FvH4_3g04070

Nucleolar protein 12 (25kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011265
Physical Location & Seq
Reverse (-)
2273592 .. 2276556
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g04070.t1

Sequence Viewer

Length: 585 bp
ATGGCAGCAGACAAGGCGGAAGACGCGGCGATTCCGGCGGCGGCTCCGCGTGGCCGTTACGTCAGCAAGAGGGTTCTTAAGAACAAAGCCGTCGCCGTCACCTTCAACGAGAAAAGTCTCAGTGACTTTGTGTCTGGGTTTCACAAGAGGAAGAAGAAGAGGAGAAAGGAGGCTGAGAAGAAGCAAGGGGAGGCTTTGAGGCGTAAGCTTCTTGAGCTCCGCAAAAAGAGGAGACTAGAAAAGGAATTGGCTCTCTTCGGAGGAGCTCCCCCAACTGCTGAGGGAGAAGCTGACGTGAATGATGAGGAAGATCTCATTGCTGACTATCTTGGTACAACAACGTATGATAATGGTGACATAAAAGTCACAGTGACAACCAGCGAGATCTCTCAAGAAGAGGAAATCCATCCAGTAAAGAAGACAGAGGCTGTAGTGCCAGAAACACTTGGAGCTAATAAAAAGAACAATGTTCCTGTTAGCAAAAAGAAACCTTTCAAAAAAGTTGGAAAACGCAAATCAGGGCCAAAGATGCAAAAGAAAAGAGATAAGAGGAAGGGAAAGGAAAATGGCTCGAAGAGGCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop25

Protein Analysis

195

Amino Acids

21.74

Weight (kDa)

10.08

Isoelectric Point (pI)

55.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nop25 PF09805 24 - 99 1e-09 Nucleolar protein 12 (25kDa)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11240
fragaria_vesca FvH4_1g04320 FvH4_1g04320 FvH4_3g04070
malus_domestica MD05G1325000.v1.1 MD10G1302800.v1.1
prunus_persica Prupe.4G036300_v2.0.a1
pyrus_communis pycom05g29910 pycom10g25540
rosa_chinensis RchiOBHm_Chr5g0006551
rosa_laevigata RLG00000031398
rosa_multiflora Rmu_sc0008991.1_g000017
rosa_roxburghii Rroxscaffold_1G00069510
rosa_rugosa Rorug04G0422100 Rorug04G0435300
rosa_samantha Rh5AG049600 Rh5BG048900 Rh5CG057200 Rh5DG048200
rosa_wichuraiana Rw5G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 362
AccII CGCG 2 cut(s) 26, 49
AciI CCGC 7 cut(s) 17, 26, 38, 41, 47, 220, 580
AcoI YGGCCR 1 cut(s) 52
AfaI GTAC 1 cut(s) 334
AflII CTTAAG 1 cut(s) 77
AgsI TTSAA 2 cut(s) 106, 496
AhdI GACNNNNNGTC 1 cut(s) 130
AjiI CACGTC 1 cut(s) 295
AluBI AGCT 5 cut(s) 208, 217, 266, 290, 452
AluI AGCT 5 cut(s) 208, 217, 266, 290, 452
Alw21I GWGCWC 2 cut(s) 219, 268
Alw26I GTCTC 2 cut(s) 122, 226
AlwNI CAGNNNCTG 1 cut(s) 428
AoxI GGCC 2 cut(s) 52, 521
ApeKI GCWGC 1 cut(s) 5
Asp700I GAANNNNTTC 1 cut(s) 491
AspS9I GGNCC 1 cut(s) 521
AsuHPI GGTGA 2 cut(s) 91, 365
BanII GRGCYC 2 cut(s) 219, 268
BbsI GAAGAC 2 cut(s) 27, 425
Bbv12I GWGCWC 2 cut(s) 219, 268
BbvCI CCTCAGC 1 cut(s) 279
BbvI GCAGC 1 cut(s) 17
BccI CCATC 1 cut(s) 414
BceAI ACGGC 3 cut(s) 39, 74, 80
BcoDI GTCTC 2 cut(s) 122, 226
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 429
BfrI CTTAAG 1 cut(s) 77
BglII AGATCT 2 cut(s) 310, 384
BisI GCNGC 4 cut(s) 6, 27, 39, 42
BlsI GCNGC 4 cut(s) 7, 28, 40, 43
BmeRI GACNNNNNGTC 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 295
BmgT120I GGNCC 1 cut(s) 521
BmiI GGNNCC 1 cut(s) 45
BmsI GCATC 1 cut(s) 519
BpiI GAAGAC 2 cut(s) 27, 425
Bpu10I CCTNAGC 1 cut(s) 279
BpuEI CTTGAG 2 cut(s) 233, 375
Bse1I ACTGG 1 cut(s) 410
Bse3DI GCAATG 1 cut(s) 315
BseGI GGATG 1 cut(s) 406
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 3 cut(s) 133, 165, 270
BseNI ACTGG 1 cut(s) 410
BseRI GAGGAG 3 cut(s) 175, 244, 276
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 2 cut(s) 26, 49
BshFI GGCC 2 cut(s) 54, 523
BsiHKAI GWGCWC 2 cut(s) 219, 268
BsiSI CCGG 1 cut(s) 35
BsmAI GTCTC 2 cut(s) 122, 226
BsnI GGCC 2 cut(s) 54, 523
Bsp1286I GDGCHC 2 cut(s) 219, 268
Bsp143I GATC 2 cut(s) 310, 384
BspACI CCGC 7 cut(s) 17, 26, 38, 41, 47, 220, 580
BspANI GGCC 2 cut(s) 54, 523
BspCNI CTCAG 3 cut(s) 132, 166, 271
BspFNI CGCG 2 cut(s) 26, 49
BspLI GGNNCC 1 cut(s) 45
BspTI CTTAAG 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 315
BsrI ACTGG 1 cut(s) 410
BssMI GATC 2 cut(s) 310, 384
Bst4CI ACNGT 1 cut(s) 370
Bst6I CTCTTC 4 cut(s) 152, 260, 390, 569
BstAFI CTTAAG 1 cut(s) 77
BstDEI CTNAG 3 cut(s) 119, 174, 279
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 2 cut(s) 26, 49
BstKTI GATC 2 cut(s) 313, 387
BstMAI GTCTC 2 cut(s) 122, 226
BstMBI GATC 2 cut(s) 310, 384
BstMWI GCNNNNNNNGC 5 cut(s) 14, 23, 35, 214, 529
BstSFI CTRYAG 1 cut(s) 429
BstUI CGCG 2 cut(s) 26, 49
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 2 cut(s) 27, 425
BstX2I RGATCY 2 cut(s) 310, 384
BstYI RGATCY 2 cut(s) 310, 384
BsuRI GGCC 2 cut(s) 54, 523
BtrI CACGTC 1 cut(s) 295
BtsCI GGATG 1 cut(s) 406
BtsIMutI CAGTG 2 cut(s) 127, 375
CaiI CAGNNNCTG 1 cut(s) 428
Cfr13I GGNCC 1 cut(s) 521
CseI GACGC 1 cut(s) 32
Csp6I GTAC 1 cut(s) 333
CviQI GTAC 1 cut(s) 333
DdeI CTNAG 3 cut(s) 119, 174, 279
DpnI GATC 2 cut(s) 312, 386
DpnII GATC 2 cut(s) 310, 384
DrdI GACNNNNNNGTC 1 cut(s) 362
DriI GACNNNNNGTC 1 cut(s) 130
DseDI GACNNNNNNGTC 1 cut(s) 362
EaeI YGGCCR 1 cut(s) 52
Eam1104I CTCTTC 4 cut(s) 152, 260, 390, 569
Eam1105I GACNNNNNGTC 1 cut(s) 130
EarI CTCTTC 4 cut(s) 152, 260, 390, 569
EciI GGCGGA 1 cut(s) 32
Ecl136II GAGCTC 2 cut(s) 217, 266
Eco24I GRGCYC 2 cut(s) 219, 268
Eco53kI GAGCTC 2 cut(s) 217, 266
EcoICRI GAGCTC 2 cut(s) 217, 266
EcoT38I GRGCYC 2 cut(s) 219, 268
FaiI YATR 2 cut(s) 345, 359
Fnu4HI GCNGC 4 cut(s) 6, 27, 39, 42
FokI GGATG 1 cut(s) 393
FriOI GRGCYC 2 cut(s) 219, 268
Fsp4HI GCNGC 4 cut(s) 6, 27, 39, 42
FspBI CTAG 1 cut(s) 236
GluI GCNGC 4 cut(s) 6, 27, 39, 42
HaeIII GGCC 2 cut(s) 54, 523
HapII CCGG 1 cut(s) 35
HgaI GACGC 1 cut(s) 32
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 1 cut(s) 31
HpaII CCGG 1 cut(s) 35
HphI GGTGA 2 cut(s) 91, 365
Hpy188I TCNGA 1 cut(s) 260
Hpy188III TCNNGA 2 cut(s) 212, 392
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 2 cut(s) 112, 547
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 3 cut(s) 60, 294, 341
HpyCH4V TGCA 1 cut(s) 532
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 23, 35, 214, 529
HpyF3I CTNAG 3 cut(s) 119, 174, 279
HpySE526I ACGT 3 cut(s) 60, 294, 341
Kzo9I GATC 2 cut(s) 310, 384
LmnI GCTCC 5 cut(s) 49, 222, 263, 271, 449
LpnPI CCDG 7 cut(s) 48, 120, 391, 423, 450, 486, 504
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 519
MaeI CTAG 1 cut(s) 236
MaeII ACGT 3 cut(s) 60, 294, 341
MaeIII GTNAC 6 cut(s) 56, 97, 122, 353, 364, 370
MalI GATC 2 cut(s) 312, 386
MboI GATC 2 cut(s) 310, 384
MboII GAAGA 9 cut(s) 32, 163, 166, 169, 190, 247, 320, 407, 430
MflI RGATCY 2 cut(s) 310, 384
MhlI GDGCHC 2 cut(s) 219, 268
MluCI AATT 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 484
MroXI GAANNNNTTC 1 cut(s) 491
MseI TTAA 1 cut(s) 78
MspCI CTTAAG 1 cut(s) 77
MspI CCGG 1 cut(s) 35
MvnI CGCG 2 cut(s) 26, 49
MwoI GCNNNNNNNGC 5 cut(s) 14, 23, 35, 214, 529
NdeII GATC 2 cut(s) 310, 384
NlaIV GGNNCC 1 cut(s) 45
NmuCI GTSAC 5 cut(s) 97, 122, 353, 364, 370
PdmI GAANNNNTTC 1 cut(s) 491
PfeI GAWTC 1 cut(s) 31
PkrI GCNGC 4 cut(s) 7, 28, 40, 43
Psp124BI GAGCTC 2 cut(s) 219, 268
PspN4I GGNNCC 1 cut(s) 45
PspPI GGNCC 1 cut(s) 521
PstNI CAGNNNCTG 1 cut(s) 428
PsuI RGATCY 2 cut(s) 310, 384
RsaI GTAC 1 cut(s) 334
RsaNI GTAC 1 cut(s) 333
SacI GAGCTC 2 cut(s) 219, 268
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 4 cut(s) 6, 27, 39, 42
Sau3AI GATC 2 cut(s) 310, 384
Sau96I GGNCC 1 cut(s) 521
SduI GDGCHC 2 cut(s) 219, 268
SfaNI GCATC 1 cut(s) 519
SfcI CTRYAG 1 cut(s) 429
SmlI CTYRAG 3 cut(s) 77, 212, 390
SmoI CTYRAG 3 cut(s) 77, 212, 390
Sse9I AATT 1 cut(s) 245
SsiI CCGC 7 cut(s) 17, 26, 38, 41, 47, 220, 580
SspMI CTAG 1 cut(s) 236
SstI GAGCTC 2 cut(s) 219, 268
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 3 cut(s) 63, 297, 344
TaqI TCGA 1 cut(s) 572
TasI AATT 1 cut(s) 245
TauI GCSGC 3 cut(s) 29, 41, 44
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 2 cut(s) 127, 375
TseFI GTSAC 5 cut(s) 97, 122, 353, 364, 370
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 5 cut(s) 97, 122, 353, 364, 370
TspRI CASTG 2 cut(s) 127, 375
Vha464I CTTAAG 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 491
XspI CTAG 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.