Rw5G004970

Nucleolar protein 12 (25kDa)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011265
Physical Location & Seq
Reverse (-)
5160820 .. 5162462
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G004970.1

Sequence Viewer

Length: 564 bp
ATGGCAGCAGACAAGGCTGAAGACTTGGCGATTCCGGCCGCCGCTCCCCGCGGCCGTTACATCAACAAGAGGGCTCTTAAAAACAAGGCCGTCGCCAGGAAGAAGAAGAGGAGAAAGGAAGCTGAGAAGAAGCAAGGGGAGGCTTTAAGGCGGAAGCTTCTTGAGCTCCGCAAAAAGAGGAGACTAGAAAAGGAATTGGCTCTCTTTGGTGGAGCTCCCCCAGCTGCTGAGGGAGAAGCTGACGAGAATGATGAGGGAGATGTGAGCCAGGAGGAAGATGAAGAAAGTGAGCCAGATGCACCAATTTCTGGTACAACAACTTACGACAATGGAGACATAAAAGTCACAGTGACGACAAGCGAGATCTCTCAGGAAGAGGAAATACATCCAGTTAGAAAGACAGAGGCTGTAGTGCCCGAAACAGTTGGAGCTAATAAAAAGAACAGTGTTCCTGTGATAAATAAGAAACCATTTAAAAAAGTTGGAAAGCGCAAATCAGGGCCAAAGATGCAAAAGAAAAGAGATAAAAGGAAAGGGAAGGAAAATAACTCGAAGAGGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Nop25

Protein Analysis

187

Amino Acids

20.91

Weight (kDa)

9.86

Isoelectric Point (pI)

66.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011265)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11240
fragaria_vesca FvH4_1g04320 FvH4_1g04320 FvH4_3g04070
malus_domestica MD05G1325000.v1.1 MD10G1302800.v1.1
prunus_persica Prupe.4G036300_v2.0.a1
pyrus_communis pycom05g29910 pycom10g25540
rosa_chinensis RchiOBHm_Chr5g0006551
rosa_laevigata RLG00000031398
rosa_multiflora Rmu_sc0008991.1_g000017
rosa_roxburghii Rroxscaffold_1G00069510
rosa_rugosa Rorug04G0422100 Rorug04G0435300
rosa_samantha Rh5AG049600 Rh5BG048900 Rh5CG057200 Rh5DG048200
rosa_wichuraiana Rw5G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 341
AccB7I CCANNNNNTGG 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 44
AccII CGCG 1 cut(s) 51
AciI CCGC 6 cut(s) 39, 42, 49, 51, 151, 169
AcoI YGGCCR 2 cut(s) 36, 52
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 1 cut(s) 313
AfiI CCNNNNNNNGG 2 cut(s) 96, 308
AjnI CCWGG 2 cut(s) 95, 267
AluBI AGCT 7 cut(s) 122, 157, 166, 215, 224, 239, 431
AluI AGCT 7 cut(s) 122, 157, 166, 215, 224, 239, 431
Alw21I GWGCWC 2 cut(s) 168, 217
Alw26I GTCTC 2 cut(s) 175, 327
AlwNI CAGNNNCTG 2 cut(s) 227, 407
AoxI GGCC 4 cut(s) 36, 52, 87, 500
ApeKI GCWGC 2 cut(s) 5, 224
AspLEI GCGC 2 cut(s) 492, 561
AspS9I GGNCC 1 cut(s) 500
BaeGI GKGCMC 1 cut(s) 417
BanII GRGCYC 3 cut(s) 76, 168, 217
BarI GAAGNNNNNNTAC 2 cut(s) 366, 398
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 2 cut(s) 168, 217
BbvCI CCTCAGC 1 cut(s) 228
BbvI GCAGC 2 cut(s) 17, 211
BceAI ACGGC 2 cut(s) 39, 74
BciT130I CCWGG 2 cut(s) 97, 269
BcoDI GTCTC 2 cut(s) 175, 327
BfaI CTAG 2 cut(s) 185, 562
BfmI CTRYAG 1 cut(s) 408
BfoI RGCGCY 1 cut(s) 562
BglII AGATCT 1 cut(s) 363
BisI GCNGC 5 cut(s) 6, 39, 42, 52, 225
BlsI GCNGC 5 cut(s) 7, 40, 43, 53, 226
Bme1390I CCNGG 2 cut(s) 97, 269
BmgT120I GGNCC 1 cut(s) 500
BmrFI CCNGG 2 cut(s) 97, 269
BmsI GCATC 2 cut(s) 286, 498
BpiI GAAGAC 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 308
Bse1I ACTGG 1 cut(s) 389
BseBI CCWGG 2 cut(s) 97, 269
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 385
BseLI CCNNNNNNNGG 2 cut(s) 96, 308
BseMII CTCAG 3 cut(s) 114, 219, 383
BseNI ACTGG 1 cut(s) 389
BseRI GAGGAG 2 cut(s) 124, 193
BseSI GKGCMC 1 cut(s) 417
BseX3I CGGCCG 2 cut(s) 36, 52
BseXI GCAGC 2 cut(s) 17, 211
BseYI CCCAGC 1 cut(s) 220
Bsh1236I CGCG 1 cut(s) 51
Bsh1285I CGRYCG 2 cut(s) 39, 55
BshFI GGCC 4 cut(s) 38, 54, 89, 502
BsiEI CGRYCG 2 cut(s) 39, 55
BsiHKAI GWGCWC 2 cut(s) 168, 217
BsiSI CCGG 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 96, 308
BsmAI GTCTC 2 cut(s) 175, 327
BsnI GGCC 4 cut(s) 38, 54, 89, 502
Bsp1286I GDGCHC 4 cut(s) 76, 168, 217, 417
Bsp143I GATC 1 cut(s) 363
BspACI CCGC 6 cut(s) 39, 42, 49, 51, 151, 169
BspANI GGCC 4 cut(s) 38, 54, 89, 502
BspCNI CTCAG 3 cut(s) 115, 220, 382
BspFNI CGCG 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 44
BsrI ACTGG 1 cut(s) 389
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 1 cut(s) 363
Bst2UI CCWGG 2 cut(s) 97, 269
Bst4CI ACNGT 3 cut(s) 349, 424, 446
Bst6I CTCTTC 3 cut(s) 101, 369, 548
BstDEI CTNAG 3 cut(s) 123, 228, 369
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 385
BstFNI CGCG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 562
BstHHI GCGC 2 cut(s) 492, 561
BstKTI GATC 1 cut(s) 366
BstMAI GTCTC 2 cut(s) 175, 327
BstMBI GATC 1 cut(s) 363
BstMCI CGRYCG 2 cut(s) 39, 55
BstMWI GCNNNNNNNGC 5 cut(s) 14, 35, 163, 221, 508
BstNI CCWGG 2 cut(s) 97, 269
BstSCI CCNGG 2 cut(s) 95, 267
BstSFI CTRYAG 1 cut(s) 408
BstSLI GKGCMC 1 cut(s) 417
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 17, 211
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 363
BstYI RGATCY 1 cut(s) 363
BstZI CGGCCG 2 cut(s) 36, 52
BsuRI GGCC 4 cut(s) 38, 54, 89, 502
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 385
BtsIMutI CAGTG 2 cut(s) 354, 451
CaiI CAGNNNCTG 2 cut(s) 227, 407
CfoI GCGC 2 cut(s) 492, 561
Cfr13I GGNCC 1 cut(s) 500
Cfr42I CCGCGG 1 cut(s) 52
Csp6I GTAC 1 cut(s) 312
CviQI GTAC 1 cut(s) 312
DdeI CTNAG 3 cut(s) 123, 228, 369
DpnI GATC 1 cut(s) 365
DpnII GATC 1 cut(s) 363
DraI TTTAAA 1 cut(s) 475
DrdI GACNNNNNNGTC 1 cut(s) 341
DseDI GACNNNNNNGTC 1 cut(s) 341
EaeI YGGCCR 2 cut(s) 36, 52
EagI CGGCCG 2 cut(s) 36, 52
Eam1104I CTCTTC 3 cut(s) 101, 369, 548
EarI CTCTTC 3 cut(s) 101, 369, 548
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 2 cut(s) 166, 215
EclXI CGGCCG 2 cut(s) 36, 52
Eco24I GRGCYC 3 cut(s) 76, 168, 217
Eco52I CGGCCG 2 cut(s) 36, 52
Eco53kI GAGCTC 2 cut(s) 166, 215
Eco57I CTGAAG 1 cut(s) 39
EcoICRI GAGCTC 2 cut(s) 166, 215
EcoRII CCWGG 2 cut(s) 95, 267
EcoT38I GRGCYC 3 cut(s) 76, 168, 217
FaiI YATR 1 cut(s) 338
FauI CCCGC 1 cut(s) 56
Fnu4HI GCNGC 5 cut(s) 6, 39, 42, 52, 225
FokI GGATG 1 cut(s) 372
FriOI GRGCYC 3 cut(s) 76, 168, 217
Fsp4HI GCNGC 5 cut(s) 6, 39, 42, 52, 225
FspBI CTAG 2 cut(s) 185, 562
GlaI GCGC 2 cut(s) 491, 560
GluI GCNGC 5 cut(s) 6, 39, 42, 52, 225
GsaI CCCAGC 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 562
HaeIII GGCC 4 cut(s) 38, 54, 89, 502
HapII CCGG 1 cut(s) 35
HhaI GCGC 2 cut(s) 492, 561
Hin6I GCGC 2 cut(s) 490, 559
HinP1I GCGC 2 cut(s) 490, 559
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 1 cut(s) 31
HpaII CCGG 1 cut(s) 35
Hpy188III TCNNGA 2 cut(s) 161, 371
Hpy99I CGWCG 1 cut(s) 95
HpyAV CCTTC 1 cut(s) 532
HpyCH4III ACNGT 3 cut(s) 349, 424, 446
HpyCH4V TGCA 2 cut(s) 299, 511
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 35, 163, 221, 508
HpyF3I CTNAG 3 cut(s) 123, 228, 369
HspAI GCGC 2 cut(s) 490, 559
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 1 cut(s) 363
LmnI GCTCC 5 cut(s) 49, 171, 212, 220, 428
Lsp1109I GCAGC 2 cut(s) 17, 211
LweI GCATC 2 cut(s) 286, 498
MaeI CTAG 2 cut(s) 185, 562
MaeIII GTNAC 3 cut(s) 56, 343, 349
MalI GATC 1 cut(s) 365
MbiI CCGCTC 1 cut(s) 44
MboI GATC 1 cut(s) 363
MboII GAAGA 8 cut(s) 32, 112, 115, 118, 139, 287, 293, 386
MflI RGATCY 1 cut(s) 363
MhlI GDGCHC 4 cut(s) 76, 168, 217, 417
MluCI AATT 2 cut(s) 194, 303
MmeI TCCRAC 2 cut(s) 406, 463
MseI TTAA 3 cut(s) 78, 146, 474
MspA1I CMGCKG 2 cut(s) 51, 224
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 2 cut(s) 97, 269
MvaI CCWGG 2 cut(s) 97, 269
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 5 cut(s) 14, 35, 163, 221, 508
NdeII GATC 1 cut(s) 363
NmuCI GTSAC 2 cut(s) 343, 349
PfeI GAWTC 1 cut(s) 31
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 5 cut(s) 7, 40, 43, 53, 226
Psp124BI GAGCTC 2 cut(s) 168, 217
Psp6I CCWGG 2 cut(s) 95, 267
PspFI CCCAGC 1 cut(s) 220
PspGI CCWGG 2 cut(s) 95, 267
PspPI GGNCC 1 cut(s) 500
PstNI CAGNNNCTG 2 cut(s) 227, 407
PsuI RGATCY 1 cut(s) 363
PvuII CAGCTG 1 cut(s) 224
RsaI GTAC 1 cut(s) 313
RsaNI GTAC 1 cut(s) 312
SacI GAGCTC 2 cut(s) 168, 217
SacII CCGCGG 1 cut(s) 52
SaqAI TTAA 3 cut(s) 78, 146, 474
SatI GCNGC 5 cut(s) 6, 39, 42, 52, 225
Sau3AI GATC 1 cut(s) 363
Sau96I GGNCC 1 cut(s) 500
ScrFI CCNGG 2 cut(s) 97, 269
SduI GDGCHC 4 cut(s) 76, 168, 217, 417
SetI ASST 7 cut(s) 124, 159, 168, 217, 226, 241, 433
SfaNI GCATC 2 cut(s) 286, 498
SfcI CTRYAG 1 cut(s) 408
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 2 cut(s) 194, 303
SsiI CCGC 6 cut(s) 39, 42, 49, 51, 151, 169
SspMI CTAG 2 cut(s) 185, 562
SstI GAGCTC 2 cut(s) 168, 217
StyD4I CCNGG 2 cut(s) 95, 267
TaaI ACNGT 3 cut(s) 349, 424, 446
TaqI TCGA 1 cut(s) 551
TasI AATT 2 cut(s) 194, 303
TauI GCSGC 3 cut(s) 41, 44, 54
TfiI GAWTC 1 cut(s) 31
Tru1I TTAA 3 cut(s) 78, 146, 474
Tru9I TTAA 3 cut(s) 78, 146, 474
TscAI CASTG 2 cut(s) 354, 451
TseFI GTSAC 2 cut(s) 343, 349
TseI GCWGC 2 cut(s) 5, 224
Tsp45I GTSAC 2 cut(s) 343, 349
TspDTI ATGAA 1 cut(s) 294
TspRI CASTG 2 cut(s) 354, 451
Van91I CCANNNNNTGG 1 cut(s) 308
XspI CTAG 2 cut(s) 185, 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.