FvH4_1g13250

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008672
Physical Location & Seq
Forward (+)
7275158 .. 7283290
8133 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g13250.t1

Sequence Viewer

Length: 3150 bp
ATGGAAACCGATAACTCAGTGGACCTGAAGGTGGAGGAGCTCTTGAAAGAGGTCCAGCTTGAATATTCACATGCGCTCACCAAGTTCGTAGACGACGCCGTTTCGTCAATCAAAGACGCCATCGGTCAAATTCCAGAAGACCTCAAGGTTACGGCGGATTTAGCTCCGGGATTCGTCAGAGACATTGGTGCAGACAAAGCTGAATTCGAGTTCAAGAAGCCGAAATCTCTGAAGATTGGCGGCAGTTATGCTATAGGGTTCCTTGTGAAGCCAGAGTTCAATGTGGATCTTTTCGTTCAGTTGCCCAAGGAGTGTTTTCATGAGAAAGACTATTTGAATTACCGGTACCATGCTAAAAGGTGCCTGTACCTTTGTGTGATTAAGAAGTACTTGACGTCGTCAGCCTTAGTTGGTAAGGTAGAGTGGTCCACCTTCCAGAATGAAGTTCGGAAGCCCGTGCTGATTGTGTATCCAGTCAAGAAACTTGTTGCACTCCCTGGGTTTTTTGTAAGAATAATACCTACAGCACCATCCCTTTTTAGCATTCCGAAATTGAACTTGCAGCGGAATAATGTTCGTGCTGTGAGTAAAGGGGGCATTCCTCAGGCTACTCCCAAGTACAATAGTAGCATTTTGGAAGACATGTTTATAGAAGATACCGAAGAAATTGTCAAGCAAACTTTTCTAGGATCCAAGGAATTACGAGAGGGATTAATTTTGCTGAAGGTCTGGGCTCGCCGTAGGACGCCAATATATGCTCATGATTGCCTGAATGGGTTTCTAATCTCTGTCATATTGGCATATCTTGTGGATAGGAACCATGTCAATAAATCAATGAAGGCAATGCAAATATTTCGTGTTACAATGAAATTCATTGCTACTTCAGATTTGTGGAAACATGGCCTCTACTTCATACCAAAGGGCCAAAAAGCCATTTCGAAAGAGGAGAGGCTGCCTTTTAAAGAATCATTTCCGATTGTTATATGTACTCCATCTAGGACCTTTAATTTAGCCTTCCGGATAACAAGGGTTGGATTTCTTGAGCTCCAAAATGAATCTACAATGACACTGGCATGTATAGAGAAATGCAGAGATAGTGGATTTGAGGAAGTTTTTATGACCAAGATTGACTATCCTGTTAAATATGACCATGTGATAAGGTTGAACTTAAAGGGGAAGAGTTCGGTTTATGTATCGGGATTTTGCTTGGATGATGAATGTTGGAGGGTGTACGAGCAGAAGGTTTATAATGTATTAAGTCATGGGTTGAGTGACCGAGTGAAGACTGTACATGTGACCTGGAAAAGCATGTTATCAGAATCCGCTCTACAAAATGGATTGTCGACACTCAATGCGGAGCCATTGCTAATTGGAATTTCAGTAACCTCTTTAGATAAAGCTTTCAGAATTGTTGATATTGGTCCTGATGCTGATAATAAGGAGGAGGCTCTCAAATTCCGCCAGTTCTGGGGGGATAAGGCTGAGCTTAGGAGATTCAAAGATGGAAAAATAGCGGAAAGCACAGTTTGGGAAACTGAGCAATGGAAAAGACACATCGTTATAAAAAAGATTTCTGAGCATGTGCTTCTCCGACATCTTTCACTATCGAAAGAGAATATCTTGCATATCGTGGATCAACTAGATTTCTCTTTGCTATATGGTGCTGAAGATCCTATATCTTCTACCGCGAGTTTGATTGGGGCATTTGAAATTCTATCGAAGCGCTTGCGCCTTATTGAAGACATACCTCTGAAAGTTTCTACGGTGCAGGCCCTAGATTCGGCTTTCAGGTTTTCATCTGTCTTCCCACCTGAACCTCATCCGCTAGCTAATGAAAAGGGTTCTTTCGTAAAACTAAATAAGTTCCCTCCTTCATGCATCCGGCCGCTGGAAGTTATGATTCAGTTGGAAGGTTCTGGAAACTGGCCTATGGATGATGTAGCAATTGAGAAAACCAAATCAGCATTCCTTCTTAAAATCGGAGAGAGTCTTCAGAATAGTTGGGGGATGACATGTACAGCCACGGAGGATGATGTGGATGTTTTTGTCTCTGGATATGCCTTTCGCCTTAAAATTTGGCATGAGAGAGGCCTAACTTTGATGAGACGAGAAACTGGGAATGAGCATGTAAATAAGGTTTCTAATGTGGACAAGGAACTATACTTCCGCAGTCAACATTCCAGCATGATCAATGGATTACAGACTTGTTACCCAGCATATGGGCCAGTTGTTAGGCTTGCAAAACGATGGGCTGCTTCACATCTATTTTCGGCTTGCTTGGAAGAGGAGGCAGTTGAGCTATTGGTGGCATATATATTTTTGAAGCCTTTACCATTTAATGCTCCATGCTCACGGATCACTGGATTTTTAAGGTTCTTGCGTTTACTATCAGACTATGACTGGACCTTCTCTGCATTAGTTGTTGACATAAATAATGATTTGACGCCAAATGATGAGAAAGAGATAAGAGAAAACTTTATGTTTAGTAGAAAAGGCTATGAAGAAAATCCTCAAAATGTAAACTCAGCACTGTTCTTAGCTACAGCATACGACAAGGCATCTGAGGCCTGGACCAGATTTTCACCAAATTCAGTGGAGCTGAAAAGGTTGGTGGCCTATGCTGGAAGCAGTGCAAACTTGTTGACCAAACTCATATTGGAGGACCAGAGTGATTCTTATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAATTACGACGCTGTGATTCTTCTTCATAGAGAAAAATTACCTTATCCCCAACGCCTTCTCTTTCCATCCGAATTGCATCAAGGGGTGCATGTGGCACGTGGGAATGCAAGCAAGAGTTTCCACCCCTTTCTTTTGCCTGGAGATTTTAAAGGAAGCTTAGAGGATCTAAGAAACAAAGTATTGGTAAACTTTGATCCGCTGAGGTGTTTTATTGGGGATCTAGAGGCAAAGTACTCCAACGCTTTCAAGCTATGGTATGATTCTCTCGGTGGTGATGCTGTTGGCATAACATGGGGAGGATGCAGTTCAAAGAAACGGGGGCGGGAAGAAGCAGATGAGGAAGTAAAAGATCCAATTGATTTGTTAAAAGATGTTGGTAAAGTCGGAACAGGGTTCGTGAGGGGCATTTATCTTCTCAAAGCCCCAAGGCGGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

1050

Amino Acids

119.39

Weight (kDa)

8.39

Isoelectric Point (pI)

38.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 95 - 227 8.1e-35 Nrap protein domain 1
Nrap_D2 PF17403 234 - 375 1.3e-31 Nrap protein PAP/OAS-like domain
Nrap_D3 PF17404 380 - 534 1.1e-38 Nrap protein domain 3
Nrap_D4 PF17405 556 - 735 9e-45 Nrap protein nucleotidyltransferase domain 4
Nrap_D5 PF17406 737 - 889 2.1e-46 Nrap protein PAP/OAS1-like domain 5
Nrap_D6 PF17407 903 - 1010 5.4e-12 Nrap protein domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1248, 1562
AatII GACGTC 1 cut(s) 398
Acc65I GGTACC 1 cut(s) 345
AccB1I GGYRCC 2 cut(s) 345, 360
AccB7I CCANNNNNTGG 1 cut(s) 2219
AccBSI CCGCTC 1 cut(s) 1325
AccI GTMKAC 2 cut(s) 90, 1343
AccII CGCG 1 cut(s) 1688
AccIII TCCGGA 1 cut(s) 1017
AcoI YGGCCR 1 cut(s) 1883
AcsI RAATTY 8 cut(s) 129, 203, 869, 1374, 1454, 1710, 2073, 2587
AcuI CTGAAG 6 cut(s) 47, 251, 743, 867, 1686, 1976
AcvI CACGTG 1 cut(s) 2810
AcyI GRCGYC 5 cut(s) 96, 117, 395, 746, 2444
AfaI GTAC 9 cut(s) 347, 368, 389, 620, 988, 1232, 1290, 2017, 2945
AfeI AGCGCT 1 cut(s) 1724
AfiI CCNNNNNNNGG 6 cut(s) 31, 1468, 1780, 1888, 2219, 3141
AflIII ACRYGT 3 cut(s) 642, 1291, 2012
AgeI ACCGGT 1 cut(s) 342
AjnI CCWGG 4 cut(s) 496, 1298, 2567, 2848
AjuI GAANNNNNNNTTGG 2 cut(s) 1949, 1981
AloI GAACNNNNNNTCC 2 cut(s) 2148, 2180
Alw21I GWGCWC 2 cut(s) 42, 1047
Alw26I GTCTC 3 cut(s) 174, 2053, 2098
Aor13HI TCCGGA 1 cut(s) 1017
Aor51HI AGCGCT 1 cut(s) 1724
AoxI GGCC 9 cut(s) 901, 922, 1770, 1883, 1925, 2089, 2222, 2565, 2613
ApeKI GCWGC 3 cut(s) 562, 952, 2252
ApoI RAATTY 8 cut(s) 129, 203, 869, 1374, 1454, 1710, 2073, 2587
ArsI GACNNNNNNTTYG 2 cut(s) 654, 686
AseI ATTAAT 1 cut(s) 713
AsiGI ACCGGT 1 cut(s) 342
Asp700I GAANNNNTTC 1 cut(s) 969
Asp718I GGTACC 1 cut(s) 345
AspLEI GCGC 3 cut(s) 76, 1725, 1731
AsuC2I CCSGG 1 cut(s) 168
AsuHPI GGTGA 3 cut(s) 70, 2574, 2996
AsuII TTCGAA 2 cut(s) 938, 2698
AsuNHI GCTAGC 1 cut(s) 1825
AvaII GGWCC 8 cut(s) 22, 52, 426, 999, 1421, 2403, 2571, 2662
AxyI CCTNAGG 1 cut(s) 603
BamHI GGATCC 1 cut(s) 689
BanI GGYRCC 2 cut(s) 345, 360
BanII GRGCYC 3 cut(s) 42, 736, 1047
BbrPI CACGTG 1 cut(s) 2810
BbsI GAAGAC 6 cut(s) 144, 645, 1289, 1746, 1795, 1982
Bbv12I GWGCWC 2 cut(s) 42, 1047
BbvCI CCTCAGC 1 cut(s) 2912
BbvI GCAGC 3 cut(s) 574, 939, 2239
BccI CCATC 7 cut(s) 128, 538, 1000, 1496, 2241, 2676, 2785
BceAI ACGGC 3 cut(s) 83, 168, 723
BcgI CGANNNNNNTGC 4 cut(s) 836, 870, 1708, 1742
BciT130I CCWGG 4 cut(s) 498, 1300, 2569, 2850
BciVI GTATCC 1 cut(s) 480
BclI TGATCA 1 cut(s) 2187
BcnI CCSGG 1 cut(s) 168
BcoDI GTCTC 3 cut(s) 174, 2053, 2098
BfaI CTAG 6 cut(s) 686, 996, 1640, 1775, 1826, 2933
BfmI CTRYAG 3 cut(s) 252, 522, 2541
BfoI RGCGCY 1 cut(s) 1726
BfuI GTATCC 1 cut(s) 480
BisI GCNGC 5 cut(s) 241, 563, 953, 1886, 2253
BlpI GCTNAGC 1 cut(s) 1482
BlsI GCNGC 5 cut(s) 242, 564, 954, 1887, 2254
BmcAI AGTACT 2 cut(s) 389, 2945
Bme1390I CCNGG 5 cut(s) 168, 498, 1300, 2569, 2850
Bme18I GGWCC 8 cut(s) 22, 52, 426, 999, 1421, 2403, 2571, 2662
BmiI GGNNCC 6 cut(s) 260, 347, 362, 691, 818, 1359
BmrFI CCNGG 5 cut(s) 168, 498, 1300, 2569, 2850
BmrI ACTGGG 1 cut(s) 2124
BmsI GCATC 6 cut(s) 1417, 1887, 2567, 2797, 2977, 3002
BmtI GCTAGC 1 cut(s) 1829
BmuI ACTGGG 1 cut(s) 2124
BpiI GAAGAC 6 cut(s) 144, 645, 1289, 1746, 1795, 1982
BpmI CTGGAG 1 cut(s) 2871
Bpu10I CCTNAGC 2 cut(s) 1487, 2912
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 2 cut(s) 938, 2698
BpuEI CTTGAG 2 cut(s) 128, 1061
BpuMI CCSGG 1 cut(s) 168
BsaAI YACGTR 1 cut(s) 2810
BsaHI GRCGYC 5 cut(s) 96, 117, 395, 746, 2444
BsaJI CCNNGG 6 cut(s) 306, 496, 497, 693, 2022, 3137
BsaWI WCCGGW 2 cut(s) 342, 1017
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 1468, 1780, 1888, 2219, 3141
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 8 cut(s) 473, 1074, 1462, 1928, 2119, 2225, 2365, 2405
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 4 cut(s) 849, 873, 1361, 1547
BseAI TCCGGA 1 cut(s) 1017
BseBI CCWGG 4 cut(s) 498, 1300, 2569, 2850
BseDI CCNNGG 6 cut(s) 306, 496, 497, 693, 2022, 3137
BseLI CCNNNNNNNGG 6 cut(s) 31, 1468, 1780, 1888, 2219, 3141
BseMI GCAATG 4 cut(s) 849, 873, 1361, 1547
BseMII CTCAG 8 cut(s) 30, 617, 1473, 1527, 1566, 2538, 2553, 2903
BseNI ACTGG 8 cut(s) 473, 1074, 1462, 1928, 2119, 2225, 2365, 2405
BseRI GAGGAG 4 cut(s) 50, 959, 1457, 2300
BseX3I CGGCCG 1 cut(s) 1883
BseXI GCAGC 3 cut(s) 574, 939, 2239
BseYI CCCAGC 1 cut(s) 2212
BsgI GTGCAG 2 cut(s) 210, 1787
Bsh1236I CGCG 1 cut(s) 1688
Bsh1285I CGRYCG 1 cut(s) 1886
BshFI GGCC 9 cut(s) 903, 924, 1772, 1885, 1927, 2091, 2224, 2567, 2615
BshNI GGYRCC 2 cut(s) 345, 360
BshTI ACCGGT 1 cut(s) 342
BsiEI CGRYCG 1 cut(s) 1886
BsiHKAI GWGCWC 2 cut(s) 42, 1047
BsiSI CCGG 4 cut(s) 167, 343, 1018, 1882
BslI CCNNNNNNNGG 6 cut(s) 31, 1468, 1780, 1888, 2219, 3141
BsmAI GTCTC 3 cut(s) 174, 2053, 2098
BsmBI CGTCTC 1 cut(s) 2098
BsmI GAATGC 4 cut(s) 543, 597, 1964, 2821
BsnI GGCC 9 cut(s) 903, 924, 1772, 1885, 1927, 2091, 2224, 2567, 2615
Bsp119I TTCGAA 2 cut(s) 938, 2698
Bsp1286I GDGCHC 3 cut(s) 42, 736, 1047
Bsp13I TCCGGA 1 cut(s) 1017
Bsp1407I TGTACA 2 cut(s) 1288, 2015
Bsp1720I GCTNAGC 1 cut(s) 1482
BspANI GGCC 9 cut(s) 903, 924, 1772, 1885, 1927, 2091, 2224, 2567, 2615
BspCNI CTCAG 8 cut(s) 29, 616, 1474, 1528, 1567, 2537, 2554, 2904
BspEI TCCGGA 1 cut(s) 1017
BspFNI CGCG 1 cut(s) 1688
BspHI TCATGA 2 cut(s) 319, 760
BspLI GGNNCC 6 cut(s) 260, 347, 362, 691, 818, 1359
BspOI GCTAGC 1 cut(s) 1829
BspT104I TTCGAA 2 cut(s) 938, 2698
BspT107I GGYRCC 2 cut(s) 345, 360
BsrBI CCGCTC 1 cut(s) 1325
BsrDI GCAATG 4 cut(s) 849, 873, 1361, 1547
BsrFI RCCGGY 1 cut(s) 342
BsrGI TGTACA 2 cut(s) 1288, 2015
BsrI ACTGG 8 cut(s) 473, 1074, 1462, 1928, 2119, 2225, 2365, 2405
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 6 cut(s) 306, 496, 497, 693, 2022, 3137
BssNI GRCGYC 5 cut(s) 96, 117, 395, 746, 2444
BssT1I CCWWGG 3 cut(s) 306, 693, 3137
Bst2UI CCWGG 4 cut(s) 498, 1300, 2569, 2850
Bst4CI ACNGT 4 cut(s) 1288, 1525, 1765, 2532
Bst6I CTCTTC 2 cut(s) 1172, 2277
BstACI GRCGYC 5 cut(s) 96, 117, 395, 746, 2444
BstAUI TGTACA 2 cut(s) 1288, 2015
BstBAI YACGTR 1 cut(s) 2810
BstBI TTCGAA 2 cut(s) 938, 2698
BstC8I GCNNGC 7 cut(s) 736, 1727, 1770, 1827, 2238, 2275, 2821
BstDSI CCRYGG 1 cut(s) 2022
BstFNI CGCG 1 cut(s) 1688
BstH2I RGCGCY 1 cut(s) 1726
BstHHI GCGC 3 cut(s) 76, 1725, 1731
BstMAI GTCTC 3 cut(s) 174, 2053, 2098
BstMCI CGRYCG 1 cut(s) 1886
BstMWI GCNNNNNNNGC 3 cut(s) 161, 197, 2564
BstNI CCWGG 4 cut(s) 498, 1300, 2569, 2850
BstNSI RCATGY 9 cut(s) 74, 646, 1077, 1295, 1312, 1583, 2016, 2129, 2804
BstSCI CCNGG 5 cut(s) 166, 496, 1298, 2567, 2848
BstSFI CTRYAG 3 cut(s) 252, 522, 2541
BstUI CGCG 1 cut(s) 1688
BstV1I GCAGC 3 cut(s) 574, 939, 2239
BstV2I GAAGAC 6 cut(s) 144, 645, 1289, 1746, 1795, 1982
BstX2I RGATCY 6 cut(s) 286, 689, 1669, 2875, 2929, 3061
BstYI RGATCY 6 cut(s) 286, 689, 1669, 2875, 2929, 3061
BstZI CGGCCG 1 cut(s) 1883
Bsu36I CCTNAGG 1 cut(s) 603
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 9 cut(s) 903, 924, 1772, 1885, 1927, 2091, 2224, 2567, 2615
BtgI CCRYGG 1 cut(s) 2022
BtsI GCAGTG 1 cut(s) 2635
BtsIMutI CAGTG 6 cut(s) 24, 1067, 2358, 2528, 2598, 2635
Cac8I GCNNGC 7 cut(s) 736, 1727, 1770, 1827, 2238, 2275, 2821
CciI TCATGA 2 cut(s) 319, 760
CfoI GCGC 3 cut(s) 76, 1725, 1731
Cfr10I RCCGGY 1 cut(s) 342
CseI GACGC 5 cut(s) 104, 125, 754, 2452, 2729
Csp6I GTAC 9 cut(s) 346, 367, 388, 619, 987, 1231, 1289, 2016, 2944
CspAI ACCGGT 1 cut(s) 342
CspCI CAANNNNNGTGG 2 cut(s) 2574, 2609
CviQI GTAC 9 cut(s) 346, 367, 388, 619, 987, 1231, 1289, 2016, 2944
DraI TTTAAA 2 cut(s) 961, 2860
EaeI YGGCCR 1 cut(s) 1883
EagI CGGCCG 1 cut(s) 1883
Eam1104I CTCTTC 2 cut(s) 1172, 2277
EarI CTCTTC 2 cut(s) 1172, 2277
EciI GGCGGA 2 cut(s) 170, 1448
Ecl136II GAGCTC 2 cut(s) 40, 1045
EclXI CGGCCG 1 cut(s) 1883
Eco130I CCWWGG 3 cut(s) 306, 693, 3137
Eco147I AGGCCT 2 cut(s) 2091, 2567
Eco24I GRGCYC 3 cut(s) 42, 736, 1047
Eco47I GGWCC 8 cut(s) 22, 52, 426, 999, 1421, 2403, 2571, 2662
Eco47III AGCGCT 1 cut(s) 1724
Eco52I CGGCCG 1 cut(s) 1883
Eco53kI GAGCTC 2 cut(s) 40, 1045
Eco57I CTGAAG 6 cut(s) 47, 251, 743, 867, 1686, 1976
Eco72I CACGTG 1 cut(s) 2810
Eco81I CCTNAGG 1 cut(s) 603
EcoICRI GAGCTC 2 cut(s) 40, 1045
EcoO109I RGGNCCY 2 cut(s) 999, 1771
EcoRI GAATTC 1 cut(s) 203
EcoRII CCWGG 4 cut(s) 496, 1298, 2567, 2848
EcoT14I CCWWGG 3 cut(s) 306, 693, 3137
EcoT22I ATGCAT 1 cut(s) 1880
EcoT38I GRGCYC 3 cut(s) 42, 736, 1047
ErhI CCWWGG 3 cut(s) 306, 693, 3137
Esp3I CGTCTC 1 cut(s) 2098
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FauI CCCGC 1 cut(s) 3027
FauNDI CATATG 1 cut(s) 2218
FbaI TGATCA 1 cut(s) 2187
FblI GTMKAC 2 cut(s) 90, 1343
Fnu4HI GCNGC 5 cut(s) 241, 563, 953, 1886, 2253
FriOI GRGCYC 3 cut(s) 42, 736, 1047
Fsp4HI GCNGC 5 cut(s) 241, 563, 953, 1886, 2253
FspBI CTAG 6 cut(s) 686, 996, 1640, 1775, 1826, 2933
GlaI GCGC 3 cut(s) 75, 1724, 1730
GluI GCNGC 5 cut(s) 241, 563, 953, 1886, 2253
GsaI CCCAGC 1 cut(s) 2216
GsuI CTGGAG 1 cut(s) 2871
HaeII RGCGCY 1 cut(s) 1726
HaeIII GGCC 9 cut(s) 903, 924, 1772, 1885, 1927, 2091, 2224, 2567, 2615
HapII CCGG 4 cut(s) 167, 343, 1018, 1882
HgaI GACGC 5 cut(s) 104, 125, 754, 2452, 2729
HhaI GCGC 3 cut(s) 76, 1725, 1731
Hin1I GRCGYC 5 cut(s) 96, 117, 395, 746, 2444
Hin6I GCGC 3 cut(s) 74, 1723, 1729
HinP1I GCGC 3 cut(s) 74, 1723, 1729
HincII GTYRAC 4 cut(s) 1344, 2174, 2425, 2643
HindII GTYRAC 4 cut(s) 1344, 2174, 2425, 2643
HindIII AAGCTT 2 cut(s) 1398, 2866
HpaII CCGG 4 cut(s) 167, 343, 1018, 1882
HphI GGTGA 3 cut(s) 70, 2574, 2996
Hpy99I CGWCG 3 cut(s) 98, 400, 2723
HpyCH4III ACNGT 4 cut(s) 1288, 1525, 1765, 2532
HpyCH4IV ACGT 2 cut(s) 395, 2809
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 197, 2564
HpySE526I ACGT 2 cut(s) 395, 2809
Hsp92I GRCGYC 5 cut(s) 96, 117, 395, 746, 2444
HspAI GCGC 3 cut(s) 74, 1723, 1729
Kpn2I TCCGGA 1 cut(s) 1017
KpnI GGTACC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 2187
LmnI GCTCC 6 cut(s) 37, 169, 1050, 1357, 2347, 2596
Lsp1109I GCAGC 3 cut(s) 574, 939, 2239
LweI GCATC 6 cut(s) 1417, 1887, 2567, 2797, 2977, 3002
MaeI CTAG 6 cut(s) 686, 996, 1640, 1775, 1826, 2933
MaeII ACGT 2 cut(s) 395, 2809
MaeIII GTNAC 6 cut(s) 148, 859, 1271, 1294, 1381, 2207
MbiI CCGCTC 1 cut(s) 1325
MfeI CAATTG 2 cut(s) 1944, 3066
MflI RGATCY 6 cut(s) 286, 689, 1669, 2875, 2929, 3061
MhlI GDGCHC 3 cut(s) 42, 736, 1047
MlyI GAGTC 1 cut(s) 1996
MmeI TCCRAC 6 cut(s) 1012, 1202, 1615, 1887, 2973, 3076
Mph1103I ATGCAT 1 cut(s) 1880
MroI TCCGGA 1 cut(s) 1017
MroXI GAANNNNTTC 1 cut(s) 969
MslI CAYNNNNRTG 1 cut(s) 1072
MspA1I CMGCKG 3 cut(s) 565, 1888, 2911
MspI CCGG 4 cut(s) 167, 343, 1018, 1882
MspR9I CCNGG 5 cut(s) 168, 498, 1300, 2569, 2850
MunI CAATTG 2 cut(s) 1944, 3066
Mva1269I GAATGC 4 cut(s) 543, 597, 1964, 2821
MvaI CCWGG 4 cut(s) 498, 1300, 2569, 2850
MvnI CGCG 1 cut(s) 1688
MwoI GCNNNNNNNGC 3 cut(s) 161, 197, 2564
NciI CCSGG 1 cut(s) 168
NdeI CATATG 1 cut(s) 2218
NheI GCTAGC 1 cut(s) 1825
NlaIV GGNNCC 6 cut(s) 260, 347, 362, 691, 818, 1359
NmuCI GTSAC 2 cut(s) 1271, 1294
NsiI ATGCAT 1 cut(s) 1880
NspI RCATGY 9 cut(s) 74, 646, 1077, 1295, 1312, 1583, 2016, 2129, 2804
NspV TTCGAA 2 cut(s) 938, 2698
PagI TCATGA 2 cut(s) 319, 760
PasI CCCWGGG 1 cut(s) 497
PceI AGGCCT 2 cut(s) 2091, 2567
PciI ACATGT 3 cut(s) 642, 1291, 2012
PctI GAATGC 4 cut(s) 543, 597, 1964, 2821
PdmI GAANNNNTTC 1 cut(s) 969
PflFI GACNNNGTC 1 cut(s) 397
PflMI CCANNNNNTGG 1 cut(s) 2219
PfoI TCCNGGA 1 cut(s) 166
PinAI ACCGGT 1 cut(s) 342
PkrI GCNGC 5 cut(s) 242, 564, 954, 1887, 2254
PleI GAGTC 1 cut(s) 1995
PmaCI CACGTG 1 cut(s) 2810
PmlI CACGTG 1 cut(s) 2810
PpsI GAGTC 1 cut(s) 1995
Ppu21I YACGTR 1 cut(s) 2810
PpuMI RGGWCCY 1 cut(s) 999
PscI ACATGT 3 cut(s) 642, 1291, 2012
PshBI ATTAAT 1 cut(s) 713
PsiI TTATAA 2 cut(s) 1248, 1562
Psp124BI GAGCTC 2 cut(s) 42, 1047
Psp5II RGGWCCY 1 cut(s) 999
Psp6I CCWGG 4 cut(s) 496, 1298, 2567, 2848
PspCI CACGTG 1 cut(s) 2810
PspFI CCCAGC 1 cut(s) 2212
PspGI CCWGG 4 cut(s) 496, 1298, 2567, 2848
PspN4I GGNNCC 6 cut(s) 260, 347, 362, 691, 818, 1359
PspPPI RGGWCCY 1 cut(s) 999
PsuI RGATCY 6 cut(s) 286, 689, 1669, 2875, 2929, 3061
PsyI GACNNNGTC 1 cut(s) 397
RsaI GTAC 9 cut(s) 347, 368, 389, 620, 988, 1232, 1290, 2017, 2945
RsaNI GTAC 9 cut(s) 346, 367, 388, 619, 987, 1231, 1289, 2016, 2944
RseI CAYNNNNRTG 1 cut(s) 1072
SacI GAGCTC 2 cut(s) 42, 1047
SalI GTCGAC 1 cut(s) 1342
SatI GCNGC 5 cut(s) 241, 563, 953, 1886, 2253
ScaI AGTACT 2 cut(s) 389, 2945
SchI GAGTC 1 cut(s) 1996
ScrFI CCNGG 5 cut(s) 168, 498, 1300, 2569, 2850
SduI GDGCHC 3 cut(s) 42, 736, 1047
SfaNI GCATC 6 cut(s) 1417, 1887, 2567, 2797, 2977, 3002
SfcI CTRYAG 3 cut(s) 252, 522, 2541
SfuI TTCGAA 2 cut(s) 938, 2698
SinI GGWCC 8 cut(s) 22, 52, 426, 999, 1421, 2403, 2571, 2662
SmiMI CAYNNNNRTG 1 cut(s) 1072
SmlI CTYRAG 2 cut(s) 143, 1040
SmoI CTYRAG 2 cut(s) 143, 1040
SseBI AGGCCT 2 cut(s) 2091, 2567
SspI AATATT 2 cut(s) 65, 852
SspMI CTAG 6 cut(s) 686, 996, 1640, 1775, 1826, 2933
SstI GAGCTC 2 cut(s) 42, 1047
StuI AGGCCT 2 cut(s) 2091, 2567
StyD4I CCNGG 5 cut(s) 166, 496, 1298, 2567, 2848
StyI CCWWGG 3 cut(s) 306, 693, 3137
TaaI ACNGT 4 cut(s) 1288, 1525, 1765, 2532
TaiI ACGT 2 cut(s) 398, 2812
TaqI TCGA 6 cut(s) 207, 938, 1343, 1607, 1718, 2698
TaqII GACCGA 2 cut(s) 113, 1290
TatI WGTACW 6 cut(s) 387, 618, 986, 1288, 2015, 2943
TauI GCSGC 2 cut(s) 243, 1888
TscAI CASTG 6 cut(s) 24, 1074, 2365, 2535, 2598, 2635
TseFI GTSAC 2 cut(s) 1271, 1294
TseI GCWGC 3 cut(s) 562, 952, 2252
Tsp45I GTSAC 2 cut(s) 1271, 1294
TspGWI ACGGA 2 cut(s) 2039, 2368
TspRI CASTG 6 cut(s) 24, 1074, 2365, 2535, 2598, 2635
Tth111I GACNNNGTC 1 cut(s) 397
Van91I CCANNNNNTGG 1 cut(s) 2219
VpaK11BI GGWCC 8 cut(s) 22, 52, 426, 999, 1421, 2403, 2571, 2662
VspI ATTAAT 1 cut(s) 713
XapI RAATTY 8 cut(s) 129, 203, 869, 1374, 1454, 1710, 2073, 2587
XbaI TCTAGA 1 cut(s) 2932
XceI RCATGY 9 cut(s) 74, 646, 1077, 1295, 1312, 1583, 2016, 2129, 2804
XcmI CCANNNNNNNNNTGG 1 cut(s) 2653
XmiI GTMKAC 2 cut(s) 90, 1343
XmnI GAANNNNTTC 1 cut(s) 969
XspI CTAG 6 cut(s) 686, 996, 1640, 1775, 1826, 2933
ZraI GACGTC 1 cut(s) 396
ZrmI AGTACT 2 cut(s) 389, 2945
Zsp2I ATGCAT 1 cut(s) 1880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.