RchiOBHm_Chr5g0006551

Nucleolar protein 12 (25kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0011265
Physical Location & Seq
Reverse (-)
4043962 .. 4046191
2230 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28765

Sequence Viewer

Length: 615 bp
ATGGCAGCAGACAAGGCTGAAGACTTGGCGATTCCGGCCGCCGCTCCCCGCGGCCGTTACATCAACAAGAGGGCTCTTAAAAACAAGGCCGTCGCCGTCACTTTCAACGAGAAAGATCTCAGCGACTTTGTTTCTGGGTTTCACAAGAGGAAGAAGAAGAGGAGAAAGGAAGCTGAGAAGAAGCAAGGGGAGGCTTTAAGGCGGAAGCTTCTTGAGCTCCGCAAAAAGAGGAGACTAGAAAAGGAATTGGCTCTCTTTGGTGGAGCTCCCCCAGCTGCTGAGGGAGAAGCTGACGAGAATGATGAGGGAGATGTGAGCCAGGAGGAAGATGAAGAAAGTGAGCCAGATGCACCAATTTCTGGTACAACTACTTACGACAATGGAGACATAAAAGTCACAGTGACGACAAGCGAGATCTCTCAGGAAGAGGAAATACATCCAGTTAGAAAGACAGAGGCTGTAGTGCCCGAAACAGCTGGAGCTAATAAAAAGAACAGTGTTCCTGTGATAAAAAAGAAACCATTTAAAAAAGTTGGAAAGCGCAAATCAGGGCCAAAGATGCAAAAGAAAAGAGATAAAAGGAAAGGAAAGGAAAATAACTCGAAGAGGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop25

Protein Analysis

204

Amino Acids

22.85

Weight (kDa)

9.8

Isoelectric Point (pI)

63.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop25 PF09805 23 - 152 5.8e-14 Nucleolar protein 12 (25kDa)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11240
fragaria_vesca FvH4_1g04320 FvH4_1g04320 FvH4_3g04070
malus_domestica MD05G1325000.v1.1 MD10G1302800.v1.1
prunus_persica Prupe.4G036300_v2.0.a1
pyrus_communis pycom05g29910 pycom10g25540
rosa_chinensis RchiOBHm_Chr5g0006551
rosa_laevigata RLG00000031398
rosa_multiflora Rmu_sc0008991.1_g000017
rosa_roxburghii Rroxscaffold_1G00069510
rosa_rugosa Rorug04G0422100 Rorug04G0435300
rosa_samantha Rh5AG049600 Rh5BG048900 Rh5CG057200 Rh5DG048200
rosa_wichuraiana Rw5G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 392
AccB7I CCANNNNNTGG 1 cut(s) 359
AccBSI CCGCTC 1 cut(s) 44
AccII CGCG 1 cut(s) 51
AciI CCGC 6 cut(s) 39, 42, 49, 51, 202, 220
AcoI YGGCCR 2 cut(s) 36, 52
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 1 cut(s) 364
AfiI CCNNNNNNNGG 1 cut(s) 359
AgsI TTSAA 1 cut(s) 106
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 8 cut(s) 173, 208, 217, 266, 275, 290, 476, 482
AluI AGCT 8 cut(s) 173, 208, 217, 266, 275, 290, 476, 482
Alw21I GWGCWC 2 cut(s) 219, 268
Alw26I GTCTC 2 cut(s) 226, 378
AlwNI CAGNNNCTG 2 cut(s) 278, 458
AoxI GGCC 4 cut(s) 36, 52, 87, 551
ApeKI GCWGC 2 cut(s) 5, 275
AspLEI GCGC 2 cut(s) 543, 612
AspS9I GGNCC 1 cut(s) 551
BaeGI GKGCMC 1 cut(s) 468
BanII GRGCYC 3 cut(s) 76, 219, 268
BarI GAAGNNNNNNTAC 2 cut(s) 417, 449
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 2 cut(s) 219, 268
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 17, 262
BceAI ACGGC 3 cut(s) 39, 74, 80
BciT130I CCWGG 1 cut(s) 320
BcoDI GTCTC 2 cut(s) 226, 378
BfaI CTAG 2 cut(s) 236, 613
BfmI CTRYAG 1 cut(s) 459
BfoI RGCGCY 1 cut(s) 613
BglII AGATCT 2 cut(s) 115, 414
BisI GCNGC 5 cut(s) 6, 39, 42, 52, 276
BlsI GCNGC 5 cut(s) 7, 40, 43, 53, 277
Bme1390I CCNGG 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 551
BmrFI CCNGG 1 cut(s) 320
BmsI GCATC 2 cut(s) 337, 549
BpiI GAAGAC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 498
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 233
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 1 cut(s) 359
Bse1I ACTGG 1 cut(s) 440
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 436
BseLI CCNNNNNNNGG 1 cut(s) 359
BseMII CTCAG 4 cut(s) 133, 165, 270, 434
BseNI ACTGG 1 cut(s) 440
BseRI GAGGAG 2 cut(s) 175, 244
BseSI GKGCMC 1 cut(s) 468
BseX3I CGGCCG 2 cut(s) 36, 52
BseXI GCAGC 2 cut(s) 17, 262
BseYI CCCAGC 1 cut(s) 271
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 2 cut(s) 39, 55
BshFI GGCC 4 cut(s) 38, 54, 89, 553
BsiEI CGRYCG 2 cut(s) 39, 55
BsiHKAI GWGCWC 2 cut(s) 219, 268
BsiSI CCGG 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 359
BsmAI GTCTC 2 cut(s) 226, 378
BsnI GGCC 4 cut(s) 38, 54, 89, 553
Bsp1286I GDGCHC 4 cut(s) 76, 219, 268, 468
Bsp143I GATC 2 cut(s) 115, 414
BspACI CCGC 6 cut(s) 39, 42, 49, 51, 202, 220
BspANI GGCC 4 cut(s) 38, 54, 89, 553
BspCNI CTCAG 4 cut(s) 132, 166, 271, 433
BspFNI CGCG 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 44
BsrI ACTGG 1 cut(s) 440
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 2 cut(s) 115, 414
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 2 cut(s) 400, 497
Bst6I CTCTTC 3 cut(s) 152, 420, 599
BstDEI CTNAG 4 cut(s) 119, 174, 279, 420
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 436
BstFNI CGCG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 613
BstHHI GCGC 2 cut(s) 543, 612
BstKTI GATC 2 cut(s) 118, 417
BstMAI GTCTC 2 cut(s) 226, 378
BstMBI GATC 2 cut(s) 115, 414
BstMCI CGRYCG 2 cut(s) 39, 55
BstMWI GCNNNNNNNGC 5 cut(s) 14, 35, 214, 272, 559
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 318
BstSFI CTRYAG 1 cut(s) 459
BstSLI GKGCMC 1 cut(s) 468
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 17, 262
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 2 cut(s) 115, 414
BstYI RGATCY 2 cut(s) 115, 414
BstZI CGGCCG 2 cut(s) 36, 52
BsuRI GGCC 4 cut(s) 38, 54, 89, 553
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 436
BtsIMutI CAGTG 2 cut(s) 405, 502
CaiI CAGNNNCTG 2 cut(s) 278, 458
CfoI GCGC 2 cut(s) 543, 612
Cfr13I GGNCC 1 cut(s) 551
Cfr42I CCGCGG 1 cut(s) 52
Csp6I GTAC 1 cut(s) 363
CviQI GTAC 1 cut(s) 363
DdeI CTNAG 4 cut(s) 119, 174, 279, 420
DpnI GATC 2 cut(s) 117, 416
DpnII GATC 2 cut(s) 115, 414
DraI TTTAAA 1 cut(s) 526
DrdI GACNNNNNNGTC 1 cut(s) 392
DseDI GACNNNNNNGTC 1 cut(s) 392
EaeI YGGCCR 2 cut(s) 36, 52
EagI CGGCCG 2 cut(s) 36, 52
Eam1104I CTCTTC 3 cut(s) 152, 420, 599
EarI CTCTTC 3 cut(s) 152, 420, 599
EciI GGCGGA 1 cut(s) 217
Ecl136II GAGCTC 2 cut(s) 217, 266
EclXI CGGCCG 2 cut(s) 36, 52
Eco24I GRGCYC 3 cut(s) 76, 219, 268
Eco52I CGGCCG 2 cut(s) 36, 52
Eco53kI GAGCTC 2 cut(s) 217, 266
Eco57I CTGAAG 1 cut(s) 39
EcoICRI GAGCTC 2 cut(s) 217, 266
EcoRII CCWGG 1 cut(s) 318
EcoT38I GRGCYC 3 cut(s) 76, 219, 268
FaiI YATR 1 cut(s) 389
FauI CCCGC 1 cut(s) 56
Fnu4HI GCNGC 5 cut(s) 6, 39, 42, 52, 276
FokI GGATG 1 cut(s) 423
FriOI GRGCYC 3 cut(s) 76, 219, 268
Fsp4HI GCNGC 5 cut(s) 6, 39, 42, 52, 276
FspBI CTAG 2 cut(s) 236, 613
GlaI GCGC 2 cut(s) 542, 611
GluI GCNGC 5 cut(s) 6, 39, 42, 52, 276
GsaI CCCAGC 1 cut(s) 275
GsuI CTGGAG 1 cut(s) 498
HaeII RGCGCY 1 cut(s) 613
HaeIII GGCC 4 cut(s) 38, 54, 89, 553
HapII CCGG 1 cut(s) 35
HhaI GCGC 2 cut(s) 543, 612
Hin6I GCGC 2 cut(s) 541, 610
HinP1I GCGC 2 cut(s) 541, 610
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 1 cut(s) 31
HpaII CCGG 1 cut(s) 35
Hpy188III TCNNGA 2 cut(s) 212, 422
Hpy99I CGWCG 1 cut(s) 95
HpyCH4III ACNGT 2 cut(s) 400, 497
HpyCH4V TGCA 2 cut(s) 350, 562
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 35, 214, 272, 559
HpyF3I CTNAG 4 cut(s) 119, 174, 279, 420
HspAI GCGC 2 cut(s) 541, 610
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 2 cut(s) 115, 414
LmnI GCTCC 5 cut(s) 49, 222, 263, 271, 479
Lsp1109I GCAGC 2 cut(s) 17, 262
LweI GCATC 2 cut(s) 337, 549
MaeI CTAG 2 cut(s) 236, 613
MaeIII GTNAC 4 cut(s) 56, 97, 394, 400
MalI GATC 2 cut(s) 117, 416
MbiI CCGCTC 1 cut(s) 44
MboI GATC 2 cut(s) 115, 414
MboII GAAGA 8 cut(s) 32, 163, 166, 169, 190, 338, 344, 437
MflI RGATCY 2 cut(s) 115, 414
MhlI GDGCHC 4 cut(s) 76, 219, 268, 468
MluCI AATT 2 cut(s) 245, 354
MmeI TCCRAC 1 cut(s) 514
MseI TTAA 3 cut(s) 78, 197, 525
MspA1I CMGCKG 3 cut(s) 51, 275, 476
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 320
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 5 cut(s) 14, 35, 214, 272, 559
NdeII GATC 2 cut(s) 115, 414
NmuCI GTSAC 3 cut(s) 97, 394, 400
PfeI GAWTC 1 cut(s) 31
PflMI CCANNNNNTGG 1 cut(s) 359
PkrI GCNGC 5 cut(s) 7, 40, 43, 53, 277
Psp124BI GAGCTC 2 cut(s) 219, 268
Psp6I CCWGG 1 cut(s) 318
PspFI CCCAGC 1 cut(s) 271
PspGI CCWGG 1 cut(s) 318
PspPI GGNCC 1 cut(s) 551
PstNI CAGNNNCTG 2 cut(s) 278, 458
PsuI RGATCY 2 cut(s) 115, 414
PvuII CAGCTG 2 cut(s) 275, 476
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
SacI GAGCTC 2 cut(s) 219, 268
SacII CCGCGG 1 cut(s) 52
SaqAI TTAA 3 cut(s) 78, 197, 525
SatI GCNGC 5 cut(s) 6, 39, 42, 52, 276
Sau3AI GATC 2 cut(s) 115, 414
Sau96I GGNCC 1 cut(s) 551
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 4 cut(s) 76, 219, 268, 468
SetI ASST 8 cut(s) 175, 210, 219, 268, 277, 292, 478, 484
SfaNI GCATC 2 cut(s) 337, 549
SfcI CTRYAG 1 cut(s) 459
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 212
SmoI CTYRAG 1 cut(s) 212
Sse9I AATT 2 cut(s) 245, 354
SsiI CCGC 6 cut(s) 39, 42, 49, 51, 202, 220
SspMI CTAG 2 cut(s) 236, 613
SstI GAGCTC 2 cut(s) 219, 268
StyD4I CCNGG 1 cut(s) 318
TaaI ACNGT 2 cut(s) 400, 497
TaqI TCGA 1 cut(s) 602
TasI AATT 2 cut(s) 245, 354
TauI GCSGC 3 cut(s) 41, 44, 54
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 3 cut(s) 78, 197, 525
Tru9I TTAA 3 cut(s) 78, 197, 525
TscAI CASTG 2 cut(s) 405, 502
TseFI GTSAC 3 cut(s) 97, 394, 400
TseI GCWGC 2 cut(s) 5, 275
Tsp45I GTSAC 3 cut(s) 97, 394, 400
TspDTI ATGAA 1 cut(s) 345
TspRI CASTG 2 cut(s) 405, 502
Van91I CCANNNNNTGG 1 cut(s) 359
XspI CTAG 2 cut(s) 236, 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.