Prupe.7G145000_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0009464
Physical Location & Seq
Reverse (-)
15863522 .. 15865572
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G145000.2

Sequence Viewer

Length: 558 bp
ATGTCGGACAGAGAAGATTACGATTCGGACGCCCCCGAGGAGCTCACAGCCGAGCAGGGAATACAACAAGATGAAGAACTAAGTAAGGTCCAGAAAGAGAACAAAGCGAGGGTGGTTCGTGAAGGAAAAGAACGTCGTAGACAATGGGCCCAAAGGAAAACACCAAAGAAAACACCACAGCCGTCTGCTGGAGTTGAAAGTGTTCAAGATGCCCCTGAAACTGAAACACACCAAGAATCTTTGGGTAGTGCAGGGATGCTTCCAAGCAACATTGTTGAATTGCTTGCAGCTCGTGAGAAACAAGTGTTCCACTCAGACTCTGAGGATGAGAATACTGACTCAAAGTCCACTAAGAAAAAGAAAAGGCTAAAAAGCTCAGGGGTTGATACTGTTATTTTGAAGGAGACACCTGCTCCAAAATGCTTACAGAATTCCTTGGCGTTCTTGGAGAAAAGGAAAATGCAGGTGTCTAGATCACATGCAGTGTTGAACAACTCCAACCAAGCCTTACGCTTCCTCTCTTCTTCTGGCTTGTTGAGTAAAAACGTGACGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

20.66

Weight (kDa)

8.59

Isoelectric Point (pI)

63.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 418, 454
Acc36I ACCTGC 2 cut(s) 418, 454
AccI GTMKAC 1 cut(s) 139
AcsI RAATTY 1 cut(s) 430
AcyI GRCGYC 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 188
AgsI TTSAA 5 cut(s) 197, 206, 278, 400, 490
AhdI GACNNNNNGTC 1 cut(s) 343
AluBI AGCT 4 cut(s) 43, 290, 375, 555
AluI AGCT 4 cut(s) 43, 290, 375, 555
Alw21I GWGCWC 1 cut(s) 45
Alw26I GTCTC 1 cut(s) 398
AlwNI CAGNNNCTG 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 35
AoxI GGCC 1 cut(s) 147
ApaI GGGCCC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 430
Asp700I GAANNNNTTC 1 cut(s) 201
AspS9I GGNCC 3 cut(s) 88, 147, 148
AvaI CYCGRG 1 cut(s) 35
AvaII GGWCC 1 cut(s) 88
BaeGI GKGCMC 1 cut(s) 151
BanII GRGCYC 2 cut(s) 45, 151
BauI CACGAG 1 cut(s) 291
Bbv12I GWGCWC 1 cut(s) 45
BbvI GCAGC 1 cut(s) 299
BceAI ACGGC 1 cut(s) 166
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 1 cut(s) 471
BfuAI ACCTGC 2 cut(s) 418, 454
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme18I GGWCC 1 cut(s) 88
BmeRI GACNNNNNGTC 1 cut(s) 343
BmeT110I CYCGRG 1 cut(s) 35
BmgT120I GGNCC 3 cut(s) 88, 147, 148
BmiI GGNNCC 1 cut(s) 149
BmsI GCATC 2 cut(s) 199, 246
BpmI CTGGAG 1 cut(s) 210
Bpu10I CCTNAGC 1 cut(s) 376
BsaHI GRCGYC 1 cut(s) 30
BsaJI CCNNGG 2 cut(s) 36, 435
BsaXI ACNNNNNCTCC 2 cut(s) 397, 427
Bsc4I CCNNNNNNNGG 1 cut(s) 188
BseDI CCNNGG 2 cut(s) 36, 435
BseGI GGATG 2 cut(s) 261, 331
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMII CTCAG 3 cut(s) 312, 327, 390
BseRI GAGGAG 1 cut(s) 53
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 1 cut(s) 299
BsgI GTGCAG 1 cut(s) 270
BshFI GGCC 1 cut(s) 149
BsiHKAI GWGCWC 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 35
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 1 cut(s) 398
BsnI GGCC 1 cut(s) 149
BsoBI CYCGRG 1 cut(s) 35
Bsp120I GGGCCC 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 45, 151
Bsp143I GATC 1 cut(s) 473
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 3 cut(s) 313, 326, 389
BspLI GGNNCC 1 cut(s) 149
BspMI ACCTGC 2 cut(s) 418, 454
BssECI CCNNGG 2 cut(s) 36, 435
BssMI GATC 1 cut(s) 473
BssNI GRCGYC 1 cut(s) 30
BssSI CACGAG 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 435
Bst2BI CACGAG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 1 cut(s) 526
BstACI GRCGYC 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 5 cut(s) 80, 313, 321, 351, 376
BstF5I GGATG 2 cut(s) 261, 331
BstKTI GATC 1 cut(s) 476
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 1 cut(s) 473
BstNSI RCATGY 1 cut(s) 482
BstSLI GKGCMC 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 299
BsuRI GGCC 1 cut(s) 149
BtsCI GGATG 2 cut(s) 261, 331
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 1 cut(s) 489
BveI ACCTGC 2 cut(s) 418, 454
Cac8I GCNNGC 1 cut(s) 285
CaiI CAGNNNCTG 1 cut(s) 320
Cfr13I GGNCC 3 cut(s) 88, 147, 148
CseI GACGC 1 cut(s) 38
CviAII CATG 1 cut(s) 479
DdeI CTNAG 5 cut(s) 80, 313, 321, 351, 376
DpnI GATC 1 cut(s) 475
DpnII GATC 1 cut(s) 473
DriI GACNNNNNGTC 1 cut(s) 343
Eam1104I CTCTTC 1 cut(s) 526
Eam1105I GACNNNNNGTC 1 cut(s) 343
EarI CTCTTC 1 cut(s) 526
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 435
Eco24I GRGCYC 2 cut(s) 45, 151
Eco47I GGWCC 1 cut(s) 88
Eco53kI GAGCTC 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 35
EcoICRI GAGCTC 1 cut(s) 43
EcoRI GAATTC 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 435
EcoT38I GRGCYC 2 cut(s) 45, 151
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 1 cut(s) 482
FaiI YATR 1 cut(s) 480
FatI CATG 1 cut(s) 478
FblI GTMKAC 1 cut(s) 139
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 2 cut(s) 268, 338
FriOI GRGCYC 2 cut(s) 45, 151
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 1 cut(s) 471
GluI GCNGC 1 cut(s) 288
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 1 cut(s) 149
HgaI GACGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 1 cut(s) 482
HinfI GANTC 4 cut(s) 23, 236, 317, 338
Hpy166II GTNNAC 2 cut(s) 140, 348
Hpy188I TCNGA 4 cut(s) 7, 28, 316, 322
Hpy188III TCNNGA 5 cut(s) 91, 119, 206, 293, 471
Hpy8I GTNNAC 2 cut(s) 140, 348
Hpy99I CGWCG 1 cut(s) 138
HpyAV CCTTC 2 cut(s) 116, 394
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 2 cut(s) 133, 546
HpyCH4V TGCA 4 cut(s) 251, 287, 463, 482
HpyF3I CTNAG 5 cut(s) 80, 313, 321, 351, 376
HpySE526I ACGT 2 cut(s) 133, 546
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 1 cut(s) 482
Kzo9I GATC 1 cut(s) 473
LmnI GCTCC 2 cut(s) 40, 418
LpnPI CCDG 9 cut(s) 41, 104, 174, 228, 237, 363, 423, 449, 513
Lsp1109I GCAGC 1 cut(s) 299
LweI GCATC 2 cut(s) 199, 246
MaeI CTAG 1 cut(s) 471
MaeII ACGT 2 cut(s) 133, 546
MaeIII GTNAC 1 cut(s) 547
MalI GATC 1 cut(s) 475
MboI GATC 1 cut(s) 473
MboII GAAGA 4 cut(s) 26, 86, 513, 516
MhlI GDGCHC 2 cut(s) 45, 151
MluCI AATT 2 cut(s) 278, 430
MlyI GAGTC 2 cut(s) 311, 332
MmeI TCCRAC 1 cut(s) 522
MnlI CCTC 4 cut(s) 31, 102, 316, 527
MroXI GAANNNNTTC 1 cut(s) 201
NdeII GATC 1 cut(s) 473
NlaIII CATG 1 cut(s) 482
NlaIV GGNNCC 1 cut(s) 149
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 547
NspI RCATGY 1 cut(s) 482
PaqCI CACCTGC 2 cut(s) 418, 454
PdmI GAANNNNTTC 1 cut(s) 201
PfeI GAWTC 2 cut(s) 23, 236
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 2 cut(s) 311, 332
PpsI GAGTC 2 cut(s) 311, 332
Psp124BI GAGCTC 1 cut(s) 45
PspN4I GGNNCC 1 cut(s) 149
PspOMI GGGCCC 1 cut(s) 147
PspPI GGNCC 3 cut(s) 88, 147, 148
PstNI CAGNNNCTG 1 cut(s) 320
SacI GAGCTC 1 cut(s) 45
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 1 cut(s) 473
Sau96I GGNCC 3 cut(s) 88, 147, 148
SchI GAGTC 2 cut(s) 311, 332
SduI GDGCHC 2 cut(s) 45, 151
SetI ASST 9 cut(s) 45, 90, 136, 292, 377, 412, 468, 549, 557
SfaNI GCATC 2 cut(s) 199, 246
SinI GGWCC 1 cut(s) 88
Sse9I AATT 2 cut(s) 278, 430
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 1 cut(s) 45
StyI CCWWGG 1 cut(s) 435
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 2 cut(s) 136, 549
TasI AATT 2 cut(s) 278, 430
TfiI GAWTC 2 cut(s) 23, 236
TscAI CASTG 1 cut(s) 489
TseFI GTSAC 1 cut(s) 547
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 1 cut(s) 547
TspDTI ATGAA 1 cut(s) 87
TspRI CASTG 1 cut(s) 489
VpaK11BI GGWCC 1 cut(s) 88
XapI RAATTY 1 cut(s) 430
XbaI TCTAGA 1 cut(s) 470
XceI RCATGY 1 cut(s) 482
XmiI GTMKAC 1 cut(s) 139
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.