MD02G1118400.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014634
Physical Location & Seq
Forward (+)
9610808 .. 9611398
591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1118400.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGCTCTCGCTCTGCGATTCGATATAGTTCTTCTTCCGGTTCTACTTTCCGCCCTCCTCCTAACTAAAGCCGCCTCTACCTCTCATCACCACCATGACCGTCGCCACCACCATAACCGTCACCACCATCATCTCAAGTCCCTCCACTTCACTCTCTTTCAACACGAAACCAGAAACAGAACTGGATATATTGTAGTGAATGGTGTAACAGGACCAGGCGTAAGTCAAACCACTACCCCTTTTGGCACCGTGTTTGTTTTCCAGGATCCGATGACTGTCACAGCCAATCAATCTTCAAAAGCAGTTGGGATAGCTCAAGGAACTACCGTCTCATCCAGCTTAGATGGACTTCAGAGCATTTCAGTGGCTAAGATCACTTTGCGCTTGAAGAACCACTCTGGTTCAGTTTCTATAGTCAGAGACACGCATAATGTGAAGGCCACCGATCATCCCGTTGTGGGAGGCACCGGCGATTTCCTGTTTGTTCAAGGCTACGTGACATCGTCCCCTGTCGATCTCAGGGGCCTAACTGTTGTCTACAGGATTGCATTTCACCTTTACTGGCCTCCATATACAACTCAAGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

197

Amino Acids

21.51

Weight (kDa)

10.06

Isoelectric Point (pI)

26.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 49 - 182 1.4e-26 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10500
malus_domestica MD02G1118100.v1.1 MD02G1118400.v1.1
prunus_persica Prupe.7G181300_v2.0.a1
pyrus_communis pycom02g09170 pycom02g09180 pycom15g20710
rosa_chinensis RchiOBHm_Chr2g0097361
rosa_laevigata RLG00000016720
rosa_multiflora Rmu_sc0001060.1_g000010
rosa_roxburghii Rroxscaffold_2G00144740
rosa_rugosa Rorug02G0066000
rosa_samantha Rh2AG112800 Rh2BG115600 Rh2CG117800 Rh2DG116800
rosa_wichuraiana Rw2G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 245, 464
AccI GTMKAC 1 cut(s) 537
AciI CCGC 2 cut(s) 51, 72
AclWI GGATC 2 cut(s) 260, 273
AcuI CTGAAG 1 cut(s) 335
AfiI CCNNNNNNNGG 1 cut(s) 458
AgsI TTSAA 4 cut(s) 161, 297, 388, 488
AjnI CCWGG 2 cut(s) 214, 261
AluBI AGCT 2 cut(s) 314, 339
AluI AGCT 2 cut(s) 314, 339
Alw26I GTCTC 2 cut(s) 334, 414
AlwI GGATC 2 cut(s) 260, 273
AoxI GGCC 3 cut(s) 438, 523, 563
AspLEI GCGC 1 cut(s) 384
AspS9I GGNCC 2 cut(s) 212, 523
AsuHPI GGTGA 3 cut(s) 80, 113, 545
AvaII GGWCC 1 cut(s) 212
BamHI GGATCC 1 cut(s) 265
BanI GGYRCC 2 cut(s) 245, 464
BccI CCATC 2 cut(s) 135, 338
BciT130I CCWGG 2 cut(s) 216, 263
BcoDI GTCTC 2 cut(s) 334, 414
BfmI CTRYAG 2 cut(s) 411, 538
BisI GCNGC 1 cut(s) 72
BlsI GCNGC 1 cut(s) 73
Bme1390I CCNGG 2 cut(s) 216, 263
Bme18I GGWCC 1 cut(s) 212
BmgT120I GGNCC 2 cut(s) 212, 523
BmiI GGNNCC 4 cut(s) 247, 267, 466, 524
BmrFI CCNGG 2 cut(s) 216, 263
BpuEI CTTGAG 3 cut(s) 119, 300, 564
BsaAI YACGTR 1 cut(s) 496
BsaWI WCCGGW 1 cut(s) 37
Bsc4I CCNNNNNNNGG 1 cut(s) 458
Bse118I RCCGGY 1 cut(s) 467
Bse1I ACTGG 2 cut(s) 187, 566
BseBI CCWGG 2 cut(s) 216, 263
BseGI GGATG 2 cut(s) 332, 448
BseLI CCNNNNNNNGG 1 cut(s) 458
BseMII CTCAG 1 cut(s) 532
BseNI ACTGG 2 cut(s) 187, 566
BseRI GAGGAG 1 cut(s) 47
BshFI GGCC 3 cut(s) 440, 525, 565
BshNI GGYRCC 2 cut(s) 245, 464
BsiSI CCGG 2 cut(s) 38, 468
BslFI GGGAC 2 cut(s) 124, 490
BslI CCNNNNNNNGG 1 cut(s) 458
BsmAI GTCTC 2 cut(s) 334, 414
BsmBI CGTCTC 1 cut(s) 334
BsmFI GGGAC 2 cut(s) 124, 490
BsnI GGCC 3 cut(s) 440, 525, 565
Bsp143I GATC 4 cut(s) 265, 372, 445, 514
BspACI CCGC 2 cut(s) 51, 72
BspANI GGCC 3 cut(s) 440, 525, 565
BspCNI CTCAG 1 cut(s) 531
BspLI GGNNCC 4 cut(s) 247, 267, 466, 524
BspPI GGATC 2 cut(s) 260, 273
BspT107I GGYRCC 2 cut(s) 245, 464
BsrFI RCCGGY 1 cut(s) 467
BsrI ACTGG 2 cut(s) 187, 566
BssAI RCCGGY 1 cut(s) 467
BssMI GATC 4 cut(s) 265, 372, 445, 514
Bst2UI CCWGG 2 cut(s) 216, 263
Bst4CI ACNGT 6 cut(s) 101, 119, 250, 277, 328, 532
BstBAI YACGTR 1 cut(s) 496
BstDEI CTNAG 3 cut(s) 340, 369, 518
BstF5I GGATG 2 cut(s) 332, 448
BstHHI GCGC 1 cut(s) 384
BstKTI GATC 4 cut(s) 268, 375, 448, 517
BstMAI GTCTC 2 cut(s) 334, 414
BstMBI GATC 4 cut(s) 265, 372, 445, 514
BstNI CCWGG 2 cut(s) 216, 263
BstSCI CCNGG 2 cut(s) 214, 261
BstSFI CTRYAG 2 cut(s) 411, 538
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
BsuRI GGCC 3 cut(s) 440, 525, 565
BtsCI GGATG 2 cut(s) 332, 448
BtsIMutI CAGTG 1 cut(s) 369
CfoI GCGC 1 cut(s) 384
Cfr10I RCCGGY 1 cut(s) 467
Cfr13I GGNCC 2 cut(s) 212, 523
CviAII CATG 1 cut(s) 95
DdeI CTNAG 3 cut(s) 340, 369, 518
DpnI GATC 4 cut(s) 267, 374, 447, 516
DpnII GATC 4 cut(s) 265, 372, 445, 514
EciI GGCGGA 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 212
Eco57I CTGAAG 1 cut(s) 335
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 2 cut(s) 214, 261
Esp3I CGTCTC 1 cut(s) 334
FaeI CATG 1 cut(s) 98
FaiI YATR 8 cut(s) 26, 96, 114, 189, 413, 429, 571, 573
FaqI GGGAC 2 cut(s) 124, 490
FatI CATG 1 cut(s) 94
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 1 cut(s) 72
FokI GGATG 2 cut(s) 319, 435
Fsp4HI GCNGC 1 cut(s) 72
GlaI GCGC 1 cut(s) 383
GluI GCNGC 1 cut(s) 72
HaeIII GGCC 3 cut(s) 440, 525, 565
HapII CCGG 2 cut(s) 38, 468
HhaI GCGC 1 cut(s) 384
Hin1II CATG 1 cut(s) 98
Hin6I GCGC 1 cut(s) 382
HinP1I GCGC 1 cut(s) 382
HinfI GANTC 1 cut(s) 18
HpaII CCGG 2 cut(s) 38, 468
HphI GGTGA 3 cut(s) 80, 113, 545
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 3 cut(s) 270, 354, 419
Hpy8I GTNNAC 1 cut(s) 538
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 1 cut(s) 430
HpyCH4III ACNGT 6 cut(s) 101, 119, 250, 277, 328, 532
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 1 cut(s) 548
HpyF3I CTNAG 3 cut(s) 340, 369, 518
HpySE526I ACGT 1 cut(s) 495
Hsp92II CATG 1 cut(s) 98
HspAI GCGC 1 cut(s) 382
Kzo9I GATC 4 cut(s) 265, 372, 445, 514
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 4 cut(s) 119, 205, 277, 496
MalI GATC 4 cut(s) 267, 374, 447, 516
MboI GATC 4 cut(s) 265, 372, 445, 514
MboII GAAGA 4 cut(s) 23, 26, 285, 400
MflI RGATCY 1 cut(s) 265
MnlI CCTC 7 cut(s) 65, 68, 85, 91, 152, 455, 576
MseI TTAA 1 cut(s) 589
MslI CAYNNNNRTG 2 cut(s) 93, 362
MspI CCGG 2 cut(s) 38, 468
MspR9I CCNGG 2 cut(s) 216, 263
MvaI CCWGG 2 cut(s) 216, 263
NdeII GATC 4 cut(s) 265, 372, 445, 514
NlaIII CATG 1 cut(s) 98
NlaIV GGNNCC 4 cut(s) 247, 267, 466, 524
NmuCI GTSAC 3 cut(s) 119, 277, 496
PfeI GAWTC 1 cut(s) 18
PflFI GACNNNGTC 1 cut(s) 502
PfoI TCCNGGA 1 cut(s) 261
PkrI GCNGC 1 cut(s) 73
Ppu21I YACGTR 1 cut(s) 496
Psp6I CCWGG 2 cut(s) 214, 261
PspGI CCWGG 2 cut(s) 214, 261
PspN4I GGNNCC 4 cut(s) 247, 267, 466, 524
PspPI GGNCC 2 cut(s) 212, 523
PsuI RGATCY 1 cut(s) 265
PsyI GACNNNGTC 1 cut(s) 502
RseI CAYNNNNRTG 2 cut(s) 93, 362
SaqAI TTAA 1 cut(s) 589
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 4 cut(s) 265, 372, 445, 514
Sau96I GGNCC 2 cut(s) 212, 523
ScrFI CCNGG 2 cut(s) 216, 263
SetI ASST 5 cut(s) 83, 316, 341, 498, 558
SfcI CTRYAG 2 cut(s) 411, 538
SgrAI CRCCGGYG 1 cut(s) 467
SinI GGWCC 1 cut(s) 212
SmiMI CAYNNNNRTG 2 cut(s) 93, 362
SmlI CTYRAG 3 cut(s) 134, 315, 579
SmoI CTYRAG 3 cut(s) 134, 315, 579
SsiI CCGC 2 cut(s) 51, 72
StyD4I CCNGG 2 cut(s) 214, 261
TaaI ACNGT 6 cut(s) 101, 119, 250, 277, 328, 532
TaiI ACGT 1 cut(s) 498
TaqI TCGA 2 cut(s) 21, 513
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 1 cut(s) 589
Tru9I TTAA 1 cut(s) 589
TscAI CASTG 1 cut(s) 369
TseFI GTSAC 3 cut(s) 119, 277, 496
Tsp45I GTSAC 3 cut(s) 119, 277, 496
TspRI CASTG 1 cut(s) 369
Tth111I GACNNNGTC 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 212
XmiI GTMKAC 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.