Prupe.4G036300_v2.0.a1

Nucleolar protein 12 (25kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0011265
Physical Location & Seq
Forward (+)
1719211 .. 1722037
2827 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G036300.1

Sequence Viewer

Length: 603 bp
ATGGTAGGCAAGGTCGAAGTGGAAGCGATTCCGGCGCCAAATCCCCGAGGCCGTCACATAAAGAAGAGGGCTCTTAAAAACAAGGCTCTCGCCGTCACGTTCAACGAGAAAGATCTCAGCGATTTTGTGTCTGGGTTTCACAAGAGGAAGAAGAAAAGGAGAAAGGAAGCCGAGAAGAAGCAAGAGGAGGCGCTACGGCGTAAGCGTCTTGAGCTGCGCAAAAAGAGGAGACTAGAAAAGGAACTGGCTCTTAATGATGACGCTCCCCCAGCCACTGACACAGCAGCTGATGAGAGTGATGAGCATGACGAGGATGATGAAAGTAGTGAGCCAGCTCTACCAGTTTCAGGTACAACGACTTATGACAATGGTGACATGAAAGTCACTGTGACAACAAGCGAGATCTCTCGAGAAGAGGAAAGTGATTCAGATGAAAAGACAGAGGCAGCAATACCCCAATCAGTTGGAGCTTCTAAAAAGCACAATGTGCCTGTGAGCAAGACAAAACCTTTGAAAAAAGTTGCAAAACGAAAATCACGGCCCAAGCTGCAACGTAAAAGAGATAAAAGGAAGGGACCGGAAAAGACCAAGAAGAGGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop25

Protein Analysis

201

Amino Acids

22.76

Weight (kDa)

9.92

Isoelectric Point (pI)

70.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11240
fragaria_vesca FvH4_1g04320 FvH4_1g04320 FvH4_3g04070
malus_domestica MD05G1325000.v1.1 MD10G1302800.v1.1
prunus_persica Prupe.4G036300_v2.0.a1
pyrus_communis pycom05g29910 pycom10g25540
rosa_chinensis RchiOBHm_Chr5g0006551
rosa_laevigata RLG00000031398
rosa_multiflora Rmu_sc0008991.1_g000017
rosa_roxburghii Rroxscaffold_1G00069510
rosa_rugosa Rorug04G0422100 Rorug04G0435300
rosa_samantha Rh5AG049600 Rh5BG048900 Rh5CG057200 Rh5DG048200
rosa_wichuraiana Rw5G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 380
Acc16I TGCGCA 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 34
AcyI GRCGYC 1 cut(s) 35
AdeI CACNNNGTG 1 cut(s) 487
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 2 cut(s) 347, 594
AgsI TTSAA 2 cut(s) 103, 514
AluBI AGCT 5 cut(s) 214, 287, 335, 470, 547
AluI AGCT 5 cut(s) 214, 287, 335, 470, 547
Alw26I GTCTC 1 cut(s) 223
AlwNI CAGNNNCTG 2 cut(s) 275, 287
Ama87I CYCGRG 2 cut(s) 45, 408
AoxI GGCC 2 cut(s) 49, 539
ApeKI GCWGC 4 cut(s) 214, 284, 446, 547
ArsI GACNNNNNNTTYG 2 cut(s) 493, 525
Asp700I GAANNNNTTC 1 cut(s) 27
AspLEI GCGC 4 cut(s) 37, 193, 219, 600
AspS9I GGNCC 2 cut(s) 540, 575
AsuHPI GGTGA 1 cut(s) 383
AvaI CYCGRG 2 cut(s) 45, 408
AvaII GGWCC 1 cut(s) 575
BanI GGYRCC 1 cut(s) 34
BanII GRGCYC 1 cut(s) 73
BbvI GCAGC 4 cut(s) 201, 296, 458, 534
BceAI ACGGC 4 cut(s) 36, 77, 212, 554
BcoDI GTCTC 1 cut(s) 223
BfaI CTAG 2 cut(s) 233, 601
BfoI RGCGCY 3 cut(s) 38, 194, 601
BglII AGATCT 2 cut(s) 112, 402
BisI GCNGC 4 cut(s) 215, 285, 447, 548
BlsI GCNGC 4 cut(s) 216, 286, 448, 549
Bme18I GGWCC 1 cut(s) 575
BmeT110I CYCGRG 2 cut(s) 45, 408
BmgT120I GGNCC 2 cut(s) 540, 575
BmiI GGNNCC 2 cut(s) 36, 576
BpuEI CTTGAG 1 cut(s) 230
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 1 cut(s) 46
BsaWI WCCGGW 1 cut(s) 577
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 594
Bse1I ACTGG 2 cut(s) 249, 341
BseDI CCNNGG 1 cut(s) 46
BseGI GGATG 1 cut(s) 319
BseLI CCNNNNNNNGG 2 cut(s) 347, 594
BseMII CTCAG 1 cut(s) 130
BseNI ACTGG 2 cut(s) 249, 341
BseRI GAGGAG 2 cut(s) 200, 241
BseXI GCAGC 4 cut(s) 201, 296, 458, 534
BseYI CCCAGC 1 cut(s) 268
BshFI GGCC 2 cut(s) 51, 541
BshNI GGYRCC 1 cut(s) 34
BsiHKCI CYCGRG 2 cut(s) 45, 408
BsiSI CCGG 2 cut(s) 32, 578
BslFI GGGAC 1 cut(s) 588
BslI CCNNNNNNNGG 2 cut(s) 347, 594
BsmAI GTCTC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 588
BsnI GGCC 2 cut(s) 51, 541
BsoBI CYCGRG 2 cut(s) 45, 408
Bsp1286I GDGCHC 1 cut(s) 73
Bsp143I GATC 2 cut(s) 112, 402
BspANI GGCC 2 cut(s) 51, 541
BspCNI CTCAG 1 cut(s) 129
BspLI GGNNCC 2 cut(s) 36, 576
BspT107I GGYRCC 1 cut(s) 34
BsrI ACTGG 2 cut(s) 249, 341
BssECI CCNNGG 1 cut(s) 46
BssMI GATC 2 cut(s) 112, 402
BssNI GRCGYC 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 3 cut(s) 59, 408, 587
BstACI GRCGYC 1 cut(s) 35
BstAPI GCANNNNNTGC 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 1 cut(s) 116
BstF5I GGATG 1 cut(s) 319
BstH2I RGCGCY 3 cut(s) 38, 194, 601
BstHHI GCGC 4 cut(s) 37, 193, 219, 600
BstKTI GATC 2 cut(s) 115, 405
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 2 cut(s) 112, 402
BstMWI GCNNNNNNNGC 5 cut(s) 32, 211, 269, 487, 547
BstV1I GCAGC 4 cut(s) 201, 296, 458, 534
BstX2I RGATCY 2 cut(s) 112, 402
BstXI CCANNNNNNTGG 1 cut(s) 464
BstYI RGATCY 2 cut(s) 112, 402
BsuRI GGCC 2 cut(s) 51, 541
BtsCI GGATG 1 cut(s) 319
BtsIMutI CAGTG 2 cut(s) 273, 384
Cac8I GCNNGC 1 cut(s) 333
CaiI CAGNNNCTG 2 cut(s) 275, 287
CfoI GCGC 4 cut(s) 37, 193, 219, 600
Cfr13I GGNCC 2 cut(s) 540, 575
CseI GACGC 2 cut(s) 194, 269
Csp6I GTAC 1 cut(s) 351
CviAII CATG 2 cut(s) 305, 376
CviQI GTAC 1 cut(s) 351
DdeI CTNAG 1 cut(s) 116
DinI GGCGCC 1 cut(s) 36
DpnI GATC 2 cut(s) 114, 404
DpnII GATC 2 cut(s) 112, 402
DraIII CACNNNGTG 1 cut(s) 487
DrdI GACNNNNNNGTC 1 cut(s) 380
DseDI GACNNNNNNGTC 1 cut(s) 380
Eam1104I CTCTTC 3 cut(s) 59, 408, 587
EarI CTCTTC 3 cut(s) 59, 408, 587
Eco24I GRGCYC 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 575
Eco88I CYCGRG 2 cut(s) 45, 408
EcoT38I GRGCYC 1 cut(s) 73
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
FaeI CATG 2 cut(s) 308, 379
FaiI YATR 4 cut(s) 59, 306, 363, 377
FaqI GGGAC 1 cut(s) 588
FatI CATG 2 cut(s) 304, 375
Fnu4HI GCNGC 4 cut(s) 215, 285, 447, 548
FokI GGATG 1 cut(s) 326
FriOI GRGCYC 1 cut(s) 73
Fsp4HI GCNGC 4 cut(s) 215, 285, 447, 548
FspBI CTAG 2 cut(s) 233, 601
FspI TGCGCA 1 cut(s) 218
GlaI GCGC 4 cut(s) 36, 192, 218, 599
GluI GCNGC 4 cut(s) 215, 285, 447, 548
GsaI CCCAGC 1 cut(s) 272
HaeII RGCGCY 3 cut(s) 38, 194, 601
HaeIII GGCC 2 cut(s) 51, 541
HapII CCGG 2 cut(s) 32, 578
HgaI GACGC 2 cut(s) 194, 269
HhaI GCGC 4 cut(s) 37, 193, 219, 600
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 2 cut(s) 308, 379
Hin6I GCGC 4 cut(s) 35, 191, 217, 598
HinP1I GCGC 4 cut(s) 35, 191, 217, 598
HinfI GANTC 2 cut(s) 28, 425
HpaII CCGG 2 cut(s) 32, 578
HphI GGTGA 1 cut(s) 383
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 3 cut(s) 209, 408, 410
HpyAV CCTTC 1 cut(s) 565
HpyCH4III ACNGT 1 cut(s) 388
HpyCH4IV ACGT 2 cut(s) 98, 553
HpyCH4V TGCA 2 cut(s) 524, 550
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 211, 269, 487, 547
HpyF3I CTNAG 1 cut(s) 116
HpySE526I ACGT 2 cut(s) 98, 553
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 2 cut(s) 308, 379
HspAI GCGC 4 cut(s) 35, 191, 217, 598
KasI GGCGCC 1 cut(s) 34
Kzo9I GATC 2 cut(s) 112, 402
LmnI GCTCC 2 cut(s) 268, 467
LpnPI CCDG 9 cut(s) 45, 117, 230, 282, 333, 345, 354, 504, 591
Lsp1109I GCAGC 4 cut(s) 201, 296, 458, 534
MaeI CTAG 2 cut(s) 233, 601
MaeII ACGT 2 cut(s) 98, 553
MaeIII GTNAC 5 cut(s) 53, 94, 371, 382, 388
MalI GATC 2 cut(s) 114, 404
MboI GATC 2 cut(s) 112, 402
MboII GAAGA 5 cut(s) 76, 160, 163, 187, 425
MflI RGATCY 2 cut(s) 112, 402
MhlI GDGCHC 1 cut(s) 73
Mly113I GGCGCC 1 cut(s) 35
MmeI TCCRAC 1 cut(s) 445
MroXI GAANNNNTTC 1 cut(s) 27
MseI TTAA 2 cut(s) 75, 252
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 2 cut(s) 32, 578
MwoI GCNNNNNNNGC 5 cut(s) 32, 211, 269, 487, 547
NarI GGCGCC 1 cut(s) 35
NdeII GATC 2 cut(s) 112, 402
NlaIII CATG 2 cut(s) 308, 379
NlaIV GGNNCC 2 cut(s) 36, 576
NmeAIII GCCGAG 1 cut(s) 196
NmuCI GTSAC 5 cut(s) 53, 94, 371, 382, 388
NsbI TGCGCA 1 cut(s) 218
PaeR7I CTCGAG 1 cut(s) 408
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 2 cut(s) 28, 425
PkrI GCNGC 4 cut(s) 216, 286, 448, 549
PluTI GGCGCC 1 cut(s) 38
PspFI CCCAGC 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 36, 576
PspPI GGNCC 2 cut(s) 540, 575
PstNI CAGNNNCTG 2 cut(s) 275, 287
PsuI RGATCY 2 cut(s) 112, 402
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SaqAI TTAA 2 cut(s) 75, 252
SatI GCNGC 4 cut(s) 215, 285, 447, 548
Sau3AI GATC 2 cut(s) 112, 402
Sau96I GGNCC 2 cut(s) 540, 575
SduI GDGCHC 1 cut(s) 73
SfoI GGCGCC 1 cut(s) 36
Sfr274I CTCGAG 1 cut(s) 408
SinI GGWCC 1 cut(s) 575
SlaI CTCGAG 1 cut(s) 408
SmlI CTYRAG 2 cut(s) 209, 408
SmoI CTYRAG 2 cut(s) 209, 408
SspDI GGCGCC 1 cut(s) 34
SspMI CTAG 2 cut(s) 233, 601
TaaI ACNGT 1 cut(s) 388
TaiI ACGT 2 cut(s) 101, 556
TaqI TCGA 2 cut(s) 15, 409
TfiI GAWTC 2 cut(s) 28, 425
Tru1I TTAA 2 cut(s) 75, 252
Tru9I TTAA 2 cut(s) 75, 252
TscAI CASTG 2 cut(s) 280, 391
TseFI GTSAC 5 cut(s) 53, 94, 371, 382, 388
TseI GCWGC 4 cut(s) 214, 284, 446, 547
Tsp45I GTSAC 5 cut(s) 53, 94, 371, 382, 388
TspDTI ATGAA 3 cut(s) 333, 392, 447
TspRI CASTG 2 cut(s) 280, 391
VpaK11BI GGWCC 1 cut(s) 575
XhoI CTCGAG 1 cut(s) 408
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 2 cut(s) 233, 601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.