FvH4_1g14550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009464
Physical Location & Seq
Forward (+)
8112131 .. 8114277
2147 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g14550.t1

Sequence Viewer

Length: 546 bp
ATGTCGGACAGAGAAGATTACGACTCGGACGCGCCGGAGGAGTTCACAGCCGAGCAGGGAATACAGCAAGAACAAGAACTCAGTAAGCTTCAGAAAGAGAATAAGGCCAGGGTTGCTCGTGAAGGAAAAGAACGTCGTAGAAAATGGGCCCAGAAGAAAACACCACGGAAAGCTACTACCCAGGTTGAAAGTGTTCAAGATGCTTCTGAAAATGAAACACTTGAAGAATCTTCTATGGGCACTGCTGGGATGCTTCCTTCTAACATTGTTGAACTGCTTGCAGCTCGTGAGAAGCAAGTTTTTTCAGACTCTGATGATGAGAAGGATGACTTGAAGCCTACTAAGAAGAAAAAACGAAAAAGCTCAGGGGTTGAAACTATTATTCTGAATCAGACAGCTCCTCCTCCATGCTTAGAGAAGTCCTTGGAATTCTTAAAGCAAAGGAAAATGCAGGTCCCCAGATCACAAGCAGTATTAAATAACTCAAGCCAAGCATTACGCTTTCTCACTTCTTCGGGCGTGTTGAGTAAAAAGCTGACAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.44

Weight (kDa)

8.89

Isoelectric Point (pI)

58.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 442
AccII CGCG 1 cut(s) 32
AcsI RAATTY 1 cut(s) 428
AcuI CTGAAG 1 cut(s) 74
AgsI TTSAA 6 cut(s) 188, 197, 224, 272, 334, 374
AjnI CCWGG 2 cut(s) 107, 180
AluBI AGCT 6 cut(s) 88, 173, 284, 363, 398, 535
AluI AGCT 6 cut(s) 88, 173, 284, 363, 398, 535
AlwNI CAGNNNCTG 1 cut(s) 311
AoxI GGCC 2 cut(s) 105, 147
ApaI GGGCCC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 281
ApoI RAATTY 1 cut(s) 428
Asp700I GAANNNNTTC 1 cut(s) 192
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 3 cut(s) 147, 148, 454
AvaII GGWCC 1 cut(s) 454
BaeGI GKGCMC 2 cut(s) 151, 242
BanII GRGCYC 1 cut(s) 151
BauI CACGAG 2 cut(s) 117, 285
BbvI GCAGC 1 cut(s) 293
BciT130I CCWGG 2 cut(s) 109, 182
BfuAI ACCTGC 1 cut(s) 442
BisI GCNGC 1 cut(s) 282
BlsI GCNGC 1 cut(s) 283
Bme1390I CCNGG 2 cut(s) 109, 182
Bme18I GGWCC 1 cut(s) 454
BmgT120I GGNCC 3 cut(s) 147, 148, 454
BmiI GGNNCC 2 cut(s) 149, 456
BmrFI CCNGG 2 cut(s) 109, 182
BmsI GCATC 2 cut(s) 190, 240
Bpu10I CCTNAGC 1 cut(s) 364
BpuEI CTTGAG 1 cut(s) 469
BsaJI CCNNGG 4 cut(s) 108, 164, 180, 423
BsaXI ACNNNNNCTCC 2 cut(s) 385, 415
BseBI CCWGG 2 cut(s) 109, 182
BseDI CCNNGG 4 cut(s) 108, 164, 180, 423
BseGI GGATG 2 cut(s) 255, 331
BseMII CTCAG 2 cut(s) 94, 378
BseRI GAGGAG 3 cut(s) 53, 390, 393
BseSI GKGCMC 2 cut(s) 151, 242
BseXI GCAGC 1 cut(s) 293
BseYI CCCAGC 1 cut(s) 245
Bsh1236I CGCG 1 cut(s) 32
BshFI GGCC 2 cut(s) 107, 149
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 440
BsmFI GGGAC 1 cut(s) 440
BsnI GGCC 2 cut(s) 107, 149
Bsp120I GGGCCC 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 151, 242
Bsp143I GATC 1 cut(s) 461
BspANI GGCC 2 cut(s) 107, 149
BspCNI CTCAG 2 cut(s) 93, 377
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 2 cut(s) 149, 456
BspMI ACCTGC 1 cut(s) 442
BssECI CCNNGG 4 cut(s) 108, 164, 180, 423
BssMI GATC 1 cut(s) 461
BssSI CACGAG 2 cut(s) 117, 285
BssT1I CCWWGG 1 cut(s) 423
Bst2BI CACGAG 2 cut(s) 117, 285
Bst2UI CCWGG 2 cut(s) 109, 182
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 4 cut(s) 80, 342, 364, 412
BstDSI CCRYGG 1 cut(s) 164
BstF5I GGATG 2 cut(s) 255, 331
BstFNI CGCG 1 cut(s) 32
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 1 cut(s) 464
BstMBI GATC 1 cut(s) 461
BstMWI GCNNNNNNNGC 1 cut(s) 113
BstNI CCWGG 2 cut(s) 109, 182
BstSCI CCNGG 2 cut(s) 107, 180
BstSLI GKGCMC 2 cut(s) 151, 242
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 293
BsuRI GGCC 2 cut(s) 107, 149
BtgI CCRYGG 1 cut(s) 164
BtsCI GGATG 2 cut(s) 255, 331
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 1 cut(s) 240
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 279
CaiI CAGNNNCTG 1 cut(s) 311
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 147, 148, 454
CseI GACGC 1 cut(s) 38
CviAII CATG 1 cut(s) 408
DdeI CTNAG 4 cut(s) 80, 342, 364, 412
DpnI GATC 1 cut(s) 463
DpnII GATC 1 cut(s) 461
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 74
EcoO109I RGGNCCY 1 cut(s) 454
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 2 cut(s) 107, 180
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 1 cut(s) 411
FaiI YATR 2 cut(s) 236, 409
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 440
FatI CATG 1 cut(s) 407
Fnu4HI GCNGC 1 cut(s) 282
FokI GGATG 2 cut(s) 262, 338
FriOI GRGCYC 1 cut(s) 151
Fsp4HI GCNGC 1 cut(s) 282
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 282
GsaI CCCAGC 1 cut(s) 249
HaeIII GGCC 2 cut(s) 107, 149
HapII CCGG 1 cut(s) 35
HgaI GACGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 34
Hin1II CATG 1 cut(s) 411
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 4 cut(s) 23, 227, 308, 388
HpaII CCGG 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 8 cut(s) 7, 28, 93, 208, 307, 313, 387, 393
Hpy188III TCNNGA 3 cut(s) 119, 197, 287
Hpy8I GTNNAC 1 cut(s) 45
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 3 cut(s) 116, 267, 316
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 2 cut(s) 281, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 113
HpyF3I CTNAG 4 cut(s) 80, 342, 364, 412
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 1 cut(s) 411
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 1 cut(s) 461
LmnI GCTCC 1 cut(s) 403
Lsp1109I GCAGC 1 cut(s) 293
LweI GCATC 2 cut(s) 190, 240
MaeII ACGT 1 cut(s) 133
MalI GATC 1 cut(s) 463
MboI GATC 1 cut(s) 461
MboII GAAGA 6 cut(s) 26, 166, 222, 236, 358, 504
MhlI GDGCHC 2 cut(s) 151, 242
MluCI AATT 1 cut(s) 428
MlyI GAGTC 2 cut(s) 17, 302
MnlI CCTC 3 cut(s) 31, 411, 414
MroXI GAANNNNTTC 1 cut(s) 192
MseI TTAA 2 cut(s) 434, 476
MspI CCGG 1 cut(s) 35
MspR9I CCNGG 2 cut(s) 109, 182
MvaI CCWGG 2 cut(s) 109, 182
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 1 cut(s) 113
NdeII GATC 1 cut(s) 461
NlaIII CATG 1 cut(s) 411
NlaIV GGNNCC 2 cut(s) 149, 456
NmeAIII GCCGAG 1 cut(s) 76
PdmI GAANNNNTTC 1 cut(s) 192
PfeI GAWTC 2 cut(s) 227, 388
PkrI GCNGC 1 cut(s) 283
PleI GAGTC 2 cut(s) 17, 302
PpsI GAGTC 2 cut(s) 17, 302
PpuMI RGGWCCY 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 454
Psp6I CCWGG 2 cut(s) 107, 180
PspFI CCCAGC 1 cut(s) 245
PspGI CCWGG 2 cut(s) 107, 180
PspN4I GGNNCC 2 cut(s) 149, 456
PspOMI GGGCCC 1 cut(s) 147
PspPI GGNCC 3 cut(s) 147, 148, 454
PspPPI RGGWCCY 1 cut(s) 454
PstNI CAGNNNCTG 1 cut(s) 311
SaqAI TTAA 2 cut(s) 434, 476
SatI GCNGC 1 cut(s) 282
Sau3AI GATC 1 cut(s) 461
Sau96I GGNCC 3 cut(s) 147, 148, 454
SchI GAGTC 2 cut(s) 17, 302
ScrFI CCNGG 2 cut(s) 109, 182
SduI GDGCHC 2 cut(s) 151, 242
SetI ASST 9 cut(s) 90, 136, 175, 186, 286, 365, 400, 456, 537
SfaNI GCATC 2 cut(s) 190, 240
SinI GGWCC 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
Sse9I AATT 1 cut(s) 428
StyD4I CCNGG 2 cut(s) 107, 180
StyI CCWWGG 1 cut(s) 423
TaiI ACGT 1 cut(s) 136
TasI AATT 1 cut(s) 428
TfiI GAWTC 2 cut(s) 227, 388
Tru1I TTAA 2 cut(s) 434, 476
Tru9I TTAA 2 cut(s) 434, 476
TscAI CASTG 1 cut(s) 247
TseI GCWGC 1 cut(s) 281
TspDTI ATGAA 1 cut(s) 228
TspGWI ACGGA 1 cut(s) 181
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 454
XapI RAATTY 1 cut(s) 428
XmnI GAANNNNTTC 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.