Rw2G006200

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007454
Physical Location & Seq
Forward (+)
5977212 .. 5982513
5302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006200.1

Sequence Viewer

Length: 1479 bp
ATGAACAAGAGTAATATCAGAAGCTCTGATCTCATTGATGCCAAGCTTGAAGAACACCAGATGTGTGGATCGAAGCAGTGCCCCGGTTGTGGACACAAACTTGAAGGAAAGCCGGACTGGTTAGGTCTACCAGCAGGAGTGAAATTTGACCCAACAGACCAAGAACTGATTGAACACCTTGAAGCAAAGGTAGAAGCCAAAGACACAAAGTCTCACCCTTTGATTGATGAGTTCATTCCTACCATTGAAGGAGAAGATGGCATTTGTTACACCCATCCTGAAAAACTTCCTGGAGTGACTAGAGATGGGTTGAGCAGGCACTTCTTCCACAGACCCTCCAAGGCTTACACAACCGGGACAAGAAAGAGGCGAAAAATCCAAACAGAATGTGACCACTTGCAAGCCGGCGGGGAGACACGGTGGCACAAGACCGGCAAAACCAGGCCGGTGATGGTGAATGGCAAGCAAAAAGGGTGCAAGAAAATCCTGGTCCTCTACACAAACTTTGGGAAGAATCGAAAACCAGAGAAGACGAACTGGGTGATGCACCAGTACCATCTGGGCCAGCACGAGGAGGAGAAGGAAGGGGAGCTTGTAGTTTCGAAGATATTCTATCAGACGCAGCCGAGACAATGCAACTGGTCGTCCGAGAGAACAAGTGCTGCAAGTGCAAGTTTAACTGGTGAAGGAAGTAGTGATTTGAGAAGAGATAGTGGTGGTAGTGGGAGTTGTACTTCTTCTAAGGAAATAATAGTATCCCATAATACTCATCTTCGGGATCAGCATGATCAAATGTCCGCAGCAGCAGCAGCTCAAGTTAATGTCATTGCTCAGCCATTAACAAGTTACAATTCCATAGATATGCATCAGTTGAAGTCCGACCATTTCAGCTTTGTCCCATTCAGAAAAAGCTTCGATGAGGTGGGCATAGGAGAGGCTTCAACGGCAAGGGAACCCAGCATTGCATCTGGCACGTGCGAAGAGCTGCGTGAGAGACCACAGCTTCATCATGTGGCACATGATCAACTGCTACATCATCACCAACATCAACAACAAGTACACCATGAGCAACAGCAACATCAGCATGGCCAATCACATCATCAACAACAAATAGCAACGGCGGCCAGCTTTCACATCAGTCGGCCTTCACACCCCATTTCCACAATCATCTCTCCGCCTCCTCTTCATCACACCGGCACCTCCATCATTCTCGATCAGGATGCGTACCATGTCTCCCGATCAATAATGCTCCAAAACGAACATTTCCAGCAGCAGCAACAACAACAGCAGCAGCAACAACAACATCATAAACAGATGGGAGGGAGGTCAGCATCTGGCTTGGAGGAGCTCATAATGGCCTGCACTTCATCGAGTAATGTCAAAGAAGAGTCATCATCCATGGCAAACCCTCAAGAGGCAGAGTGGATGAAGTACTCTTCATTCTGGCCTGACCCTGACAACCCAGATCATCATGGATAG

Protein Analysis

492

Amino Acids

55.5

Weight (kDa)

6.65

Isoelectric Point (pI)

57.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 44 - 187 1.5e-25 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1196
AccI GTMKAC 1 cut(s) 127
AciI CCGC 4 cut(s) 408, 798, 1121, 1175
AclWI GGATC 2 cut(s) 76, 786
AcoI YGGCCR 2 cut(s) 1087, 1122
AcsI RAATTY 1 cut(s) 143
AcvI CACGTG 1 cut(s) 975
AfaI GTAC 5 cut(s) 554, 733, 1059, 1226, 1433
AfiI CCNNNNNNNGG 3 cut(s) 89, 571, 1414
AgsI TTSAA 7 cut(s) 50, 104, 173, 182, 248, 874, 942
AhdI GACNNNNNGTC 1 cut(s) 208
AjnI CCWGG 3 cut(s) 289, 440, 486
Alw21I GWGCWC 1 cut(s) 1350
Alw26I GTCTC 5 cut(s) 216, 407, 622, 988, 1237
AlwI GGATC 2 cut(s) 76, 786
AlwNI CAGNNNCTG 1 cut(s) 1334
AoxI GGCC 7 cut(s) 443, 562, 1087, 1122, 1142, 1356, 1445
ApoI RAATTY 1 cut(s) 143
Asp700I GAANNNNTTC 2 cut(s) 285, 608
AspS9I GGNCC 2 cut(s) 490, 562
AsuC2I CCSGG 2 cut(s) 84, 355
AsuHPI GGTGA 6 cut(s) 206, 460, 466, 553, 695, 1031
AsuII TTCGAA 1 cut(s) 602
AvaII GGWCC 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 83
BalI TGGCCA 1 cut(s) 1089
BanI GGYRCC 1 cut(s) 1196
BanII GRGCYC 1 cut(s) 1350
BauI CACGAG 1 cut(s) 569
BbrPI CACGTG 1 cut(s) 975
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 1350
BccI CCATC 7 cut(s) 251, 282, 299, 445, 564, 1211, 1309
BceAI ACGGC 2 cut(s) 960, 1134
BcgI CGANNNNNNTGC 2 cut(s) 1202, 1236
BciT130I CCWGG 3 cut(s) 291, 442, 488
BciVI GTATCC 1 cut(s) 766
BclI TGATCA 2 cut(s) 787, 1021
BcnI CCSGG 2 cut(s) 84, 355
BcoDI GTCTC 5 cut(s) 216, 407, 622, 988, 1237
BfaI CTAG 1 cut(s) 300
BfuI GTATCC 1 cut(s) 766
BlpI GCTNAGC 1 cut(s) 831
BmcAI AGTACT 1 cut(s) 1433
Bme1390I CCNGG 5 cut(s) 84, 291, 355, 442, 488
Bme18I GGWCC 1 cut(s) 490
BmeRI GACNNNNNGTC 1 cut(s) 208
BmgT120I GGNCC 2 cut(s) 490, 562
BmiI GGNNCC 2 cut(s) 954, 1198
BmrFI CCNGG 5 cut(s) 84, 291, 355, 442, 488
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 6 cut(s) 28, 534, 874, 974, 1210, 1340
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 2 cut(s) 798, 1395
BpuMI CCSGG 2 cut(s) 84, 355
BsaAI YACGTR 1 cut(s) 975
BsaBI GATNNNNATC 1 cut(s) 864
BsaI GGTCTC 1 cut(s) 988
BsaJI CCNNGG 3 cut(s) 82, 339, 1398
BsaXI ACNNNNNCTCC 6 cut(s) 320, 350, 404, 434, 1217, 1247
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 571, 1414
Bse118I RCCGGY 4 cut(s) 404, 431, 445, 1193
Bse1I ACTGG 5 cut(s) 122, 542, 550, 644, 685
Bse3DI GCAATG 2 cut(s) 825, 960
Bse8I GATNNNNATC 1 cut(s) 864
BseBI CCWGG 3 cut(s) 291, 442, 488
BseDI CCNNGG 3 cut(s) 82, 339, 1398
BseGI GGATG 4 cut(s) 274, 1225, 1394, 1431
BseJI GATNNNNATC 1 cut(s) 864
BseLI CCNNNNNNNGG 3 cut(s) 89, 571, 1414
BseMI GCAATG 2 cut(s) 825, 960
BseMII CTCAG 1 cut(s) 845
BseNI ACTGG 5 cut(s) 122, 542, 550, 644, 685
BseRI GAGGAG 4 cut(s) 587, 590, 1170, 1358
BseSI GKGCMC 1 cut(s) 83
BseYI CCCAGC 1 cut(s) 956
BsgI GTGCAG 1 cut(s) 1345
BshFI GGCC 7 cut(s) 445, 564, 1089, 1124, 1144, 1358, 1447
BshNI GGYRCC 1 cut(s) 1196
BsiHKAI GWGCWC 1 cut(s) 1350
BsiSI CCGG 7 cut(s) 84, 113, 354, 405, 432, 446, 1194
BslFI GGGAC 2 cut(s) 370, 881
BslI CCNNNNNNNGG 3 cut(s) 89, 571, 1414
BsmAI GTCTC 5 cut(s) 216, 407, 622, 988, 1237
BsmFI GGGAC 2 cut(s) 370, 881
BsnI GGCC 7 cut(s) 445, 564, 1089, 1124, 1144, 1358, 1447
Bso31I GGTCTC 1 cut(s) 988
Bsp119I TTCGAA 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 83, 1350
Bsp143I GATC 8 cut(s) 28, 68, 778, 787, 1021, 1213, 1238, 1465
Bsp1720I GCTNAGC 1 cut(s) 831
Bsp19I CCATGG 1 cut(s) 1398
BspACI CCGC 4 cut(s) 408, 798, 1121, 1175
BspANI GGCC 7 cut(s) 445, 564, 1089, 1124, 1144, 1358, 1447
BspCNI CTCAG 1 cut(s) 844
BspLI GGNNCC 2 cut(s) 954, 1198
BspPI GGATC 2 cut(s) 76, 786
BspQI GCTCTTC 1 cut(s) 975
BspT104I TTCGAA 1 cut(s) 602
BspT107I GGYRCC 1 cut(s) 1196
BspTNI GGTCTC 1 cut(s) 988
BsrDI GCAATG 2 cut(s) 825, 960
BsrFI RCCGGY 4 cut(s) 404, 431, 445, 1193
BsrI ACTGG 5 cut(s) 122, 542, 550, 644, 685
BssAI RCCGGY 4 cut(s) 404, 431, 445, 1193
BssECI CCNNGG 3 cut(s) 82, 339, 1398
BssMI GATC 8 cut(s) 28, 68, 778, 787, 1021, 1213, 1238, 1465
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 2 cut(s) 339, 1398
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 3 cut(s) 291, 442, 488
Bst4CI ACNGT 1 cut(s) 420
Bst6I CTCTTC 5 cut(s) 700, 975, 1188, 1380, 1441
BstBAI YACGTR 1 cut(s) 975
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 7 cut(s) 317, 402, 406, 464, 566, 1126, 1360
BstDEI CTNAG 2 cut(s) 741, 831
BstDSI CCRYGG 1 cut(s) 1398
BstF5I GGATG 4 cut(s) 274, 1225, 1394, 1431
BstKTI GATC 8 cut(s) 31, 71, 781, 790, 1024, 1216, 1241, 1468
BstMAI GTCTC 5 cut(s) 216, 407, 622, 988, 1237
BstMBI GATC 8 cut(s) 28, 68, 778, 787, 1021, 1213, 1238, 1465
BstMWI GCNNNNNNNGC 6 cut(s) 668, 806, 809, 944, 1081, 1121
BstNI CCWGG 3 cut(s) 291, 442, 488
BstSCI CCNGG 5 cut(s) 82, 289, 353, 440, 486
BstSLI GKGCMC 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 536
BstXI CCANNNNNNTGG 1 cut(s) 65
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 7 cut(s) 445, 564, 1089, 1124, 1144, 1358, 1447
BtgI CCRYGG 1 cut(s) 1398
BtsCI GGATG 4 cut(s) 274, 1225, 1394, 1431
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 1 cut(s) 83
Cac8I GCNNGC 7 cut(s) 317, 402, 406, 464, 566, 1126, 1360
CaiI CAGNNNCTG 1 cut(s) 1334
Cfr10I RCCGGY 4 cut(s) 404, 431, 445, 1193
Cfr13I GGNCC 2 cut(s) 490, 562
CseI GACGC 1 cut(s) 628
Csp6I GTAC 5 cut(s) 553, 732, 1058, 1225, 1432
CviAII CATG 8 cut(s) 785, 1010, 1019, 1064, 1085, 1229, 1399, 1472
CviQI GTAC 5 cut(s) 553, 732, 1058, 1225, 1432
DdeI CTNAG 2 cut(s) 741, 831
DpnI GATC 8 cut(s) 30, 70, 780, 789, 1023, 1215, 1240, 1467
DpnII GATC 8 cut(s) 28, 68, 778, 787, 1021, 1213, 1238, 1465
DriI GACNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 2 cut(s) 1087, 1122
Eam1104I CTCTTC 5 cut(s) 700, 975, 1188, 1380, 1441
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 5 cut(s) 700, 975, 1188, 1380, 1441
EciI GGCGGA 1 cut(s) 1164
Ecl136II GAGCTC 1 cut(s) 1348
Eco130I CCWWGG 2 cut(s) 339, 1398
Eco24I GRGCYC 1 cut(s) 1350
Eco31I GGTCTC 1 cut(s) 988
Eco47I GGWCC 1 cut(s) 490
Eco53kI GAGCTC 1 cut(s) 1348
Eco72I CACGTG 1 cut(s) 975
EcoICRI GAGCTC 1 cut(s) 1348
EcoRII CCWGG 3 cut(s) 289, 440, 486
EcoT14I CCWWGG 2 cut(s) 339, 1398
EcoT22I ATGCAT 1 cut(s) 867
EcoT38I GRGCYC 1 cut(s) 1350
ErhI CCWWGG 2 cut(s) 339, 1398
FaeI CATG 8 cut(s) 788, 1013, 1022, 1067, 1088, 1232, 1402, 1475
FalI AAGNNNNNCTT 2 cut(s) 576, 608
FaqI GGGAC 2 cut(s) 370, 881
FatI CATG 8 cut(s) 784, 1009, 1018, 1063, 1084, 1228, 1398, 1471
FauI CCCGC 1 cut(s) 401
FbaI TGATCA 2 cut(s) 787, 1021
FblI GTMKAC 1 cut(s) 127
FokI GGATG 4 cut(s) 261, 1232, 1381, 1438
FriOI GRGCYC 1 cut(s) 1350
FspBI CTAG 1 cut(s) 300
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 7 cut(s) 445, 564, 1089, 1124, 1144, 1358, 1447
HapII CCGG 7 cut(s) 84, 113, 354, 405, 432, 446, 1194
HgaI GACGC 1 cut(s) 628
Hin1II CATG 8 cut(s) 788, 1013, 1022, 1067, 1088, 1232, 1402, 1475
HindIII AAGCTT 2 cut(s) 44, 910
HinfI GANTC 2 cut(s) 514, 1388
HpaII CCGG 7 cut(s) 84, 113, 354, 405, 432, 446, 1194
HphI GGTGA 6 cut(s) 206, 460, 466, 553, 695, 1031
Hpy166II GTNNAC 3 cut(s) 92, 128, 1060
Hpy188I TCNGA 6 cut(s) 20, 28, 618, 649, 880, 905
Hpy188III TCNNGA 6 cut(s) 278, 776, 1211, 1217, 1236, 1412
Hpy8I GTNNAC 3 cut(s) 92, 128, 1060
HpyAV CCTTC 6 cut(s) 98, 242, 574, 578, 680, 1155
HpyCH4III ACNGT 1 cut(s) 420
HpyCH4IV ACGT 1 cut(s) 974
HpyCH4V TGCA 9 cut(s) 400, 477, 547, 636, 665, 671, 865, 965, 1362
HpyF10VI GCNNNNNNNGC 6 cut(s) 668, 806, 809, 944, 1081, 1121
HpyF3I CTNAG 2 cut(s) 741, 831
HpySE526I ACGT 1 cut(s) 974
Hsp92II CATG 8 cut(s) 788, 1013, 1022, 1067, 1088, 1232, 1402, 1475
KroI GCCGGC 1 cut(s) 404
KroNI GCCGGC 1 cut(s) 406
Ksp22I TGATCA 2 cut(s) 787, 1021
Kzo9I GATC 8 cut(s) 28, 68, 778, 787, 1021, 1213, 1238, 1465
LguI GCTCTTC 1 cut(s) 975
LmnI GCTCC 3 cut(s) 589, 1254, 1345
LweI GCATC 6 cut(s) 28, 534, 874, 974, 1210, 1340
MaeI CTAG 1 cut(s) 300
MaeII ACGT 1 cut(s) 974
MaeIII GTNAC 4 cut(s) 266, 295, 389, 845
MalI GATC 8 cut(s) 30, 70, 780, 789, 1023, 1215, 1240, 1467
MboI GATC 8 cut(s) 28, 68, 778, 787, 1021, 1213, 1238, 1465
MhlI GDGCHC 2 cut(s) 83, 1350
MlsI TGGCCA 1 cut(s) 1089
MluCI AATT 2 cut(s) 143, 850
MluNI TGGCCA 1 cut(s) 1089
MlyI GAGTC 1 cut(s) 1397
MmeI TCCRAC 1 cut(s) 903
Mox20I TGGCCA 1 cut(s) 1089
Mph1103I ATGCAT 1 cut(s) 867
MroNI GCCGGC 1 cut(s) 404
MroXI GAANNNNTTC 2 cut(s) 285, 608
MscI TGGCCA 1 cut(s) 1089
MseI TTAA 3 cut(s) 677, 819, 839
MslI CAYNNNNRTG 2 cut(s) 860, 1083
Msp20I TGGCCA 1 cut(s) 1089
MspI CCGG 7 cut(s) 84, 113, 354, 405, 432, 446, 1194
MspR9I CCNGG 5 cut(s) 84, 291, 355, 442, 488
MvaI CCWGG 3 cut(s) 291, 442, 488
MwoI GCNNNNNNNGC 6 cut(s) 668, 806, 809, 944, 1081, 1121
NaeI GCCGGC 1 cut(s) 406
NciI CCSGG 2 cut(s) 84, 355
NcoI CCATGG 1 cut(s) 1398
NdeII GATC 8 cut(s) 28, 68, 778, 787, 1021, 1213, 1238, 1465
NgoMIV GCCGGC 1 cut(s) 404
NlaIII CATG 8 cut(s) 788, 1013, 1022, 1067, 1088, 1232, 1402, 1475
NlaIV GGNNCC 2 cut(s) 954, 1198
NmeAIII GCCGAG 1 cut(s) 651
NmuCI GTSAC 2 cut(s) 295, 389
NsiI ATGCAT 1 cut(s) 867
NspV TTCGAA 1 cut(s) 602
PciSI GCTCTTC 1 cut(s) 975
PdiI GCCGGC 1 cut(s) 406
PdmI GAANNNNTTC 2 cut(s) 285, 608
PfeI GAWTC 1 cut(s) 514
PfoI TCCNGGA 1 cut(s) 289
PleI GAGTC 1 cut(s) 1396
PmaCI CACGTG 1 cut(s) 975
PmlI CACGTG 1 cut(s) 975
PpsI GAGTC 1 cut(s) 1396
Ppu21I YACGTR 1 cut(s) 975
Psp124BI GAGCTC 1 cut(s) 1350
Psp6I CCWGG 3 cut(s) 289, 440, 486
PspCI CACGTG 1 cut(s) 975
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 3 cut(s) 289, 440, 486
PspN4I GGNNCC 2 cut(s) 954, 1198
PspPI GGNCC 2 cut(s) 490, 562
PstNI CAGNNNCTG 1 cut(s) 1334
RsaI GTAC 5 cut(s) 554, 733, 1059, 1226, 1433
RsaNI GTAC 5 cut(s) 553, 732, 1058, 1225, 1432
RseI CAYNNNNRTG 2 cut(s) 860, 1083
SacI GAGCTC 1 cut(s) 1350
SapI GCTCTTC 1 cut(s) 975
SaqAI TTAA 3 cut(s) 677, 819, 839
Sau3AI GATC 8 cut(s) 28, 68, 778, 787, 1021, 1213, 1238, 1465
Sau96I GGNCC 2 cut(s) 490, 562
ScaI AGTACT 1 cut(s) 1433
SchI GAGTC 1 cut(s) 1397
ScrFI CCNGG 5 cut(s) 84, 291, 355, 442, 488
SduI GDGCHC 2 cut(s) 83, 1350
SfaNI GCATC 6 cut(s) 28, 534, 874, 974, 1210, 1340
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 2 cut(s) 860, 1083
SmlI CTYRAG 2 cut(s) 813, 1410
SmoI CTYRAG 2 cut(s) 813, 1410
Sse9I AATT 2 cut(s) 143, 850
SsiI CCGC 4 cut(s) 408, 798, 1121, 1175
SspMI CTAG 1 cut(s) 300
SstI GAGCTC 1 cut(s) 1350
StyD4I CCNGG 5 cut(s) 82, 289, 353, 440, 486
StyI CCWWGG 2 cut(s) 339, 1398
TaaI ACNGT 1 cut(s) 420
TaiI ACGT 1 cut(s) 977
TaqI TCGA 6 cut(s) 71, 517, 602, 915, 1212, 1370
TasI AATT 2 cut(s) 143, 850
TatI WGTACW 3 cut(s) 731, 1057, 1431
TauI GCSGC 1 cut(s) 1124
TfiI GAWTC 1 cut(s) 514
Tru1I TTAA 3 cut(s) 677, 819, 839
Tru9I TTAA 3 cut(s) 677, 819, 839
TscAI CASTG 1 cut(s) 83
TseFI GTSAC 2 cut(s) 295, 389
Tsp45I GTSAC 2 cut(s) 295, 389
TspDTI ATGAA 7 cut(s) 17, 223, 995, 1175, 1356, 1428, 1442
TspRI CASTG 1 cut(s) 83
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 1 cut(s) 143
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 127
XmnI GAANNNNTTC 2 cut(s) 285, 608
XspI CTAG 1 cut(s) 300
ZrmI AGTACT 1 cut(s) 1433
Zsp2I ATGCAT 1 cut(s) 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.