RchiOBHm_Chr2g0101431

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008672
Physical Location & Seq
Forward (+)
13072995 .. 13081422
8428 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47603

Sequence Viewer

Length: 3153 bp
ATGGAAACCGATAACTCAGCGGACCTGAAGCTGGAGGAGCTCTTGAAAGAGGTCCAGCTAGAATATTCACCTGCTCTCACCAAGTTCGTAGACGACGCCGTTTCATCGATCAAAGCAGCCATCGATACAATTCCAAAAGACCTCAAGGTTACGGCTGATTTAGCTCCGGGATTCGTCAGAGATATTGGTGCAGACAAAGTTGAATTTGATTTCAAGAAGCCGAAATCTGTCAAAATTGGCGGCAGTTATGCTATAGAGTGCCTTGTAAAGCCTGAGTTCAATGTGGATCTTTTCGTTCAGTTGCCCAAGGAATGTTTCCATGAGAAAGACTATTTGAATTACCGGTACCATGCTAAAAGGTGCCTGTACCTTTGTGTGATTAAAAAGTACTTGATGTCGTCTTCGTTAGTTGGTAAGGTTGAATGGTCCACTTTCCAGAACGAAGTCCGGAAACCTGTGTTGATTGTGTATCCAGTCACGAAATTAGTTGCACTCCCTGGGTTCTTTGTGAGAATAATTCCCGCAGCACCATCTCTTTTTAGCATTCCAAAATTGAACCTGCAGCGGAATAATGTTCGTGCTGTGAGTACAGGGGGCATTCCTCAGTCTACTCCCAAGTACAATAGTAGTATTTTGGAAGACATGTTTATAGAAGATACAGAAAAACTTGTCAAGCAAACTTTTCTTGGGTCAAAGGAATTACGAGAAGGTTTAGTTTTGCTGAAGGTCTGGGCTCGCCGCAGGATACCAATATATGCTTATGATTGCCTGAATGGTTTTTTAATCTCTGTCATATTGGCATATCTTGTGGATAGGAACCATATCAATAAATCAATGAAGGCAATGCAAATATTTCGTGTTACAATGAAATTCATTGCTACTTCAGATTTGTGGAAACATGGGCTCTACTTTACACCAAAAGGCCAAAAAGCTATTTCAAAAGAGGAGAGGTTGCCTTTTAAAGAATCATTTCCAACTGTTATATGTACTCCATCTAGAAGCTTTAATTTAGCCTTCCGGGTAACAAGGGTTGGATTTCTCGAGCTGCAAAATGAATCTGCAATGACACTGGCATGCATAGAGAAATGCAGAGACAGTGGATTTGAGGAAGTATTTATGACCAGGATTGACTATCCTGTAAAATATGACCATGTCTTAAGGTTGAACTTAAAGGGGAAGAGTTCAGTTTATGGATTGGGATTTTGCTTGGATGATGAATGTTGGAGAGTGTATGAGCAGAAGGTTCATAATGTTTTAAGTCATGGGTTAAGTGACCGAGTGAAGACTGTACGTGTGACCTGGAAAAGCATGTTATCAGAATCCATTCTACAGAATGGATTGTCGTCACTCAATACAGAGCCACTGCTAATTGGAATTTCAGTGAGCTCTTTAGATAAAGTTTTAAGAATTGTTGATATTGGTCCTGATGCTGATAATAAGGATGAGGCTCTCAAATTCCGTCAGTTCTGGGGGGATAAGGCTGAGCTTAGGAGATTCAAAGATGGCAAGATAGCAGAAAGCACAGTTTGGGAGACTGAGCAATGGAAAAGACATATCATCATGAAAGAGATATCTGAGCATGTGCTTCTGCGACATCTTTCACTATCAAAAGAGAATATCATGCATATCGTGGATCAACTTGATTTCTCTTTACAATATGGTGCCGAAGATCCTATATCTTCTTCTGGGAGTTTGTTTGGGGCATTTGAAATTCTATCGAAGCGATTGCGCCTTATTGAAGACATTCCTCTAAAGGTTTCTACAGTGCAGGCCCTAGATTCGGCTTTCAGATATTCATCTGTCTTCCCACCTGAACCTCATCCGCTAGCTAATGAAAAGGGTTCTTTCGTAAGACTAAAACTAAATCAGTTACCTCCTTCCTGCATCCGGCCGCTGGAAGTTATGATCCAGTTGGAAGGTTCTGGAAACTGGCCTATGGATGATATAGCAATTGAGAAAACTAAATCAGCATTCCTTCTTCAAATCGGAGAGAGTCTTCAGAATAGTTGGGGGATGACATGTACAGCCACTGAGGATGATGTGGACGTTTTTGTGTCTGGATATGCCTTTCGTCTTAAAATTTGGCATGAGAGAGGCCTGACTTTGTTGAGACGAGAAACTGGGAATGAACACGTGAAGAAGGTTTCTAATGTGGACAAGGAACTATACTTTCGCAGTCAACATTCCAGCATGATAAATGGATTCCAGACTTGTTTCCCAGCATATGGGCCAGTTGTTAGGCTTGCAAAACGATGGGCTGCTTCACATCTATTTTCGGCTTGCTTGGTAGAGGAGGCAATTGAGCTATTGGTAGCATATATATTTTTGAAGCCTTTACCATTTAATGCTCCATGCTCACGGGTCACTGGATTTTTAAGGTTCCTGAGATTACTATCAGATTATGACTGGACCTTTTCTGCATTAGTTGTTGACATAAATAATGATTTAACATCAAATGATGAGAAAGAGATAAGAGAGAACTTCATGTTAAGTAGAAAAGGTTATGAAGAAAATCCTCAAAATGTAAACTCAGCAATGTTCCTAGCTACATCCTACGACAAGGCATCTGAGGCTTGGACCAAATTTTCACCAAACTCAATGCTAAAAAGGTTGATGGCTTATGCTGGAAGCAGTGCAAACTTGTTGACCAAACTGATATTGGAGGACCATAGTGATTCTTATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAATTATGATGCTGTAATTCTTCTCCATAGAGAAAAATTACCTTATCCCCAACGCCTTCTCTTTCCATCAGAATTACATCAAGGGGTTCATGTGGCACGTGGGAATGCGAGCAAGGGTTTCCACCCCTTTCTTTTGCCTGGAGATTTTAAAGGAAGCTCAGAGGAACTAAGAAACAAAGTATTGGTGAACTTCGATCCGCTGAGGTGTTTTGTTGGGGATCTAGAGAAAGAGTACTCAAACACTTTCAAGCTATGGTATGATTCTCTGGGTGGTGATGCTGTTGGTATAACATGGAGAGGATGTATTTCAAAGAAACGGGGCCGGGAAGAAGCAGATGAGGAAGTAAAGGATCCAATTGATTTGTTAAAAGATGTTGGTAAAGTTGGAACAGGGTTCGTCAGGGGCATTTATCTTCTAAAAGCCCCAAGGCGGTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

1050

Amino Acids

119.7

Weight (kDa)

8.52

Isoelectric Point (pI)

42.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 95 - 227 3.6e-35 Nrap protein domain 1
Nrap_D2 PF17403 234 - 375 4.2e-31 Nrap protein PAP/OAS-like domain
Nrap_D3 PF17404 380 - 534 3.8e-37 Nrap protein domain 3
Nrap_D4 PF17405 555 - 737 1e-44 Nrap protein nucleotidyltransferase domain 4
Nrap_D5 PF17406 739 - 890 5e-46 Nrap protein PAP/OAS1-like domain 5
Nrap_D6 PF17407 904 - 1011 8.5e-13 Nrap protein domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 79
Acc36I ACCTGC 2 cut(s) 79, 567
Acc65I GGTACC 1 cut(s) 345
AccB1I GGYRCC 3 cut(s) 345, 360, 1661
AccB7I CCANNNNNTGG 1 cut(s) 2225
AccI GTMKAC 2 cut(s) 90, 608
AccIII TCCGGA 1 cut(s) 447
AciI CCGC 9 cut(s) 20, 240, 522, 565, 739, 1823, 1892, 2912, 3145
AclWI GGATC 8 cut(s) 294, 1641, 1664, 1900, 2903, 2940, 3059, 3072
AcoI YGGCCR 1 cut(s) 1889
AcsI RAATTY 7 cut(s) 203, 869, 1374, 1454, 1710, 2079, 2582
AcuI CTGAAG 4 cut(s) 47, 743, 867, 1982
AcvI CACGTG 2 cut(s) 2134, 2813
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 9 cut(s) 347, 368, 389, 589, 620, 988, 1290, 2023, 2948
AfiI CCNNNNNNNGG 6 cut(s) 31, 1780, 1887, 1894, 2225, 3144
AflII CTTAAG 1 cut(s) 1156
AflIII ACRYGT 4 cut(s) 642, 1291, 2018, 2131
AgeI ACCGGT 1 cut(s) 342
AjnI CCWGG 4 cut(s) 496, 1121, 1298, 2851
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
Alw21I GWGCWC 2 cut(s) 42, 1388
Alw26I GTCTC 3 cut(s) 1086, 1526, 2104
AlwI GGATC 8 cut(s) 294, 1641, 1664, 1900, 2903, 2940, 3059, 3072
AlwNI CAGNNNCTG 1 cut(s) 2030
Ama87I CYCGRG 1 cut(s) 1040
Aor13HI TCCGGA 1 cut(s) 447
AoxI GGCC 7 cut(s) 922, 1770, 1889, 1931, 2095, 2228, 3034
ApeKI GCWGC 5 cut(s) 116, 524, 562, 1045, 2258
ApoI RAATTY 7 cut(s) 203, 869, 1374, 1454, 1710, 2079, 2582
ArsI GACNNNNNNTTYG 2 cut(s) 1085, 1117
AsiGI ACCGGT 1 cut(s) 342
Asp700I GAANNNNTTC 3 cut(s) 969, 1323, 1743
Asp718I GGTACC 1 cut(s) 345
AspLEI GCGC 1 cut(s) 1731
AsuC2I CCSGG 3 cut(s) 168, 1019, 3038
AsuHPI GGTGA 5 cut(s) 60, 70, 2580, 2911, 2999
AsuII TTCGAA 1 cut(s) 2701
AsuNHI GCTAGC 1 cut(s) 1825
AvaI CYCGRG 1 cut(s) 1040
AvaII GGWCC 7 cut(s) 22, 52, 426, 1421, 2409, 2577, 2665
BamHI GGATCC 1 cut(s) 3064
BanI GGYRCC 3 cut(s) 345, 360, 1661
BanII GRGCYC 4 cut(s) 42, 736, 906, 1388
BbrPI CACGTG 2 cut(s) 2134, 2813
BbsI GAAGAC 6 cut(s) 393, 645, 1289, 1746, 1795, 1988
Bbv12I GWGCWC 2 cut(s) 42, 1388
BbvCI CCTCAGC 1 cut(s) 2915
BbvI GCAGC 5 cut(s) 128, 536, 574, 1032, 2245
BccI CCATC 8 cut(s) 128, 538, 1000, 1496, 2247, 2608, 2679, 2788
BceAI ACGGC 2 cut(s) 83, 168
BcgI CGANNNNNNTGC 4 cut(s) 836, 870, 1708, 1742
BciT130I CCWGG 4 cut(s) 498, 1123, 1300, 2853
BciVI GTATCC 2 cut(s) 480, 738
BcnI CCSGG 3 cut(s) 168, 1019, 3038
BcoDI GTCTC 3 cut(s) 1086, 1526, 2104
BfaI CTAG 6 cut(s) 59, 996, 1775, 1826, 2543, 2936
BfmI CTRYAG 4 cut(s) 252, 560, 1328, 1761
BfrI CTTAAG 1 cut(s) 1156
BfuAI ACCTGC 2 cut(s) 79, 567
BfuI GTATCC 2 cut(s) 480, 738
BisI GCNGC 8 cut(s) 117, 241, 525, 563, 739, 1046, 1892, 2259
BlpI GCTNAGC 1 cut(s) 1482
BlsI GCNGC 8 cut(s) 118, 242, 526, 564, 740, 1047, 1893, 2260
BmcAI AGTACT 2 cut(s) 389, 2948
Bme1390I CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2853, 3038
Bme18I GGWCC 7 cut(s) 22, 52, 426, 1421, 2409, 2577, 2665
BmeT110I CYCGRG 1 cut(s) 1040
BmiI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2381, 3035, 3066
BmrFI CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2853, 3038
BmrI ACTGGG 1 cut(s) 2130
BmsI GCATC 5 cut(s) 1417, 1893, 2573, 2713, 2980
BmtI GCTAGC 1 cut(s) 1829
BmuI ACTGGG 1 cut(s) 2130
BpiI GAAGAC 6 cut(s) 393, 645, 1289, 1746, 1795, 1988
BpmI CTGGAG 2 cut(s) 53, 2874
Bpu10I CCTNAGC 2 cut(s) 1487, 2915
Bpu1102I GCTNAGC 1 cut(s) 1482
Bpu14I TTCGAA 1 cut(s) 2701
BpuEI CTTGAG 1 cut(s) 128
BpuMI CCSGG 3 cut(s) 168, 1019, 3038
Bsa29I ATCGAT 2 cut(s) 107, 123
BsaAI YACGTR 3 cut(s) 1292, 2134, 2813
BsaBI GATNNNNATC 2 cut(s) 1795, 2392
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 4 cut(s) 306, 496, 497, 3140
BsaWI WCCGGW 2 cut(s) 342, 447
BsaXI ACNNNNNCTCC 2 cut(s) 3001, 3031
Bsc4I CCNNNNNNNGG 6 cut(s) 31, 1780, 1887, 1894, 2225, 3144
Bse118I RCCGGY 1 cut(s) 342
Bse1I ACTGG 8 cut(s) 473, 1074, 1909, 1934, 2125, 2231, 2371, 2411
Bse3DI GCAATG 5 cut(s) 849, 873, 1068, 1547, 2541
Bse8I GATNNNNATC 2 cut(s) 1795, 2392
BseAI TCCGGA 1 cut(s) 447
BseBI CCWGG 4 cut(s) 498, 1123, 1300, 2853
BseCI ATCGAT 2 cut(s) 107, 123
BseDI CCNNGG 4 cut(s) 306, 496, 497, 3140
BseGI GGATG 9 cut(s) 1216, 1447, 1819, 1884, 1945, 2019, 2041, 2549, 3020
BseJI GATNNNNATC 2 cut(s) 1795, 2392
BseLI CCNNNNNNNGG 6 cut(s) 31, 1780, 1887, 1894, 2225, 3144
BseMI GCAATG 5 cut(s) 849, 873, 1068, 1547, 2541
BseNI ACTGG 8 cut(s) 473, 1074, 1909, 1934, 2125, 2231, 2371, 2411
BseRI GAGGAG 3 cut(s) 50, 959, 2306
BseX3I CGGCCG 1 cut(s) 1889
BseXI GCAGC 5 cut(s) 128, 536, 574, 1032, 2245
BseYI CCCAGC 1 cut(s) 2218
BsgI GTGCAG 2 cut(s) 210, 1787
Bsh1285I CGRYCG 2 cut(s) 1892, 3149
BshFI GGCC 7 cut(s) 924, 1772, 1891, 1933, 2097, 2230, 3036
BshNI GGYRCC 3 cut(s) 345, 360, 1661
BshTI ACCGGT 1 cut(s) 342
BshVI ATCGAT 2 cut(s) 107, 123
BsiEI CGRYCG 2 cut(s) 1892, 3149
BsiHKAI GWGCWC 2 cut(s) 42, 1388
BsiHKCI CYCGRG 1 cut(s) 1040
BsiSI CCGG 6 cut(s) 167, 343, 448, 1018, 1888, 3037
BslI CCNNNNNNNGG 6 cut(s) 31, 1780, 1887, 1894, 2225, 3144
BsmAI GTCTC 3 cut(s) 1086, 1526, 2104
BsmBI CGTCTC 1 cut(s) 2104
BsmI GAATGC 4 cut(s) 543, 597, 1970, 2824
BsnI GGCC 7 cut(s) 924, 1772, 1891, 1933, 2097, 2230, 3036
BsoBI CYCGRG 1 cut(s) 1040
Bsp119I TTCGAA 1 cut(s) 2701
Bsp1286I GDGCHC 4 cut(s) 42, 736, 906, 1388
Bsp13I TCCGGA 1 cut(s) 447
Bsp1407I TGTACA 1 cut(s) 2021
Bsp143I GATC 8 cut(s) 108, 286, 1633, 1669, 1905, 2908, 2932, 3064
Bsp1720I GCTNAGC 1 cut(s) 1482
BspACI CCGC 9 cut(s) 20, 240, 522, 565, 739, 1823, 1892, 2912, 3145
BspANI GGCC 7 cut(s) 924, 1772, 1891, 1933, 2097, 2230, 3036
BspDI ATCGAT 2 cut(s) 107, 123
BspEI TCCGGA 1 cut(s) 447
BspHI TCATGA 1 cut(s) 1560
BspLI GGNNCC 7 cut(s) 347, 362, 818, 1663, 2381, 3035, 3066
BspMAI CTGCAG 1 cut(s) 564
BspMI ACCTGC 2 cut(s) 79, 567
BspOI GCTAGC 1 cut(s) 1829
BspPI GGATC 8 cut(s) 294, 1641, 1664, 1900, 2903, 2940, 3059, 3072
BspT104I TTCGAA 1 cut(s) 2701
BspT107I GGYRCC 3 cut(s) 345, 360, 1661
BspTI CTTAAG 1 cut(s) 1156
BsrDI GCAATG 5 cut(s) 849, 873, 1068, 1547, 2541
BsrFI RCCGGY 1 cut(s) 342
BsrGI TGTACA 1 cut(s) 2021
BsrI ACTGG 8 cut(s) 473, 1074, 1909, 1934, 2125, 2231, 2371, 2411
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 4 cut(s) 306, 496, 497, 3140
BssMI GATC 8 cut(s) 108, 286, 1633, 1669, 1905, 2908, 2932, 3064
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 306, 3140
Bst2UI CCWGG 4 cut(s) 498, 1123, 1300, 2853
Bst4CI ACNGT 5 cut(s) 979, 1097, 1288, 1525, 1765
Bst6I CTCTTC 1 cut(s) 1172
BstACI GRCGYC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 1156
BstAUI TGTACA 1 cut(s) 2021
BstBAI YACGTR 3 cut(s) 1292, 2134, 2813
BstBI TTCGAA 1 cut(s) 2701
BstC8I GCNNGC 7 cut(s) 736, 1075, 1770, 1827, 2244, 2281, 2824
BstF5I GGATG 9 cut(s) 1216, 1447, 1819, 1884, 1945, 2019, 2041, 2549, 3020
BstHHI GCGC 1 cut(s) 1731
BstKTI GATC 8 cut(s) 111, 289, 1636, 1672, 1908, 2911, 2935, 3067
BstMAI GTCTC 3 cut(s) 1086, 1526, 2104
BstMBI GATC 8 cut(s) 108, 286, 1633, 1669, 1905, 2908, 2932, 3064
BstMCI CGRYCG 2 cut(s) 1892, 3149
BstMWI GCNNNNNNNGC 3 cut(s) 37, 161, 2570
BstNI CCWGG 4 cut(s) 498, 1123, 1300, 2853
BstNSI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2022
BstSCI CCNGG 7 cut(s) 166, 496, 1017, 1121, 1298, 2851, 3036
BstSFI CTRYAG 4 cut(s) 252, 560, 1328, 1761
BstV1I GCAGC 5 cut(s) 128, 536, 574, 1032, 2245
BstV2I GAAGAC 6 cut(s) 393, 645, 1289, 1746, 1795, 1988
BstX2I RGATCY 4 cut(s) 286, 1669, 2932, 3064
BstYI RGATCY 4 cut(s) 286, 1669, 2932, 3064
BstZI CGGCCG 1 cut(s) 1889
Bsu15I ATCGAT 2 cut(s) 107, 123
BsuI GTATCC 2 cut(s) 480, 738
BsuRI GGCC 7 cut(s) 924, 1772, 1891, 1933, 2097, 2230, 3036
BsuTUI ATCGAT 2 cut(s) 107, 123
BtsCI GGATG 9 cut(s) 1216, 1447, 1819, 1884, 1945, 2019, 2041, 2549, 3020
BtsI GCAGTG 2 cut(s) 1361, 2638
BtsIMutI CAGTG 8 cut(s) 1067, 1102, 1361, 1386, 1770, 2028, 2364, 2638
BveI ACCTGC 2 cut(s) 79, 567
Cac8I GCNNGC 7 cut(s) 736, 1075, 1770, 1827, 2244, 2281, 2824
CaiI CAGNNNCTG 1 cut(s) 2030
CciI TCATGA 1 cut(s) 1560
CfoI GCGC 1 cut(s) 1731
Cfr10I RCCGGY 1 cut(s) 342
ClaI ATCGAT 2 cut(s) 107, 123
CseI GACGC 1 cut(s) 104
Csp6I GTAC 9 cut(s) 346, 367, 388, 588, 619, 987, 1289, 2022, 2947
CspAI ACCGGT 1 cut(s) 342
CviQI GTAC 9 cut(s) 346, 367, 388, 588, 619, 987, 1289, 2022, 2947
DpnI GATC 8 cut(s) 110, 288, 1635, 1671, 1907, 2910, 2934, 3066
DpnII GATC 8 cut(s) 108, 286, 1633, 1669, 1905, 2908, 2932, 3064
DraI TTTAAA 2 cut(s) 961, 2863
EaeI YGGCCR 1 cut(s) 1889
EagI CGGCCG 1 cut(s) 1889
Eam1104I CTCTTC 1 cut(s) 1172
EarI CTCTTC 1 cut(s) 1172
Ecl136II GAGCTC 2 cut(s) 40, 1386
EclXI CGGCCG 1 cut(s) 1889
Eco130I CCWWGG 2 cut(s) 306, 3140
Eco147I AGGCCT 1 cut(s) 2097
Eco24I GRGCYC 4 cut(s) 42, 736, 906, 1388
Eco32I GATATC 1 cut(s) 1572
Eco47I GGWCC 7 cut(s) 22, 52, 426, 1421, 2409, 2577, 2665
Eco52I CGGCCG 1 cut(s) 1889
Eco53kI GAGCTC 2 cut(s) 40, 1386
Eco57I CTGAAG 4 cut(s) 47, 743, 867, 1982
Eco72I CACGTG 2 cut(s) 2134, 2813
Eco88I CYCGRG 1 cut(s) 1040
EcoICRI GAGCTC 2 cut(s) 40, 1386
EcoO109I RGGNCCY 1 cut(s) 1771
EcoRII CCWGG 4 cut(s) 496, 1121, 1298, 2851
EcoRV GATATC 1 cut(s) 1572
EcoT14I CCWWGG 2 cut(s) 306, 3140
EcoT22I ATGCAT 2 cut(s) 1079, 1626
EcoT38I GRGCYC 4 cut(s) 42, 736, 906, 1388
ErhI CCWWGG 2 cut(s) 306, 3140
Esp3I CGTCTC 1 cut(s) 2104
FauI CCCGC 1 cut(s) 529
FauNDI CATATG 1 cut(s) 2224
FblI GTMKAC 2 cut(s) 90, 608
Fnu4HI GCNGC 8 cut(s) 117, 241, 525, 563, 739, 1046, 1892, 2259
FokI GGATG 9 cut(s) 1223, 1454, 1806, 1871, 1952, 2026, 2048, 2536, 3027
FriOI GRGCYC 4 cut(s) 42, 736, 906, 1388
Fsp4HI GCNGC 8 cut(s) 117, 241, 525, 563, 739, 1046, 1892, 2259
FspBI CTAG 6 cut(s) 59, 996, 1775, 1826, 2543, 2936
GlaI GCGC 1 cut(s) 1730
GluI GCNGC 8 cut(s) 117, 241, 525, 563, 739, 1046, 1892, 2259
GsaI CCCAGC 1 cut(s) 2222
GsuI CTGGAG 2 cut(s) 53, 2874
HaeIII GGCC 7 cut(s) 924, 1772, 1891, 1933, 2097, 2230, 3036
HapII CCGG 6 cut(s) 167, 343, 448, 1018, 1888, 3037
HgaI GACGC 1 cut(s) 104
HhaI GCGC 1 cut(s) 1731
Hin1I GRCGYC 1 cut(s) 96
Hin6I GCGC 1 cut(s) 1729
HinP1I GCGC 1 cut(s) 1729
HincII GTYRAC 3 cut(s) 2180, 2431, 2646
HindII GTYRAC 3 cut(s) 2180, 2431, 2646
HindIII AAGCTT 1 cut(s) 1000
HpaII CCGG 6 cut(s) 167, 343, 448, 1018, 1888, 3037
HphI GGTGA 5 cut(s) 60, 70, 2580, 2911, 2999
Hpy99I CGWCG 1 cut(s) 98
HpyCH4III ACNGT 5 cut(s) 979, 1097, 1288, 1525, 1765
HpyCH4IV ACGT 4 cut(s) 1291, 2046, 2133, 2812
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 161, 2570
HpySE526I ACGT 4 cut(s) 1291, 2046, 2133, 2812
Hsp92I GRCGYC 1 cut(s) 96
HspAI GCGC 1 cut(s) 1729
Kpn2I TCCGGA 1 cut(s) 447
KpnI GGTACC 1 cut(s) 349
Kzo9I GATC 8 cut(s) 108, 286, 1633, 1669, 1905, 2908, 2932, 3064
LmnI GCTCC 3 cut(s) 37, 169, 2353
Lsp1109I GCAGC 5 cut(s) 128, 536, 574, 1032, 2245
LweI GCATC 5 cut(s) 1417, 1893, 2573, 2713, 2980
MaeI CTAG 6 cut(s) 59, 996, 1775, 1826, 2543, 2936
MaeII ACGT 4 cut(s) 1291, 2046, 2133, 2812
MaeIII GTNAC 9 cut(s) 148, 475, 859, 1021, 1271, 1294, 1344, 1869, 2362
MalI GATC 8 cut(s) 110, 288, 1635, 1671, 1907, 2910, 2934, 3066
MboI GATC 8 cut(s) 108, 286, 1633, 1669, 1905, 2908, 2932, 3064
MfeI CAATTG 3 cut(s) 1950, 2298, 3069
MflI RGATCY 4 cut(s) 286, 1669, 2932, 3064
MhlI GDGCHC 4 cut(s) 42, 736, 906, 1388
MlyI GAGTC 1 cut(s) 2002
MmeI TCCRAC 5 cut(s) 998, 1012, 1202, 1893, 3079
Mph1103I ATGCAT 2 cut(s) 1079, 1626
MroI TCCGGA 1 cut(s) 447
MroXI GAANNNNTTC 3 cut(s) 969, 1323, 1743
MslI CAYNNNNRTG 1 cut(s) 1072
MspA1I CMGCKG 4 cut(s) 20, 565, 1894, 2914
MspCI CTTAAG 1 cut(s) 1156
MspI CCGG 6 cut(s) 167, 343, 448, 1018, 1888, 3037
MspR9I CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2853, 3038
MunI CAATTG 3 cut(s) 1950, 2298, 3069
Mva1269I GAATGC 4 cut(s) 543, 597, 1970, 2824
MvaI CCWGG 4 cut(s) 498, 1123, 1300, 2853
MwoI GCNNNNNNNGC 3 cut(s) 37, 161, 2570
NciI CCSGG 3 cut(s) 168, 1019, 3038
NdeI CATATG 1 cut(s) 2224
NdeII GATC 8 cut(s) 108, 286, 1633, 1669, 1905, 2908, 2932, 3064
NheI GCTAGC 1 cut(s) 1825
NlaIV GGNNCC 7 cut(s) 347, 362, 818, 1663, 2381, 3035, 3066
NmuCI GTSAC 5 cut(s) 475, 1271, 1294, 1344, 2362
NsiI ATGCAT 2 cut(s) 1079, 1626
NspI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2022
NspV TTCGAA 1 cut(s) 2701
PaeI GCATGC 1 cut(s) 1077
PaeR7I CTCGAG 1 cut(s) 1040
PagI TCATGA 1 cut(s) 1560
PaqCI CACCTGC 1 cut(s) 79
PasI CCCWGGG 1 cut(s) 497
PceI AGGCCT 1 cut(s) 2097
PciI ACATGT 2 cut(s) 642, 2018
PctI GAATGC 4 cut(s) 543, 597, 1970, 2824
PdmI GAANNNNTTC 3 cut(s) 969, 1323, 1743
PfeI GAWTC 9 cut(s) 171, 965, 1055, 1319, 1494, 1778, 2202, 2675, 2975
PflFI GACNNNGTC 1 cut(s) 1151
PflMI CCANNNNNTGG 1 cut(s) 2225
PfoI TCCNGGA 1 cut(s) 166
PinAI ACCGGT 1 cut(s) 342
PkrI GCNGC 8 cut(s) 118, 242, 526, 564, 740, 1047, 1893, 2260
PleI GAGTC 1 cut(s) 2001
PmaCI CACGTG 2 cut(s) 2134, 2813
PmlI CACGTG 2 cut(s) 2134, 2813
PpsI GAGTC 1 cut(s) 2001
Ppu21I YACGTR 3 cut(s) 1292, 2134, 2813
PscI ACATGT 2 cut(s) 642, 2018
Psp124BI GAGCTC 2 cut(s) 42, 1388
Psp6I CCWGG 4 cut(s) 496, 1121, 1298, 2851
PspCI CACGTG 2 cut(s) 2134, 2813
PspFI CCCAGC 1 cut(s) 2218
PspGI CCWGG 4 cut(s) 496, 1121, 1298, 2851
PspN4I GGNNCC 7 cut(s) 347, 362, 818, 1663, 2381, 3035, 3066
PstI CTGCAG 1 cut(s) 564
PstNI CAGNNNCTG 1 cut(s) 2030
PsuI RGATCY 4 cut(s) 286, 1669, 2932, 3064
PsyI GACNNNGTC 1 cut(s) 1151
RsaI GTAC 9 cut(s) 347, 368, 389, 589, 620, 988, 1290, 2023, 2948
RsaNI GTAC 9 cut(s) 346, 367, 388, 588, 619, 987, 1289, 2022, 2947
RseI CAYNNNNRTG 1 cut(s) 1072
SacI GAGCTC 2 cut(s) 42, 1388
SatI GCNGC 8 cut(s) 117, 241, 525, 563, 739, 1046, 1892, 2259
Sau3AI GATC 8 cut(s) 108, 286, 1633, 1669, 1905, 2908, 2932, 3064
ScaI AGTACT 2 cut(s) 389, 2948
SchI GAGTC 1 cut(s) 2002
ScrFI CCNGG 7 cut(s) 168, 498, 1019, 1123, 1300, 2853, 3038
SduI GDGCHC 4 cut(s) 42, 736, 906, 1388
SfaNI GCATC 5 cut(s) 1417, 1893, 2573, 2713, 2980
SfcI CTRYAG 4 cut(s) 252, 560, 1328, 1761
Sfr274I CTCGAG 1 cut(s) 1040
SfuI TTCGAA 1 cut(s) 2701
SinI GGWCC 7 cut(s) 22, 52, 426, 1421, 2409, 2577, 2665
SlaI CTCGAG 1 cut(s) 1040
SmiMI CAYNNNNRTG 1 cut(s) 1072
SmlI CTYRAG 3 cut(s) 143, 1040, 1156
SmoI CTYRAG 3 cut(s) 143, 1040, 1156
SphI GCATGC 1 cut(s) 1077
SseBI AGGCCT 1 cut(s) 2097
SsiI CCGC 9 cut(s) 20, 240, 522, 565, 739, 1823, 1892, 2912, 3145
SspI AATATT 2 cut(s) 65, 852
SspMI CTAG 6 cut(s) 59, 996, 1775, 1826, 2543, 2936
SstI GAGCTC 2 cut(s) 42, 1388
StuI AGGCCT 1 cut(s) 2097
StyD4I CCNGG 7 cut(s) 166, 496, 1017, 1121, 1298, 2851, 3036
StyI CCWWGG 2 cut(s) 306, 3140
TaaI ACNGT 5 cut(s) 979, 1097, 1288, 1525, 1765
TaiI ACGT 4 cut(s) 1294, 2049, 2136, 2815
TaqI TCGA 6 cut(s) 107, 123, 1041, 1718, 2701, 2907
TaqII GACCGA 1 cut(s) 1290
TatI WGTACW 6 cut(s) 387, 587, 618, 986, 2021, 2946
TauI GCSGC 3 cut(s) 243, 741, 1894
TfiI GAWTC 9 cut(s) 171, 965, 1055, 1319, 1494, 1778, 2202, 2675, 2975
TscAI CASTG 8 cut(s) 1074, 1102, 1368, 1386, 1770, 2035, 2371, 2638
TseFI GTSAC 5 cut(s) 475, 1271, 1294, 1344, 2362
TseI GCWGC 5 cut(s) 116, 524, 562, 1045, 2258
Tsp45I GTSAC 5 cut(s) 475, 1271, 1294, 1344, 2362
TspGWI ACGGA 1 cut(s) 1448
TspRI CASTG 8 cut(s) 1074, 1102, 1368, 1386, 1770, 2035, 2371, 2638
Tth111I GACNNNGTC 1 cut(s) 1151
Van91I CCANNNNNTGG 1 cut(s) 2225
Vha464I CTTAAG 1 cut(s) 1156
VpaK11BI GGWCC 7 cut(s) 22, 52, 426, 1421, 2409, 2577, 2665
XapI RAATTY 7 cut(s) 203, 869, 1374, 1454, 1710, 2079, 2582
XbaI TCTAGA 2 cut(s) 995, 2935
XceI RCATGY 5 cut(s) 646, 1077, 1312, 1583, 2022
XcmI CCANNNNNNNNNTGG 1 cut(s) 2656
XhoI CTCGAG 1 cut(s) 1040
XmiI GTMKAC 2 cut(s) 90, 608
XmnI GAANNNNTTC 3 cut(s) 969, 1323, 1743
XspI CTAG 6 cut(s) 59, 996, 1775, 1826, 2543, 2936
ZrmI AGTACT 2 cut(s) 389, 2948
Zsp2I ATGCAT 2 cut(s) 1079, 1626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.