pycom02g09170

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014634
Physical Location & Seq
Forward (+)
6320247 .. 6321028
782 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g09170.1

Sequence Viewer

Length: 687 bp
ATGGTCTTGATCAAAACCCCAAAATATGTGTTTTGTCGATCGGACGACTCCACGCTAGAACTAGACTTTCATGTAAAATTCAGCGTTCTCCAAGTTCTTCCTGCTAGAACTGCCATGGCTCTCGCTCTGCGATTCGATATAGTTCTTCTTACGGTTCTACTTTCCGCCCTCCTCCTAACTAAAGCCACCTCTACCTCTCATCGCCACCATGACCGTCACCACCATCATCTCAAGTCCCTCCACTTCACTCTCTTCCAACACGAAACCATAAACAGAACCGGATATATTGTAGTGAATGGTGTAACAGGACCAGGCGTAAGTCAAACCACTACCCCTTTTGGCACCGTGTTTGTTTTCCAGGATCCGATGACTTTCACAGCCAATCGATCTTCGAAAGCAGTTGGGATAGCTCAAGGAACTTCTGTCACATCCAGCTTAGATGGACTTCAGAGCATTTCAGTAGCTAAGATCACTTTGCGCTTGAAGAACCACTCTGGTTCAGTTTCAATAGTCGGCGGCACGCATAATGTGAAGCCCGCCGATCATCCTGTTGTGGGAGGCACCGGCGATTTCCTGTTTGTTCAAGGCTACGTGACATCGTCCCCGGTCGATCTCAGGGGCCTAACTGTTGTCTACAAGATTGCATTTCACCTTTACTGGCCTCCATATGCAACTCAAGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

229

Amino Acids

24.87

Weight (kDa)

9.67

Isoelectric Point (pI)

25.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 81 - 214 1.8e-27 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10500
malus_domestica MD02G1118100.v1.1 MD02G1118400.v1.1
prunus_persica Prupe.7G181300_v2.0.a1
pyrus_communis pycom02g09170 pycom02g09180 pycom15g20710
rosa_chinensis RchiOBHm_Chr2g0097361
rosa_laevigata RLG00000016720
rosa_multiflora Rmu_sc0001060.1_g000010
rosa_roxburghii Rroxscaffold_2G00144740
rosa_rugosa Rorug02G0066000
rosa_samantha Rh2AG112800 Rh2BG115600 Rh2CG117800 Rh2DG116800
rosa_wichuraiana Rw2G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 341, 560
AccI GTMKAC 1 cut(s) 633
AciI CCGC 3 cut(s) 165, 516, 537
AclWI GGATC 2 cut(s) 356, 369
AcsI RAATTY 1 cut(s) 77
AcuI CTGAAG 1 cut(s) 431
AfiI CCNNNNNNNGG 1 cut(s) 554
AgsI TTSAA 3 cut(s) 484, 507, 584
AjnI CCWGG 2 cut(s) 310, 357
AluBI AGCT 4 cut(s) 410, 435, 464, 680
AluI AGCT 4 cut(s) 410, 435, 464, 680
AlwI GGATC 2 cut(s) 356, 369
AoxI GGCC 2 cut(s) 619, 659
ApoI RAATTY 1 cut(s) 77
AspLEI GCGC 1 cut(s) 480
AspS9I GGNCC 2 cut(s) 308, 619
AsuC2I CCSGG 1 cut(s) 605
AsuHPI GGTGA 2 cut(s) 209, 641
AsuII TTCGAA 1 cut(s) 392
AvaII GGWCC 1 cut(s) 308
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 2 cut(s) 341, 560
BccI CCATC 2 cut(s) 231, 434
BciT130I CCWGG 2 cut(s) 312, 359
BclI TGATCA 1 cut(s) 9
BcnI CCSGG 1 cut(s) 605
BfaI CTAG 3 cut(s) 56, 62, 105
BisI GCNGC 1 cut(s) 517
BlsI GCNGC 1 cut(s) 518
Bme1390I CCNGG 3 cut(s) 312, 359, 605
Bme18I GGWCC 1 cut(s) 308
BmgT120I GGNCC 2 cut(s) 308, 619
BmiI GGNNCC 4 cut(s) 343, 363, 562, 620
BmrFI CCNGG 3 cut(s) 312, 359, 605
Bpu14I TTCGAA 1 cut(s) 392
BpuEI CTTGAG 3 cut(s) 215, 396, 660
BpuMI CCSGG 1 cut(s) 605
Bsa29I ATCGAT 1 cut(s) 385
BsaAI YACGTR 1 cut(s) 592
BsaJI CCNNGG 2 cut(s) 114, 603
BsaWI WCCGGW 1 cut(s) 278
Bsc4I CCNNNNNNNGG 1 cut(s) 554
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 1 cut(s) 662
BseBI CCWGG 2 cut(s) 312, 359
BseCI ATCGAT 1 cut(s) 385
BseDI CCNNGG 2 cut(s) 114, 603
BseGI GGATG 2 cut(s) 428, 544
BseLI CCNNNNNNNGG 1 cut(s) 554
BseMII CTCAG 1 cut(s) 628
BseNI ACTGG 1 cut(s) 662
BseRI GAGGAG 1 cut(s) 161
Bsh1285I CGRYCG 2 cut(s) 41, 609
BshFI GGCC 2 cut(s) 621, 661
BshNI GGYRCC 2 cut(s) 341, 560
BshVI ATCGAT 1 cut(s) 385
BsiEI CGRYCG 2 cut(s) 41, 609
BsiSI CCGG 3 cut(s) 279, 564, 605
BslFI GGGAC 2 cut(s) 220, 586
BslI CCNNNNNNNGG 1 cut(s) 554
BsmFI GGGAC 2 cut(s) 220, 586
BsnI GGCC 2 cut(s) 621, 661
Bsp119I TTCGAA 1 cut(s) 392
Bsp143I GATC 7 cut(s) 9, 38, 361, 386, 468, 541, 610
Bsp19I CCATGG 1 cut(s) 114
BspACI CCGC 3 cut(s) 165, 516, 537
BspANI GGCC 2 cut(s) 621, 661
BspCNI CTCAG 1 cut(s) 627
BspDI ATCGAT 1 cut(s) 385
BspLI GGNNCC 4 cut(s) 343, 363, 562, 620
BspPI GGATC 2 cut(s) 356, 369
BspT104I TTCGAA 1 cut(s) 392
BspT107I GGYRCC 2 cut(s) 341, 560
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 1 cut(s) 662
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 2 cut(s) 114, 603
BssMI GATC 7 cut(s) 9, 38, 361, 386, 468, 541, 610
BssT1I CCWWGG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 312, 359
Bst4CI ACNGT 4 cut(s) 154, 215, 346, 628
Bst6I CTCTTC 1 cut(s) 257
BstBAI YACGTR 1 cut(s) 592
BstBI TTCGAA 1 cut(s) 392
BstC8I GCNNGC 2 cut(s) 521, 537
BstDEI CTNAG 3 cut(s) 436, 465, 614
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 2 cut(s) 428, 544
BstHHI GCGC 1 cut(s) 480
BstKTI GATC 7 cut(s) 12, 41, 364, 389, 471, 544, 613
BstMBI GATC 7 cut(s) 9, 38, 361, 386, 468, 541, 610
BstMCI CGRYCG 2 cut(s) 41, 609
BstMWI GCNNNNNNNGC 2 cut(s) 110, 677
BstNI CCWGG 2 cut(s) 312, 359
BstSCI CCNGG 3 cut(s) 310, 357, 603
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
Bsu15I ATCGAT 1 cut(s) 385
BsuRI GGCC 2 cut(s) 621, 661
BsuTUI ATCGAT 1 cut(s) 385
BtgI CCRYGG 1 cut(s) 114
BtgZI GCGATG 1 cut(s) 185
BtsCI GGATG 2 cut(s) 428, 544
Cac8I GCNNGC 2 cut(s) 521, 537
CfoI GCGC 1 cut(s) 480
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 2 cut(s) 308, 619
ClaI ATCGAT 1 cut(s) 385
CviAII CATG 3 cut(s) 71, 115, 209
DdeI CTNAG 3 cut(s) 436, 465, 614
DpnI GATC 7 cut(s) 11, 40, 363, 388, 470, 543, 612
DpnII GATC 7 cut(s) 9, 38, 361, 386, 468, 541, 610
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
EciI GGCGGA 1 cut(s) 154
Eco130I CCWWGG 1 cut(s) 114
Eco47I GGWCC 1 cut(s) 308
Eco57I CTGAAG 1 cut(s) 431
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 2 cut(s) 310, 357
EcoT14I CCWWGG 1 cut(s) 114
ErhI CCWWGG 1 cut(s) 114
FaeI CATG 3 cut(s) 74, 118, 212
FaqI GGGAC 2 cut(s) 220, 586
FatI CATG 3 cut(s) 70, 114, 208
FauI CCCGC 1 cut(s) 544
FauNDI CATATG 1 cut(s) 667
FbaI TGATCA 1 cut(s) 9
FblI GTMKAC 1 cut(s) 633
Fnu4HI GCNGC 1 cut(s) 517
FokI GGATG 2 cut(s) 415, 531
Fsp4HI GCNGC 1 cut(s) 517
FspBI CTAG 3 cut(s) 56, 62, 105
GlaI GCGC 1 cut(s) 479
GluI GCNGC 1 cut(s) 517
HaeIII GGCC 2 cut(s) 621, 661
HapII CCGG 3 cut(s) 279, 564, 605
HhaI GCGC 1 cut(s) 480
Hin1II CATG 3 cut(s) 74, 118, 212
Hin6I GCGC 1 cut(s) 478
HinP1I GCGC 1 cut(s) 478
HindIII AAGCTT 1 cut(s) 678
HinfI GANTC 2 cut(s) 47, 132
HpaII CCGG 3 cut(s) 279, 564, 605
HphI GGTGA 2 cut(s) 209, 641
Hpy166II GTNNAC 1 cut(s) 634
Hpy188I TCNGA 3 cut(s) 43, 366, 450
Hpy188III TCNNGA 1 cut(s) 7
Hpy8I GTNNAC 1 cut(s) 634
HpyCH4III ACNGT 4 cut(s) 154, 215, 346, 628
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 2 cut(s) 644, 671
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 677
HpyF3I CTNAG 3 cut(s) 436, 465, 614
HpySE526I ACGT 1 cut(s) 591
Hsp92II CATG 3 cut(s) 74, 118, 212
HspAI GCGC 1 cut(s) 478
Ksp22I TGATCA 1 cut(s) 9
Kzo9I GATC 7 cut(s) 9, 38, 361, 386, 468, 541, 610
MaeI CTAG 3 cut(s) 56, 62, 105
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 4 cut(s) 215, 301, 424, 592
MalI GATC 7 cut(s) 11, 40, 363, 388, 470, 543, 612
MboI GATC 7 cut(s) 9, 38, 361, 386, 468, 541, 610
MboII GAAGA 5 cut(s) 89, 137, 244, 381, 496
MflI RGATCY 1 cut(s) 361
MluCI AATT 1 cut(s) 77
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 280
MnlI CCTC 7 cut(s) 179, 182, 199, 205, 248, 551, 672
MseI TTAA 1 cut(s) 685
MspI CCGG 3 cut(s) 279, 564, 605
MspR9I CCNGG 3 cut(s) 312, 359, 605
MvaI CCWGG 2 cut(s) 312, 359
MwoI GCNNNNNNNGC 2 cut(s) 110, 677
NciI CCSGG 1 cut(s) 605
NcoI CCATGG 1 cut(s) 114
NdeI CATATG 1 cut(s) 667
NdeII GATC 7 cut(s) 9, 38, 361, 386, 468, 541, 610
NlaIII CATG 3 cut(s) 74, 118, 212
NlaIV GGNNCC 4 cut(s) 343, 363, 562, 620
NmuCI GTSAC 3 cut(s) 215, 424, 592
NspV TTCGAA 1 cut(s) 392
PfeI GAWTC 1 cut(s) 132
PflFI GACNNNGTC 1 cut(s) 598
PfoI TCCNGGA 1 cut(s) 357
PkrI GCNGC 1 cut(s) 518
Ple19I CGATCG 1 cut(s) 41
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
Ppu21I YACGTR 1 cut(s) 592
Psp6I CCWGG 2 cut(s) 310, 357
PspGI CCWGG 2 cut(s) 310, 357
PspN4I GGNNCC 4 cut(s) 343, 363, 562, 620
PspPI GGNCC 2 cut(s) 308, 619
PsuI RGATCY 1 cut(s) 361
PsyI GACNNNGTC 1 cut(s) 598
PvuI CGATCG 1 cut(s) 41
SaqAI TTAA 1 cut(s) 685
SatI GCNGC 1 cut(s) 517
Sau3AI GATC 7 cut(s) 9, 38, 361, 386, 468, 541, 610
Sau96I GGNCC 2 cut(s) 308, 619
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 3 cut(s) 312, 359, 605
SetI ASST 8 cut(s) 191, 197, 412, 437, 466, 594, 654, 682
SfuI TTCGAA 1 cut(s) 392
SgrAI CRCCGGYG 1 cut(s) 563
SinI GGWCC 1 cut(s) 308
SmlI CTYRAG 3 cut(s) 230, 411, 675
SmoI CTYRAG 3 cut(s) 230, 411, 675
Sse9I AATT 1 cut(s) 77
SsiI CCGC 3 cut(s) 165, 516, 537
SspMI CTAG 3 cut(s) 56, 62, 105
StyD4I CCNGG 3 cut(s) 310, 357, 603
StyI CCWWGG 1 cut(s) 114
TaaI ACNGT 4 cut(s) 154, 215, 346, 628
TaiI ACGT 1 cut(s) 594
TaqI TCGA 5 cut(s) 37, 135, 385, 392, 609
TasI AATT 1 cut(s) 77
TauI GCSGC 1 cut(s) 519
TfiI GAWTC 1 cut(s) 132
Tru1I TTAA 1 cut(s) 685
Tru9I TTAA 1 cut(s) 685
TseFI GTSAC 3 cut(s) 215, 424, 592
Tsp45I GTSAC 3 cut(s) 215, 424, 592
TspDTI ATGAA 1 cut(s) 59
Tth111I GACNNNGTC 1 cut(s) 598
VpaK11BI GGWCC 1 cut(s) 308
XapI RAATTY 1 cut(s) 77
XmiI GTMKAC 1 cut(s) 633
XspI CTAG 3 cut(s) 56, 62, 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.