Rroxscaffold_2G00139760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009464
Physical Location & Seq
Reverse (-)
78111155 .. 78113067
1913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00139760.1

Sequence Viewer

Length: 543 bp
ATGTCGGACAGAGAAGATTACGACTCCGACGCGCCGGAGGAGTTCACATCCGAGCAGGGAATACAACAGGAACAAGAGCTGAGTAAGCTTCAGAAAGAGAATAAGGCGAGGGTTGCTCGTGAAGGAAAAGAACGTCGTAGAAAATGGGCCCAGAAAAAAACACCACGGAAAGATACTGTTGAAAGTGTTCAAGATGTTTCTGAAAATGAAACACACCAAGAATCTTCCATGGGCACTGCAGGGATGCTTCCTCCTAACATTGTTGAACTACTTGCAGCTCGTGAGAAGCAAGTTTTCTCAGACTCTGAGGATGAGAAGGCTGACTCGAAATCTACTAAGAAGAAGAAAAAACGAAAAACCTCAGGGGTTGATACTATTATTCTGAATCAGACAGCTCCTCCTCCATGCTTAGAGAAGTCCTTGGAATTCTTGAAGAAAAGGAAAATGCAGGTCCCCAGATCACAAGCAGTGTTGAACAACTCGAACCAAGCATTACGATTTCTCACTTCTTCAGGCGTGTTAAGTAAAAAACTGACAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

20.42

Weight (kDa)

9.34

Isoelectric Point (pI)

57.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 439
AccII CGCG 1 cut(s) 32
AcsI RAATTY 1 cut(s) 425
AcuI CTGAAG 2 cut(s) 74, 495
AgsI TTSAA 5 cut(s) 182, 191, 266, 433, 475
AluBI AGCT 4 cut(s) 79, 88, 278, 395
AluI AGCT 4 cut(s) 79, 88, 278, 395
AlwNI CAGNNNCTG 1 cut(s) 305
AoxI GGCC 1 cut(s) 147
ApaI GGGCCC 1 cut(s) 151
ApeKI GCWGC 1 cut(s) 275
ApoI RAATTY 1 cut(s) 425
Asp700I GAANNNNTTC 1 cut(s) 186
AspLEI GCGC 1 cut(s) 34
AspS9I GGNCC 3 cut(s) 147, 148, 451
AvaII GGWCC 1 cut(s) 451
AxyI CCTNAGG 1 cut(s) 361
BaeGI GKGCMC 2 cut(s) 151, 236
BanII GRGCYC 1 cut(s) 151
BauI CACGAG 2 cut(s) 117, 279
BbvI GCAGC 1 cut(s) 287
BfmI CTRYAG 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 439
BisI GCNGC 1 cut(s) 276
BlsI GCNGC 1 cut(s) 277
Bme18I GGWCC 1 cut(s) 451
BmgT120I GGNCC 3 cut(s) 147, 148, 451
BmiI GGNNCC 2 cut(s) 149, 453
BmsI GCATC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 100, 132
BsaJI CCNNGG 3 cut(s) 164, 228, 420
BsaXI ACNNNNNCTCC 2 cut(s) 382, 412
Bse21I CCTNAGG 1 cut(s) 361
BseDI CCNNGG 3 cut(s) 164, 228, 420
BseGI GGATG 3 cut(s) 47, 249, 316
BseMII CTCAG 4 cut(s) 71, 297, 312, 375
BseRI GAGGAG 3 cut(s) 53, 387, 390
BseSI GKGCMC 2 cut(s) 151, 236
BseXI GCAGC 1 cut(s) 287
Bsh1236I CGCG 1 cut(s) 32
BshFI GGCC 1 cut(s) 149
BsiSI CCGG 1 cut(s) 35
BslFI GGGAC 1 cut(s) 437
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 1 cut(s) 149
Bsp120I GGGCCC 1 cut(s) 147
Bsp1286I GDGCHC 2 cut(s) 151, 236
Bsp143I GATC 1 cut(s) 458
Bsp19I CCATGG 1 cut(s) 228
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 4 cut(s) 72, 298, 311, 374
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 2 cut(s) 149, 453
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 439
BssECI CCNNGG 3 cut(s) 164, 228, 420
BssMI GATC 1 cut(s) 458
BssSI CACGAG 2 cut(s) 117, 279
BssT1I CCWWGG 2 cut(s) 228, 420
Bst2BI CACGAG 2 cut(s) 117, 279
Bst4CI ACNGT 1 cut(s) 178
BstDEI CTNAG 6 cut(s) 80, 298, 306, 336, 361, 409
BstDSI CCRYGG 2 cut(s) 164, 228
BstF5I GGATG 3 cut(s) 47, 249, 316
BstFNI CGCG 1 cut(s) 32
BstHHI GCGC 1 cut(s) 34
BstKTI GATC 1 cut(s) 461
BstMBI GATC 1 cut(s) 458
BstMWI GCNNNNNNNGC 2 cut(s) 85, 113
BstSFI CTRYAG 1 cut(s) 237
BstSLI GKGCMC 2 cut(s) 151, 236
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 287
Bsu36I CCTNAGG 1 cut(s) 361
BsuRI GGCC 1 cut(s) 149
BtgI CCRYGG 2 cut(s) 164, 228
BtsCI GGATG 3 cut(s) 47, 249, 316
BtsI GCAGTG 2 cut(s) 234, 474
BtsIMutI CAGTG 2 cut(s) 234, 474
BveI ACCTGC 1 cut(s) 439
CaiI CAGNNNCTG 1 cut(s) 305
CfoI GCGC 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 147, 148, 451
CseI GACGC 1 cut(s) 38
CviAII CATG 2 cut(s) 229, 405
CviJI RGCY 6 cut(s) 79, 88, 149, 278, 320, 395
CviKI_1 RGCY 6 cut(s) 79, 88, 149, 278, 320, 395
DdeI CTNAG 6 cut(s) 80, 298, 306, 336, 361, 409
DpnI GATC 1 cut(s) 460
DpnII GATC 1 cut(s) 458
Eco130I CCWWGG 2 cut(s) 228, 420
Eco24I GRGCYC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 451
Eco57I CTGAAG 2 cut(s) 74, 495
Eco81I CCTNAGG 1 cut(s) 361
EcoO109I RGGNCCY 1 cut(s) 451
EcoRI GAATTC 1 cut(s) 425
EcoT14I CCWWGG 2 cut(s) 228, 420
EcoT38I GRGCYC 1 cut(s) 151
ErhI CCWWGG 2 cut(s) 228, 420
FaeI CATG 2 cut(s) 232, 408
FaiI YATR 2 cut(s) 230, 406
FaqI GGGAC 1 cut(s) 437
FatI CATG 2 cut(s) 228, 404
Fnu4HI GCNGC 1 cut(s) 276
FokI GGATG 3 cut(s) 34, 256, 323
FriOI GRGCYC 1 cut(s) 151
Fsp4HI GCNGC 1 cut(s) 276
GlaI GCGC 1 cut(s) 33
GluI GCNGC 1 cut(s) 276
HaeIII GGCC 1 cut(s) 149
HapII CCGG 1 cut(s) 35
HgaI GACGC 1 cut(s) 38
HhaI GCGC 1 cut(s) 34
Hin1II CATG 2 cut(s) 232, 408
Hin6I GCGC 1 cut(s) 32
HinP1I GCGC 1 cut(s) 32
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 5 cut(s) 23, 221, 302, 323, 385
HpaII CCGG 1 cut(s) 35
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 9 cut(s) 7, 28, 52, 93, 202, 301, 307, 384, 390
Hpy188III TCNNGA 4 cut(s) 119, 191, 281, 430
Hpy8I GTNNAC 1 cut(s) 45
Hpy99I CGWCG 2 cut(s) 32, 138
HpyAV CCTTC 2 cut(s) 116, 310
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 133
HpyCH4V TGCA 3 cut(s) 239, 275, 448
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 113
HpyF3I CTNAG 6 cut(s) 80, 298, 306, 336, 361, 409
HpySE526I ACGT 1 cut(s) 133
Hsp92II CATG 2 cut(s) 232, 408
HspAI GCGC 1 cut(s) 32
Kzo9I GATC 1 cut(s) 458
LmnI GCTCC 1 cut(s) 400
LpnPI CCDG 9 cut(s) 41, 48, 53, 164, 225, 348, 434, 469, 498
Lsp1109I GCAGC 1 cut(s) 287
LweI GCATC 1 cut(s) 234
MaeII ACGT 1 cut(s) 133
MalI GATC 1 cut(s) 460
MboI GATC 1 cut(s) 458
MboII GAAGA 6 cut(s) 26, 216, 352, 355, 445, 501
MhlI GDGCHC 2 cut(s) 151, 236
MluCI AATT 1 cut(s) 425
MlyI GAGTC 3 cut(s) 17, 296, 317
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 7 cut(s) 31, 102, 261, 301, 370, 408, 411
MroXI GAANNNNTTC 1 cut(s) 186
MseI TTAA 1 cut(s) 521
MspI CCGG 1 cut(s) 35
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 2 cut(s) 85, 113
NcoI CCATGG 1 cut(s) 228
NdeII GATC 1 cut(s) 458
NlaIII CATG 2 cut(s) 232, 408
NlaIV GGNNCC 2 cut(s) 149, 453
PdmI GAANNNNTTC 1 cut(s) 186
PfeI GAWTC 2 cut(s) 221, 385
PkrI GCNGC 1 cut(s) 277
PleI GAGTC 3 cut(s) 17, 296, 317
PpsI GAGTC 3 cut(s) 17, 296, 317
PpuMI RGGWCCY 1 cut(s) 451
Psp5II RGGWCCY 1 cut(s) 451
PspN4I GGNNCC 2 cut(s) 149, 453
PspOMI GGGCCC 1 cut(s) 147
PspPI GGNCC 3 cut(s) 147, 148, 451
PspPPI RGGWCCY 1 cut(s) 451
PstI CTGCAG 1 cut(s) 241
PstNI CAGNNNCTG 1 cut(s) 305
SaqAI TTAA 1 cut(s) 521
SatI GCNGC 1 cut(s) 276
Sau3AI GATC 1 cut(s) 458
Sau96I GGNCC 3 cut(s) 147, 148, 451
SchI GAGTC 3 cut(s) 17, 296, 317
SduI GDGCHC 2 cut(s) 151, 236
SetI ASST 7 cut(s) 81, 90, 136, 280, 362, 397, 453
SfaNI GCATC 1 cut(s) 234
SfcI CTRYAG 1 cut(s) 237
SinI GGWCC 1 cut(s) 451
Sse9I AATT 1 cut(s) 425
StyI CCWWGG 2 cut(s) 228, 420
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 1 cut(s) 136
TaqI TCGA 2 cut(s) 326, 482
TasI AATT 1 cut(s) 425
TfiI GAWTC 2 cut(s) 221, 385
Tru1I TTAA 1 cut(s) 521
Tru9I TTAA 1 cut(s) 521
TscAI CASTG 2 cut(s) 241, 474
TseI GCWGC 1 cut(s) 275
TspDTI ATGAA 1 cut(s) 222
TspGWI ACGGA 1 cut(s) 181
TspRI CASTG 2 cut(s) 241, 474
VpaK11BI GGWCC 1 cut(s) 451
XapI RAATTY 1 cut(s) 425
XmnI GAANNNNTTC 1 cut(s) 186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.