FvH4_1g08020

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006526
Physical Location & Seq
Reverse (-)
4241091 .. 4244981
3891 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g08020.t2

Sequence Viewer

Length: 1251 bp
ATGAACAAAGGAGGCTTGGGGTCTAAGCTCAAGCTTACTCTTCCTCCTCCTGATGATATCAAGAAGTTCCTGACTCAAAGTGGTACCTTTATGGATGGGGATCTGCTGGTCAATAGGGAAGGAGTTCGGATCGTCTCTAATAGAGAAGTTGAAGTTCCAGCCCCTATTCAAGCAGCAGACAATCAGATGATTTTAGCAGACCTCTACGTGGTTAAAGTCATTGGTAAAGGGAATGGTGGGGTTGTGCAATTGGTTCAGCACAAATGGACTGGCCAGTTTTTTGCATTGAAGGTAATCCAAATGAATATTCAGGAGTCTATCCGCAAGCAGATTGCACAAGAATTGAAAATTAATCAATCATCACAATGTCCATTTATTGTTGTTTGTTACCGGTCCTTCTATGATAATGGTTGCATTTCAATTATCCTAGAGTACATGGATGGTGGGTCTTTAGCAGATCTTCTGAGAAAAGTCAGATCAATTCCAGAGCCATATATTTCTGCCATGTGTAAGCAGGTGCTGCGTGGTTTGTTGTATCTTCATCATGAAAAACACATTATTCACAGGGACTTCAAACCCTCAAACTTGTTAATAAATCACAGAGGAGAAATTAAGATAACTGACTTTGGCGTGAGTGCAATAAAGGCAAACACCTCTGAACAGGCAAATACTTTTGTTGGCACCTACAACTATATGTCGCCAGAGAGAATACAAGGTCACAGCTATAGCTACAAAAGTGACATTTGGAGCTTGGGCTTAGTACTGCTGGAGTGTGCGACAGGTAAATTCCCATATACACCACCAGATCAAAGTGAAGGATGGGAAAACTTTTTTGAGCTTATGAGTGCTGTTGTTGACTTGCCGCCGCCTTGTGCACCTTCCGATCAGTTTTCTCCAGAGTTCTGCTCATTTATTTCTGCATGTGTTCAGAAAGATCCTAAGAAAAGACTGTCAGCACATGATCTCCTAGCGCATCCTTTCATCAGCATGTATGATGACTTGAATATTGACCTGTCCTCTTACTTCACTGAAGCAGGATCTCCACTTGCAAACCTTCTAAATCTATCTGGATGGGGACTGCCTAGGTATTGCGGACGAGAAGGTAATACCGGAAAATCAAAAGCTCTGCTGAAGGCTGTTGGTCACTTTTTTTCAGTATTAGTTCCCTTGGTAGTGAGAGGGCTATTCTGTAATGGACAAAATATTGTTAATGCTTGGAATGTTGGAATTTTTGACTACTTAGCTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000187 GO:0001932 GO:0001934 GO:0002376 GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009628 GO:0009631 GO:0009651 GO:0009814 GO:0009893 GO:0009914 GO:0009966 GO:0009967 GO:0009987 GO:0010051 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0098542 GO:0140096 GO:1901564 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

417

Amino Acids

46.33

Weight (kDa)

7.56

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 70 - 328 7.6e-72 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 71 - 323 1.3e-37 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 172 - 279 2.5e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 505
Acc36I ACCTGC 1 cut(s) 505
Acc65I GGTACC 1 cut(s) 83
AccB1I GGYRCC 2 cut(s) 83, 680
AciI CCGC 4 cut(s) 322, 863, 866, 1092
AclWI GGATC 4 cut(s) 108, 137, 929, 1045
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 2 cut(s) 785, 1227
AcuI CTGAAG 2 cut(s) 1050, 1151
AfaI GTAC 3 cut(s) 85, 434, 762
AfiI CCNNNNNNNGG 1 cut(s) 208
AgeI ACCGGT 1 cut(s) 390
AgsI TTSAA 7 cut(s) 152, 170, 289, 346, 420, 574, 1003
AjuI GAANNNNNNNTTGG 2 cut(s) 291, 323
AluBI AGCT 8 cut(s) 28, 34, 723, 729, 750, 838, 1124, 1244
AluI AGCT 8 cut(s) 28, 34, 723, 729, 750, 838, 1124, 1244
Alw21I GWGCWC 1 cut(s) 877
Alw26I GTCTC 1 cut(s) 139
Alw44I GTGCAC 1 cut(s) 873
AlwI GGATC 4 cut(s) 108, 137, 929, 1045
AlwNI CAGNNNCTG 1 cut(s) 520
AoxI GGCC 1 cut(s) 271
ApaLI GTGCAC 1 cut(s) 873
ApeKI GCWGC 2 cut(s) 173, 520
ApoI RAATTY 2 cut(s) 785, 1227
AseI ATTAAT 1 cut(s) 351
AsiGI ACCGGT 1 cut(s) 390
Asp700I GAANNNNTTC 1 cut(s) 123
Asp718I GGTACC 1 cut(s) 83
AspA2I CCTAGG 1 cut(s) 1082
AspLEI GCGC 1 cut(s) 973
AspS9I GGNCC 1 cut(s) 393
AvaII GGWCC 1 cut(s) 393
AvrII CCTAGG 1 cut(s) 1082
BaeGI GKGCMC 1 cut(s) 877
BalI TGGCCA 1 cut(s) 273
BanI GGYRCC 2 cut(s) 83, 680
Bbv12I GWGCWC 1 cut(s) 877
BbvI GCAGC 2 cut(s) 185, 507
BccI CCATC 4 cut(s) 89, 434, 813, 1065
BcoDI GTCTC 1 cut(s) 139
BfaI CTAG 4 cut(s) 428, 968, 1083, 1249
BfmI CTRYAG 1 cut(s) 724
BfuAI ACCTGC 1 cut(s) 505
BglII AGATCT 1 cut(s) 457
BisI GCNGC 4 cut(s) 174, 521, 863, 866
BlnI CCTAGG 1 cut(s) 1082
BlsI GCNGC 4 cut(s) 175, 522, 864, 867
BmcAI AGTACT 1 cut(s) 762
Bme18I GGWCC 1 cut(s) 393
BmgT120I GGNCC 1 cut(s) 393
BmiI GGNNCC 2 cut(s) 85, 682
BmsI GCATC 1 cut(s) 982
BplI GAGNNNNNCTC 2 cut(s) 890, 922
BpmI CTGGAG 2 cut(s) 788, 879
BpuEI CTTGAG 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 208
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 1082, 1167
BsaWI WCCGGW 2 cut(s) 390, 1109
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 948, 978
Bsc4I CCNNNNNNNGG 1 cut(s) 208
Bse118I RCCGGY 1 cut(s) 390
Bse1I ACTGG 2 cut(s) 274, 274
Bse8I GATNNNNATC 1 cut(s) 99
BseDI CCNNGG 2 cut(s) 1082, 1167
BseGI GGATG 5 cut(s) 100, 445, 824, 973, 1076
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 1 cut(s) 208
BseMII CTCAG 1 cut(s) 455
BseNI ACTGG 2 cut(s) 274, 274
BseRI GAGGAG 2 cut(s) 36, 618
BseSI GKGCMC 1 cut(s) 877
BseXI GCAGC 2 cut(s) 185, 507
BshFI GGCC 1 cut(s) 273
BshNI GGYRCC 2 cut(s) 83, 680
BshTI ACCGGT 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 877
BsiSI CCGG 2 cut(s) 391, 1110
BslFI GGGAC 2 cut(s) 581, 1089
BslI CCNNNNNNNGG 1 cut(s) 208
BsmAI GTCTC 1 cut(s) 139
BsmBI CGTCTC 1 cut(s) 139
BsmFI GGGAC 2 cut(s) 581, 1089
BsnI GGCC 1 cut(s) 273
Bsp1286I GDGCHC 1 cut(s) 877
Bsp143I GATC 9 cut(s) 100, 129, 457, 476, 805, 883, 934, 961, 1037
BspACI CCGC 4 cut(s) 322, 863, 866, 1092
BspANI GGCC 1 cut(s) 273
BspCNI CTCAG 1 cut(s) 456
BspHI TCATGA 1 cut(s) 544
BspLI GGNNCC 2 cut(s) 85, 682
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 4 cut(s) 108, 137, 929, 1045
BspT107I GGYRCC 2 cut(s) 83, 680
BsrFI RCCGGY 1 cut(s) 390
BsrI ACTGG 2 cut(s) 274, 274
BssAI RCCGGY 1 cut(s) 390
BssECI CCNNGG 2 cut(s) 1082, 1167
BssMI GATC 9 cut(s) 100, 129, 457, 476, 805, 883, 934, 961, 1037
BssT1I CCWWGG 2 cut(s) 1082, 1167
Bst4CI ACNGT 1 cut(s) 951
Bst6I CTCTTC 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 520
BstBAI YACGTR 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 326
BstDEI CTNAG 5 cut(s) 24, 464, 757, 939, 1240
BstF5I GGATG 5 cut(s) 100, 445, 824, 973, 1076
BstHHI GCGC 1 cut(s) 973
BstKTI GATC 9 cut(s) 103, 132, 460, 479, 808, 886, 937, 964, 1040
BstMAI GTCTC 1 cut(s) 139
BstMBI GATC 9 cut(s) 100, 129, 457, 476, 805, 883, 934, 961, 1037
BstMWI GCNNNNNNNGC 2 cut(s) 520, 644
BstNSI RCATGY 2 cut(s) 924, 991
BstSFI CTRYAG 1 cut(s) 724
BstSLI GKGCMC 1 cut(s) 877
BstV1I GCAGC 2 cut(s) 185, 507
BstX2I RGATCY 4 cut(s) 100, 457, 934, 1037
BstYI RGATCY 4 cut(s) 100, 457, 934, 1037
BsuRI GGCC 1 cut(s) 273
BtsCI GGATG 5 cut(s) 100, 445, 824, 973, 1076
BtsIMutI CAGTG 1 cut(s) 1026
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 1 cut(s) 326
CaiI CAGNNNCTG 1 cut(s) 520
CciI TCATGA 1 cut(s) 544
CfoI GCGC 1 cut(s) 973
Cfr10I RCCGGY 1 cut(s) 390
Cfr13I GGNCC 1 cut(s) 393
Csp6I GTAC 3 cut(s) 84, 433, 761
CspAI ACCGGT 1 cut(s) 390
CviAII CATG 6 cut(s) 436, 505, 545, 921, 959, 988
CviQI GTAC 3 cut(s) 84, 433, 761
DdeI CTNAG 5 cut(s) 24, 464, 757, 939, 1240
DpnI GATC 9 cut(s) 102, 131, 459, 478, 807, 885, 936, 963, 1039
DpnII GATC 9 cut(s) 100, 129, 457, 476, 805, 883, 934, 961, 1037
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco130I CCWWGG 2 cut(s) 1082, 1167
Eco32I GATATC 1 cut(s) 58
Eco47I GGWCC 1 cut(s) 393
Eco57I CTGAAG 2 cut(s) 1050, 1151
EcoRV GATATC 1 cut(s) 58
EcoT14I CCWWGG 2 cut(s) 1082, 1167
ErhI CCWWGG 2 cut(s) 1082, 1167
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 6 cut(s) 439, 508, 548, 924, 962, 991
FaqI GGGAC 2 cut(s) 581, 1089
FatI CATG 6 cut(s) 435, 504, 544, 920, 958, 987
Fnu4HI GCNGC 4 cut(s) 174, 521, 863, 866
FokI GGATG 5 cut(s) 107, 452, 831, 960, 1083
Fsp4HI GCNGC 4 cut(s) 174, 521, 863, 866
FspBI CTAG 4 cut(s) 428, 968, 1083, 1249
GlaI GCGC 1 cut(s) 972
GluI GCNGC 4 cut(s) 174, 521, 863, 866
GsuI CTGGAG 2 cut(s) 788, 879
HaeIII GGCC 1 cut(s) 273
HapII CCGG 2 cut(s) 391, 1110
HhaI GCGC 1 cut(s) 973
Hin1II CATG 6 cut(s) 439, 508, 548, 924, 962, 991
Hin6I GCGC 1 cut(s) 971
HinP1I GCGC 1 cut(s) 971
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 2 cut(s) 73, 314
HpaII CCGG 2 cut(s) 391, 1110
Hpy166II GTNNAC 2 cut(s) 856, 875
Hpy188I TCNGA 7 cut(s) 129, 186, 465, 476, 658, 883, 930
Hpy188III TCNNGA 8 cut(s) 50, 61, 70, 311, 485, 545, 896, 1068
Hpy8I GTNNAC 2 cut(s) 856, 875
HpyAV CCTTC 8 cut(s) 113, 283, 406, 809, 888, 1064, 1094, 1126
HpyCH4III ACNGT 1 cut(s) 951
HpyCH4IV ACGT 1 cut(s) 207
HpyCH4V TGCA 8 cut(s) 247, 284, 335, 414, 638, 875, 920, 1049
HpyF10VI GCNNNNNNNGC 2 cut(s) 520, 644
HpyF3I CTNAG 5 cut(s) 24, 464, 757, 939, 1240
HpySE526I ACGT 1 cut(s) 207
Hsp92II CATG 6 cut(s) 439, 508, 548, 924, 962, 991
HspAI GCGC 1 cut(s) 971
KpnI GGTACC 1 cut(s) 87
Kzo9I GATC 9 cut(s) 100, 129, 457, 476, 805, 883, 934, 961, 1037
LmnI GCTCC 1 cut(s) 747
Lsp1109I GCAGC 2 cut(s) 185, 507
LweI GCATC 1 cut(s) 982
MaeI CTAG 4 cut(s) 428, 968, 1083, 1249
MaeII ACGT 1 cut(s) 207
MaeIII GTNAC 4 cut(s) 386, 716, 737, 1142
MalI GATC 9 cut(s) 102, 131, 459, 478, 807, 885, 936, 963, 1039
MboI GATC 9 cut(s) 100, 129, 457, 476, 805, 883, 934, 961, 1037
MboII GAAGA 3 cut(s) 32, 452, 530
MfeI CAATTG 1 cut(s) 248
MflI RGATCY 4 cut(s) 100, 457, 934, 1037
MhlI GDGCHC 1 cut(s) 877
MlsI TGGCCA 1 cut(s) 273
MluCI AATT 8 cut(s) 248, 341, 348, 420, 480, 609, 785, 1227
MluNI TGGCCA 1 cut(s) 273
MlyI GAGTC 2 cut(s) 67, 323
MmeI TCCRAC 1 cut(s) 1204
MnlI CCTC 9 cut(s) 5, 54, 57, 212, 589, 596, 664, 1027, 1172
Mox20I TGGCCA 1 cut(s) 273
MroXI GAANNNNTTC 1 cut(s) 123
MscI TGGCCA 1 cut(s) 273
MseI TTAA 5 cut(s) 213, 351, 590, 612, 1209
MslI CAYNNNNRTG 2 cut(s) 364, 986
Msp20I TGGCCA 1 cut(s) 273
MspI CCGG 2 cut(s) 391, 1110
MunI CAATTG 1 cut(s) 248
MwoI GCNNNNNNNGC 2 cut(s) 520, 644
NdeII GATC 9 cut(s) 100, 129, 457, 476, 805, 883, 934, 961, 1037
NlaIII CATG 6 cut(s) 439, 508, 548, 924, 962, 991
NlaIV GGNNCC 2 cut(s) 85, 682
NmuCI GTSAC 3 cut(s) 716, 737, 1142
NspI RCATGY 2 cut(s) 924, 991
PagI TCATGA 1 cut(s) 544
PaqCI CACCTGC 1 cut(s) 505
PdmI GAANNNNTTC 1 cut(s) 123
PinAI ACCGGT 1 cut(s) 390
PkrI GCNGC 4 cut(s) 175, 522, 864, 867
PleI GAGTC 2 cut(s) 67, 322
PpsI GAGTC 2 cut(s) 67, 322
Ppu21I YACGTR 1 cut(s) 208
PshBI ATTAAT 1 cut(s) 351
PspN4I GGNNCC 2 cut(s) 85, 682
PspPI GGNCC 1 cut(s) 393
PstNI CAGNNNCTG 1 cut(s) 520
PsuI RGATCY 4 cut(s) 100, 457, 934, 1037
RsaI GTAC 3 cut(s) 85, 434, 762
RsaNI GTAC 3 cut(s) 84, 433, 761
RseI CAYNNNNRTG 2 cut(s) 364, 986
SaqAI TTAA 5 cut(s) 213, 351, 590, 612, 1209
SatI GCNGC 4 cut(s) 174, 521, 863, 866
Sau3AI GATC 9 cut(s) 100, 129, 457, 476, 805, 883, 934, 961, 1037
Sau96I GGNCC 1 cut(s) 393
ScaI AGTACT 1 cut(s) 762
SchI GAGTC 2 cut(s) 67, 323
SduI GDGCHC 1 cut(s) 877
SfaNI GCATC 1 cut(s) 982
SfcI CTRYAG 1 cut(s) 724
SinI GGWCC 1 cut(s) 393
SmiMI CAYNNNNRTG 2 cut(s) 364, 986
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 8 cut(s) 248, 341, 348, 420, 480, 609, 785, 1227
SsiI CCGC 4 cut(s) 322, 863, 866, 1092
SspI AATATT 3 cut(s) 307, 1006, 1204
SspMI CTAG 4 cut(s) 428, 968, 1083, 1249
StyI CCWWGG 2 cut(s) 1082, 1167
TaaI ACNGT 1 cut(s) 951
TaiI ACGT 1 cut(s) 210
TasI AATT 8 cut(s) 248, 341, 348, 420, 480, 609, 785, 1227
TatI WGTACW 2 cut(s) 432, 760
TauI GCSGC 2 cut(s) 865, 868
Tru1I TTAA 5 cut(s) 213, 351, 590, 612, 1209
Tru9I TTAA 5 cut(s) 213, 351, 590, 612, 1209
TscAI CASTG 1 cut(s) 1033
TseFI GTSAC 3 cut(s) 716, 737, 1142
TseI GCWGC 2 cut(s) 173, 520
Tsp45I GTSAC 3 cut(s) 716, 737, 1142
TspDTI ATGAA 5 cut(s) 17, 317, 530, 561, 970
TspRI CASTG 1 cut(s) 1033
VneI GTGCAC 1 cut(s) 873
VpaK11BI GGWCC 1 cut(s) 393
VspI ATTAAT 1 cut(s) 351
XapI RAATTY 2 cut(s) 785, 1227
XceI RCATGY 2 cut(s) 924, 991
XmaJI CCTAGG 1 cut(s) 1082
XmnI GAANNNNTTC 1 cut(s) 123
XspI CTAG 4 cut(s) 428, 968, 1083, 1249
ZrmI AGTACT 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.