT 1 cut(s) 3232
StyD4I CCNGG 8 cut(s) 1175, 1176, 1763, 2111, 2566, 2896, 3232, 4040
StyI CCWWGG 9 cut(s) 153, 556, 601, 1644, 1929, 2101, 2283, 3594, 4171
TaqI TCGA 6 cut(s) 300, 1014, 1936, 2484, 2638, 3688
TaqII GACCGA 1 cut(s) 407
TatI WGTACW 7 cut(s) 852, 1284, 2376, 3195, 3377, 3486, 3708
TauI GCSGC 5 cut(s) 86, 625, 668, 946, 3975
TfiI GAWTC 9 cut(s) 723, 1073, 1181, 2383, 2768, 3107, 3552, 3961, 3982
TscAI CASTG 9 cut(s) 363, 1087, 1710, 2557, 2602, 2889, 2952, 3496, 3649
TseFI GTSAC 5 cut(s) 2394, 2548, 3631, 3904, 4076
Tsp45I GTSAC 5 cut(s) 2394, 2548, 3631, 3904, 4076
TspGWI ACGGA 5 cut(s) 1688, 2135, 2839, 3278, 3900
TspMI CCCGGG 1 cut(s) 1176
TspRI CASTG 9 cut(s) 363, 1087, 1710, 2557, 2602, 2889, 2952, 3496, 3649
Tth111I GACNNNGTC 2 cut(s) 37, 708
Van91I CCANNNNNTGG 1 cut(s) 2876
VneI GTGCAC 1 cut(s) 1065
VpaK11BI GGWCC 8 cut(s) 506, 971, 1172, 1273, 1290, 2431, 3161, 3170
XbaI TCTAGA 2 cut(s) 1853, 2012
XceI RCATGY 1 cut(s) 3955
XcmI CCANNNNNNNNNTGG 1 cut(s) 2193
XmaI CCCGGG 1 cut(s) 1176
XmaJI CCTAGG 1 cut(s) 4171
XmnI GAANNNNTTC 4 cut(s) 1344, 2772, 3435, 3965
XspI CTAG 9 cut(s) 740, 749, 965, 1854, 2013, 3090, 3533, 4100, 4172
ZraI GACGTC 2 cut(s) 166, 633
ZrmI AGTACT 4 cut(s) 1286, 2378, 3197, 3379
Zsp2I ATGCAT 1 cut(s) 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.