FvH4_1g10500

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014634
Physical Location & Seq
Forward (+)
5734599 .. 5735162
564 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10500.t1

Sequence Viewer

Length: 564 bp
ATGCCTCTCTCTGTGGTTCATGCTCTACTTGTAGCCCTCCTCTTAGTTAAAGCCACCTCCACCTCTCACCACCACCACCACCACCATCATCATCACCACAACCAACTCAAGTCCCTCCACTTCGCGCTCTTTCAACACGAAACCATCAACAAAACCGGATACATTGTTGTTAATGGCGTGGCCGGAACAGGTGTTAGTCAAACCACAACTCCTTTCGGCACCCTCTTCGTTTTCCAGGATCCGATGACTGTCACAGCCAATCGCTCTTCAAAGGTAGCTGGGAGAGCTCAAGGAACTTCCATCACTTCCAGCTTGGATGGGCTTGAGAGCATTTCCATTGCTAAGATAACATTGCGGTTGAAGCACCACCGTGGTTCGATTTCCATTGTCGGAGGCACACATAACATCAAGCCGGCTGATCATCCTGTTGTGGGAGGCACCGGTGATTTCAAGCTTGTGCAGGGCTATGTGACATCGTCTCCAGTCAATCTTACGGGCTTAACTGTTACCTACAAGATTGCATTCCATCTTTACTGGCCTCCCTATGCAACTCAAGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

188

Amino Acids

20.17

Weight (kDa)

9.87

Isoelectric Point (pI)

20.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 40 - 172 9.1e-29 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10500
malus_domestica MD02G1118100.v1.1 MD02G1118400.v1.1
prunus_persica Prupe.7G181300_v2.0.a1
pyrus_communis pycom02g09170 pycom02g09180 pycom15g20710
rosa_chinensis RchiOBHm_Chr2g0097361
rosa_laevigata RLG00000016720
rosa_multiflora Rmu_sc0001060.1_g000010
rosa_roxburghii Rroxscaffold_2G00144740
rosa_rugosa Rorug02G0066000
rosa_samantha Rh2AG112800 Rh2BG115600 Rh2CG117800 Rh2DG116800
rosa_wichuraiana Rw2G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 218, 437
AccII CGCG 1 cut(s) 125
AciI CCGC 1 cut(s) 355
AclWI GGATC 2 cut(s) 233, 246
AcoI YGGCCR 1 cut(s) 180
AfiI CCNNNNNNNGG 1 cut(s) 431
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 4 cut(s) 134, 270, 361, 451
AjnI CCWGG 1 cut(s) 234
AleI CACNNNNGTG 1 cut(s) 369
AluBI AGCT 5 cut(s) 278, 287, 312, 454, 557
AluI AGCT 5 cut(s) 278, 287, 312, 454, 557
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 483
AlwI GGATC 2 cut(s) 233, 246
AoxI GGCC 2 cut(s) 180, 536
AsiGI ACCGGT 1 cut(s) 440
AspLEI GCGC 1 cut(s) 127
AsuHPI GGTGA 3 cut(s) 59, 86, 455
BamHI GGATCC 1 cut(s) 238
BanI GGYRCC 2 cut(s) 218, 437
BanII GRGCYC 1 cut(s) 289
Bbv12I GWGCWC 1 cut(s) 289
BccI CCATC 5 cut(s) 93, 152, 308, 311, 534
BcgI CGANNNNNNTGC 2 cut(s) 208, 242
BciT130I CCWGG 1 cut(s) 236
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 483
BfuI GTATCC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 236
BmiI GGNNCC 3 cut(s) 220, 240, 439
BmrFI CCNGG 1 cut(s) 236
BpmI CTGGAG 1 cut(s) 465
BpuEI CTTGAG 4 cut(s) 92, 273, 344, 537
BsaJI CCNNGG 1 cut(s) 370
BsaWI WCCGGW 2 cut(s) 155, 440
BsaXI ACNNNNNCTCC 4 cut(s) 193, 223, 463, 493
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse118I RCCGGY 2 cut(s) 412, 440
Bse1I ACTGG 2 cut(s) 482, 539
Bse3DI GCAATG 2 cut(s) 336, 350
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 2 cut(s) 322, 421
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMI GCAATG 2 cut(s) 336, 350
BseNI ACTGG 2 cut(s) 482, 539
BseRI GAGGAG 1 cut(s) 29
BseYI CCCAGC 1 cut(s) 278
BsgI GTGCAG 1 cut(s) 479
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 2 cut(s) 182, 538
BshNI GGYRCC 2 cut(s) 218, 437
BshTI ACCGGT 1 cut(s) 440
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 4 cut(s) 156, 183, 413, 441
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 1 cut(s) 483
BsmBI CGTCTC 1 cut(s) 483
BsmFI GGGAC 1 cut(s) 97
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 2 cut(s) 182, 538
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 2 cut(s) 238, 418
BspACI CCGC 1 cut(s) 355
BspANI GGCC 2 cut(s) 182, 538
BspFNI CGCG 1 cut(s) 125
BspLI GGNNCC 3 cut(s) 220, 240, 439
BspPI GGATC 2 cut(s) 233, 246
BspQI GCTCTTC 1 cut(s) 271
BspT107I GGYRCC 2 cut(s) 218, 437
BsrDI GCAATG 2 cut(s) 336, 350
BsrFI RCCGGY 2 cut(s) 412, 440
BsrI ACTGG 2 cut(s) 482, 539
BssAI RCCGGY 2 cut(s) 412, 440
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 2 cut(s) 238, 418
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 3 cut(s) 250, 371, 505
Bst6I CTCTTC 2 cut(s) 230, 271
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 2 cut(s) 43, 342
BstDSI CCRYGG 1 cut(s) 370
BstF5I GGATG 2 cut(s) 322, 421
BstFNI CGCG 1 cut(s) 125
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 2 cut(s) 241, 421
BstMAI GTCTC 1 cut(s) 483
BstMBI GATC 2 cut(s) 238, 418
BstMWI GCNNNNNNNGC 3 cut(s) 284, 361, 554
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 234
BstUI CGCG 1 cut(s) 125
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 2 cut(s) 182, 538
BtgI CCRYGG 1 cut(s) 370
BtsCI GGATG 2 cut(s) 322, 421
Cac8I GCNNGC 1 cut(s) 414
CfoI GCGC 1 cut(s) 127
Cfr10I RCCGGY 2 cut(s) 412, 440
CspAI ACCGGT 1 cut(s) 440
CviAII CATG 1 cut(s) 20
DdeI CTNAG 2 cut(s) 43, 342
DpnI GATC 2 cut(s) 240, 420
DpnII GATC 2 cut(s) 238, 418
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 2 cut(s) 230, 271
EarI CTCTTC 2 cut(s) 230, 271
Ecl136II GAGCTC 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 289
Eco53kI GAGCTC 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 287
EcoRII CCWGG 1 cut(s) 234
EcoT38I GRGCYC 1 cut(s) 289
Esp3I CGTCTC 1 cut(s) 483
FaeI CATG 1 cut(s) 23
FaiI YATR 4 cut(s) 21, 402, 468, 546
FaqI GGGAC 1 cut(s) 97
FatI CATG 1 cut(s) 19
FbaI TGATCA 1 cut(s) 418
FokI GGATG 2 cut(s) 329, 408
FriOI GRGCYC 1 cut(s) 289
GlaI GCGC 1 cut(s) 126
GsaI CCCAGC 1 cut(s) 282
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 2 cut(s) 182, 538
HapII CCGG 4 cut(s) 156, 183, 413, 441
HhaI GCGC 1 cut(s) 127
Hin1II CATG 1 cut(s) 23
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HindIII AAGCTT 2 cut(s) 452, 555
HpaII CCGG 4 cut(s) 156, 183, 413, 441
HphI GGTGA 3 cut(s) 59, 86, 455
Hpy188I TCNGA 2 cut(s) 243, 392
HpyCH4III ACNGT 3 cut(s) 250, 371, 505
HpyCH4V TGCA 3 cut(s) 460, 521, 548
HpyF10VI GCNNNNNNNGC 3 cut(s) 284, 361, 554
HpyF3I CTNAG 2 cut(s) 43, 342
Hsp92II CATG 1 cut(s) 23
HspAI GCGC 1 cut(s) 125
KroI GCCGGC 1 cut(s) 412
KroNI GCCGGC 1 cut(s) 414
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 2 cut(s) 238, 418
LguI GCTCTTC 1 cut(s) 271
MaeIII GTNAC 3 cut(s) 250, 469, 505
MalI GATC 2 cut(s) 240, 420
MboI GATC 2 cut(s) 238, 418
MboII GAAGA 2 cut(s) 217, 258
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 1 cut(s) 289
MmeI TCCRAC 1 cut(s) 370
MroNI GCCGGC 1 cut(s) 412
MseI TTAA 4 cut(s) 48, 171, 500, 562
MslI CAYNNNNRTG 1 cut(s) 369
MspI CCGG 4 cut(s) 156, 183, 413, 441
MspR9I CCNGG 1 cut(s) 236
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 3 cut(s) 284, 361, 554
NaeI GCCGGC 1 cut(s) 414
NdeII GATC 2 cut(s) 238, 418
NgoMIV GCCGGC 1 cut(s) 412
NlaIII CATG 1 cut(s) 23
NlaIV GGNNCC 3 cut(s) 220, 240, 439
NmuCI GTSAC 2 cut(s) 250, 469
OliI CACNNNNGTG 1 cut(s) 369
PciSI GCTCTTC 1 cut(s) 271
PctI GAATGC 1 cut(s) 521
PdiI GCCGGC 1 cut(s) 414
PflFI GACNNNGTC 1 cut(s) 475
PfoI TCCNGGA 1 cut(s) 234
PinAI ACCGGT 1 cut(s) 440
Psp124BI GAGCTC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 234
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 3 cut(s) 220, 240, 439
PsuI RGATCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 475
RseI CAYNNNNRTG 1 cut(s) 369
SacI GAGCTC 1 cut(s) 289
SapI GCTCTTC 1 cut(s) 271
SaqAI TTAA 4 cut(s) 48, 171, 500, 562
Sau3AI GATC 2 cut(s) 238, 418
ScrFI CCNGG 1 cut(s) 236
SduI GDGCHC 1 cut(s) 289
SgrAI CRCCGGYG 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 4 cut(s) 107, 288, 323, 552
SmoI CTYRAG 4 cut(s) 107, 288, 323, 552
SsiI CCGC 1 cut(s) 355
SstI GAGCTC 1 cut(s) 289
StyD4I CCNGG 1 cut(s) 234
TaaI ACNGT 3 cut(s) 250, 371, 505
TaqI TCGA 1 cut(s) 377
Tru1I TTAA 4 cut(s) 48, 171, 500, 562
Tru9I TTAA 4 cut(s) 48, 171, 500, 562
TseFI GTSAC 2 cut(s) 250, 469
Tsp45I GTSAC 2 cut(s) 250, 469
TspDTI ATGAA 1 cut(s) 8
Tth111I GACNNNGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.