RLG00000017080

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008672
Physical Location & Seq
Forward (+)
12657262 .. 12665175
7914 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017080

Sequence Viewer

Length: 3150 bp
ATGGAAACCGATAACTCAGCGGACCTGAAGGTGGAGGAGCTCTTGAAAGAGGTCCAGCTAGAATATTCACCCGCGCTCACCAAGTTCGTAGACGACGCCGTTTCATCGATCAAAGCAGCCATCGGTACAATTCCACAAGACCTCAAGGTTACGGCTGATTTAGCTCCGGGATTCGTCAGAGATATTGGTGCAGACAAAGTTGAATTCGATTTCAAGAAGCCGAAATCTGTCAAAATTGGCGGCAGTTATGCTATAGAGTGCCTTGTAAAGCCTGAGTTCAATGTGGATCTTTTCGTTCAGTTGCCCAAGGAATGTTTCCATGAGAAAGACTATTTGAATTACCGGTACCATGCTAAAAGGTGCCTGTACCTTTGTGTGATTAAGAAGTACTTGATCTCGTCGTCGTTAGTTGGTAAGGTTGAATGGTCCACTTTCCAGAATGAAGTCCGGAAACCTGTGTTGATTGTGTATCCAGTCACGAAATTAGTTGCACTCCCTGGGTTCTTTGTGAGAATAATTCCCACAGCACCATCTCTTTTTAGCATTCCAAAATTAAACCTGCAGCGGAATAATGTTCGTGCTGTGAGTAAAGGGGGCATTCCTCAGTCTACTCCCAAGTACAATAGTAGTATTTTGGAAGACATGTTTATAGAAGATACAGAAGAACTTGTCAAGCAAACTTTTCTTGGGTCAAAGGAATTACGAGAAGGTTTAGTTTTGCTGAAGGTCTGGGCTCGCCGCAGGATGCCAATATATGCTTATGATTGCCTGAATGGTTTTCTAATCTCTGTCATATTGGCATACCTTGTGGATAGGAACCATATCAATAAATCAATGAAGGCAATGCAAATATTTCGTGTTACAATGAAATTCATTGCTACTTCAGATTTGTGGAAACATGGGCTCTACTTCACACCAAAAGGCCAAAAAGCTATTTCAAAAGAGGAGAGGTTGCCTTTTAAAGAATCATTTCCAATTGTTATATGTACTCCATCTAGAAGCTTTAATTTAGCCTTCCGGGTAACAAGGGTTGGATTTCTTGAGCTGCAAAATGAATCTACAATGACACTGGCATGCATAGAGAAATGCAGAGATAGTGGATTTGAGGAAGTATTTATGACCAGGATTGACTATCCTGTAAAATATGACCATGTCATAAGGTTGAACTTAAAGGGGAAGAGTTCAGTTTATGGATCGGGATTTTGCTTGGATGATGAATGTTGGAGAGTGTACGAGCAGAAGGTTCATAATGTTTTAAGTCATGGGTTGAGTGACCGAGTGAAGACTGTACGTGTGACCTGGAAAAGCATATTATCAGAATCCATTCTACAAAATGGATTGTTGTCACTCAATACAGAGCCACTGCTAATTGGAATTTCAGTGAGCTCTTTAGATAAAGCCTTAAGAATTGTTGACATTGGTCCTGATGCTGATGATAAGAATGAGGCTCTCAAATTCCGTCAGTTCTGGGGGGATAAGGCTGAGCTTAGGAGATTCAAAGATGGCAAGATAGCAGAAAGCACAGTTTGGGAAACTGAGCAATGGAAAAGACACATCGTCATAAAAAAGATATCTGAGCATGTGCTTCTGCGACATCTTTCACTATCAAAAGAGAATATCATGCATATCGTGGATCAACTTGATTTCTCTTTACTATATGGTGCCGAAGATCCTATATCTTCTTCCGGAAGTTTGTTTGGGGCGTTTGAAATTCTATCGAAGCGATTGCGCCTTATTGAAGACATTCCTCTAAAGGTTTCTACAGTGCAGGCCCTAGATTCGGCTTTCAGGTGTTCATCTGTCTTCCCACCTGAACCTCATCCGCTAGCTAATGAAAAGGGTTCTTTCGTAAGACTGAATCAGTTCCCTCCTTCCTGCATCCGGCCGCTGGAAGTTATGATCCAGTTGGAAGGTTCTGGAAACTGGCCTATGGATGATGTAGCCATTGAGAAAACTAAATCAGCATTCCTTCTTAAAATCGGAGAGAGTCTTCAGAATAGTTGGGGGATGACATGTACAGCCACTGAGGATGATGTGGATGTTTTTGTCTCTGGATATGCCTTTCGTCTTAAAATTTGGCATGAAAGAGGCCTGACTTTGTTGAGACGTGAAACTGGGAATGAACACGTGAAGAAGGTTTCTAATGTGGACAAGGAACTATACTTTCGCAGTCAACATTCCAGCATGATAAATGGATTCCAGACTTGTTACCCAGCATATGGGCCGGTTGTTAGGCTTGCAAAACGATGGGCTGCTTCACATCTATTTTCGGCTTGCTTGGTAGAGGAGGCAATTGAGCTATTGGTAGCATATATATTTTTGAAGCCTTTTCCATTTAATGCTCCATGCTCACGGATCACTGGATTTTTAAGGTTCCTGCAATTACTATCAGATTATGACTGGACCTTTTCTGCGTTAGTTGTTGACATAAATAATGATTTAACATCAAAGGATGAGAAAGAGATAAGAGAGAACTTCATGTTAAGTAGAAAAGGTTATGAAGAAAATCCTCAAAATGTAAACTCAGCAATGTTCCTAGCTACATCCTATGACACGGCATCTGAGGCTTGGACCAAATTTTCACCAAATTCAATGGAGCTAAAAAGGTTGATGGCTTATGCTGGAAGCAGTGCAAACTTGTTGACCAAACTGATATTGGAGGACCATAGTGATTCTTATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAATTATGATGCTGTAATTCTTCTCCATAGGGAAAAATTACCTTATCCTCAACGCCTTCTCTTTCCATCAGAATTACATCAAGGCGTTCATGTGGCATGTGGGAATGCAAGCAAGGGTTTCCACCCGTTTCTTTTGCCTGGAGATTTTAAAGGAAGCTCAGAGGATCTAAGAAACAAAGTATTGGTAAACTTCGATCCGCTAAGGTGTTTTGTTGGGGATCTAGAGAAAGAGTACCCCAACACTTTCAAGCTATGGTATGATTCTCTGGGTGGTGATGCTGTTGGCATAACATGGGGAGGATGTATTTCAAAGAAACGGGGCCGGGAAGAAGCAGATGAGGAAGTAAGGGATCCAATTGATTTGTTAAAAGATGTTGGTAAAGTTGGAACAGGGTTCGTCAGGGGCATTTATCTTCTAAAAGCCCCAAGGCGGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

1050

Amino Acids

119.38

Weight (kDa)

8.27

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 95 - 227 1.1e-34 Nrap protein domain 1
Nrap_D2 PF17403 234 - 375 2.5e-31 Nrap protein PAP/OAS-like domain
Nrap_D3 PF17404 380 - 534 4.9e-37 Nrap protein domain 3
Nrap_D4 PF17405 556 - 735 5e-44 Nrap protein nucleotidyltransferase domain 4
Nrap_D5 PF17406 737 - 889 5.2e-47 Nrap protein PAP/OAS1-like domain 5
Nrap_D6 PF17407 903 - 1010 1e-11 Nrap protein domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 567
Acc65I GGTACC 1 cut(s) 345
AccB1I GGYRCC 3 cut(s) 345, 360, 1661
AccB7I CCANNNNNTGG 1 cut(s) 2219
AccI GTMKAC 2 cut(s) 90, 608
AccII CGCG 1 cut(s) 74
AccIII TCCGGA 2 cut(s) 447, 1685
AciI CCGC 9 cut(s) 20, 72, 240, 565, 739, 1823, 1886, 2909, 3142
AcoI YGGCCR 1 cut(s) 1883
AcsI RAATTY 8 cut(s) 203, 869, 1374, 1454, 1710, 2073, 2576, 2587
AcuI CTGAAG 4 cut(s) 47, 743, 867, 1976
AcvI CACGTG 1 cut(s) 2128
AcyI GRCGYC 1 cut(s) 96
AfiI CCNNNNNNNGG 6 cut(s) 31, 1780, 1881, 1888, 2219, 3141
AflII CTTAAG 1 cut(s) 1402
AflIII ACRYGT 4 cut(s) 642, 1291, 2012, 2125
AgeI ACCGGT 1 cut(s) 342
AhdI GACNNNNNGTC 1 cut(s) 1556
AjiI CACGTC 1 cut(s) 2108
AjnI CCWGG 4 cut(s) 496, 1121, 1298, 2848
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
Alw21I GWGCWC 2 cut(s) 42, 1388
Alw26I GTCTC 2 cut(s) 2053, 2098
AlwNI CAGNNNCTG 1 cut(s) 2024
Aor13HI TCCGGA 2 cut(s) 447, 1685
AoxI GGCC 7 cut(s) 922, 1770, 1883, 1925, 2089, 2222, 3031
ApeKI GCWGC 4 cut(s) 116, 562, 1045, 2252
ApoI RAATTY 8 cut(s) 203, 869, 1374, 1454, 1710, 2073, 2576, 2587
AsiGI ACCGGT 1 cut(s) 342
Asp700I GAANNNNTTC 4 cut(s) 969, 1323, 1743, 1862
Asp718I GGTACC 1 cut(s) 345
AspLEI GCGC 2 cut(s) 76, 1731
AsuC2I CCSGG 3 cut(s) 168, 1019, 3035
AsuHPI GGTGA 4 cut(s) 60, 70, 2574, 2996
AsuII TTCGAA 1 cut(s) 2698
AsuNHI GCTAGC 1 cut(s) 1825
AvaII GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
BamHI GGATCC 1 cut(s) 3061
BanI GGYRCC 3 cut(s) 345, 360, 1661
BanII GRGCYC 4 cut(s) 42, 736, 906, 1388
BbrPI CACGTG 1 cut(s) 2128
BbsI GAAGAC 5 cut(s) 645, 1289, 1746, 1795, 1982
Bbv12I GWGCWC 2 cut(s) 42, 1388
BbvI GCAGC 4 cut(s) 128, 574, 1032, 2239
BccI CCATC 8 cut(s) 128, 538, 1000, 1496, 2241, 2605, 2676, 2785
BceAI ACGGC 3 cut(s) 83, 168, 2571
BcgI CGANNNNNNTGC 4 cut(s) 836, 870, 1708, 1742
BciT130I CCWGG 4 cut(s) 498, 1123, 1300, 2850
BciVI GTATCC 1 cut(s) 480
BcnI CCSGG 3 cut(s) 168, 1019, 3035
BcoDI GTCTC 2 cut(s) 2053, 2098
BfaI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
BfmI CTRYAG 3 cut(s) 252, 560, 1761
BfrI CTTAAG 1 cut(s) 1402
BfuAI ACCTGC 1 cut(s) 567
BfuI GTATCC 1 cut(s) 480
BisI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
BlpI GCTNAGC 1 cut(s) 1482
BlsI GCNGC 7 cut(s) 118, 242, 564, 740, 1047, 1887, 2254
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2850, 3035
Bme18I GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
BmeRI GACNNNNNGTC 1 cut(s) 1556
BmgBI CACGTC 1 cut(s) 2108
BmiI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
BmrFI CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2850, 3035
BmrI ACTGGG 1 cut(s) 2124
BmsI GCATC 6 cut(s) 735, 1417, 1887, 2567, 2710, 2977
BmtI GCTAGC 1 cut(s) 1829
BmuI ACTGGG 1 cut(s) 2124
BoxI GACNNNNGTC 1 cut(s) 1419
BpiI GAAGAC 5 cut(s) 645, 1289, 1746, 1795, 1982
BpmI CTGGAG 1 cut(s) 2871
Bpu10I CCTNAGC 2 cut(s) 1487, 2912
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 1 cut(s) 2698
BpuEI CTTGAG 2 cut(s) 128, 1061
BpuMI CCSGG 3 cut(s) 168, 1019, 3035
Bsa29I ATCGAT 1 cut(s) 107
BsaAI YACGTR 2 cut(s) 1292, 2128
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 4 cut(s) 306, 496, 497, 3137
BsaWI WCCGGW 3 cut(s) 342, 447, 1685
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 1780, 1881, 1888, 2219, 3141
Bse118I RCCGGY 2 cut(s) 342, 2224
Bse1I ACTGG 7 cut(s) 473, 1074, 1903, 1928, 2119, 2365, 2405
Bse3DI GCAATG 4 cut(s) 849, 873, 1547, 2535
BseAI TCCGGA 2 cut(s) 447, 1685
BseBI CCWGG 4 cut(s) 498, 1123, 1300, 2850
BseCI ATCGAT 1 cut(s) 107
BseDI CCNNGG 4 cut(s) 306, 496, 497, 3137
BseLI CCNNNNNNNGG 6 cut(s) 31, 1780, 1881, 1888, 2219, 3141
BseMI GCAATG 4 cut(s) 849, 873, 1547, 2535
BseNI ACTGG 7 cut(s) 473, 1074, 1903, 1928, 2119, 2365, 2405
BseRI GAGGAG 3 cut(s) 50, 959, 2300
BseX3I CGGCCG 1 cut(s) 1883
BseXI GCAGC 4 cut(s) 128, 574, 1032, 2239
BseYI CCCAGC 1 cut(s) 2212
BsgI GTGCAG 2 cut(s) 210, 1787
Bsh1236I CGCG 1 cut(s) 74
Bsh1285I CGRYCG 2 cut(s) 1886, 3146
BshFI GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
BshNI GGYRCC 3 cut(s) 345, 360, 1661
BshTI ACCGGT 1 cut(s) 342
BshVI ATCGAT 1 cut(s) 107
BsiEI CGRYCG 2 cut(s) 1886, 3146
BsiHKAI GWGCWC 2 cut(s) 42, 1388
BsiSI CCGG 8 cut(s) 167, 343, 448, 1018, 1686, 1882, 2225, 3034
BslI CCNNNNNNNGG 6 cut(s) 31, 1780, 1881, 1888, 2219, 3141
BsmAI GTCTC 2 cut(s) 2053, 2098
BsmBI CGTCTC 1 cut(s) 2098
BsmI GAATGC 4 cut(s) 543, 597, 1964, 2821
BsnI GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
Bsp119I TTCGAA 1 cut(s) 2698
Bsp1286I GDGCHC 4 cut(s) 42, 736, 906, 1388
Bsp13I TCCGGA 2 cut(s) 447, 1685
Bsp1407I TGTACA 1 cut(s) 2015
Bsp1720I GCTNAGC 1 cut(s) 1482
BspACI CCGC 9 cut(s) 20, 72, 240, 565, 739, 1823, 1886, 2909, 3142
BspANI GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
BspDI ATCGAT 1 cut(s) 107
BspEI TCCGGA 2 cut(s) 447, 1685
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
BspMAI CTGCAG 1 cut(s) 564
BspMI ACCTGC 1 cut(s) 567
BspOI GCTAGC 1 cut(s) 1829
BspT104I TTCGAA 1 cut(s) 2698
BspT107I GGYRCC 3 cut(s) 345, 360, 1661
BspTI CTTAAG 1 cut(s) 1402
BsrDI GCAATG 4 cut(s) 849, 873, 1547, 2535
BsrFI RCCGGY 2 cut(s) 342, 2224
BsrGI TGTACA 1 cut(s) 2015
BsrI ACTGG 7 cut(s) 473, 1074, 1903, 1928, 2119, 2365, 2405
BssAI RCCGGY 2 cut(s) 342, 2224
BssECI CCNNGG 4 cut(s) 306, 496, 497, 3137
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 306, 3137
Bst2UI CCWGG 4 cut(s) 498, 1123, 1300, 2850
Bst4CI ACNGT 3 cut(s) 1288, 1525, 1765
Bst6I CTCTTC 1 cut(s) 1172
BstACI GRCGYC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 1402
BstAUI TGTACA 1 cut(s) 2015
BstBAI YACGTR 2 cut(s) 1292, 2128
BstBI TTCGAA 1 cut(s) 2698
BstC8I GCNNGC 7 cut(s) 736, 1075, 1770, 1827, 2238, 2275, 2821
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 2 cut(s) 76, 1731
BstMAI GTCTC 2 cut(s) 2053, 2098
BstMCI CGRYCG 2 cut(s) 1886, 3146
BstMWI GCNNNNNNNGC 2 cut(s) 161, 2564
BstNI CCWGG 4 cut(s) 498, 1123, 1300, 2850
BstNSI RCATGY 5 cut(s) 646, 1077, 1583, 2016, 2811
BstPAI GACNNNNGTC 1 cut(s) 1419
BstSCI CCNGG 7 cut(s) 166, 496, 1017, 1121, 1298, 2848, 3033
BstSFI CTRYAG 3 cut(s) 252, 560, 1761
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 4 cut(s) 128, 574, 1032, 2239
BstV2I GAAGAC 5 cut(s) 645, 1289, 1746, 1795, 1982
BstX2I RGATCY 5 cut(s) 286, 1669, 2875, 2929, 3061
BstYI RGATCY 5 cut(s) 286, 1669, 2875, 2929, 3061
BstZI CGGCCG 1 cut(s) 1883
Bsu15I ATCGAT 1 cut(s) 107
BsuI GTATCC 1 cut(s) 480
BsuRI GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
BsuTUI ATCGAT 1 cut(s) 107
BtrI CACGTC 1 cut(s) 2108
BtsI GCAGTG 2 cut(s) 1361, 2635
BtsIMutI CAGTG 7 cut(s) 1067, 1361, 1386, 1770, 2022, 2358, 2635
BveI ACCTGC 1 cut(s) 567
Cac8I GCNNGC 7 cut(s) 736, 1075, 1770, 1827, 2238, 2275, 2821
CaiI CAGNNNCTG 1 cut(s) 2024
CfoI GCGC 2 cut(s) 76, 1731
Cfr10I RCCGGY 2 cut(s) 342, 2224
ClaI ATCGAT 1 cut(s) 107
CseI GACGC 1 cut(s) 104
CspAI ACCGGT 1 cut(s) 342
DraI TTTAAA 2 cut(s) 961, 2860
DriI GACNNNNNGTC 1 cut(s) 1556
EaeI YGGCCR 1 cut(s) 1883
EagI CGGCCG 1 cut(s) 1883
Eam1104I CTCTTC 1 cut(s) 1172
Eam1105I GACNNNNNGTC 1 cut(s) 1556
EarI CTCTTC 1 cut(s) 1172
Ecl136II GAGCTC 2 cut(s) 40, 1386
EclXI CGGCCG 1 cut(s) 1883
Eco130I CCWWGG 2 cut(s) 306, 3137
Eco147I AGGCCT 1 cut(s) 2091
Eco24I GRGCYC 4 cut(s) 42, 736, 906, 1388
Eco32I GATATC 1 cut(s) 1572
Eco47I GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
Eco52I CGGCCG 1 cut(s) 1883
Eco53kI GAGCTC 2 cut(s) 40, 1386
Eco57I CTGAAG 4 cut(s) 47, 743, 867, 1976
Eco72I CACGTG 1 cut(s) 2128
EcoICRI GAGCTC 2 cut(s) 40, 1386
EcoO109I RGGNCCY 1 cut(s) 1771
EcoRI GAATTC 1 cut(s) 203
EcoRII CCWGG 4 cut(s) 496, 1121, 1298, 2848
EcoRV GATATC 1 cut(s) 1572
EcoT14I CCWWGG 2 cut(s) 306, 3137
EcoT22I ATGCAT 2 cut(s) 1079, 1626
EcoT38I GRGCYC 4 cut(s) 42, 736, 906, 1388
ErhI CCWWGG 2 cut(s) 306, 3137
Esp3I CGTCTC 1 cut(s) 2098
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FauI CCCGC 1 cut(s) 79
FauNDI CATATG 1 cut(s) 2218
FblI GTMKAC 2 cut(s) 90, 608
Fnu4HI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
FriOI GRGCYC 4 cut(s) 42, 736, 906, 1388
Fsp4HI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
FspBI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
GlaI GCGC 2 cut(s) 75, 1730
GluI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
GsaI CCCAGC 1 cut(s) 2216
GsuI CTGGAG 1 cut(s) 2871
HaeIII GGCC 7 cut(s) 924, 1772, 1885, 1927, 2091, 2224, 3033
HapII CCGG 8 cut(s) 167, 343, 448, 1018, 1686, 1882, 2225, 3034
HgaI GACGC 1 cut(s) 104
HhaI GCGC 2 cut(s) 76, 1731
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 2 cut(s) 74, 1729
HinP1I GCGC 2 cut(s) 74, 1729
HincII GTYRAC 4 cut(s) 1414, 2174, 2425, 2643
HindII GTYRAC 4 cut(s) 1414, 2174, 2425, 2643
HindIII AAGCTT 1 cut(s) 1000
HpaII CCGG 8 cut(s) 167, 343, 448, 1018, 1686, 1882, 2225, 3034
HphI GGTGA 4 cut(s) 60, 70, 2574, 2996
Hpy99I CGWCG 3 cut(s) 98, 403, 406
HpyCH4III ACNGT 3 cut(s) 1288, 1525, 1765
HpyCH4IV ACGT 3 cut(s) 1291, 2107, 2127
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 2564
HpySE526I ACGT 3 cut(s) 1291, 2107, 2127
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 2 cut(s) 74, 1729
Kpn2I TCCGGA 2 cut(s) 447, 1685
KpnI GGTACC 1 cut(s) 349
LmnI GCTCC 4 cut(s) 37, 169, 2347, 2596
Lsp1109I GCAGC 4 cut(s) 128, 574, 1032, 2239
LweI GCATC 6 cut(s) 735, 1417, 1887, 2567, 2710, 2977
MaeI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
MaeII ACGT 3 cut(s) 1291, 2107, 2127
MaeIII GTNAC 8 cut(s) 148, 475, 859, 1021, 1271, 1294, 1344, 2207
MfeI CAATTG 3 cut(s) 975, 2292, 3066
MflI RGATCY 5 cut(s) 286, 1669, 2875, 2929, 3061
MhlI GDGCHC 4 cut(s) 42, 736, 906, 1388
MlyI GAGTC 1 cut(s) 1996
MmeI TCCRAC 4 cut(s) 1012, 1202, 1887, 3076
Mph1103I ATGCAT 2 cut(s) 1079, 1626
MroI TCCGGA 2 cut(s) 447, 1685
MroXI GAANNNNTTC 4 cut(s) 969, 1323, 1743, 1862
MslI CAYNNNNRTG 1 cut(s) 1072
MspA1I CMGCKG 3 cut(s) 20, 565, 1888
MspCI CTTAAG 1 cut(s) 1402
MspI CCGG 8 cut(s) 167, 343, 448, 1018, 1686, 1882, 2225, 3034
MspR9I CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2850, 3035
MunI CAATTG 3 cut(s) 975, 2292, 3066
Mva1269I GAATGC 4 cut(s) 543, 597, 1964, 2821
MvaI CCWGG 4 cut(s) 498, 1123, 1300, 2850
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 2 cut(s) 161, 2564
NciI CCSGG 3 cut(s) 168, 1019, 3035
NdeI CATATG 1 cut(s) 2218
NheI GCTAGC 1 cut(s) 1825
NlaIV GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
NmuCI GTSAC 4 cut(s) 475, 1271, 1294, 1344
NsiI ATGCAT 2 cut(s) 1079, 1626
NspI RCATGY 5 cut(s) 646, 1077, 1583, 2016, 2811
NspV TTCGAA 1 cut(s) 2698
PaeI GCATGC 1 cut(s) 1077
PasI CCCWGGG 1 cut(s) 497
PceI AGGCCT 1 cut(s) 2091
PciI ACATGT 2 cut(s) 642, 2012
PctI GAATGC 4 cut(s) 543, 597, 1964, 2821
PdmI GAANNNNTTC 4 cut(s) 969, 1323, 1743, 1862
PflFI GACNNNGTC 1 cut(s) 1151
PflMI CCANNNNNTGG 1 cut(s) 2219
PfoI TCCNGGA 1 cut(s) 166
PinAI ACCGGT 1 cut(s) 342
PkrI GCNGC 7 cut(s) 118, 242, 564, 740, 1047, 1887, 2254
PleI GAGTC 1 cut(s) 1995
PmaCI CACGTG 1 cut(s) 2128
PmlI CACGTG 1 cut(s) 2128
PpsI GAGTC 1 cut(s) 1995
Ppu21I YACGTR 2 cut(s) 1292, 2128
PscI ACATGT 2 cut(s) 642, 2012
PshAI GACNNNNGTC 1 cut(s) 1419
Psp124BI GAGCTC 2 cut(s) 42, 1388
Psp6I CCWGG 4 cut(s) 496, 1121, 1298, 2848
PspCI CACGTG 1 cut(s) 2128
PspFI CCCAGC 1 cut(s) 2212
PspGI CCWGG 4 cut(s) 496, 1121, 1298, 2848
PspN4I GGNNCC 7 cut(s) 347, 362, 818, 1663, 2375, 3032, 3063
PstI CTGCAG 1 cut(s) 564
PstNI CAGNNNCTG 1 cut(s) 2024
PsuI RGATCY 5 cut(s) 286, 1669, 2875, 2929, 3061
PsyI GACNNNGTC 1 cut(s) 1151
RseI CAYNNNNRTG 1 cut(s) 1072
SacI GAGCTC 2 cut(s) 42, 1388
SatI GCNGC 7 cut(s) 117, 241, 563, 739, 1046, 1886, 2253
ScaI AGTACT 1 cut(s) 389
SchI GAGTC 1 cut(s) 1996
ScrFI CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2850, 3035
SduI GDGCHC 4 cut(s) 42, 736, 906, 1388
SfaNI GCATC 6 cut(s) 735, 1417, 1887, 2567, 2710, 2977
SfcI CTRYAG 3 cut(s) 252, 560, 1761
SfuI TTCGAA 1 cut(s) 2698
SinI GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
SmiMI CAYNNNNRTG 1 cut(s) 1072
SmlI CTYRAG 3 cut(s) 143, 1040, 1402
SmoI CTYRAG 3 cut(s) 143, 1040, 1402
SphI GCATGC 1 cut(s) 1077
SseBI AGGCCT 1 cut(s) 2091
SsiI CCGC 9 cut(s) 20, 72, 240, 565, 739, 1823, 1886, 2909, 3142
SspI AATATT 2 cut(s) 65, 852
SspMI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
SstI GAGCTC 2 cut(s) 42, 1388
StuI AGGCCT 1 cut(s) 2091
StyD4I CCNGG 7 cut(s) 166, 496, 1017, 1121, 1298, 2848, 3033
StyI CCWWGG 2 cut(s) 306, 3137
TaaI ACNGT 3 cut(s) 1288, 1525, 1765
TaiI ACGT 3 cut(s) 1294, 2110, 2130
TaqI TCGA 5 cut(s) 107, 207, 1718, 2698, 2904
TaqII GACCGA 1 cut(s) 1290
TatI WGTACW 4 cut(s) 387, 618, 986, 2015
TauI GCSGC 3 cut(s) 243, 741, 1888
TscAI CASTG 7 cut(s) 1074, 1368, 1386, 1770, 2029, 2365, 2635
TseFI GTSAC 4 cut(s) 475, 1271, 1294, 1344
TseI GCWGC 4 cut(s) 116, 562, 1045, 2252
Tsp45I GTSAC 4 cut(s) 475, 1271, 1294, 1344
TspGWI ACGGA 2 cut(s) 1448, 2368
TspRI CASTG 7 cut(s) 1074, 1368, 1386, 1770, 2029, 2365, 2635
Tth111I GACNNNGTC 1 cut(s) 1151
Van91I CCANNNNNTGG 1 cut(s) 2219
Vha464I CTTAAG 1 cut(s) 1402
VpaK11BI GGWCC 7 cut(s) 22, 52, 426, 1421, 2403, 2571, 2662
XapI RAATTY 8 cut(s) 203, 869, 1374, 1454, 1710, 2073, 2576, 2587
XbaI TCTAGA 2 cut(s) 995, 2932
XceI RCATGY 5 cut(s) 646, 1077, 1583, 2016, 2811
XcmI CCANNNNNNNNNTGG 1 cut(s) 2653
XmiI GTMKAC 2 cut(s) 90, 608
XmnI GAANNNNTTC 4 cut(s) 969, 1323, 1743, 1862
XspI CTAG 6 cut(s) 59, 996, 1775, 1826, 2537, 2933
ZrmI AGTACT 1 cut(s) 389
Zsp2I ATGCAT 2 cut(s) 1079, 1626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.