Rh2BG152300

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0008672
Physical Location & Seq
Forward (+)
13073735 .. 13082062
8328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG152300.1

Sequence Viewer

Length: 3081 bp
ATGGAAACCGATAACTCAGCGGACCTGAAGGTGGAGGAGCTCTTGAAAGAGGTCCAGCTAGAATATTCGCCTGCTCTCACCAAGTTCGTAGACGACGCCGTTTCATCGATCAAAGCAGCCATCGATACAATTCCACAAGACCTCAAGGTTACGGCTGATTTAGCTCCGGGATTCGTCAGAGATATTGGTGCAGACAAAGTTGAATTTGATTTCAAGAAGCCGAAATCTGTCAAAATTGGCGGCAGTTATGCTATAGAGTGCCTTGTAAAGCCTGAGTTCAATGTGGATCTTTTCGTTCAGTTGCCCAAGGAATGTTTCCATGAGAAAGACTATTTGAATTACCGGTACCATGCTAAAAGGTGCCTGTACCTTTGTGTGATTAAAAAGTACTTGATGTCGTCTTCGTTAGTTGGTAAGGTTGAATGGTCCACTTTCCAGAACGAAGTCCGGAAACCTGTGTTGATTGTGTATCCAGTCACGAAATTAGTTGCACTCCCTGGGTTCTTTGTGAGAATAATTCCCACAGCACCATCTCTTTTTAGCATTCCAAAATTGAACCTGCAGCGGAATAATGTTCGTGCTGTGAGTAAAGGGGGCATTCCTCAGTCTACTCCCAAGTACAATAGTAGTATTTTGGAAGACATGTTTATAGAAGATACAGAAAAACTTGTCAAGCAAACTTTTCTTGGGTCAAAGGAATTACGAGAAGGTTTAGTTTTGCTGAAGGTCTGGGCTCGCCGCAGGATACCAATATATGCTTATGATTGCCTGAATGGTTTTTTAATCTCAGTCATATTGGCATATCTTGTGGATAGGAACCATATCAATAAATCAATGAAGGCAATGCAAATATTTCGTGTCACAATGAAATTCATTGCTACTTCAGATTTGTGGAAACATGGGCTCTACTTTACACCAAAAGGCCAAAAAGCTATTTCAAAAGAGGAGAGGTTGCCTTTTAAAGAATCATTTCCAATTGTTATATGTACTCCATCTAGAAGCTTTAATTTAGCCTTCCGGGTAACAAGGGTTGGATTTCTCGAGCTGCAAAATGAATCTACAATGACACTGGCATGCATAGAGAAATGCAGAGACAGTGGATTTGAGGAAGTATTTATGACCAGGATTGACTATCCTGTAAAATATGACCATGTCTTAAGGTTGAACTTAAAGGGGAAGAGTTCAGTTTATGGATTGGGATTTTGCTTGGATGATGAATGTTGGAGAGTGTATGAGCAGAAGGTTCATAATGTTTTAAGTCATGGGTTGAGTGACCGAGTGAAGACTGTACGTGTGACCTGGAAAAGCATGTTATCAGAATCCATTCTACAGAATGGATTGTCGTCACTCAATACAGAGCCACTGCTAATTGGAATTTCAGTGAGCTCTTTAGATAAAGTTTTAAGAATTGTTGATATTGGTCCTGATGCTGATAATAAGGATGAGGCTCTCAAATTCCGTCAGTTCTGGGGGGATAAGGCTGAGCTTAGGAGATTCAAAGATGGCAAGATAGCAGAAAGCACAGTTTGGGAGACTGAGCAATGGAAAAGACATATCATCATGAAAGAGATATCTGAGCATGTGCTTCTGCGACATCTTTCACTATCAAAAGAGAATATCATGCATATCGTGGATCAACTTGATTTCTCTTTACTATATGGTGCCGAAGATCCTATATCTTCTTCTGGGAGTTTGTTTGGGGCGTTTGAAATTCTATCGAAGCGATTGCGCCTTATTGAAGACATTCCTCTAAAGGTTTCTACAGTGCAGGCCCTAGATTCGGCTTTCAGATATTCATCTGTCTTCCCACCTGAACCTCATCCGCTAGCTAATGAAAAGGGTTCTTTCGTAAGACTAAATCAGTTACCTCCTTCCTGCATCCGGCCGCTGGAAGTTATGATCCAGTTGGAAGGTTCTGGAAACTGGCCTATGGATGATGTAGCAATTGAGAAAACTAAATCAGCATTCCTTCTTCAAATCGGAGAGAGTCTTCAGAATAGTTGGGGGATGACATGTACAGCCACTGAGGATGATGTGGATGTTTTTGTCTCTGGATATGCCTTTCGTCTTAAAATTTGGCATGAGAGAGGCCTGACGTTGTTGAGACGAGAAACTGGGAATGAACACGTGAAGAAGGTTTCTAATGTGGACAAGGAACTATACTTTCGCAGTCAACATTCCAGCATGATAAATGGATTCCAGACTTGTTTCCCAGCATATGGGCCAGTTGTTAGGCTTGCAAAACGATGGGCTGCTTCACATCTATTTTCGGCTTGCTTGGTAGAGGAGGCAATTGAGCTATTGGTAGCATATATATTTTTGAAGCCTTTACCATTTAATGCTCCATGCTCACGGGTCACTGGTTTTTTAAGGTTCCTGCGATTACTATCAGATTATGACTGGACCTTTTCTGCATTAGTTGTTGACATAAATAATGATTTAACATCAAATGATGAGAAAGAGATAAGAGAGAACTTCATGTTAAGTAGAAAAGGTTATGAAGAAAATCCTCAAAATGTAAACTCAGCAATGTTCCTAGCTACATCCTACGACAAGGCATCTGAGGCTTGGACCAAATTTTCACCAAACTCAATGGAGCTAAAAAGGTTGATGGCTTATGCTGGAAGCAGTGCAAACTTGTTGACCAAACTGATATTGGAGGACCATAGTGATTCTTATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAATTATGATGCTGTAATTCTTCTCCATAGAGAAAAATTACCTTATCCCCAACGCCTTCTCTTTCCATCAGAATTACATCAAGGGGTTCATGTGGCACGTGGGAATGCGAGCAAGGGTTTCCACCCCTTTCTTTTGCCTGGAGATTTTAAAGGAAGCTCAGAGGATCTAAGAAACAAAGTATTGGTAAACTTCGATCCGCTGAGGTGTTTTGTTGGGGATCTAGAGGCAAGTATTATAGAATGCCTTGATTTATTTATTTATTATCATATGAAATCATTACTTCTTGTATTTGGTCGATGCTGTAGTTGCTCACATCCACTTTTCTTCAATGTAGAAAGAGTACTCAAACACTTTCAAGCTATGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

1026

Amino Acids

117.26

Weight (kDa)

8.17

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 95 - 227 3.1e-35 Nrap protein domain 1
Nrap_D2 PF17403 234 - 375 4.6e-31 Nrap protein PAP/OAS-like domain
Nrap_D3 PF17404 380 - 534 3.6e-37 Nrap protein domain 3
Nrap_D4 PF17405 556 - 735 8.6e-45 Nrap protein nucleotidyltransferase domain 4
Nrap_D5 PF17406 737 - 889 4.7e-47 Nrap protein PAP/OAS1-like domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 567
Acc65I GGTACC 1 cut(s) 345
AccB1I GGYRCC 3 cut(s) 345, 360, 1661
AccB7I CCANNNNNTGG 1 cut(s) 2219
AccI GTMKAC 2 cut(s) 90, 608
AccIII TCCGGA 1 cut(s) 447
AciI CCGC 7 cut(s) 20, 240, 565, 739, 1823, 1886, 2909
AclWI GGATC 7 cut(s) 294, 1641, 1664, 1894, 2883, 2900, 2937
AcoI YGGCCR 1 cut(s) 1883
AcsI RAATTY 7 cut(s) 203, 869, 1374, 1454, 1710, 2073, 2576
AcuI CTGAAG 4 cut(s) 47, 743, 867, 1976
AcvI CACGTG 2 cut(s) 2128, 2810
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 8 cut(s) 347, 368, 389, 620, 988, 1290, 2017, 3054
AfiI CCNNNNNNNGG 5 cut(s) 31, 1780, 1881, 1888, 2219
AflII CTTAAG 1 cut(s) 1156
AflIII ACRYGT 4 cut(s) 642, 1291, 2012, 2125
AgeI ACCGGT 1 cut(s) 342
AjnI CCWGG 4 cut(s) 496, 1121, 1298, 2848
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
Alw21I GWGCWC 2 cut(s) 42, 1388
Alw26I GTCTC 4 cut(s) 1086, 1526, 2053, 2098
AlwI GGATC 7 cut(s) 294, 1641, 1664, 1894, 2883, 2900, 2937
AlwNI CAGNNNCTG 1 cut(s) 2024
Ama87I CYCGRG 1 cut(s) 1040
Aor13HI TCCGGA 1 cut(s) 447
AoxI GGCC 6 cut(s) 922, 1770, 1883, 1925, 2089, 2222
ApeKI GCWGC 4 cut(s) 116, 562, 1045, 2252
ApoI RAATTY 7 cut(s) 203, 869, 1374, 1454, 1710, 2073, 2576
ArsI GACNNNNNNTTYG 2 cut(s) 1085, 1117
AsiGI ACCGGT 1 cut(s) 342
Asp700I GAANNNNTTC 3 cut(s) 969, 1323, 1743
Asp718I GGTACC 1 cut(s) 345
AspLEI GCGC 1 cut(s) 1731
AspS9I GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2222, 2403, 2571, 2662
AsuC2I CCSGG 2 cut(s) 168, 1019
AsuHPI GGTGA 2 cut(s) 70, 2574
AsuII TTCGAA 1 cut(s) 2698
AsuNHI GCTAGC 1 cut(s) 1825
AvaI CYCGRG 1 cut(s) 1040
AvaII GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
BanI GGYRCC 3 cut(s) 345, 360, 1661
BanII GRGCYC 4 cut(s) 42, 736, 906, 1388
BbrPI CACGTG 2 cut(s) 2128, 2810
BbsI GAAGAC 6 cut(s) 393, 645, 1289, 1746, 1795, 1982
Bbv12I GWGCWC 2 cut(s) 42, 1388
BbvCI CCTCAGC 1 cut(s) 2912
BbvI GCAGC 4 cut(s) 128, 574, 1032, 2239
BccI CCATC 8 cut(s) 128, 538, 1000, 1496, 2241, 2605, 2676, 2785
BceAI ACGGC 2 cut(s) 83, 168
BcgI CGANNNNNNTGC 4 cut(s) 836, 870, 1708, 1742
BciT130I CCWGG 4 cut(s) 498, 1123, 1300, 2850
BciVI GTATCC 2 cut(s) 480, 738
BcnI CCSGG 2 cut(s) 168, 1019
BcoDI GTCTC 4 cut(s) 1086, 1526, 2053, 2098
BfaI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
BfmI CTRYAG 5 cut(s) 252, 560, 1328, 1761, 3013
BfrI CTTAAG 1 cut(s) 1156
BfuAI ACCTGC 1 cut(s) 567
BfuI GTATCC 2 cut(s) 480, 738
BisI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
BlpI GCTNAGC 1 cut(s) 1482
BlsI GCNGC 7 cut(s) 118, 242, 564, 740, 1047, 1887, 2254
BmcAI AGTACT 2 cut(s) 389, 3054
Bme1390I CCNGG 6 cut(s) 168, 498, 1019, 1123, 1300, 2850
Bme18I GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
BmeT110I CYCGRG 1 cut(s) 1040
BmgT120I GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2222, 2403, 2571, 2662
BmiI GGNNCC 5 cut(s) 347, 362, 818, 1663, 2375
BmrFI CCNGG 6 cut(s) 168, 498, 1019, 1123, 1300, 2850
BmrI ACTGGG 1 cut(s) 2124
BmsI GCATC 5 cut(s) 1417, 1887, 2567, 2710, 2999
BmtI GCTAGC 1 cut(s) 1829
BmuI ACTGGG 1 cut(s) 2124
BpiI GAAGAC 6 cut(s) 393, 645, 1289, 1746, 1795, 1982
BpmI CTGGAG 1 cut(s) 2871
Bpu10I CCTNAGC 2 cut(s) 1487, 2912
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 1 cut(s) 2698
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 2 cut(s) 168, 1019
Bsa29I ATCGAT 2 cut(s) 107, 123
BsaAI YACGTR 3 cut(s) 1292, 2128, 2810
BsaBI GATNNNNATC 2 cut(s) 1795, 2386
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 306, 496, 497
BsaWI WCCGGW 2 cut(s) 342, 447
Bsc4I CCNNNNNNNGG 5 cut(s) 31, 1780, 1881, 1888, 2219
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 8 cut(s) 473, 1074, 1903, 1928, 2119, 2225, 2365, 2405
Bse3DI GCAATG 4 cut(s) 849, 873, 1547, 2535
Bse8I GATNNNNATC 2 cut(s) 1795, 2386
BseAI TCCGGA 1 cut(s) 447
BseBI CCWGG 4 cut(s) 498, 1123, 1300, 2850
BseCI ATCGAT 2 cut(s) 107, 123
BseDI CCNNGG 3 cut(s) 306, 496, 497
BseJI GATNNNNATC 2 cut(s) 1795, 2386
BseLI CCNNNNNNNGG 5 cut(s) 31, 1780, 1881, 1888, 2219
BseMI GCAATG 4 cut(s) 849, 873, 1547, 2535
BseNI ACTGG 8 cut(s) 473, 1074, 1903, 1928, 2119, 2225, 2365, 2405
BseRI GAGGAG 3 cut(s) 50, 959, 2300
BseX3I CGGCCG 1 cut(s) 1883
BseXI GCAGC 4 cut(s) 128, 574, 1032, 2239
BseYI CCCAGC 1 cut(s) 2212
BsgI GTGCAG 2 cut(s) 210, 1787
Bsh1285I CGRYCG 1 cut(s) 1886
BshFI GGCC 6 cut(s) 924, 1772, 1885, 1927, 2091, 2224
BshNI GGYRCC 3 cut(s) 345, 360, 1661
BshTI ACCGGT 1 cut(s) 342
BshVI ATCGAT 2 cut(s) 107, 123
BsiEI CGRYCG 1 cut(s) 1886
BsiHKAI GWGCWC 2 cut(s) 42, 1388
BsiHKCI CYCGRG 1 cut(s) 1040
BsiSI CCGG 5 cut(s) 167, 343, 448, 1018, 1882
BslI CCNNNNNNNGG 5 cut(s) 31, 1780, 1881, 1888, 2219
BsmAI GTCTC 4 cut(s) 1086, 1526, 2053, 2098
BsmBI CGTCTC 1 cut(s) 2098
BsmI GAATGC 5 cut(s) 543, 597, 1964, 2821, 2957
BsnI GGCC 6 cut(s) 924, 1772, 1885, 1927, 2091, 2224
BsoBI CYCGRG 1 cut(s) 1040
Bsp119I TTCGAA 1 cut(s) 2698
Bsp1286I GDGCHC 4 cut(s) 42, 736, 906, 1388
Bsp13I TCCGGA 1 cut(s) 447
Bsp1407I TGTACA 1 cut(s) 2015
Bsp143I GATC 8 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929
Bsp1720I GCTNAGC 1 cut(s) 1482
BspACI CCGC 7 cut(s) 20, 240, 565, 739, 1823, 1886, 2909
BspANI GGCC 6 cut(s) 924, 1772, 1885, 1927, 2091, 2224
BspDI ATCGAT 2 cut(s) 107, 123
BspEI TCCGGA 1 cut(s) 447
BspHI TCATGA 1 cut(s) 1560
BspLI GGNNCC 5 cut(s) 347, 362, 818, 1663, 2375
BspMAI CTGCAG 1 cut(s) 564
BspMI ACCTGC 1 cut(s) 567
BspOI GCTAGC 1 cut(s) 1829
BspPI GGATC 7 cut(s) 294, 1641, 1664, 1894, 2883, 2900, 2937
BspT104I TTCGAA 1 cut(s) 2698
BspT107I GGYRCC 3 cut(s) 345, 360, 1661
BspTI CTTAAG 1 cut(s) 1156
BsrDI GCAATG 4 cut(s) 849, 873, 1547, 2535
BsrFI RCCGGY 1 cut(s) 342
BsrGI TGTACA 1 cut(s) 2015
BsrI ACTGG 8 cut(s) 473, 1074, 1903, 1928, 2119, 2225, 2365, 2405
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 3 cut(s) 306, 496, 497
BssMI GATC 8 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 4 cut(s) 498, 1123, 1300, 2850
Bst4CI ACNGT 4 cut(s) 1097, 1288, 1525, 1765
Bst6I CTCTTC 1 cut(s) 1172
BstACI GRCGYC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 1156
BstAUI TGTACA 1 cut(s) 2015
BstBAI YACGTR 3 cut(s) 1292, 2128, 2810
BstBI TTCGAA 1 cut(s) 2698
BstC8I GCNNGC 8 cut(s) 72, 736, 1075, 1770, 1827, 2238, 2275, 2821
BstHHI GCGC 1 cut(s) 1731
BstKTI GATC 8 cut(s) 111, 289, 1636, 1672, 1902, 2878, 2908, 2932
BstMAI GTCTC 4 cut(s) 1086, 1526, 2053, 2098
BstMBI GATC 8 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929
BstMCI CGRYCG 1 cut(s) 1886
BstMWI GCNNNNNNNGC 3 cut(s) 161, 2564, 3018
BstNI CCWGG 4 cut(s) 498, 1123, 1300, 2850
BstNSI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2016
BstSCI CCNGG 6 cut(s) 166, 496, 1017, 1121, 1298, 2848
BstSFI CTRYAG 5 cut(s) 252, 560, 1328, 1761, 3013
BstV1I GCAGC 4 cut(s) 128, 574, 1032, 2239
BstV2I GAAGAC 6 cut(s) 393, 645, 1289, 1746, 1795, 1982
BstX2I RGATCY 4 cut(s) 286, 1669, 2875, 2929
BstYI RGATCY 4 cut(s) 286, 1669, 2875, 2929
BstZI CGGCCG 1 cut(s) 1883
Bsu15I ATCGAT 2 cut(s) 107, 123
BsuI GTATCC 2 cut(s) 480, 738
BsuRI GGCC 6 cut(s) 924, 1772, 1885, 1927, 2091, 2224
BsuTUI ATCGAT 2 cut(s) 107, 123
BtsI GCAGTG 2 cut(s) 1361, 2635
BtsIMutI CAGTG 8 cut(s) 1067, 1102, 1361, 1386, 1770, 2022, 2358, 2635
BveI ACCTGC 1 cut(s) 567
Cac8I GCNNGC 8 cut(s) 72, 736, 1075, 1770, 1827, 2238, 2275, 2821
CaiI CAGNNNCTG 1 cut(s) 2024
CciI TCATGA 1 cut(s) 1560
CfoI GCGC 1 cut(s) 1731
Cfr10I RCCGGY 1 cut(s) 342
Cfr13I GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2222, 2403, 2571, 2662
ClaI ATCGAT 2 cut(s) 107, 123
CseI GACGC 1 cut(s) 104
Csp6I GTAC 8 cut(s) 346, 367, 388, 619, 987, 1289, 2016, 3053
CspAI ACCGGT 1 cut(s) 342
CviQI GTAC 8 cut(s) 346, 367, 388, 619, 987, 1289, 2016, 3053
DpnI GATC 8 cut(s) 110, 288, 1635, 1671, 1901, 2877, 2907, 2931
DpnII GATC 8 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929
DraI TTTAAA 2 cut(s) 961, 2860
EaeI YGGCCR 1 cut(s) 1883
EagI CGGCCG 1 cut(s) 1883
Eam1104I CTCTTC 1 cut(s) 1172
EarI CTCTTC 1 cut(s) 1172
Ecl136II GAGCTC 2 cut(s) 40, 1386
EclXI CGGCCG 1 cut(s) 1883
Eco130I CCWWGG 1 cut(s) 306
Eco147I AGGCCT 1 cut(s) 2091
Eco24I GRGCYC 4 cut(s) 42, 736, 906, 1388
Eco32I GATATC 1 cut(s) 1572
Eco47I GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
Eco52I CGGCCG 1 cut(s) 1883
Eco53kI GAGCTC 2 cut(s) 40, 1386
Eco57I CTGAAG 4 cut(s) 47, 743, 867, 1976
Eco72I CACGTG 2 cut(s) 2128, 2810
Eco88I CYCGRG 1 cut(s) 1040
EcoICRI GAGCTC 2 cut(s) 40, 1386
EcoO109I RGGNCCY 1 cut(s) 1771
EcoRII CCWGG 4 cut(s) 496, 1121, 1298, 2848
EcoRV GATATC 1 cut(s) 1572
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 2 cut(s) 1079, 1626
EcoT38I GRGCYC 4 cut(s) 42, 736, 906, 1388
ErhI CCWWGG 1 cut(s) 306
Esp3I CGTCTC 1 cut(s) 2098
FauNDI CATATG 2 cut(s) 2218, 2979
FblI GTMKAC 2 cut(s) 90, 608
Fnu4HI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
FriOI GRGCYC 4 cut(s) 42, 736, 906, 1388
Fsp4HI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
FspBI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
GlaI GCGC 1 cut(s) 1730
GluI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
GsaI CCCAGC 1 cut(s) 2216
GsuI CTGGAG 1 cut(s) 2871
HaeIII GGCC 6 cut(s) 924, 1772, 1885, 1927, 2091, 2224
HapII CCGG 5 cut(s) 167, 343, 448, 1018, 1882
HgaI GACGC 1 cut(s) 104
HhaI GCGC 1 cut(s) 1731
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 1 cut(s) 1729
HinP1I GCGC 1 cut(s) 1729
HincII GTYRAC 3 cut(s) 2174, 2425, 2643
HindII GTYRAC 3 cut(s) 2174, 2425, 2643
HindIII AAGCTT 1 cut(s) 1000
HinfI GANTC 9 cut(s) 171, 965, 1055, 1319, 1494, 1778, 1987, 2196, 2672
HpaII CCGG 5 cut(s) 167, 343, 448, 1018, 1882
HphI GGTGA 2 cut(s) 70, 2574
Hpy166II GTNNAC 9 cut(s) 91, 429, 609, 2149, 2174, 2425, 2521, 2643, 2899
Hpy8I GTNNAC 9 cut(s) 91, 429, 609, 2149, 2174, 2425, 2521, 2643, 2899
Hpy99I CGWCG 1 cut(s) 98
HpyCH4III ACNGT 4 cut(s) 1097, 1288, 1525, 1765
HpyCH4IV ACGT 4 cut(s) 1291, 2096, 2127, 2809
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 2564, 3018
HpySE526I ACGT 4 cut(s) 1291, 2096, 2127, 2809
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 1 cut(s) 1729
Kpn2I TCCGGA 1 cut(s) 447
KpnI GGTACC 1 cut(s) 349
Kzo9I GATC 8 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929
LmnI GCTCC 4 cut(s) 37, 169, 2347, 2596
Lsp1109I GCAGC 4 cut(s) 128, 574, 1032, 2239
LweI GCATC 5 cut(s) 1417, 1887, 2567, 2710, 2999
MaeI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
MaeII ACGT 4 cut(s) 1291, 2096, 2127, 2809
MaeIII GTNAC 9 cut(s) 148, 475, 859, 1021, 1271, 1294, 1344, 1863, 2356
MalI GATC 8 cut(s) 110, 288, 1635, 1671, 1901, 2877, 2907, 2931
MboI GATC 8 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929
MfeI CAATTG 3 cut(s) 975, 1944, 2292
MflI RGATCY 4 cut(s) 286, 1669, 2875, 2929
MhlI GDGCHC 4 cut(s) 42, 736, 906, 1388
MlyI GAGTC 1 cut(s) 1996
MmeI TCCRAC 3 cut(s) 1012, 1202, 1887
Mph1103I ATGCAT 2 cut(s) 1079, 1626
MroI TCCGGA 1 cut(s) 447
MroXI GAANNNNTTC 3 cut(s) 969, 1323, 1743
MslI CAYNNNNRTG 1 cut(s) 1072
MspA1I CMGCKG 4 cut(s) 20, 565, 1888, 2911
MspCI CTTAAG 1 cut(s) 1156
MspI CCGG 5 cut(s) 167, 343, 448, 1018, 1882
MspR9I CCNGG 6 cut(s) 168, 498, 1019, 1123, 1300, 2850
MunI CAATTG 3 cut(s) 975, 1944, 2292
Mva1269I GAATGC 5 cut(s) 543, 597, 1964, 2821, 2957
MvaI CCWGG 4 cut(s) 498, 1123, 1300, 2850
MwoI GCNNNNNNNGC 3 cut(s) 161, 2564, 3018
NciI CCSGG 2 cut(s) 168, 1019
NdeI CATATG 2 cut(s) 2218, 2979
NdeII GATC 8 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929
NheI GCTAGC 1 cut(s) 1825
NlaIV GGNNCC 5 cut(s) 347, 362, 818, 1663, 2375
NmuCI GTSAC 6 cut(s) 475, 859, 1271, 1294, 1344, 2356
NsiI ATGCAT 2 cut(s) 1079, 1626
NspI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2016
NspV TTCGAA 1 cut(s) 2698
PaeI GCATGC 1 cut(s) 1077
PaeR7I CTCGAG 1 cut(s) 1040
PagI TCATGA 1 cut(s) 1560
PasI CCCWGGG 1 cut(s) 497
PceI AGGCCT 1 cut(s) 2091
PciI ACATGT 2 cut(s) 642, 2012
PctI GAATGC 5 cut(s) 543, 597, 1964, 2821, 2957
PdmI GAANNNNTTC 3 cut(s) 969, 1323, 1743
PfeI GAWTC 8 cut(s) 171, 965, 1055, 1319, 1494, 1778, 2196, 2672
PflFI GACNNNGTC 1 cut(s) 1151
PflMI CCANNNNNTGG 1 cut(s) 2219
PfoI TCCNGGA 1 cut(s) 166
PinAI ACCGGT 1 cut(s) 342
PkrI GCNGC 7 cut(s) 118, 242, 564, 740, 1047, 1887, 2254
PleI GAGTC 1 cut(s) 1995
PmaCI CACGTG 2 cut(s) 2128, 2810
PmlI CACGTG 2 cut(s) 2128, 2810
PpsI GAGTC 1 cut(s) 1995
Ppu21I YACGTR 3 cut(s) 1292, 2128, 2810
PscI ACATGT 2 cut(s) 642, 2012
Psp124BI GAGCTC 2 cut(s) 42, 1388
Psp6I CCWGG 4 cut(s) 496, 1121, 1298, 2848
PspCI CACGTG 2 cut(s) 2128, 2810
PspFI CCCAGC 1 cut(s) 2212
PspGI CCWGG 4 cut(s) 496, 1121, 1298, 2848
PspN4I GGNNCC 5 cut(s) 347, 362, 818, 1663, 2375
PspPI GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2222, 2403, 2571, 2662
PstI CTGCAG 1 cut(s) 564
PstNI CAGNNNCTG 1 cut(s) 2024
PsuI RGATCY 4 cut(s) 286, 1669, 2875, 2929
PsyI GACNNNGTC 1 cut(s) 1151
RsaI GTAC 8 cut(s) 347, 368, 389, 620, 988, 1290, 2017, 3054
RsaNI GTAC 8 cut(s) 346, 367, 388, 619, 987, 1289, 2016, 3053
RseI CAYNNNNRTG 1 cut(s) 1072
SacI GAGCTC 2 cut(s) 42, 1388
SatI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
Sau3AI GATC 8 cut(s) 108, 286, 1633, 1669, 1899, 2875, 2905, 2929
Sau96I GGNCC 9 cut(s) 22, 52, 426, 1421, 1771, 2222, 2403, 2571, 2662
ScaI AGTACT 2 cut(s) 389, 3054
SchI GAGTC 1 cut(s) 1996
ScrFI CCNGG 6 cut(s) 168, 498, 1019, 1123, 1300, 2850
SduI GDGCHC 4 cut(s) 42, 736, 906, 1388
SfaNI GCATC 5 cut(s) 1417, 1887, 2567, 2710, 2999
SfcI CTRYAG 5 cut(s) 252, 560, 1328, 1761, 3013
Sfr274I CTCGAG 1 cut(s) 1040
SfuI TTCGAA 1 cut(s) 2698
SinI GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
SlaI CTCGAG 1 cut(s) 1040
SmiMI CAYNNNNRTG 1 cut(s) 1072
SmlI CTYRAG 3 cut(s) 143, 1040, 1156
SmoI CTYRAG 3 cut(s) 143, 1040, 1156
SphI GCATGC 1 cut(s) 1077
SseBI AGGCCT 1 cut(s) 2091
SsiI CCGC 7 cut(s) 20, 240, 565, 739, 1823, 1886, 2909
SspI AATATT 2 cut(s) 65, 852
SspMI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
SstI GAGCTC 2 cut(s) 42, 1388
StuI AGGCCT 1 cut(s) 2091
StyD4I CCNGG 6 cut(s) 166, 496, 1017, 1121, 1298, 2848
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 4 cut(s) 1097, 1288, 1525, 1765
TaiI ACGT 4 cut(s) 1294, 2099, 2130, 2812
TaqI TCGA 7 cut(s) 107, 123, 1041, 1718, 2698, 2904, 3007
TaqII GACCGA 1 cut(s) 1290
TatI WGTACW 5 cut(s) 387, 618, 986, 2015, 3052
TauI GCSGC 3 cut(s) 243, 741, 1888
TfiI GAWTC 8 cut(s) 171, 965, 1055, 1319, 1494, 1778, 2196, 2672
TscAI CASTG 8 cut(s) 1074, 1102, 1368, 1386, 1770, 2029, 2365, 2635
TseFI GTSAC 6 cut(s) 475, 859, 1271, 1294, 1344, 2356
TseI GCWGC 4 cut(s) 116, 562, 1045, 2252
Tsp45I GTSAC 6 cut(s) 475, 859, 1271, 1294, 1344, 2356
TspGWI ACGGA 1 cut(s) 1448
TspRI CASTG 8 cut(s) 1074, 1102, 1368, 1386, 1770, 2029, 2365, 2635
Tth111I GACNNNGTC 1 cut(s) 1151
Van91I CCANNNNNTGG 1 cut(s) 2219
Vha464I CTTAAG 1 cut(s) 1156
VpaK11BI GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
XapI RAATTY 7 cut(s) 203, 869, 1374, 1454, 1710, 2073, 2576
XbaI TCTAGA 2 cut(s) 995, 2932
XceI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2016
XcmI CCANNNNNNNNNTGG 1 cut(s) 2653
XhoI CTCGAG 1 cut(s) 1040
XmiI GTMKAC 2 cut(s) 90, 608
XmnI GAANNNNTTC 3 cut(s) 969, 1323, 1743
XspI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
ZrmI AGTACT 2 cut(s) 389, 3054
Zsp2I ATGCAT 2 cut(s) 1079, 1626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.