Rh2AG186900

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0004633
Physical Location & Seq
Forward (+)
18292858 .. 18294444
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG186900.1

Sequence Viewer

Length: 1305 bp
ATGGTTGCTGTGTTCAACAAGGAGCTCCTGAGCTGGTACCTGATCACAGTCAAGCTCCGAGAAACAGTTGAATCCGGAATAGCAAATCCGAACCAATCAATCCAATCTCCTGATCAGAATCTGCAGAAACGAGAGCAGCAACAATCTCAGTCACTCCAAGTTGTGATCCAAAATGGTGGTGATGGGAAATTTGAAGAAGGGCCGAAATCGCCAGAGTCCGAGTGGGTTCTCACCATCAAAGATAAACTCGAGCAAGCTTCTCAAGACGATGCAGCAAATGTGTGGTCAAAGCTCTGCATCTACAGAGTCCCACATTACCTGAGAGAAGCTGATGGGTTTAAGCAACTCGGGTACCCTCGAAACGATCCCATTTTTGCAATGCGTGGCTCGATGCATTCGATTCAGAGGGACATGATTATGCTTGAGAATCAGCTTCCACTTTTCGTTCTTGATCGGCTATTGGGTCTGCAGTTGGGGGATCCTGAGCTTAAAGGACTTGTATCCAAGCTAGCATTGAGGTTTTTCGATCCATTAATGCCAACAGATGAGCCATTAACAAAGAGTGATAGGAACAAATTGGAGTCATCACTGGGATATGCAACAACCTTTGATCCATTGTCTGATCAAGGTGCCCTTCATTGCCTTGATGTTTTCCGACGTAGTCTATTGCGATCAGGGCCGAAACCTGTGCCAAGAGTTTGGATCAAGAGATGGTCAAACGTGAATCGGGTTGCCGATAAAAGGAGGACACAGTTGATTCATTGTGTCACAGAGCTTAGGGAGGCCGGGATCAAGTTCAAGAAGAGGAAGACTGATCGGTTCTGGGATGTGAAATTTGAAGATGGGATTCTGAGTATTCCAAGGCTCTTGATCCACGATGGTACTAAATCTCTATTCCTCAATCTTATTGCATTTGAGCAGTGTCATCTTGATTGCAGCAATGACATTACCTCCTATGTCATCTTCATGGACAACTTGATCAACTCTCCTGCTGATGTGGGTTATCTACATTATTGTGGCATAATTGAACACTGGCTTGGCAGTGATGCTGAGGTTGCTGACCTATTTAATCGTCTCTGCCAAGAGGTGGTCTTCGACATCAATGACAGTTATCTTTCTCGGCTTTCGGCTGATGTAAACCGCTACTACGACCACCGGTGGAATGCATGGCGTGCCAGTTTAAAGCACAACTACTTCAGCAATCCCTGGGCCATCCTCTCCTTCCTTGCTGCTGTTGTGTTGCTGTTTCTGACTTTCACTCAAACCTTCTATGGAGTTTATAGCTACTATAGACCGCCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

434

Amino Acids

50.12

Weight (kDa)

6.8

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 119 - 417 3.6e-91 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 36, 351
AccB1I GGYRCC 3 cut(s) 36, 351, 629
AccB7I CCANNNNNTGG 1 cut(s) 1087
AccIII TCCGGA 1 cut(s) 74
AciI CCGC 2 cut(s) 1141, 1295
AclWI GGATC 9 cut(s) 160, 359, 473, 486, 521, 605, 710, 797, 865
AcsI RAATTY 2 cut(s) 188, 833
AcuI CTGAAG 1 cut(s) 1180
AfaI GTAC 3 cut(s) 38, 353, 883
AfiI CCNNNNNNNGG 2 cut(s) 741, 1087
AgeI ACCGGT 1 cut(s) 1155
AgsI TTSAA 6 cut(s) 16, 71, 194, 799, 839, 1028
AjnI CCWGG 1 cut(s) 1205
AjuI GAANNNNNNNTTGG 2 cut(s) 1020, 1052
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 1 cut(s) 1079
AlwI GGATC 9 cut(s) 160, 359, 473, 486, 521, 605, 710, 797, 865
AlwNI CAGNNNCTG 1 cut(s) 121
Ama87I CYCGRG 2 cut(s) 248, 347
Aor13HI TCCGGA 1 cut(s) 74
AoxI GGCC 4 cut(s) 200, 677, 783, 1209
ApeKI GCWGC 4 cut(s) 136, 272, 936, 1229
ApoI RAATTY 2 cut(s) 188, 833
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AseI ATTAAT 1 cut(s) 533
AsiGI ACCGGT 1 cut(s) 1155
Asp718I GGTACC 2 cut(s) 36, 351
AspS9I GGNCC 3 cut(s) 200, 677, 1209
AsuC2I CCSGG 1 cut(s) 787
AsuHPI GGTGA 2 cut(s) 191, 223
AsuNHI GCTAGC 1 cut(s) 508
AvaI CYCGRG 2 cut(s) 248, 347
BaeGI GKGCMC 1 cut(s) 634
BaeI ACNNNNGTAYC 2 cut(s) 335, 368
BamHI GGATCC 1 cut(s) 478
BanI GGYRCC 3 cut(s) 36, 351, 629
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 2 cut(s) 815, 1084
Bbv12I GWGCWC 1 cut(s) 27
BbvCI CCTCAGC 1 cut(s) 1050
BbvI GCAGC 4 cut(s) 148, 284, 948, 1216
BccI CCATC 7 cut(s) 176, 242, 326, 705, 836, 872, 1220
BcgI CGANNNNNNTGC 2 cut(s) 670, 704
BciT130I CCWGG 1 cut(s) 1207
BciVI GTATCC 1 cut(s) 511
BclI TGATCA 4 cut(s) 42, 112, 622, 978
BcnI CCSGG 1 cut(s) 787
BcoDI GTCTC 1 cut(s) 1079
BfaI CTAG 2 cut(s) 509, 1303
BfmI CTRYAG 4 cut(s) 122, 301, 467, 1288
BfuI GTATCC 1 cut(s) 511
BisI GCNGC 4 cut(s) 137, 273, 937, 1230
BlsI GCNGC 4 cut(s) 138, 274, 938, 1231
Bme1390I CCNGG 2 cut(s) 787, 1207
BmeT110I CYCGRG 2 cut(s) 248, 347
BmgT120I GGNCC 3 cut(s) 200, 677, 1209
BmiI GGNNCC 4 cut(s) 38, 353, 480, 631
BmrFI CCNGG 2 cut(s) 787, 1207
BmrI ACTGGG 1 cut(s) 599
BmsI GCATC 4 cut(s) 259, 306, 381, 1036
BmtI GCTAGC 1 cut(s) 512
BmuI ACTGGG 1 cut(s) 599
BpiI GAAGAC 2 cut(s) 815, 1084
Bpu10I CCTNAGC 4 cut(s) 29, 483, 776, 1050
BpuEI CTTGAG 2 cut(s) 246, 443
BpuMI CCSGG 1 cut(s) 787
BsaBI GATNNNNATC 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 860, 1205, 1206
BsaWI WCCGGW 2 cut(s) 74, 1155
BsaXI ACNNNNNCTCC 2 cut(s) 935, 965
Bsc4I CCNNNNNNNGG 2 cut(s) 741, 1087
Bse118I RCCGGY 1 cut(s) 1155
Bse1I ACTGG 3 cut(s) 594, 1037, 1176
Bse3DI GCAATG 3 cut(s) 384, 637, 946
Bse8I GATNNNNATC 1 cut(s) 117
BseAI TCCGGA 1 cut(s) 74
BseBI CCWGG 1 cut(s) 1207
BseDI CCNNGG 3 cut(s) 860, 1205, 1206
BseGI GGATG 2 cut(s) 832, 1212
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 2 cut(s) 741, 1087
BseMI GCAATG 3 cut(s) 384, 637, 946
BseMII CTCAG 6 cut(s) 20, 161, 311, 474, 842, 1041
BseNI ACTGG 3 cut(s) 594, 1037, 1176
BseSI GKGCMC 1 cut(s) 634
BseXI GCAGC 4 cut(s) 148, 284, 948, 1216
BshFI GGCC 4 cut(s) 202, 679, 785, 1211
BshNI GGYRCC 3 cut(s) 36, 351, 629
BshTI ACCGGT 1 cut(s) 1155
BsiHKAI GWGCWC 1 cut(s) 27
BsiHKCI CYCGRG 2 cut(s) 248, 347
BsiSI CCGG 3 cut(s) 75, 786, 1156
BslFI GGGAC 2 cut(s) 293, 422
BslI CCNNNNNNNGG 2 cut(s) 741, 1087
BsmAI GTCTC 1 cut(s) 1079
BsmBI CGTCTC 1 cut(s) 1079
BsmFI GGGAC 2 cut(s) 293, 422
BsmI GAATGC 2 cut(s) 394, 1168
BsnI GGCC 4 cut(s) 202, 679, 785, 1211
BsoBI CYCGRG 2 cut(s) 248, 347
Bsp1286I GDGCHC 2 cut(s) 27, 634
Bsp13I TCCGGA 1 cut(s) 74
BspACI CCGC 2 cut(s) 1141, 1295
BspANI GGCC 4 cut(s) 202, 679, 785, 1211
BspCNI CTCAG 6 cut(s) 21, 160, 312, 475, 843, 1042
BspEI TCCGGA 1 cut(s) 74
BspLI GGNNCC 4 cut(s) 38, 353, 480, 631
BspMAI CTGCAG 2 cut(s) 126, 471
BspOI GCTAGC 1 cut(s) 512
BspPI GGATC 9 cut(s) 160, 359, 473, 486, 521, 605, 710, 797, 865
BspT107I GGYRCC 3 cut(s) 36, 351, 629
BsrDI GCAATG 3 cut(s) 384, 637, 946
BsrFI RCCGGY 1 cut(s) 1155
BsrI ACTGG 3 cut(s) 594, 1037, 1176
BssAI RCCGGY 1 cut(s) 1155
BssECI CCNNGG 3 cut(s) 860, 1205, 1206
BssT1I CCWWGG 1 cut(s) 860
Bst2UI CCWGG 1 cut(s) 1207
Bst4CI ACNGT 4 cut(s) 49, 67, 753, 1109
Bst6I CTCTTC 1 cut(s) 797
BstAPI GCANNNNNTGC 1 cut(s) 1172
BstC8I GCNNGC 3 cut(s) 255, 510, 1173
BstDEI CTNAG 7 cut(s) 29, 147, 320, 483, 776, 851, 1050
BstF5I GGATG 2 cut(s) 832, 1212
BstMAI GTCTC 1 cut(s) 1079
BstMWI GCNNNNNNNGC 4 cut(s) 208, 676, 1055, 1172
BstNI CCWGG 1 cut(s) 1207
BstSCI CCNGG 2 cut(s) 785, 1205
BstSFI CTRYAG 4 cut(s) 122, 301, 467, 1288
BstSLI GKGCMC 1 cut(s) 634
BstV1I GCAGC 4 cut(s) 148, 284, 948, 1216
BstV2I GAAGAC 2 cut(s) 815, 1084
BstX2I RGATCY 1 cut(s) 478
BstXI CCANNNNNNTGG 2 cut(s) 176, 699
BstYI RGATCY 1 cut(s) 478
BsuI GTATCC 1 cut(s) 511
BsuRI GGCC 4 cut(s) 202, 679, 785, 1211
BtsCI GGATG 2 cut(s) 832, 1212
BtsI GCAGTG 2 cut(s) 926, 1048
BtsIMutI CAGTG 4 cut(s) 587, 926, 1030, 1048
Cac8I GCNNGC 3 cut(s) 255, 510, 1173
CaiI CAGNNNCTG 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 1155
Cfr13I GGNCC 3 cut(s) 200, 677, 1209
Csp6I GTAC 3 cut(s) 37, 352, 882
CspAI ACCGGT 1 cut(s) 1155
CviAII CATG 3 cut(s) 412, 967, 1167
CviQI GTAC 3 cut(s) 37, 352, 882
DdeI CTNAG 7 cut(s) 29, 147, 320, 483, 776, 851, 1050
DraI TTTAAA 1 cut(s) 1182
Eam1104I CTCTTC 1 cut(s) 797
EarI CTCTTC 1 cut(s) 797
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 1 cut(s) 860
Eco24I GRGCYC 1 cut(s) 27
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 1180
Eco88I CYCGRG 2 cut(s) 248, 347
EcoICRI GAGCTC 1 cut(s) 25
EcoRII CCWGG 1 cut(s) 1205
EcoT14I CCWWGG 1 cut(s) 860
EcoT22I ATGCAT 2 cut(s) 396, 1168
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 860
Esp3I CGTCTC 1 cut(s) 1079
FaeI CATG 3 cut(s) 415, 970, 1170
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 2 cut(s) 293, 422
FatI CATG 3 cut(s) 411, 966, 1166
FbaI TGATCA 4 cut(s) 42, 112, 622, 978
Fnu4HI GCNGC 4 cut(s) 137, 273, 937, 1230
FokI GGATG 2 cut(s) 839, 1199
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 4 cut(s) 137, 273, 937, 1230
FspBI CTAG 2 cut(s) 509, 1303
GluI GCNGC 4 cut(s) 137, 273, 937, 1230
HaeIII GGCC 4 cut(s) 202, 679, 785, 1211
HapII CCGG 3 cut(s) 75, 786, 1156
Hin1II CATG 3 cut(s) 415, 970, 1170
HindIII AAGCTT 1 cut(s) 255
HpaII CCGG 3 cut(s) 75, 786, 1156
HphI GGTGA 2 cut(s) 191, 223
Hpy166II GTNNAC 1 cut(s) 1138
Hpy188I TCNGA 9 cut(s) 59, 90, 117, 220, 405, 622, 656, 852, 1251
Hpy8I GTNNAC 1 cut(s) 1138
Hpy99I CGWCG 1 cut(s) 660
HpyAV CCTTC 4 cut(s) 191, 644, 1231, 1276
HpyCH4III ACNGT 4 cut(s) 49, 67, 753, 1109
HpyCH4IV ACGT 2 cut(s) 658, 720
HpyF10VI GCNNNNNNNGC 4 cut(s) 208, 676, 1055, 1172
HpyF3I CTNAG 7 cut(s) 29, 147, 320, 483, 776, 851, 1050
HpySE526I ACGT 2 cut(s) 658, 720
Hsp92II CATG 3 cut(s) 415, 970, 1170
Kpn2I TCCGGA 1 cut(s) 74
KpnI GGTACC 2 cut(s) 40, 355
Ksp22I TGATCA 4 cut(s) 42, 112, 622, 978
LmnI GCTCC 3 cut(s) 22, 30, 60
Lsp1109I GCAGC 4 cut(s) 148, 284, 948, 1216
LweI GCATC 4 cut(s) 259, 306, 381, 1036
MaeI CTAG 2 cut(s) 509, 1303
MaeII ACGT 2 cut(s) 658, 720
MaeIII GTNAC 2 cut(s) 150, 766
MboII GAAGA 6 cut(s) 206, 814, 820, 851, 955, 1084
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 2 cut(s) 27, 634
MluCI AATT 4 cut(s) 188, 575, 833, 1023
MlyI GAGTC 3 cut(s) 224, 315, 590
MmeI TCCRAC 1 cut(s) 679
Mph1103I ATGCAT 2 cut(s) 396, 1168
MroI TCCGGA 1 cut(s) 74
MseI TTAA 6 cut(s) 339, 489, 533, 554, 1068, 1181
MslI CAYNNNNRTG 3 cut(s) 416, 965, 1014
MspI CCGG 3 cut(s) 75, 786, 1156
MspR9I CCNGG 2 cut(s) 787, 1207
Mva1269I GAATGC 2 cut(s) 394, 1168
MvaI CCWGG 1 cut(s) 1207
MwoI GCNNNNNNNGC 4 cut(s) 208, 676, 1055, 1172
NciI CCSGG 1 cut(s) 787
NheI GCTAGC 1 cut(s) 508
NlaIII CATG 3 cut(s) 415, 970, 1170
NlaIV GGNNCC 4 cut(s) 38, 353, 480, 631
NmeAIII GCCGAG 1 cut(s) 1099
NmuCI GTSAC 2 cut(s) 150, 766
NsiI ATGCAT 2 cut(s) 396, 1168
PaeR7I CTCGAG 1 cut(s) 248
PasI CCCWGGG 1 cut(s) 1206
PcsI WCGNNNNNNNCGW 1 cut(s) 395
PctI GAATGC 2 cut(s) 394, 1168
PfeI GAWTC 7 cut(s) 71, 118, 400, 427, 724, 757, 847
PflFI GACNNNGTC 1 cut(s) 660
PflMI CCANNNNNTGG 1 cut(s) 1087
PinAI ACCGGT 1 cut(s) 1155
PkrI GCNGC 4 cut(s) 138, 274, 938, 1231
PleI GAGTC 3 cut(s) 223, 314, 589
PpsI GAGTC 3 cut(s) 223, 314, 589
PshBI ATTAAT 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 1205
PspGI CCWGG 1 cut(s) 1205
PspN4I GGNNCC 4 cut(s) 38, 353, 480, 631
PspPI GGNCC 3 cut(s) 200, 677, 1209
PspXI VCTCGAGB 1 cut(s) 248
PstI CTGCAG 2 cut(s) 126, 471
PstNI CAGNNNCTG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 478
PsyI GACNNNGTC 1 cut(s) 660
RsaI GTAC 3 cut(s) 38, 353, 883
RsaNI GTAC 3 cut(s) 37, 352, 882
RseI CAYNNNNRTG 3 cut(s) 416, 965, 1014
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 6 cut(s) 339, 489, 533, 554, 1068, 1181
SatI GCNGC 4 cut(s) 137, 273, 937, 1230
Sau96I GGNCC 3 cut(s) 200, 677, 1209
SchI GAGTC 3 cut(s) 224, 315, 590
ScrFI CCNGG 2 cut(s) 787, 1207
SduI GDGCHC 2 cut(s) 27, 634
SfaNI GCATC 4 cut(s) 259, 306, 381, 1036
SfcI CTRYAG 4 cut(s) 122, 301, 467, 1288
Sfr274I CTCGAG 1 cut(s) 248
SgrAI CRCCGGYG 1 cut(s) 1155
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 3 cut(s) 416, 965, 1014
SmlI CTYRAG 3 cut(s) 248, 261, 422
SmoI CTYRAG 3 cut(s) 248, 261, 422
Sse9I AATT 4 cut(s) 188, 575, 833, 1023
SsiI CCGC 2 cut(s) 1141, 1295
SspMI CTAG 2 cut(s) 509, 1303
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 785, 1205
StyI CCWWGG 1 cut(s) 860
TaaI ACNGT 4 cut(s) 49, 67, 753, 1109
TaiI ACGT 2 cut(s) 661, 723
TaqI TCGA 6 cut(s) 249, 358, 389, 398, 525, 1095
TasI AATT 4 cut(s) 188, 575, 833, 1023
TfiI GAWTC 7 cut(s) 71, 118, 400, 427, 724, 757, 847
Tru1I TTAA 6 cut(s) 339, 489, 533, 554, 1068, 1181
Tru9I TTAA 6 cut(s) 339, 489, 533, 554, 1068, 1181
TscAI CASTG 4 cut(s) 594, 926, 1037, 1048
TseFI GTSAC 2 cut(s) 150, 766
TseI GCWGC 4 cut(s) 136, 272, 936, 1229
Tsp45I GTSAC 2 cut(s) 150, 766
TspDTI ATGAA 3 cut(s) 626, 749, 955
TspRI CASTG 4 cut(s) 594, 926, 1037, 1048
Tth111I GACNNNGTC 1 cut(s) 660
Van91I CCANNNNNTGG 1 cut(s) 1087
VspI ATTAAT 1 cut(s) 533
XapI RAATTY 2 cut(s) 188, 833
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XhoI CTCGAG 1 cut(s) 248
XspI CTAG 2 cut(s) 509, 1303
Zsp2I ATGCAT 2 cut(s) 396, 1168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.