MD15G1212100.v1.1

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006526
Physical Location & Seq
Reverse (-)
17032191 .. 17035942
3752 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1212100.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGAAGAAAGGAAGCTTAGGATCCAACCTCAAGCTCACTCTCCCTCCTCCTGATGAAGTTTCCATCTCTAAGTTCTTGACACAAAGCGGTACGTTCATGGATGGGGATCTCCTGGTTAATAGAGAAGGGGTTCGGATCGTTTCTCATAGAGAAGTTGAAGTTCCACCACCTATTCAAGCATCAGACAACCAGATGATTTTAGCCGATCTCGACGCGATTAAAGTTATTGGCAAGGGAAATGGTGGGGTTGTGCAACTGGTTCAGCACAAATGGACTCATCAGTTTTTTGCATTAAAGGTAATTCAGATGAACATTGAAGAGTCTATCCGCAAGCAGATTGCACAGGAGTTGAAGATTAATCAATCGTCACAATGTCCAAACATTGTTGTATGTTACCAGTCCTTCTATGAAAATGGTACCATTTCAATCATCCTAGAGTACATGGATGGTGGGTCTTTGGCAGATCTTCTGAAGAAGGTCAAAACAATTCCAGAGTTTTATCTTGCTGCCATTTGTAAGCAGGTGCTGCAGGGTTTGTGTTACCTTCATCATGAAAAGCACATCATCCATCGGGACTTCAAACCCTCTAATTTGTTAATCAATCATAGGGGAGAAATTAAGATAACAGACTTTGGTGTGAGTGCAATAAAGGCAAGCACGTCCGAACAGGCAAATACATTTGTTGGCACATACAACTATATGTCGCCAGAGAGAATTGTCGGTAGCAATTATAGCTACAAAAGTGACATTTGGAGCTTGGGGTTAGTACTGCTGGAGTGTGCAACAGGTCAATTCCCATATACACCACCAGATCAAAGTGAAGCATGGGATAACTTTTTTGAGCTTATGAGTGCCATTGTTGAACAACCACCACCTTGTGCACCTTCTGATCAATTTTCTCCAGAGTTCTGCTCATTTATTTCTGCATGTGTACAGAAAGATCCTAAGGATAGATTGTCGGCTCAGGATCTCCTGAGACATCCTTTCATCAGCATGTACGATGACGTGCATATTGACCTTTCCTCTTACTTCACCGAAGCAGGATCTCCCCTTGCAACATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000187 GO:0001932 GO:0001934 GO:0002376 GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009628 GO:0009631 GO:0009651 GO:0009814 GO:0009893 GO:0009914 GO:0009966 GO:0009967 GO:0009987 GO:0010051 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0098542 GO:0140096 GO:1901564 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

355

Amino Acids

39.6

Weight (kDa)

5.48

Isoelectric Point (pI)

46.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 72 - 326 9.1e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 73 - 330 4.6e-72 Protein kinase domain
ABC1 PF03109 142 - 215 1.1e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 173 - 280 3.6e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 511
Acc36I ACCTGC 1 cut(s) 511
Acc65I GGTACC 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 416
AccII CGCG 1 cut(s) 215
AciI CCGC 2 cut(s) 87, 328
AclWI GGATC 7 cut(s) 15, 28, 114, 143, 935, 975, 1051
AcuI CTGAAG 1 cut(s) 491
AdeI CACNNNGTG 1 cut(s) 878
AfaI GTAC 6 cut(s) 91, 418, 440, 768, 933, 998
AgsI TTSAA 7 cut(s) 158, 176, 317, 352, 426, 580, 863
AjiI CACGTC 2 cut(s) 660, 1006
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 5 cut(s) 15, 34, 735, 756, 844
AluI AGCT 5 cut(s) 15, 34, 735, 756, 844
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 1 cut(s) 970
Alw44I GTGCAC 1 cut(s) 879
AlwI GGATC 7 cut(s) 15, 28, 114, 143, 935, 975, 1051
AlwNI CAGNNNCTG 1 cut(s) 526
ApaLI GTGCAC 1 cut(s) 879
ApeKI GCWGC 2 cut(s) 506, 526
AseI ATTAAT 1 cut(s) 357
Asp700I GAANNNNTTC 1 cut(s) 129
Asp718I GGTACC 1 cut(s) 416
AsuHPI GGTGA 1 cut(s) 1024
AxyI CCTNAGG 1 cut(s) 945
BaeGI GKGCMC 1 cut(s) 883
BamHI GGATCC 1 cut(s) 20
BanI GGYRCC 1 cut(s) 416
Bbv12I GWGCWC 1 cut(s) 883
BbvI GCAGC 2 cut(s) 493, 513
BccI CCATC 4 cut(s) 71, 95, 440, 576
BciT130I CCWGG 1 cut(s) 113
BclI TGATCA 1 cut(s) 889
BcoDI GTCTC 1 cut(s) 970
BfaI CTAG 1 cut(s) 434
BfmI CTRYAG 1 cut(s) 527
BfuAI ACCTGC 1 cut(s) 511
BglII AGATCT 1 cut(s) 463
BisI GCNGC 2 cut(s) 507, 527
BlsI GCNGC 2 cut(s) 508, 528
BmcAI AGTACT 1 cut(s) 768
Bme1390I CCNGG 1 cut(s) 113
BmgBI CACGTC 2 cut(s) 660, 1006
BmiI GGNNCC 2 cut(s) 22, 418
BmrFI CCNGG 1 cut(s) 113
BmsI GCATC 1 cut(s) 188
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BpmI CTGGAG 2 cut(s) 794, 885
Bpu10I CCTNAGC 2 cut(s) 16, 963
BpuEI CTTGAG 1 cut(s) 14
BsaBI GATNNNNATC 1 cut(s) 105
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bse1I ACTGG 2 cut(s) 261, 397
Bse21I CCTNAGG 1 cut(s) 945
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 1 cut(s) 113
BseGI GGATG 5 cut(s) 106, 429, 451, 564, 979
BseJI GATNNNNATC 1 cut(s) 105
BseMII CTCAG 2 cut(s) 965, 977
BseNI ACTGG 2 cut(s) 261, 397
BseRI GAGGAG 1 cut(s) 36
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 2 cut(s) 493, 513
Bsh1236I CGCG 1 cut(s) 215
BshNI GGYRCC 1 cut(s) 416
BsiHKAI GWGCWC 1 cut(s) 883
BslFI GGGAC 1 cut(s) 587
BsmAI GTCTC 1 cut(s) 970
BsmFI GGGAC 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 883
Bsp1407I TGTACA 1 cut(s) 931
BspACI CCGC 2 cut(s) 87, 328
BspCNI CTCAG 2 cut(s) 966, 976
BspFNI CGCG 1 cut(s) 215
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 2 cut(s) 22, 418
BspMAI CTGCAG 1 cut(s) 531
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 7 cut(s) 15, 28, 114, 143, 935, 975, 1051
BspT107I GGYRCC 1 cut(s) 416
BsrGI TGTACA 1 cut(s) 931
BsrI ACTGG 2 cut(s) 261, 397
Bst2UI CCWGG 1 cut(s) 113
Bst6I CTCTTC 1 cut(s) 312
BstAPI GCANNNNNTGC 1 cut(s) 526
BstAUI TGTACA 1 cut(s) 931
BstC8I GCNNGC 2 cut(s) 332, 655
BstDEI CTNAG 5 cut(s) 16, 69, 945, 963, 974
BstF5I GGATG 5 cut(s) 106, 429, 451, 564, 979
BstFNI CGCG 1 cut(s) 215
BstMAI GTCTC 1 cut(s) 970
BstMWI GCNNNNNNNGC 3 cut(s) 526, 650, 732
BstNI CCWGG 1 cut(s) 113
BstNSI RCATGY 2 cut(s) 930, 997
BstSCI CCNGG 1 cut(s) 111
BstSFI CTRYAG 1 cut(s) 527
BstSLI GKGCMC 1 cut(s) 883
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 2 cut(s) 493, 513
BstX2I RGATCY 6 cut(s) 20, 106, 463, 940, 967, 1043
BstYI RGATCY 6 cut(s) 20, 106, 463, 940, 967, 1043
Bsu36I CCTNAGG 1 cut(s) 945
BtrI CACGTC 2 cut(s) 660, 1006
BtsCI GGATG 5 cut(s) 106, 429, 451, 564, 979
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 2 cut(s) 332, 655
CaiI CAGNNNCTG 1 cut(s) 526
CciI TCATGA 1 cut(s) 550
CseI GACGC 1 cut(s) 221
Csp6I GTAC 6 cut(s) 90, 417, 439, 767, 932, 997
CviAII CATG 6 cut(s) 97, 442, 551, 825, 927, 994
CviJI RGCY 7 cut(s) 15, 34, 203, 735, 756, 844, 962
CviKI_1 RGCY 7 cut(s) 15, 34, 203, 735, 756, 844, 962
CviQI GTAC 6 cut(s) 90, 417, 439, 767, 932, 997
DdeI CTNAG 5 cut(s) 16, 69, 945, 963, 974
DraIII CACNNNGTG 1 cut(s) 878
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
Eco57I CTGAAG 1 cut(s) 491
Eco81I CCTNAGG 1 cut(s) 945
EcoRII CCWGG 1 cut(s) 111
FaeI CATG 6 cut(s) 100, 445, 554, 828, 930, 997
FaqI GGGAC 1 cut(s) 587
FatI CATG 6 cut(s) 96, 441, 550, 824, 926, 993
FbaI TGATCA 1 cut(s) 889
Fnu4HI GCNGC 2 cut(s) 507, 527
FokI GGATG 5 cut(s) 113, 416, 458, 551, 966
Fsp4HI GCNGC 2 cut(s) 507, 527
FspBI CTAG 1 cut(s) 434
GluI GCNGC 2 cut(s) 507, 527
GsuI CTGGAG 2 cut(s) 794, 885
HgaI GACGC 1 cut(s) 221
Hin1II CATG 6 cut(s) 100, 445, 554, 828, 930, 997
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 2 cut(s) 274, 320
HphI GGTGA 1 cut(s) 1024
Hpy166II GTNNAC 2 cut(s) 881, 932
Hpy188I TCNGA 6 cut(s) 135, 184, 306, 471, 664, 889
Hpy188III TCNNGA 9 cut(s) 50, 76, 209, 491, 551, 572, 902, 965, 973
Hpy8I GTNNAC 2 cut(s) 881, 932
Hpy99I CGWCG 1 cut(s) 215
HpyAV CCTTC 5 cut(s) 119, 412, 469, 554, 894
HpyCH4IV ACGT 3 cut(s) 92, 659, 1005
HpyF10VI GCNNNNNNNGC 3 cut(s) 526, 650, 732
HpyF3I CTNAG 5 cut(s) 16, 69, 945, 963, 974
HpySE526I ACGT 3 cut(s) 92, 659, 1005
Hsp92II CATG 6 cut(s) 100, 445, 554, 828, 930, 997
KpnI GGTACC 1 cut(s) 420
Ksp22I TGATCA 1 cut(s) 889
LmnI GCTCC 1 cut(s) 753
Lsp1109I GCAGC 2 cut(s) 493, 513
LweI GCATC 1 cut(s) 188
MaeI CTAG 1 cut(s) 434
MaeII ACGT 3 cut(s) 92, 659, 1005
MaeIII GTNAC 4 cut(s) 366, 392, 539, 743
MboII GAAGA 5 cut(s) 16, 329, 364, 458, 484
MflI RGATCY 6 cut(s) 20, 106, 463, 940, 967, 1043
MhlI GDGCHC 1 cut(s) 883
MluCI AATT 8 cut(s) 300, 486, 589, 615, 714, 727, 791, 893
MlyI GAGTC 2 cut(s) 268, 329
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 5 cut(s) 38, 54, 57, 595, 1033
MroXI GAANNNNTTC 1 cut(s) 129
MseI TTAA 7 cut(s) 117, 219, 293, 357, 596, 618, 1063
MslI CAYNNNNRTG 1 cut(s) 992
MspR9I CCNGG 1 cut(s) 113
MvaI CCWGG 1 cut(s) 113
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 3 cut(s) 526, 650, 732
NlaIII CATG 6 cut(s) 100, 445, 554, 828, 930, 997
NlaIV GGNNCC 2 cut(s) 22, 418
NmuCI GTSAC 2 cut(s) 366, 743
NspI RCATGY 2 cut(s) 930, 997
PagI TCATGA 1 cut(s) 550
PaqCI CACCTGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 129
PkrI GCNGC 2 cut(s) 508, 528
PleI GAGTC 2 cut(s) 268, 328
PpsI GAGTC 2 cut(s) 268, 328
PshBI ATTAAT 1 cut(s) 357
Psp6I CCWGG 1 cut(s) 111
PspGI CCWGG 1 cut(s) 111
PspN4I GGNNCC 2 cut(s) 22, 418
PstI CTGCAG 1 cut(s) 531
PstNI CAGNNNCTG 1 cut(s) 526
PsuI RGATCY 6 cut(s) 20, 106, 463, 940, 967, 1043
RsaI GTAC 6 cut(s) 91, 418, 440, 768, 933, 998
RsaNI GTAC 6 cut(s) 90, 417, 439, 767, 932, 997
RseI CAYNNNNRTG 1 cut(s) 992
SaqAI TTAA 7 cut(s) 117, 219, 293, 357, 596, 618, 1063
SatI GCNGC 2 cut(s) 507, 527
ScaI AGTACT 1 cut(s) 768
SchI GAGTC 2 cut(s) 268, 329
ScrFI CCNGG 1 cut(s) 113
SduI GDGCHC 1 cut(s) 883
SfaNI GCATC 1 cut(s) 188
SfcI CTRYAG 1 cut(s) 527
SmiMI CAYNNNNRTG 1 cut(s) 992
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
Sse9I AATT 8 cut(s) 300, 486, 589, 615, 714, 727, 791, 893
SsiI CCGC 2 cut(s) 87, 328
SspMI CTAG 1 cut(s) 434
StyD4I CCNGG 1 cut(s) 111
TaiI ACGT 3 cut(s) 95, 662, 1008
TaqI TCGA 1 cut(s) 210
TasI AATT 8 cut(s) 300, 486, 589, 615, 714, 727, 791, 893
TatI WGTACW 3 cut(s) 438, 766, 931
Tru1I TTAA 7 cut(s) 117, 219, 293, 357, 596, 618, 1063
Tru9I TTAA 7 cut(s) 117, 219, 293, 357, 596, 618, 1063
TseFI GTSAC 2 cut(s) 366, 743
TseI GCWGC 2 cut(s) 506, 526
Tsp45I GTSAC 2 cut(s) 366, 743
TspDTI ATGAA 8 cut(s) 17, 69, 85, 323, 423, 536, 567, 976
VneI GTGCAC 1 cut(s) 879
VspI ATTAAT 1 cut(s) 357
XceI RCATGY 2 cut(s) 930, 997
XmnI GAANNNNTTC 1 cut(s) 129
XspI CTAG 1 cut(s) 434
ZrmI AGTACT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.