Rroxscaffold_2G00144740

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014634
Physical Location & Seq
Reverse (-)
82653884 .. 82654438
555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00144740.1

Sequence Viewer

Length: 555 bp
ATGGCTCTCTCTGTGGTTCATGTTCTACTTCTTGCCCTCCTCTTAGCTAAAGCCACCTCTACCTCTCACCACCACCACCATCATCACCACGAAAAACTCAAGTCCCTCCACTTTGCACTCTTTCAACATGAGACCATTAACAAAACCGGATACATTGTAGTTAATGGCGTAGCCGGAACAGCTGTTAGTCAAACCACAACGCCTTTCGGCACCCTGTTTGCTTTCCAGGATCCGATGACTGTCACAGCCAATCGAACTTCAAAGGTAGCTGGGATAGCTCAAGGAACTTCCATCACTTCCAGCTTGGATGGGCTTGAGAGCATTTCAATTGCTAAGATAACCTTGCGGTTGAAGCACTACAGTGGTTCGATTTCCATTGTTGGAGGCACACATAATATCAAGCCTGCTGATCATCCTGTTGTCGGAGGCACCGGTGATTTCAAGCTTGTGCAGGGCTATGTGACATCATCTCCGGTCAATCTCATGGGCTTAACTGTTACCTACAAGATTGCATTCCACCTTTACTGGCCTCCATATGCAACTCAAGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

184

Amino Acids

19.7

Weight (kDa)

9.52

Isoelectric Point (pI)

17.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 37 - 162 3.4e-26 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10500
malus_domestica MD02G1118100.v1.1 MD02G1118400.v1.1
prunus_persica Prupe.7G181300_v2.0.a1
pyrus_communis pycom02g09170 pycom02g09180 pycom15g20710
rosa_chinensis RchiOBHm_Chr2g0097361
rosa_laevigata RLG00000016720
rosa_multiflora Rmu_sc0001060.1_g000010
rosa_roxburghii Rroxscaffold_2G00144740
rosa_rugosa Rorug02G0066000
rosa_samantha Rh2AG112800 Rh2BG115600 Rh2CG117800 Rh2DG116800
rosa_wichuraiana Rw2G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 209, 428
AciI CCGC 1 cut(s) 346
AclWI GGATC 2 cut(s) 224, 237
AfiI CCNNNNNNNGG 1 cut(s) 422
AgeI ACCGGT 1 cut(s) 431
AgsI TTSAA 5 cut(s) 125, 261, 327, 352, 442
AjnI CCWGG 1 cut(s) 225
AleI CACNNNNGTG 1 cut(s) 360
AluBI AGCT 7 cut(s) 47, 182, 269, 278, 303, 445, 548
AluI AGCT 7 cut(s) 47, 182, 269, 278, 303, 445, 548
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 2 cut(s) 224, 237
AoxI GGCC 1 cut(s) 527
AsiGI ACCGGT 1 cut(s) 431
AsuHPI GGTGA 3 cut(s) 59, 77, 446
BamHI GGATCC 1 cut(s) 229
BanI GGYRCC 2 cut(s) 209, 428
BccI CCATC 3 cut(s) 87, 299, 302
BciT130I CCWGG 1 cut(s) 227
BciVI GTATCC 1 cut(s) 143
BclI TGATCA 1 cut(s) 409
BcoDI GTCTC 1 cut(s) 125
BfmI CTRYAG 1 cut(s) 358
BfuI GTATCC 1 cut(s) 143
Bme1390I CCNGG 1 cut(s) 227
BmiI GGNNCC 3 cut(s) 211, 231, 430
BmrFI CCNGG 1 cut(s) 227
BpuEI CTTGAG 4 cut(s) 83, 264, 335, 528
BsaI GGTCTC 1 cut(s) 125
BsaWI WCCGGW 3 cut(s) 146, 431, 472
BsaXI ACNNNNNCTCC 2 cut(s) 454, 484
Bsc4I CCNNNNNNNGG 1 cut(s) 422
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 1 cut(s) 530
BseBI CCWGG 1 cut(s) 227
BseGI GGATG 2 cut(s) 313, 412
BseLI CCNNNNNNNGG 1 cut(s) 422
BseNI ACTGG 1 cut(s) 530
BseRI GAGGAG 1 cut(s) 29
BseYI CCCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 470
BshFI GGCC 1 cut(s) 529
BshNI GGYRCC 2 cut(s) 209, 428
BshTI ACCGGT 1 cut(s) 431
BsiSI CCGG 4 cut(s) 147, 174, 432, 473
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 1 cut(s) 422
BsmAI GTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 88
BsmI GAATGC 1 cut(s) 512
BsnI GGCC 1 cut(s) 529
Bso31I GGTCTC 1 cut(s) 125
Bsp143I GATC 2 cut(s) 229, 409
BspACI CCGC 1 cut(s) 346
BspANI GGCC 1 cut(s) 529
BspLI GGNNCC 3 cut(s) 211, 231, 430
BspPI GGATC 2 cut(s) 224, 237
BspT107I GGYRCC 2 cut(s) 209, 428
BspTNI GGTCTC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 1 cut(s) 530
BssAI RCCGGY 1 cut(s) 431
BssMI GATC 2 cut(s) 229, 409
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 3 cut(s) 241, 362, 496
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 2 cut(s) 43, 333
BstF5I GGATG 2 cut(s) 313, 412
BstKTI GATC 2 cut(s) 232, 412
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 2 cut(s) 229, 409
BstMWI GCNNNNNNNGC 4 cut(s) 179, 275, 352, 545
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstSFI CTRYAG 1 cut(s) 358
BstX2I RGATCY 1 cut(s) 229
BstYI RGATCY 1 cut(s) 229
BsuI GTATCC 1 cut(s) 143
BsuRI GGCC 1 cut(s) 529
BtsCI GGATG 2 cut(s) 313, 412
BtsIMutI CAGTG 1 cut(s) 367
Cac8I GCNNGC 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 431
CspAI ACCGGT 1 cut(s) 431
CviAII CATG 3 cut(s) 20, 128, 484
DdeI CTNAG 2 cut(s) 43, 333
DpnI GATC 2 cut(s) 231, 411
DpnII GATC 2 cut(s) 229, 409
Eco31I GGTCTC 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 225
FaeI CATG 3 cut(s) 23, 131, 487
FaiI YATR 7 cut(s) 21, 129, 393, 459, 485, 535, 537
FalI AAGNNNNNCTT 2 cut(s) 326, 358
FaqI GGGAC 1 cut(s) 88
FatI CATG 3 cut(s) 19, 127, 483
FauNDI CATATG 1 cut(s) 535
FbaI TGATCA 1 cut(s) 409
FokI GGATG 2 cut(s) 320, 399
GsaI CCCAGC 1 cut(s) 273
HaeIII GGCC 1 cut(s) 529
HapII CCGG 4 cut(s) 147, 174, 432, 473
Hin1II CATG 3 cut(s) 23, 131, 487
HindIII AAGCTT 2 cut(s) 443, 546
HpaII CCGG 4 cut(s) 147, 174, 432, 473
HphI GGTGA 3 cut(s) 59, 77, 446
Hpy188I TCNGA 2 cut(s) 234, 425
HpyCH4III ACNGT 3 cut(s) 241, 362, 496
HpyCH4V TGCA 4 cut(s) 116, 451, 512, 539
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 275, 352, 545
HpyF3I CTNAG 2 cut(s) 43, 333
Hsp92II CATG 3 cut(s) 23, 131, 487
Ksp22I TGATCA 1 cut(s) 409
Kzo9I GATC 2 cut(s) 229, 409
MaeIII GTNAC 3 cut(s) 241, 460, 496
MalI GATC 2 cut(s) 231, 411
MboI GATC 2 cut(s) 229, 409
MfeI CAATTG 1 cut(s) 327
MflI RGATCY 1 cut(s) 229
MluCI AATT 1 cut(s) 327
MmeI TCCRAC 2 cut(s) 361, 403
MnlI CCTC 8 cut(s) 47, 50, 67, 73, 116, 377, 419, 540
MseI TTAA 4 cut(s) 138, 162, 491, 553
MslI CAYNNNNRTG 1 cut(s) 360
MspA1I CMGCKG 1 cut(s) 182
MspI CCGG 4 cut(s) 147, 174, 432, 473
MspR9I CCNGG 1 cut(s) 227
MunI CAATTG 1 cut(s) 327
Mva1269I GAATGC 1 cut(s) 512
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 4 cut(s) 179, 275, 352, 545
NdeI CATATG 1 cut(s) 535
NdeII GATC 2 cut(s) 229, 409
NlaIII CATG 3 cut(s) 23, 131, 487
NlaIV GGNNCC 3 cut(s) 211, 231, 430
NmuCI GTSAC 2 cut(s) 241, 460
OliI CACNNNNGTG 1 cut(s) 360
PctI GAATGC 1 cut(s) 512
PfoI TCCNGGA 1 cut(s) 225
PinAI ACCGGT 1 cut(s) 431
Psp6I CCWGG 1 cut(s) 225
PspFI CCCAGC 1 cut(s) 269
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 3 cut(s) 211, 231, 430
PsuI RGATCY 1 cut(s) 229
PvuII CAGCTG 1 cut(s) 182
RseI CAYNNNNRTG 1 cut(s) 360
SaqAI TTAA 4 cut(s) 138, 162, 491, 553
Sau3AI GATC 2 cut(s) 229, 409
ScrFI CCNGG 1 cut(s) 227
SfcI CTRYAG 1 cut(s) 358
SgrAI CRCCGGYG 1 cut(s) 431
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 4 cut(s) 98, 279, 314, 543
SmoI CTYRAG 4 cut(s) 98, 279, 314, 543
Sse9I AATT 1 cut(s) 327
SsiI CCGC 1 cut(s) 346
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 3 cut(s) 241, 362, 496
TaqI TCGA 2 cut(s) 253, 368
TasI AATT 1 cut(s) 327
Tru1I TTAA 4 cut(s) 138, 162, 491, 553
Tru9I TTAA 4 cut(s) 138, 162, 491, 553
TscAI CASTG 1 cut(s) 367
TseFI GTSAC 2 cut(s) 241, 460
Tsp45I GTSAC 2 cut(s) 241, 460
TspDTI ATGAA 1 cut(s) 8
TspRI CASTG 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.