RchiOBHm_Chr2g0097361

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014634
Physical Location & Seq
Forward (+)
10015366 .. 10016056
691 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47223

Sequence Viewer

Length: 570 bp
ATGGCTCTCTCTGTGGTTCATGTTCTACTTCTTGCCCTCCTCTTAGCTAAAGCCACCTCTACCTCTCACCACCACCACCACCACCACCACCATCATCATCACCACGAAAAACTCAAGTCCCTCCACTTTGCACTCTTTCAACATGAGACCATTAACAAAACCGGATACATTGTAGTTAATGGTGTAGCCGGAACGGCTGTTAGTCAAACCACAACGCCTTTCGGCACCCTGTTTGCTTTCCAGGATCCGATGACTGTCACAGCCAATCGATCTTCAAAGGTAGCTGGGATAGCTCAAGGAACTTCCATCACTTCCAGCTTGGATGGGCTTGAGAGCATTTCAATTGCTAAGATAACCTTGCGGTTGAAGCACTACAGTGGTTCGATTTCCATTGTTGGTGGCACACATAATATCAAGCCTGCTGATCATCCTGTTGTCGGAGGCACCGGTGATTTCAAGCTTGTGCAGGGCTATGTGACATCATCTCCGGTCAATCTCATGGGCTTAACTGTTACCTACAAGATTGCATTCCACCTTTACTGGCCTCCATATGCAACTCAAGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

189

Amino Acids

20.37

Weight (kDa)

9.52

Isoelectric Point (pI)

20.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 167 3.4e-26 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10500
malus_domestica MD02G1118100.v1.1 MD02G1118400.v1.1
prunus_persica Prupe.7G181300_v2.0.a1
pyrus_communis pycom02g09170 pycom02g09180 pycom15g20710
rosa_chinensis RchiOBHm_Chr2g0097361
rosa_laevigata RLG00000016720
rosa_multiflora Rmu_sc0001060.1_g000010
rosa_roxburghii Rroxscaffold_2G00144740
rosa_rugosa Rorug02G0066000
rosa_samantha Rh2AG112800 Rh2BG115600 Rh2CG117800 Rh2DG116800
rosa_wichuraiana Rw2G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 224, 443
AciI CCGC 1 cut(s) 361
AclWI GGATC 2 cut(s) 239, 252
AfiI CCNNNNNNNGG 1 cut(s) 437
AgeI ACCGGT 1 cut(s) 446
AgsI TTSAA 5 cut(s) 140, 276, 342, 367, 457
AjnI CCWGG 1 cut(s) 240
AleI CACNNNNGTG 1 cut(s) 375
AluBI AGCT 6 cut(s) 47, 284, 293, 318, 460, 563
AluI AGCT 6 cut(s) 47, 284, 293, 318, 460, 563
Alw26I GTCTC 1 cut(s) 140
AlwI GGATC 2 cut(s) 239, 252
AoxI GGCC 1 cut(s) 542
AsiGI ACCGGT 1 cut(s) 446
AsuHPI GGTGA 3 cut(s) 59, 92, 461
BamHI GGATCC 1 cut(s) 244
BanI GGYRCC 2 cut(s) 224, 443
BccI CCATC 3 cut(s) 99, 314, 317
BceAI ACGGC 1 cut(s) 210
BciT130I CCWGG 1 cut(s) 242
BciVI GTATCC 1 cut(s) 158
BclI TGATCA 1 cut(s) 424
BcoDI GTCTC 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 373
BfuI GTATCC 1 cut(s) 158
BglI GCCNNNNNGGC 1 cut(s) 194
Bme1390I CCNGG 1 cut(s) 242
BmiI GGNNCC 3 cut(s) 226, 246, 445
BmrFI CCNGG 1 cut(s) 242
BpuEI CTTGAG 4 cut(s) 98, 279, 350, 543
Bsa29I ATCGAT 1 cut(s) 268
BsaI GGTCTC 1 cut(s) 140
BsaWI WCCGGW 3 cut(s) 161, 446, 487
BsaXI ACNNNNNCTCC 2 cut(s) 469, 499
Bsc4I CCNNNNNNNGG 1 cut(s) 437
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 1 cut(s) 545
BseBI CCWGG 1 cut(s) 242
BseCI ATCGAT 1 cut(s) 268
BseGI GGATG 2 cut(s) 328, 427
BseLI CCNNNNNNNGG 1 cut(s) 437
BseNI ACTGG 1 cut(s) 545
BseRI GAGGAG 1 cut(s) 29
BseYI CCCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 485
BshFI GGCC 1 cut(s) 544
BshNI GGYRCC 2 cut(s) 224, 443
BshTI ACCGGT 1 cut(s) 446
BshVI ATCGAT 1 cut(s) 268
BsiSI CCGG 4 cut(s) 162, 189, 447, 488
BslFI GGGAC 1 cut(s) 103
BslI CCNNNNNNNGG 1 cut(s) 437
BsmAI GTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 103
BsmI GAATGC 1 cut(s) 527
BsnI GGCC 1 cut(s) 544
Bso31I GGTCTC 1 cut(s) 140
Bsp143I GATC 3 cut(s) 244, 269, 424
BspACI CCGC 1 cut(s) 361
BspANI GGCC 1 cut(s) 544
BspDI ATCGAT 1 cut(s) 268
BspLI GGNNCC 3 cut(s) 226, 246, 445
BspPI GGATC 2 cut(s) 239, 252
BspT107I GGYRCC 2 cut(s) 224, 443
BspTNI GGTCTC 1 cut(s) 140
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 1 cut(s) 545
BssAI RCCGGY 1 cut(s) 446
BssMI GATC 3 cut(s) 244, 269, 424
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 3 cut(s) 256, 377, 511
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 2 cut(s) 43, 348
BstF5I GGATG 2 cut(s) 328, 427
BstKTI GATC 3 cut(s) 247, 272, 427
BstMAI GTCTC 1 cut(s) 140
BstMBI GATC 3 cut(s) 244, 269, 424
BstMWI GCNNNNNNNGC 4 cut(s) 194, 290, 367, 560
BstNI CCWGG 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 240
BstSFI CTRYAG 1 cut(s) 373
BstX2I RGATCY 1 cut(s) 244
BstYI RGATCY 1 cut(s) 244
Bsu15I ATCGAT 1 cut(s) 268
BsuI GTATCC 1 cut(s) 158
BsuRI GGCC 1 cut(s) 544
BsuTUI ATCGAT 1 cut(s) 268
BtsCI GGATG 2 cut(s) 328, 427
BtsIMutI CAGTG 1 cut(s) 382
Cac8I GCNNGC 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 446
ClaI ATCGAT 1 cut(s) 268
CspAI ACCGGT 1 cut(s) 446
CviAII CATG 3 cut(s) 20, 143, 499
DdeI CTNAG 2 cut(s) 43, 348
DpnI GATC 3 cut(s) 246, 271, 426
DpnII GATC 3 cut(s) 244, 269, 424
Eco31I GGTCTC 1 cut(s) 140
EcoRII CCWGG 1 cut(s) 240
FaeI CATG 3 cut(s) 23, 146, 502
FaiI YATR 7 cut(s) 21, 144, 408, 474, 500, 550, 552
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 1 cut(s) 103
FatI CATG 3 cut(s) 19, 142, 498
FauNDI CATATG 1 cut(s) 550
FbaI TGATCA 1 cut(s) 424
FokI GGATG 2 cut(s) 335, 414
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 1 cut(s) 544
HapII CCGG 4 cut(s) 162, 189, 447, 488
Hin1II CATG 3 cut(s) 23, 146, 502
HindIII AAGCTT 2 cut(s) 458, 561
HpaII CCGG 4 cut(s) 162, 189, 447, 488
HphI GGTGA 3 cut(s) 59, 92, 461
Hpy188I TCNGA 2 cut(s) 249, 440
HpyCH4III ACNGT 3 cut(s) 256, 377, 511
HpyCH4V TGCA 4 cut(s) 131, 466, 527, 554
HpyF10VI GCNNNNNNNGC 4 cut(s) 194, 290, 367, 560
HpyF3I CTNAG 2 cut(s) 43, 348
Hsp92II CATG 3 cut(s) 23, 146, 502
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 3 cut(s) 244, 269, 424
MaeIII GTNAC 3 cut(s) 256, 475, 511
MalI GATC 3 cut(s) 246, 271, 426
MboI GATC 3 cut(s) 244, 269, 424
MboII GAAGA 1 cut(s) 264
MfeI CAATTG 1 cut(s) 342
MflI RGATCY 1 cut(s) 244
MluCI AATT 1 cut(s) 342
MmeI TCCRAC 1 cut(s) 418
MnlI CCTC 7 cut(s) 47, 50, 67, 73, 131, 434, 555
MseI TTAA 4 cut(s) 153, 177, 506, 568
MslI CAYNNNNRTG 1 cut(s) 375
MspI CCGG 4 cut(s) 162, 189, 447, 488
MspR9I CCNGG 1 cut(s) 242
MunI CAATTG 1 cut(s) 342
Mva1269I GAATGC 1 cut(s) 527
MvaI CCWGG 1 cut(s) 242
MwoI GCNNNNNNNGC 4 cut(s) 194, 290, 367, 560
NdeI CATATG 1 cut(s) 550
NdeII GATC 3 cut(s) 244, 269, 424
NlaIII CATG 3 cut(s) 23, 146, 502
NlaIV GGNNCC 3 cut(s) 226, 246, 445
NmuCI GTSAC 2 cut(s) 256, 475
OliI CACNNNNGTG 1 cut(s) 375
PctI GAATGC 1 cut(s) 527
PfoI TCCNGGA 1 cut(s) 240
PinAI ACCGGT 1 cut(s) 446
Psp6I CCWGG 1 cut(s) 240
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 1 cut(s) 240
PspN4I GGNNCC 3 cut(s) 226, 246, 445
PsuI RGATCY 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 4 cut(s) 153, 177, 506, 568
Sau3AI GATC 3 cut(s) 244, 269, 424
ScrFI CCNGG 1 cut(s) 242
SfcI CTRYAG 1 cut(s) 373
SgrAI CRCCGGYG 1 cut(s) 446
SmiMI CAYNNNNRTG 1 cut(s) 375
SmlI CTYRAG 4 cut(s) 113, 294, 329, 558
SmoI CTYRAG 4 cut(s) 113, 294, 329, 558
Sse9I AATT 1 cut(s) 342
SsiI CCGC 1 cut(s) 361
StyD4I CCNGG 1 cut(s) 240
TaaI ACNGT 3 cut(s) 256, 377, 511
TaqI TCGA 2 cut(s) 268, 383
TasI AATT 1 cut(s) 342
Tru1I TTAA 4 cut(s) 153, 177, 506, 568
Tru9I TTAA 4 cut(s) 153, 177, 506, 568
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 2 cut(s) 256, 475
Tsp45I GTSAC 2 cut(s) 256, 475
TspDTI ATGAA 1 cut(s) 8
TspRI CASTG 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.