MD15G1287600.v1.1

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0004633
Physical Location & Seq
Forward (+)
26440840 .. 26441316
477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1287600.v1.1.491

Sequence Viewer

Length: 477 bp
ATGACAAAGCTAATGTGCCTGAAATTGTGTCACTACCATCACGGCAAGAGGCGCCTTCGCCAAAGGGACCGCCACAAATGGCGGTCGCTTCATCTAATGCTTAAGCGGACAAACCAGTTCATAGAGCTGTATCTTGATTCTATAAAAGAAGTTGAAGAAAAGGCTCGAGCTTGCTACGAAGGGCCAGTTGCAATGGGGCTCAGCAGCAACGAGTTCGTAGAAAAGATGGTGCTTGACGGTTGCTTTGTGCTCGAGCTGTTTCGAGGTGCTGCCGAGGGATTTAAGCAACTAGGTTACCCTCGAAATGATCCCATATTTGCAATGCGTGGCACAATGCATTCGATTCAGAGGGACATGATCATGCTTGAGAATCAGCTTCCGTTTTTCATTCTTTATTGGTTATTGGTGCTGCAGTATGGAGATCCTGAGCAGAAAGGACTTGTAGCTAAGCTAGACTCGGGGTTTTCGATCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

159

Amino Acids

18.61

Weight (kDa)

9.08

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 12 - 143 1.3e-37 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 51
AciI CCGC 3 cut(s) 70, 82, 106
AclWI GGATC 3 cut(s) 302, 416, 463
AcyI GRCGYC 1 cut(s) 52
AflII CTTAAG 1 cut(s) 101
AgsI TTSAA 1 cut(s) 155
AluBI AGCT 7 cut(s) 10, 127, 170, 256, 376, 446, 451
AluI AGCT 7 cut(s) 10, 127, 170, 256, 376, 446, 451
Alw21I GWGCWC 1 cut(s) 252
AlwI GGATC 3 cut(s) 302, 416, 463
Ama87I CYCGRG 3 cut(s) 165, 251, 457
AoxI GGCC 1 cut(s) 182
ApeKI GCWGC 3 cut(s) 204, 269, 409
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 1 cut(s) 54
AspS9I GGNCC 2 cut(s) 67, 182
AvaI CYCGRG 3 cut(s) 165, 251, 457
AvaII GGWCC 1 cut(s) 67
BanI GGYRCC 1 cut(s) 51
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 252
BbvI GCAGC 3 cut(s) 216, 256, 396
BccI CCATC 2 cut(s) 45, 220
BceAI ACGGC 1 cut(s) 58
BcgI CGANNNNNNTGC 2 cut(s) 196, 230
BclI TGATCA 1 cut(s) 357
BfaI CTAG 2 cut(s) 290, 452
BfmI CTRYAG 1 cut(s) 410
BfoI RGCGCY 1 cut(s) 55
BfrI CTTAAG 1 cut(s) 101
BisI GCNGC 3 cut(s) 205, 270, 410
BlpI GCTNAGC 2 cut(s) 200, 447
BlsI GCNGC 3 cut(s) 206, 271, 411
Bme18I GGWCC 1 cut(s) 67
BmeT110I CYCGRG 3 cut(s) 165, 251, 457
BmgT120I GGNCC 2 cut(s) 67, 182
BmiI GGNNCC 2 cut(s) 53, 68
Bpu10I CCTNAGC 1 cut(s) 426
Bpu1102I GCTNAGC 2 cut(s) 200, 447
BpuEI CTTGAG 1 cut(s) 386
BsaHI GRCGYC 1 cut(s) 52
BsaJI CCNNGG 1 cut(s) 273
Bse1I ACTGG 2 cut(s) 115, 185
Bse3DI GCAATG 2 cut(s) 198, 327
BseDI CCNNGG 1 cut(s) 273
BseMI GCAATG 2 cut(s) 198, 327
BseMII CTCAG 2 cut(s) 214, 417
BseNI ACTGG 2 cut(s) 115, 185
BseXI GCAGC 3 cut(s) 216, 256, 396
Bsh1285I CGRYCG 1 cut(s) 86
BshFI GGCC 1 cut(s) 184
BshNI GGYRCC 1 cut(s) 51
BsiEI CGRYCG 1 cut(s) 86
BsiHKAI GWGCWC 1 cut(s) 252
BsiHKCI CYCGRG 3 cut(s) 165, 251, 457
BslFI GGGAC 2 cut(s) 80, 365
BsmFI GGGAC 2 cut(s) 80, 365
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 1 cut(s) 184
BsoBI CYCGRG 3 cut(s) 165, 251, 457
Bsp1286I GDGCHC 2 cut(s) 201, 252
Bsp143I GATC 4 cut(s) 307, 357, 421, 468
Bsp1720I GCTNAGC 2 cut(s) 200, 447
BspACI CCGC 3 cut(s) 70, 82, 106
BspANI GGCC 1 cut(s) 184
BspCNI CTCAG 2 cut(s) 213, 418
BspLI GGNNCC 2 cut(s) 53, 68
BspMAI CTGCAG 1 cut(s) 414
BspPI GGATC 3 cut(s) 302, 416, 463
BspT107I GGYRCC 1 cut(s) 51
BspTI CTTAAG 1 cut(s) 101
BsrDI GCAATG 2 cut(s) 198, 327
BsrI ACTGG 2 cut(s) 115, 185
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 4 cut(s) 307, 357, 421, 468
BssNI GRCGYC 1 cut(s) 52
Bst4CI ACNGT 1 cut(s) 239
BstACI GRCGYC 1 cut(s) 52
BstAFI CTTAAG 1 cut(s) 101
BstC8I GCNNGC 1 cut(s) 172
BstDEI CTNAG 3 cut(s) 200, 426, 447
BstEII GGTNACC 1 cut(s) 293
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 1 cut(s) 54
BstKTI GATC 4 cut(s) 310, 360, 424, 471
BstMBI GATC 4 cut(s) 307, 357, 421, 468
BstMCI CGRYCG 1 cut(s) 86
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstPI GGTNACC 1 cut(s) 293
BstSFI CTRYAG 1 cut(s) 410
BstV1I GCAGC 3 cut(s) 216, 256, 396
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuRI GGCC 1 cut(s) 184
Cac8I GCNNGC 1 cut(s) 172
CfoI GCGC 1 cut(s) 54
Cfr13I GGNCC 2 cut(s) 67, 182
CviAII CATG 2 cut(s) 355, 361
DdeI CTNAG 3 cut(s) 200, 426, 447
DinI GGCGCC 1 cut(s) 53
DpnI GATC 4 cut(s) 309, 359, 423, 470
DpnII GATC 4 cut(s) 307, 357, 421, 468
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 67
Eco88I CYCGRG 3 cut(s) 165, 251, 457
Eco91I GGTNACC 1 cut(s) 293
EcoO65I GGTNACC 1 cut(s) 293
EcoT22I ATGCAT 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 201
EgeI GGCGCC 1 cut(s) 53
EheI GGCGCC 1 cut(s) 53
FaeI CATG 2 cut(s) 358, 364
FaiI YATR 6 cut(s) 122, 143, 314, 356, 362, 417
FaqI GGGAC 2 cut(s) 80, 365
FatI CATG 2 cut(s) 354, 360
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 3 cut(s) 205, 270, 410
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 3 cut(s) 205, 270, 410
FspBI CTAG 2 cut(s) 290, 452
GlaI GCGC 1 cut(s) 53
GluI GCNGC 3 cut(s) 205, 270, 410
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 1 cut(s) 184
HhaI GCGC 1 cut(s) 54
Hin1I GRCGYC 1 cut(s) 52
Hin1II CATG 2 cut(s) 358, 364
Hin6I GCGC 1 cut(s) 52
HinP1I GCGC 1 cut(s) 52
HinfI GANTC 4 cut(s) 137, 343, 370, 455
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 2 cut(s) 134, 425
HpyAV CCTTC 2 cut(s) 65, 173
HpyCH4III ACNGT 1 cut(s) 239
HpyCH4V TGCA 4 cut(s) 191, 320, 337, 412
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 3 cut(s) 200, 426, 447
Hsp92I GRCGYC 1 cut(s) 52
Hsp92II CATG 2 cut(s) 358, 364
HspAI GCGC 1 cut(s) 52
KasI GGCGCC 1 cut(s) 51
Ksp22I TGATCA 1 cut(s) 357
Kzo9I GATC 4 cut(s) 307, 357, 421, 468
LpnPI CCDG 4 cut(s) 32, 128, 198, 438
Lsp1109I GCAGC 3 cut(s) 216, 256, 396
MaeI CTAG 2 cut(s) 290, 452
MaeIII GTNAC 2 cut(s) 29, 293
MalI GATC 4 cut(s) 309, 359, 423, 470
MboI GATC 4 cut(s) 307, 357, 421, 468
MboII GAAGA 1 cut(s) 167
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 201, 252
MluCI AATT 1 cut(s) 23
Mly113I GGCGCC 1 cut(s) 52
MlyI GAGTC 1 cut(s) 449
MnlI CCTC 5 cut(s) 42, 257, 268, 309, 342
Mph1103I ATGCAT 1 cut(s) 339
MseI TTAA 2 cut(s) 102, 282
MslI CAYNNNNRTG 1 cut(s) 359
MspCI CTTAAG 1 cut(s) 101
Mva1269I GAATGC 1 cut(s) 337
MwoI GCNNNNNNNGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 52
NdeII GATC 4 cut(s) 307, 357, 421, 468
NlaIII CATG 2 cut(s) 358, 364
NlaIV GGNNCC 2 cut(s) 53, 68
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 29
NsiI ATGCAT 1 cut(s) 339
PaeR7I CTCGAG 2 cut(s) 165, 251
PcsI WCGNNNNNNNCGW 1 cut(s) 464
PctI GAATGC 1 cut(s) 337
PfeI GAWTC 3 cut(s) 137, 343, 370
PkrI GCNGC 3 cut(s) 206, 271, 411
PleI GAGTC 1 cut(s) 449
PluTI GGCGCC 1 cut(s) 55
PpsI GAGTC 1 cut(s) 449
PspEI GGTNACC 1 cut(s) 293
PspN4I GGNNCC 2 cut(s) 53, 68
PspPI GGNCC 2 cut(s) 67, 182
PspXI VCTCGAGB 2 cut(s) 165, 251
PstI CTGCAG 1 cut(s) 414
PsuI RGATCY 1 cut(s) 421
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 2 cut(s) 102, 282
SatI GCNGC 3 cut(s) 205, 270, 410
Sau3AI GATC 4 cut(s) 307, 357, 421, 468
Sau96I GGNCC 2 cut(s) 67, 182
SchI GAGTC 1 cut(s) 449
SduI GDGCHC 2 cut(s) 201, 252
SetI ASST 9 cut(s) 12, 129, 172, 258, 268, 295, 378, 448, 453
SfcI CTRYAG 1 cut(s) 410
SfoI GGCGCC 1 cut(s) 53
Sfr274I CTCGAG 2 cut(s) 165, 251
SinI GGWCC 1 cut(s) 67
SlaI CTCGAG 2 cut(s) 165, 251
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 4 cut(s) 101, 165, 251, 365
SmoI CTYRAG 4 cut(s) 101, 165, 251, 365
Sse9I AATT 1 cut(s) 23
SsiI CCGC 3 cut(s) 70, 82, 106
SspDI GGCGCC 1 cut(s) 51
SspMI CTAG 2 cut(s) 290, 452
TaaI ACNGT 1 cut(s) 239
TaqI TCGA 6 cut(s) 166, 252, 262, 301, 341, 467
TasI AATT 1 cut(s) 23
TfiI GAWTC 3 cut(s) 137, 343, 370
Tru1I TTAA 2 cut(s) 102, 282
Tru9I TTAA 2 cut(s) 102, 282
TseFI GTSAC 1 cut(s) 29
TseI GCWGC 3 cut(s) 204, 269, 409
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 3 cut(s) 80, 109, 376
TspGWI ACGGA 1 cut(s) 369
Vha464I CTTAAG 1 cut(s) 101
VpaK11BI GGWCC 1 cut(s) 67
XhoI CTCGAG 2 cut(s) 165, 251
XspI CTAG 2 cut(s) 290, 452
Zsp2I ATGCAT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.