Rh2BG197600

UPF0481 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0004633
Physical Location & Seq
Forward (+)
18053754 .. 18055340
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG197600.1

Sequence Viewer

Length: 1587 bp
ATGGTTGCTGTGTTCAACAAGGAGCTCCTGAGCTGGTACCTGATCACAGTCAAGCTCCGAGAAACAGTTGAATCCGGAATAGCAAATCCGAACCCATCAATCCAATCTCCTGATCAGAATCTGCAGAAACGAGAGCAGCAACAATCTCAGTCACTCCAAGTTGTGATCCAAAATGGTGGTGATGGGAAATTTGAAGAAGGGCCGAAATCGCCAGAGTCCGAGTGGGTTCTCACCATCAAAGATAAACTCGAGCAAGCTTCTCAAGACGATGCAGCAAATGTGTGGTCAAAGCTCTGCATCTACAGAGTCCCACATTACCTGAGAGAAGGTGATGACAAAGCTTATGTTCCTCAGATTGTGTCATTGGGGCCTTACCACCATGGAAGGAGACGCCTTCGCCAAATGGACCGCCACAAGTGGCGTTCGCTACATCAAATGCTCAAGAGGACTAATCAGGACATAAAGCTCTATCTGGATTCTATGAAAGAAGTTGAAGAAAAAGCTCGGTCTTGCTATGAAGGGCCGGTTGCAATAAGCCTTAGTAGCAATGAGTTTGTTGAAATGATGGTGCTTGATGGTTGCTTTGTGCTTGAGCTATTTCGTGGTGCAGCTGAGGGGTTTAAGCAACTCGGGTACCCTCGAAACGATCCCATTTTTGCAATGCGTGGCTCGATGCATTCGATTCAGAGGGACATGATTATGCTTGAGAATCAGCTTCCACTTTTCGTTCTTGATCGGCTATTGGGTCTGCAGTTGGGGGATCCTGAGCTTAAAGGACTTGTATCCAAGCTAGCATTGAGGTTTTTCGATCCATTAATGCCAACAGATGAACCATTAACAAAGAGTGATAGGAACAAATTGGAGTCATCACTGGGATATGCAACAACCTTTGATCCATTGTCTGATCAAGGTGCCCTTCATTGCCTTGATGTTTTCCGACGTAGTCTATTGCGATCAGGGCCGAAACCTGTGCCAAGAGTTTGGATCAAGAGATGGTCAAACGTGAATCGGGTTGCCGATAAAAGGAGGACACAGTTGATTCATTGTGTCACAGAGCTTAGGGAGGCCGGGATCAAGTTCAAGAAGAGGAAGACTGATCGGTTCTGGGATGTGAAATTTGAAAATGGGATTCTGAGTATTCCAAGGCTCTTGATCCACGATGGTACTAAATCTCTATTCCTCAATCTTATTGCATTTGAGCAGTGTCATCTTGATTGCAGCAATGACATTACCTCCTATGTCATCTTCATGGACAACTTGATCAACTCTCCTGCTGATGTGGGTTATCTACATTATTGTGGCATAATTGAACACTGGCTTGGTAGTGATGCTGAGGTTGCTGACCTATTTAATCGTCTCTGCCAAGAGGTGGTCTTCGACATCAATGACAGTTATCTTTCTCGGCTTTCGGCTGATGTAAACCGCTACTACGACCACCGGTGGAATGCATGGCGTGCCAGTTTAAAGCACAACTACTTCAGCAATCCCTGGGCCATCCTCTCCTTCCTTGCTGCTGTTGTGTTGCTGTTTCTGACTTTCACTCAAACCTTCTATGGAGTTTATAGCTACTATAGACCGCCAAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

528

Amino Acids

61.1

Weight (kDa)

7.98

Isoelectric Point (pI)

47.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 100 - 511 3.2e-136 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 36, 633
AccB1I GGYRCC 3 cut(s) 36, 633, 911
AccB7I CCANNNNNTGG 1 cut(s) 1369
AccIII TCCGGA 1 cut(s) 74
AciI CCGC 3 cut(s) 409, 1423, 1577
AclWI GGATC 9 cut(s) 160, 641, 755, 768, 803, 887, 992, 1079, 1147
AcsI RAATTY 2 cut(s) 188, 1115
AcuI CTGAAG 1 cut(s) 1462
AcyI GRCGYC 1 cut(s) 391
AfaI GTAC 3 cut(s) 38, 635, 1165
AfiI CCNNNNNNNGG 2 cut(s) 1023, 1369
AgeI ACCGGT 1 cut(s) 1437
AgsI TTSAA 8 cut(s) 16, 71, 194, 494, 560, 1081, 1121, 1310
AjnI CCWGG 1 cut(s) 1487
AjuI GAANNNNNNNTTGG 2 cut(s) 1302, 1334
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 382, 1361
AlwI GGATC 9 cut(s) 160, 641, 755, 768, 803, 887, 992, 1079, 1147
AlwNI CAGNNNCTG 1 cut(s) 121
Ama87I CYCGRG 2 cut(s) 248, 629
Aor13HI TCCGGA 1 cut(s) 74
AoxI GGCC 6 cut(s) 200, 368, 521, 959, 1065, 1491
ApeKI GCWGC 5 cut(s) 136, 272, 608, 1218, 1511
ApoI RAATTY 2 cut(s) 188, 1115
ArsI GACNNNNNNTTYG 2 cut(s) 269, 301
AseI ATTAAT 1 cut(s) 815
AsiGI ACCGGT 1 cut(s) 1437
Asp718I GGTACC 2 cut(s) 36, 633
AspS9I GGNCC 6 cut(s) 200, 368, 406, 521, 959, 1491
AsuC2I CCSGG 1 cut(s) 1069
AsuHPI GGTGA 3 cut(s) 191, 223, 341
AsuNHI GCTAGC 1 cut(s) 790
AvaI CYCGRG 2 cut(s) 248, 629
AvaII GGWCC 1 cut(s) 406
BaeGI GKGCMC 1 cut(s) 916
BaeI ACNNNNGTAYC 2 cut(s) 617, 650
BamHI GGATCC 1 cut(s) 760
BanI GGYRCC 3 cut(s) 36, 633, 911
BanII GRGCYC 1 cut(s) 27
BbsI GAAGAC 2 cut(s) 1097, 1366
Bbv12I GWGCWC 1 cut(s) 27
BbvCI CCTCAGC 2 cut(s) 612, 1332
BbvI GCAGC 5 cut(s) 148, 284, 620, 1230, 1498
BccI CCATC 8 cut(s) 103, 176, 242, 559, 569, 987, 1154, 1502
BcgI CGANNNNNNTGC 2 cut(s) 952, 986
BciT130I CCWGG 1 cut(s) 1489
BciVI GTATCC 1 cut(s) 793
BclI TGATCA 4 cut(s) 42, 112, 904, 1260
BcnI CCSGG 1 cut(s) 1069
BcoDI GTCTC 2 cut(s) 382, 1361
BfaI CTAG 2 cut(s) 791, 1585
BfmI CTRYAG 4 cut(s) 122, 301, 749, 1570
BfuI GTATCC 1 cut(s) 793
BisI GCNGC 5 cut(s) 137, 273, 609, 1219, 1512
BlsI GCNGC 5 cut(s) 138, 274, 610, 1220, 1513
Bme1390I CCNGG 2 cut(s) 1069, 1489
Bme18I GGWCC 1 cut(s) 406
BmeT110I CYCGRG 2 cut(s) 248, 629
BmgT120I GGNCC 6 cut(s) 200, 368, 406, 521, 959, 1491
BmiI GGNNCC 5 cut(s) 38, 369, 635, 762, 913
BmrFI CCNGG 2 cut(s) 1069, 1489
BmrI ACTGGG 1 cut(s) 881
BmsI GCATC 4 cut(s) 259, 306, 663, 1318
BmtI GCTAGC 1 cut(s) 794
BmuI ACTGGG 1 cut(s) 881
BpiI GAAGAC 2 cut(s) 1097, 1366
Bpu10I CCTNAGC 5 cut(s) 29, 612, 765, 1058, 1332
BpuEI CTTGAG 4 cut(s) 246, 425, 611, 725
BpuMI CCSGG 1 cut(s) 1069
BsaBI GATNNNNATC 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 391
BsaJI CCNNGG 4 cut(s) 379, 1142, 1487, 1488
BsaWI WCCGGW 2 cut(s) 74, 1437
BsaXI ACNNNNNCTCC 2 cut(s) 1217, 1247
Bsc4I CCNNNNNNNGG 2 cut(s) 1023, 1369
Bse118I RCCGGY 2 cut(s) 523, 1437
Bse1I ACTGG 3 cut(s) 876, 1319, 1458
Bse3DI GCAATG 4 cut(s) 553, 666, 919, 1228
Bse8I GATNNNNATC 1 cut(s) 117
BseAI TCCGGA 1 cut(s) 74
BseBI CCWGG 1 cut(s) 1489
BseDI CCNNGG 4 cut(s) 379, 1142, 1487, 1488
BseGI GGATG 2 cut(s) 1114, 1494
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 2 cut(s) 1023, 1369
BseMI GCAATG 4 cut(s) 553, 666, 919, 1228
BseMII CTCAG 8 cut(s) 20, 161, 311, 365, 603, 756, 1124, 1323
BseNI ACTGG 3 cut(s) 876, 1319, 1458
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 5 cut(s) 148, 284, 620, 1230, 1498
BsgI GTGCAG 1 cut(s) 627
BshFI GGCC 6 cut(s) 202, 370, 523, 961, 1067, 1493
BshNI GGYRCC 3 cut(s) 36, 633, 911
BshTI ACCGGT 1 cut(s) 1437
BsiHKAI GWGCWC 1 cut(s) 27
BsiHKCI CYCGRG 2 cut(s) 248, 629
BsiSI CCGG 4 cut(s) 75, 524, 1068, 1438
BslFI GGGAC 2 cut(s) 293, 704
BslI CCNNNNNNNGG 2 cut(s) 1023, 1369
BsmAI GTCTC 2 cut(s) 382, 1361
BsmBI CGTCTC 2 cut(s) 382, 1361
BsmFI GGGAC 2 cut(s) 293, 704
BsmI GAATGC 2 cut(s) 676, 1450
BsnI GGCC 6 cut(s) 202, 370, 523, 961, 1067, 1493
BsoBI CYCGRG 2 cut(s) 248, 629
Bsp1286I GDGCHC 2 cut(s) 27, 916
Bsp13I TCCGGA 1 cut(s) 74
Bsp19I CCATGG 1 cut(s) 379
BspACI CCGC 3 cut(s) 409, 1423, 1577
BspANI GGCC 6 cut(s) 202, 370, 523, 961, 1067, 1493
BspCNI CTCAG 8 cut(s) 21, 160, 312, 364, 604, 757, 1125, 1324
BspEI TCCGGA 1 cut(s) 74
BspLI GGNNCC 5 cut(s) 38, 369, 635, 762, 913
BspMAI CTGCAG 2 cut(s) 126, 753
BspOI GCTAGC 1 cut(s) 794
BspPI GGATC 9 cut(s) 160, 641, 755, 768, 803, 887, 992, 1079, 1147
BspT107I GGYRCC 3 cut(s) 36, 633, 911
BsrDI GCAATG 4 cut(s) 553, 666, 919, 1228
BsrFI RCCGGY 2 cut(s) 523, 1437
BsrI ACTGG 3 cut(s) 876, 1319, 1458
BssAI RCCGGY 2 cut(s) 523, 1437
BssECI CCNNGG 4 cut(s) 379, 1142, 1487, 1488
BssNI GRCGYC 1 cut(s) 391
BssT1I CCWWGG 2 cut(s) 379, 1142
Bst2UI CCWGG 1 cut(s) 1489
Bst4CI ACNGT 4 cut(s) 49, 67, 1035, 1391
Bst6I CTCTTC 1 cut(s) 1079
BstACI GRCGYC 1 cut(s) 391
BstAPI GCANNNNNTGC 1 cut(s) 1454
BstC8I GCNNGC 3 cut(s) 255, 792, 1455
BstDSI CCRYGG 1 cut(s) 379
BstF5I GGATG 2 cut(s) 1114, 1494
BstMAI GTCTC 2 cut(s) 382, 1361
BstMWI GCNNNNNNNGC 5 cut(s) 208, 543, 958, 1337, 1454
BstNI CCWGG 1 cut(s) 1489
BstSCI CCNGG 2 cut(s) 1067, 1487
BstSFI CTRYAG 4 cut(s) 122, 301, 749, 1570
BstSLI GKGCMC 1 cut(s) 916
BstV1I GCAGC 5 cut(s) 148, 284, 620, 1230, 1498
BstV2I GAAGAC 2 cut(s) 1097, 1366
BstX2I RGATCY 1 cut(s) 760
BstXI CCANNNNNNTGG 2 cut(s) 176, 981
BstYI RGATCY 1 cut(s) 760
BsuI GTATCC 1 cut(s) 793
BsuRI GGCC 6 cut(s) 202, 370, 523, 961, 1067, 1493
BtgI CCRYGG 1 cut(s) 379
BtsCI GGATG 2 cut(s) 1114, 1494
BtsI GCAGTG 1 cut(s) 1208
BtsIMutI CAGTG 3 cut(s) 869, 1208, 1312
Cac8I GCNNGC 3 cut(s) 255, 792, 1455
CaiI CAGNNNCTG 1 cut(s) 121
Cfr10I RCCGGY 2 cut(s) 523, 1437
Cfr13I GGNCC 6 cut(s) 200, 368, 406, 521, 959, 1491
CseI GACGC 1 cut(s) 399
Csp6I GTAC 3 cut(s) 37, 634, 1164
CspAI ACCGGT 1 cut(s) 1437
CviAII CATG 4 cut(s) 380, 694, 1249, 1449
CviQI GTAC 3 cut(s) 37, 634, 1164
DraI TTTAAA 1 cut(s) 1464
Eam1104I CTCTTC 1 cut(s) 1079
EarI CTCTTC 1 cut(s) 1079
Ecl136II GAGCTC 1 cut(s) 25
Eco130I CCWWGG 2 cut(s) 379, 1142
Eco24I GRGCYC 1 cut(s) 27
Eco47I GGWCC 1 cut(s) 406
Eco53kI GAGCTC 1 cut(s) 25
Eco57I CTGAAG 1 cut(s) 1462
Eco88I CYCGRG 2 cut(s) 248, 629
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 1487
EcoT14I CCWWGG 2 cut(s) 379, 1142
EcoT22I ATGCAT 2 cut(s) 678, 1450
EcoT38I GRGCYC 1 cut(s) 27
ErhI CCWWGG 2 cut(s) 379, 1142
Esp3I CGTCTC 2 cut(s) 382, 1361
FaeI CATG 4 cut(s) 383, 697, 1252, 1452
FalI AAGNNNNNCTT 2 cut(s) 900, 932
FaqI GGGAC 2 cut(s) 293, 704
FatI CATG 4 cut(s) 379, 693, 1248, 1448
FbaI TGATCA 4 cut(s) 42, 112, 904, 1260
Fnu4HI GCNGC 5 cut(s) 137, 273, 609, 1219, 1512
FokI GGATG 2 cut(s) 1121, 1481
FriOI GRGCYC 1 cut(s) 27
Fsp4HI GCNGC 5 cut(s) 137, 273, 609, 1219, 1512
FspBI CTAG 2 cut(s) 791, 1585
GluI GCNGC 5 cut(s) 137, 273, 609, 1219, 1512
HaeIII GGCC 6 cut(s) 202, 370, 523, 961, 1067, 1493
HapII CCGG 4 cut(s) 75, 524, 1068, 1438
HgaI GACGC 1 cut(s) 399
Hin1I GRCGYC 1 cut(s) 391
Hin1II CATG 4 cut(s) 383, 697, 1252, 1452
HindIII AAGCTT 2 cut(s) 255, 339
HpaII CCGG 4 cut(s) 75, 524, 1068, 1438
HphI GGTGA 3 cut(s) 191, 223, 341
Hpy166II GTNNAC 1 cut(s) 1420
Hpy8I GTNNAC 1 cut(s) 1420
Hpy99I CGWCG 1 cut(s) 942
HpyAV CCTTC 8 cut(s) 191, 320, 378, 404, 512, 926, 1513, 1558
HpyCH4III ACNGT 4 cut(s) 49, 67, 1035, 1391
HpyCH4IV ACGT 2 cut(s) 940, 1002
HpyF10VI GCNNNNNNNGC 5 cut(s) 208, 543, 958, 1337, 1454
HpySE526I ACGT 2 cut(s) 940, 1002
Hsp92I GRCGYC 1 cut(s) 391
Hsp92II CATG 4 cut(s) 383, 697, 1252, 1452
Kpn2I TCCGGA 1 cut(s) 74
KpnI GGTACC 2 cut(s) 40, 637
Ksp22I TGATCA 4 cut(s) 42, 112, 904, 1260
LmnI GCTCC 3 cut(s) 22, 30, 60
Lsp1109I GCAGC 5 cut(s) 148, 284, 620, 1230, 1498
LweI GCATC 4 cut(s) 259, 306, 663, 1318
MaeI CTAG 2 cut(s) 791, 1585
MaeII ACGT 2 cut(s) 940, 1002
MaeIII GTNAC 2 cut(s) 150, 1048
MboII GAAGA 6 cut(s) 206, 506, 1096, 1102, 1237, 1366
MflI RGATCY 1 cut(s) 760
MhlI GDGCHC 2 cut(s) 27, 916
MluCI AATT 4 cut(s) 188, 857, 1115, 1305
MlyI GAGTC 3 cut(s) 224, 315, 872
MmeI TCCRAC 1 cut(s) 961
Mph1103I ATGCAT 2 cut(s) 678, 1450
MroI TCCGGA 1 cut(s) 74
MseI TTAA 6 cut(s) 621, 771, 815, 836, 1350, 1463
MslI CAYNNNNRTG 3 cut(s) 698, 1247, 1296
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 4 cut(s) 75, 524, 1068, 1438
MspR9I CCNGG 2 cut(s) 1069, 1489
Mva1269I GAATGC 2 cut(s) 676, 1450
MvaI CCWGG 1 cut(s) 1489
MwoI GCNNNNNNNGC 5 cut(s) 208, 543, 958, 1337, 1454
NciI CCSGG 1 cut(s) 1069
NcoI CCATGG 1 cut(s) 379
NheI GCTAGC 1 cut(s) 790
NlaIII CATG 4 cut(s) 383, 697, 1252, 1452
NlaIV GGNNCC 5 cut(s) 38, 369, 635, 762, 913
NmeAIII GCCGAG 1 cut(s) 1381
NmuCI GTSAC 2 cut(s) 150, 1048
NsiI ATGCAT 2 cut(s) 678, 1450
PaeR7I CTCGAG 1 cut(s) 248
PasI CCCWGGG 1 cut(s) 1488
PcsI WCGNNNNNNNCGW 1 cut(s) 677
PctI GAATGC 2 cut(s) 676, 1450
PfeI GAWTC 8 cut(s) 71, 118, 476, 682, 709, 1006, 1039, 1129
PflFI GACNNNGTC 1 cut(s) 942
PflMI CCANNNNNTGG 1 cut(s) 1369
PinAI ACCGGT 1 cut(s) 1437
PkrI GCNGC 5 cut(s) 138, 274, 610, 1220, 1513
PleI GAGTC 3 cut(s) 223, 314, 871
PpsI GAGTC 3 cut(s) 223, 314, 871
PshBI ATTAAT 1 cut(s) 815
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 1487
PspGI CCWGG 1 cut(s) 1487
PspN4I GGNNCC 5 cut(s) 38, 369, 635, 762, 913
PspPI GGNCC 6 cut(s) 200, 368, 406, 521, 959, 1491
PspXI VCTCGAGB 1 cut(s) 248
PstI CTGCAG 2 cut(s) 126, 753
PstNI CAGNNNCTG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 760
PsyI GACNNNGTC 1 cut(s) 942
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 3 cut(s) 38, 635, 1165
RsaNI GTAC 3 cut(s) 37, 634, 1164
RseI CAYNNNNRTG 3 cut(s) 698, 1247, 1296
SacI GAGCTC 1 cut(s) 27
SaqAI TTAA 6 cut(s) 621, 771, 815, 836, 1350, 1463
SatI GCNGC 5 cut(s) 137, 273, 609, 1219, 1512
Sau96I GGNCC 6 cut(s) 200, 368, 406, 521, 959, 1491
SchI GAGTC 3 cut(s) 224, 315, 872
ScrFI CCNGG 2 cut(s) 1069, 1489
SduI GDGCHC 2 cut(s) 27, 916
SfaNI GCATC 4 cut(s) 259, 306, 663, 1318
SfcI CTRYAG 4 cut(s) 122, 301, 749, 1570
Sfr274I CTCGAG 1 cut(s) 248
SgrAI CRCCGGYG 1 cut(s) 1437
SinI GGWCC 1 cut(s) 406
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 3 cut(s) 698, 1247, 1296
SmlI CTYRAG 5 cut(s) 248, 261, 440, 590, 704
SmoI CTYRAG 5 cut(s) 248, 261, 440, 590, 704
Sse9I AATT 4 cut(s) 188, 857, 1115, 1305
SsiI CCGC 3 cut(s) 409, 1423, 1577
SspMI CTAG 2 cut(s) 791, 1585
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 1067, 1487
StyI CCWWGG 2 cut(s) 379, 1142
TaaI ACNGT 4 cut(s) 49, 67, 1035, 1391
TaiI ACGT 2 cut(s) 943, 1005
TaqI TCGA 6 cut(s) 249, 640, 671, 680, 807, 1377
TaqII GACCGA 1 cut(s) 495
TasI AATT 4 cut(s) 188, 857, 1115, 1305
TfiI GAWTC 8 cut(s) 71, 118, 476, 682, 709, 1006, 1039, 1129
Tru1I TTAA 6 cut(s) 621, 771, 815, 836, 1350, 1463
Tru9I TTAA 6 cut(s) 621, 771, 815, 836, 1350, 1463
TscAI CASTG 3 cut(s) 876, 1208, 1319
TseFI GTSAC 2 cut(s) 150, 1048
TseI GCWGC 5 cut(s) 136, 272, 608, 1218, 1511
Tsp45I GTSAC 2 cut(s) 150, 1048
TspDTI ATGAA 6 cut(s) 497, 531, 843, 908, 1031, 1237
TspRI CASTG 3 cut(s) 876, 1208, 1319
Tth111I GACNNNGTC 1 cut(s) 942
Van91I CCANNNNNTGG 1 cut(s) 1369
VpaK11BI GGWCC 1 cut(s) 406
VspI ATTAAT 1 cut(s) 815
XapI RAATTY 2 cut(s) 188, 1115
XcmI CCANNNNNNNNNTGG 1 cut(s) 219
XhoI CTCGAG 1 cut(s) 248
XspI CTAG 2 cut(s) 791, 1585
Zsp2I ATGCAT 2 cut(s) 678, 1450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.