MD02G1084700.v1.1

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006526
Physical Location & Seq
Reverse (-)
6664892 .. 6669059
4168 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1084700.v1.1.491

Sequence Viewer

Length: 1065 bp
ATGAAGACAGGAACCTTAGGACCCAACCTCAAGCTCACTCTCCCTCCTCCTGATGAAGTTTTCATCTCTAAGTTCTTGACTCAAAGCGGCACGTTTAAGGATGGCGATCTCCTGGTTAATAAACAAGGGGTTCGGATCGTTTCTCATAGAGAAGTTGAATTTCCGCCACCTATTCAAGCATCGGACAACCAGATGATTTTAGCCGACCTCGACTCGATTAAAGTTATTGGCAAGGGAACTGGTGGGGTTGTGCAATTGGTTCAGCACAAATGGACTCGCCAGTTTTTTGCATTGAAGGTAATTCAGATGAACATTGAAGAGTCTATCCGCAGGCAGATTGTACAGGAATTGAAAATTAATCAATCATCACAATGTCCAAACATTGTTGTATGTTACCAGTCCTTCTACGATAATGGTACCATTTCAATCATCCTAGAGTACATGGATGGTGGGTCTTTGGCAAATCTTCTGAAGAAGGTCAAAACGATTCCAGAGTTTTATCTTGCTGCCATTTGTAAGCAGGTGCTGCAGGGTTTGTGCTATCTTCATCATAAAAAGCACATCATCCATCGGGACTTTAAACCTTCCAATTTGTTAATCAATCATAGAGGAGAAGTTAAGATAACGGACTTTGGTGTGAGTGCAATAAAGGCAAGCACGTCCGAACAGGCAAATACTTTCATTGGCACATACAACTATATGTCGCCAGAGAGAATCGCTTGTCGCAATTATAGCTACAAAAGTGACATTTGGAGCCTGGGGTTAGTACTGCTGGAGTGTGCAACAGGTCAATTCCCATATGTACCACCAGATCAAAGTGAAGCATGGTGTAACTTTTTTGAACTTATGAGTGCCATCGTTGATGAACCAGCACCTTGTGCCCCTTCCGATCAATTTTCTCCAGAGTTCTGCTCATTTATTTCTGCATGTGTACAGAAAGATCCGAAGGATAGACCATCAGCTCAAGATCTCCTGAGACATCCTTTCATCAACATGTACGATGACTTGCATATTGACCTTTCCTCTTACTTCACCGAAGCAGGATCTCCCCTTGCAACATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000187 GO:0001932 GO:0001934 GO:0002376 GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009628 GO:0009631 GO:0009651 GO:0009814 GO:0009893 GO:0009914 GO:0009966 GO:0009967 GO:0009987 GO:0010051 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0098542 GO:0140096 GO:1901564 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

355

Amino Acids

39.8

Weight (kDa)

6.15

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 73 - 330 3.8e-71 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 73 - 326 1e-40 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 172 - 267 9.2e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 511
Acc36I ACCTGC 1 cut(s) 511
Acc65I GGTACC 1 cut(s) 416
AccB1I GGYRCC 1 cut(s) 416
AciI CCGC 3 cut(s) 87, 164, 328
AclWI GGATC 3 cut(s) 143, 935, 1051
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 491
AdeI CACNNNGTG 1 cut(s) 878
AfaI GTAC 7 cut(s) 342, 418, 440, 768, 804, 933, 998
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 7 cut(s) 158, 176, 295, 317, 352, 426, 842
AjiI CACGTC 1 cut(s) 660
AjnI CCWGG 2 cut(s) 111, 756
AluBI AGCT 3 cut(s) 34, 735, 962
AluI AGCT 3 cut(s) 34, 735, 962
Alw26I GTCTC 1 cut(s) 970
AlwI GGATC 3 cut(s) 143, 935, 1051
AlwNI CAGNNNCTG 1 cut(s) 526
ApeKI GCWGC 2 cut(s) 506, 526
ApoI RAATTY 1 cut(s) 158
AseI ATTAAT 1 cut(s) 357
Asp718I GGTACC 1 cut(s) 416
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 1 cut(s) 1024
AvaII GGWCC 1 cut(s) 20
AxyI CCTNAGG 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 883
BanI GGYRCC 1 cut(s) 416
BbsI GAAGAC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 493, 513
BccI CCATC 5 cut(s) 95, 440, 576, 863, 964
BciT130I CCWGG 2 cut(s) 113, 758
BcoDI GTCTC 1 cut(s) 970
BfaI CTAG 1 cut(s) 434
BfmI CTRYAG 1 cut(s) 527
BfuAI ACCTGC 1 cut(s) 511
BglII AGATCT 1 cut(s) 967
BisI GCNGC 3 cut(s) 88, 507, 527
BlsI GCNGC 3 cut(s) 89, 508, 528
BmcAI AGTACT 1 cut(s) 768
Bme1390I CCNGG 2 cut(s) 113, 758
Bme18I GGWCC 1 cut(s) 20
BmgBI CACGTC 1 cut(s) 660
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 4 cut(s) 13, 22, 418, 755
BmrFI CCNGG 2 cut(s) 113, 758
BmsI GCATC 1 cut(s) 188
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BpmI CTGGAG 2 cut(s) 794, 885
BpuEI CTTGAG 2 cut(s) 14, 948
BsaBI GATNNNNATC 1 cut(s) 105
BsaJI CCNNGG 1 cut(s) 757
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bse1I ACTGG 3 cut(s) 244, 280, 397
Bse21I CCTNAGG 1 cut(s) 16
Bse8I GATNNNNATC 1 cut(s) 105
BseBI CCWGG 2 cut(s) 113, 758
BseDI CCNNGG 1 cut(s) 757
BseGI GGATG 5 cut(s) 106, 429, 451, 564, 979
BseJI GATNNNNATC 1 cut(s) 105
BseMII CTCAG 1 cut(s) 965
BseNI ACTGG 3 cut(s) 244, 280, 397
BseRI GAGGAG 2 cut(s) 36, 624
BseSI GKGCMC 1 cut(s) 883
BseXI GCAGC 2 cut(s) 493, 513
BshNI GGYRCC 1 cut(s) 416
BslFI GGGAC 1 cut(s) 587
BsmAI GTCTC 1 cut(s) 970
BsmFI GGGAC 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 883
Bsp1407I TGTACA 2 cut(s) 340, 931
Bsp143I GATC 7 cut(s) 106, 135, 811, 889, 940, 967, 1043
BspACI CCGC 3 cut(s) 87, 164, 328
BspCNI CTCAG 1 cut(s) 966
BspLI GGNNCC 4 cut(s) 13, 22, 418, 755
BspMAI CTGCAG 1 cut(s) 531
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 3 cut(s) 143, 935, 1051
BspT107I GGYRCC 1 cut(s) 416
BsrGI TGTACA 2 cut(s) 340, 931
BsrI ACTGG 3 cut(s) 244, 280, 397
BssECI CCNNGG 1 cut(s) 757
BssMI GATC 7 cut(s) 106, 135, 811, 889, 940, 967, 1043
Bst2UI CCWGG 2 cut(s) 113, 758
Bst6I CTCTTC 1 cut(s) 312
BstAPI GCANNNNNTGC 2 cut(s) 526, 878
BstAUI TGTACA 2 cut(s) 340, 931
BstC8I GCNNGC 2 cut(s) 332, 655
BstDEI CTNAG 3 cut(s) 16, 69, 974
BstF5I GGATG 5 cut(s) 106, 429, 451, 564, 979
BstKTI GATC 7 cut(s) 109, 138, 814, 892, 943, 970, 1046
BstMAI GTCTC 1 cut(s) 970
BstMBI GATC 7 cut(s) 106, 135, 811, 889, 940, 967, 1043
BstMWI GCNNNNNNNGC 4 cut(s) 526, 650, 732, 878
BstNI CCWGG 2 cut(s) 113, 758
BstNSI RCATGY 2 cut(s) 930, 997
BstSCI CCNGG 2 cut(s) 111, 756
BstSFI CTRYAG 1 cut(s) 527
BstSLI GKGCMC 1 cut(s) 883
BstV1I GCAGC 2 cut(s) 493, 513
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 3 cut(s) 940, 967, 1043
BstYI RGATCY 3 cut(s) 940, 967, 1043
Bsu36I CCTNAGG 1 cut(s) 16
BtrI CACGTC 1 cut(s) 660
BtsCI GGATG 5 cut(s) 106, 429, 451, 564, 979
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 2 cut(s) 332, 655
CaiI CAGNNNCTG 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 20
Csp6I GTAC 7 cut(s) 341, 417, 439, 767, 803, 932, 997
CviAII CATG 4 cut(s) 442, 825, 927, 994
CviJI RGCY 5 cut(s) 34, 203, 735, 756, 962
CviKI_1 RGCY 5 cut(s) 34, 203, 735, 756, 962
CviQI GTAC 7 cut(s) 341, 417, 439, 767, 803, 932, 997
DdeI CTNAG 3 cut(s) 16, 69, 974
DpnI GATC 7 cut(s) 108, 137, 813, 891, 942, 969, 1045
DpnII GATC 7 cut(s) 106, 135, 811, 889, 940, 967, 1043
DraI TTTAAA 1 cut(s) 580
DraIII CACNNNGTG 1 cut(s) 878
Eam1104I CTCTTC 1 cut(s) 312
EarI CTCTTC 1 cut(s) 312
EciI GGCGGA 1 cut(s) 153
Eco47I GGWCC 1 cut(s) 20
Eco57I CTGAAG 1 cut(s) 491
Eco81I CCTNAGG 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 20
EcoRII CCWGG 2 cut(s) 111, 756
FaeI CATG 4 cut(s) 445, 828, 930, 997
FaqI GGGAC 1 cut(s) 587
FatI CATG 4 cut(s) 441, 824, 926, 993
FauNDI CATATG 1 cut(s) 799
Fnu4HI GCNGC 3 cut(s) 88, 507, 527
FokI GGATG 5 cut(s) 113, 416, 458, 551, 966
Fsp4HI GCNGC 3 cut(s) 88, 507, 527
FspBI CTAG 1 cut(s) 434
GluI GCNGC 3 cut(s) 88, 507, 527
GsuI CTGGAG 2 cut(s) 794, 885
Hin1II CATG 4 cut(s) 445, 828, 930, 997
HinfI GANTC 6 cut(s) 79, 212, 274, 320, 487, 714
HphI GGTGA 1 cut(s) 1024
Hpy166II GTNNAC 1 cut(s) 932
Hpy188I TCNGA 7 cut(s) 135, 184, 306, 471, 664, 889, 945
Hpy188III TCNNGA 7 cut(s) 50, 76, 491, 572, 902, 965, 973
Hpy8I GTNNAC 1 cut(s) 932
HpyAV CCTTC 6 cut(s) 289, 412, 469, 594, 894, 940
HpyCH4IV ACGT 2 cut(s) 92, 659
HpyCH4V TGCA 8 cut(s) 253, 290, 529, 644, 782, 926, 1009, 1055
HpyF10VI GCNNNNNNNGC 4 cut(s) 526, 650, 732, 878
HpyF3I CTNAG 3 cut(s) 16, 69, 974
HpySE526I ACGT 2 cut(s) 92, 659
Hsp92II CATG 4 cut(s) 445, 828, 930, 997
KpnI GGTACC 1 cut(s) 420
Kzo9I GATC 7 cut(s) 106, 135, 811, 889, 940, 967, 1043
LmnI GCTCC 1 cut(s) 753
Lsp1109I GCAGC 2 cut(s) 493, 513
LweI GCATC 1 cut(s) 188
MaeI CTAG 1 cut(s) 434
MaeII ACGT 2 cut(s) 92, 659
MaeIII GTNAC 3 cut(s) 392, 743, 830
MalI GATC 7 cut(s) 108, 137, 813, 891, 942, 969, 1045
MboI GATC 7 cut(s) 106, 135, 811, 889, 940, 967, 1043
MboII GAAGA 5 cut(s) 16, 329, 458, 484, 536
MfeI CAATTG 1 cut(s) 254
MflI RGATCY 3 cut(s) 940, 967, 1043
MhlI GDGCHC 1 cut(s) 883
MluCI AATT 9 cut(s) 158, 254, 300, 347, 354, 589, 727, 791, 893
MlyI GAGTC 4 cut(s) 73, 206, 268, 329
MnlI CCTC 6 cut(s) 38, 54, 57, 218, 602, 1033
MseI TTAA 8 cut(s) 96, 117, 219, 357, 579, 596, 618, 1063
MslI CAYNNNNRTG 2 cut(s) 370, 992
MspR9I CCNGG 2 cut(s) 113, 758
MunI CAATTG 1 cut(s) 254
MvaI CCWGG 2 cut(s) 113, 758
MwoI GCNNNNNNNGC 4 cut(s) 526, 650, 732, 878
NdeI CATATG 1 cut(s) 799
NdeII GATC 7 cut(s) 106, 135, 811, 889, 940, 967, 1043
NlaIII CATG 4 cut(s) 445, 828, 930, 997
NlaIV GGNNCC 4 cut(s) 13, 22, 418, 755
NmuCI GTSAC 1 cut(s) 743
NspI RCATGY 2 cut(s) 930, 997
PaqCI CACCTGC 1 cut(s) 511
PciI ACATGT 1 cut(s) 993
PfeI GAWTC 2 cut(s) 487, 714
PkrI GCNGC 3 cut(s) 89, 508, 528
PleI GAGTC 4 cut(s) 73, 206, 268, 328
PpsI GAGTC 4 cut(s) 73, 206, 268, 328
PpuMI RGGWCCY 1 cut(s) 20
PscI ACATGT 1 cut(s) 993
PshBI ATTAAT 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 20
Psp6I CCWGG 2 cut(s) 111, 756
PspGI CCWGG 2 cut(s) 111, 756
PspN4I GGNNCC 4 cut(s) 13, 22, 418, 755
PspPI GGNCC 1 cut(s) 20
PspPPI RGGWCCY 1 cut(s) 20
PstI CTGCAG 1 cut(s) 531
PstNI CAGNNNCTG 1 cut(s) 526
PsuI RGATCY 3 cut(s) 940, 967, 1043
RsaI GTAC 7 cut(s) 342, 418, 440, 768, 804, 933, 998
RsaNI GTAC 7 cut(s) 341, 417, 439, 767, 803, 932, 997
RseI CAYNNNNRTG 2 cut(s) 370, 992
SaqAI TTAA 8 cut(s) 96, 117, 219, 357, 579, 596, 618, 1063
SatI GCNGC 3 cut(s) 88, 507, 527
Sau3AI GATC 7 cut(s) 106, 135, 811, 889, 940, 967, 1043
Sau96I GGNCC 1 cut(s) 20
ScaI AGTACT 1 cut(s) 768
SchI GAGTC 4 cut(s) 73, 206, 268, 329
ScrFI CCNGG 2 cut(s) 113, 758
SduI GDGCHC 1 cut(s) 883
SfaNI GCATC 1 cut(s) 188
SfcI CTRYAG 1 cut(s) 527
SinI GGWCC 1 cut(s) 20
SmiMI CAYNNNNRTG 2 cut(s) 370, 992
SmlI CTYRAG 2 cut(s) 29, 963
SmoI CTYRAG 2 cut(s) 29, 963
Sse9I AATT 9 cut(s) 158, 254, 300, 347, 354, 589, 727, 791, 893
SsiI CCGC 3 cut(s) 87, 164, 328
SspMI CTAG 1 cut(s) 434
StyD4I CCNGG 2 cut(s) 111, 756
TaiI ACGT 2 cut(s) 95, 662
TaqI TCGA 2 cut(s) 210, 215
TasI AATT 9 cut(s) 158, 254, 300, 347, 354, 589, 727, 791, 893
TatI WGTACW 4 cut(s) 340, 438, 766, 931
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 2 cut(s) 487, 714
Tru1I TTAA 8 cut(s) 96, 117, 219, 357, 579, 596, 618, 1063
Tru9I TTAA 8 cut(s) 96, 117, 219, 357, 579, 596, 618, 1063
TseFI GTSAC 1 cut(s) 743
TseI GCWGC 2 cut(s) 506, 526
Tsp45I GTSAC 1 cut(s) 743
TspDTI ATGAA 8 cut(s) 17, 52, 69, 323, 536, 670, 879, 976
TspGWI ACGGA 1 cut(s) 641
VpaK11BI GGWCC 1 cut(s) 20
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 1 cut(s) 158
XceI RCATGY 2 cut(s) 930, 997
XspI CTAG 1 cut(s) 434
ZrmI AGTACT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.