MD02G1146900.v1.1

Nucleolar protein 6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008672
Physical Location & Seq
Forward (+)
12146555 .. 12154582
8028 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1146900.v1.1.491

Sequence Viewer

Length: 3156 bp
ATGGAGCCCGCTACGCTGACGAACTCAATGGACCTAAAGATTGGGGAGCTCTTGAAAGAGGTCCAGCTCGACTATTCACCCGCATTCACCAAGGCCGTCGACGACGCCGTTTCAGTCATCAAAAGAGCCATCGACAAAATCCCAGAAGGCCTCAATGTTACAGCTGATGAAGCGCGGGGATTCGTCAGGGACATTGGCGCTGACAAGGTCGAATTCACGTTTAAGAAGCCGAAATCTATTGAAATTGGTGGGAGTTATGCCATACAGTGCATTGTGAAACCTGAAGTTAATGTGGACCTCTTCGTTCGGATGCCCAAGGAGTGTTTCCATGAGAAAGACTATTTGAATTACCGGTACCATACGAAAAGGTGCCTGTACCTTTGTGTAATAAAAAAGTTTTTAAAGTCTTCTTCATTAGTTGAATGGTCCACCTTACAGAATGAAGCCCGGAAACCTGTATTGATTGTCTATCCAGGCGTAAAACCTGTCGAAGTCCCTGAGTTTTGTGTAAGGATAATTCCTACAGCACCGTCCCTTTTCAACATTCCAAAGTTGCACCTAGAGCGGAATAATGTTCGTGCTCTAAATCAAGGGGGAGTTCCTCAGGCTACTCCTAAGTACAATAGTAGTATTTTGGAAGACATGCTTATTGAAGATACTGAAGAAATTCTCAAGAAAACTTTTCTCGGGTGGAAAGAATTACAAGAGGCATTAATTTTGCTGAAGGTCTGGGCTCGTCGGAGAACACCAATATATGCTTATGATTGCTTGGGCGGATATCTGATCTCTGTCATATTGTCGTACCTTGCGGATAGGAATCGTATTAAAAAGTCAATGACGACAATGCACATATTCCGTTTTACTCTGAGCTTCCTTGCCAATTCAGAACTTTGGAAACATGGGCTCTACTTTACACCGAAAGACCAAAAAGCTATTTCAAAGGAGAAAAGGTTGCCTCTTAAAGAATCATTTCCAGTTGTCATATGTAGTCCAGCTACAAACTTTAATTTGGCCTTCCGAATGACAAGGGCTGGATTTCTTGAGCTCCAAGATGAGTCTGCTTCGACACTTGCATGCATAGACAAATGTAGAGATGGTGGATTTGAAGAAATTTTTATGACCACGGTGGACTATCCTGTAAAATATGACCACATCATAAGGTTGAACTTAAAAGGGAACAGTGCAGTTTATGCATCAGGATTTTGCTTGGATGATGAATGTTGGAGATTGTATGAGCAGAAGGTGCAGAGTGTTTTAATTCAAGGGTTGGGTGACAGAGTGAAGAATGTACGTGTTACCTGGAGAAATATGTTATCAGACCGCATCATAAAAAATGGATTGTCTACCCTCAATGCAGAGCCATTGCTTATTGGAATTTCCGTGAGCTCCATAGAGAAAGCTTTTAGGCTTGTAGATATTGGTCCAGATGCTGATAACAAAGAGGAGGCCCTCAAATTCCGCAAGTTCTGGGGGGAAAAGTCGGAGCTCAGGAGATTTAAAGACGGTAAAATTGCAGAAAGCACAGTTTGGGAAAGTGAGCAATGGAAAAGACATATCGTCTTAAAAAGGATTTCTGAGTATGTGCTTCTGCGACATCTTTCTGTATCAAAAGAGAATATAATGCACATTGTGGATCAACTTGATTTCTCTTTGCTATATGGTGCTGGAGATCCTATATCATCTTCTGGGAATTTGCTTGGGGCATTTGAAATTCTATCGAAGCGCTTGCGGCTTCTTGAAGACATTCCACTGAAGGTTTCTACTGTGCAGCCCCTAGATTCAGCTTTCAGGTTTTCATCTGTATTCCCACCTGAACCTCATCCGCTTGCTAATGAGAAGGGTGCTTTTGTAAGATTACATAGGTTCACTCCTTCCTGCATTCGTCCGCTGGAAGTTATGATCCAGTTGGAAGGTTCTGGAAACTGGCCAATGGATGATGTAGCAATTGAGAAAACTAAATCTGCCTTCCTTCTCAAGATTGGAGAGAGTCTCCAGAATAATTGGGGGATGACGTGTACAGCTACGGAGGATGATGTGGATGTTTTTGTTTCTGGATATGCATTTCGCCTAAAAATTTGGCATGAGAGAGGCCTGACTTTGTTGAGACGGGAAACTGGAAATGATCAAGTGAAGCAGGTCTCTAATACGGACAGAGAACTTTATTTCCGGAGTCAACATTCCAGCATGATTAATGGATTACAGGGTTGTTACGCAGCATACGGGCCAGTTGTTAGGCTTGCAAAACGGTGGGTAGCTTCACATCTGTTTTCGGCTTGCTTGGGGGAGGAGGCAATTGAACTATTGGTTGCATACGTCTTTTTGAAGCCTTTACCATTCAATGCTCCATGCTCACGAATCACTGGATTTTTGAGGTTCCTGCGATTACTATCAGATTATGACTGGAATTTTTCTGCATTAATTGTCGACATAAACAATGATTTGACTCCAAAAGATATGAAAGAGATAAATGATAATTTCATGTCAAGTAGAAAAACTAATGAAGAAAATGTGCAAAGCGTAAACCCAGCAATGTTCTTGGCTACAACTTACGACAAGGCATCCGATGCCTGGACCAGATTCTCACCAAACTCGATGTTAAAAAGGTTGATGGCTTATGCTGGAAGCAGTGCAAAGTTGTTGACCAAACTGGTATCAGAGGATCATGATGATAAGCATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAATTACGACGCAGTTATTCTTCTTCACAGAGAAAAATTACCTTACCCCCAACATCTTCTCTTTCCATCCGAATTAAATCAAGGTGCAGGGGTACATGTGGCATGTGGTAATGCGAGCAAGGTTTTCCACCCATTTCTGTTGCCTGGAGACATCAAAGGAAATTCGGAGGCACTAAGAAATAAGCTATTGGTAAACTTTGATCCACTGAGGTGCTTTGTCGGGGATCTAGAGAAAGAGTACCCCAACACCTTCAAGCTATGGTATGATTGTCTGGGTGGTGATGCTGTTGGCATAACGTGGGGGAGATATGGTTCAAAGAAACGAGGGCGGGAAGAAGAAGCAGAGGAGGTAAAAGATCCAACTGGTTTATTAAAAAACATTGGTGAAGTAGGAACAGGATTTGTGAGAGGGGTTTATCTTCTCAAGGCCCCAAGGCTCACGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

1052

Amino Acids

119.54

Weight (kDa)

8.55

Isoelectric Point (pI)

43.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nrap PF03813 98 - 227 3e-35 Nrap protein domain 1
Nrap_D2 PF17403 234 - 374 2.7e-28 Nrap protein PAP/OAS-like domain
Nrap_D3 PF17404 380 - 534 9.7e-38 Nrap protein domain 3
Nrap_D4 PF17405 558 - 734 3e-46 Nrap protein nucleotidyltransferase domain 4
Nrap_D5 PF17406 737 - 899 1.5e-46 Nrap protein PAP/OAS1-like domain 5
Nrap_D6 PF17407 902 - 1039 7e-12 Nrap protein domain 6
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2125
Acc65I GGTACC 1 cut(s) 354
AccB1I GGYRCC 2 cut(s) 354, 369
AccBSI CCGCTC 1 cut(s) 565
AccI GTMKAC 3 cut(s) 99, 1343, 2424
AccII CGCG 1 cut(s) 175
AccIII TCCGGA 1 cut(s) 2166
AclWI GGATC 7 cut(s) 1641, 1664, 1894, 2667, 2903, 2940, 3059
AcoI YGGCCR 1 cut(s) 1925
AcuI CTGAAG 4 cut(s) 303, 681, 743, 1772
AcyI GRCGYC 1 cut(s) 105
AdeI CACNNNGTG 1 cut(s) 1630
AfaI GTAC 8 cut(s) 356, 377, 620, 803, 1290, 2017, 2802, 2948
AfeI AGCGCT 1 cut(s) 1724
AfiI CCNNNNNNNGG 1 cut(s) 2568
AflIII ACRYGT 3 cut(s) 1291, 2012, 2803
AgeI ACCGGT 1 cut(s) 351
AhdI GACNNNNNGTC 1 cut(s) 1556
AjiI CACGTC 1 cut(s) 2013
AjnI CCWGG 4 cut(s) 472, 1298, 2567, 2851
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AleI CACNNNNGTG 1 cut(s) 2917
AloI GAACNNNNNNTCC 4 cut(s) 2148, 2180, 3004, 3036
Alw21I GWGCWC 5 cut(s) 51, 583, 1047, 1388, 1488
Alw26I GTCTC 4 cut(s) 1994, 2098, 2143, 2850
AlwI GGATC 7 cut(s) 1641, 1664, 1894, 2667, 2903, 2940, 3059
AlwNI CAGNNNCTG 1 cut(s) 1430
Ama87I CYCGRG 1 cut(s) 686
Aor13HI TCCGGA 1 cut(s) 2166
Aor51HI AGCGCT 1 cut(s) 1724
AoxI GGCC 8 cut(s) 93, 148, 1011, 1446, 1925, 2089, 2222, 3135
ApeKI GCWGC 2 cut(s) 1768, 2213
AseI ATTAAT 3 cut(s) 713, 2190, 2417
AsiGI ACCGGT 1 cut(s) 351
Asp700I GAANNNNTTC 3 cut(s) 666, 969, 1743
Asp718I GGTACC 1 cut(s) 354
AspLEI GCGC 3 cut(s) 175, 200, 1725
AspS9I GGNCC 9 cut(s) 31, 61, 295, 426, 1421, 1447, 2222, 2571, 3136
AsuC2I CCSGG 1 cut(s) 448
AsuHPI GGTGA 6 cut(s) 69, 79, 1283, 2574, 2999, 3104
AsuII TTCGAA 1 cut(s) 2695
AvaI CYCGRG 1 cut(s) 686
AvaII GGWCC 6 cut(s) 31, 61, 295, 426, 1421, 2571
AxyI CCTNAGG 1 cut(s) 603
BalI TGGCCA 1 cut(s) 1927
BanI GGYRCC 2 cut(s) 354, 369
BanII GRGCYC 7 cut(s) 9, 51, 736, 906, 1047, 1388, 1488
BauI CACGAG 1 cut(s) 3148
BbsI GAAGAC 3 cut(s) 399, 645, 1746
Bbv12I GWGCWC 5 cut(s) 51, 583, 1047, 1388, 1488
BbvI GCAGC 2 cut(s) 1780, 2225
BccI CCATC 5 cut(s) 137, 1088, 2602, 2673, 2782
BceAI ACGGC 2 cut(s) 80, 92
BcgI CGANNNNNNTGC 2 cut(s) 1708, 1742
BciT130I CCWGG 4 cut(s) 474, 1300, 2569, 2853
BclI TGATCA 1 cut(s) 2122
BcnI CCSGG 1 cut(s) 448
BcoDI GTCTC 4 cut(s) 1994, 2098, 2143, 2850
BfaI CTAG 3 cut(s) 560, 1775, 2936
BfmI CTRYAG 1 cut(s) 522
BfoI RGCGCY 2 cut(s) 201, 1726
BfuAI ACCTGC 1 cut(s) 2125
BisI GCNGC 3 cut(s) 1730, 1769, 2214
BlsI GCNGC 3 cut(s) 1731, 1770, 2215
Bme1390I CCNGG 5 cut(s) 448, 474, 1300, 2569, 2853
Bme18I GGWCC 6 cut(s) 31, 61, 295, 426, 1421, 2571
BmeRI GACNNNNNGTC 1 cut(s) 1556
BmeT110I CYCGRG 1 cut(s) 686
BmgBI CACGTC 1 cut(s) 2013
BmgT120I GGNCC 9 cut(s) 31, 61, 295, 426, 1421, 1447, 2222, 2571, 3136
BmiI GGNNCC 5 cut(s) 6, 356, 371, 2375, 3138
BmrFI CCNGG 5 cut(s) 448, 474, 1300, 2569, 2853
BmsI GCATC 7 cut(s) 300, 1202, 1332, 1417, 2554, 2567, 2980
BpiI GAAGAC 3 cut(s) 399, 645, 1746
BplI GAGNNNNNCTC 2 cut(s) 1974, 2006
BpmI CTGGAG 4 cut(s) 1321, 1686, 1976, 2874
Bpu10I CCTNAGC 1 cut(s) 1487
Bpu14I TTCGAA 1 cut(s) 2695
BpuEI CTTGAG 4 cut(s) 656, 1061, 1958, 3116
BpuMI CCSGG 1 cut(s) 448
BsaAI YACGTR 1 cut(s) 1292
BsaBI GATNNNNATC 2 cut(s) 816, 2386
BsaHI GRCGYC 1 cut(s) 105
BsaI GGTCTC 1 cut(s) 2143
BsaJI CCNNGG 4 cut(s) 90, 315, 1122, 3140
BsaWI WCCGGW 2 cut(s) 351, 2166
BsaXI ACNNNNNCTCC 4 cut(s) 935, 965, 3004, 3034
Bsc4I CCNNNNNNNGG 1 cut(s) 2568
Bse118I RCCGGY 1 cut(s) 351
Bse1I ACTGG 9 cut(s) 974, 1903, 1928, 2119, 2225, 2365, 2405, 2652, 3076
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 3 cut(s) 1361, 1547, 2535
Bse8I GATNNNNATC 2 cut(s) 816, 2386
BseAI TCCGGA 1 cut(s) 2166
BseBI CCWGG 4 cut(s) 474, 1300, 2569, 2853
BseDI CCNNGG 4 cut(s) 90, 315, 1122, 3140
BseGI GGATG 9 cut(s) 315, 1216, 1819, 1939, 2013, 2035, 2044, 2558, 2774
BseJI GATNNNNATC 2 cut(s) 816, 2386
BseLI CCNNNNNNNGG 1 cut(s) 2568
BseMI GCAATG 3 cut(s) 1361, 1547, 2535
BseMII CTCAG 6 cut(s) 489, 617, 857, 1501, 1566, 2906
BseNI ACTGG 9 cut(s) 974, 1903, 1928, 2119, 2225, 2365, 2405, 2652, 3076
BseRI GAGGAG 3 cut(s) 1457, 2300, 3068
BseXI GCAGC 2 cut(s) 1780, 2225
BseYI CCCAGC 1 cut(s) 2524
BsgI GTGCAG 4 cut(s) 1203, 1265, 1787, 2814
Bsh1236I CGCG 1 cut(s) 175
BshFI GGCC 8 cut(s) 95, 150, 1013, 1448, 1927, 2091, 2224, 3137
BshNI GGYRCC 2 cut(s) 354, 369
BshTI ACCGGT 1 cut(s) 351
BsiHKAI GWGCWC 5 cut(s) 51, 583, 1047, 1388, 1488
BsiHKCI CYCGRG 1 cut(s) 686
BsiSI CCGG 3 cut(s) 352, 448, 2167
BslFI GGGAC 3 cut(s) 203, 479, 517
BslI CCNNNNNNNGG 1 cut(s) 2568
BsmAI GTCTC 4 cut(s) 1994, 2098, 2143, 2850
BsmBI CGTCTC 1 cut(s) 2098
BsmFI GGGAC 3 cut(s) 203, 479, 517
BsmI GAATGC 2 cut(s) 83, 1878
BsnI GGCC 8 cut(s) 95, 150, 1013, 1448, 1927, 2091, 2224, 3137
Bso31I GGTCTC 1 cut(s) 2143
BsoBI CYCGRG 1 cut(s) 686
Bsp119I TTCGAA 1 cut(s) 2695
Bsp1286I GDGCHC 8 cut(s) 9, 51, 583, 736, 906, 1047, 1388, 1488
Bsp13I TCCGGA 1 cut(s) 2166
Bsp1407I TGTACA 1 cut(s) 2015
Bsp143I GATC 9 cut(s) 783, 1633, 1669, 1899, 2122, 2659, 2908, 2932, 3064
BspANI GGCC 8 cut(s) 95, 150, 1013, 1448, 1927, 2091, 2224, 3137
BspCNI CTCAG 6 cut(s) 490, 616, 858, 1500, 1567, 2907
BspEI TCCGGA 1 cut(s) 2166
BspFNI CGCG 1 cut(s) 175
BspHI TCATGA 1 cut(s) 2662
BspLI GGNNCC 5 cut(s) 6, 356, 371, 2375, 3138
BspMI ACCTGC 1 cut(s) 2125
BspPI GGATC 7 cut(s) 1641, 1664, 1894, 2667, 2903, 2940, 3059
BspT104I TTCGAA 1 cut(s) 2695
BspT107I GGYRCC 2 cut(s) 354, 369
BspTNI GGTCTC 1 cut(s) 2143
BsrBI CCGCTC 1 cut(s) 565
BsrDI GCAATG 3 cut(s) 1361, 1547, 2535
BsrFI RCCGGY 1 cut(s) 351
BsrGI TGTACA 1 cut(s) 2015
BsrI ACTGG 9 cut(s) 974, 1903, 1928, 2119, 2225, 2365, 2405, 2652, 3076
BssAI RCCGGY 1 cut(s) 351
BssECI CCNNGG 4 cut(s) 90, 315, 1122, 3140
BssMI GATC 9 cut(s) 783, 1633, 1669, 1899, 2122, 2659, 2908, 2932, 3064
BssNI GRCGYC 1 cut(s) 105
BssSI CACGAG 1 cut(s) 3148
BssT1I CCWWGG 3 cut(s) 90, 315, 3140
Bst2BI CACGAG 1 cut(s) 3148
Bst2UI CCWGG 4 cut(s) 474, 1300, 2569, 2853
Bst4CI ACNGT 8 cut(s) 267, 531, 1126, 1181, 1505, 1525, 1765, 2247
Bst6I CTCTTC 1 cut(s) 305
BstACI GRCGYC 1 cut(s) 105
BstAPI GCANNNNNTGC 3 cut(s) 1190, 1243, 2564
BstAUI TGTACA 1 cut(s) 2015
BstBAI YACGTR 1 cut(s) 1292
BstBI TTCGAA 1 cut(s) 2695
BstC8I GCNNGC 7 cut(s) 9, 1075, 1727, 1827, 2238, 2275, 2824
BstDEI CTNAG 8 cut(s) 498, 603, 615, 866, 1487, 1575, 2882, 2915
BstDSI CCRYGG 1 cut(s) 1122
BstF5I GGATG 9 cut(s) 315, 1216, 1819, 1939, 2013, 2035, 2044, 2558, 2774
BstFNI CGCG 1 cut(s) 175
BstH2I RGCGCY 2 cut(s) 201, 1726
BstHHI GCGC 3 cut(s) 175, 200, 1725
BstKTI GATC 9 cut(s) 786, 1636, 1672, 1902, 2125, 2662, 2911, 2935, 3067
BstMAI GTCTC 4 cut(s) 1994, 2098, 2143, 2850
BstMBI GATC 9 cut(s) 783, 1633, 1669, 1899, 2122, 2659, 2908, 2932, 3064
BstMWI GCNNNNNNNGC 7 cut(s) 13, 170, 562, 1190, 1243, 1729, 2564
BstNI CCWGG 4 cut(s) 474, 1300, 2569, 2853
BstNSI RCATGY 4 cut(s) 646, 1077, 2807, 2814
BstSCI CCNGG 5 cut(s) 446, 472, 1298, 2567, 2851
BstSFI CTRYAG 1 cut(s) 522
BstUI CGCG 1 cut(s) 175
BstV1I GCAGC 2 cut(s) 1780, 2225
BstV2I GAAGAC 3 cut(s) 399, 645, 1746
BstX2I RGATCY 3 cut(s) 1669, 2932, 3064
BstYI RGATCY 3 cut(s) 1669, 2932, 3064
Bsu36I CCTNAGG 1 cut(s) 603
BsuRI GGCC 8 cut(s) 95, 150, 1013, 1448, 1927, 2091, 2224, 3137
BtgI CCRYGG 1 cut(s) 1122
BtrI CACGTC 1 cut(s) 2013
BtsCI GGATG 9 cut(s) 315, 1216, 1819, 1939, 2013, 2035, 2044, 2558, 2774
BtsI GCAGTG 1 cut(s) 2632
BtsIMutI CAGTG 6 cut(s) 272, 1186, 1748, 2358, 2632, 2912
BveI ACCTGC 1 cut(s) 2125
Cac8I GCNNGC 7 cut(s) 9, 1075, 1727, 1827, 2238, 2275, 2824
CaiI CAGNNNCTG 1 cut(s) 1430
CciI TCATGA 1 cut(s) 2662
CfoI GCGC 3 cut(s) 175, 200, 1725
Cfr10I RCCGGY 1 cut(s) 351
Cfr13I GGNCC 9 cut(s) 31, 61, 295, 426, 1421, 1447, 2222, 2571, 3136
CseI GACGC 2 cut(s) 113, 2726
Csp6I GTAC 8 cut(s) 355, 376, 619, 802, 1289, 2016, 2801, 2947
CspAI ACCGGT 1 cut(s) 351
CviQI GTAC 8 cut(s) 355, 376, 619, 802, 1289, 2016, 2801, 2947
DdeI CTNAG 8 cut(s) 498, 603, 615, 866, 1487, 1575, 2882, 2915
DpnI GATC 9 cut(s) 785, 1635, 1671, 1901, 2124, 2661, 2910, 2934, 3066
DpnII GATC 9 cut(s) 783, 1633, 1669, 1899, 2122, 2659, 2908, 2932, 3064
DraI TTTAAA 2 cut(s) 402, 1498
DraIII CACNNNGTG 1 cut(s) 1630
DriI GACNNNNNGTC 1 cut(s) 1556
EaeI YGGCCR 1 cut(s) 1925
Eam1104I CTCTTC 1 cut(s) 305
Eam1105I GACNNNNNGTC 1 cut(s) 1556
EarI CTCTTC 1 cut(s) 305
EciI GGCGGA 1 cut(s) 789
Ecl136II GAGCTC 4 cut(s) 49, 1045, 1386, 1486
Eco130I CCWWGG 3 cut(s) 90, 315, 3140
Eco147I AGGCCT 2 cut(s) 150, 2091
Eco24I GRGCYC 7 cut(s) 9, 51, 736, 906, 1047, 1388, 1488
Eco31I GGTCTC 1 cut(s) 2143
Eco32I GATATC 1 cut(s) 779
Eco47I GGWCC 6 cut(s) 31, 61, 295, 426, 1421, 2571
Eco47III AGCGCT 1 cut(s) 1724
Eco53kI GAGCTC 4 cut(s) 49, 1045, 1386, 1486
Eco57I CTGAAG 4 cut(s) 303, 681, 743, 1772
Eco81I CCTNAGG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 686
EcoICRI GAGCTC 4 cut(s) 49, 1045, 1386, 1486
EcoO109I RGGNCCY 2 cut(s) 1447, 3136
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 4 cut(s) 472, 1298, 2567, 2851
EcoRV GATATC 1 cut(s) 779
EcoT14I CCWWGG 3 cut(s) 90, 315, 3140
EcoT22I ATGCAT 3 cut(s) 1079, 1195, 2062
EcoT38I GRGCYC 7 cut(s) 9, 51, 736, 906, 1047, 1388, 1488
ErhI CCWWGG 3 cut(s) 90, 315, 3140
Esp3I CGTCTC 1 cut(s) 2098
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 3 cut(s) 203, 479, 517
FauI CCCGC 4 cut(s) 16, 88, 168, 3030
FauNDI CATATG 1 cut(s) 983
FbaI TGATCA 1 cut(s) 2122
FblI GTMKAC 3 cut(s) 99, 1343, 2424
Fnu4HI GCNGC 3 cut(s) 1730, 1769, 2214
FokI GGATG 9 cut(s) 322, 1223, 1806, 1946, 2020, 2042, 2051, 2545, 2761
FriOI GRGCYC 7 cut(s) 9, 51, 736, 906, 1047, 1388, 1488
Fsp4HI GCNGC 3 cut(s) 1730, 1769, 2214
FspBI CTAG 3 cut(s) 560, 1775, 2936
GlaI GCGC 3 cut(s) 174, 199, 1724
GluI GCNGC 3 cut(s) 1730, 1769, 2214
GsaI CCCAGC 1 cut(s) 2528
GsuI CTGGAG 4 cut(s) 1321, 1686, 1976, 2874
HaeII RGCGCY 2 cut(s) 201, 1726
HaeIII GGCC 8 cut(s) 95, 150, 1013, 1448, 1927, 2091, 2224, 3137
HapII CCGG 3 cut(s) 352, 448, 2167
HgaI GACGC 2 cut(s) 113, 2726
HhaI GCGC 3 cut(s) 175, 200, 1725
Hin1I GRCGYC 1 cut(s) 105
Hin6I GCGC 3 cut(s) 173, 198, 1723
HinP1I GCGC 3 cut(s) 173, 198, 1723
HincII GTYRAC 4 cut(s) 100, 2174, 2425, 2640
HindII GTYRAC 4 cut(s) 100, 2174, 2425, 2640
HindIII AAGCTT 1 cut(s) 1398
HpaII CCGG 3 cut(s) 352, 448, 2167
HphI GGTGA 6 cut(s) 69, 79, 1283, 2574, 2999, 3104
Hpy99I CGWCG 5 cut(s) 101, 104, 107, 741, 2720
HpyCH4III ACNGT 8 cut(s) 267, 531, 1126, 1181, 1505, 1525, 1765, 2247
HpyCH4IV ACGT 5 cut(s) 218, 1291, 2012, 2313, 3005
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 170, 562, 1190, 1243, 1729, 2564
HpyF3I CTNAG 8 cut(s) 498, 603, 615, 866, 1487, 1575, 2882, 2915
HpySE526I ACGT 5 cut(s) 218, 1291, 2012, 2313, 3005
Hsp92I GRCGYC 1 cut(s) 105
HspAI GCGC 3 cut(s) 173, 198, 1723
Kpn2I TCCGGA 1 cut(s) 2166
KpnI GGTACC 1 cut(s) 358
Ksp22I TGATCA 1 cut(s) 2122
Kzo9I GATC 9 cut(s) 783, 1633, 1669, 1899, 2122, 2659, 2908, 2932, 3064
LmnI GCTCC 6 cut(s) 4, 46, 1050, 1391, 1483, 2347
Lsp1109I GCAGC 2 cut(s) 1780, 2225
LweI GCATC 7 cut(s) 300, 1202, 1332, 1417, 2554, 2567, 2980
MaeI CTAG 3 cut(s) 560, 1775, 2936
MaeII ACGT 5 cut(s) 218, 1291, 2012, 2313, 3005
MaeIII GTNAC 4 cut(s) 157, 1271, 1294, 2207
MalI GATC 9 cut(s) 785, 1635, 1671, 1901, 2124, 2661, 2910, 2934, 3066
MbiI CCGCTC 1 cut(s) 565
MboI GATC 9 cut(s) 783, 1633, 1669, 1899, 2122, 2659, 2908, 2932, 3064
MfeI CAATTG 2 cut(s) 1944, 2292
MflI RGATCY 3 cut(s) 1669, 2932, 3064
MhlI GDGCHC 8 cut(s) 9, 51, 583, 736, 906, 1047, 1388, 1488
MlsI TGGCCA 1 cut(s) 1927
MluNI TGGCCA 1 cut(s) 1927
MlyI GAGTC 4 cut(s) 1064, 1996, 2179, 2437
MmeI TCCRAC 5 cut(s) 719, 1202, 1461, 1887, 3092
Mox20I TGGCCA 1 cut(s) 1927
Mph1103I ATGCAT 3 cut(s) 1079, 1195, 2062
MroI TCCGGA 1 cut(s) 2166
MroXI GAANNNNTTC 3 cut(s) 666, 969, 1743
MscI TGGCCA 1 cut(s) 1927
MslI CAYNNNNRTG 2 cut(s) 1072, 2917
Msp20I TGGCCA 1 cut(s) 1927
MspA1I CMGCKG 2 cut(s) 164, 1888
MspI CCGG 3 cut(s) 352, 448, 2167
MspR9I CCNGG 5 cut(s) 448, 474, 1300, 2569, 2853
MunI CAATTG 2 cut(s) 1944, 2292
Mva1269I GAATGC 2 cut(s) 83, 1878
MvaI CCWGG 4 cut(s) 474, 1300, 2569, 2853
MvnI CGCG 1 cut(s) 175
MwoI GCNNNNNNNGC 7 cut(s) 13, 170, 562, 1190, 1243, 1729, 2564
NciI CCSGG 1 cut(s) 448
NdeI CATATG 1 cut(s) 983
NdeII GATC 9 cut(s) 783, 1633, 1669, 1899, 2122, 2659, 2908, 2932, 3064
NlaIV GGNNCC 5 cut(s) 6, 356, 371, 2375, 3138
NmuCI GTSAC 1 cut(s) 1271
NsiI ATGCAT 3 cut(s) 1079, 1195, 2062
NspI RCATGY 4 cut(s) 646, 1077, 2807, 2814
NspV TTCGAA 1 cut(s) 2695
OliI CACNNNNGTG 1 cut(s) 2917
PaeI GCATGC 1 cut(s) 1077
PagI TCATGA 1 cut(s) 2662
PceI AGGCCT 2 cut(s) 150, 2091
PciI ACATGT 1 cut(s) 2803
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 2 cut(s) 83, 1878
PdmI GAANNNNTTC 3 cut(s) 666, 969, 1743
PfeI GAWTC 6 cut(s) 180, 817, 965, 1778, 2355, 2577
PflFI GACNNNGTC 1 cut(s) 206
PinAI ACCGGT 1 cut(s) 351
PkrI GCNGC 3 cut(s) 1731, 1770, 2215
PleI GAGTC 4 cut(s) 1063, 1995, 2178, 2437
PpsI GAGTC 4 cut(s) 1063, 1995, 2178, 2437
Ppu21I YACGTR 1 cut(s) 1292
PscI ACATGT 1 cut(s) 2803
PshBI ATTAAT 3 cut(s) 713, 2190, 2417
Psp124BI GAGCTC 4 cut(s) 51, 1047, 1388, 1488
Psp6I CCWGG 4 cut(s) 472, 1298, 2567, 2851
PspFI CCCAGC 1 cut(s) 2524
PspGI CCWGG 4 cut(s) 472, 1298, 2567, 2851
PspN4I GGNNCC 5 cut(s) 6, 356, 371, 2375, 3138
PspPI GGNCC 9 cut(s) 31, 61, 295, 426, 1421, 1447, 2222, 2571, 3136
PstNI CAGNNNCTG 1 cut(s) 1430
PsuI RGATCY 3 cut(s) 1669, 2932, 3064
PsyI GACNNNGTC 1 cut(s) 206
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 8 cut(s) 356, 377, 620, 803, 1290, 2017, 2802, 2948
RsaNI GTAC 8 cut(s) 355, 376, 619, 802, 1289, 2016, 2801, 2947
RseI CAYNNNNRTG 2 cut(s) 1072, 2917
SacI GAGCTC 4 cut(s) 51, 1047, 1388, 1488
SalI GTCGAC 2 cut(s) 98, 2423
SatI GCNGC 3 cut(s) 1730, 1769, 2214
Sau3AI GATC 9 cut(s) 783, 1633, 1669, 1899, 2122, 2659, 2908, 2932, 3064
Sau96I GGNCC 9 cut(s) 31, 61, 295, 426, 1421, 1447, 2222, 2571, 3136
SchI GAGTC 4 cut(s) 1064, 1996, 2179, 2437
ScrFI CCNGG 5 cut(s) 448, 474, 1300, 2569, 2853
SduI GDGCHC 8 cut(s) 9, 51, 583, 736, 906, 1047, 1388, 1488
SfaNI GCATC 7 cut(s) 300, 1202, 1332, 1417, 2554, 2567, 2980
SfcI CTRYAG 1 cut(s) 522
SfuI TTCGAA 1 cut(s) 2695
SgrDI CGTCGACG 1 cut(s) 98
SinI GGWCC 6 cut(s) 31, 61, 295, 426, 1421, 2571
SmiMI CAYNNNNRTG 2 cut(s) 1072, 2917
SmlI CTYRAG 4 cut(s) 671, 1040, 1973, 3131
SmoI CTYRAG 4 cut(s) 671, 1040, 1973, 3131
SphI GCATGC 1 cut(s) 1077
SseBI AGGCCT 2 cut(s) 150, 2091
SspMI CTAG 3 cut(s) 560, 1775, 2936
SstI GAGCTC 4 cut(s) 51, 1047, 1388, 1488
StuI AGGCCT 2 cut(s) 150, 2091
StyD4I CCNGG 5 cut(s) 446, 472, 1298, 2567, 2851
StyI CCWWGG 3 cut(s) 90, 315, 3140
TaaI ACNGT 8 cut(s) 267, 531, 1126, 1181, 1505, 1525, 1765, 2247
TaiI ACGT 5 cut(s) 221, 1294, 2015, 2316, 3008
TatI WGTACW 2 cut(s) 618, 2015
TauI GCSGC 1 cut(s) 1732
TfiI GAWTC 6 cut(s) 180, 817, 965, 1778, 2355, 2577
TscAI CASTG 6 cut(s) 272, 1186, 1755, 2365, 2632, 2919
TseFI GTSAC 1 cut(s) 1271
TseI GCWGC 2 cut(s) 1768, 2213
Tsp45I GTSAC 1 cut(s) 1271
TspDTI ATGAA 8 cut(s) 183, 402, 456, 1230, 1785, 2467, 2471, 2514
TspGWI ACGGA 4 cut(s) 845, 1369, 2039, 2162
TspRI CASTG 6 cut(s) 272, 1186, 1755, 2365, 2632, 2919
Tth111I GACNNNGTC 1 cut(s) 206
VpaK11BI GGWCC 6 cut(s) 31, 61, 295, 426, 1421, 2571
VspI ATTAAT 3 cut(s) 713, 2190, 2417
XbaI TCTAGA 1 cut(s) 2935
XceI RCATGY 4 cut(s) 646, 1077, 2807, 2814
XmiI GTMKAC 3 cut(s) 99, 1343, 2424
XmnI GAANNNNTTC 3 cut(s) 666, 969, 1743
XspI CTAG 3 cut(s) 560, 1775, 2936
Zsp2I ATGCAT 3 cut(s) 1079, 1195, 2062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.