FvH4_1g04320

Nucleolar protein 12 (25kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011265
Physical Location & Seq
Forward (+)
2312537 .. 2315379
2843 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04320.t2

Sequence Viewer

Length: 615 bp
ATGGCAGCAGAGAAGGCTGAAGACGTGGCGATTCCGGCGGCAGCTCCGCGTGGCCGTTACGTCAACAAGAGGGCTCTTAAGAACAAGGCCGTCGCCGTCACCTTCAACGAGAAAAGTCTCAGTGACTTTGTGTCTGGGTTTCACAAGAGGAAGAAGAAGAGAAGAAAGGAGGCTGAGAAGAAGCAAGGGGAGGCTCTCAGGCGTAAGCTTCTTGAGCTCCGCAAAAAGAGGAGACTAGAAAAGGAATTGGCTCTCTTTGGTGGAGCTCCCCCTGCTGCTGAGGGAGAAGCTGATGAGAATGATGAGGGAGATGCGAGCCAGGAGGAAGATGAAGAAAGTGAGCCAGATGCACTGATTTCTGGTACAACAACTTATGACAATGGTGACATAAAAGTCACAGTGACAACAAGCGAGATCTCTCAAGAAGAGGAAATCCATCCAGTTAAGAAGACAGAGGCTGTAGTGCCAGAAACAGTTGGAGCTAATAAAAAGAACAATGTTCCTGTTACCAAAAAGAAACCTTTCAAAAAAGTTGGAAAACGCAAATCAGGGCCAAAGATGCAAAAGAAAAGAGATAAGAGGAAGGGAAAGGAAAAAGGCTCGAAGAGGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop25

Protein Analysis

205

Amino Acids

22.76

Weight (kDa)

9.86

Isoelectric Point (pI)

62.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop25 PF09805 23 - 147 2.7e-14 Nucleolar protein 12 (25kDa)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11240
fragaria_vesca FvH4_1g04320 FvH4_1g04320 FvH4_3g04070
malus_domestica MD05G1325000.v1.1 MD10G1302800.v1.1
prunus_persica Prupe.4G036300_v2.0.a1
pyrus_communis pycom05g29910 pycom10g25540
rosa_chinensis RchiOBHm_Chr5g0006551
rosa_laevigata RLG00000031398
rosa_multiflora Rmu_sc0008991.1_g000017
rosa_roxburghii Rroxscaffold_1G00069510
rosa_rugosa Rorug04G0422100 Rorug04G0435300
rosa_samantha Rh5AG049600 Rh5BG048900 Rh5CG057200 Rh5DG048200
rosa_wichuraiana Rw5G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AccII CGCG 1 cut(s) 49
AciI CCGC 3 cut(s) 38, 47, 220
AcoI YGGCCR 1 cut(s) 52
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 1 cut(s) 364
AflII CTTAAG 1 cut(s) 77
AgsI TTSAA 2 cut(s) 106, 526
AhdI GACNNNNNGTC 1 cut(s) 130
AjiI CACGTC 1 cut(s) 25
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 6 cut(s) 44, 208, 217, 266, 290, 482
AluI AGCT 6 cut(s) 44, 208, 217, 266, 290, 482
Alw21I GWGCWC 2 cut(s) 219, 268
Alw26I GTCTC 2 cut(s) 122, 226
AlwNI CAGNNNCTG 1 cut(s) 458
AoxI GGCC 3 cut(s) 52, 87, 551
ApeKI GCWGC 3 cut(s) 5, 41, 275
Asp700I GAANNNNTTC 1 cut(s) 521
AspLEI GCGC 1 cut(s) 612
AspS9I GGNCC 1 cut(s) 551
AsuHPI GGTGA 2 cut(s) 91, 395
BanII GRGCYC 3 cut(s) 76, 219, 268
BbsI GAAGAC 2 cut(s) 27, 455
Bbv12I GWGCWC 2 cut(s) 219, 268
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 3 cut(s) 17, 53, 262
BccI CCATC 1 cut(s) 444
BceAI ACGGC 3 cut(s) 39, 74, 80
BciT130I CCWGG 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 122, 226
BfaI CTAG 2 cut(s) 236, 613
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 613
BfrI CTTAAG 1 cut(s) 77
BglII AGATCT 1 cut(s) 414
BisI GCNGC 4 cut(s) 6, 39, 42, 276
BlsI GCNGC 4 cut(s) 7, 40, 43, 277
Bme1390I CCNGG 1 cut(s) 320
BmeRI GACNNNNNGTC 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 25
BmgT120I GGNCC 1 cut(s) 551
BmrFI CCNGG 1 cut(s) 320
BmsI GCATC 3 cut(s) 301, 337, 549
BpiI GAAGAC 2 cut(s) 27, 455
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 2 cut(s) 233, 405
Bse1I ACTGG 1 cut(s) 440
BseBI CCWGG 1 cut(s) 320
BseGI GGATG 1 cut(s) 436
BseMII CTCAG 4 cut(s) 133, 165, 211, 270
BseNI ACTGG 1 cut(s) 440
BseRI GAGGAG 1 cut(s) 244
BseXI GCAGC 3 cut(s) 17, 53, 262
Bsh1236I CGCG 1 cut(s) 49
BshFI GGCC 3 cut(s) 54, 89, 553
BsiHKAI GWGCWC 2 cut(s) 219, 268
BsiSI CCGG 1 cut(s) 35
BsmAI GTCTC 2 cut(s) 122, 226
BsnI GGCC 3 cut(s) 54, 89, 553
Bsp1286I GDGCHC 3 cut(s) 76, 219, 268
Bsp143I GATC 1 cut(s) 414
BspACI CCGC 3 cut(s) 38, 47, 220
BspANI GGCC 3 cut(s) 54, 89, 553
BspCNI CTCAG 4 cut(s) 132, 166, 210, 271
BspFNI CGCG 1 cut(s) 49
BspTI CTTAAG 1 cut(s) 77
BsrI ACTGG 1 cut(s) 440
BssMI GATC 1 cut(s) 414
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 2 cut(s) 400, 475
Bst6I CTCTTC 3 cut(s) 152, 420, 599
BstAFI CTTAAG 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 4 cut(s) 119, 174, 197, 279
BstF5I GGATG 1 cut(s) 436
BstFNI CGCG 1 cut(s) 49
BstH2I RGCGCY 1 cut(s) 613
BstHHI GCGC 1 cut(s) 612
BstKTI GATC 1 cut(s) 417
BstMAI GTCTC 2 cut(s) 122, 226
BstMBI GATC 1 cut(s) 414
BstMWI GCNNNNNNNGC 5 cut(s) 14, 35, 214, 272, 559
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 459
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 3 cut(s) 17, 53, 262
BstV2I GAAGAC 2 cut(s) 27, 455
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuRI GGCC 3 cut(s) 54, 89, 553
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 1 cut(s) 436
BtsIMutI CAGTG 3 cut(s) 127, 350, 405
Cac8I GCNNGC 1 cut(s) 316
CaiI CAGNNNCTG 1 cut(s) 458
CfoI GCGC 1 cut(s) 612
Cfr13I GGNCC 1 cut(s) 551
Csp6I GTAC 1 cut(s) 363
CviQI GTAC 1 cut(s) 363
DdeI CTNAG 4 cut(s) 119, 174, 197, 279
DpnI GATC 1 cut(s) 416
DpnII GATC 1 cut(s) 414
DrdI GACNNNNNNGTC 1 cut(s) 392
DriI GACNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 392
EaeI YGGCCR 1 cut(s) 52
Eam1104I CTCTTC 3 cut(s) 152, 420, 599
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 3 cut(s) 152, 420, 599
Ecl136II GAGCTC 2 cut(s) 217, 266
Eco24I GRGCYC 3 cut(s) 76, 219, 268
Eco53kI GAGCTC 2 cut(s) 217, 266
Eco57I CTGAAG 1 cut(s) 39
EcoICRI GAGCTC 2 cut(s) 217, 266
EcoRII CCWGG 1 cut(s) 318
EcoT38I GRGCYC 3 cut(s) 76, 219, 268
FaiI YATR 2 cut(s) 375, 389
Fnu4HI GCNGC 4 cut(s) 6, 39, 42, 276
FokI GGATG 1 cut(s) 423
FriOI GRGCYC 3 cut(s) 76, 219, 268
Fsp4HI GCNGC 4 cut(s) 6, 39, 42, 276
FspBI CTAG 2 cut(s) 236, 613
GlaI GCGC 1 cut(s) 611
GluI GCNGC 4 cut(s) 6, 39, 42, 276
HaeII RGCGCY 1 cut(s) 613
HaeIII GGCC 3 cut(s) 54, 89, 553
HapII CCGG 1 cut(s) 35
HhaI GCGC 1 cut(s) 612
Hin6I GCGC 1 cut(s) 610
HinP1I GCGC 1 cut(s) 610
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 1 cut(s) 31
HpaII CCGG 1 cut(s) 35
HphI GGTGA 2 cut(s) 91, 395
Hpy166II GTNNAC 1 cut(s) 64
Hpy188III TCNNGA 2 cut(s) 212, 422
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 3 cut(s) 7, 112, 577
HpyCH4III ACNGT 2 cut(s) 400, 475
HpyCH4IV ACGT 2 cut(s) 24, 60
HpyCH4V TGCA 2 cut(s) 350, 562
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 35, 214, 272, 559
HpyF3I CTNAG 4 cut(s) 119, 174, 197, 279
HpySE526I ACGT 2 cut(s) 24, 60
HspAI GCGC 1 cut(s) 610
Kzo9I GATC 1 cut(s) 414
LmnI GCTCC 5 cut(s) 49, 222, 263, 271, 479
Lsp1109I GCAGC 3 cut(s) 17, 53, 262
LweI GCATC 3 cut(s) 301, 337, 549
MaeI CTAG 2 cut(s) 236, 613
MaeII ACGT 2 cut(s) 24, 60
MaeIII GTNAC 7 cut(s) 56, 97, 122, 383, 394, 400, 505
MalI GATC 1 cut(s) 416
MboI GATC 1 cut(s) 414
MflI RGATCY 1 cut(s) 414
MhlI GDGCHC 3 cut(s) 76, 219, 268
MluCI AATT 1 cut(s) 245
MmeI TCCRAC 2 cut(s) 457, 514
MroXI GAANNNNTTC 1 cut(s) 521
MseI TTAA 2 cut(s) 78, 444
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 5 cut(s) 14, 35, 214, 272, 559
NdeII GATC 1 cut(s) 414
NmuCI GTSAC 5 cut(s) 97, 122, 383, 394, 400
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 4 cut(s) 7, 40, 43, 277
Psp124BI GAGCTC 2 cut(s) 219, 268
Psp6I CCWGG 1 cut(s) 318
PspGI CCWGG 1 cut(s) 318
PspPI GGNCC 1 cut(s) 551
PstNI CAGNNNCTG 1 cut(s) 458
PsuI RGATCY 1 cut(s) 414
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
SacI GAGCTC 2 cut(s) 219, 268
SaqAI TTAA 2 cut(s) 78, 444
SatI GCNGC 4 cut(s) 6, 39, 42, 276
Sau3AI GATC 1 cut(s) 414
Sau96I GGNCC 1 cut(s) 551
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 3 cut(s) 76, 219, 268
SfaNI GCATC 3 cut(s) 301, 337, 549
SfcI CTRYAG 1 cut(s) 459
SmlI CTYRAG 3 cut(s) 77, 212, 420
SmoI CTYRAG 3 cut(s) 77, 212, 420
Sse9I AATT 1 cut(s) 245
SsiI CCGC 3 cut(s) 38, 47, 220
SspMI CTAG 2 cut(s) 236, 613
SstI GAGCTC 2 cut(s) 219, 268
StyD4I CCNGG 1 cut(s) 318
TaaI ACNGT 2 cut(s) 400, 475
TaiI ACGT 2 cut(s) 27, 63
TaqI TCGA 1 cut(s) 602
TasI AATT 1 cut(s) 245
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 2 cut(s) 78, 444
Tru9I TTAA 2 cut(s) 78, 444
TscAI CASTG 3 cut(s) 127, 357, 405
TseFI GTSAC 5 cut(s) 97, 122, 383, 394, 400
TseI GCWGC 3 cut(s) 5, 41, 275
Tsp45I GTSAC 5 cut(s) 97, 122, 383, 394, 400
TspDTI ATGAA 1 cut(s) 345
TspRI CASTG 3 cut(s) 127, 357, 405
Vha464I CTTAAG 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 521
XspI CTAG 2 cut(s) 236, 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.