Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0007454

Physical Location & Seq

Genomic Coordinates Reverse (-)

5573852 .. 5579173

Gene Structure 5322 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1068700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1467 bp

ATGAATAAGAGTACTTTGGGGTCCATCAGAAGTTCTGATCTCATTGATGCAAAGCTTGAAGAGCATCAGATGTGCGGATCCAAGCAGTGTCCCGGTTGTGGACACAAACTCGAAGGAAAGCCGGACTGGTTAGGTCTACCAGCAGGAGTGAAATTTGATCCAACAGACCAAGAACTAATTGAACACCTTGAAGCAAAGGTAGAAGCCAAAAACTCCAAGTCTCACCCTTTGATTGATGAATTCATCCCTACAATTGAAGGAGAAGATGGAATTTGTTACACCCATCCTGAAAAACTTCCAGGGGTCACAAGAGATGGCTTGAGCAGGCACTTCTTCCATAGACCTTCAAAAGCCTACACAACTGGGACAAGAAAGAGAAGAAAAATCCAAACAGAATGTGACTTGCAAGGTGGGGAAACAAGATGGCACAAGACAGGCAAGACCAGGCCAGTCATGGTGAATGGAAAACAAAAAGGGTGCAAGAAAATTCTAGTCCTCTACACCAACTTTGGCAAAAACCGAAAACCCGAAAAGACGAATTGGGTGATGCACCAATACCATCTTGGGCAGCATGAGGAGGAGAAAGAAGGAGAGCTTGTGGTGTCAAAGATATTCTATCAGACTCAGCCAAGGCAATGCAACTGGTCTGATAGAGCGAGTGCAACTGGTGAAGGAAGCAGTGATATTGTGGCTACTAATAGCAGAAGAGATAGTGGGAGTGGGAGTTGTTCTTCTAAGGAAATATTACCTTCTCATCCTCATAGAGATCATGACCAAATAGCTGCAACAGCAGCAGCTGCGGTTGCAGCTGCTGCTCCAATCTCAAGCTACAGTGGCATTGACATTCATCAGTTGAAGTCAGATCACTTCAGCTTTGCCCCCTTCAGAAAAAGCTTTGATGAGGTGGGGATAGGAGGAGAGGCTTCAACTGTAAGGGAAGGGCTGCCATCTGGCACGTGCGAAGAGATACGAGAGCACCACCAGAGATCAGTACCAGTACAGCCTCATCATCACCATCATCATCATATAAGCCATGAACTAGTATCTGATCATCACGAGCAGCAGCAGCAACATGAACATCAACAGCAGCAGCAACTGCATCATCATCAACAAATTGCAACAGCTGCAGCTTTCCACATCAGCCGCCCTTCACATCCCATTTCCACCATCATCTCTCCACCTCCTCTCCACCACACTTCCATCATTCTTGATCAGGATTCCTACTCCCGGTTGATGCTCCAAAACGAAAATTTCCAGGCGCAGCAACAGCAGCAACACCCCCACCACCAACAGCATCATAAAATGGGAGCTAGGTCTGCATCTGGTTTGGAGGAACTAATAATGGGCTGCACTTCCAGCAGTAGTGATATCAAAGAAGAGTCATCGATGCCAAACCCTCAAGAGGCAGAGTGGATGAAGTACTCCTCCTTCTGGCCTGACCCTGACAACCCAGATCATCATGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000075 GO:0000077 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0006974 GO:0008150 GO:0009314 GO:0009628 GO:0009889 GO:0009987 GO:0010212 GO:0010332 GO:0010468 GO:0010556 GO:0010564 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031570 GO:0033043 GO:0033554 GO:0040020 GO:0043565 GO:0045786 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051445 GO:0051716 GO:0051726 GO:0051783 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2000241 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NAM

Protein Analysis

489

Amino Acids

54.88

Weight (kDa)

6.57

Isoelectric Point (pI)

52.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAM	PF02365	47 - 188	5e-26	No apical meristem (NAM) protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007454)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G29230 AT4G29230 AT4G29230
fragaria_vesca	FvH4_1g06530 FvH4_1g06530
malus_domestica	MD02G1068700.v1.1 MD15G1200200.v1.1
prunus_persica	Prupe.7G217000_v2.0.a1 Prupe.7G217000_v2.0.a1 Prupe.7G217000_v2.0.a1
pyrus_communis	pycom02g05440 pycom15g17790
rosa_chinensis	RchiOBHm_Chr2g0092281
rosa_laevigata	RLG00000016293
rosa_multiflora	Rmu_sc0003289.1_g000022
rosa_roxburghii	Rroxscaffold_2G00149300
rosa_rugosa	Rorug02G0024400 Rorug02G0024500 Rorug02G0024600 Rorug02G0024600
rosa_samantha	Rh2AG070300 Rh2BG069800 Rh2CG071000 Rh2DG068800
rosa_wichuraiana	Rw2G006200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	136
AciI	CCGC	3 cut(s)	75, 800, 1144
AclWI	GGATC	3 cut(s)	72, 85, 152
AcsI	RAATTY	5 cut(s)	152, 239, 270, 486, 1249
AcuI	CTGAAG	2 cut(s)	853, 868
AcvI	CACGTG	1 cut(s)	957
AfaI	GTAC	4 cut(s)	13, 993, 999, 1421
AfiI	CCNNNNNNNGG	4 cut(s)	98, 1227, 1402, 1431
AgsI	TTSAA	7 cut(s)	59, 182, 191, 257, 348, 856, 927
AhlI	ACTAGT	1 cut(s)	1039
AjnI	CCWGG	3 cut(s)	298, 443, 1254
Alw21I	GWGCWC	1 cut(s)	978
Alw26I	GTCTC	1 cut(s)	225
AlwI	GGATC	3 cut(s)	72, 85, 152
AlwNI	CAGNNNCTG	3 cut(s)	797, 812, 1096
AoxI	GGCC	2 cut(s)	446, 1433
ApoI	RAATTY	5 cut(s)	152, 239, 270, 486, 1249
Asp700I	GAANNNNTTC	1 cut(s)	294
AspLEI	GCGC	1 cut(s)	1261
AspS9I	GGNCC	1 cut(s)	21
AsuC2I	CCSGG	2 cut(s)	93, 1228
AsuHPI	GGTGA	5 cut(s)	215, 469, 556, 680, 1004
AvaII	GGWCC	1 cut(s)	21
BamHI	GGATCC	1 cut(s)	77
BarI	GAAGNNNNNNTAC	2 cut(s)	1412, 1444
BauI	CACGAG	1 cut(s)	1055
BbrPI	CACGTG	1 cut(s)	957
Bbv12I	GWGCWC	1 cut(s)	978
BciT130I	CCWGG	3 cut(s)	300, 445, 1256
BclI	TGATCA	2 cut(s)	1048, 1210
BcnI	CCSGG	2 cut(s)	93, 1228
BcoDI	GTCTC	1 cut(s)	225
BcuI	ACTAGT	1 cut(s)	1039
BfaI	CTAG	3 cut(s)	491, 1040, 1311
BfmI	CTRYAG	2 cut(s)	829, 1125
BmcAI	AGTACT	2 cut(s)	13, 1421
Bme1390I	CCNGG	5 cut(s)	93, 300, 445, 1228, 1256
Bme18I	GGWCC	1 cut(s)	21
BmgT120I	GGNCC	1 cut(s)	21
BmiI	GGNNCC	2 cut(s)	22, 79
BmrFI	CCNGG	5 cut(s)	93, 300, 445, 1228, 1256
BmrI	ACTGGG	1 cut(s)	372
BmsI	GCATC	8 cut(s)	37, 73, 537, 1108, 1224, 1303, 1328, 1377
BmuI	ACTGGG	1 cut(s)	372
BpuEI	CTTGAG	3 cut(s)	340, 808, 1383
BpuMI	CCSGG	2 cut(s)	93, 1228
Bsa29I	ATCGAT	1 cut(s)	1385
BsaAI	YACGTR	1 cut(s)	957
BsaJI	CCNNGG	2 cut(s)	299, 629
BsaXI	ACNNNNNCTCC	6 cut(s)	582, 612, 709, 739, 1170, 1200
Bsc4I	CCNNNNNNNGG	4 cut(s)	98, 1227, 1402, 1431
Bse1I	ACTGG	6 cut(s)	131, 367, 449, 647, 670, 995
Bse3DI	GCAATG	1 cut(s)	641
BseBI	CCWGG	3 cut(s)	300, 445, 1256
BseCI	ATCGAT	1 cut(s)	1385
BseDI	CCNNGG	2 cut(s)	299, 629
BseGI	GGATG	5 cut(s)	243, 283, 754, 1153, 1419
BseLI	CCNNNNNNNGG	4 cut(s)	98, 1227, 1402, 1431
BseMI	GCAATG	1 cut(s)	641
BseMII	CTCAG	1 cut(s)	638
BseNI	ACTGG	6 cut(s)	131, 367, 449, 647, 670, 995
BseRI	GAGGAG	5 cut(s)	590, 593, 930, 1173, 1414
BsgI	GTGCAG	1 cut(s)	1333
BshFI	GGCC	2 cut(s)	448, 1435
BshVI	ATCGAT	1 cut(s)	1385
BsiHKAI	GWGCWC	1 cut(s)	978
BsiSI	CCGG	3 cut(s)	93, 122, 1228
BslFI	GGGAC	2 cut(s)	75, 379
BslI	CCNNNNNNNGG	4 cut(s)	98, 1227, 1402, 1431
BsmAI	GTCTC	1 cut(s)	225
BsmFI	GGGAC	2 cut(s)	75, 379
BsnI	GGCC	2 cut(s)	448, 1435
Bsp1286I	GDGCHC	1 cut(s)	978
Bsp143I	GATC	9 cut(s)	37, 77, 157, 766, 862, 986, 1048, 1210, 1453
BspACI	CCGC	3 cut(s)	75, 800, 1144
BspANI	GGCC	2 cut(s)	448, 1435
BspCNI	CTCAG	1 cut(s)	637
BspDI	ATCGAT	1 cut(s)	1385
BspHI	TCATGA	1 cut(s)	769
BspLI	GGNNCC	2 cut(s)	22, 79
BspMAI	CTGCAG	1 cut(s)	1129
BspPI	GGATC	3 cut(s)	72, 85, 152
BspQI	GCTCTTC	1 cut(s)	54
BsrDI	GCAATG	1 cut(s)	641
BsrI	ACTGG	6 cut(s)	131, 367, 449, 647, 670, 995
BssECI	CCNNGG	2 cut(s)	299, 629
BssMI	GATC	9 cut(s)	37, 77, 157, 766, 862, 986, 1048, 1210, 1453
BssSI	CACGAG	1 cut(s)	1055
BssT1I	CCWWGG	1 cut(s)	629
Bst2BI	CACGAG	1 cut(s)	1055
Bst2UI	CCWGG	3 cut(s)	300, 445, 1256
Bst4CI	ACNGT	2 cut(s)	833, 931
Bst6I	CTCTTC	4 cut(s)	54, 700, 957, 1371
BstAPI	GCANNNNNTGC	4 cut(s)	797, 812, 1096, 1124
BstBAI	YACGTR	1 cut(s)	957
BstC8I	GCNNGC	1 cut(s)	326
BstDEI	CTNAG	2 cut(s)	624, 735
BstF5I	GGATG	5 cut(s)	243, 283, 754, 1153, 1419
BstHHI	GCGC	1 cut(s)	1261
BstKTI	GATC	9 cut(s)	40, 80, 160, 769, 865, 989, 1051, 1213, 1456
BstMAI	GTCTC	1 cut(s)	225
BstMBI	GATC	9 cut(s)	37, 77, 157, 766, 862, 986, 1048, 1210, 1453
BstNI	CCWGG	3 cut(s)	300, 445, 1256
BstSCI	CCNGG	5 cut(s)	91, 298, 443, 1226, 1254
BstSFI	CTRYAG	2 cut(s)	829, 1125
BstX2I	RGATCY	1 cut(s)	77
BstYI	RGATCY	1 cut(s)	77
Bsu15I	ATCGAT	1 cut(s)	1385
BsuRI	GGCC	2 cut(s)	448, 1435
BsuTUI	ATCGAT	1 cut(s)	1385
BtsCI	GGATG	5 cut(s)	243, 283, 754, 1153, 1419
BtsI	GCAGTG	2 cut(s)	92, 685
BtsIMutI	CAGTG	3 cut(s)	92, 685, 838
Cac8I	GCNNGC	1 cut(s)	326
CaiI	CAGNNNCTG	3 cut(s)	797, 812, 1096
CciI	TCATGA	1 cut(s)	769
CfoI	GCGC	1 cut(s)	1261
Cfr13I	GGNCC	1 cut(s)	21
ClaI	ATCGAT	1 cut(s)	1385
Csp6I	GTAC	4 cut(s)	12, 992, 998, 1420
CviAII	CATG	6 cut(s)	454, 572, 770, 1034, 1073, 1460
CviQI	GTAC	4 cut(s)	12, 992, 998, 1420
DdeI	CTNAG	2 cut(s)	624, 735
DpnI	GATC	9 cut(s)	39, 79, 159, 768, 864, 988, 1050, 1212, 1455
DpnII	GATC	9 cut(s)	37, 77, 157, 766, 862, 986, 1048, 1210, 1453
Eam1104I	CTCTTC	4 cut(s)	54, 700, 957, 1371
EarI	CTCTTC	4 cut(s)	54, 700, 957, 1371
Eco130I	CCWWGG	1 cut(s)	629
Eco32I	GATATC	1 cut(s)	1369
Eco47I	GGWCC	1 cut(s)	21
Eco57I	CTGAAG	2 cut(s)	853, 868
Eco72I	CACGTG	1 cut(s)	957
EcoRI	GAATTC	1 cut(s)	239
EcoRII	CCWGG	3 cut(s)	298, 443, 1254
EcoRV	GATATC	1 cut(s)	1369
EcoT14I	CCWWGG	1 cut(s)	629
ErhI	CCWWGG	1 cut(s)	629
FaeI	CATG	6 cut(s)	457, 575, 773, 1037, 1076, 1463
FalI	AAGNNNNNCTT	2 cut(s)	579, 611
FaqI	GGGAC	2 cut(s)	75, 379
FatI	CATG	6 cut(s)	453, 571, 769, 1033, 1072, 1459
FbaI	TGATCA	2 cut(s)	1048, 1210
FblI	GTMKAC	1 cut(s)	136
FokI	GGATG	5 cut(s)	230, 270, 741, 1140, 1426
FspBI	CTAG	3 cut(s)	491, 1040, 1311
GlaI	GCGC	1 cut(s)	1260
HaeIII	GGCC	2 cut(s)	448, 1435
HapII	CCGG	3 cut(s)	93, 122, 1228
HhaI	GCGC	1 cut(s)	1261
Hin1II	CATG	6 cut(s)	457, 575, 773, 1037, 1076, 1463
Hin6I	GCGC	1 cut(s)	1259
HinP1I	GCGC	1 cut(s)	1259
HindIII	AAGCTT	2 cut(s)	53, 892
HinfI	GANTC	3 cut(s)	622, 1217, 1379
HpaII	CCGG	3 cut(s)	93, 122, 1228
HphI	GGTGA	5 cut(s)	215, 469, 556, 680, 1004
Hpy166II	GTNNAC	2 cut(s)	101, 137
Hpy188I	TCNGA	8 cut(s)	29, 37, 69, 621, 649, 862, 887, 1048
Hpy188III	TCNNGA	6 cut(s)	287, 770, 1055, 1208, 1214, 1400
Hpy8I	GTNNAC	2 cut(s)	101, 137
HpyCH4III	ACNGT	2 cut(s)	833, 931
HpyCH4IV	ACGT	1 cut(s)	956
HpyF3I	CTNAG	2 cut(s)	624, 735
HpySE526I	ACGT	1 cut(s)	956
Hsp92II	CATG	6 cut(s)	457, 575, 773, 1037, 1076, 1463
HspAI	GCGC	1 cut(s)	1259
Ksp22I	TGATCA	2 cut(s)	1048, 1210
Kzo9I	GATC	9 cut(s)	37, 77, 157, 766, 862, 986, 1048, 1210, 1453
LguI	GCTCTTC	1 cut(s)	54
LmnI	GCTCC	3 cut(s)	820, 1242, 1307
LweI	GCATC	8 cut(s)	37, 73, 537, 1108, 1224, 1303, 1328, 1377
MaeI	CTAG	3 cut(s)	491, 1040, 1311
MaeII	ACGT	1 cut(s)	956
MaeIII	GTNAC	3 cut(s)	275, 304, 398
MalI	GATC	9 cut(s)	39, 79, 159, 768, 864, 988, 1050, 1212, 1455
MboI	GATC	9 cut(s)	37, 77, 157, 766, 862, 986, 1048, 1210, 1453
MboII	GAAGA	8 cut(s)	71, 275, 325, 390, 717, 723, 974, 1388
MfeI	CAATTG	1 cut(s)	252
MflI	RGATCY	1 cut(s)	77
MhlI	GDGCHC	1 cut(s)	978
MluCI	AATT	9 cut(s)	152, 177, 239, 252, 270, 486, 538, 1113, 1249
MlyI	GAGTC	2 cut(s)	616, 1388
MmeI	TCCRAC	1 cut(s)	185
MroXI	GAANNNNTTC	1 cut(s)	294
MspA1I	CMGCKG	3 cut(s)	797, 809, 1124
MspI	CCGG	3 cut(s)	93, 122, 1228
MspR9I	CCNGG	5 cut(s)	93, 300, 445, 1228, 1256
MunI	CAATTG	1 cut(s)	252
MvaI	CCWGG	3 cut(s)	300, 445, 1256
NciI	CCSGG	2 cut(s)	93, 1228
NdeII	GATC	9 cut(s)	37, 77, 157, 766, 862, 986, 1048, 1210, 1453
NlaIII	CATG	6 cut(s)	457, 575, 773, 1037, 1076, 1463
NlaIV	GGNNCC	2 cut(s)	22, 79
NmuCI	GTSAC	2 cut(s)	304, 398
PagI	TCATGA	1 cut(s)	769
PciSI	GCTCTTC	1 cut(s)	54
PdmI	GAANNNNTTC	1 cut(s)	294
PfeI	GAWTC	1 cut(s)	1217
PleI	GAGTC	2 cut(s)	616, 1387
PmaCI	CACGTG	1 cut(s)	957
PmlI	CACGTG	1 cut(s)	957
PpsI	GAGTC	2 cut(s)	616, 1387
Ppu21I	YACGTR	1 cut(s)	957
Psp6I	CCWGG	3 cut(s)	298, 443, 1254
PspCI	CACGTG	1 cut(s)	957
PspGI	CCWGG	3 cut(s)	298, 443, 1254
PspN4I	GGNNCC	2 cut(s)	22, 79
PspPI	GGNCC	1 cut(s)	21
PstI	CTGCAG	1 cut(s)	1129
PstNI	CAGNNNCTG	3 cut(s)	797, 812, 1096
PsuI	RGATCY	1 cut(s)	77
PvuII	CAGCTG	3 cut(s)	797, 809, 1124
RsaI	GTAC	4 cut(s)	13, 993, 999, 1421
RsaNI	GTAC	4 cut(s)	12, 992, 998, 1420
SapI	GCTCTTC	1 cut(s)	54
Sau3AI	GATC	9 cut(s)	37, 77, 157, 766, 862, 986, 1048, 1210, 1453
Sau96I	GGNCC	1 cut(s)	21
ScaI	AGTACT	2 cut(s)	13, 1421
SchI	GAGTC	2 cut(s)	616, 1388
ScrFI	CCNGG	5 cut(s)	93, 300, 445, 1228, 1256
SduI	GDGCHC	1 cut(s)	978
SfaNI	GCATC	8 cut(s)	37, 73, 537, 1108, 1224, 1303, 1328, 1377
SfcI	CTRYAG	2 cut(s)	829, 1125
SinI	GGWCC	1 cut(s)	21
SmlI	CTYRAG	3 cut(s)	319, 823, 1398
SmoI	CTYRAG	3 cut(s)	319, 823, 1398
SpeI	ACTAGT	1 cut(s)	1039
Sse9I	AATT	9 cut(s)	152, 177, 239, 252, 270, 486, 538, 1113, 1249
SsiI	CCGC	3 cut(s)	75, 800, 1144
SspI	AATATT	1 cut(s)	744
SspMI	CTAG	3 cut(s)	491, 1040, 1311
StyD4I	CCNGG	5 cut(s)	91, 298, 443, 1226, 1254
StyI	CCWWGG	1 cut(s)	629
TaaI	ACNGT	2 cut(s)	833, 931
TaiI	ACGT	1 cut(s)	959
TaqI	TCGA	2 cut(s)	111, 1385
TasI	AATT	9 cut(s)	152, 177, 239, 252, 270, 486, 538, 1113, 1249
TatI	WGTACW	3 cut(s)	11, 997, 1419
TauI	GCSGC	1 cut(s)	1146
TfiI	GAWTC	1 cut(s)	1217
TscAI	CASTG	3 cut(s)	92, 685, 838
TseFI	GTSAC	2 cut(s)	304, 398
Tsp45I	GTSAC	2 cut(s)	304, 398
TspDTI	ATGAA	7 cut(s)	17, 232, 252, 836, 1050, 1089, 1430
TspRI	CASTG	3 cut(s)	92, 685, 838
VpaK11BI	GGWCC	1 cut(s)	21
XapI	RAATTY	5 cut(s)	152, 239, 270, 486, 1249
XcmI	CCANNNNNNNNNTGG	2 cut(s)	451, 560
XmiI	GTMKAC	1 cut(s)	136
XmnI	GAANNNNTTC	1 cut(s)	294
XspI	CTAG	3 cut(s)	491, 1040, 1311
ZrmI	AGTACT	2 cut(s)	13, 1421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.