FvH4_1g16560

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0004633
Physical Location & Seq
Forward (+)
9547305 .. 9549053
1749 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16560.t1

Sequence Viewer

Length: 1578 bp
ATGGTTGCTGTGTTCAACAAGGAGCTCCTGAGCTGGTACCTGATCACAGTCAAGCTCCGAGAAACAGTTGAATCCGGAATAGCAAATCCAAACCAATCACCAGATCAGAATCTGCAGAAACAAGAGCAGCAACAGTCTGAGTCACTCCAAGTTGTGATCCAAAATGGTGCTGATGGGAAATTTGAAGAAGGGCCGAAATCGCCAGAGTCCGAGTGGGTTCTCAACATCAAAGATAAACTAGAGCAAGCTTCGCAAGACGATGCAGCAAATGTGTGGTCAAAGCTTTGTATCTACAGAATCCCGCATTACCTTAGAGAAGGTGATGACAAAGCTTATGTTCCTCAGATTGTGTCATTGGGGCCTTACCACCATGGAAGGAGACGCCTTCGCCAAATGGACCGCCACAAGTGGCGTTCGCTACATCAAATGCTCAAGAGGACTAATCAGGACATAAAGCTTTACCTTGATTCTATGAAAGAAGTTGAAGAAAAGGCTCGGTCTTGCTATGAAGGGCCGGTTGCAATGAGCCTTAGTAGCAATGAGTTTGTTGAAATGATGGTGCTTGACGGTTGCTTTGTGCTTGAGCTATTCAGGGGCGCAGCTGAGGGGTTTAAGCAACTTGGTTATCCTCGAAATGACCCCATTTTTGCAATGCGTGGCTCGATGCATTCGATTCAGAGAGATATGATCATGCTGGAGAATCAGCTTCCACTTTTCGTTCTTGACCGGCTATTGGGTCTGCAGTTGGGGGACCCTGAACTTAAAGGACTAGTATCCAAGCTAGCATTGAGGTTTTTCGATCCATTGATGCCAACAGATGAGCCATTAACAAAGAGTGATAGGAACAAGTTGGAATCATCACTTGGATATGCAACAACCTTTGATCCATTGTCTGATCAAGGTGCCCTCCATTGTCTTGATGTTTTCCGGCGTAGTTTATTGCGATCGGGACCTAAACCTGTACCAAGAGTTTGGATCAAGAGATGGTCAAATGCTAATCGGGTTGCAGATAAAAGGAGGCAGCAGTTGATTCATTGTGTTACAGAGCTTCGGGAGGCCGGGGTCAAGTTCAAGAAGAGGAAGACTGATCGGTTCTGGGATGTGAAATTCGAAGATGGGATTCTGAGTATACCAAGGCTCTTGGTTCACGATGGTACTAAATCTCTATTCCTGAATCTTATTGCATTTGAGCAGTGTCATCTTGATTGCAGCAATGACATTACCTCCTACGTCATTTTCATGGACAACTTGATCAACTCTCCTGCTGATGTCGGGTATCTACATTATTGTGGCATAATTGAGCACTGGCTTGGCAATGATGCTGAGGTTGCTGATCTGTTTAATCGTCTCTGCCAAGAGGTCGTATTCGACATCAATGACAGTTATCTTTCTCGGCTTTCTGCTGATGTAAACCGCTACTACGACCACCGCTGGAATGCTTGGCGTGCAAGTTTAAAGCATAACTACTTCAGCAACCCTTGGGCCATCCTCTCCTTCCTTGCTGCTGTTGTGTTGCTGCTTCTGACTTTCACTCAAACCTTCTATGGAGTCTACAGCTACTATAGGCCACCGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

526

Amino Acids

60.8

Weight (kDa)

6.93

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 97 - 508 4.7e-136 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 36
AccB1I GGYRCC 2 cut(s) 36, 902
AccI GTMKAC 2 cut(s) 1129, 1551
AccIII TCCGGA 1 cut(s) 74
AciI CCGC 4 cut(s) 302, 400, 1414, 1429
AclWI GGATC 4 cut(s) 151, 794, 878, 983
AcsI RAATTY 2 cut(s) 179, 1106
AcuI CTGAAG 1 cut(s) 1453
AcyI GRCGYC 1 cut(s) 382
AfaI GTAC 3 cut(s) 38, 963, 1156
AfiI CCNNNNNNNGG 1 cut(s) 733
AgsI TTSAA 6 cut(s) 16, 71, 185, 485, 551, 1072
AhlI ACTAGT 1 cut(s) 769
AjuI GAANNNNNNNTTGG 2 cut(s) 846, 878
Alw21I GWGCWC 2 cut(s) 27, 1305
Alw26I GTCTC 2 cut(s) 373, 1352
AlwI GGATC 4 cut(s) 151, 794, 878, 983
AlwNI CAGNNNCTG 1 cut(s) 112
Aor13HI TCCGGA 1 cut(s) 74
AoxI GGCC 6 cut(s) 191, 359, 512, 1056, 1482, 1565
ApeKI GCWGC 7 cut(s) 127, 263, 599, 1021, 1209, 1502, 1516
ApoI RAATTY 2 cut(s) 179, 1106
ArsI GACNNNNNNTTYG 6 cut(s) 260, 292, 557, 589, 629, 661
Asp718I GGTACC 1 cut(s) 36
AspLEI GCGC 1 cut(s) 599
AspS9I GGNCC 7 cut(s) 191, 359, 397, 512, 751, 950, 1482
AsuC2I CCSGG 1 cut(s) 1060
AsuHPI GGTGA 2 cut(s) 90, 332
AsuII TTCGAA 1 cut(s) 1110
AsuNHI GCTAGC 1 cut(s) 781
AvaII GGWCC 3 cut(s) 397, 751, 950
BaeGI GKGCMC 1 cut(s) 907
BanI GGYRCC 2 cut(s) 36, 902
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 1088
Bbv12I GWGCWC 2 cut(s) 27, 1305
BbvCI CCTCAGC 2 cut(s) 603, 1323
BbvI GCAGC 7 cut(s) 139, 275, 611, 1033, 1221, 1489, 1503
BccI CCATC 6 cut(s) 167, 550, 978, 1109, 1145, 1493
BciVI GTATCC 1 cut(s) 784
BclI TGATCA 4 cut(s) 42, 687, 895, 1251
BcnI CCSGG 1 cut(s) 1060
BcoDI GTCTC 2 cut(s) 373, 1352
BcuI ACTAGT 1 cut(s) 769
BfaI CTAG 3 cut(s) 239, 770, 782
BfmI CTRYAG 5 cut(s) 113, 292, 740, 1552, 1561
BfuI GTATCC 1 cut(s) 784
BisI GCNGC 7 cut(s) 128, 264, 600, 1022, 1210, 1503, 1517
BlsI GCNGC 7 cut(s) 129, 265, 601, 1023, 1211, 1504, 1518
Bme1390I CCNGG 1 cut(s) 1060
Bme18I GGWCC 3 cut(s) 397, 751, 950
BmgT120I GGNCC 7 cut(s) 191, 359, 397, 512, 751, 950, 1482
BmiI GGNNCC 6 cut(s) 38, 360, 752, 753, 904, 951
BmrFI CCNGG 1 cut(s) 1060
BmsI GCATC 4 cut(s) 250, 654, 798, 1309
BmtI GCTAGC 1 cut(s) 785
BpiI GAAGAC 1 cut(s) 1088
BpmI CTGGAG 1 cut(s) 716
Bpu10I CCTNAGC 3 cut(s) 29, 603, 1323
Bpu14I TTCGAA 1 cut(s) 1110
BpuEI CTTGAG 2 cut(s) 416, 602
BpuMI CCSGG 1 cut(s) 1060
BsaBI GATNNNNATC 1 cut(s) 108
BsaHI GRCGYC 1 cut(s) 382
BsaJI CCNNGG 4 cut(s) 370, 1059, 1133, 1478
BsaWI WCCGGW 1 cut(s) 74
BsaXI ACNNNNNCTCC 2 cut(s) 1208, 1238
Bsc4I CCNNNNNNNGG 1 cut(s) 733
Bse118I RCCGGY 2 cut(s) 514, 726
Bse1I ACTGG 1 cut(s) 1310
Bse3DI GCAATG 5 cut(s) 528, 544, 657, 1219, 1321
Bse8I GATNNNNATC 1 cut(s) 108
BseAI TCCGGA 1 cut(s) 74
BseDI CCNNGG 4 cut(s) 370, 1059, 1133, 1478
BseGI GGATG 2 cut(s) 1105, 1485
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 733
BseMI GCAATG 5 cut(s) 528, 544, 657, 1219, 1321
BseMII CTCAG 6 cut(s) 20, 129, 356, 594, 1115, 1314
BseNI ACTGG 1 cut(s) 1310
BseSI GKGCMC 1 cut(s) 907
BseXI GCAGC 7 cut(s) 139, 275, 611, 1033, 1221, 1489, 1503
Bsh1285I CGRYCG 1 cut(s) 947
BshFI GGCC 6 cut(s) 193, 361, 514, 1058, 1484, 1567
BshNI GGYRCC 2 cut(s) 36, 902
BsiEI CGRYCG 1 cut(s) 947
BsiHKAI GWGCWC 2 cut(s) 27, 1305
BsiSI CCGG 5 cut(s) 75, 515, 727, 928, 1059
BslFI GGGAC 2 cut(s) 764, 963
BslI CCNNNNNNNGG 1 cut(s) 733
BsmAI GTCTC 2 cut(s) 373, 1352
BsmBI CGTCTC 2 cut(s) 373, 1352
BsmFI GGGAC 2 cut(s) 764, 963
BsmI GAATGC 2 cut(s) 667, 1441
BsnI GGCC 6 cut(s) 193, 361, 514, 1058, 1484, 1567
Bsp119I TTCGAA 1 cut(s) 1110
Bsp1286I GDGCHC 3 cut(s) 27, 907, 1305
Bsp13I TCCGGA 1 cut(s) 74
Bsp19I CCATGG 1 cut(s) 370
BspACI CCGC 4 cut(s) 302, 400, 1414, 1429
BspANI GGCC 6 cut(s) 193, 361, 514, 1058, 1484, 1567
BspCNI CTCAG 6 cut(s) 21, 130, 355, 595, 1116, 1315
BspEI TCCGGA 1 cut(s) 74
BspLI GGNNCC 6 cut(s) 38, 360, 752, 753, 904, 951
BspMAI CTGCAG 2 cut(s) 117, 744
BspOI GCTAGC 1 cut(s) 785
BspPI GGATC 4 cut(s) 151, 794, 878, 983
BspT104I TTCGAA 1 cut(s) 1110
BspT107I GGYRCC 2 cut(s) 36, 902
BsrDI GCAATG 5 cut(s) 528, 544, 657, 1219, 1321
BsrFI RCCGGY 2 cut(s) 514, 726
BsrI ACTGG 1 cut(s) 1310
BssAI RCCGGY 2 cut(s) 514, 726
BssECI CCNNGG 4 cut(s) 370, 1059, 1133, 1478
BssNAI GTATAC 1 cut(s) 1130
BssNI GRCGYC 1 cut(s) 382
BssT1I CCWWGG 3 cut(s) 370, 1133, 1478
Bst1107I GTATAC 1 cut(s) 1130
Bst4CI ACNGT 5 cut(s) 49, 67, 135, 569, 1382
Bst6I CTCTTC 1 cut(s) 1070
BstACI GRCGYC 1 cut(s) 382
BstBI TTCGAA 1 cut(s) 1110
BstC8I GCNNGC 3 cut(s) 246, 783, 1446
BstDEI CTNAG 8 cut(s) 29, 138, 311, 342, 530, 603, 1124, 1323
BstDSI CCRYGG 1 cut(s) 370
BstF5I GGATG 2 cut(s) 1105, 1485
BstHHI GCGC 1 cut(s) 599
BstMAI GTCTC 2 cut(s) 373, 1352
BstMCI CGRYCG 1 cut(s) 947
BstMWI GCNNNNNNNGC 5 cut(s) 199, 250, 534, 1328, 1445
BstSCI CCNGG 1 cut(s) 1058
BstSFI CTRYAG 5 cut(s) 113, 292, 740, 1552, 1561
BstSLI GKGCMC 1 cut(s) 907
BstV1I GCAGC 7 cut(s) 139, 275, 611, 1033, 1221, 1489, 1503
BstV2I GAAGAC 1 cut(s) 1088
BstXI CCANNNNNNTGG 1 cut(s) 972
BstZ17I GTATAC 1 cut(s) 1130
BsuI GTATCC 1 cut(s) 784
BsuRI GGCC 6 cut(s) 193, 361, 514, 1058, 1484, 1567
BtgI CCRYGG 1 cut(s) 370
BtsCI GGATG 2 cut(s) 1105, 1485
BtsI GCAGTG 1 cut(s) 1199
BtsIMutI CAGTG 2 cut(s) 1199, 1303
Cac8I GCNNGC 3 cut(s) 246, 783, 1446
CaiI CAGNNNCTG 1 cut(s) 112
CfoI GCGC 1 cut(s) 599
Cfr10I RCCGGY 2 cut(s) 514, 726
Cfr13I GGNCC 7 cut(s) 191, 359, 397, 512, 751, 950, 1482
CseI GACGC 1 cut(s) 390
Csp6I GTAC 3 cut(s) 37, 962, 1155
CviAII CATG 3 cut(s) 371, 691, 1240
CviQI GTAC 3 cut(s) 37, 962, 1155
DdeI CTNAG 8 cut(s) 29, 138, 311, 342, 530, 603, 1124, 1323
DraI TTTAAA 1 cut(s) 1455
Eam1104I CTCTTC 1 cut(s) 1070
EarI CTCTTC 1 cut(s) 1070
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 3 cut(s) 370, 1133, 1478
Eco24I GRGCYC 1 cut(s) 27
Eco47I GGWCC 3 cut(s) 397, 751, 950
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 1453
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 3 cut(s) 359, 751, 950
EcoT14I CCWWGG 3 cut(s) 370, 1133, 1478
EcoT22I ATGCAT 1 cut(s) 669
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 3 cut(s) 370, 1133, 1478
Esp3I CGTCTC 2 cut(s) 373, 1352
FaeI CATG 3 cut(s) 374, 694, 1243
FaqI GGGAC 2 cut(s) 764, 963
FatI CATG 3 cut(s) 370, 690, 1239
FauI CCCGC 1 cut(s) 309
FbaI TGATCA 4 cut(s) 42, 687, 895, 1251
FblI GTMKAC 2 cut(s) 1129, 1551
Fnu4HI GCNGC 7 cut(s) 128, 264, 600, 1022, 1210, 1503, 1517
FokI GGATG 2 cut(s) 1112, 1472
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 7 cut(s) 128, 264, 600, 1022, 1210, 1503, 1517
FspBI CTAG 3 cut(s) 239, 770, 782
GlaI GCGC 1 cut(s) 598
GluI GCNGC 7 cut(s) 128, 264, 600, 1022, 1210, 1503, 1517
GsuI CTGGAG 1 cut(s) 716
HaeIII GGCC 6 cut(s) 193, 361, 514, 1058, 1484, 1567
HapII CCGG 5 cut(s) 75, 515, 727, 928, 1059
HgaI GACGC 1 cut(s) 390
HhaI GCGC 1 cut(s) 599
Hin1I GRCGYC 1 cut(s) 382
Hin1II CATG 3 cut(s) 374, 694, 1243
Hin6I GCGC 1 cut(s) 597
HinP1I GCGC 1 cut(s) 597
HindIII AAGCTT 4 cut(s) 246, 281, 330, 455
HpaII CCGG 5 cut(s) 75, 515, 727, 928, 1059
HphI GGTGA 2 cut(s) 90, 332
Hpy166II GTNNAC 4 cut(s) 1130, 1147, 1411, 1552
Hpy188I TCNGA 9 cut(s) 59, 108, 139, 211, 345, 678, 895, 1125, 1524
Hpy8I GTNNAC 4 cut(s) 1130, 1147, 1411, 1552
HpyAV CCTTC 7 cut(s) 182, 311, 369, 395, 503, 1504, 1549
HpyCH4III ACNGT 5 cut(s) 49, 67, 135, 569, 1382
HpyCH4IV ACGT 1 cut(s) 1230
HpyF10VI GCNNNNNNNGC 5 cut(s) 199, 250, 534, 1328, 1445
HpyF3I CTNAG 8 cut(s) 29, 138, 311, 342, 530, 603, 1124, 1323
HpySE526I ACGT 1 cut(s) 1230
Hsp92I GRCGYC 1 cut(s) 382
Hsp92II CATG 3 cut(s) 374, 694, 1243
HspAI GCGC 1 cut(s) 597
KflI GGGWCCC 1 cut(s) 751
Kpn2I TCCGGA 1 cut(s) 74
KpnI GGTACC 1 cut(s) 40
Ksp22I TGATCA 4 cut(s) 42, 687, 895, 1251
LmnI GCTCC 3 cut(s) 22, 30, 60
Lsp1109I GCAGC 7 cut(s) 139, 275, 611, 1033, 1221, 1489, 1503
LweI GCATC 4 cut(s) 250, 654, 798, 1309
MaeI CTAG 3 cut(s) 239, 770, 782
MaeII ACGT 1 cut(s) 1230
MaeIII GTNAC 2 cut(s) 141, 1039
MboII GAAGA 5 cut(s) 197, 497, 1087, 1093, 1124
MhlI GDGCHC 3 cut(s) 27, 907, 1305
MluCI AATT 4 cut(s) 179, 1106, 1296, 1573
MlyI GAGTC 3 cut(s) 149, 215, 1557
MmeI TCCRAC 1 cut(s) 831
Mph1103I ATGCAT 1 cut(s) 669
MroI TCCGGA 1 cut(s) 74
MseI TTAA 6 cut(s) 612, 762, 827, 1341, 1454, 1576
MslI CAYNNNNRTG 2 cut(s) 1238, 1287
MspA1I CMGCKG 2 cut(s) 602, 1431
MspI CCGG 5 cut(s) 75, 515, 727, 928, 1059
MspR9I CCNGG 1 cut(s) 1060
Mva1269I GAATGC 2 cut(s) 667, 1441
MwoI GCNNNNNNNGC 5 cut(s) 199, 250, 534, 1328, 1445
NciI CCSGG 1 cut(s) 1060
NcoI CCATGG 1 cut(s) 370
NheI GCTAGC 1 cut(s) 781
NlaIII CATG 3 cut(s) 374, 694, 1243
NlaIV GGNNCC 6 cut(s) 38, 360, 752, 753, 904, 951
NmeAIII GCCGAG 1 cut(s) 1372
NmuCI GTSAC 1 cut(s) 141
NsiI ATGCAT 1 cut(s) 669
NspV TTCGAA 1 cut(s) 1110
PcsI WCGNNNNNNNCGW 1 cut(s) 668
PctI GAATGC 2 cut(s) 667, 1441
PkrI GCNGC 7 cut(s) 129, 265, 601, 1023, 1211, 1504, 1518
Ple19I CGATCG 1 cut(s) 947
PleI GAGTC 3 cut(s) 148, 214, 1556
PpsI GAGTC 3 cut(s) 148, 214, 1556
PpuMI RGGWCCY 2 cut(s) 751, 950
Psp124BI GAGCTC 1 cut(s) 27
Psp5II RGGWCCY 2 cut(s) 751, 950
PspN4I GGNNCC 6 cut(s) 38, 360, 752, 753, 904, 951
PspPI GGNCC 7 cut(s) 191, 359, 397, 512, 751, 950, 1482
PspPPI RGGWCCY 2 cut(s) 751, 950
PstI CTGCAG 2 cut(s) 117, 744
PstNI CAGNNNCTG 1 cut(s) 112
PvuI CGATCG 1 cut(s) 947
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 3 cut(s) 38, 963, 1156
RsaNI GTAC 3 cut(s) 37, 962, 1155
RseI CAYNNNNRTG 2 cut(s) 1238, 1287
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 6 cut(s) 612, 762, 827, 1341, 1454, 1576
SatI GCNGC 7 cut(s) 128, 264, 600, 1022, 1210, 1503, 1517
Sau96I GGNCC 7 cut(s) 191, 359, 397, 512, 751, 950, 1482
SchI GAGTC 3 cut(s) 149, 215, 1557
ScrFI CCNGG 1 cut(s) 1060
SduI GDGCHC 3 cut(s) 27, 907, 1305
SfaNI GCATC 4 cut(s) 250, 654, 798, 1309
SfcI CTRYAG 5 cut(s) 113, 292, 740, 1552, 1561
SfuI TTCGAA 1 cut(s) 1110
SinI GGWCC 3 cut(s) 397, 751, 950
SmiMI CAYNNNNRTG 2 cut(s) 1238, 1287
SmlI CTYRAG 2 cut(s) 431, 581
SmoI CTYRAG 2 cut(s) 431, 581
SpeI ACTAGT 1 cut(s) 769
Sse9I AATT 4 cut(s) 179, 1106, 1296, 1573
SsiI CCGC 4 cut(s) 302, 400, 1414, 1429
SspMI CTAG 3 cut(s) 239, 770, 782
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 1 cut(s) 1058
StyI CCWWGG 3 cut(s) 370, 1133, 1478
TaaI ACNGT 5 cut(s) 49, 67, 135, 569, 1382
TaiI ACGT 1 cut(s) 1233
TaqI TCGA 6 cut(s) 631, 662, 671, 798, 1110, 1368
TaqII GACCGA 1 cut(s) 486
TasI AATT 4 cut(s) 179, 1106, 1296, 1573
Tru1I TTAA 6 cut(s) 612, 762, 827, 1341, 1454, 1576
Tru9I TTAA 6 cut(s) 612, 762, 827, 1341, 1454, 1576
TscAI CASTG 2 cut(s) 1199, 1310
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 7 cut(s) 127, 263, 599, 1021, 1209, 1502, 1516
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 4 cut(s) 488, 522, 1022, 1228
TspRI CASTG 2 cut(s) 1199, 1310
VpaK11BI GGWCC 3 cut(s) 397, 751, 950
XapI RAATTY 2 cut(s) 179, 1106
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
XmiI GTMKAC 2 cut(s) 1129, 1551
XspI CTAG 3 cut(s) 239, 770, 782
Zsp2I ATGCAT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.