RLG00000016720

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014634
Physical Location & Seq
Forward (+)
9388684 .. 9389247
564 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016720

Sequence Viewer

Length: 564 bp
ATGGCTCTCTCTGTGGTTCATGTTCTACTTCTTGCCCTCCTCTTAGCTAAAGCCACCTCTACCTCTCACCACCACCACCACCACCATCATCACCACCACGAAAAACTCAAGTCCCTCCACTTTGCACTCTTTCAACATGAGACCATTAACAAAACCGGATACATTGTAGTTAATGGCGTAGCCGGAACAGCTGTTAGTCAAACCACAACGCCTTTCGGCACCCTGTTTGCTTTCCAGGATCCGATGACTGTCACAGCCAATCGATCTTCAAAGGTAGCTGGGATAGCTCAAGGAACTTCCATCACTTCCAGCTTGGATGGGCTTGAGAGCATTTCAATTGCTAAGATAACCTTGCGGTTGAAGCACTACAGTGGTTCGATTTCCATTGTTGGAGGCACACATAATATCAAGCCTGCTGATCATCCTGTTGTCGGAGGCACCGGTGATTTCAAGCTTGTGCAGGGCTATGTGACTTCATCTCCGGTCAATCTCATGGGCTTAACTGTTACCTACAAGATTGCATTCCACCTTTACTGGCCTCCATATGCAACTCAAGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

188

Amino Acids

20.1

Weight (kDa)

9.52

Isoelectric Point (pI)

21.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 40 - 165 3.3e-26 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10500
malus_domestica MD02G1118100.v1.1 MD02G1118400.v1.1
prunus_persica Prupe.7G181300_v2.0.a1
pyrus_communis pycom02g09170 pycom02g09180 pycom15g20710
rosa_chinensis RchiOBHm_Chr2g0097361
rosa_laevigata RLG00000016720
rosa_multiflora Rmu_sc0001060.1_g000010
rosa_roxburghii Rroxscaffold_2G00144740
rosa_rugosa Rorug02G0066000
rosa_samantha Rh2AG112800 Rh2BG115600 Rh2CG117800 Rh2DG116800
rosa_wichuraiana Rw2G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 218, 437
AciI CCGC 1 cut(s) 355
AclWI GGATC 2 cut(s) 233, 246
AfiI CCNNNNNNNGG 1 cut(s) 431
AgeI ACCGGT 1 cut(s) 440
AgsI TTSAA 5 cut(s) 134, 270, 336, 361, 451
AjnI CCWGG 1 cut(s) 234
AleI CACNNNNGTG 1 cut(s) 369
AluBI AGCT 7 cut(s) 47, 191, 278, 287, 312, 454, 557
AluI AGCT 7 cut(s) 47, 191, 278, 287, 312, 454, 557
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 2 cut(s) 233, 246
AoxI GGCC 1 cut(s) 536
AsiGI ACCGGT 1 cut(s) 440
AsuHPI GGTGA 3 cut(s) 59, 83, 455
BamHI GGATCC 1 cut(s) 238
BanI GGYRCC 2 cut(s) 218, 437
BccI CCATC 3 cut(s) 93, 308, 311
BciT130I CCWGG 1 cut(s) 236
BciVI GTATCC 1 cut(s) 152
BclI TGATCA 1 cut(s) 418
BcoDI GTCTC 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 367
BfuI GTATCC 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 236
BmiI GGNNCC 3 cut(s) 220, 240, 439
BmrFI CCNGG 1 cut(s) 236
BpuEI CTTGAG 4 cut(s) 92, 273, 344, 537
Bsa29I ATCGAT 1 cut(s) 262
BsaI GGTCTC 1 cut(s) 134
BsaWI WCCGGW 3 cut(s) 155, 440, 481
BsaXI ACNNNNNCTCC 2 cut(s) 463, 493
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 1 cut(s) 539
BseBI CCWGG 1 cut(s) 236
BseCI ATCGAT 1 cut(s) 262
BseGI GGATG 2 cut(s) 322, 421
BseLI CCNNNNNNNGG 1 cut(s) 431
BseNI ACTGG 1 cut(s) 539
BseRI GAGGAG 1 cut(s) 29
BseYI CCCAGC 1 cut(s) 278
BsgI GTGCAG 1 cut(s) 479
BshFI GGCC 1 cut(s) 538
BshNI GGYRCC 2 cut(s) 218, 437
BshTI ACCGGT 1 cut(s) 440
BshVI ATCGAT 1 cut(s) 262
BsiSI CCGG 4 cut(s) 156, 183, 441, 482
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 97
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 1 cut(s) 538
Bso31I GGTCTC 1 cut(s) 134
Bsp143I GATC 3 cut(s) 238, 263, 418
BspACI CCGC 1 cut(s) 355
BspANI GGCC 1 cut(s) 538
BspDI ATCGAT 1 cut(s) 262
BspLI GGNNCC 3 cut(s) 220, 240, 439
BspPI GGATC 2 cut(s) 233, 246
BspT107I GGYRCC 2 cut(s) 218, 437
BspTNI GGTCTC 1 cut(s) 134
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 1 cut(s) 539
BssAI RCCGGY 1 cut(s) 440
BssMI GATC 3 cut(s) 238, 263, 418
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 3 cut(s) 250, 371, 505
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 2 cut(s) 43, 342
BstF5I GGATG 2 cut(s) 322, 421
BstKTI GATC 3 cut(s) 241, 266, 421
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 3 cut(s) 238, 263, 418
BstMWI GCNNNNNNNGC 4 cut(s) 188, 284, 361, 554
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 1 cut(s) 367
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
Bsu15I ATCGAT 1 cut(s) 262
BsuI GTATCC 1 cut(s) 152
BsuRI GGCC 1 cut(s) 538
BsuTUI ATCGAT 1 cut(s) 262
BtsCI GGATG 2 cut(s) 322, 421
BtsIMutI CAGTG 1 cut(s) 376
Cac8I GCNNGC 1 cut(s) 414
Cfr10I RCCGGY 1 cut(s) 440
ClaI ATCGAT 1 cut(s) 262
CspAI ACCGGT 1 cut(s) 440
CviAII CATG 3 cut(s) 20, 137, 493
DdeI CTNAG 2 cut(s) 43, 342
DpnI GATC 3 cut(s) 240, 265, 420
DpnII GATC 3 cut(s) 238, 263, 418
Eco31I GGTCTC 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 234
FaeI CATG 3 cut(s) 23, 140, 496
FaiI YATR 7 cut(s) 21, 138, 402, 468, 494, 544, 546
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FaqI GGGAC 1 cut(s) 97
FatI CATG 3 cut(s) 19, 136, 492
FauNDI CATATG 1 cut(s) 544
FbaI TGATCA 1 cut(s) 418
FokI GGATG 2 cut(s) 329, 408
GsaI CCCAGC 1 cut(s) 282
HaeIII GGCC 1 cut(s) 538
HapII CCGG 4 cut(s) 156, 183, 441, 482
Hin1II CATG 3 cut(s) 23, 140, 496
HindIII AAGCTT 2 cut(s) 452, 555
HpaII CCGG 4 cut(s) 156, 183, 441, 482
HphI GGTGA 3 cut(s) 59, 83, 455
Hpy188I TCNGA 2 cut(s) 243, 434
HpyCH4III ACNGT 3 cut(s) 250, 371, 505
HpyCH4V TGCA 4 cut(s) 125, 460, 521, 548
HpyF10VI GCNNNNNNNGC 4 cut(s) 188, 284, 361, 554
HpyF3I CTNAG 2 cut(s) 43, 342
Hsp92II CATG 3 cut(s) 23, 140, 496
Ksp22I TGATCA 1 cut(s) 418
Kzo9I GATC 3 cut(s) 238, 263, 418
MaeIII GTNAC 3 cut(s) 250, 469, 505
MalI GATC 3 cut(s) 240, 265, 420
MboI GATC 3 cut(s) 238, 263, 418
MboII GAAGA 1 cut(s) 258
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 1 cut(s) 238
MluCI AATT 1 cut(s) 336
MmeI TCCRAC 2 cut(s) 370, 412
MnlI CCTC 8 cut(s) 47, 50, 67, 73, 125, 386, 428, 549
MseI TTAA 4 cut(s) 147, 171, 500, 562
MslI CAYNNNNRTG 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 4 cut(s) 156, 183, 441, 482
MspR9I CCNGG 1 cut(s) 236
MunI CAATTG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 521
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 4 cut(s) 188, 284, 361, 554
NdeI CATATG 1 cut(s) 544
NdeII GATC 3 cut(s) 238, 263, 418
NlaIII CATG 3 cut(s) 23, 140, 496
NlaIV GGNNCC 3 cut(s) 220, 240, 439
NmuCI GTSAC 2 cut(s) 250, 469
OliI CACNNNNGTG 1 cut(s) 369
PctI GAATGC 1 cut(s) 521
PfoI TCCNGGA 1 cut(s) 234
PinAI ACCGGT 1 cut(s) 440
Psp6I CCWGG 1 cut(s) 234
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 3 cut(s) 220, 240, 439
PsuI RGATCY 1 cut(s) 238
PvuII CAGCTG 1 cut(s) 191
RseI CAYNNNNRTG 1 cut(s) 369
SaqAI TTAA 4 cut(s) 147, 171, 500, 562
Sau3AI GATC 3 cut(s) 238, 263, 418
ScrFI CCNGG 1 cut(s) 236
SfcI CTRYAG 1 cut(s) 367
SgrAI CRCCGGYG 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 4 cut(s) 107, 288, 323, 552
SmoI CTYRAG 4 cut(s) 107, 288, 323, 552
Sse9I AATT 1 cut(s) 336
SsiI CCGC 1 cut(s) 355
StyD4I CCNGG 1 cut(s) 234
TaaI ACNGT 3 cut(s) 250, 371, 505
TaqI TCGA 2 cut(s) 262, 377
TasI AATT 1 cut(s) 336
Tru1I TTAA 4 cut(s) 147, 171, 500, 562
Tru9I TTAA 4 cut(s) 147, 171, 500, 562
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 2 cut(s) 250, 469
Tsp45I GTSAC 2 cut(s) 250, 469
TspDTI ATGAA 2 cut(s) 8, 465
TspRI CASTG 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.