Rroxscaffold_2G00149330

acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012044
Physical Location & Seq
Reverse (-)
86923526 .. 86926002
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149330.1

Sequence Viewer

Length: 783 bp
ATGGCCTCCTCCTCCCTCCGCCTCCTCCTCATCGTCCTCGTCACCATCCCCGCCGCCCTGTCCCACACCATCCTCCAAATCCCGCCGAACAGACACGCCTCCTCCGAGGACCGCAAGCTCCGCGCCGACGACAACCTCTTCTGCGACAGCTGGCGGTTCTCCGTCGAGACCAACGACGCCGGAACCTGGGACAGCATTCCCTCCAGGTGCGTCGGATTCGTCCAGGACTACATGATCGGCTCCCGGTACGCCTCCGACTCGGCTGCCGTTGCGAATTTCTCGCTGGCGTTCGGTCAGGCGGTGAAGCTCGGCGGCGACGGCAAGGACGCCTGGGTGTTCGATATCGATGAGACTCTGGTGTCCAACTTGCCGTACTACGAGGCCCATGGGTTCGGATCAGTGACATTCGATGAAGAAGCTTTTGATGAGTGGGTGGATTTGGCTGAGGCCCCTGCTATCCCAGCAAGTTTGAATCTGTACAAGGGGCTCCAACGGTTGGGTTTCAAGATTTTCCTGTTGACTGGGCGGAGTGAATTTCAGAGAAATGCCACGGTGAAAAACCTCCTATATTCAGGTTACAGTGACTGGGAGAGGCTGCTACTGAGGGGACCTTCAGATAAAGGAACACTCGCGACTGAATACAAATCCAAGAAGAGGGCAGAACTGATAACCGAAGGTTATCGACTTCATGGGAGCTCGGGAGATCAATGGAGTGACTTGTCGGGCTTTGCAGTTGCCCGACGATCCTTTAAACTTCCAAACCCAATGTATTATATTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

260

Amino Acids

28.93

Weight (kDa)

5.45

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 42 - 259 9.6e-77 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29260 AT4G29270
fragaria_vesca FvH4_1g06500
malus_domestica MD02G1068300.v1.1
prunus_persica Prupe.7G217300_v2.0.a1
pyrus_communis pycom02g05410 pycom08g17150 pycom15g17740
rosa_chinensis RchiOBHm_Chr2g0092251
rosa_laevigata RLG00000016290
rosa_multiflora Rmu_sc0000524.1_g000032 Rmu_sc0030540.1_g000001
rosa_roxburghii Rroxscaffold_2G00149330
rosa_rugosa Rorug02G0024100
rosa_samantha Rh2AG069900 Rh2BG069400 Rh2CG070600 Rh2DG068400
rosa_wichuraiana Rw2G006230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 358
AccB7I CCANNNNNTGG 1 cut(s) 496
AccII CGCG 2 cut(s) 123, 632
AclWI GGATC 2 cut(s) 403, 738
AcsI RAATTY 2 cut(s) 274, 533
AcuI CTGAAG 1 cut(s) 597
AcyI GRCGYC 2 cut(s) 177, 327
AfaI GTAC 3 cut(s) 248, 374, 479
AfiI CCNNNNNNNGG 3 cut(s) 186, 496, 654
AgsI TTSAA 2 cut(s) 472, 505
AjnI CCWGG 4 cut(s) 185, 203, 222, 329
AluBI AGCT 5 cut(s) 118, 150, 307, 419, 696
AluI AGCT 5 cut(s) 118, 150, 307, 419, 696
Alw21I GWGCWC 1 cut(s) 698
Alw26I GTCTC 2 cut(s) 161, 344
AlwI GGATC 2 cut(s) 403, 738
AlwNI CAGNNNCTG 1 cut(s) 585
Ama87I CYCGRG 1 cut(s) 697
AoxI GGCC 3 cut(s) 3, 381, 447
ApeKI GCWGC 2 cut(s) 263, 595
ApoI RAATTY 2 cut(s) 274, 533
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 4 cut(s) 109, 382, 448, 608
AsuC2I CCSGG 1 cut(s) 244
AsuHPI GGTGA 3 cut(s) 34, 313, 565
AvaI CYCGRG 1 cut(s) 697
AvaII GGWCC 2 cut(s) 109, 608
BanII GRGCYC 2 cut(s) 489, 698
Bbv12I GWGCWC 1 cut(s) 698
BbvCI CCTCAGC 1 cut(s) 444
BbvI GCAGC 2 cut(s) 250, 582
BccI CCATC 2 cut(s) 53, 77
BceAI ACGGC 3 cut(s) 251, 334, 355
BcgI CGANNNNNNTGC 2 cut(s) 245, 279
BciT130I CCWGG 4 cut(s) 187, 205, 224, 331
BcnI CCSGG 1 cut(s) 244
BcoDI GTCTC 2 cut(s) 161, 344
BisI GCNGC 4 cut(s) 54, 264, 313, 596
BlsI GCNGC 4 cut(s) 55, 265, 314, 597
Bme1390I CCNGG 5 cut(s) 187, 205, 224, 244, 331
Bme18I GGWCC 2 cut(s) 109, 608
BmeT110I CYCGRG 1 cut(s) 697
BmgT120I GGNCC 4 cut(s) 109, 382, 448, 608
BmiI GGNNCC 5 cut(s) 184, 241, 450, 488, 609
BmrFI CCNGG 5 cut(s) 187, 205, 224, 244, 331
BmrI ACTGGG 2 cut(s) 531, 595
BmuI ACTGGG 2 cut(s) 531, 595
BpmI CTGGAG 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 244
Bsa29I ATCGAT 1 cut(s) 345
BsaHI GRCGYC 2 cut(s) 177, 327
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 5 cut(s) 105, 186, 330, 385, 549
BsaXI ACNNNNNCTCC 2 cut(s) 86, 116
Bsc4I CCNNNNNNNGG 3 cut(s) 186, 496, 654
Bse1I ACTGG 2 cut(s) 526, 590
BseBI CCWGG 4 cut(s) 187, 205, 224, 331
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 5 cut(s) 105, 186, 330, 385, 549
BseGI GGATG 2 cut(s) 45, 69
BseLI CCNNNNNNNGG 3 cut(s) 186, 496, 654
BseMII CTCAG 2 cut(s) 435, 593
BseNI ACTGG 2 cut(s) 526, 590
BseRI GAGGAG 3 cut(s) 14, 17, 91
BseXI GCAGC 2 cut(s) 250, 582
BseYI CCCAGC 1 cut(s) 460
Bsh1236I CGCG 2 cut(s) 123, 632
BshFI GGCC 3 cut(s) 5, 383, 449
BshVI ATCGAT 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 698
BsiHKCI CYCGRG 1 cut(s) 697
BsiSI CCGG 2 cut(s) 180, 244
BslFI GGGAC 3 cut(s) 46, 203, 621
BslI CCNNNNNNNGG 3 cut(s) 186, 496, 654
BsmAI GTCTC 2 cut(s) 161, 344
BsmFI GGGAC 3 cut(s) 46, 203, 621
BsmI GAATGC 1 cut(s) 195
BsnI GGCC 3 cut(s) 5, 383, 449
Bso31I GGTCTC 1 cut(s) 161
BsoBI CYCGRG 1 cut(s) 697
Bsp1286I GDGCHC 2 cut(s) 489, 698
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 4 cut(s) 234, 395, 703, 743
Bsp19I CCATGG 1 cut(s) 385
Bsp68I TCGCGA 1 cut(s) 632
BspANI GGCC 3 cut(s) 5, 383, 449
BspCNI CTCAG 2 cut(s) 436, 594
BspDI ATCGAT 1 cut(s) 345
BspFNI CGCG 2 cut(s) 123, 632
BspLI GGNNCC 5 cut(s) 184, 241, 450, 488, 609
BspPI GGATC 2 cut(s) 403, 738
BspTNI GGTCTC 1 cut(s) 161
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 2 cut(s) 526, 590
BssECI CCNNGG 5 cut(s) 105, 186, 330, 385, 549
BssMI GATC 4 cut(s) 234, 395, 703, 743
BssNI GRCGYC 2 cut(s) 177, 327
BssT1I CCWWGG 1 cut(s) 385
Bst2UI CCWGG 4 cut(s) 187, 205, 224, 331
Bst4CI ACNGT 3 cut(s) 495, 553, 581
Bst6I CTCTTC 2 cut(s) 143, 647
BstACI GRCGYC 2 cut(s) 177, 327
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 3 cut(s) 116, 152, 285
BstDEI CTNAG 2 cut(s) 444, 602
BstDSI CCRYGG 2 cut(s) 385, 549
BstF5I GGATG 2 cut(s) 45, 69
BstFNI CGCG 2 cut(s) 123, 632
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 4 cut(s) 237, 398, 706, 746
BstMAI GTCTC 2 cut(s) 161, 344
BstMBI GATC 4 cut(s) 234, 395, 703, 743
BstMWI GCNNNNNNNGC 4 cut(s) 120, 269, 318, 461
BstNI CCWGG 4 cut(s) 187, 205, 224, 331
BstSCI CCNGG 5 cut(s) 185, 203, 222, 242, 329
BstUI CGCG 2 cut(s) 123, 632
BstV1I GCAGC 2 cut(s) 250, 582
Bsu15I ATCGAT 1 cut(s) 345
BsuRI GGCC 3 cut(s) 5, 383, 449
BsuTUI ATCGAT 1 cut(s) 345
BtgI CCRYGG 2 cut(s) 385, 549
BtsCI GGATG 2 cut(s) 45, 69
BtsIMutI CAGTG 2 cut(s) 405, 586
BtuMI TCGCGA 1 cut(s) 632
Cac8I GCNNGC 3 cut(s) 116, 152, 285
CaiI CAGNNNCTG 1 cut(s) 585
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 4 cut(s) 109, 382, 448, 608
ClaI ATCGAT 1 cut(s) 345
CseI GACGC 3 cut(s) 185, 199, 335
Csp6I GTAC 3 cut(s) 247, 373, 478
CviAII CATG 3 cut(s) 232, 386, 689
CviQI GTAC 3 cut(s) 247, 373, 478
DdeI CTNAG 2 cut(s) 444, 602
DpnI GATC 4 cut(s) 236, 397, 705, 745
DpnII GATC 4 cut(s) 234, 395, 703, 743
DraI TTTAAA 1 cut(s) 751
DrdI GACNNNNNNGTC 1 cut(s) 358
DseDI GACNNNNNNGTC 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 143, 647
EarI CTCTTC 2 cut(s) 143, 647
EciI GGCGGA 2 cut(s) 8, 541
Ecl136II GAGCTC 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 2 cut(s) 489, 698
Eco31I GGTCTC 1 cut(s) 161
Eco32I GATATC 1 cut(s) 343
Eco47I GGWCC 2 cut(s) 109, 608
Eco53kI GAGCTC 1 cut(s) 696
Eco57I CTGAAG 1 cut(s) 597
Eco88I CYCGRG 1 cut(s) 697
EcoICRI GAGCTC 1 cut(s) 696
EcoO109I RGGNCCY 2 cut(s) 448, 608
EcoRII CCWGG 4 cut(s) 185, 203, 222, 329
EcoRV GATATC 1 cut(s) 343
EcoT14I CCWWGG 1 cut(s) 385
EcoT38I GRGCYC 2 cut(s) 489, 698
ErhI CCWWGG 1 cut(s) 385
FaeI CATG 3 cut(s) 235, 389, 692
FaiI YATR 6 cut(s) 233, 387, 568, 690, 774, 781
FaqI GGGAC 3 cut(s) 46, 203, 621
FatI CATG 3 cut(s) 231, 385, 688
FauI CCCGC 2 cut(s) 58, 90
Fnu4HI GCNGC 4 cut(s) 54, 264, 313, 596
FokI GGATG 2 cut(s) 32, 56
FriOI GRGCYC 2 cut(s) 489, 698
Fsp4HI GCNGC 4 cut(s) 54, 264, 313, 596
GlaI GCGC 1 cut(s) 124
GluI GCNGC 4 cut(s) 54, 264, 313, 596
GsaI CCCAGC 1 cut(s) 464
GsuI CTGGAG 1 cut(s) 187
HaeIII GGCC 3 cut(s) 5, 383, 449
HapII CCGG 2 cut(s) 180, 244
HgaI GACGC 3 cut(s) 185, 199, 335
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 2 cut(s) 177, 327
Hin1II CATG 3 cut(s) 235, 389, 692
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 519
HindII GTYRAC 1 cut(s) 519
HindIII AAGCTT 1 cut(s) 417
HinfI GANTC 4 cut(s) 216, 257, 352, 472
HpaII CCGG 2 cut(s) 180, 244
HphI GGTGA 3 cut(s) 34, 313, 565
Hpy166II GTNNAC 1 cut(s) 519
Hpy188I TCNGA 6 cut(s) 106, 215, 256, 395, 540, 616
Hpy188III TCNNGA 4 cut(s) 166, 505, 631, 699
Hpy8I GTNNAC 1 cut(s) 519
Hpy99I CGWCG 6 cut(s) 131, 167, 179, 215, 320, 744
HpyAV CCTTC 2 cut(s) 621, 668
HpyCH4III ACNGT 3 cut(s) 495, 553, 581
HpyCH4V TGCA 2 cut(s) 731, 779
HpyF10VI GCNNNNNNNGC 4 cut(s) 120, 269, 318, 461
HpyF3I CTNAG 2 cut(s) 444, 602
Hsp92I GRCGYC 2 cut(s) 177, 327
Hsp92II CATG 3 cut(s) 235, 389, 692
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 4 cut(s) 234, 395, 703, 743
LmnI GCTCC 4 cut(s) 123, 245, 492, 693
Lsp1109I GCAGC 2 cut(s) 250, 582
MaeIII GTNAC 5 cut(s) 40, 400, 575, 581, 713
MalI GATC 4 cut(s) 236, 397, 705, 745
MboI GATC 4 cut(s) 234, 395, 703, 743
MboII GAAGA 3 cut(s) 130, 425, 664
MhlI GDGCHC 2 cut(s) 489, 698
MluCI AATT 2 cut(s) 274, 533
MlyI GAGTC 2 cut(s) 251, 346
MmeI TCCRAC 4 cut(s) 193, 279, 387, 514
MseI TTAA 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 150
MspI CCGG 2 cut(s) 180, 244
MspR9I CCNGG 5 cut(s) 187, 205, 224, 244, 331
Mva1269I GAATGC 1 cut(s) 195
MvaI CCWGG 4 cut(s) 187, 205, 224, 331
MvnI CGCG 2 cut(s) 123, 632
MwoI GCNNNNNNNGC 4 cut(s) 120, 269, 318, 461
NciI CCSGG 1 cut(s) 244
NcoI CCATGG 1 cut(s) 385
NdeII GATC 4 cut(s) 234, 395, 703, 743
NlaIII CATG 3 cut(s) 235, 389, 692
NlaIV GGNNCC 5 cut(s) 184, 241, 450, 488, 609
NmeAIII GCCGAG 2 cut(s) 239, 288
NmuCI GTSAC 4 cut(s) 40, 400, 581, 713
NruI TCGCGA 1 cut(s) 632
PcsI WCGNNNNNNNCGW 2 cut(s) 102, 171
PctI GAATGC 1 cut(s) 195
PfeI GAWTC 2 cut(s) 216, 472
PflMI CCANNNNNTGG 1 cut(s) 496
PfoI TCCNGGA 1 cut(s) 222
PkrI GCNGC 4 cut(s) 55, 265, 314, 597
PleI GAGTC 2 cut(s) 251, 346
PpsI GAGTC 2 cut(s) 251, 346
PpuMI RGGWCCY 1 cut(s) 608
Psp124BI GAGCTC 1 cut(s) 698
Psp5II RGGWCCY 1 cut(s) 608
Psp6I CCWGG 4 cut(s) 185, 203, 222, 329
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 4 cut(s) 185, 203, 222, 329
PspN4I GGNNCC 5 cut(s) 184, 241, 450, 488, 609
PspPI GGNCC 4 cut(s) 109, 382, 448, 608
PspPPI RGGWCCY 1 cut(s) 608
PstNI CAGNNNCTG 1 cut(s) 585
PvuII CAGCTG 1 cut(s) 150
RruI TCGCGA 1 cut(s) 632
RsaI GTAC 3 cut(s) 248, 374, 479
RsaNI GTAC 3 cut(s) 247, 373, 478
SacI GAGCTC 1 cut(s) 698
SaqAI TTAA 1 cut(s) 750
SatI GCNGC 4 cut(s) 54, 264, 313, 596
Sau3AI GATC 4 cut(s) 234, 395, 703, 743
Sau96I GGNCC 4 cut(s) 109, 382, 448, 608
SchI GAGTC 2 cut(s) 251, 346
ScrFI CCNGG 5 cut(s) 187, 205, 224, 244, 331
SduI GDGCHC 2 cut(s) 489, 698
SinI GGWCC 2 cut(s) 109, 608
Sse9I AATT 2 cut(s) 274, 533
SstI GAGCTC 1 cut(s) 698
StyD4I CCNGG 5 cut(s) 185, 203, 222, 242, 329
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 3 cut(s) 495, 553, 581
TaqI TCGA 5 cut(s) 165, 339, 345, 408, 682
TaqII GACCGA 1 cut(s) 281
TasI AATT 2 cut(s) 274, 533
TatI WGTACW 1 cut(s) 477
TauI GCSGC 2 cut(s) 56, 315
TfiI GAWTC 2 cut(s) 216, 472
Tru1I TTAA 1 cut(s) 750
Tru9I TTAA 1 cut(s) 750
TscAI CASTG 2 cut(s) 405, 586
TseFI GTSAC 4 cut(s) 40, 400, 581, 713
TseI GCWGC 2 cut(s) 263, 595
Tsp45I GTSAC 4 cut(s) 40, 400, 581, 713
TspDTI ATGAA 2 cut(s) 426, 677
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 2 cut(s) 405, 586
Van91I CCANNNNNTGG 1 cut(s) 496
VpaK11BI GGWCC 2 cut(s) 109, 608
XapI RAATTY 2 cut(s) 274, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.