FvH4_1g06530

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007454
Physical Location & Seq
Reverse (-)
3441535 .. 3446875
5341 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06530.t1

Sequence Viewer

Length: 1497 bp
ATGAATAAGAGTAATATCAGAAGCTCTGATCTCATTGATGCCAAGCTTGAAGAACACCAGATGTGTGGATCGAAGCAGTGCCCCGGTTGCGGACACAAACTTGAAGGAAAGCCGGACTGGTTAGGTCTACCAGCAGGAGTGAAATTTGACCCAACAGACCAAGAACTGATTGAACACCTTGAAGCAAAGGTAGAAGCCAAAGACACAAAGTCTCATCCTTTGATTGATGAGTTCATTCCTACCATTGAAGGAGAAGATGGCATTTGTTACACCCATCCTGAAAAACTTCCTGGAGTGACTAGAGATGGTTTGAGCAGACACTTCTTCCATAGACCCTCCAAGGCTTACACAACTGGGACACGAAAGAGGCGAAAAATCCAGACAGAATGCGACCACTTGCAAGCAGGCGGGGAGACAAGGTGGCACAAAACCGGCAAGACCAGACCGGTAATGGTGAATGGCAAGCAAAAAGGGTGCAAGAAAATCCTGGTCCTCTACACAAACTTCGGGAAGAATCGAAAACCGGAGAAGACGAACTGGGTGATGCACCAGTACCATCTAGGCCAGCACGAGGAGGAGAAGGAAGGAGAGCTCGTGGTTTCGAAGATATTCTATCAGACGCAGCCGAGACAATGCAACTGGTCGTCCGAGAGATCAAGTGCTGCAAGTGCAAGTTTAACCGGCGAAGGAAGTAGTGATTTGAGAAGAGACAGTGGTGGTAGTGGGAGTTGTACTTCTTCGAAGGAAATAATAGTGTCCCATAATACTCATCTTCGAGATCACCATGATCAAATGTCCGCAGCAGCAGCAGCTCAAGTTAATGTCGTTGGTCAGCCATTAACAAGTTACAATGCCATAGACATTCATCAGTTGAAGTCCGACCATTTCAGCTTTGTCCCATTCAGAAAAAGCTTCGATGAGGTGGGGATAGGAGAGGCTTCAACGGCAAGGGAACCCAGCATTGCATCTGGCACGTGTGAAGATCTGCGTGAGAGACCACAGCTTCATCATGTTGCACATGATCAACTGCTTCATCACCACCAACATCAACAGCAGGTACACCATGAGCAACAGCAACATCAGCATGGCCAAACACATCTTCAACAACAAATAGCAACGGCGGCCAGCTTCCACATCAGTCGGCCTTCACACCCCATTTCCACCATCATCTCTCCGCCTTCTCTTCATCACTCCGCCACCTCCATCATGCTCGATCAGGATGCTTACCATGTCTCCCGATCGATAATGCTCCAAAACGAACATTTCCAGCAACAGCAACAACAGCAGCAGCAGCAACAACAACAACAACAACAGCAGCAGCATCATAAACAGATGGGAGGGAGGTCAGCATCTGGCTTAGAGGAGCTCATAATGGCCTGCACTTCATCGAGTAATGTCAAAGAAGAGTCATCATCCATGGCAAACCCTCAAGAGGCAGAGTGGATGAAGTACTCTCCATTCTGGCCTGACCCTGACAACACAGATCATCATGGATAG

Protein Analysis

499

Amino Acids

56.2

Weight (kDa)

6.65

Isoelectric Point (pI)

59.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 44 - 187 1.5e-25 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1045
AccI GTMKAC 1 cut(s) 127
AciI CCGC 6 cut(s) 90, 408, 798, 1121, 1175, 1194
AclWI GGATC 1 cut(s) 76
AcoI YGGCCR 2 cut(s) 1087, 1122
AcsI RAATTY 1 cut(s) 143
AcvI CACGTG 1 cut(s) 975
AfaI GTAC 4 cut(s) 554, 733, 1059, 1451
AfiI CCNNNNNNNGG 3 cut(s) 89, 571, 1432
AflIII ACRYGT 1 cut(s) 974
AgeI ACCGGT 1 cut(s) 445
AgsI TTSAA 8 cut(s) 50, 104, 173, 182, 248, 874, 942, 1103
AhdI GACNNNNNGTC 1 cut(s) 208
AjnI CCWGG 2 cut(s) 289, 486
AjuI GAANNNNNNNTTGG 2 cut(s) 1083, 1115
AluBI AGCT 9 cut(s) 24, 46, 592, 812, 891, 912, 1003, 1128, 1366
AluI AGCT 9 cut(s) 24, 46, 592, 812, 891, 912, 1003, 1128, 1366
Alw21I GWGCWC 2 cut(s) 594, 1368
Alw26I GTCTC 6 cut(s) 216, 407, 622, 702, 988, 1237
AlwI GGATC 1 cut(s) 76
AlwNI CAGNNNCTG 1 cut(s) 1352
AoxI GGCC 6 cut(s) 562, 1087, 1122, 1142, 1374, 1463
ApoI RAATTY 1 cut(s) 143
AsiGI ACCGGT 1 cut(s) 445
Asp700I GAANNNNTTC 2 cut(s) 285, 608
AspS9I GGNCC 1 cut(s) 490
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 466, 553, 773, 1028
AsuII TTCGAA 2 cut(s) 602, 740
AvaII GGWCC 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 83
BalI TGGCCA 1 cut(s) 1089
BanII GRGCYC 2 cut(s) 594, 1368
BauI CACGAG 2 cut(s) 569, 593
BbrPI CACGTG 1 cut(s) 975
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 594, 1368
BccI CCATC 7 cut(s) 251, 282, 299, 564, 1172, 1211, 1327
BceAI ACGGC 2 cut(s) 960, 1134
BcgI CGANNNNNNTGC 2 cut(s) 1202, 1236
BciT130I CCWGG 2 cut(s) 291, 488
BclI TGATCA 2 cut(s) 787, 1021
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 6 cut(s) 216, 407, 622, 702, 988, 1237
BfaI CTAG 2 cut(s) 300, 560
BfuAI ACCTGC 1 cut(s) 1045
BglII AGATCT 1 cut(s) 982
BmcAI AGTACT 1 cut(s) 1451
Bme1390I CCNGG 3 cut(s) 84, 291, 488
Bme18I GGWCC 1 cut(s) 490
BmeRI GACNNNNNGTC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 490
BmiI GGNNCC 1 cut(s) 954
BmrFI CCNGG 3 cut(s) 84, 291, 488
BmrI ACTGGG 2 cut(s) 363, 547
BmsI GCATC 6 cut(s) 28, 534, 974, 1210, 1330, 1358
BmuI ACTGGG 2 cut(s) 363, 547
BpiI GAAGAC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 312
Bpu14I TTCGAA 2 cut(s) 602, 740
BpuEI CTTGAG 2 cut(s) 798, 1413
BpuMI CCSGG 1 cut(s) 84
Bsa29I ATCGAT 1 cut(s) 1241
BsaAI YACGTR 1 cut(s) 975
BsaI GGTCTC 1 cut(s) 988
BsaJI CCNNGG 3 cut(s) 82, 339, 1416
BsaWI WCCGGW 2 cut(s) 445, 523
BsaXI ACNNNNNCTCC 6 cut(s) 404, 434, 579, 609, 1217, 1247
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 571, 1432
Bse118I RCCGGY 3 cut(s) 431, 445, 680
Bse1I ACTGG 5 cut(s) 122, 358, 542, 550, 644
Bse3DI GCAATG 1 cut(s) 960
BseBI CCWGG 2 cut(s) 291, 488
BseCI ATCGAT 1 cut(s) 1241
BseDI CCNNGG 3 cut(s) 82, 339, 1416
BseGI GGATG 5 cut(s) 214, 274, 1225, 1412, 1449
BseLI CCNNNNNNNGG 3 cut(s) 89, 571, 1432
BseMI GCAATG 1 cut(s) 960
BseNI ACTGG 5 cut(s) 122, 358, 542, 550, 644
BseRI GAGGAG 3 cut(s) 587, 590, 1376
BseSI GKGCMC 1 cut(s) 83
BseYI CCCAGC 1 cut(s) 956
BsgI GTGCAG 1 cut(s) 1363
Bsh1285I CGRYCG 1 cut(s) 1241
BshFI GGCC 6 cut(s) 564, 1089, 1124, 1144, 1376, 1465
BshTI ACCGGT 1 cut(s) 445
BshVI ATCGAT 1 cut(s) 1241
BsiEI CGRYCG 1 cut(s) 1241
BsiHKAI GWGCWC 2 cut(s) 594, 1368
BsiSI CCGG 6 cut(s) 84, 113, 432, 446, 524, 681
BslFI GGGAC 3 cut(s) 370, 742, 881
BslI CCNNNNNNNGG 3 cut(s) 89, 571, 1432
BsmAI GTCTC 6 cut(s) 216, 407, 622, 702, 988, 1237
BsmFI GGGAC 3 cut(s) 370, 742, 881
BsmI GAATGC 1 cut(s) 392
BsnI GGCC 6 cut(s) 564, 1089, 1124, 1144, 1376, 1465
Bso31I GGTCTC 1 cut(s) 988
Bsp119I TTCGAA 2 cut(s) 602, 740
Bsp1286I GDGCHC 3 cut(s) 83, 594, 1368
Bsp19I CCATGG 1 cut(s) 1416
BspACI CCGC 6 cut(s) 90, 408, 798, 1121, 1175, 1194
BspANI GGCC 6 cut(s) 564, 1089, 1124, 1144, 1376, 1465
BspDI ATCGAT 1 cut(s) 1241
BspLI GGNNCC 1 cut(s) 954
BspMI ACCTGC 1 cut(s) 1045
BspPI GGATC 1 cut(s) 76
BspT104I TTCGAA 2 cut(s) 602, 740
BspTNI GGTCTC 1 cut(s) 988
BsrDI GCAATG 1 cut(s) 960
BsrFI RCCGGY 3 cut(s) 431, 445, 680
BsrI ACTGG 5 cut(s) 122, 358, 542, 550, 644
BssAI RCCGGY 3 cut(s) 431, 445, 680
BssECI CCNNGG 3 cut(s) 82, 339, 1416
BssSI CACGAG 2 cut(s) 569, 593
BssT1I CCWWGG 2 cut(s) 339, 1416
Bst2BI CACGAG 2 cut(s) 569, 593
Bst2UI CCWGG 2 cut(s) 291, 488
Bst4CI ACNGT 1 cut(s) 713
Bst6I CTCTTC 3 cut(s) 700, 1188, 1398
BstBAI YACGTR 1 cut(s) 975
BstBI TTCGAA 2 cut(s) 602, 740
BstC8I GCNNGC 6 cut(s) 402, 406, 464, 566, 1126, 1378
BstDEI CTNAG 1 cut(s) 1357
BstDSI CCRYGG 1 cut(s) 1416
BstF5I GGATG 5 cut(s) 214, 274, 1225, 1412, 1449
BstMAI GTCTC 6 cut(s) 216, 407, 622, 702, 988, 1237
BstMCI CGRYCG 1 cut(s) 1241
BstMWI GCNNNNNNNGC 9 cut(s) 87, 668, 806, 809, 944, 1081, 1121, 1282, 1291
BstNI CCWGG 2 cut(s) 291, 488
BstSCI CCNGG 3 cut(s) 82, 289, 486
BstSLI GKGCMC 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 536
BstX2I RGATCY 1 cut(s) 982
BstXI CCANNNNNNTGG 1 cut(s) 65
BstYI RGATCY 1 cut(s) 982
Bsu15I ATCGAT 1 cut(s) 1241
BsuRI GGCC 6 cut(s) 564, 1089, 1124, 1144, 1376, 1465
BsuTUI ATCGAT 1 cut(s) 1241
BtgI CCRYGG 1 cut(s) 1416
BtsCI GGATG 5 cut(s) 214, 274, 1225, 1412, 1449
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 2 cut(s) 83, 718
BveI ACCTGC 1 cut(s) 1045
Cac8I GCNNGC 6 cut(s) 402, 406, 464, 566, 1126, 1378
CaiI CAGNNNCTG 1 cut(s) 1352
Cfr10I RCCGGY 3 cut(s) 431, 445, 680
Cfr13I GGNCC 1 cut(s) 490
ClaI ATCGAT 1 cut(s) 1241
CseI GACGC 1 cut(s) 628
Csp6I GTAC 4 cut(s) 553, 732, 1058, 1450
CspAI ACCGGT 1 cut(s) 445
CviAII CATG 9 cut(s) 785, 1010, 1019, 1064, 1085, 1207, 1229, 1417, 1490
CviQI GTAC 4 cut(s) 553, 732, 1058, 1450
DdeI CTNAG 1 cut(s) 1357
DriI GACNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 2 cut(s) 1087, 1122
Eam1104I CTCTTC 3 cut(s) 700, 1188, 1398
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 3 cut(s) 700, 1188, 1398
EciI GGCGGA 2 cut(s) 1164, 1183
Ecl136II GAGCTC 2 cut(s) 592, 1366
Eco130I CCWWGG 2 cut(s) 339, 1416
Eco24I GRGCYC 2 cut(s) 594, 1368
Eco31I GGTCTC 1 cut(s) 988
Eco47I GGWCC 1 cut(s) 490
Eco53kI GAGCTC 2 cut(s) 592, 1366
Eco72I CACGTG 1 cut(s) 975
EcoICRI GAGCTC 2 cut(s) 592, 1366
EcoRII CCWGG 2 cut(s) 289, 486
EcoT14I CCWWGG 2 cut(s) 339, 1416
EcoT38I GRGCYC 2 cut(s) 594, 1368
ErhI CCWWGG 2 cut(s) 339, 1416
FaeI CATG 9 cut(s) 788, 1013, 1022, 1067, 1088, 1210, 1232, 1420, 1493
FaqI GGGAC 3 cut(s) 370, 742, 881
FatI CATG 9 cut(s) 784, 1009, 1018, 1063, 1084, 1206, 1228, 1416, 1489
FauI CCCGC 1 cut(s) 401
FbaI TGATCA 2 cut(s) 787, 1021
FblI GTMKAC 1 cut(s) 127
FokI GGATG 5 cut(s) 201, 261, 1232, 1399, 1456
FriOI GRGCYC 2 cut(s) 594, 1368
FspBI CTAG 2 cut(s) 300, 560
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 6 cut(s) 564, 1089, 1124, 1144, 1376, 1465
HapII CCGG 6 cut(s) 84, 113, 432, 446, 524, 681
HgaI GACGC 1 cut(s) 628
Hin1II CATG 9 cut(s) 788, 1013, 1022, 1067, 1088, 1210, 1232, 1420, 1493
HindIII AAGCTT 2 cut(s) 44, 910
HinfI GANTC 2 cut(s) 514, 1406
HpaII CCGG 6 cut(s) 84, 113, 432, 446, 524, 681
HphI GGTGA 4 cut(s) 466, 553, 773, 1028
Hpy166II GTNNAC 2 cut(s) 128, 1060
Hpy188I TCNGA 6 cut(s) 20, 28, 618, 649, 880, 905
Hpy188III TCNNGA 7 cut(s) 278, 379, 508, 776, 1217, 1236, 1430
Hpy8I GTNNAC 2 cut(s) 128, 1060
HpyAV CCTTC 8 cut(s) 98, 242, 574, 578, 680, 736, 1155, 1188
HpyCH4III ACNGT 1 cut(s) 713
HpyCH4IV ACGT 1 cut(s) 974
HpyCH4V TGCA 9 cut(s) 400, 477, 547, 636, 665, 671, 965, 1016, 1380
HpyF10VI GCNNNNNNNGC 9 cut(s) 87, 668, 806, 809, 944, 1081, 1121, 1282, 1291
HpyF3I CTNAG 1 cut(s) 1357
HpySE526I ACGT 1 cut(s) 974
Hsp92II CATG 9 cut(s) 788, 1013, 1022, 1067, 1088, 1210, 1232, 1420, 1493
Ksp22I TGATCA 2 cut(s) 787, 1021
LmnI GCTCC 2 cut(s) 1254, 1363
LweI GCATC 6 cut(s) 28, 534, 974, 1210, 1330, 1358
MaeI CTAG 2 cut(s) 300, 560
MaeII ACGT 1 cut(s) 974
MaeIII GTNAC 3 cut(s) 266, 295, 845
MflI RGATCY 1 cut(s) 982
MhlI GDGCHC 3 cut(s) 83, 594, 1368
MlsI TGGCCA 1 cut(s) 1089
MluCI AATT 1 cut(s) 143
MluNI TGGCCA 1 cut(s) 1089
MlyI GAGTC 1 cut(s) 1415
MmeI TCCRAC 1 cut(s) 903
Mox20I TGGCCA 1 cut(s) 1089
MroXI GAANNNNTTC 2 cut(s) 285, 608
MscI TGGCCA 1 cut(s) 1089
MseI TTAA 3 cut(s) 677, 819, 839
MslI CAYNNNNRTG 1 cut(s) 1083
Msp20I TGGCCA 1 cut(s) 1089
MspI CCGG 6 cut(s) 84, 113, 432, 446, 524, 681
MspR9I CCNGG 3 cut(s) 84, 291, 488
Mva1269I GAATGC 1 cut(s) 392
MvaI CCWGG 2 cut(s) 291, 488
MwoI GCNNNNNNNGC 9 cut(s) 87, 668, 806, 809, 944, 1081, 1121, 1282, 1291
NciI CCSGG 1 cut(s) 84
NcoI CCATGG 1 cut(s) 1416
NlaIII CATG 9 cut(s) 788, 1013, 1022, 1067, 1088, 1210, 1232, 1420, 1493
NlaIV GGNNCC 1 cut(s) 954
NmeAIII GCCGAG 1 cut(s) 651
NmuCI GTSAC 1 cut(s) 295
NspV TTCGAA 2 cut(s) 602, 740
PcsI WCGNNNNNNNCGW 1 cut(s) 367
PctI GAATGC 1 cut(s) 392
PdmI GAANNNNTTC 2 cut(s) 285, 608
PfeI GAWTC 1 cut(s) 514
PfoI TCCNGGA 1 cut(s) 289
PinAI ACCGGT 1 cut(s) 445
Ple19I CGATCG 1 cut(s) 1241
PleI GAGTC 1 cut(s) 1414
PmaCI CACGTG 1 cut(s) 975
PmlI CACGTG 1 cut(s) 975
PpsI GAGTC 1 cut(s) 1414
Ppu21I YACGTR 1 cut(s) 975
Psp124BI GAGCTC 2 cut(s) 594, 1368
Psp6I CCWGG 2 cut(s) 289, 486
PspCI CACGTG 1 cut(s) 975
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 2 cut(s) 289, 486
PspN4I GGNNCC 1 cut(s) 954
PspPI GGNCC 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 1352
PsuI RGATCY 1 cut(s) 982
PvuI CGATCG 1 cut(s) 1241
RsaI GTAC 4 cut(s) 554, 733, 1059, 1451
RsaNI GTAC 4 cut(s) 553, 732, 1058, 1450
RseI CAYNNNNRTG 1 cut(s) 1083
SacI GAGCTC 2 cut(s) 594, 1368
SaqAI TTAA 3 cut(s) 677, 819, 839
Sau96I GGNCC 1 cut(s) 490
ScaI AGTACT 1 cut(s) 1451
SchI GAGTC 1 cut(s) 1415
ScrFI CCNGG 3 cut(s) 84, 291, 488
SduI GDGCHC 3 cut(s) 83, 594, 1368
SfaNI GCATC 6 cut(s) 28, 534, 974, 1210, 1330, 1358
SfuI TTCGAA 2 cut(s) 602, 740
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 1083
SmlI CTYRAG 2 cut(s) 813, 1428
SmoI CTYRAG 2 cut(s) 813, 1428
Sse9I AATT 1 cut(s) 143
SsiI CCGC 6 cut(s) 90, 408, 798, 1121, 1175, 1194
SspMI CTAG 2 cut(s) 300, 560
SstI GAGCTC 2 cut(s) 594, 1368
StyD4I CCNGG 3 cut(s) 82, 289, 486
StyI CCWWGG 2 cut(s) 339, 1416
TaaI ACNGT 1 cut(s) 713
TaiI ACGT 1 cut(s) 977
TaqI TCGA 9 cut(s) 71, 517, 602, 740, 775, 915, 1212, 1241, 1388
TasI AATT 1 cut(s) 143
TatI WGTACW 2 cut(s) 731, 1449
TauI GCSGC 1 cut(s) 1124
TfiI GAWTC 1 cut(s) 514
Tru1I TTAA 3 cut(s) 677, 819, 839
Tru9I TTAA 3 cut(s) 677, 819, 839
TscAI CASTG 2 cut(s) 83, 718
TseFI GTSAC 1 cut(s) 295
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 8 cut(s) 17, 223, 854, 995, 1022, 1175, 1374, 1460
TspRI CASTG 2 cut(s) 83, 718
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 1 cut(s) 143
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 127
XmnI GAANNNNTTC 2 cut(s) 285, 608
XspI CTAG 2 cut(s) 300, 560
ZrmI AGTACT 1 cut(s) 1451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.