Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008672

Physical Location & Seq

Genomic Coordinates Forward (+)

22349723 .. 22353810

Gene Structure 4088 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1261100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGCTGATAATAAAGAGGAGGCTCTTTAATTCTGCAAGTTCTGGGGGGAAAAGTCGGAGCTCAGGAGATTTAAAGATGGTAAAATTGCAGAAAGCACAGCAATGGAGAAGACATATTATCTTAAAAGGGATTTCCGAGTATGTGCTTCAGCGACATCTTTCTGTATCAAAAGAGAATATAATGCACATCGTGGATCAACTTGATTTCTCATTGCTATATGGTGCTGGAGATCCTATATCATCTTCTGGGAATTTGCTTGGGGTATTTGAAATTCTATTGAAGCGCTTGCGGCTTCTTGAAGACATTCCACTGAAGGTTTCTACTGTGCAGCCCCTAGATTCAGCTTTCAGGTTTTCATCTGTCTTCCCACCTGAACCTCATCTGCTTGCTAATGAGAAGGGTGCTTTTGTAAGCCTACATAGGTTCACTCCTTCCTGCATTCATCCGCTGGAATTGGAAGGTTCTGGAAACTGGTCAATGGATGATGTAGCAATTGAGAAAACTAAATCTGCCTTCCTTCTCAAGATTGGAGAGAGTCTCCAGAATAATTGGGGGATGACATGTACCGCTACAGAGGATGATGTGGATGTTTTTGTTTCTGGATATGCATTTCGCCTGAAAATTTGGCATGAGAGAGGCCCGACTTTGTTGAGACAGGAAACGGGAAATGATCAAGTGAAGCACGTCTATAATACAGACAGAGAACTTTATTTCCGGAGTCAACATTCCAGCATGATTAATGGATTACAGGGTTTTTATGCAGCATATGGGCCAGTTGTTAGGCTTGCTAAGCAGTGCGTAGCTTCACATCTGTTTTCGGCTTGCTTGAGGGAGGAGGCAATTGAACTATTGGTTGCATACGTCTTTTTGAAGCCTTTACCATTCAGTGCTCCGTGCTCACGAATCACTGGATTTTTGAGGTTCCTGCGATTACTATCAGATTATGACTGGAATTTTTCTGCACTAGTTGTTGACATAAGCAATGATTTGACTCCCAAAGATATGAAAGAGATAAATGAGAATTTTATGTCAAGTAGAAAAACTAATGAAGAAAATGTGCAAAGTGTAAAGCCAGCAATGTTCTTGGCTACAACTTACGACAGGGCATCCGAGGCCTGGACCAGATTCTCAGTAAACTCAATGGAGTTAAAAAGGTTGATGGCTTATGCTGGAAGCAGTGCAAACTTGTTGACCAAACTGATTTCGGAGGATCATAATGATAATTATAGATGGGAGTGCCTTTTTCGAACTCCTTTGAACAGTTACGATGCGGTTATTCTTCTCCACAGAGAAAAATTACCTTACCCCCAACATCTTCTCTTCCCATCTGAATTAAATCAAGGGGTACATGTGGCACGTGGGAATGCGAGCATGGTTTTCCACCCATTTCTGTTGCCTGGAGACATGAAAGGAAACTCGGAGGCACTACGAAATAAGCTATTGAAAGAGTACCCCAACACCTTCAAGCTGTGGAATGATTCTCTGGGTGGTGATGCTGTTGGCATAACGTGGGCGAGATATGGTTCAAAGAAACGAGGGCGGGAAGAAGCAGAGGAAGTAAAAGATCCAACTGATTTATTAAAAGACATCGGTAAAGTGGGAACAGGGTTTGTGAGGGGGGTTTATCTTCTCAAAGCCCCAAGGCTCACTAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Nrap Nrap_D2 Nrap_D3 Nrap_D4 Nrap_D5 Nrap_D6

Protein Analysis

552

Amino Acids

62.4

Weight (kDa)

8.86

Isoelectric Point (pI)

45.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nrap_D4	PF17405	78 - 250	3.8e-38	Nrap protein nucleotidyltransferase domain 4
Nrap_D5	PF17406	253 - 406	3.2e-44	Nrap protein PAP/OAS1-like domain 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008672)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63810 AT1G63810
fragaria_vesca	FvH4_1g13250 FvH4_1g13250
malus_domestica	MD02G1146900.v1.1 MD15G1261100.v1.1
prunus_persica	Prupe.7G155300_v2.0.a1 Prupe.7G155300_v2.0.a1 Prupe.7G155300_v2.0.a1
pyrus_communis	pycom02g11620 pycom15g22840 pycom15g22850
rosa_chinensis	RchiOBHm_Chr2g0101431 RchiOBHm_Chr2g0101471
rosa_laevigata	RLG00000017080
rosa_multiflora	Rmu_sc0006153.1_g000056
rosa_roxburghii	Rroxscaffold_2G00141310
rosa_rugosa	Rorug02G0098300
rosa_samantha	Rh2AG147000 Rh2BG152300 Rh2CG152800 Rh2DG152400
rosa_wichuraiana	Rw2G011460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	1 cut(s)	714
AciI	CCGC	5 cut(s)	289, 446, 567, 1271, 1540
AclWI	GGATC	4 cut(s)	201, 224, 1218, 1559
AcsI	RAATTY	5 cut(s)	250, 270, 621, 952, 1021
AcuI	CTGAAG	2 cut(s)	131, 332
AcvI	CACGTG	1 cut(s)	1358
AdeI	CACNNNGTG	1 cut(s)	190
AfaI	GTAC	3 cut(s)	565, 1347, 1451
AfeI	AGCGCT	1 cut(s)	284
AfiI	CCNNNNNNNGG	1 cut(s)	1116
AflIII	ACRYGT	2 cut(s)	560, 1348
AgsI	TTSAA	9 cut(s)	269, 280, 299, 845, 871, 1258, 1444, 1465, 1527
AhlI	ACTAGT	2 cut(s)	964, 1649
AjiI	CACGTC	1 cut(s)	685
AjnI	CCWGG	2 cut(s)	1115, 1396
AloI	GAACNNNNNNTCC	2 cut(s)	696, 728
AluBI	AGCT	5 cut(s)	60, 344, 803, 1438, 1468
AluI	AGCT	5 cut(s)	60, 344, 803, 1438, 1468
Alw21I	GWGCWC	3 cut(s)	62, 892, 899
Alw26I	GTCTC	3 cut(s)	542, 646, 1395
AlwI	GGATC	4 cut(s)	201, 224, 1218, 1559
Aor13HI	TCCGGA	1 cut(s)	714
Aor51HI	AGCGCT	1 cut(s)	284
AoxI	GGCC	3 cut(s)	637, 770, 1113
ApeKI	GCWGC	2 cut(s)	328, 761
ApoI	RAATTY	5 cut(s)	250, 270, 621, 952, 1021
AseI	ATTAAT	1 cut(s)	738
Asp700I	GAANNNNTTC	1 cut(s)	303
AspLEI	GCGC	1 cut(s)	285
AspS9I	GGNCC	3 cut(s)	638, 770, 1119
AsuHPI	GGTGA	1 cut(s)	1502
AsuII	TTCGAA	1 cut(s)	1246
AvaII	GGWCC	1 cut(s)	1119
BanII	GRGCYC	1 cut(s)	62
BbrPI	CACGTG	1 cut(s)	1358
BbsI	GAAGAC	3 cut(s)	115, 306, 355
Bbv12I	GWGCWC	3 cut(s)	62, 892, 899
BbvI	GCAGC	2 cut(s)	340, 773
BccI	CCATC	4 cut(s)	70, 1153, 1224, 1333
BciT130I	CCWGG	2 cut(s)	1117, 1398
BclI	TGATCA	1 cut(s)	670
BcoDI	GTCTC	3 cut(s)	542, 646, 1395
BcuI	ACTAGT	2 cut(s)	964, 1649
BfaI	CTAG	3 cut(s)	335, 965, 1650
BfmI	CTRYAG	1 cut(s)	570
BfoI	RGCGCY	1 cut(s)	286
BisI	GCNGC	3 cut(s)	290, 329, 762
BlpI	GCTNAGC	1 cut(s)	789
BlsI	GCNGC	3 cut(s)	291, 330, 763
Bme1390I	CCNGG	2 cut(s)	1117, 1398
Bme18I	GGWCC	1 cut(s)	1119
BmgBI	CACGTC	1 cut(s)	685
BmgT120I	GGNCC	3 cut(s)	638, 770, 1119
BmiI	GGNNCC	1 cut(s)	923
BmrFI	CCNGG	2 cut(s)	1117, 1398
BmsI	GCATC	3 cut(s)	1115, 1258, 1483
BpiI	GAAGAC	3 cut(s)	115, 306, 355
BplI	GAGNNNNNCTC	2 cut(s)	522, 554
BpmI	CTGGAG	3 cut(s)	246, 524, 1419
Bpu10I	CCTNAGC	1 cut(s)	61
Bpu1102I	GCTNAGC	1 cut(s)	789
Bpu14I	TTCGAA	1 cut(s)	1246
BpuEI	CTTGAG	2 cut(s)	506, 847
BsaAI	YACGTR	1 cut(s)	1358
BsaBI	GATNNNNATC	1 cut(s)	934
BsaJI	CCNNGG	2 cut(s)	1110, 1640
BsaWI	WCCGGW	1 cut(s)	714
Bsc4I	CCNNNNNNNGG	1 cut(s)	1116
Bse1I	ACTGG	4 cut(s)	476, 773, 913, 953
Bse3DI	GCAATG	4 cut(s)	107, 209, 988, 1083
Bse8I	GATNNNNATC	1 cut(s)	934
BseAI	TCCGGA	1 cut(s)	714
BseBI	CCWGG	2 cut(s)	1117, 1398
BseDI	CCNNGG	2 cut(s)	1110, 1640
BseGI	GGATG	6 cut(s)	442, 487, 561, 583, 592, 1106
BseJI	GATNNNNATC	1 cut(s)	934
BseLI	CCNNNNNNNGG	1 cut(s)	1116
BseMI	GCAATG	4 cut(s)	107, 209, 988, 1083
BseMII	CTCAG	2 cut(s)	75, 1143
BseNI	ACTGG	4 cut(s)	476, 773, 913, 953
BseRI	GAGGAG	2 cut(s)	31, 848
BseXI	GCAGC	2 cut(s)	340, 773
BsgI	GTGCAG	2 cut(s)	347, 945
BshFI	GGCC	3 cut(s)	639, 772, 1115
BsiHKAI	GWGCWC	3 cut(s)	62, 892, 899
BsiSI	CCGG	1 cut(s)	715
BslI	CCNNNNNNNGG	1 cut(s)	1116
BsmAI	GTCTC	3 cut(s)	542, 646, 1395
BsmI	GAATGC	2 cut(s)	438, 1369
BsnI	GGCC	3 cut(s)	639, 772, 1115
Bsp119I	TTCGAA	1 cut(s)	1246
Bsp1286I	GDGCHC	3 cut(s)	62, 892, 899
Bsp13I	TCCGGA	1 cut(s)	714
Bsp143I	GATC	5 cut(s)	193, 229, 670, 1210, 1564
Bsp1720I	GCTNAGC	1 cut(s)	789
BspACI	CCGC	5 cut(s)	289, 446, 567, 1271, 1540
BspANI	GGCC	3 cut(s)	639, 772, 1115
BspCNI	CTCAG	2 cut(s)	74, 1142
BspEI	TCCGGA	1 cut(s)	714
BspLI	GGNNCC	1 cut(s)	923
BspPI	GGATC	4 cut(s)	201, 224, 1218, 1559
BspT104I	TTCGAA	1 cut(s)	1246
BsrDI	GCAATG	4 cut(s)	107, 209, 988, 1083
BsrI	ACTGG	4 cut(s)	476, 773, 913, 953
BssECI	CCNNGG	2 cut(s)	1110, 1640
BssMI	GATC	5 cut(s)	193, 229, 670, 1210, 1564
BssT1I	CCWWGG	1 cut(s)	1640
Bst2UI	CCWGG	2 cut(s)	1117, 1398
Bst4CI	ACNGT	2 cut(s)	325, 1262
Bst6I	CTCTTC	1 cut(s)	1325
BstBAI	YACGTR	1 cut(s)	1358
BstBI	TTCGAA	1 cut(s)	1246
BstC8I	GCNNGC	6 cut(s)	287, 387, 786, 823, 1074, 1369
BstDEI	CTNAG	3 cut(s)	61, 789, 1129
BstF5I	GGATG	6 cut(s)	442, 487, 561, 583, 592, 1106
BstH2I	RGCGCY	1 cut(s)	286
BstHHI	GCGC	1 cut(s)	285
BstKTI	GATC	5 cut(s)	196, 232, 673, 1213, 1567
BstMAI	GTCTC	3 cut(s)	542, 646, 1395
BstMBI	GATC	5 cut(s)	193, 229, 670, 1210, 1564
BstMWI	GCNNNNNNNGC	3 cut(s)	289, 790, 1112
BstNI	CCWGG	2 cut(s)	1117, 1398
BstNSI	RCATGY	2 cut(s)	564, 1352
BstSCI	CCNGG	2 cut(s)	1115, 1396
BstSFI	CTRYAG	1 cut(s)	570
BstV1I	GCAGC	2 cut(s)	340, 773
BstV2I	GAAGAC	3 cut(s)	115, 306, 355
BstX2I	RGATCY	2 cut(s)	229, 1564
BstYI	RGATCY	2 cut(s)	229, 1564
BsuRI	GGCC	3 cut(s)	639, 772, 1115
BtrI	CACGTC	1 cut(s)	685
BtsCI	GGATG	6 cut(s)	442, 487, 561, 583, 592, 1106
BtsI	GCAGTG	2 cut(s)	800, 1183
BtsIMutI	CAGTG	5 cut(s)	308, 800, 892, 906, 1183
Cac8I	GCNNGC	6 cut(s)	287, 387, 786, 823, 1074, 1369
CfoI	GCGC	1 cut(s)	285
Cfr13I	GGNCC	3 cut(s)	638, 770, 1119
Csp6I	GTAC	3 cut(s)	564, 1346, 1450
CviAII	CATG	6 cut(s)	561, 629, 733, 1349, 1372, 1405
CviQI	GTAC	3 cut(s)	564, 1346, 1450
DdeI	CTNAG	3 cut(s)	61, 789, 1129
DpnI	GATC	5 cut(s)	195, 231, 672, 1212, 1566
DpnII	GATC	5 cut(s)	193, 229, 670, 1210, 1564
DraI	TTTAAA	1 cut(s)	72
DraIII	CACNNNGTG	1 cut(s)	190
Eam1104I	CTCTTC	1 cut(s)	1325
EarI	CTCTTC	1 cut(s)	1325
Ecl136II	GAGCTC	1 cut(s)	60
Eco130I	CCWWGG	1 cut(s)	1640
Eco147I	AGGCCT	1 cut(s)	1115
Eco24I	GRGCYC	1 cut(s)	62
Eco47I	GGWCC	1 cut(s)	1119
Eco47III	AGCGCT	1 cut(s)	284
Eco53kI	GAGCTC	1 cut(s)	60
Eco57I	CTGAAG	2 cut(s)	131, 332
Eco72I	CACGTG	1 cut(s)	1358
EcoICRI	GAGCTC	1 cut(s)	60
EcoRII	CCWGG	2 cut(s)	1115, 1396
EcoT14I	CCWWGG	1 cut(s)	1640
EcoT22I	ATGCAT	1 cut(s)	610
EcoT38I	GRGCYC	1 cut(s)	62
ErhI	CCWWGG	1 cut(s)	1640
FaeI	CATG	6 cut(s)	564, 632, 736, 1352, 1375, 1408
FatI	CATG	6 cut(s)	560, 628, 732, 1348, 1371, 1404
FauI	CCCGC	1 cut(s)	1533
FauNDI	CATATG	1 cut(s)	766
FbaI	TGATCA	1 cut(s)	670
Fnu4HI	GCNGC	3 cut(s)	290, 329, 762
FokI	GGATG	6 cut(s)	429, 494, 568, 590, 599, 1093
FriOI	GRGCYC	1 cut(s)	62
Fsp4HI	GCNGC	3 cut(s)	290, 329, 762
FspBI	CTAG	3 cut(s)	335, 965, 1650
GlaI	GCGC	1 cut(s)	284
GluI	GCNGC	3 cut(s)	290, 329, 762
GsuI	CTGGAG	3 cut(s)	246, 524, 1419
HaeII	RGCGCY	1 cut(s)	286
HaeIII	GGCC	3 cut(s)	639, 772, 1115
HapII	CCGG	1 cut(s)	715
HhaI	GCGC	1 cut(s)	285
Hin1II	CATG	6 cut(s)	564, 632, 736, 1352, 1375, 1408
Hin6I	GCGC	1 cut(s)	283
HinP1I	GCGC	1 cut(s)	283
HincII	GTYRAC	3 cut(s)	722, 973, 1191
HindII	GTYRAC	3 cut(s)	722, 973, 1191
HinfI	GANTC	7 cut(s)	338, 535, 718, 903, 991, 1125, 1478
HpaII	CCGG	1 cut(s)	715
HphI	GGTGA	1 cut(s)	1502
Hpy166II	GTNNAC	5 cut(s)	426, 722, 973, 1135, 1191
Hpy188I	TCNGA	7 cut(s)	57, 136, 940, 1111, 1207, 1330, 1420
Hpy188III	TCNNGA	8 cut(s)	63, 296, 465, 523, 541, 600, 715, 900
Hpy8I	GTNNAC	5 cut(s)	426, 722, 973, 1135, 1191
HpyAV	CCTTC	7 cut(s)	307, 391, 441, 452, 523, 527, 1471
HpyCH4III	ACNGT	2 cut(s)	325, 1262
HpyCH4IV	ACGT	4 cut(s)	684, 861, 1357, 1508
HpyF10VI	GCNNNNNNNGC	3 cut(s)	289, 790, 1112
HpyF3I	CTNAG	3 cut(s)	61, 789, 1129
HpySE526I	ACGT	4 cut(s)	684, 861, 1357, 1508
Hsp92II	CATG	6 cut(s)	564, 632, 736, 1352, 1375, 1408
HspAI	GCGC	1 cut(s)	283
Kpn2I	TCCGGA	1 cut(s)	714
Ksp22I	TGATCA	1 cut(s)	670
Kzo9I	GATC	5 cut(s)	193, 229, 670, 1210, 1564
LmnI	GCTCC	2 cut(s)	57, 895
Lsp1109I	GCAGC	2 cut(s)	340, 773
LweI	GCATC	3 cut(s)	1115, 1258, 1483
MaeI	CTAG	3 cut(s)	335, 965, 1650
MaeII	ACGT	4 cut(s)	684, 861, 1357, 1508
MaeIII	GTNAC	1 cut(s)	1262
MalI	GATC	5 cut(s)	195, 231, 672, 1212, 1566
MboI	GATC	5 cut(s)	193, 229, 670, 1210, 1564
MfeI	CAATTG	2 cut(s)	492, 840
MflI	RGATCY	2 cut(s)	229, 1564
MhlI	GDGCHC	3 cut(s)	62, 892, 899
MlyI	GAGTC	3 cut(s)	544, 727, 985
MmeI	TCCRAC	2 cut(s)	35, 1592
Mph1103I	ATGCAT	1 cut(s)	610
MroI	TCCGGA	1 cut(s)	714
MroXI	GAANNNNTTC	1 cut(s)	303
MseI	TTAA	8 cut(s)	27, 71, 122, 738, 1148, 1334, 1580, 1654
MslI	CAYNNNNRTG	1 cut(s)	100
MspA1I	CMGCKG	1 cut(s)	448
MspI	CCGG	1 cut(s)	715
MspR9I	CCNGG	2 cut(s)	1117, 1398
MunI	CAATTG	2 cut(s)	492, 840
Mva1269I	GAATGC	2 cut(s)	438, 1369
MvaI	CCWGG	2 cut(s)	1117, 1398
MwoI	GCNNNNNNNGC	3 cut(s)	289, 790, 1112
NdeI	CATATG	1 cut(s)	766
NdeII	GATC	5 cut(s)	193, 229, 670, 1210, 1564
NlaIII	CATG	6 cut(s)	564, 632, 736, 1352, 1375, 1408
NlaIV	GGNNCC	1 cut(s)	923
NsiI	ATGCAT	1 cut(s)	610
NspI	RCATGY	2 cut(s)	564, 1352
NspV	TTCGAA	1 cut(s)	1246
PceI	AGGCCT	1 cut(s)	1115
PciI	ACATGT	2 cut(s)	560, 1348
PctI	GAATGC	2 cut(s)	438, 1369
PdmI	GAANNNNTTC	1 cut(s)	303
PfeI	GAWTC	4 cut(s)	338, 903, 1125, 1478
PkrI	GCNGC	3 cut(s)	291, 330, 763
PleI	GAGTC	3 cut(s)	543, 726, 985
PmaCI	CACGTG	1 cut(s)	1358
PmlI	CACGTG	1 cut(s)	1358
PpsI	GAGTC	3 cut(s)	543, 726, 985
Ppu21I	YACGTR	1 cut(s)	1358
PscI	ACATGT	2 cut(s)	560, 1348
PshBI	ATTAAT	1 cut(s)	738
Psp124BI	GAGCTC	1 cut(s)	62
Psp6I	CCWGG	2 cut(s)	1115, 1396
PspCI	CACGTG	1 cut(s)	1358
PspGI	CCWGG	2 cut(s)	1115, 1396
PspN4I	GGNNCC	1 cut(s)	923
PspPI	GGNCC	3 cut(s)	638, 770, 1119
PsuI	RGATCY	2 cut(s)	229, 1564
RsaI	GTAC	3 cut(s)	565, 1347, 1451
RsaNI	GTAC	3 cut(s)	564, 1346, 1450
RseI	CAYNNNNRTG	1 cut(s)	100
SacI	GAGCTC	1 cut(s)	62
SaqAI	TTAA	8 cut(s)	27, 71, 122, 738, 1148, 1334, 1580, 1654
SatI	GCNGC	3 cut(s)	290, 329, 762
Sau3AI	GATC	5 cut(s)	193, 229, 670, 1210, 1564
Sau96I	GGNCC	3 cut(s)	638, 770, 1119
SchI	GAGTC	3 cut(s)	544, 727, 985
ScrFI	CCNGG	2 cut(s)	1117, 1398
SduI	GDGCHC	3 cut(s)	62, 892, 899
SfaNI	GCATC	3 cut(s)	1115, 1258, 1483
SfcI	CTRYAG	1 cut(s)	570
SfuI	TTCGAA	1 cut(s)	1246
SinI	GGWCC	1 cut(s)	1119
SmiMI	CAYNNNNRTG	1 cut(s)	100
SmlI	CTYRAG	2 cut(s)	521, 826
SmoI	CTYRAG	2 cut(s)	521, 826
SpeI	ACTAGT	2 cut(s)	964, 1649
SseBI	AGGCCT	1 cut(s)	1115
SsiI	CCGC	5 cut(s)	289, 446, 567, 1271, 1540
SspMI	CTAG	3 cut(s)	335, 965, 1650
SstI	GAGCTC	1 cut(s)	62
StuI	AGGCCT	1 cut(s)	1115
StyD4I	CCNGG	2 cut(s)	1115, 1396
StyI	CCWWGG	1 cut(s)	1640
TaaI	ACNGT	2 cut(s)	325, 1262
TaiI	ACGT	4 cut(s)	687, 864, 1360, 1511
TaqI	TCGA	1 cut(s)	1246
TauI	GCSGC	1 cut(s)	292
TfiI	GAWTC	4 cut(s)	338, 903, 1125, 1478
Tru1I	TTAA	8 cut(s)	27, 71, 122, 738, 1148, 1334, 1580, 1654
Tru9I	TTAA	8 cut(s)	27, 71, 122, 738, 1148, 1334, 1580, 1654
TscAI	CASTG	5 cut(s)	315, 800, 892, 913, 1183
TseI	GCWGC	2 cut(s)	328, 761
TspDTI	ATGAA	5 cut(s)	345, 431, 1019, 1062, 1421
TspGWI	ACGGA	1 cut(s)	882
TspRI	CASTG	5 cut(s)	315, 800, 892, 913, 1183
VpaK11BI	GGWCC	1 cut(s)	1119
VspI	ATTAAT	1 cut(s)	738
XapI	RAATTY	5 cut(s)	250, 270, 621, 952, 1021
XceI	RCATGY	2 cut(s)	564, 1352
XmnI	GAANNNNTTC	1 cut(s)	303
XspI	CTAG	3 cut(s)	335, 965, 1650
Zsp2I	ATGCAT	1 cut(s)	610

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.