Rh2DG087400

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006526
Physical Location & Seq
Reverse (-)
6779350 .. 6783040
3691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG087400.1

Sequence Viewer

Length: 933 bp
ATGAACAAGGGAAGCTTAGGCTCTAAGCTCAAGCTTAGTCTTCCTCCTCCTGATGAAGATTCTATCAAGAAGTTCCTGACTCAAAGTGGCACCTTTATGGATGGGGATCTGCTGGTGAATAGGGAAGGGGTTCGGATCGTCTCTCATAGAGAAGTTGAAGTTCCAGCCCCTATTCAAGCAGCAGACAATCAGATGATTTTAGCAGATCTCTACGTGGTTAAAGTCATTGGTAAAGGAAATGGTGGGGTTGTGCAATTGGTTCAGCACAAATGGACTGGCCAGTTTTTTGCATTGAAGGTAATCCAAATGAATATTCAGGAGTCTATTCGCAAGCAGATTGCACAAGAATTGAAAATTAATCAATCATCACAATGTCCCTATATTGTTGTATGTTACCAGTCCTTCTATGATAATGGTTGCATTTCAATTATCCTAGAGTACATGGATGGTGGATCTTTAGCAGATCTTCTGAAAAAAGTCAGATCAATTCCAGAGCCATATATTTCTGCTATGTGTAAGCAGGTGCTGCGCGGTTTGTTGTATCTTCATCATGAAAAACACATAATTCACAGGGACTTCAAACCCTCGAACTTGTTAATAAATCATAGAGGAGAAATTAAGATAACTGACTTTGGTGTCAGTGCAATAAAGGCGAACACCTCTGAACAGGCAAATACTTTTGTTGGCACATACAACTATATGTCGCCAGAGAGAATACAAGGTAACAGCTATAGCTACAAAAGTGACATTTGGAGCTTGGGCTTAGTACTACTGGAGTGTGCAACAGGTAAATTCCCATATACACCACCAGATCAAAGTGAAGGATGGGAAAACTTTTTTGAGCTTATGAGTGCTGTTGTTGACTTCCCACCACCTTCTGCACCTTCCGATCAGTTTTCTCCAGAGTTCTGCTCATTTATTTCTGCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000187 GO:0001932 GO:0001934 GO:0002376 GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009628 GO:0009631 GO:0009651 GO:0009814 GO:0009893 GO:0009914 GO:0009966 GO:0009967 GO:0009987 GO:0010051 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0098542 GO:0140096 GO:1901564 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

310

Amino Acids

34.76

Weight (kDa)

6.18

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 72 - 308 3.8e-63 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 73 - 274 3.9e-35 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 143 - 215 5.9e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 174 - 282 1.6e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 511
AasI GACNNNNNNGTC 1 cut(s) 635
Acc36I ACCTGC 1 cut(s) 511
AccB1I GGYRCC 1 cut(s) 89
AccII CGCG 1 cut(s) 531
AciI CCGC 1 cut(s) 531
AclWI GGATC 3 cut(s) 114, 143, 460
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 1 cut(s) 791
AfaI GTAC 2 cut(s) 440, 768
AgsI TTSAA 6 cut(s) 158, 176, 295, 352, 426, 580
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AluBI AGCT 7 cut(s) 15, 28, 34, 729, 735, 756, 844
AluI AGCT 7 cut(s) 15, 28, 34, 729, 735, 756, 844
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 3 cut(s) 114, 143, 460
AlwNI CAGNNNCTG 1 cut(s) 526
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 2 cut(s) 179, 526
ApoI RAATTY 1 cut(s) 791
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 129
AspLEI GCGC 1 cut(s) 531
AsuHPI GGTGA 1 cut(s) 127
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 191, 513
BccI CCATC 3 cut(s) 95, 440, 819
BcoDI GTCTC 1 cut(s) 145
BfaI CTAG 1 cut(s) 434
BfmI CTRYAG 1 cut(s) 730
BfuAI ACCTGC 1 cut(s) 511
BglII AGATCT 2 cut(s) 205, 463
BisI GCNGC 2 cut(s) 180, 527
BlsI GCNGC 2 cut(s) 181, 528
BmcAI AGTACT 1 cut(s) 768
BmiI GGNNCC 1 cut(s) 91
BpiI GAAGAC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BpmI CTGGAG 2 cut(s) 794, 885
Bpu10I CCTNAGC 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 105
Bse1I ACTGG 4 cut(s) 280, 280, 397, 777
Bse8I GATNNNNATC 1 cut(s) 105
BseGI GGATG 3 cut(s) 106, 451, 830
BseJI GATNNNNATC 1 cut(s) 105
BseNI ACTGG 4 cut(s) 280, 280, 397, 777
BseRI GAGGAG 2 cut(s) 36, 624
BseXI GCAGC 2 cut(s) 191, 513
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 1 cut(s) 531
BshFI GGCC 1 cut(s) 279
BshNI GGYRCC 1 cut(s) 89
BslFI GGGAC 2 cut(s) 360, 587
BsmAI GTCTC 1 cut(s) 145
BsmBI CGTCTC 1 cut(s) 145
BsmFI GGGAC 2 cut(s) 360, 587
BsnI GGCC 1 cut(s) 279
Bsp143I GATC 8 cut(s) 106, 135, 205, 452, 463, 482, 811, 889
BspACI CCGC 1 cut(s) 531
BspANI GGCC 1 cut(s) 279
BspFNI CGCG 1 cut(s) 531
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 3 cut(s) 114, 143, 460
BspT107I GGYRCC 1 cut(s) 89
BsrI ACTGG 4 cut(s) 280, 280, 397, 777
BssMI GATC 8 cut(s) 106, 135, 205, 452, 463, 482, 811, 889
BstAPI GCANNNNNTGC 1 cut(s) 526
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 1 cut(s) 332
BstDEI CTNAG 4 cut(s) 16, 24, 35, 763
BstF5I GGATG 3 cut(s) 106, 451, 830
BstFNI CGCG 1 cut(s) 531
BstHHI GCGC 1 cut(s) 531
BstKTI GATC 8 cut(s) 109, 138, 208, 455, 466, 485, 814, 892
BstMAI GTCTC 1 cut(s) 145
BstMBI GATC 8 cut(s) 106, 135, 205, 452, 463, 482, 811, 889
BstMWI GCNNNNNNNGC 2 cut(s) 526, 650
BstSFI CTRYAG 1 cut(s) 730
BstUI CGCG 1 cut(s) 531
BstV1I GCAGC 2 cut(s) 191, 513
BstV2I GAAGAC 1 cut(s) 32
BstX2I RGATCY 4 cut(s) 106, 205, 452, 463
BstYI RGATCY 4 cut(s) 106, 205, 452, 463
BsuRI GGCC 1 cut(s) 279
BtsCI GGATG 3 cut(s) 106, 451, 830
BtsIMutI CAGTG 1 cut(s) 646
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 1 cut(s) 332
CaiI CAGNNNCTG 1 cut(s) 526
CciI TCATGA 1 cut(s) 550
CfoI GCGC 1 cut(s) 531
Csp6I GTAC 2 cut(s) 439, 767
CviAII CATG 3 cut(s) 442, 551, 927
CviQI GTAC 2 cut(s) 439, 767
DdeI CTNAG 4 cut(s) 16, 24, 35, 763
DpnI GATC 8 cut(s) 108, 137, 207, 454, 465, 484, 813, 891
DpnII GATC 8 cut(s) 106, 135, 205, 452, 463, 482, 811, 889
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
EaeI YGGCCR 1 cut(s) 277
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 3 cut(s) 445, 554, 930
FalI AAGNNNNNCTT 1 cut(s) 31
FaqI GGGAC 2 cut(s) 360, 587
FatI CATG 3 cut(s) 441, 550, 926
Fnu4HI GCNGC 2 cut(s) 180, 527
FokI GGATG 3 cut(s) 113, 458, 837
Fsp4HI GCNGC 2 cut(s) 180, 527
FspBI CTAG 1 cut(s) 434
GlaI GCGC 1 cut(s) 530
GluI GCNGC 2 cut(s) 180, 527
GsuI CTGGAG 2 cut(s) 794, 885
HaeIII GGCC 1 cut(s) 279
HhaI GCGC 1 cut(s) 531
Hin1II CATG 3 cut(s) 445, 554, 930
Hin6I GCGC 1 cut(s) 529
HinP1I GCGC 1 cut(s) 529
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HindIII AAGCTT 2 cut(s) 13, 32
HinfI GANTC 3 cut(s) 59, 79, 320
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 1 cut(s) 862
Hpy188I TCNGA 6 cut(s) 135, 192, 471, 482, 664, 889
Hpy188III TCNNGA 7 cut(s) 50, 67, 76, 317, 491, 551, 902
Hpy8I GTNNAC 1 cut(s) 862
HpyAV CCTTC 6 cut(s) 119, 289, 412, 815, 885, 894
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 8 cut(s) 253, 290, 341, 420, 644, 782, 881, 926
HpyF10VI GCNNNNNNNGC 2 cut(s) 526, 650
HpyF3I CTNAG 4 cut(s) 16, 24, 35, 763
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 3 cut(s) 445, 554, 930
HspAI GCGC 1 cut(s) 529
Kzo9I GATC 8 cut(s) 106, 135, 205, 452, 463, 482, 811, 889
LmnI GCTCC 1 cut(s) 753
Lsp1109I GCAGC 2 cut(s) 191, 513
MaeI CTAG 1 cut(s) 434
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 3 cut(s) 392, 722, 743
MalI GATC 8 cut(s) 108, 137, 207, 454, 465, 484, 813, 891
MboI GATC 8 cut(s) 106, 135, 205, 452, 463, 482, 811, 889
MboII GAAGA 4 cut(s) 32, 68, 458, 536
MfeI CAATTG 1 cut(s) 254
MflI RGATCY 4 cut(s) 106, 205, 452, 463
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 8 cut(s) 254, 347, 354, 426, 486, 564, 615, 791
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 2 cut(s) 73, 329
MnlI CCTC 5 cut(s) 54, 57, 595, 602, 670
Mox20I TGGCCA 1 cut(s) 279
MroXI GAANNNNTTC 1 cut(s) 129
MscI TGGCCA 1 cut(s) 279
MseI TTAA 4 cut(s) 219, 357, 596, 618
MslI CAYNNNNRTG 2 cut(s) 95, 370
Msp20I TGGCCA 1 cut(s) 279
MunI CAATTG 1 cut(s) 254
MvnI CGCG 1 cut(s) 531
MwoI GCNNNNNNNGC 2 cut(s) 526, 650
NdeII GATC 8 cut(s) 106, 135, 205, 452, 463, 482, 811, 889
NlaIII CATG 3 cut(s) 445, 554, 930
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 743
PagI TCATGA 1 cut(s) 550
PaqCI CACCTGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 129
PfeI GAWTC 1 cut(s) 59
PkrI GCNGC 2 cut(s) 181, 528
PleI GAGTC 2 cut(s) 73, 328
PpsI GAGTC 2 cut(s) 73, 328
Ppu21I YACGTR 1 cut(s) 214
PshBI ATTAAT 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 91
PstNI CAGNNNCTG 1 cut(s) 526
PsuI RGATCY 4 cut(s) 106, 205, 452, 463
RsaI GTAC 2 cut(s) 440, 768
RsaNI GTAC 2 cut(s) 439, 767
RseI CAYNNNNRTG 2 cut(s) 95, 370
SaqAI TTAA 4 cut(s) 219, 357, 596, 618
SatI GCNGC 2 cut(s) 180, 527
Sau3AI GATC 8 cut(s) 106, 135, 205, 452, 463, 482, 811, 889
ScaI AGTACT 1 cut(s) 768
SchI GAGTC 2 cut(s) 73, 329
SfcI CTRYAG 1 cut(s) 730
SmiMI CAYNNNNRTG 2 cut(s) 95, 370
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 8 cut(s) 254, 347, 354, 426, 486, 564, 615, 791
SsiI CCGC 1 cut(s) 531
SspI AATATT 1 cut(s) 313
SspMI CTAG 1 cut(s) 434
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 587
TasI AATT 8 cut(s) 254, 347, 354, 426, 486, 564, 615, 791
TatI WGTACW 2 cut(s) 438, 766
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 4 cut(s) 219, 357, 596, 618
Tru9I TTAA 4 cut(s) 219, 357, 596, 618
TscAI CASTG 1 cut(s) 646
TseFI GTSAC 1 cut(s) 743
TseI GCWGC 2 cut(s) 179, 526
Tsp45I GTSAC 1 cut(s) 743
TspDTI ATGAA 5 cut(s) 17, 69, 323, 536, 567
TspRI CASTG 1 cut(s) 646
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 1 cut(s) 791
XmnI GAANNNNTTC 1 cut(s) 129
XspI CTAG 1 cut(s) 434
ZrmI AGTACT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.