Rorug04G0435300

Nucleolar protein 12 (25kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0011265
Physical Location & Seq
Forward (+)
59296435 .. 59296824
390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0435300.1

Sequence Viewer

Length: 336 bp
ATGGGAAGATTTGAATTGGGTGGTCTTCCTGGCACATTATTGGAGCTGTTGTTTAAGGGCCATAGAGTTGGTCGCAACCCTCAACTAAAGGAGTTATGGATAGCTTGTTTTTGCTCAACCATGTGGTTCATTTGGCGAACAAGAAATATGTGCAAGTTTGAAGGTATTGTGCCCAATGCTATGCATGCTTGTAGAATGATCTCAGGTTATGTAAATACTTCCGGCCGCACAGCCACTGGCTGCATGTTCAACTCTATCCAGGATCTTTGTGTAGTTAAGCGATTTGGGGTTCCTTGTAAACCTCCTCGTGCACCCCGTATCATAGAGGTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop25

Protein Analysis

111

Amino Acids

12.58

Weight (kDa)

9.72

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11240
fragaria_vesca FvH4_1g04320 FvH4_1g04320 FvH4_3g04070
malus_domestica MD05G1325000.v1.1 MD10G1302800.v1.1
prunus_persica Prupe.4G036300_v2.0.a1
pyrus_communis pycom05g29910 pycom10g25540
rosa_chinensis RchiOBHm_Chr5g0006551
rosa_laevigata RLG00000031398
rosa_multiflora Rmu_sc0008991.1_g000017
rosa_roxburghii Rroxscaffold_1G00069510
rosa_rugosa Rorug04G0422100 Rorug04G0435300
rosa_samantha Rh5AG049600 Rh5BG048900 Rh5CG057200 Rh5DG048200
rosa_wichuraiana Rw5G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 226
AclWI GGATC 1 cut(s) 270
AcoI YGGCCR 1 cut(s) 223
AgsI TTSAA 3 cut(s) 14, 161, 250
AjnI CCWGG 2 cut(s) 28, 258
AluBI AGCT 2 cut(s) 46, 104
AluI AGCT 2 cut(s) 46, 104
Alw21I GWGCWC 1 cut(s) 313
Alw44I GTGCAC 1 cut(s) 309
AlwI GGATC 1 cut(s) 270
AlwNI CAGNNNCTG 1 cut(s) 236
AoxI GGCC 2 cut(s) 58, 223
ApaLI GTGCAC 1 cut(s) 309
ApeKI GCWGC 1 cut(s) 240
AspS9I GGNCC 1 cut(s) 58
BaeGI GKGCMC 2 cut(s) 174, 313
BauI CACGAG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 17
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 1 cut(s) 227
BciT130I CCWGG 2 cut(s) 30, 260
BisI GCNGC 2 cut(s) 226, 241
BlsI GCNGC 2 cut(s) 227, 242
Bme1390I CCNGG 2 cut(s) 30, 260
BmgT120I GGNCC 1 cut(s) 58
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 2 cut(s) 30, 260
BpiI GAAGAC 1 cut(s) 17
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bse1I ACTGG 1 cut(s) 241
BseBI CCWGG 2 cut(s) 30, 260
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 241
BseRI GAGGAG 1 cut(s) 294
BseSI GKGCMC 2 cut(s) 174, 313
BseX3I CGGCCG 1 cut(s) 223
BseXI GCAGC 1 cut(s) 227
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 2 cut(s) 60, 225
BsiEI CGRYCG 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 1 cut(s) 222
BsnI GGCC 2 cut(s) 60, 225
Bsp1286I GDGCHC 2 cut(s) 174, 313
Bsp143I GATC 2 cut(s) 198, 262
BspACI CCGC 1 cut(s) 226
BspANI GGCC 2 cut(s) 60, 225
BspCNI CTCAG 1 cut(s) 215
BspLI GGNNCC 1 cut(s) 291
BspPI GGATC 1 cut(s) 270
BsrI ACTGG 1 cut(s) 241
BssMI GATC 2 cut(s) 198, 262
BssSI CACGAG 1 cut(s) 306
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 30, 260
BstC8I GCNNGC 1 cut(s) 186
BstDEI CTNAG 1 cut(s) 202
BstKTI GATC 2 cut(s) 201, 265
BstMBI GATC 2 cut(s) 198, 262
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 2 cut(s) 30, 260
BstNSI RCATGY 2 cut(s) 188, 247
BstSCI CCNGG 2 cut(s) 28, 258
BstSLI GKGCMC 2 cut(s) 174, 313
BstV1I GCAGC 1 cut(s) 227
BstV2I GAAGAC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 262
BstXI CCANNNNNNTGG 1 cut(s) 68
BstYI RGATCY 1 cut(s) 262
BstZI CGGCCG 1 cut(s) 223
BsuRI GGCC 2 cut(s) 60, 225
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 1 cut(s) 186
CaiI CAGNNNCTG 1 cut(s) 236
Cfr13I GGNCC 1 cut(s) 58
CviAII CATG 3 cut(s) 121, 185, 244
CviJI RGCY 6 cut(s) 46, 60, 104, 225, 233, 240
CviKI_1 RGCY 6 cut(s) 46, 60, 104, 225, 233, 240
DdeI CTNAG 1 cut(s) 202
DpnI GATC 2 cut(s) 200, 264
DpnII GATC 2 cut(s) 198, 262
EaeI YGGCCR 1 cut(s) 223
EagI CGGCCG 1 cut(s) 223
EclXI CGGCCG 1 cut(s) 223
Eco52I CGGCCG 1 cut(s) 223
EcoRII CCWGG 2 cut(s) 28, 258
EcoT22I ATGCAT 1 cut(s) 186
FaeI CATG 3 cut(s) 124, 188, 247
FaiI YATR 9 cut(s) 63, 97, 122, 149, 182, 186, 210, 245, 323
FatI CATG 3 cut(s) 120, 184, 243
Fnu4HI GCNGC 2 cut(s) 226, 241
Fsp4HI GCNGC 2 cut(s) 226, 241
GluI GCNGC 2 cut(s) 226, 241
HaeIII GGCC 2 cut(s) 60, 225
HapII CCGG 1 cut(s) 222
Hin1II CATG 3 cut(s) 124, 188, 247
HpaII CCGG 1 cut(s) 222
Hpy166II GTNNAC 2 cut(s) 299, 311
Hpy8I GTNNAC 2 cut(s) 299, 311
HpyAV CCTTC 1 cut(s) 155
HpyCH4V TGCA 4 cut(s) 153, 184, 243, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 1 cut(s) 202
Hsp92II CATG 3 cut(s) 124, 188, 247
Kzo9I GATC 2 cut(s) 198, 262
LmnI GCTCC 1 cut(s) 43
LpnPI CCDG 7 cut(s) 15, 42, 189, 222, 235, 245, 272
Lsp1109I GCAGC 1 cut(s) 227
MalI GATC 2 cut(s) 200, 264
MboI GATC 2 cut(s) 198, 262
MboII GAAGA 2 cut(s) 17, 18
MflI RGATCY 1 cut(s) 262
MhlI GDGCHC 2 cut(s) 174, 313
MluCI AATT 2 cut(s) 14, 331
MnlI CCTC 4 cut(s) 90, 312, 315, 319
Mph1103I ATGCAT 1 cut(s) 186
MseI TTAA 2 cut(s) 54, 276
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 30, 260
MvaI CCWGG 2 cut(s) 30, 260
MwoI GCNNNNNNNGC 1 cut(s) 185
NdeII GATC 2 cut(s) 198, 262
NlaIII CATG 3 cut(s) 124, 188, 247
NlaIV GGNNCC 1 cut(s) 291
NsiI ATGCAT 1 cut(s) 186
NspI RCATGY 2 cut(s) 188, 247
PaeI GCATGC 1 cut(s) 188
PcsI WCGNNNNNNNCGW 1 cut(s) 313
PfoI TCCNGGA 1 cut(s) 258
PkrI GCNGC 2 cut(s) 227, 242
Psp6I CCWGG 2 cut(s) 28, 258
PspGI CCWGG 2 cut(s) 28, 258
PspN4I GGNNCC 1 cut(s) 291
PspPI GGNCC 1 cut(s) 58
PstNI CAGNNNCTG 1 cut(s) 236
PsuI RGATCY 1 cut(s) 262
SaqAI TTAA 2 cut(s) 54, 276
SatI GCNGC 2 cut(s) 226, 241
Sau3AI GATC 2 cut(s) 198, 262
Sau96I GGNCC 1 cut(s) 58
ScrFI CCNGG 2 cut(s) 30, 260
SduI GDGCHC 2 cut(s) 174, 313
SetI ASST 6 cut(s) 48, 106, 166, 208, 304, 330
SphI GCATGC 1 cut(s) 188
Sse9I AATT 2 cut(s) 14, 331
SsiI CCGC 1 cut(s) 226
StyD4I CCNGG 2 cut(s) 28, 258
TasI AATT 2 cut(s) 14, 331
TauI GCSGC 1 cut(s) 228
Tru1I TTAA 2 cut(s) 54, 276
Tru9I TTAA 2 cut(s) 54, 276
TscAI CASTG 1 cut(s) 241
TseI GCWGC 1 cut(s) 240
TspDTI ATGAA 1 cut(s) 118
TspRI CASTG 1 cut(s) 241
VneI GTGCAC 1 cut(s) 309
XceI RCATGY 2 cut(s) 188, 247
Zsp2I ATGCAT 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.