Rroxscaffold_2G00141310

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008672
Physical Location & Seq
Reverse (-)
79603572 .. 79611912
8341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00141310.1

Sequence Viewer

Length: 3138 bp
ATGGAAACCGATAACTCAGCGGACCTGAAGGTGGAGGAGCTCTTAAAAGAGGTCCAGCTAGAATATTCACCTGCGCTCACCAAGTTCGTAGACGACGCCGTTTCCTCGATCAAAGCAGCCATCGATACAATTCCACAAGACCTCAAGGTTACGGCTGATTTAGCTCCGGGATTCGTCAGAGATATTGGTGCAGACAAAGTTGAATTTGATTTCAAGAAGCCGAAATCTGTCAAAATTGGCGGCAGTTATGCTATAGAGTGCCTTGTAAAGCCTGAGTTCAATGTGGATCTTTTCGTTCAGTTGCCCAAGGAATGTTTCCATGAGAAAGACTATTTGAATTACCGGTACCATGCTAAAAGGTGCCTGTACCTTTGTGTGATTAAAAAGTACTTGATGTTGTCTTCGTTAGTTGGTAAGGTTGAATGGTCCACTTTCCAGAACGAAGTCCGGAAACCTGTGTTGATTGTGTATCCAGTCACGAAATTAGTTGCACTCCCTGGGTTCTTTGTGAGAATAATTCCCACAGCTCCATCTCTTTTTAGCATTCCAAAATTGAACCTGCAGCGGAATAATGTTCGCGCTGTGAGTAAAGGGGGCATTCCTCAGGCTACTCCCAGGTACAATAGTAGTATTTTGGAAGACATGTTTATAGAAGATACAGAAGAACTTGTCAAGCAAACTTTTCTTGGGTCTAAGGAATTACGACAAGGTTTAGTTGTGCTGAAGGTCTGGGCTCGCCGCAGGATGCCAATATATGCTCATGATTGCCTGAATGGTTTTCTAATCTCTGTCATATTGGCATACCTTGTGGATAGGAACCATATCAATAAATCAATGAAGGCAATGCAAATATTTCGTGTTACAATGAAATTCATTGCTACTTCAGATTTGTGGAAACACGGGCTCTACTTCACACCAAAAGGCCAAAAAGCTGTTTCAAAAGAGGAGAGGTTGCCTTTTAAAGAATCATTTCCAATTGTTATATGTACTCCATCTAGAAGCTTTAATATAGCCTTCCGGGTAACAAGGGTTGGATTTCTCGAGCTGCAAAATGAATCTACAATGACATTGGCATGCATTGAGAAATGCAGAGATAGTGGATTTGAGGAAGTATTTATGACCAGGATTGACTATCCTGTAAAATATGACCATGTCATAAGGTTGAACTTAAAGGGGAAGAGTTCAGTTTATGGATCGGGATTTTGCTTGGATGATGAATGTTGGAGAGTGTATGAGCAGAAGGTTCATAATGTTTTAAGTCATGGGTTGAGTGACCGGGTGAAGACTGTACGTGTGACCTGGAAAAGCATGTTATCAGAATCCATTCTACAAAATGGATTGTCGTCACTCAATACAGAGCCACTGCTAATTGGAATTTCATTGAGCTCTTTAGATAAAGCTTTAAGAATTGTTGATATTGGTCCTGATGCTGATAATAAGGAGGAGGCTCTCAAATTCCGTCAGTTCTGGGGGGATAAGGCTGAGCTTAGGAGATTCAAAGATGGCAAGATAGCAGAAAGCACAGTTTGGGAAACCGAGCAATGGAAAAGACATGTCGTCATAAAAAAGATATCTGAGCATGTGCTTCTGCGACATCTTTCACTATCGAAAGAGAATATCATGCATATCGTGGATCAACTTGATTTCTCTTTACTATATGGTGCCGAAGATCCTATATCTTCTTCCGGGAGTTTGTTTGGGGCATTTGAAATTCTATCGAAGAGATTGCGCCTTATTGAAGACATTCCTCTAAAGGTTTCTACAGTGCAGGCCCTAGATTCGGCTTTCAGGTATTCATCTGTCTTCCCACCTGAACCTCATCCGCTAGCTAATGAAAAGGGTTCTTTCGTAAGACTAAATCAGTTCCCTCCTTCCTGCATCCGGCCGCTGGAATTGGAAGGTTCTGGAAACTGGCCTATGGATGATGTAGCAATTGAGAAAACTAAATCAGCATTCCTTCTCAAAATCGGAGAGAGTCTTCAGAATAGTTGGGGGATGACATGTACAGCCACTGAGGATGATGTGGATGTTTTTGTCTCTGGATATGCCTTTCGTCTTCAAATTTGGCATGAGAGAGGCCTGACTTTGTTGAGACGAGAAACTGGGAATGAGCATGTGAAGAAGGTTTCTAATGTGGACAAGGAACTGTACTTTCGCAGTCAACATTCCAGCATGATAAATGGATTCCAGACTTGTTACCCAGCATATGGGTCAGTTGTTAGGCTTGCAAAACGATGGGCTGCTTCACATCTATTTTCGGCTTGCTTGGTAGAGGAGGCAATTGAGCTATTGGTAGCATATATATTTTTGAAGCCTTTACCATTTAATGCTCCATGCTCACGGATCACTGGATTTTTAAGGTTCCTGCGATTACTATCTGATTATGACTGGACCTTTTCTGCATTAGTTGTTGACATAAATAATGATTTAACATCAAATGATGAGAAAGAGATAAGGGAGAACTTCATGTTAAGTAGAAAAGGTTATGAAGAAAATCCTCAAAATGTAAACTCAGCAATGTTCCTAGCTACATCCTACGACAAGGCATCTGAGGCTTGGACCAAATTTTCACCAAATTCAATGGAGCTAAAAAGGTTGATGGCTTATGCTGGAAGCAGTGCAAACTTGTTGACCAAACTCATATTGGAGGACCATAGTGATTCTTATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAATTATGATGCTGTAATTCTTCTCCATAGAGAAAAATTACCTTATCCCCAACGCCTTCTCTTTCCATCAGAATTACATCAAGGGGTTCATGTGGCACGTGGGAATGCAAGCAATGGTTTCCACCCCTTTCTTTTGCCTGGAGATTTTAAAGGAAGCTCAGAGGATCTAAGAAACAAAGTATTGGTAAACTTTGATCCGCTAAGGTGTTTTGTTGGGGATCTAGAGGCAAAGTACTCAAACACTCTCAAGCTATGGTATGATTCTCTGGGTGGTGATGCTGTTGGCATAACATGGGGAGGATGTATTTCAAAGAAACGGGGCCGGGAAGAAGCAGATGAGGAAGTAAAGGATCCAATTGATTTGTTAAAAGATGTTGGTAAAGTTGGAACAGGGTTCGTCAGGGGCATTTATCTTCTAAAAGCCCCAAGGCGGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

1045

Amino Acids

118.93

Weight (kDa)

8.39

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 95 - 227 1.8e-34 Nrap protein domain 1
Nrap_D2 PF17403 234 - 375 2.3e-30 Nrap protein PAP/OAS-like domain
Nrap_D3 PF17404 380 - 534 3.6e-38 Nrap protein domain 3
Nrap_D4 PF17405 556 - 731 7e-40 Nrap protein nucleotidyltransferase domain 4
Nrap_D5 PF17406 733 - 885 1.4e-46 Nrap protein PAP/OAS1-like domain 5
Nrap_D6 PF17407 899 - 1006 1.6e-12 Nrap protein domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 79
Acc36I ACCTGC 2 cut(s) 79, 567
Acc65I GGTACC 1 cut(s) 345
AccB1I GGYRCC 3 cut(s) 345, 360, 1661
AccB7I CCANNNNNTGG 1 cut(s) 2207
AccI GTMKAC 1 cut(s) 90
AccII CGCG 1 cut(s) 579
AccIII TCCGGA 1 cut(s) 447
AciI CCGC 8 cut(s) 20, 240, 565, 739, 1823, 1886, 2897, 3130
AcoI YGGCCR 1 cut(s) 1883
AcsI RAATTY 8 cut(s) 203, 869, 1374, 1454, 1710, 2061, 2564, 2575
AcuI CTGAAG 4 cut(s) 47, 743, 867, 1964
AcvI CACGTG 1 cut(s) 2798
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 9 cut(s) 347, 368, 389, 620, 988, 1290, 2005, 2150, 2933
AfiI CCNNNNNNNGG 7 cut(s) 31, 1542, 1780, 1881, 1888, 2207, 3129
AflIII ACRYGT 4 cut(s) 642, 1291, 1552, 2000
AgeI ACCGGT 1 cut(s) 342
AhdI GACNNNNNGTC 1 cut(s) 1556
AjnI CCWGG 5 cut(s) 496, 614, 1121, 1298, 2836
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
Alw21I GWGCWC 2 cut(s) 42, 1388
Alw26I GTCTC 2 cut(s) 2041, 2086
AlwNI CAGNNNCTG 1 cut(s) 2012
Ama87I CYCGRG 1 cut(s) 1040
Aor13HI TCCGGA 1 cut(s) 447
AoxI GGCC 6 cut(s) 922, 1770, 1883, 1913, 2077, 3019
ApeKI GCWGC 4 cut(s) 116, 562, 1045, 2240
ApoI RAATTY 8 cut(s) 203, 869, 1374, 1454, 1710, 2061, 2564, 2575
AsiGI ACCGGT 1 cut(s) 342
Asp700I GAANNNNTTC 3 cut(s) 969, 1323, 1743
Asp718I GGTACC 1 cut(s) 345
AspLEI GCGC 3 cut(s) 76, 581, 1731
AspS9I GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2391, 2559, 2650, 3019
AsuC2I CCSGG 5 cut(s) 168, 1019, 1277, 1687, 3023
AsuHPI GGTGA 5 cut(s) 60, 70, 1291, 2562, 2984
AsuII TTCGAA 1 cut(s) 2686
AsuNHI GCTAGC 1 cut(s) 1825
AvaI CYCGRG 1 cut(s) 1040
AvaII GGWCC 7 cut(s) 22, 52, 426, 1421, 2391, 2559, 2650
AxyI CCTNAGG 1 cut(s) 603
BaeI ACNNNNGTAYC 2 cut(s) 610, 643
BamHI GGATCC 1 cut(s) 3049
BanI GGYRCC 3 cut(s) 345, 360, 1661
BanII GRGCYC 4 cut(s) 42, 736, 906, 1388
BbrPI CACGTG 1 cut(s) 2798
BbsI GAAGAC 7 cut(s) 393, 645, 1289, 1746, 1795, 1970, 2048
Bbv12I GWGCWC 2 cut(s) 42, 1388
BbvI GCAGC 4 cut(s) 128, 574, 1032, 2227
BccI CCATC 8 cut(s) 128, 538, 1000, 1496, 2229, 2593, 2664, 2773
BceAI ACGGC 2 cut(s) 83, 168
BcgI CGANNNNNNTGC 4 cut(s) 836, 870, 1708, 1742
BciT130I CCWGG 5 cut(s) 498, 616, 1123, 1300, 2838
BciVI GTATCC 1 cut(s) 480
BcnI CCSGG 5 cut(s) 168, 1019, 1277, 1687, 3023
BcoDI GTCTC 2 cut(s) 2041, 2086
BfaI CTAG 6 cut(s) 59, 996, 1775, 1826, 2525, 2921
BfmI CTRYAG 3 cut(s) 252, 560, 1761
BfuAI ACCTGC 2 cut(s) 79, 567
BfuI GTATCC 1 cut(s) 480
BisI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2241
BlpI GCTNAGC 1 cut(s) 1482
BlsI GCNGC 7 cut(s) 118, 242, 564, 740, 1047, 1887, 2242
BmcAI AGTACT 2 cut(s) 389, 2933
Bme18I GGWCC 7 cut(s) 22, 52, 426, 1421, 2391, 2559, 2650
BmeRI GACNNNNNGTC 1 cut(s) 1556
BmeT110I CYCGRG 1 cut(s) 1040
BmgT120I GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2391, 2559, 2650, 3019
BmiI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2363, 3020, 3051
BmrI ACTGGG 1 cut(s) 2112
BmsI GCATC 6 cut(s) 735, 1417, 1887, 2555, 2698, 2965
BmtI GCTAGC 1 cut(s) 1829
BmuI ACTGGG 1 cut(s) 2112
BpiI GAAGAC 7 cut(s) 393, 645, 1289, 1746, 1795, 1970, 2048
BpmI CTGGAG 1 cut(s) 2859
Bpu10I CCTNAGC 2 cut(s) 1487, 2900
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 1 cut(s) 2686
BpuEI CTTGAG 2 cut(s) 128, 2930
BpuMI CCSGG 5 cut(s) 168, 1019, 1277, 1687, 3023
Bsa29I ATCGAT 1 cut(s) 123
BsaAI YACGTR 2 cut(s) 1292, 2798
BsaBI GATNNNNATC 1 cut(s) 2374
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 5 cut(s) 306, 496, 497, 614, 3125
BsaWI WCCGGW 2 cut(s) 342, 447
Bsc4I CCNNNNNNNGG 7 cut(s) 31, 1542, 1780, 1881, 1888, 2207, 3129
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 5 cut(s) 473, 1916, 2107, 2353, 2393
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 5 cut(s) 849, 873, 1547, 2523, 2818
Bse8I GATNNNNATC 1 cut(s) 2374
BseAI TCCGGA 1 cut(s) 447
BseBI CCWGG 5 cut(s) 498, 616, 1123, 1300, 2838
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 5 cut(s) 306, 496, 497, 614, 3125
BseJI GATNNNNATC 1 cut(s) 2374
BseLI CCNNNNNNNGG 7 cut(s) 31, 1542, 1780, 1881, 1888, 2207, 3129
BseMI GCAATG 5 cut(s) 849, 873, 1547, 2523, 2818
BseMII CTCAG 9 cut(s) 30, 264, 617, 1473, 1566, 2004, 2526, 2541, 2871
BseNI ACTGG 5 cut(s) 473, 1916, 2107, 2353, 2393
BseRI GAGGAG 4 cut(s) 50, 959, 1457, 2288
BseX3I CGGCCG 1 cut(s) 1883
BseXI GCAGC 4 cut(s) 128, 574, 1032, 2227
BseYI CCCAGC 1 cut(s) 2200
BsgI GTGCAG 2 cut(s) 210, 1787
Bsh1236I CGCG 1 cut(s) 579
Bsh1285I CGRYCG 2 cut(s) 1886, 3134
BshFI GGCC 6 cut(s) 924, 1772, 1885, 1915, 2079, 3021
BshNI GGYRCC 3 cut(s) 345, 360, 1661
BshTI ACCGGT 1 cut(s) 342
BshVI ATCGAT 1 cut(s) 123
BsiEI CGRYCG 2 cut(s) 1886, 3134
BsiHKAI GWGCWC 2 cut(s) 42, 1388
BsiHKCI CYCGRG 1 cut(s) 1040
BsiSI CCGG 8 cut(s) 167, 343, 448, 1018, 1276, 1686, 1882, 3022
BslI CCNNNNNNNGG 7 cut(s) 31, 1542, 1780, 1881, 1888, 2207, 3129
BsmAI GTCTC 2 cut(s) 2041, 2086
BsmBI CGTCTC 1 cut(s) 2086
BsmI GAATGC 4 cut(s) 543, 597, 1952, 2809
BsnI GGCC 6 cut(s) 924, 1772, 1885, 1915, 2079, 3021
BsoBI CYCGRG 1 cut(s) 1040
Bsp119I TTCGAA 1 cut(s) 2686
Bsp1286I GDGCHC 4 cut(s) 42, 736, 906, 1388
Bsp13I TCCGGA 1 cut(s) 447
Bsp1407I TGTACA 1 cut(s) 2003
Bsp1720I GCTNAGC 1 cut(s) 1482
BspACI CCGC 8 cut(s) 20, 240, 565, 739, 1823, 1886, 2897, 3130
BspANI GGCC 6 cut(s) 924, 1772, 1885, 1915, 2079, 3021
BspCNI CTCAG 9 cut(s) 29, 265, 616, 1474, 1567, 2005, 2525, 2542, 2870
BspDI ATCGAT 1 cut(s) 123
BspEI TCCGGA 1 cut(s) 447
BspFNI CGCG 1 cut(s) 579
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2363, 3020, 3051
BspMAI CTGCAG 1 cut(s) 564
BspMI ACCTGC 2 cut(s) 79, 567
BspOI GCTAGC 1 cut(s) 1829
BspT104I TTCGAA 1 cut(s) 2686
BspT107I GGYRCC 3 cut(s) 345, 360, 1661
BsrDI GCAATG 5 cut(s) 849, 873, 1547, 2523, 2818
BsrFI RCCGGY 1 cut(s) 342
BsrGI TGTACA 1 cut(s) 2003
BsrI ACTGG 5 cut(s) 473, 1916, 2107, 2353, 2393
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 5 cut(s) 306, 496, 497, 614, 3125
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 306, 3125
Bst2UI CCWGG 5 cut(s) 498, 616, 1123, 1300, 2838
Bst4CI ACNGT 4 cut(s) 1288, 1525, 1765, 2148
Bst6I CTCTTC 2 cut(s) 1172, 1715
BstACI GRCGYC 1 cut(s) 96
BstAUI TGTACA 1 cut(s) 2003
BstBAI YACGTR 2 cut(s) 1292, 2798
BstBI TTCGAA 1 cut(s) 2686
BstC8I GCNNGC 7 cut(s) 736, 1075, 1770, 1827, 2226, 2263, 2809
BstFNI CGCG 1 cut(s) 579
BstHHI GCGC 3 cut(s) 76, 581, 1731
BstMAI GTCTC 2 cut(s) 2041, 2086
BstMCI CGRYCG 2 cut(s) 1886, 3134
BstMWI GCNNNNNNNGC 2 cut(s) 161, 2552
BstNI CCWGG 5 cut(s) 498, 616, 1123, 1300, 2838
BstNSI RCATGY 7 cut(s) 646, 1077, 1312, 1556, 1583, 2004, 2117
BstSFI CTRYAG 3 cut(s) 252, 560, 1761
BstUI CGCG 1 cut(s) 579
BstV1I GCAGC 4 cut(s) 128, 574, 1032, 2227
BstV2I GAAGAC 7 cut(s) 393, 645, 1289, 1746, 1795, 1970, 2048
BstX2I RGATCY 5 cut(s) 286, 1669, 2863, 2917, 3049
BstYI RGATCY 5 cut(s) 286, 1669, 2863, 2917, 3049
BstZI CGGCCG 1 cut(s) 1883
Bsu15I ATCGAT 1 cut(s) 123
Bsu36I CCTNAGG 1 cut(s) 603
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 6 cut(s) 924, 1772, 1885, 1915, 2079, 3021
BsuTUI ATCGAT 1 cut(s) 123
BtsI GCAGTG 2 cut(s) 1361, 2623
BtsIMutI CAGTG 5 cut(s) 1361, 1770, 2010, 2346, 2623
BveI ACCTGC 2 cut(s) 79, 567
Cac8I GCNNGC 7 cut(s) 736, 1075, 1770, 1827, 2226, 2263, 2809
CaiI CAGNNNCTG 1 cut(s) 2012
CciI TCATGA 1 cut(s) 760
CfoI GCGC 3 cut(s) 76, 581, 1731
Cfr10I RCCGGY 1 cut(s) 342
Cfr13I GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2391, 2559, 2650, 3019
ClaI ATCGAT 1 cut(s) 123
CseI GACGC 1 cut(s) 104
Csp6I GTAC 9 cut(s) 346, 367, 388, 619, 987, 1289, 2004, 2149, 2932
CspAI ACCGGT 1 cut(s) 342
CviQI GTAC 9 cut(s) 346, 367, 388, 619, 987, 1289, 2004, 2149, 2932
DraI TTTAAA 2 cut(s) 961, 2848
DriI GACNNNNNGTC 1 cut(s) 1556
EaeI YGGCCR 1 cut(s) 1883
EagI CGGCCG 1 cut(s) 1883
Eam1104I CTCTTC 2 cut(s) 1172, 1715
Eam1105I GACNNNNNGTC 1 cut(s) 1556
EarI CTCTTC 2 cut(s) 1172, 1715
Ecl136II GAGCTC 2 cut(s) 40, 1386
EclXI CGGCCG 1 cut(s) 1883
Eco130I CCWWGG 2 cut(s) 306, 3125
Eco147I AGGCCT 1 cut(s) 2079
Eco24I GRGCYC 4 cut(s) 42, 736, 906, 1388
Eco32I GATATC 1 cut(s) 1572
Eco47I GGWCC 7 cut(s) 22, 52, 426, 1421, 2391, 2559, 2650
Eco52I CGGCCG 1 cut(s) 1883
Eco53kI GAGCTC 2 cut(s) 40, 1386
Eco57I CTGAAG 4 cut(s) 47, 743, 867, 1964
Eco72I CACGTG 1 cut(s) 2798
Eco81I CCTNAGG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 1040
EcoICRI GAGCTC 2 cut(s) 40, 1386
EcoO109I RGGNCCY 1 cut(s) 1771
EcoRII CCWGG 5 cut(s) 496, 614, 1121, 1298, 2836
EcoRV GATATC 1 cut(s) 1572
EcoT14I CCWWGG 2 cut(s) 306, 3125
EcoT22I ATGCAT 2 cut(s) 1079, 1626
EcoT38I GRGCYC 4 cut(s) 42, 736, 906, 1388
ErhI CCWWGG 2 cut(s) 306, 3125
Esp3I CGTCTC 1 cut(s) 2086
FauNDI CATATG 1 cut(s) 2206
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2241
FriOI GRGCYC 4 cut(s) 42, 736, 906, 1388
Fsp4HI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2241
FspBI CTAG 6 cut(s) 59, 996, 1775, 1826, 2525, 2921
GlaI GCGC 3 cut(s) 75, 580, 1730
GluI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2241
GsaI CCCAGC 1 cut(s) 2204
GsuI CTGGAG 1 cut(s) 2859
HaeIII GGCC 6 cut(s) 924, 1772, 1885, 1915, 2079, 3021
HapII CCGG 8 cut(s) 167, 343, 448, 1018, 1276, 1686, 1882, 3022
HgaI GACGC 1 cut(s) 104
HhaI GCGC 3 cut(s) 76, 581, 1731
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 3 cut(s) 74, 579, 1729
HinP1I GCGC 3 cut(s) 74, 579, 1729
HincII GTYRAC 3 cut(s) 2162, 2413, 2631
HindII GTYRAC 3 cut(s) 2162, 2413, 2631
HindIII AAGCTT 2 cut(s) 1000, 1398
HpaII CCGG 8 cut(s) 167, 343, 448, 1018, 1276, 1686, 1882, 3022
HphI GGTGA 5 cut(s) 60, 70, 1291, 2562, 2984
Hpy166II GTNNAC 8 cut(s) 91, 429, 2137, 2162, 2413, 2509, 2631, 2887
Hpy8I GTNNAC 8 cut(s) 91, 429, 2137, 2162, 2413, 2509, 2631, 2887
Hpy99I CGWCG 1 cut(s) 98
HpyCH4III ACNGT 4 cut(s) 1288, 1525, 1765, 2148
HpyCH4IV ACGT 2 cut(s) 1291, 2797
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 2552
HpySE526I ACGT 2 cut(s) 1291, 2797
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 3 cut(s) 74, 579, 1729
Kpn2I TCCGGA 1 cut(s) 447
KpnI GGTACC 1 cut(s) 349
LmnI GCTCC 5 cut(s) 37, 169, 532, 2335, 2584
Lsp1109I GCAGC 4 cut(s) 128, 574, 1032, 2227
LweI GCATC 6 cut(s) 735, 1417, 1887, 2555, 2698, 2965
MaeI CTAG 6 cut(s) 59, 996, 1775, 1826, 2525, 2921
MaeII ACGT 2 cut(s) 1291, 2797
MaeIII GTNAC 8 cut(s) 148, 475, 859, 1021, 1271, 1294, 1344, 2195
MfeI CAATTG 4 cut(s) 975, 1932, 2280, 3054
MflI RGATCY 5 cut(s) 286, 1669, 2863, 2917, 3049
MhlI GDGCHC 4 cut(s) 42, 736, 906, 1388
MlyI GAGTC 1 cut(s) 1984
MmeI TCCRAC 3 cut(s) 1012, 1202, 3064
Mph1103I ATGCAT 2 cut(s) 1079, 1626
MroI TCCGGA 1 cut(s) 447
MroXI GAANNNNTTC 3 cut(s) 969, 1323, 1743
MslI CAYNNNNRTG 1 cut(s) 1072
MspA1I CMGCKG 3 cut(s) 20, 565, 1888
MspI CCGG 8 cut(s) 167, 343, 448, 1018, 1276, 1686, 1882, 3022
MunI CAATTG 4 cut(s) 975, 1932, 2280, 3054
Mva1269I GAATGC 4 cut(s) 543, 597, 1952, 2809
MvaI CCWGG 5 cut(s) 498, 616, 1123, 1300, 2838
MvnI CGCG 1 cut(s) 579
MwoI GCNNNNNNNGC 2 cut(s) 161, 2552
NciI CCSGG 5 cut(s) 168, 1019, 1277, 1687, 3023
NdeI CATATG 1 cut(s) 2206
NheI GCTAGC 1 cut(s) 1825
NlaIV GGNNCC 7 cut(s) 347, 362, 818, 1663, 2363, 3020, 3051
NmuCI GTSAC 4 cut(s) 475, 1271, 1294, 1344
NsiI ATGCAT 2 cut(s) 1079, 1626
NspI RCATGY 7 cut(s) 646, 1077, 1312, 1556, 1583, 2004, 2117
NspV TTCGAA 1 cut(s) 2686
PaeI GCATGC 1 cut(s) 1077
PaeR7I CTCGAG 1 cut(s) 1040
PagI TCATGA 1 cut(s) 760
PaqCI CACCTGC 1 cut(s) 79
PasI CCCWGGG 1 cut(s) 497
PceI AGGCCT 1 cut(s) 2079
PciI ACATGT 3 cut(s) 642, 1552, 2000
PctI GAATGC 4 cut(s) 543, 597, 1952, 2809
PdmI GAANNNNTTC 3 cut(s) 969, 1323, 1743
PfeI GAWTC 9 cut(s) 171, 965, 1055, 1319, 1494, 1778, 2184, 2660, 2960
PflFI GACNNNGTC 1 cut(s) 1151
PflMI CCANNNNNTGG 1 cut(s) 2207
PfoI TCCNGGA 2 cut(s) 166, 1685
PinAI ACCGGT 1 cut(s) 342
PkrI GCNGC 7 cut(s) 118, 242, 564, 740, 1047, 1887, 2242
PleI GAGTC 1 cut(s) 1983
PmaCI CACGTG 1 cut(s) 2798
PmlI CACGTG 1 cut(s) 2798
PpsI GAGTC 1 cut(s) 1983
Ppu21I YACGTR 2 cut(s) 1292, 2798
PscI ACATGT 3 cut(s) 642, 1552, 2000
Psp124BI GAGCTC 2 cut(s) 42, 1388
Psp6I CCWGG 5 cut(s) 496, 614, 1121, 1298, 2836
PspCI CACGTG 1 cut(s) 2798
PspFI CCCAGC 1 cut(s) 2200
PspGI CCWGG 5 cut(s) 496, 614, 1121, 1298, 2836
PspN4I GGNNCC 7 cut(s) 347, 362, 818, 1663, 2363, 3020, 3051
PspPI GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2391, 2559, 2650, 3019
PstI CTGCAG 1 cut(s) 564
PstNI CAGNNNCTG 1 cut(s) 2012
PsuI RGATCY 5 cut(s) 286, 1669, 2863, 2917, 3049
PsyI GACNNNGTC 1 cut(s) 1151
RsaI GTAC 9 cut(s) 347, 368, 389, 620, 988, 1290, 2005, 2150, 2933
RsaNI GTAC 9 cut(s) 346, 367, 388, 619, 987, 1289, 2004, 2149, 2932
RseI CAYNNNNRTG 1 cut(s) 1072
SacI GAGCTC 2 cut(s) 42, 1388
SatI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2241
Sau96I GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2391, 2559, 2650, 3019
ScaI AGTACT 2 cut(s) 389, 2933
SchI GAGTC 1 cut(s) 1984
SduI GDGCHC 4 cut(s) 42, 736, 906, 1388
SfaNI GCATC 6 cut(s) 735, 1417, 1887, 2555, 2698, 2965
SfcI CTRYAG 3 cut(s) 252, 560, 1761
Sfr274I CTCGAG 1 cut(s) 1040
SfuI TTCGAA 1 cut(s) 2686
SinI GGWCC 7 cut(s) 22, 52, 426, 1421, 2391, 2559, 2650
SlaI CTCGAG 1 cut(s) 1040
SmiMI CAYNNNNRTG 1 cut(s) 1072
SmlI CTYRAG 3 cut(s) 143, 1040, 2945
SmoI CTYRAG 3 cut(s) 143, 1040, 2945
SphI GCATGC 1 cut(s) 1077
SseBI AGGCCT 1 cut(s) 2079
SsiI CCGC 8 cut(s) 20, 240, 565, 739, 1823, 1886, 2897, 3130
SspI AATATT 2 cut(s) 65, 852
SspMI CTAG 6 cut(s) 59, 996, 1775, 1826, 2525, 2921
SstI GAGCTC 2 cut(s) 42, 1388
StuI AGGCCT 1 cut(s) 2079
StyI CCWWGG 2 cut(s) 306, 3125
TaaI ACNGT 4 cut(s) 1288, 1525, 1765, 2148
TaiI ACGT 2 cut(s) 1294, 2800
TaqI TCGA 6 cut(s) 107, 123, 1041, 1607, 1718, 2686
TatI WGTACW 5 cut(s) 387, 986, 2003, 2148, 2931
TauI GCSGC 3 cut(s) 243, 741, 1888
TfiI GAWTC 9 cut(s) 171, 965, 1055, 1319, 1494, 1778, 2184, 2660, 2960
TscAI CASTG 5 cut(s) 1368, 1770, 2017, 2353, 2623
TseFI GTSAC 4 cut(s) 475, 1271, 1294, 1344
TseI GCWGC 4 cut(s) 116, 562, 1045, 2240
Tsp45I GTSAC 4 cut(s) 475, 1271, 1294, 1344
TspGWI ACGGA 2 cut(s) 1448, 2356
TspRI CASTG 5 cut(s) 1368, 1770, 2017, 2353, 2623
Tth111I GACNNNGTC 1 cut(s) 1151
Van91I CCANNNNNTGG 1 cut(s) 2207
VpaK11BI GGWCC 7 cut(s) 22, 52, 426, 1421, 2391, 2559, 2650
XapI RAATTY 8 cut(s) 203, 869, 1374, 1454, 1710, 2061, 2564, 2575
XbaI TCTAGA 2 cut(s) 995, 2920
XceI RCATGY 7 cut(s) 646, 1077, 1312, 1556, 1583, 2004, 2117
XcmI CCANNNNNNNNNTGG 1 cut(s) 2641
XhoI CTCGAG 1 cut(s) 1040
XmiI GTMKAC 1 cut(s) 90
XmnI GAANNNNTTC 3 cut(s) 969, 1323, 1743
XspI CTAG 6 cut(s) 59, 996, 1775, 1826, 2525, 2921
ZrmI AGTACT 2 cut(s) 389, 2933
Zsp2I ATGCAT 2 cut(s) 1079, 1626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.