RLG00000016450

mitogen-activated protein kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006526
Physical Location & Seq
Reverse (-)
6732347 .. 6735218
2872 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016450

Sequence Viewer

Length: 1065 bp
ATGAACAAGGGAATCTTAGGCTCTAAGCTCAAGCTTACTCTTCCTCCTCCTGATGAAGATTCTATCAAGAAGTTCCTGACTCAAAGTGGTACCTTTATGGATGGGGATCTGCTGGTGAATAGGGAAGGGGTTCGGATCGTCTCTCATAGAGAAGTTGAAGTTCCAGCCCCTATTCAAGCAGCAGACAATCAGATGATTTTAGCAGATCTCTACGTGGTTAAAGTTATTGGTAAAGGAAATGGTGGGGTTGTGCAATTGGTTCAGCACAAATGGACTGGCCAGTTTTTTGCATTGAAGGTAATCCAAATGAATATTCAGGAGTCTATTCGCAAGCAGATTGCACAAGAATTGAAAATTAATCAATCATCACAATGTCCCTATATTGTTGTATGTTACCGGTCCTTCTATGATAATGGTTGCATTTCAATTATCCTAGAGTACATGGATGGTGGATCTTTAGCAGATCTTCTGAGAAAAGTCAGATCAATTCCAGAGCCATATATTTCTGCTATGTGTAAGCAGGTGCTGCGTGGTTTGTTGTATCTTCATCATGAAAAACACATAATTCACAGGGACTTCAAACCCTCAAACTTGTTAATAAATCATAGAGGAGAAATTAAGATAACTGACTTTGGTGTCAGTGCAATAAAGGCAAACACCTCTGAACAGGCAAATACTTTTGTTGGCACATACAACTATATGTCGCCAGAGAGAATACAAGGTAACAGCTATAGCTACAAAAGTGACATTTGGAGCTTGGGCTTAGTACTACTGGAGTGTGCAACAGGTAAATTCCCATATACACCACCAGATCAAAGTGAAGGATGGGAAAACTTTTTTGAGCTTATGAGTGCTGTTGTTGACTTGCCACCACCTTCTGCACCTTCCGATCAGTTTTCTCCAGAGTTCTGCTCATTTATTTCTGCATGCGTACAGAAAGATCCTAAAAAGAGATTGTCAGCACATGATCTCCTAGTGCATCCTTTTATCAGCATGTACGACGACTTGAATATCGACCTTTCGTCTTACTTCACTGAAGCAGGATCTCCACTCGCAACCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000187 GO:0001932 GO:0001934 GO:0002376 GO:0003002 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004708 GO:0004712 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007346 GO:0007389 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009628 GO:0009631 GO:0009651 GO:0009814 GO:0009893 GO:0009914 GO:0009966 GO:0009967 GO:0009987 GO:0010051 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0010817 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032501 GO:0032502 GO:0033554 GO:0033674 GO:0035556 GO:0036211 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043207 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0045859 GO:0045860 GO:0045937 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051234 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051704 GO:0051707 GO:0051716 GO:0051726 GO:0060255 GO:0060918 GO:0065007 GO:0065008 GO:0065009 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0098542 GO:0140096 GO:1901564 GO:1902531 GO:1902533
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

355

Amino Acids

39.71

Weight (kDa)

6.15

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 72 - 330 5.5e-72 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 73 - 324 4.5e-38 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 174 - 281 2.3e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 511
AasI GACNNNNNNGTC 1 cut(s) 635
Acc36I ACCTGC 1 cut(s) 511
Acc65I GGTACC 1 cut(s) 89
AccB1I GGYRCC 1 cut(s) 89
AclWI GGATC 5 cut(s) 114, 143, 460, 935, 1051
AcoI YGGCCR 1 cut(s) 277
AcsI RAATTY 1 cut(s) 791
AcuI CTGAAG 1 cut(s) 1056
AfaI GTAC 5 cut(s) 91, 440, 768, 933, 998
AgeI ACCGGT 1 cut(s) 396
AgsI TTSAA 7 cut(s) 158, 176, 295, 352, 426, 580, 1009
AhdI GACNNNNNGTC 1 cut(s) 1021
AjuI GAANNNNNNNTTGG 2 cut(s) 297, 329
AluBI AGCT 6 cut(s) 28, 34, 729, 735, 756, 844
AluI AGCT 6 cut(s) 28, 34, 729, 735, 756, 844
Alw26I GTCTC 1 cut(s) 145
AlwI GGATC 5 cut(s) 114, 143, 460, 935, 1051
AlwNI CAGNNNCTG 1 cut(s) 526
AoxI GGCC 1 cut(s) 277
ApeKI GCWGC 2 cut(s) 179, 526
ApoI RAATTY 1 cut(s) 791
AseI ATTAAT 1 cut(s) 357
AsiGI ACCGGT 1 cut(s) 396
Asp700I GAANNNNTTC 1 cut(s) 129
Asp718I GGTACC 1 cut(s) 89
AspS9I GGNCC 1 cut(s) 399
AsuHPI GGTGA 1 cut(s) 127
AvaII GGWCC 1 cut(s) 399
BalI TGGCCA 1 cut(s) 279
BanI GGYRCC 1 cut(s) 89
BbvI GCAGC 2 cut(s) 191, 513
BccI CCATC 3 cut(s) 95, 440, 819
BcoDI GTCTC 1 cut(s) 145
BfaI CTAG 2 cut(s) 434, 974
BfmI CTRYAG 1 cut(s) 730
BfuAI ACCTGC 1 cut(s) 511
BglII AGATCT 2 cut(s) 205, 463
BisI GCNGC 2 cut(s) 180, 527
BlsI GCNGC 2 cut(s) 181, 528
BmcAI AGTACT 1 cut(s) 768
Bme18I GGWCC 1 cut(s) 399
BmeRI GACNNNNNGTC 1 cut(s) 1021
BmgT120I GGNCC 1 cut(s) 399
BmiI GGNNCC 1 cut(s) 91
BmsI GCATC 1 cut(s) 988
BplI GAGNNNNNCTC 2 cut(s) 896, 928
BpmI CTGGAG 2 cut(s) 794, 885
BpuEI CTTGAG 1 cut(s) 14
BsaAI YACGTR 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 105
BsaWI WCCGGW 1 cut(s) 396
BsaXI ACNNNNNCTCC 4 cut(s) 28, 58, 954, 984
Bse118I RCCGGY 1 cut(s) 396
Bse1I ACTGG 3 cut(s) 280, 280, 777
Bse8I GATNNNNATC 1 cut(s) 105
BseGI GGATG 4 cut(s) 106, 451, 830, 979
BseJI GATNNNNATC 1 cut(s) 105
BseMII CTCAG 1 cut(s) 461
BseNI ACTGG 3 cut(s) 280, 280, 777
BseRI GAGGAG 2 cut(s) 36, 624
BseXI GCAGC 2 cut(s) 191, 513
BsgI GTGCAG 1 cut(s) 864
BshFI GGCC 1 cut(s) 279
BshNI GGYRCC 1 cut(s) 89
BshTI ACCGGT 1 cut(s) 396
BsiSI CCGG 1 cut(s) 397
BslFI GGGAC 2 cut(s) 360, 587
BsmAI GTCTC 1 cut(s) 145
BsmBI CGTCTC 1 cut(s) 145
BsmFI GGGAC 2 cut(s) 360, 587
BsnI GGCC 1 cut(s) 279
BspANI GGCC 1 cut(s) 279
BspCNI CTCAG 1 cut(s) 462
BspHI TCATGA 1 cut(s) 550
BspLI GGNNCC 1 cut(s) 91
BspMI ACCTGC 1 cut(s) 511
BspPI GGATC 5 cut(s) 114, 143, 460, 935, 1051
BspT107I GGYRCC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 396
BsrI ACTGG 3 cut(s) 280, 280, 777
BssAI RCCGGY 1 cut(s) 396
Bst6I CTCTTC 1 cut(s) 45
BstAPI GCANNNNNTGC 1 cut(s) 526
BstBAI YACGTR 1 cut(s) 214
BstC8I GCNNGC 2 cut(s) 332, 928
BstDEI CTNAG 4 cut(s) 16, 24, 470, 763
BstF5I GGATG 4 cut(s) 106, 451, 830, 979
BstMAI GTCTC 1 cut(s) 145
BstMWI GCNNNNNNNGC 2 cut(s) 526, 650
BstNSI RCATGY 2 cut(s) 930, 997
BstSFI CTRYAG 1 cut(s) 730
BstV1I GCAGC 2 cut(s) 191, 513
BstX2I RGATCY 6 cut(s) 106, 205, 452, 463, 940, 1043
BstYI RGATCY 6 cut(s) 106, 205, 452, 463, 940, 1043
BsuRI GGCC 1 cut(s) 279
BtsCI GGATG 4 cut(s) 106, 451, 830, 979
BtsIMutI CAGTG 2 cut(s) 646, 1032
BveI ACCTGC 1 cut(s) 511
Cac8I GCNNGC 2 cut(s) 332, 928
CaiI CAGNNNCTG 1 cut(s) 526
CciI TCATGA 1 cut(s) 550
Cfr10I RCCGGY 1 cut(s) 396
Cfr13I GGNCC 1 cut(s) 399
Csp6I GTAC 5 cut(s) 90, 439, 767, 932, 997
CspAI ACCGGT 1 cut(s) 396
CviAII CATG 5 cut(s) 442, 551, 927, 965, 994
CviQI GTAC 5 cut(s) 90, 439, 767, 932, 997
DdeI CTNAG 4 cut(s) 16, 24, 470, 763
DrdI GACNNNNNNGTC 1 cut(s) 635
DriI GACNNNNNGTC 1 cut(s) 1021
DseDI GACNNNNNNGTC 1 cut(s) 635
EaeI YGGCCR 1 cut(s) 277
Eam1104I CTCTTC 1 cut(s) 45
Eam1105I GACNNNNNGTC 1 cut(s) 1021
EarI CTCTTC 1 cut(s) 45
Eco47I GGWCC 1 cut(s) 399
Eco57I CTGAAG 1 cut(s) 1056
Esp3I CGTCTC 1 cut(s) 145
FaeI CATG 5 cut(s) 445, 554, 930, 968, 997
FalI AAGNNNNNCTT 1 cut(s) 31
FaqI GGGAC 2 cut(s) 360, 587
FatI CATG 5 cut(s) 441, 550, 926, 964, 993
Fnu4HI GCNGC 2 cut(s) 180, 527
FokI GGATG 4 cut(s) 113, 458, 837, 966
Fsp4HI GCNGC 2 cut(s) 180, 527
FspBI CTAG 2 cut(s) 434, 974
GluI GCNGC 2 cut(s) 180, 527
GsuI CTGGAG 2 cut(s) 794, 885
HaeIII GGCC 1 cut(s) 279
HapII CCGG 1 cut(s) 397
Hin1II CATG 5 cut(s) 445, 554, 930, 968, 997
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 4 cut(s) 12, 59, 79, 320
HpaII CCGG 1 cut(s) 397
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 1 cut(s) 862
Hpy188I TCNGA 6 cut(s) 135, 192, 471, 482, 664, 889
Hpy188III TCNNGA 7 cut(s) 50, 67, 76, 317, 491, 551, 902
Hpy8I GTNNAC 1 cut(s) 862
Hpy99I CGWCG 1 cut(s) 1004
HpyAV CCTTC 6 cut(s) 119, 289, 412, 815, 885, 894
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 9 cut(s) 253, 290, 341, 420, 644, 782, 881, 926, 979
HpyF10VI GCNNNNNNNGC 2 cut(s) 526, 650
HpyF3I CTNAG 4 cut(s) 16, 24, 470, 763
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 5 cut(s) 445, 554, 930, 968, 997
KpnI GGTACC 1 cut(s) 93
LmnI GCTCC 1 cut(s) 753
Lsp1109I GCAGC 2 cut(s) 191, 513
LweI GCATC 1 cut(s) 988
MaeI CTAG 2 cut(s) 434, 974
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 3 cut(s) 392, 722, 743
MboII GAAGA 4 cut(s) 32, 68, 458, 536
MfeI CAATTG 1 cut(s) 254
MflI RGATCY 6 cut(s) 106, 205, 452, 463, 940, 1043
MlsI TGGCCA 1 cut(s) 279
MluCI AATT 8 cut(s) 254, 347, 354, 426, 486, 564, 615, 791
MluNI TGGCCA 1 cut(s) 279
MlyI GAGTC 2 cut(s) 73, 329
MnlI CCTC 5 cut(s) 54, 57, 595, 602, 670
Mox20I TGGCCA 1 cut(s) 279
MroXI GAANNNNTTC 1 cut(s) 129
MscI TGGCCA 1 cut(s) 279
MseI TTAA 5 cut(s) 219, 357, 596, 618, 1063
MslI CAYNNNNRTG 1 cut(s) 370
Msp20I TGGCCA 1 cut(s) 279
MspI CCGG 1 cut(s) 397
MunI CAATTG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 526, 650
NlaIII CATG 5 cut(s) 445, 554, 930, 968, 997
NlaIV GGNNCC 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 743
NspI RCATGY 2 cut(s) 930, 997
PaeI GCATGC 1 cut(s) 930
PagI TCATGA 1 cut(s) 550
PaqCI CACCTGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 129
PfeI GAWTC 2 cut(s) 12, 59
PinAI ACCGGT 1 cut(s) 396
PkrI GCNGC 2 cut(s) 181, 528
PleI GAGTC 2 cut(s) 73, 328
PpsI GAGTC 2 cut(s) 73, 328
Ppu21I YACGTR 1 cut(s) 214
PshBI ATTAAT 1 cut(s) 357
PspN4I GGNNCC 1 cut(s) 91
PspPI GGNCC 1 cut(s) 399
PstNI CAGNNNCTG 1 cut(s) 526
PsuI RGATCY 6 cut(s) 106, 205, 452, 463, 940, 1043
RsaI GTAC 5 cut(s) 91, 440, 768, 933, 998
RsaNI GTAC 5 cut(s) 90, 439, 767, 932, 997
RseI CAYNNNNRTG 1 cut(s) 370
SaqAI TTAA 5 cut(s) 219, 357, 596, 618, 1063
SatI GCNGC 2 cut(s) 180, 527
Sau96I GGNCC 1 cut(s) 399
ScaI AGTACT 1 cut(s) 768
SchI GAGTC 2 cut(s) 73, 329
SfaNI GCATC 1 cut(s) 988
SfcI CTRYAG 1 cut(s) 730
SinI GGWCC 1 cut(s) 399
SmiMI CAYNNNNRTG 1 cut(s) 370
SmlI CTYRAG 1 cut(s) 29
SmoI CTYRAG 1 cut(s) 29
SphI GCATGC 1 cut(s) 930
Sse9I AATT 8 cut(s) 254, 347, 354, 426, 486, 564, 615, 791
SspI AATATT 1 cut(s) 313
SspMI CTAG 2 cut(s) 434, 974
TaiI ACGT 1 cut(s) 216
TaqI TCGA 1 cut(s) 1014
TasI AATT 8 cut(s) 254, 347, 354, 426, 486, 564, 615, 791
TatI WGTACW 2 cut(s) 438, 766
TfiI GAWTC 2 cut(s) 12, 59
Tru1I TTAA 5 cut(s) 219, 357, 596, 618, 1063
Tru9I TTAA 5 cut(s) 219, 357, 596, 618, 1063
TscAI CASTG 2 cut(s) 646, 1039
TseFI GTSAC 1 cut(s) 743
TseI GCWGC 2 cut(s) 179, 526
Tsp45I GTSAC 1 cut(s) 743
TspDTI ATGAA 5 cut(s) 17, 69, 323, 536, 567
TspRI CASTG 2 cut(s) 646, 1039
VpaK11BI GGWCC 1 cut(s) 399
VspI ATTAAT 1 cut(s) 357
XapI RAATTY 1 cut(s) 791
XceI RCATGY 2 cut(s) 930, 997
XmnI GAANNNNTTC 1 cut(s) 129
XspI CTAG 2 cut(s) 434, 974
ZrmI AGTACT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.