MD02G1068300.v1.1

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012044
Physical Location & Seq
Forward (+)
5551012 .. 5554025
3014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1068300.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGCCTCCGTCCGCCACCGCCTCCTCCTCCTCATCACCCTCCTCGCAATTCCCGCCTCCTTATCATCCCAATCCGTCATCCAAATGCCTAGGAAACGACACCGCTTCGCCGCCTCCGACGACAGCCTCTACTGCGACAGCTGGAGATTCTCCATCGAGACCAACGACGCCGGAACCTGGTCCAACATTCCGTCCAGGTGCATCCGATTCGTCCAGGACTACATGACCGGCGACCGGTACCGGTCCGACTTGGCGTCCGTCGCGAATTACTCGCTGAGCTTCGCCAAGGGGGTGACGATAGGCGGCGATGGGAAGGACGCGTGGGTGTTCGACATCGACGAGACTCTGCTTTCCAATTTGGCCTACTATCAGGCCCATGGATTCGGATCGGAGACCTTCAATGAAGCATTTTTCGATGAGTGGGTGGATTTGGCTGAGGCCCCTGCTCTCCCAGCAAGTTTAAATCTTTACAAAGAGCTTGAGCAGTTGGGTTTCAAGATGTTTCTGTTAACTGGGCGGAGCGAACATCAGAGAAACGCCACCGCGAGAAACCTTCTATATGCAGGACCTTCTGATCAAGGAACACCGGCCACGGTCTACAAATCCCAGAAGAGATCGGACTTGATAAATGAAGGTTATCGAATTCACGGGAGCTCGGGAGATCAATGGAGTGATTTGATAGGTTTTGCTATTGCTCAACGATCGTTTAAACTTCCAAACCCAATGTATTACATTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

247

Amino Acids

27.64

Weight (kDa)

5.96

Isoelectric Point (pI)

42.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 38 - 245 5.1e-67 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29260 AT4G29270
fragaria_vesca FvH4_1g06500
malus_domestica MD02G1068300.v1.1
prunus_persica Prupe.7G217300_v2.0.a1
pyrus_communis pycom02g05410 pycom08g17150 pycom15g17740
rosa_chinensis RchiOBHm_Chr2g0092251
rosa_laevigata RLG00000016290
rosa_multiflora Rmu_sc0000524.1_g000032 Rmu_sc0030540.1_g000001
rosa_roxburghii Rroxscaffold_2G00149330
rosa_rugosa Rorug02G0024100
rosa_samantha Rh2AG069900 Rh2BG069400 Rh2CG070600 Rh2DG068400
rosa_wichuraiana Rw2G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 237
AccB1I GGYRCC 1 cut(s) 237
AccI GTMKAC 1 cut(s) 597
AccII CGCG 3 cut(s) 263, 320, 545
AciI CCGC 8 cut(s) 13, 19, 54, 103, 111, 303, 517, 543
AclWI GGATC 1 cut(s) 394
AcoI YGGCCR 1 cut(s) 588
AcsI RAATTY 1 cut(s) 642
AcyI GRCGYC 2 cut(s) 168, 254
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 2 cut(s) 177, 234
AflIII ACRYGT 1 cut(s) 318
AgeI ACCGGT 2 cut(s) 234, 240
AgsI TTSAA 2 cut(s) 400, 496
AhdI GACNNNNNGTC 1 cut(s) 253
AjnI CCWGG 3 cut(s) 176, 194, 213
AluBI AGCT 4 cut(s) 141, 279, 478, 654
AluI AGCT 4 cut(s) 141, 279, 478, 654
Alw21I GWGCWC 1 cut(s) 656
Alw26I GTCTC 3 cut(s) 152, 335, 386
AlwI GGATC 1 cut(s) 394
Ama87I CYCGRG 1 cut(s) 655
AoxI GGCC 5 cut(s) 3, 360, 372, 438, 588
ApoI RAATTY 1 cut(s) 642
AsiGI ACCGGT 2 cut(s) 234, 240
Asp718I GGTACC 1 cut(s) 237
AspA2I CCTAGG 1 cut(s) 89
AspS9I GGNCC 5 cut(s) 180, 243, 373, 439, 566
AsuHPI GGTGA 2 cut(s) 28, 304
AvaI CYCGRG 1 cut(s) 655
AvaII GGWCC 3 cut(s) 180, 243, 566
AvrII CCTAGG 1 cut(s) 89
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 656
Bbv12I GWGCWC 1 cut(s) 656
BbvCI CCTCAGC 1 cut(s) 435
BccI CCATC 2 cut(s) 161, 302
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 3 cut(s) 178, 196, 215
BclI TGATCA 1 cut(s) 574
BcoDI GTCTC 3 cut(s) 152, 335, 386
BfaI CTAG 2 cut(s) 90, 739
BisI GCNGC 2 cut(s) 111, 304
BlnI CCTAGG 1 cut(s) 89
BlpI GCTNAGC 1 cut(s) 275
BlsI GCNGC 2 cut(s) 112, 305
Bme1390I CCNGG 3 cut(s) 178, 196, 215
Bme18I GGWCC 3 cut(s) 180, 243, 566
BmeRI GACNNNNNGTC 1 cut(s) 253
BmeT110I CYCGRG 1 cut(s) 655
BmgT120I GGNCC 5 cut(s) 180, 243, 373, 439, 566
BmiI GGNNCC 3 cut(s) 175, 239, 441
BmrFI CCNGG 3 cut(s) 178, 196, 215
BmrI ACTGGG 1 cut(s) 522
BmsI GCATC 1 cut(s) 210
BmuI ACTGGG 1 cut(s) 522
BpmI CTGGAG 1 cut(s) 163
Bpu10I CCTNAGC 1 cut(s) 435
Bpu1102I GCTNAGC 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 500
BsaBI GATNNNNATC 1 cut(s) 385
BsaHI GRCGYC 2 cut(s) 168, 254
BsaI GGTCTC 2 cut(s) 152, 386
BsaJI CCNNGG 4 cut(s) 89, 285, 376, 591
BsaWI WCCGGW 2 cut(s) 234, 240
Bsc4I CCNNNNNNNGG 2 cut(s) 177, 234
Bse118I RCCGGY 4 cut(s) 227, 234, 240, 586
Bse1I ACTGG 1 cut(s) 517
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 385
BseBI CCWGG 3 cut(s) 178, 196, 215
BseDI CCNNGG 4 cut(s) 89, 285, 376, 591
BseGI GGATG 3 cut(s) 65, 78, 201
BseJI GATNNNNATC 1 cut(s) 385
BseLI CCNNNNNNNGG 2 cut(s) 177, 234
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 2 cut(s) 266, 426
BseNI ACTGG 1 cut(s) 517
BseRI GAGGAG 4 cut(s) 14, 17, 20, 32
BseYI CCCAGC 1 cut(s) 451
Bsh1236I CGCG 3 cut(s) 263, 320, 545
Bsh1285I CGRYCG 2 cut(s) 235, 704
BshFI GGCC 5 cut(s) 5, 362, 374, 440, 590
BshNI GGYRCC 1 cut(s) 237
BshTI ACCGGT 2 cut(s) 234, 240
BsiEI CGRYCG 2 cut(s) 235, 704
BsiHKAI GWGCWC 1 cut(s) 656
BsiHKCI CYCGRG 1 cut(s) 655
BsiSI CCGG 5 cut(s) 171, 228, 235, 241, 587
BslI CCNNNNNNNGG 2 cut(s) 177, 234
BsmAI GTCTC 3 cut(s) 152, 335, 386
BsnI GGCC 5 cut(s) 5, 362, 374, 440, 590
Bso31I GGTCTC 2 cut(s) 152, 386
BsoBI CYCGRG 1 cut(s) 655
Bsp1286I GDGCHC 1 cut(s) 656
Bsp143I GATC 5 cut(s) 386, 574, 614, 661, 701
Bsp1720I GCTNAGC 1 cut(s) 275
Bsp19I CCATGG 1 cut(s) 376
Bsp68I TCGCGA 1 cut(s) 263
BspACI CCGC 8 cut(s) 13, 19, 54, 103, 111, 303, 517, 543
BspANI GGCC 5 cut(s) 5, 362, 374, 440, 590
BspCNI CTCAG 2 cut(s) 267, 427
BspFNI CGCG 3 cut(s) 263, 320, 545
BspLI GGNNCC 3 cut(s) 175, 239, 441
BspPI GGATC 1 cut(s) 394
BspT107I GGYRCC 1 cut(s) 237
BspTNI GGTCTC 2 cut(s) 152, 386
BsrDI GCAATG 1 cut(s) 732
BsrFI RCCGGY 4 cut(s) 227, 234, 240, 586
BsrI ACTGG 1 cut(s) 517
BssAI RCCGGY 4 cut(s) 227, 234, 240, 586
BssECI CCNNGG 4 cut(s) 89, 285, 376, 591
BssMI GATC 5 cut(s) 386, 574, 614, 661, 701
BssNI GRCGYC 2 cut(s) 168, 254
BssT1I CCWWGG 3 cut(s) 89, 285, 376
Bst2UI CCWGG 3 cut(s) 178, 196, 215
Bst4CI ACNGT 1 cut(s) 595
Bst6I CTCTTC 1 cut(s) 605
BstACI GRCGYC 2 cut(s) 168, 254
BstDEI CTNAG 2 cut(s) 275, 435
BstDSI CCRYGG 2 cut(s) 376, 591
BstF5I GGATG 3 cut(s) 65, 78, 201
BstFNI CGCG 3 cut(s) 263, 320, 545
BstKTI GATC 5 cut(s) 389, 577, 617, 664, 704
BstMAI GTCTC 3 cut(s) 152, 335, 386
BstMBI GATC 5 cut(s) 386, 574, 614, 661, 701
BstMCI CGRYCG 2 cut(s) 235, 704
BstMWI GCNNNNNNNGC 4 cut(s) 53, 132, 260, 452
BstNI CCWGG 3 cut(s) 178, 196, 215
BstSCI CCNGG 3 cut(s) 176, 194, 213
BstUI CGCG 3 cut(s) 263, 320, 545
BsuRI GGCC 5 cut(s) 5, 362, 374, 440, 590
BtgI CCRYGG 2 cut(s) 376, 591
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 3 cut(s) 65, 78, 201
BtuMI TCGCGA 1 cut(s) 263
Cfr10I RCCGGY 4 cut(s) 227, 234, 240, 586
Cfr13I GGNCC 5 cut(s) 180, 243, 373, 439, 566
CpoI CGGWCCG 1 cut(s) 243
CseI GACGC 3 cut(s) 176, 243, 326
CsiI ACCWGGT 1 cut(s) 176
Csp6I GTAC 1 cut(s) 238
CspAI ACCGGT 2 cut(s) 234, 240
CspI CGGWCCG 1 cut(s) 243
CviAII CATG 2 cut(s) 223, 377
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 2 cut(s) 275, 435
DpnI GATC 5 cut(s) 388, 576, 616, 663, 703
DpnII GATC 5 cut(s) 386, 574, 614, 661, 701
DraI TTTAAA 2 cut(s) 462, 709
DriI GACNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 1 cut(s) 588
Eam1104I CTCTTC 1 cut(s) 605
Eam1105I GACNNNNNGTC 1 cut(s) 253
EarI CTCTTC 1 cut(s) 605
EciI GGCGGA 1 cut(s) 532
Ecl136II GAGCTC 1 cut(s) 654
Eco130I CCWWGG 3 cut(s) 89, 285, 376
Eco24I GRGCYC 1 cut(s) 656
Eco31I GGTCTC 2 cut(s) 152, 386
Eco47I GGWCC 3 cut(s) 180, 243, 566
Eco53kI GAGCTC 1 cut(s) 654
Eco88I CYCGRG 1 cut(s) 655
EcoICRI GAGCTC 1 cut(s) 654
EcoO109I RGGNCCY 2 cut(s) 439, 566
EcoRI GAATTC 1 cut(s) 642
EcoRII CCWGG 3 cut(s) 176, 194, 213
EcoT14I CCWWGG 3 cut(s) 89, 285, 376
EcoT38I GRGCYC 1 cut(s) 656
ErhI CCWWGG 3 cut(s) 89, 285, 376
FaeI CATG 2 cut(s) 226, 380
FaiI YATR 4 cut(s) 224, 378, 559, 561
FatI CATG 2 cut(s) 222, 376
FauI CCCGC 1 cut(s) 61
FbaI TGATCA 1 cut(s) 574
FblI GTMKAC 1 cut(s) 597
Fnu4HI GCNGC 2 cut(s) 111, 304
FokI GGATG 3 cut(s) 52, 65, 188
FriOI GRGCYC 1 cut(s) 656
Fsp4HI GCNGC 2 cut(s) 111, 304
FspBI CTAG 2 cut(s) 90, 739
GluI GCNGC 2 cut(s) 111, 304
GsaI CCCAGC 1 cut(s) 455
GsuI CTGGAG 1 cut(s) 163
HaeIII GGCC 5 cut(s) 5, 362, 374, 440, 590
HapII CCGG 5 cut(s) 171, 228, 235, 241, 587
HgaI GACGC 3 cut(s) 176, 243, 326
Hin1I GRCGYC 2 cut(s) 168, 254
Hin1II CATG 2 cut(s) 226, 380
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HinfI GANTC 4 cut(s) 147, 207, 343, 381
HpaI GTTAAC 1 cut(s) 510
HpaII CCGG 5 cut(s) 171, 228, 235, 241, 587
HphI GGTGA 2 cut(s) 28, 304
Hpy166II GTNNAC 2 cut(s) 510, 598
Hpy188I TCNGA 8 cut(s) 118, 206, 247, 386, 391, 531, 574, 619
Hpy188III TCNNGA 4 cut(s) 157, 262, 496, 657
Hpy8I GTNNAC 2 cut(s) 510, 598
Hpy99I CGWCG 4 cut(s) 122, 170, 263, 341
HpyAV CCTTC 5 cut(s) 307, 406, 563, 579, 626
HpyCH4III ACNGT 1 cut(s) 595
HpyCH4V TGCA 2 cut(s) 201, 563
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 132, 260, 452
HpyF3I CTNAG 2 cut(s) 275, 435
Hsp92I GRCGYC 2 cut(s) 168, 254
Hsp92II CATG 2 cut(s) 226, 380
KpnI GGTACC 1 cut(s) 241
Ksp22I TGATCA 1 cut(s) 574
KspAI GTTAAC 1 cut(s) 510
Kzo9I GATC 5 cut(s) 386, 574, 614, 661, 701
LmnI GCTCC 2 cut(s) 519, 651
LweI GCATC 1 cut(s) 210
MabI ACCWGGT 1 cut(s) 176
MaeI CTAG 2 cut(s) 90, 739
MaeIII GTNAC 1 cut(s) 292
MalI GATC 5 cut(s) 388, 576, 616, 663, 703
MboI GATC 5 cut(s) 386, 574, 614, 661, 701
MboII GAAGA 1 cut(s) 622
MhlI GDGCHC 1 cut(s) 656
MluCI AATT 4 cut(s) 48, 265, 355, 642
MluI ACGCGT 1 cut(s) 318
MlyI GAGTC 1 cut(s) 337
MmeI TCCRAC 3 cut(s) 141, 207, 270
MseI TTAA 3 cut(s) 461, 509, 708
MslI CAYNNNNRTG 1 cut(s) 83
MspA1I CMGCKG 1 cut(s) 141
MspI CCGG 5 cut(s) 171, 228, 235, 241, 587
MspR9I CCNGG 3 cut(s) 178, 196, 215
MssI GTTTAAAC 1 cut(s) 709
MvaI CCWGG 3 cut(s) 178, 196, 215
MvnI CGCG 3 cut(s) 263, 320, 545
MwoI GCNNNNNNNGC 4 cut(s) 53, 132, 260, 452
NcoI CCATGG 1 cut(s) 376
NdeII GATC 5 cut(s) 386, 574, 614, 661, 701
NlaIII CATG 2 cut(s) 226, 380
NlaIV GGNNCC 3 cut(s) 175, 239, 441
NmuCI GTSAC 1 cut(s) 292
NruI TCGCGA 1 cut(s) 263
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 162, 336
PfeI GAWTC 3 cut(s) 147, 207, 381
PfoI TCCNGGA 1 cut(s) 213
PinAI ACCGGT 2 cut(s) 234, 240
PkrI GCNGC 2 cut(s) 112, 305
Ple19I CGATCG 1 cut(s) 704
PleI GAGTC 1 cut(s) 337
PmeI GTTTAAAC 1 cut(s) 709
PpsI GAGTC 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 566
Psp124BI GAGCTC 1 cut(s) 656
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 3 cut(s) 176, 194, 213
PspFI CCCAGC 1 cut(s) 451
PspGI CCWGG 3 cut(s) 176, 194, 213
PspN4I GGNNCC 3 cut(s) 175, 239, 441
PspPI GGNCC 5 cut(s) 180, 243, 373, 439, 566
PspPPI RGGWCCY 1 cut(s) 566
PvuI CGATCG 1 cut(s) 704
PvuII CAGCTG 1 cut(s) 141
RruI TCGCGA 1 cut(s) 263
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 83
Rsr2I CGGWCCG 1 cut(s) 243
RsrII CGGWCCG 1 cut(s) 243
SacI GAGCTC 1 cut(s) 656
SaqAI TTAA 3 cut(s) 461, 509, 708
SatI GCNGC 2 cut(s) 111, 304
Sau3AI GATC 5 cut(s) 386, 574, 614, 661, 701
Sau96I GGNCC 5 cut(s) 180, 243, 373, 439, 566
SchI GAGTC 1 cut(s) 337
ScrFI CCNGG 3 cut(s) 178, 196, 215
SduI GDGCHC 1 cut(s) 656
SexAI ACCWGGT 1 cut(s) 176
SfaNI GCATC 1 cut(s) 210
SinI GGWCC 3 cut(s) 180, 243, 566
SmiMI CAYNNNNRTG 1 cut(s) 83
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 4 cut(s) 48, 265, 355, 642
SsiI CCGC 8 cut(s) 13, 19, 54, 103, 111, 303, 517, 543
SspMI CTAG 2 cut(s) 90, 739
SstI GAGCTC 1 cut(s) 656
StyD4I CCNGG 3 cut(s) 176, 194, 213
StyI CCWWGG 3 cut(s) 89, 285, 376
TaaI ACNGT 1 cut(s) 595
TaqI TCGA 5 cut(s) 156, 330, 336, 414, 640
TasI AATT 4 cut(s) 48, 265, 355, 642
TauI GCSGC 2 cut(s) 113, 306
TfiI GAWTC 3 cut(s) 147, 207, 381
Tru1I TTAA 3 cut(s) 461, 509, 708
Tru9I TTAA 3 cut(s) 461, 509, 708
TseFI GTSAC 1 cut(s) 292
Tsp45I GTSAC 1 cut(s) 292
TspDTI ATGAA 2 cut(s) 417, 645
TspGWI ACGGA 3 cut(s) 64, 180, 247
VpaK11BI GGWCC 3 cut(s) 180, 243, 566
XapI RAATTY 1 cut(s) 642
XmaJI CCTAGG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 597
XspI CTAG 2 cut(s) 90, 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.