Rroxscaffold_2G00149300

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007454
Physical Location & Seq
Forward (+)
86905804 .. 86911198
5395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149300.1

Sequence Viewer

Length: 1482 bp
ATGAATAAGAGTAATATCAGAAGCTCTGATCTCATTGATGCCAAGCTTGAAGAACACCAGATGTGTGGATCGAAGCAGTGCCCTGGTTGTGGACACAAACTTGAAGGAAAGCCGGACTGGTTAGGTCTACCAGCAGGAGTGAAATTTGACCCAACAGACCAAGAACTGATTGAACACCTTGAAGCAAAGGTAGAAGCCAAAGACACAAAGTCTCACCCTTTGATTGATGAGTTCATTCCTACCATTGAAGGAGAAGATGGCATTTGTTACACCCATCCTGAAAAACTTCCTGGAGTGACTAGAGATGGGTTGAGCAGGCACTTCTTCCACAGACCGTCCAAGGCTTACACAACCGGGACAAGAAAGAGGCGAAAAATCCAAACAGAATGTGACCACTTGCAAGCCGGCGGGGAGACACGGTGGCACAAGACCGGCAAAACCAGGCCGGTGATGGTGAATGGCAAGCAAAAAGGGTGCAAGAAAATCCTGGTCCTCTACACAAACTTTGGGAAGAATCGAAAACCAGAGAAGACGAACTGGGTGATGCACCAGTACCATCTGGGCCAGCACGAGGAGGAGAAGGAAGGGGAGCTCGTAGTTTCGAAGATATTCTATCAGACGCAGCCGAGACAATGCAACTGGTCGTCCGAGAGAACAAGTGCTGCAAGTGCAAGTTTAACCGGTGAAGGAAGTAGTGATTTGAGAAGAGATAGTGGTGGTAGTGGGAGTTGTACTTCTTCTAAGGAAATAATAGTATCCCATAATACTCATCTTCGGGATCAGCATGATCAAATGTCCGCAGCAGCAGCAGCTCAAGTTAATGTCATTGCTCAGCCATTAACAAGTTACAATTCCATAGATATGCATCAGTTGAAGTCCGACCATTTCAGCTTGCTCACATTCAGAAAAAGCTTCGATGAGGTGGGGATAGGAGAGGCTTCAACGGCAAGGGAACCCAGCATTGCATCTGGCACATGCGAAGAGCTGCGTGAGAGACCACAGCTTCATCATGTGGCACATGATCAACTGCTACATCATCACCAACATCAACAAGTACACCATGAGCAACAGCAACATCAGCATGGCCAATCACATCATCAACAACAAATAGCAACGGCGGCCAGCTTTCACATCAGTCGGCCTTCACACCCCATTTCCACAATCATCTCTCCGCCTCCTCTTCATCACACTGGCACCTCCATCATTCTCGATCAGGATGCGTACCATGTCTCCCGATCAATAATGCTCCAAAACGAACATTTCCAGCAGCAGCAACAACAACAACAGCAACAGCAGCAACAACAACATCATAAACAGATGGGAGGGAGGTCAGCATCTGGCTTGGAGGAGCTCATAATGGCCTGCACTTCATCGAGTAATGTCAAAGAAGAGTCATCATCCATGGCAAACCCTCAAGAGGCAGAGTGGATGAAGTACTCTTCATTCTGGCCTGACCCTGACAACCCAGATCATCATGGATAG

Protein Analysis

493

Amino Acids

55.62

Weight (kDa)

6.65

Isoelectric Point (pI)

57.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 44 - 187 1.5e-25 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1193
AccI GTMKAC 1 cut(s) 127
AciI CCGC 4 cut(s) 408, 798, 1118, 1172
AclWI GGATC 2 cut(s) 76, 786
AcoI YGGCCR 2 cut(s) 1084, 1119
AcsI RAATTY 1 cut(s) 143
AfaI GTAC 5 cut(s) 554, 733, 1056, 1223, 1436
AfiI CCNNNNNNNGG 3 cut(s) 89, 571, 1417
AgeI ACCGGT 1 cut(s) 680
AgsI TTSAA 7 cut(s) 50, 104, 173, 182, 248, 874, 942
AhdI GACNNNNNGTC 1 cut(s) 208
AjnI CCWGG 4 cut(s) 82, 289, 440, 486
Alw21I GWGCWC 2 cut(s) 594, 1353
Alw26I GTCTC 5 cut(s) 216, 407, 622, 988, 1234
AlwI GGATC 2 cut(s) 76, 786
AlwNI CAGNNNCTG 1 cut(s) 1337
AoxI GGCC 7 cut(s) 443, 562, 1084, 1119, 1139, 1359, 1448
ApoI RAATTY 1 cut(s) 143
AsiGI ACCGGT 1 cut(s) 680
Asp700I GAANNNNTTC 2 cut(s) 285, 608
AspS9I GGNCC 2 cut(s) 490, 562
AsuC2I CCSGG 1 cut(s) 355
AsuHPI GGTGA 6 cut(s) 206, 460, 466, 553, 695, 1031
AsuII TTCGAA 1 cut(s) 602
AvaII GGWCC 1 cut(s) 490
BaeGI GKGCMC 1 cut(s) 83
BalI TGGCCA 1 cut(s) 1086
BanI GGYRCC 1 cut(s) 1193
BanII GRGCYC 2 cut(s) 594, 1353
BauI CACGAG 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 594, 1353
BccI CCATC 7 cut(s) 251, 282, 299, 445, 564, 1208, 1312
BceAI ACGGC 2 cut(s) 960, 1131
BcgI CGANNNNNNTGC 2 cut(s) 1199, 1233
BciT130I CCWGG 4 cut(s) 84, 291, 442, 488
BciVI GTATCC 1 cut(s) 766
BclI TGATCA 2 cut(s) 787, 1021
BcnI CCSGG 1 cut(s) 355
BcoDI GTCTC 5 cut(s) 216, 407, 622, 988, 1234
BfaI CTAG 1 cut(s) 300
BfuI GTATCC 1 cut(s) 766
BlpI GCTNAGC 1 cut(s) 831
BmcAI AGTACT 1 cut(s) 1436
Bme1390I CCNGG 5 cut(s) 84, 291, 355, 442, 488
Bme18I GGWCC 1 cut(s) 490
BmeRI GACNNNNNGTC 1 cut(s) 208
BmgT120I GGNCC 2 cut(s) 490, 562
BmiI GGNNCC 2 cut(s) 954, 1195
BmrFI CCNGG 5 cut(s) 84, 291, 355, 442, 488
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 6 cut(s) 28, 534, 874, 974, 1207, 1343
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 1 cut(s) 602
BpuEI CTTGAG 2 cut(s) 798, 1398
BpuMI CCSGG 1 cut(s) 355
BsaBI GATNNNNATC 1 cut(s) 864
BsaI GGTCTC 1 cut(s) 988
BsaJI CCNNGG 3 cut(s) 82, 339, 1401
BsaWI WCCGGW 1 cut(s) 680
BsaXI ACNNNNNCTCC 4 cut(s) 404, 434, 1214, 1244
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 571, 1417
Bse118I RCCGGY 4 cut(s) 404, 431, 445, 680
Bse1I ACTGG 5 cut(s) 122, 542, 550, 644, 1195
Bse3DI GCAATG 2 cut(s) 825, 960
Bse8I GATNNNNATC 1 cut(s) 864
BseBI CCWGG 4 cut(s) 84, 291, 442, 488
BseDI CCNNGG 3 cut(s) 82, 339, 1401
BseGI GGATG 4 cut(s) 274, 1222, 1397, 1434
BseJI GATNNNNATC 1 cut(s) 864
BseLI CCNNNNNNNGG 3 cut(s) 89, 571, 1417
BseMI GCAATG 2 cut(s) 825, 960
BseMII CTCAG 1 cut(s) 845
BseNI ACTGG 5 cut(s) 122, 542, 550, 644, 1195
BseRI GAGGAG 4 cut(s) 587, 590, 1167, 1361
BseSI GKGCMC 1 cut(s) 83
BseYI CCCAGC 1 cut(s) 956
BsgI GTGCAG 1 cut(s) 1348
BshFI GGCC 7 cut(s) 445, 564, 1086, 1121, 1141, 1361, 1450
BshNI GGYRCC 1 cut(s) 1193
BshTI ACCGGT 1 cut(s) 680
BsiHKAI GWGCWC 2 cut(s) 594, 1353
BsiSI CCGG 6 cut(s) 113, 354, 405, 432, 446, 681
BslFI GGGAC 1 cut(s) 370
BslI CCNNNNNNNGG 3 cut(s) 89, 571, 1417
BsmAI GTCTC 5 cut(s) 216, 407, 622, 988, 1234
BsmFI GGGAC 1 cut(s) 370
BsnI GGCC 7 cut(s) 445, 564, 1086, 1121, 1141, 1361, 1450
Bso31I GGTCTC 1 cut(s) 988
Bsp119I TTCGAA 1 cut(s) 602
Bsp1286I GDGCHC 3 cut(s) 83, 594, 1353
Bsp143I GATC 8 cut(s) 28, 68, 778, 787, 1021, 1210, 1235, 1468
Bsp1720I GCTNAGC 1 cut(s) 831
Bsp19I CCATGG 1 cut(s) 1401
BspACI CCGC 4 cut(s) 408, 798, 1118, 1172
BspANI GGCC 7 cut(s) 445, 564, 1086, 1121, 1141, 1361, 1450
BspCNI CTCAG 1 cut(s) 844
BspLI GGNNCC 2 cut(s) 954, 1195
BspPI GGATC 2 cut(s) 76, 786
BspQI GCTCTTC 1 cut(s) 975
BspT104I TTCGAA 1 cut(s) 602
BspT107I GGYRCC 1 cut(s) 1193
BspTNI GGTCTC 1 cut(s) 988
BsrDI GCAATG 2 cut(s) 825, 960
BsrFI RCCGGY 4 cut(s) 404, 431, 445, 680
BsrI ACTGG 5 cut(s) 122, 542, 550, 644, 1195
BssAI RCCGGY 4 cut(s) 404, 431, 445, 680
BssECI CCNNGG 3 cut(s) 82, 339, 1401
BssMI GATC 8 cut(s) 28, 68, 778, 787, 1021, 1210, 1235, 1468
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 2 cut(s) 339, 1401
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 4 cut(s) 84, 291, 442, 488
Bst4CI ACNGT 2 cut(s) 336, 420
Bst6I CTCTTC 5 cut(s) 700, 975, 1185, 1383, 1444
BstBI TTCGAA 1 cut(s) 602
BstC8I GCNNGC 8 cut(s) 317, 402, 406, 464, 566, 893, 1123, 1363
BstDEI CTNAG 2 cut(s) 741, 831
BstDSI CCRYGG 1 cut(s) 1401
BstF5I GGATG 4 cut(s) 274, 1222, 1397, 1434
BstKTI GATC 8 cut(s) 31, 71, 781, 790, 1024, 1213, 1238, 1471
BstMAI GTCTC 5 cut(s) 216, 407, 622, 988, 1234
BstMBI GATC 8 cut(s) 28, 68, 778, 787, 1021, 1210, 1235, 1468
BstMWI GCNNNNNNNGC 7 cut(s) 668, 806, 809, 944, 1078, 1118, 1294
BstNI CCWGG 4 cut(s) 84, 291, 442, 488
BstNSI RCATGY 1 cut(s) 978
BstSCI CCNGG 5 cut(s) 82, 289, 353, 440, 486
BstSLI GKGCMC 1 cut(s) 83
BstV2I GAAGAC 1 cut(s) 536
BstXI CCANNNNNNTGG 1 cut(s) 65
BsuI GTATCC 1 cut(s) 766
BsuRI GGCC 7 cut(s) 445, 564, 1086, 1121, 1141, 1361, 1450
BtgI CCRYGG 1 cut(s) 1401
BtsCI GGATG 4 cut(s) 274, 1222, 1397, 1434
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 2 cut(s) 83, 1188
Cac8I GCNNGC 8 cut(s) 317, 402, 406, 464, 566, 893, 1123, 1363
CaiI CAGNNNCTG 1 cut(s) 1337
Cfr10I RCCGGY 4 cut(s) 404, 431, 445, 680
Cfr13I GGNCC 2 cut(s) 490, 562
CseI GACGC 1 cut(s) 628
Csp6I GTAC 5 cut(s) 553, 732, 1055, 1222, 1435
CspAI ACCGGT 1 cut(s) 680
CviAII CATG 9 cut(s) 785, 975, 1010, 1019, 1061, 1082, 1226, 1402, 1475
CviQI GTAC 5 cut(s) 553, 732, 1055, 1222, 1435
DdeI CTNAG 2 cut(s) 741, 831
DpnI GATC 8 cut(s) 30, 70, 780, 789, 1023, 1212, 1237, 1470
DpnII GATC 8 cut(s) 28, 68, 778, 787, 1021, 1210, 1235, 1468
DriI GACNNNNNGTC 1 cut(s) 208
EaeI YGGCCR 2 cut(s) 1084, 1119
Eam1104I CTCTTC 5 cut(s) 700, 975, 1185, 1383, 1444
Eam1105I GACNNNNNGTC 1 cut(s) 208
EarI CTCTTC 5 cut(s) 700, 975, 1185, 1383, 1444
EciI GGCGGA 1 cut(s) 1161
Ecl136II GAGCTC 2 cut(s) 592, 1351
Eco130I CCWWGG 2 cut(s) 339, 1401
Eco24I GRGCYC 2 cut(s) 594, 1353
Eco31I GGTCTC 1 cut(s) 988
Eco47I GGWCC 1 cut(s) 490
Eco53kI GAGCTC 2 cut(s) 592, 1351
EcoICRI GAGCTC 2 cut(s) 592, 1351
EcoRII CCWGG 4 cut(s) 82, 289, 440, 486
EcoT14I CCWWGG 2 cut(s) 339, 1401
EcoT22I ATGCAT 1 cut(s) 867
EcoT38I GRGCYC 2 cut(s) 594, 1353
ErhI CCWWGG 2 cut(s) 339, 1401
FaeI CATG 9 cut(s) 788, 978, 1013, 1022, 1064, 1085, 1229, 1405, 1478
FaqI GGGAC 1 cut(s) 370
FatI CATG 9 cut(s) 784, 974, 1009, 1018, 1060, 1081, 1225, 1401, 1474
FauI CCCGC 1 cut(s) 401
FbaI TGATCA 2 cut(s) 787, 1021
FblI GTMKAC 1 cut(s) 127
FokI GGATG 4 cut(s) 261, 1229, 1384, 1441
FriOI GRGCYC 2 cut(s) 594, 1353
FspBI CTAG 1 cut(s) 300
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 1 cut(s) 312
HaeIII GGCC 7 cut(s) 445, 564, 1086, 1121, 1141, 1361, 1450
HapII CCGG 6 cut(s) 113, 354, 405, 432, 446, 681
HgaI GACGC 1 cut(s) 628
Hin1II CATG 9 cut(s) 788, 978, 1013, 1022, 1064, 1085, 1229, 1405, 1478
HindIII AAGCTT 2 cut(s) 44, 910
HinfI GANTC 2 cut(s) 514, 1391
HpaII CCGG 6 cut(s) 113, 354, 405, 432, 446, 681
HphI GGTGA 6 cut(s) 206, 460, 466, 553, 695, 1031
Hpy166II GTNNAC 3 cut(s) 92, 128, 1057
Hpy188I TCNGA 6 cut(s) 20, 28, 618, 649, 880, 905
Hpy188III TCNNGA 6 cut(s) 278, 776, 1208, 1214, 1233, 1415
Hpy8I GTNNAC 3 cut(s) 92, 128, 1057
HpyAV CCTTC 6 cut(s) 98, 242, 574, 578, 680, 1152
HpyCH4III ACNGT 2 cut(s) 336, 420
HpyCH4V TGCA 9 cut(s) 400, 477, 547, 636, 665, 671, 865, 965, 1365
HpyF10VI GCNNNNNNNGC 7 cut(s) 668, 806, 809, 944, 1078, 1118, 1294
HpyF3I CTNAG 2 cut(s) 741, 831
Hsp92II CATG 9 cut(s) 788, 978, 1013, 1022, 1064, 1085, 1229, 1405, 1478
KroI GCCGGC 1 cut(s) 404
KroNI GCCGGC 1 cut(s) 406
Ksp22I TGATCA 2 cut(s) 787, 1021
Kzo9I GATC 8 cut(s) 28, 68, 778, 787, 1021, 1210, 1235, 1468
LguI GCTCTTC 1 cut(s) 975
LmnI GCTCC 3 cut(s) 589, 1251, 1348
LweI GCATC 6 cut(s) 28, 534, 874, 974, 1207, 1343
MaeI CTAG 1 cut(s) 300
MaeIII GTNAC 4 cut(s) 266, 295, 389, 845
MalI GATC 8 cut(s) 30, 70, 780, 789, 1023, 1212, 1237, 1470
MboI GATC 8 cut(s) 28, 68, 778, 787, 1021, 1210, 1235, 1468
MhlI GDGCHC 3 cut(s) 83, 594, 1353
MlsI TGGCCA 1 cut(s) 1086
MluCI AATT 2 cut(s) 143, 850
MluNI TGGCCA 1 cut(s) 1086
MlyI GAGTC 1 cut(s) 1400
MmeI TCCRAC 1 cut(s) 903
Mox20I TGGCCA 1 cut(s) 1086
Mph1103I ATGCAT 1 cut(s) 867
MroNI GCCGGC 1 cut(s) 404
MroXI GAANNNNTTC 2 cut(s) 285, 608
MscI TGGCCA 1 cut(s) 1086
MseI TTAA 3 cut(s) 677, 819, 839
MslI CAYNNNNRTG 2 cut(s) 860, 1080
Msp20I TGGCCA 1 cut(s) 1086
MspI CCGG 6 cut(s) 113, 354, 405, 432, 446, 681
MspR9I CCNGG 5 cut(s) 84, 291, 355, 442, 488
MvaI CCWGG 4 cut(s) 84, 291, 442, 488
MwoI GCNNNNNNNGC 7 cut(s) 668, 806, 809, 944, 1078, 1118, 1294
NaeI GCCGGC 1 cut(s) 406
NciI CCSGG 1 cut(s) 355
NcoI CCATGG 1 cut(s) 1401
NdeII GATC 8 cut(s) 28, 68, 778, 787, 1021, 1210, 1235, 1468
NgoMIV GCCGGC 1 cut(s) 404
NlaIII CATG 9 cut(s) 788, 978, 1013, 1022, 1064, 1085, 1229, 1405, 1478
NlaIV GGNNCC 2 cut(s) 954, 1195
NmeAIII GCCGAG 1 cut(s) 651
NmuCI GTSAC 2 cut(s) 295, 389
NsiI ATGCAT 1 cut(s) 867
NspI RCATGY 1 cut(s) 978
NspV TTCGAA 1 cut(s) 602
PciSI GCTCTTC 1 cut(s) 975
PdiI GCCGGC 1 cut(s) 406
PdmI GAANNNNTTC 2 cut(s) 285, 608
PfeI GAWTC 1 cut(s) 514
PfoI TCCNGGA 1 cut(s) 289
PinAI ACCGGT 1 cut(s) 680
PleI GAGTC 1 cut(s) 1399
PpsI GAGTC 1 cut(s) 1399
Psp124BI GAGCTC 2 cut(s) 594, 1353
Psp6I CCWGG 4 cut(s) 82, 289, 440, 486
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 4 cut(s) 82, 289, 440, 486
PspN4I GGNNCC 2 cut(s) 954, 1195
PspPI GGNCC 2 cut(s) 490, 562
PstNI CAGNNNCTG 1 cut(s) 1337
RsaI GTAC 5 cut(s) 554, 733, 1056, 1223, 1436
RsaNI GTAC 5 cut(s) 553, 732, 1055, 1222, 1435
RseI CAYNNNNRTG 2 cut(s) 860, 1080
SacI GAGCTC 2 cut(s) 594, 1353
SapI GCTCTTC 1 cut(s) 975
SaqAI TTAA 3 cut(s) 677, 819, 839
Sau3AI GATC 8 cut(s) 28, 68, 778, 787, 1021, 1210, 1235, 1468
Sau96I GGNCC 2 cut(s) 490, 562
ScaI AGTACT 1 cut(s) 1436
SchI GAGTC 1 cut(s) 1400
ScrFI CCNGG 5 cut(s) 84, 291, 355, 442, 488
SduI GDGCHC 3 cut(s) 83, 594, 1353
SfaNI GCATC 6 cut(s) 28, 534, 874, 974, 1207, 1343
SfuI TTCGAA 1 cut(s) 602
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 2 cut(s) 860, 1080
SmlI CTYRAG 2 cut(s) 813, 1413
SmoI CTYRAG 2 cut(s) 813, 1413
Sse9I AATT 2 cut(s) 143, 850
SsiI CCGC 4 cut(s) 408, 798, 1118, 1172
SspMI CTAG 1 cut(s) 300
SstI GAGCTC 2 cut(s) 594, 1353
StyD4I CCNGG 5 cut(s) 82, 289, 353, 440, 486
StyI CCWWGG 2 cut(s) 339, 1401
TaaI ACNGT 2 cut(s) 336, 420
TaqI TCGA 6 cut(s) 71, 517, 602, 915, 1209, 1373
TasI AATT 2 cut(s) 143, 850
TatI WGTACW 3 cut(s) 731, 1054, 1434
TauI GCSGC 1 cut(s) 1121
TfiI GAWTC 1 cut(s) 514
Tru1I TTAA 3 cut(s) 677, 819, 839
Tru9I TTAA 3 cut(s) 677, 819, 839
TscAI CASTG 2 cut(s) 83, 1195
TseFI GTSAC 2 cut(s) 295, 389
Tsp45I GTSAC 2 cut(s) 295, 389
TspDTI ATGAA 7 cut(s) 17, 223, 995, 1172, 1359, 1431, 1445
TspRI CASTG 2 cut(s) 83, 1195
VpaK11BI GGWCC 1 cut(s) 490
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 978
XcmI CCANNNNNNNNNTGG 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 127
XmnI GAANNNNTTC 2 cut(s) 285, 608
XspI CTAG 1 cut(s) 300
ZrmI AGTACT 1 cut(s) 1436
Zsp2I ATGCAT 1 cut(s) 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.