MD02G1118100.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014634
Physical Location & Seq
Forward (+)
9607312 .. 9607884
573 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1118100.v1.1.491

Sequence Viewer

Length: 573 bp
ATGGCTCTCGGTCTGCGATTCGATATAGTTCTTCTTCCGGTTCTACTTTCCGCCCTCCTCCTAACTAAAGCCGCCTCTATCTCTCATCACCACCATGACCGCCACCACCATCATCTCAAGTCCCTCCACTTCACTCTCTTTCAACACGAAACCATAAACAGAACCGGATATATTGTAGTGAATGGTGTAACAGGACCAGGCGTAAGTCAAACCACTACCCCTTTTGGCACCGTGTTTGTTTTCCAGGATCCGATGACTGTCACAGCCAATCGATCTTCGAAAGCAGTTGGGATAGCTCAAGGAACTTCTGTCACATCCAGCTTAGATGGGCTTCAGAGCATTTCAGTAGCGAAGATCACTTTGCGCTTGAAGAACCACTCCGGTTCAGTTTCTATAGTCGGAGGCACGCATAATGTGAAGCCCGCCGATCATCCTGTTGTGGGAGGCACCGGCGATTTCCTGTTTGTTCAAGGCTACGTGACATCGTCCCCTGTCGATCTCAGGGGCCTAACTGTTGTCTACAAGATTGCATTTCACCTTTACTGGCCTCCGTATGCAACTCAAGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

191

Amino Acids

20.41

Weight (kDa)

9.69

Isoelectric Point (pI)

22.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 43 - 176 3e-28 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014634)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10500
malus_domestica MD02G1118100.v1.1 MD02G1118400.v1.1
prunus_persica Prupe.7G181300_v2.0.a1
pyrus_communis pycom02g09170 pycom02g09180 pycom15g20710
rosa_chinensis RchiOBHm_Chr2g0097361
rosa_laevigata RLG00000016720
rosa_multiflora Rmu_sc0001060.1_g000010
rosa_roxburghii Rroxscaffold_2G00144740
rosa_rugosa Rorug02G0066000
rosa_samantha Rh2AG112800 Rh2BG115600 Rh2CG117800 Rh2DG116800
rosa_wichuraiana Rw2G008830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 227, 446
AccI GTMKAC 1 cut(s) 519
AciI CCGC 4 cut(s) 51, 72, 100, 423
AclWI GGATC 2 cut(s) 242, 255
AcuI CTGAAG 1 cut(s) 317
AfiI CCNNNNNNNGG 1 cut(s) 440
AgsI TTSAA 3 cut(s) 143, 370, 470
AjnI CCWGG 2 cut(s) 196, 243
AluBI AGCT 3 cut(s) 296, 321, 566
AluI AGCT 3 cut(s) 296, 321, 566
AlwI GGATC 2 cut(s) 242, 255
AoxI GGCC 2 cut(s) 505, 545
AspLEI GCGC 1 cut(s) 366
AspS9I GGNCC 2 cut(s) 194, 505
AsuHPI GGTGA 2 cut(s) 80, 527
AsuII TTCGAA 1 cut(s) 278
AvaII GGWCC 1 cut(s) 194
BamHI GGATCC 1 cut(s) 247
BanI GGYRCC 2 cut(s) 227, 446
BccI CCATC 2 cut(s) 117, 320
BciT130I CCWGG 2 cut(s) 198, 245
BfmI CTRYAG 1 cut(s) 393
BisI GCNGC 1 cut(s) 72
BlsI GCNGC 1 cut(s) 73
Bme1390I CCNGG 2 cut(s) 198, 245
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 2 cut(s) 194, 505
BmiI GGNNCC 4 cut(s) 229, 249, 448, 506
BmrFI CCNGG 2 cut(s) 198, 245
Bpu14I TTCGAA 1 cut(s) 278
BpuEI CTTGAG 3 cut(s) 101, 282, 546
Bsa29I ATCGAT 1 cut(s) 271
BsaAI YACGTR 1 cut(s) 478
BsaWI WCCGGW 3 cut(s) 37, 164, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse118I RCCGGY 1 cut(s) 449
Bse1I ACTGG 1 cut(s) 548
BseBI CCWGG 2 cut(s) 198, 245
BseCI ATCGAT 1 cut(s) 271
BseGI GGATG 2 cut(s) 314, 430
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMII CTCAG 1 cut(s) 514
BseNI ACTGG 1 cut(s) 548
BseRI GAGGAG 1 cut(s) 47
BshFI GGCC 2 cut(s) 507, 547
BshNI GGYRCC 2 cut(s) 227, 446
BshVI ATCGAT 1 cut(s) 271
BsiSI CCGG 4 cut(s) 38, 165, 381, 450
BslFI GGGAC 2 cut(s) 106, 472
BslI CCNNNNNNNGG 1 cut(s) 440
BsmFI GGGAC 2 cut(s) 106, 472
BsnI GGCC 2 cut(s) 507, 547
Bsp119I TTCGAA 1 cut(s) 278
Bsp143I GATC 5 cut(s) 247, 272, 354, 427, 496
BspACI CCGC 4 cut(s) 51, 72, 100, 423
BspANI GGCC 2 cut(s) 507, 547
BspCNI CTCAG 1 cut(s) 513
BspDI ATCGAT 1 cut(s) 271
BspLI GGNNCC 4 cut(s) 229, 249, 448, 506
BspPI GGATC 2 cut(s) 242, 255
BspT104I TTCGAA 1 cut(s) 278
BspT107I GGYRCC 2 cut(s) 227, 446
BsrFI RCCGGY 1 cut(s) 449
BsrI ACTGG 1 cut(s) 548
BssAI RCCGGY 1 cut(s) 449
BssMI GATC 5 cut(s) 247, 272, 354, 427, 496
Bst2UI CCWGG 2 cut(s) 198, 245
Bst4CI ACNGT 3 cut(s) 232, 259, 514
BstBAI YACGTR 1 cut(s) 478
BstBI TTCGAA 1 cut(s) 278
BstC8I GCNNGC 2 cut(s) 407, 423
BstDEI CTNAG 2 cut(s) 322, 500
BstF5I GGATG 2 cut(s) 314, 430
BstHHI GCGC 1 cut(s) 366
BstKTI GATC 5 cut(s) 250, 275, 357, 430, 499
BstMBI GATC 5 cut(s) 247, 272, 354, 427, 496
BstMWI GCNNNNNNNGC 1 cut(s) 563
BstNI CCWGG 2 cut(s) 198, 245
BstSCI CCNGG 2 cut(s) 196, 243
BstSFI CTRYAG 1 cut(s) 393
BstX2I RGATCY 1 cut(s) 247
BstYI RGATCY 1 cut(s) 247
Bsu15I ATCGAT 1 cut(s) 271
BsuRI GGCC 2 cut(s) 507, 547
BsuTUI ATCGAT 1 cut(s) 271
BtsCI GGATG 2 cut(s) 314, 430
Cac8I GCNNGC 2 cut(s) 407, 423
CfoI GCGC 1 cut(s) 366
Cfr10I RCCGGY 1 cut(s) 449
Cfr13I GGNCC 2 cut(s) 194, 505
ClaI ATCGAT 1 cut(s) 271
CviAII CATG 1 cut(s) 95
DdeI CTNAG 2 cut(s) 322, 500
DpnI GATC 5 cut(s) 249, 274, 356, 429, 498
DpnII GATC 5 cut(s) 247, 272, 354, 427, 496
EciI GGCGGA 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 505
EcoRII CCWGG 2 cut(s) 196, 243
FaeI CATG 1 cut(s) 98
FaiI YATR 7 cut(s) 26, 96, 155, 171, 395, 411, 555
FaqI GGGAC 2 cut(s) 106, 472
FatI CATG 1 cut(s) 94
FauI CCCGC 1 cut(s) 430
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 1 cut(s) 72
FokI GGATG 2 cut(s) 301, 417
Fsp4HI GCNGC 1 cut(s) 72
GlaI GCGC 1 cut(s) 365
GluI GCNGC 1 cut(s) 72
HaeIII GGCC 2 cut(s) 507, 547
HapII CCGG 4 cut(s) 38, 165, 381, 450
HhaI GCGC 1 cut(s) 366
Hin1II CATG 1 cut(s) 98
Hin6I GCGC 1 cut(s) 364
HinP1I GCGC 1 cut(s) 364
HindIII AAGCTT 1 cut(s) 564
HinfI GANTC 1 cut(s) 18
HpaII CCGG 4 cut(s) 38, 165, 381, 450
HphI GGTGA 2 cut(s) 80, 527
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 3 cut(s) 252, 336, 401
Hpy8I GTNNAC 1 cut(s) 520
HpyCH4III ACNGT 3 cut(s) 232, 259, 514
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 2 cut(s) 530, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 563
HpyF3I CTNAG 2 cut(s) 322, 500
HpySE526I ACGT 1 cut(s) 477
Hsp92II CATG 1 cut(s) 98
HspAI GCGC 1 cut(s) 364
Kzo9I GATC 5 cut(s) 247, 272, 354, 427, 496
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 4 cut(s) 187, 259, 310, 478
MalI GATC 5 cut(s) 249, 274, 356, 429, 498
MboI GATC 5 cut(s) 247, 272, 354, 427, 496
MboII GAAGA 5 cut(s) 23, 26, 267, 364, 382
MflI RGATCY 1 cut(s) 247
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 7 cut(s) 65, 68, 85, 134, 395, 437, 558
MseI TTAA 1 cut(s) 571
MslI CAYNNNNRTG 1 cut(s) 93
MspI CCGG 4 cut(s) 38, 165, 381, 450
MspR9I CCNGG 2 cut(s) 198, 245
MvaI CCWGG 2 cut(s) 198, 245
MwoI GCNNNNNNNGC 1 cut(s) 563
NdeII GATC 5 cut(s) 247, 272, 354, 427, 496
NlaIII CATG 1 cut(s) 98
NlaIV GGNNCC 4 cut(s) 229, 249, 448, 506
NmuCI GTSAC 3 cut(s) 259, 310, 478
NspV TTCGAA 1 cut(s) 278
PfeI GAWTC 1 cut(s) 18
PflFI GACNNNGTC 1 cut(s) 484
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 1 cut(s) 73
Ppu21I YACGTR 1 cut(s) 478
Psp6I CCWGG 2 cut(s) 196, 243
PspGI CCWGG 2 cut(s) 196, 243
PspN4I GGNNCC 4 cut(s) 229, 249, 448, 506
PspPI GGNCC 2 cut(s) 194, 505
PsuI RGATCY 1 cut(s) 247
PsyI GACNNNGTC 1 cut(s) 484
RseI CAYNNNNRTG 1 cut(s) 93
SaqAI TTAA 1 cut(s) 571
SatI GCNGC 1 cut(s) 72
Sau3AI GATC 5 cut(s) 247, 272, 354, 427, 496
Sau96I GGNCC 2 cut(s) 194, 505
ScrFI CCNGG 2 cut(s) 198, 245
SetI ASST 5 cut(s) 298, 323, 480, 540, 568
SfcI CTRYAG 1 cut(s) 393
SfuI TTCGAA 1 cut(s) 278
SgrAI CRCCGGYG 1 cut(s) 449
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 93
SmlI CTYRAG 3 cut(s) 116, 297, 561
SmoI CTYRAG 3 cut(s) 116, 297, 561
SsiI CCGC 4 cut(s) 51, 72, 100, 423
StyD4I CCNGG 2 cut(s) 196, 243
TaaI ACNGT 3 cut(s) 232, 259, 514
TaiI ACGT 1 cut(s) 480
TaqI TCGA 4 cut(s) 21, 271, 278, 495
TauI GCSGC 1 cut(s) 74
TfiI GAWTC 1 cut(s) 18
Tru1I TTAA 1 cut(s) 571
Tru9I TTAA 1 cut(s) 571
TseFI GTSAC 3 cut(s) 259, 310, 478
Tsp45I GTSAC 3 cut(s) 259, 310, 478
TspGWI ACGGA 1 cut(s) 540
Tth111I GACNNNGTC 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 194
XmiI GTMKAC 1 cut(s) 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.