Rh2AG088200

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0006526
Physical Location & Seq
Reverse (-)
7414361 .. 7418337
3977 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG088200.1

Sequence Viewer

Length: 759 bp
ATGAATATTCAGGAGTCTATTCGCAGGCAGATTGCACAAGAATTGAAAATTAATCAATCATCACAATGTCCCTATATTGTTGTATGTTACCAGTCCTTCTATGATAATGGTTGCATTTCAATTATCCTAGAGTACATGGATGGTGGATCTTTAGCAGATCTTCTGAAAAAAGTCAGATCAATTCCAGAGCCATATATTTCTGCTATGTGTAAGCAGGTGCTGCGCGGTTTGTTGTATCTTCATCATGAAAAACACATAATTCACAGGGACTTCAAACCCTCGAACTTGTTAATAAATCATAGAGGAGAAATTAAGATAACTGACTTTGGTGTCAGTGCAATAAAGGCAAACACCTCTGAACAGGCAAATACTTTTGTTGGCACATACAACTATATGTCGCCAGAGAGAATACAAGGTAACAGCTATAGCTACAAAAGTGACATTTGGAGCTTGGGCTTAGTACTACTGGAGTGTGCAACAGGTAAATTCCCATATACACCACCAGATCAAAGTGAAGGATGGGAAAACTTTTTTGAGCTTATGAGTGCTGTTGTTGACTTGCCACCACCTTCTGCACCTTCCGATCAGTTTTCTCCAGAGTTCTGCTCATTTATTTCTGCATGTGTACAGAAAGATCCTAAAAAGAGATTGTCAGCACATGATCTCCTAGTGCATCCTTTTATCAGCATGTACGACGACTTGAATATCGACCTTTCGTCTTACTTCACTGAAGCAGGATCTCCACTTGCAACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000187 GO:0001932 GO:0001934 GO:0002376 GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009628 GO:0009631 GO:0009651 GO:0009814 GO:0009893 GO:0009914 GO:0009966 GO:0009967 GO:0009987 GO:0010051 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0098542 GO:0140096 GO:1901564 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

252

Amino Acids

28.5

Weight (kDa)

5.48

Isoelectric Point (pI)

58.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 5 - 228 4.9e-63 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 5 - 222 1e-34 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 41 - 113 3.7e-06 ABC1 atypical kinase-like domain
Kinase-like PF14531 72 - 179 1e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 205
AasI GACNNNNNNGTC 1 cut(s) 329
Acc36I ACCTGC 1 cut(s) 205
AccII CGCG 1 cut(s) 225
AciI CCGC 1 cut(s) 225
AclWI GGATC 3 cut(s) 154, 629, 745
AcsI RAATTY 1 cut(s) 485
AcuI CTGAAG 1 cut(s) 750
AfaI GTAC 4 cut(s) 134, 462, 627, 692
AgsI TTSAA 4 cut(s) 46, 120, 274, 703
AhdI GACNNNNNGTC 1 cut(s) 715
AluBI AGCT 4 cut(s) 423, 429, 450, 538
AluI AGCT 4 cut(s) 423, 429, 450, 538
AlwI GGATC 3 cut(s) 154, 629, 745
AlwNI CAGNNNCTG 1 cut(s) 220
ApeKI GCWGC 1 cut(s) 220
ApoI RAATTY 1 cut(s) 485
AseI ATTAAT 1 cut(s) 51
AspLEI GCGC 1 cut(s) 225
BbvI GCAGC 1 cut(s) 207
BccI CCATC 2 cut(s) 134, 513
BfaI CTAG 2 cut(s) 128, 668
BfmI CTRYAG 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 205
BglII AGATCT 1 cut(s) 157
BisI GCNGC 1 cut(s) 221
BlsI GCNGC 1 cut(s) 222
BmcAI AGTACT 1 cut(s) 462
BmeRI GACNNNNNGTC 1 cut(s) 715
BmsI GCATC 1 cut(s) 682
BplI GAGNNNNNCTC 2 cut(s) 590, 622
BpmI CTGGAG 2 cut(s) 488, 579
BsaXI ACNNNNNCTCC 2 cut(s) 648, 678
Bse1I ACTGG 2 cut(s) 91, 471
BseGI GGATG 3 cut(s) 145, 524, 673
BseNI ACTGG 2 cut(s) 91, 471
BseRI GAGGAG 1 cut(s) 318
BseXI GCAGC 1 cut(s) 207
BsgI GTGCAG 1 cut(s) 558
Bsh1236I CGCG 1 cut(s) 225
BslFI GGGAC 2 cut(s) 54, 281
BsmFI GGGAC 2 cut(s) 54, 281
Bsp1407I TGTACA 1 cut(s) 625
Bsp143I GATC 8 cut(s) 146, 157, 176, 505, 583, 634, 661, 737
BspACI CCGC 1 cut(s) 225
BspFNI CGCG 1 cut(s) 225
BspHI TCATGA 1 cut(s) 244
BspMI ACCTGC 1 cut(s) 205
BspPI GGATC 3 cut(s) 154, 629, 745
BsrGI TGTACA 1 cut(s) 625
BsrI ACTGG 2 cut(s) 91, 471
BssMI GATC 8 cut(s) 146, 157, 176, 505, 583, 634, 661, 737
BstAPI GCANNNNNTGC 1 cut(s) 220
BstAUI TGTACA 1 cut(s) 625
BstC8I GCNNGC 1 cut(s) 26
BstDEI CTNAG 1 cut(s) 457
BstF5I GGATG 3 cut(s) 145, 524, 673
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 1 cut(s) 225
BstKTI GATC 8 cut(s) 149, 160, 179, 508, 586, 637, 664, 740
BstMBI GATC 8 cut(s) 146, 157, 176, 505, 583, 634, 661, 737
BstMWI GCNNNNNNNGC 2 cut(s) 220, 344
BstNSI RCATGY 2 cut(s) 624, 691
BstSFI CTRYAG 1 cut(s) 424
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 207
BstX2I RGATCY 4 cut(s) 146, 157, 634, 737
BstYI RGATCY 4 cut(s) 146, 157, 634, 737
BtsCI GGATG 3 cut(s) 145, 524, 673
BtsIMutI CAGTG 2 cut(s) 340, 726
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 26
CaiI CAGNNNCTG 1 cut(s) 220
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 225
Csp6I GTAC 4 cut(s) 133, 461, 626, 691
CviAII CATG 5 cut(s) 136, 245, 621, 659, 688
CviJI RGCY 6 cut(s) 190, 423, 429, 450, 456, 538
CviKI_1 RGCY 6 cut(s) 190, 423, 429, 450, 456, 538
CviQI GTAC 4 cut(s) 133, 461, 626, 691
DdeI CTNAG 1 cut(s) 457
DpnI GATC 8 cut(s) 148, 159, 178, 507, 585, 636, 663, 739
DpnII GATC 8 cut(s) 146, 157, 176, 505, 583, 634, 661, 737
DrdI GACNNNNNNGTC 1 cut(s) 329
DriI GACNNNNNGTC 1 cut(s) 715
DseDI GACNNNNNNGTC 1 cut(s) 329
Eam1105I GACNNNNNGTC 1 cut(s) 715
Eco57I CTGAAG 1 cut(s) 750
FaeI CATG 5 cut(s) 139, 248, 624, 662, 691
FaqI GGGAC 2 cut(s) 54, 281
FatI CATG 5 cut(s) 135, 244, 620, 658, 687
Fnu4HI GCNGC 1 cut(s) 221
FokI GGATG 3 cut(s) 152, 531, 660
Fsp4HI GCNGC 1 cut(s) 221
FspBI CTAG 2 cut(s) 128, 668
GlaI GCGC 1 cut(s) 224
GluI GCNGC 1 cut(s) 221
GsuI CTGGAG 2 cut(s) 488, 579
HhaI GCGC 1 cut(s) 225
Hin1II CATG 5 cut(s) 139, 248, 624, 662, 691
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 1 cut(s) 14
Hpy166II GTNNAC 2 cut(s) 556, 626
Hpy188I TCNGA 4 cut(s) 165, 176, 358, 583
Hpy188III TCNNGA 4 cut(s) 11, 185, 245, 596
Hpy8I GTNNAC 2 cut(s) 556, 626
Hpy99I CGWCG 1 cut(s) 698
HpyAV CCTTC 4 cut(s) 106, 509, 579, 588
HpyCH4V TGCA 8 cut(s) 35, 114, 338, 476, 575, 620, 673, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 220, 344
HpyF3I CTNAG 1 cut(s) 457
Hsp92II CATG 5 cut(s) 139, 248, 624, 662, 691
HspAI GCGC 1 cut(s) 223
Kzo9I GATC 8 cut(s) 146, 157, 176, 505, 583, 634, 661, 737
LmnI GCTCC 1 cut(s) 447
Lsp1109I GCAGC 1 cut(s) 207
LweI GCATC 1 cut(s) 682
MaeI CTAG 2 cut(s) 128, 668
MaeIII GTNAC 3 cut(s) 86, 416, 437
MalI GATC 8 cut(s) 148, 159, 178, 507, 585, 636, 663, 739
MboI GATC 8 cut(s) 146, 157, 176, 505, 583, 634, 661, 737
MboII GAAGA 2 cut(s) 152, 230
MflI RGATCY 4 cut(s) 146, 157, 634, 737
MluCI AATT 7 cut(s) 41, 48, 120, 180, 258, 309, 485
MlyI GAGTC 1 cut(s) 23
MnlI CCTC 3 cut(s) 289, 296, 364
MseI TTAA 4 cut(s) 51, 290, 312, 757
MslI CAYNNNNRTG 1 cut(s) 64
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 2 cut(s) 220, 344
NdeII GATC 8 cut(s) 146, 157, 176, 505, 583, 634, 661, 737
NlaIII CATG 5 cut(s) 139, 248, 624, 662, 691
NmuCI GTSAC 1 cut(s) 437
NspI RCATGY 2 cut(s) 624, 691
PagI TCATGA 1 cut(s) 244
PaqCI CACCTGC 1 cut(s) 205
PkrI GCNGC 1 cut(s) 222
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
PshBI ATTAAT 1 cut(s) 51
PstNI CAGNNNCTG 1 cut(s) 220
PsuI RGATCY 4 cut(s) 146, 157, 634, 737
RsaI GTAC 4 cut(s) 134, 462, 627, 692
RsaNI GTAC 4 cut(s) 133, 461, 626, 691
RseI CAYNNNNRTG 1 cut(s) 64
SaqAI TTAA 4 cut(s) 51, 290, 312, 757
SatI GCNGC 1 cut(s) 221
Sau3AI GATC 8 cut(s) 146, 157, 176, 505, 583, 634, 661, 737
ScaI AGTACT 1 cut(s) 462
SchI GAGTC 1 cut(s) 23
SfaNI GCATC 1 cut(s) 682
SfcI CTRYAG 1 cut(s) 424
SmiMI CAYNNNNRTG 1 cut(s) 64
Sse9I AATT 7 cut(s) 41, 48, 120, 180, 258, 309, 485
SsiI CCGC 1 cut(s) 225
SspI AATATT 1 cut(s) 7
SspMI CTAG 2 cut(s) 128, 668
TaqI TCGA 2 cut(s) 281, 708
TasI AATT 7 cut(s) 41, 48, 120, 180, 258, 309, 485
TatI WGTACW 3 cut(s) 132, 460, 625
Tru1I TTAA 4 cut(s) 51, 290, 312, 757
Tru9I TTAA 4 cut(s) 51, 290, 312, 757
TscAI CASTG 2 cut(s) 340, 733
TseFI GTSAC 1 cut(s) 437
TseI GCWGC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 437
TspDTI ATGAA 3 cut(s) 17, 230, 261
TspRI CASTG 2 cut(s) 340, 733
VspI ATTAAT 1 cut(s) 51
XapI RAATTY 1 cut(s) 485
XceI RCATGY 2 cut(s) 624, 691
XspI CTAG 2 cut(s) 128, 668
ZrmI AGTACT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.