FvH4_1g06500

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012044
Physical Location & Seq
Forward (+)
3428995 .. 3430935
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06500.t1

Sequence Viewer

Length: 762 bp
ATGGCCTCCGCCTTCCTCCTCCTCCTCATCACCATCCCCGCCGCGTTATCTCATTCGATCCTCCAATTCCCGCACGTCTCCGACTACGACCGGAAGCTCCGCGCCGACGACAACATCTACTGCGACAGCTGGCGATTCTCCGTCGAGACCAACGACGCCGGGAGCTGGGACAGCATTCCCTCCCGGTGCGTCGCGTTCGTCCAGGACTACATGACCGGCGACCGCTACGCCTCCGACTCGGCGATCGTCTCGAATTTCTCGCTGGCGTTCGGCCAGGGCGTGAAGCTCGGCGGCGACGGGAAAGACTCCTGGGTTTTCGATATCGATGAGACGCTGCTATCCAATTTGCCGTACTATCAGGAGCACGGCTTCGGAGCAGTGACATTCGATGAGGTATCTTTTGATAAGTGGGTGGATTTGGCTGAGGCCCCTGCTATCCCAGCAAGTTTGAGTCTGTACAAGGGGCTCCAACGGTTGGGGTTCAAGATTTTCCTGTTAACTGGGCGGAGCGAATTTCAGAGAAATGCCACGGTGAACAACCTCGTATATTCAGGTTACAGCGACTGGGAGAGGCTGCTACTGAGGGGACCTTCAGATAAAGGAACACTAGCGACTGAATACAAATCCAAGAAGAGAGCAGAACTGATAAACGAAGGTTTCCGAATTCATGGGAGCTCGGGAGATCAATGGAGTGACTTGTTGGGCTTTGCAGTCGCCCGACGATCCTTTAAACTTCCAAACCCAATGTACTATATTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

254

Amino Acids

28.35

Weight (kDa)

5.25

Isoelectric Point (pI)

34.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 36 - 252 5.7e-77 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29260 AT4G29270
fragaria_vesca FvH4_1g06500
malus_domestica MD02G1068300.v1.1
prunus_persica Prupe.7G217300_v2.0.a1
pyrus_communis pycom02g05410 pycom08g17150 pycom15g17740
rosa_chinensis RchiOBHm_Chr2g0092251
rosa_laevigata RLG00000016290
rosa_multiflora Rmu_sc0000524.1_g000032 Rmu_sc0030540.1_g000001
rosa_roxburghii Rroxscaffold_2G00149330
rosa_rugosa Rorug02G0024100
rosa_samantha Rh2AG069900 Rh2BG069400 Rh2CG070600 Rh2DG068400
rosa_wichuraiana Rw2G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 475
AccII CGCG 3 cut(s) 44, 102, 194
AciI CCGC 8 cut(s) 9, 39, 42, 71, 100, 223, 291, 505
AclWI GGATC 2 cut(s) 52, 717
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 3 cut(s) 253, 512, 663
AcuI CTGAAG 1 cut(s) 576
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 3 cut(s) 353, 458, 749
AfiI CCNNNNNNNGG 2 cut(s) 165, 475
AgsI TTSAA 1 cut(s) 484
AjiI CACGTC 1 cut(s) 76
AjnI CCWGG 3 cut(s) 201, 273, 308
AluBI AGCT 5 cut(s) 97, 129, 165, 286, 675
AluI AGCT 5 cut(s) 97, 129, 165, 286, 675
Alw21I GWGCWC 2 cut(s) 366, 677
Alw26I GTCTC 4 cut(s) 82, 140, 253, 323
AlwI GGATC 2 cut(s) 52, 717
AlwNI CAGNNNCTG 1 cut(s) 564
Ama87I CYCGRG 1 cut(s) 676
AoxI GGCC 3 cut(s) 3, 271, 426
ApeKI GCWGC 2 cut(s) 334, 574
ApoI RAATTY 3 cut(s) 253, 512, 663
AspLEI GCGC 1 cut(s) 104
AspS9I GGNCC 2 cut(s) 427, 587
AsuC2I CCSGG 2 cut(s) 160, 184
AsuHPI GGTGA 2 cut(s) 22, 544
AvaI CYCGRG 1 cut(s) 676
AvaII GGWCC 1 cut(s) 587
BanII GRGCYC 2 cut(s) 468, 677
Bbv12I GWGCWC 2 cut(s) 366, 677
BbvCI CCTCAGC 1 cut(s) 423
BbvI GCAGC 2 cut(s) 321, 561
BccI CCATC 1 cut(s) 41
BceAI ACGGC 2 cut(s) 334, 382
BciT130I CCWGG 3 cut(s) 203, 275, 310
BcnI CCSGG 2 cut(s) 160, 184
BcoDI GTCTC 4 cut(s) 82, 140, 253, 323
BfaI CTAG 1 cut(s) 608
BisI GCNGC 4 cut(s) 42, 292, 335, 575
BlsI GCNGC 4 cut(s) 43, 293, 336, 576
Bme1390I CCNGG 5 cut(s) 160, 184, 203, 275, 310
Bme18I GGWCC 1 cut(s) 587
BmeT110I CYCGRG 1 cut(s) 676
BmgBI CACGTC 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 427, 587
BmiI GGNNCC 3 cut(s) 429, 467, 588
BmrFI CCNGG 5 cut(s) 160, 184, 203, 275, 310
BmrI ACTGGG 2 cut(s) 510, 574
BmuI ACTGGG 2 cut(s) 510, 574
Bpu10I CCTNAGC 1 cut(s) 423
BpuMI CCSGG 2 cut(s) 160, 184
Bsa29I ATCGAT 1 cut(s) 324
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 140
BsaJI CCNNGG 3 cut(s) 274, 309, 528
BsaWI WCCGGW 1 cut(s) 90
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 475
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 505, 569
BseBI CCWGG 3 cut(s) 203, 275, 310
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 3 cut(s) 274, 309, 528
BseGI GGATG 1 cut(s) 33
BseLI CCNNNNNNNGG 2 cut(s) 165, 475
BseMII CTCAG 2 cut(s) 414, 572
BseNI ACTGG 2 cut(s) 505, 569
BseRI GAGGAG 3 cut(s) 8, 11, 14
BseXI GCAGC 2 cut(s) 321, 561
BseYI CCCAGC 2 cut(s) 165, 439
Bsh1236I CGCG 3 cut(s) 44, 102, 194
Bsh1285I CGRYCG 3 cut(s) 91, 223, 246
BshFI GGCC 3 cut(s) 5, 273, 428
BshVI ATCGAT 1 cut(s) 324
BsiEI CGRYCG 3 cut(s) 91, 223, 246
BsiHKAI GWGCWC 2 cut(s) 366, 677
BsiHKCI CYCGRG 1 cut(s) 676
BsiSI CCGG 4 cut(s) 91, 159, 184, 216
BslFI GGGAC 2 cut(s) 182, 600
BslI CCNNNNNNNGG 2 cut(s) 165, 475
BsmAI GTCTC 4 cut(s) 82, 140, 253, 323
BsmBI CGTCTC 3 cut(s) 82, 253, 323
BsmFI GGGAC 2 cut(s) 182, 600
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 3 cut(s) 5, 273, 428
Bso31I GGTCTC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 676
Bsp1286I GDGCHC 3 cut(s) 366, 468, 677
Bsp1407I TGTACA 1 cut(s) 456
Bsp143I GATC 4 cut(s) 57, 243, 682, 722
BspACI CCGC 8 cut(s) 9, 39, 42, 71, 100, 223, 291, 505
BspANI GGCC 3 cut(s) 5, 273, 428
BspCNI CTCAG 2 cut(s) 415, 573
BspDI ATCGAT 1 cut(s) 324
BspFNI CGCG 3 cut(s) 44, 102, 194
BspLI GGNNCC 3 cut(s) 429, 467, 588
BspPI GGATC 2 cut(s) 52, 717
BspTNI GGTCTC 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 456
BsrI ACTGG 2 cut(s) 505, 569
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 3 cut(s) 274, 309, 528
BssMI GATC 4 cut(s) 57, 243, 682, 722
BssNI GRCGYC 1 cut(s) 156
Bst2UI CCWGG 3 cut(s) 203, 275, 310
Bst4CI ACNGT 2 cut(s) 474, 532
Bst6I CTCTTC 1 cut(s) 626
BstACI GRCGYC 1 cut(s) 156
BstAUI TGTACA 1 cut(s) 456
BstC8I GCNNGC 2 cut(s) 131, 264
BstDEI CTNAG 2 cut(s) 423, 581
BstDSI CCRYGG 1 cut(s) 528
BstF5I GGATG 1 cut(s) 33
BstFNI CGCG 3 cut(s) 44, 102, 194
BstHHI GCGC 1 cut(s) 104
BstKTI GATC 4 cut(s) 60, 246, 685, 725
BstMAI GTCTC 4 cut(s) 82, 140, 253, 323
BstMBI GATC 4 cut(s) 57, 243, 682, 722
BstMCI CGRYCG 3 cut(s) 91, 223, 246
BstMWI GCNNNNNNNGC 2 cut(s) 171, 440
BstNI CCWGG 3 cut(s) 203, 275, 310
BstSCI CCNGG 5 cut(s) 158, 182, 201, 273, 308
BstUI CGCG 3 cut(s) 44, 102, 194
BstV1I GCAGC 2 cut(s) 321, 561
Bsu15I ATCGAT 1 cut(s) 324
BsuRI GGCC 3 cut(s) 5, 273, 428
BsuTUI ATCGAT 1 cut(s) 324
BtgI CCRYGG 1 cut(s) 528
BtrI CACGTC 1 cut(s) 76
BtsCI GGATG 1 cut(s) 33
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 1 cut(s) 384
Cac8I GCNNGC 2 cut(s) 131, 264
CaiI CAGNNNCTG 1 cut(s) 564
CfoI GCGC 1 cut(s) 104
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 2 cut(s) 427, 587
ClaI ATCGAT 1 cut(s) 324
CseI GACGC 3 cut(s) 164, 178, 340
Csp6I GTAC 3 cut(s) 352, 457, 748
CviAII CATG 2 cut(s) 211, 668
CviQI GTAC 3 cut(s) 352, 457, 748
DdeI CTNAG 2 cut(s) 423, 581
DpnI GATC 4 cut(s) 59, 245, 684, 724
DpnII GATC 4 cut(s) 57, 243, 682, 722
DraI TTTAAA 1 cut(s) 730
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 626
EarI CTCTTC 1 cut(s) 626
EciI GGCGGA 1 cut(s) 520
Ecl136II GAGCTC 1 cut(s) 675
Eco24I GRGCYC 2 cut(s) 468, 677
Eco31I GGTCTC 1 cut(s) 140
Eco32I GATATC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 587
Eco53kI GAGCTC 1 cut(s) 675
Eco57I CTGAAG 1 cut(s) 576
Eco88I CYCGRG 1 cut(s) 676
EcoICRI GAGCTC 1 cut(s) 675
EcoO109I RGGNCCY 2 cut(s) 427, 587
EcoRI GAATTC 1 cut(s) 663
EcoRII CCWGG 3 cut(s) 201, 273, 308
EcoRV GATATC 1 cut(s) 322
EcoT38I GRGCYC 2 cut(s) 468, 677
Esp3I CGTCTC 3 cut(s) 82, 253, 323
FaeI CATG 2 cut(s) 214, 671
FaiI YATR 4 cut(s) 212, 547, 669, 753
FaqI GGGAC 2 cut(s) 182, 600
FatI CATG 2 cut(s) 210, 667
FauI CCCGC 2 cut(s) 46, 78
Fnu4HI GCNGC 4 cut(s) 42, 292, 335, 575
FokI GGATG 1 cut(s) 20
FriOI GRGCYC 2 cut(s) 468, 677
Fsp4HI GCNGC 4 cut(s) 42, 292, 335, 575
FspBI CTAG 1 cut(s) 608
GlaI GCGC 1 cut(s) 103
GluI GCNGC 4 cut(s) 42, 292, 335, 575
GsaI CCCAGC 2 cut(s) 169, 443
HaeIII GGCC 3 cut(s) 5, 273, 428
HapII CCGG 4 cut(s) 91, 159, 184, 216
HgaI GACGC 3 cut(s) 164, 178, 340
HhaI GCGC 1 cut(s) 104
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 2 cut(s) 214, 671
Hin6I GCGC 1 cut(s) 102
HinP1I GCGC 1 cut(s) 102
HincII GTYRAC 1 cut(s) 498
HindII GTYRAC 1 cut(s) 498
HinfI GANTC 4 cut(s) 135, 236, 305, 451
HpaI GTTAAC 1 cut(s) 498
HpaII CCGG 4 cut(s) 91, 159, 184, 216
HphI GGTGA 2 cut(s) 22, 544
Hpy166II GTNNAC 2 cut(s) 498, 535
Hpy188I TCNGA 6 cut(s) 82, 235, 374, 519, 595, 662
Hpy188III TCNNGA 5 cut(s) 145, 250, 359, 484, 678
Hpy8I GTNNAC 2 cut(s) 498, 535
Hpy99I CGWCG 6 cut(s) 110, 146, 158, 194, 299, 723
HpyAV CCTTC 3 cut(s) 22, 600, 647
HpyCH4III ACNGT 2 cut(s) 474, 532
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 1 cut(s) 710
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 440
HpyF3I CTNAG 2 cut(s) 423, 581
HpySE526I ACGT 1 cut(s) 75
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 2 cut(s) 214, 671
HspAI GCGC 1 cut(s) 102
KspAI GTTAAC 1 cut(s) 498
Kzo9I GATC 4 cut(s) 57, 243, 682, 722
LmnI GCTCC 7 cut(s) 102, 162, 361, 374, 471, 507, 672
Lsp1109I GCAGC 2 cut(s) 321, 561
MaeI CTAG 1 cut(s) 608
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 3 cut(s) 379, 554, 692
MalI GATC 4 cut(s) 59, 245, 684, 724
MboI GATC 4 cut(s) 57, 243, 682, 722
MboII GAAGA 1 cut(s) 643
MhlI GDGCHC 3 cut(s) 366, 468, 677
MluCI AATT 5 cut(s) 65, 253, 343, 512, 663
MlyI GAGTC 3 cut(s) 230, 299, 460
MmeI TCCRAC 3 cut(s) 105, 258, 493
MseI TTAA 2 cut(s) 497, 729
MspA1I CMGCKG 1 cut(s) 129
MspI CCGG 4 cut(s) 91, 159, 184, 216
MspR9I CCNGG 5 cut(s) 160, 184, 203, 275, 310
Mva1269I GAATGC 1 cut(s) 174
MvaI CCWGG 3 cut(s) 203, 275, 310
MvnI CGCG 3 cut(s) 44, 102, 194
MwoI GCNNNNNNNGC 2 cut(s) 171, 440
NciI CCSGG 2 cut(s) 160, 184
NdeII GATC 4 cut(s) 57, 243, 682, 722
NlaIII CATG 2 cut(s) 214, 671
NlaIV GGNNCC 3 cut(s) 429, 467, 588
NmeAIII GCCGAG 2 cut(s) 218, 267
NmuCI GTSAC 2 cut(s) 379, 692
PcsI WCGNNNNNNNCGW 2 cut(s) 150, 276
PctI GAATGC 1 cut(s) 174
PfeI GAWTC 1 cut(s) 135
PflMI CCANNNNNTGG 1 cut(s) 475
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 4 cut(s) 43, 293, 336, 576
Ple19I CGATCG 1 cut(s) 246
PleI GAGTC 3 cut(s) 230, 299, 459
PpsI GAGTC 3 cut(s) 230, 299, 459
PpuMI RGGWCCY 1 cut(s) 587
Psp124BI GAGCTC 1 cut(s) 677
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 3 cut(s) 201, 273, 308
PspFI CCCAGC 2 cut(s) 165, 439
PspGI CCWGG 3 cut(s) 201, 273, 308
PspN4I GGNNCC 3 cut(s) 429, 467, 588
PspPI GGNCC 2 cut(s) 427, 587
PspPPI RGGWCCY 1 cut(s) 587
PstNI CAGNNNCTG 1 cut(s) 564
PvuI CGATCG 1 cut(s) 246
PvuII CAGCTG 1 cut(s) 129
RsaI GTAC 3 cut(s) 353, 458, 749
RsaNI GTAC 3 cut(s) 352, 457, 748
SacI GAGCTC 1 cut(s) 677
SaqAI TTAA 2 cut(s) 497, 729
SatI GCNGC 4 cut(s) 42, 292, 335, 575
Sau3AI GATC 4 cut(s) 57, 243, 682, 722
Sau96I GGNCC 2 cut(s) 427, 587
SchI GAGTC 3 cut(s) 230, 299, 460
ScrFI CCNGG 5 cut(s) 160, 184, 203, 275, 310
SduI GDGCHC 3 cut(s) 366, 468, 677
SinI GGWCC 1 cut(s) 587
Sse9I AATT 5 cut(s) 65, 253, 343, 512, 663
SsiI CCGC 8 cut(s) 9, 39, 42, 71, 100, 223, 291, 505
SspMI CTAG 1 cut(s) 608
SstI GAGCTC 1 cut(s) 677
StyD4I CCNGG 5 cut(s) 158, 182, 201, 273, 308
TaaI ACNGT 2 cut(s) 474, 532
TaiI ACGT 1 cut(s) 78
TaqI TCGA 6 cut(s) 56, 144, 251, 318, 324, 387
TasI AATT 5 cut(s) 65, 253, 343, 512, 663
TatI WGTACW 2 cut(s) 456, 747
TauI GCSGC 2 cut(s) 44, 294
TfiI GAWTC 1 cut(s) 135
Tru1I TTAA 2 cut(s) 497, 729
Tru9I TTAA 2 cut(s) 497, 729
TscAI CASTG 1 cut(s) 384
TseFI GTSAC 2 cut(s) 379, 692
TseI GCWGC 2 cut(s) 334, 574
Tsp45I GTSAC 2 cut(s) 379, 692
TspDTI ATGAA 1 cut(s) 656
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 1 cut(s) 384
Van91I CCANNNNNTGG 1 cut(s) 475
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 3 cut(s) 253, 512, 663
XspI CTAG 1 cut(s) 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.