FvH4_1g25480

Dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010272
Physical Location & Seq
Reverse (-)
17223340 .. 17230203
6864 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25480.t1

Sequence Viewer

Length: 1158 bp
ATGTTTTCAAGGGTGAGCTTCCGTTTGCGAGCCCTTGTGTGGCGGGACATGAGATTACTGGGGAGGTTGTCGAACATGGACCCGATACGGATAACAAGGTCACTCAAAGACTGTGTAGTGACTGATAACTATTTGACATGCTTGGTGCACGAGGGGAGAGCATTCGGTCTAGATATGGAGTTGGGACTTATAAACTCAAAATGCAATTCAGTGGAGCGAGATTGGGAATCAGGAATTGAGGATGGTAGAGGTCTTTTGGGAATCGAAGGTCTAAGATTACCGATTGGATCTCGTGTTGTTGGAGCTTTTATCATGCCTTGTGGTAGCTGTTCCTACTGTTCAAAGGGCCACGACGATTTATGCGAGGATTTCTTTGCCTACAACCGTGCCAAAGGAACCCTTTATGATGGTGAAACTCGGTTATTCCTCCGCAGCAGTGGAAAACCCGTATACATGTATAGTATGGGAGGCCTTGCTGAGTACTGTGTTGTACCAGCAAATGCATTGACTATTTTACCGAAGTCATTGCCGTATACAGAATCTGCCATTTTAGGATGTGCAGTATTTACTGCTTATGGTGCTATGGCACATGCAGCTGAGGTTCGTCCTGGGGATTCAGTTGCTATTATTGGAACTGGGGGCGTTGGATCCAGCTGTCTGCAGATAGCCAGAGCATTTGGTGCCTCCGATATTATTGCGGTCGATATTCAAGACGACAAACTGCAGAAAGCCAAGATATTTGGTGCTACTCATGTTATAAATGCGGCTAAAGAAAATGCTGTTGATAAAATATTGGAAATTACTGGTGGGAAAGGTGTGGACGTTGCTGTGGAAGCCCTGGGAAAACCACAGACCTTTTTGCAATGCACGCAAAGTGTGAGAGATGGAGGAAAAGCTGTCTTCATTGGGCTTACAGCAGTTGGTGTAATTGGGGAAGTGGATATTAACCGTCTTGTTCGTAGAAAGATACAAATAATTGGATCATACGGAGGAAGGGCAAGGCAGGATCTTCCAAAGATAGTTAGACTAGCAGAGAGAGGTATATTTGATCTCTCTAGTGCTGTTTCAAGAAGATATAAATTTGAGGAGTCTGCCAGTGCATACCAGGATCTCAACCAGGGAAGCATTGTTGGCCGTGCTGTGGTTGAGATAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ADH_N ADH_zinc_N

Protein Analysis

386

Amino Acids

41.63

Weight (kDa)

8.42

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ADH_N PF08240 92 - 173 1e-06 Alcohol dehydrogenase GroES-like domain
Glu_dehyd_C PF16912 195 - 372 9.4e-07 Glucose dehydrogenase C-terminus
ADH_zinc_N PF00107 214 - 345 2.9e-32 Zinc-binding dehydrogenase
ADH_zinc_N_2 PF13602 247 - 382 5.3e-13 Zinc-binding dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010272)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G63620 AT5G63620 AT5G63620
fragaria_vesca FvH4_1g25480 FvH4_1g25480 FvH4_1g25480 FvH4_1g25480
malus_domestica MD00G1129200.v1.1
prunus_persica Prupe.4G281800_v2.0.a1
pyrus_communis pycom520g00640
rosa_chinensis RchiOBHm_Chr2g0123611
rosa_laevigata RLG00000018760
rosa_multiflora Rmu_sc0005477.1_g000001 Rmu_ssc0000091.1_g000016
rosa_roxburghii Rroxscaffold_2G00120540
rosa_rugosa Rorug02G0246400
rosa_samantha Rh2AG305700 Rh2BG313600 Rh2CG291800 Rh2DG329500
rosa_wichuraiana Rw2G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 191, 758
AccB1I GGYRCC 1 cut(s) 680
AccI GTMKAC 2 cut(s) 450, 533
AciI CCGC 4 cut(s) 43, 430, 698, 764
AclWI GGATC 6 cut(s) 295, 642, 655, 988, 1014, 1116
AcoI YGGCCR 1 cut(s) 1132
AcsI RAATTY 1 cut(s) 1079
AfaI GTAC 2 cut(s) 482, 492
AfiI CCNNNNNNNGG 2 cut(s) 39, 1141
AflIII ACRYGT 1 cut(s) 453
AgsI TTSAA 4 cut(s) 9, 342, 710, 1068
AjnI CCWGG 4 cut(s) 607, 837, 1104, 1116
AluBI AGCT 6 cut(s) 18, 305, 327, 596, 654, 896
AluI AGCT 6 cut(s) 18, 305, 327, 596, 654, 896
Alw21I GWGCWC 1 cut(s) 150
Alw44I GTGCAC 1 cut(s) 146
AlwI GGATC 6 cut(s) 295, 642, 655, 988, 1014, 1116
AlwNI CAGNNNCTG 1 cut(s) 542
AoxI GGCC 3 cut(s) 346, 469, 1132
ApaLI GTGCAC 1 cut(s) 146
ApeKI GCWGC 2 cut(s) 432, 593
ApoI RAATTY 1 cut(s) 1079
AspS9I GGNCC 2 cut(s) 79, 346
AsuHPI GGTGA 2 cut(s) 25, 422
AvaII GGWCC 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 150
BamHI GGATCC 1 cut(s) 647
BanI GGYRCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 34
BauI CACGAG 2 cut(s) 149, 291
BbsI GAAGAC 1 cut(s) 892
Bbv12I GWGCWC 1 cut(s) 150
BbvCI CCTCAGC 1 cut(s) 597
BbvI GCAGC 2 cut(s) 444, 605
BccI CCATC 3 cut(s) 236, 401, 878
BceAI ACGGC 2 cut(s) 514, 1119
BcgI CGANNNNNNTGC 4 cut(s) 508, 542, 677, 711
BciT130I CCWGG 4 cut(s) 609, 839, 1106, 1118
BfaI CTAG 3 cut(s) 170, 1028, 1056
BfmI CTRYAG 2 cut(s) 659, 722
BisI GCNGC 3 cut(s) 433, 594, 765
BlsI GCNGC 3 cut(s) 434, 595, 766
BmcAI AGTACT 1 cut(s) 482
Bme1390I CCNGG 4 cut(s) 609, 839, 1106, 1118
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 2 cut(s) 79, 346
BmiI GGNNCC 4 cut(s) 81, 397, 649, 682
BmrFI CCNGG 4 cut(s) 609, 839, 1106, 1118
BmrI ACTGGG 2 cut(s) 68, 645
BmuI ACTGGG 2 cut(s) 68, 645
BpiI GAAGAC 1 cut(s) 892
Bpu10I CCTNAGC 1 cut(s) 597
BsaJI CCNNGG 4 cut(s) 608, 837, 838, 1117
Bsc4I CCNNNNNNNGG 2 cut(s) 39, 1141
Bse1I ACTGG 4 cut(s) 63, 640, 808, 1095
Bse3DI GCAATG 2 cut(s) 524, 869
BseBI CCWGG 4 cut(s) 609, 839, 1106, 1118
BseDI CCNNGG 4 cut(s) 608, 837, 838, 1117
BseGI GGATG 2 cut(s) 247, 560
BseLI CCNNNNNNNGG 2 cut(s) 39, 1141
BseMI GCAATG 2 cut(s) 524, 869
BseMII CTCAG 2 cut(s) 468, 588
BseNI ACTGG 4 cut(s) 63, 640, 808, 1095
BseRI GAGGAG 1 cut(s) 1100
BseSI GKGCMC 1 cut(s) 150
BseXI GCAGC 2 cut(s) 444, 605
BsgI GTGCAG 1 cut(s) 579
Bsh1285I CGRYCG 1 cut(s) 702
BshFI GGCC 3 cut(s) 348, 471, 1134
BshNI GGYRCC 1 cut(s) 680
BsiEI CGRYCG 1 cut(s) 702
BsiHKAI GWGCWC 1 cut(s) 150
BslFI GGGAC 2 cut(s) 59, 198
BslI CCNNNNNNNGG 2 cut(s) 39, 1141
BsmFI GGGAC 2 cut(s) 59, 198
BsmI GAATGC 1 cut(s) 161
BsnI GGCC 3 cut(s) 348, 471, 1134
Bsp1286I GDGCHC 2 cut(s) 34, 150
Bsp143I GATC 6 cut(s) 287, 647, 980, 1006, 1048, 1108
BspACI CCGC 4 cut(s) 43, 430, 698, 764
BspANI GGCC 3 cut(s) 348, 471, 1134
BspCNI CTCAG 2 cut(s) 469, 589
BspLI GGNNCC 4 cut(s) 81, 397, 649, 682
BspMAI CTGCAG 2 cut(s) 663, 726
BspPI GGATC 6 cut(s) 295, 642, 655, 988, 1014, 1116
BspT107I GGYRCC 1 cut(s) 680
BsrDI GCAATG 2 cut(s) 524, 869
BsrI ACTGG 4 cut(s) 63, 640, 808, 1095
BssECI CCNNGG 4 cut(s) 608, 837, 838, 1117
BssMI GATC 6 cut(s) 287, 647, 980, 1006, 1048, 1108
BssNAI GTATAC 2 cut(s) 451, 534
BssSI CACGAG 2 cut(s) 149, 291
Bst1107I GTATAC 2 cut(s) 451, 534
Bst2BI CACGAG 2 cut(s) 149, 291
Bst2UI CCWGG 4 cut(s) 609, 839, 1106, 1118
Bst4CI ACNGT 5 cut(s) 113, 338, 386, 485, 950
BstAPI GCANNNNNTGC 1 cut(s) 680
BstC8I GCNNGC 2 cut(s) 30, 869
BstDEI CTNAG 3 cut(s) 272, 477, 597
BstF5I GGATG 2 cut(s) 247, 560
BstKTI GATC 6 cut(s) 290, 650, 983, 1009, 1051, 1111
BstMBI GATC 6 cut(s) 287, 647, 980, 1006, 1048, 1108
BstMCI CGRYCG 1 cut(s) 702
BstMWI GCNNNNNNNGC 6 cut(s) 578, 593, 680, 833, 868, 1131
BstNI CCWGG 4 cut(s) 609, 839, 1106, 1118
BstNSI RCATGY 3 cut(s) 141, 457, 593
BstSCI CCNGG 4 cut(s) 607, 837, 1104, 1116
BstSFI CTRYAG 2 cut(s) 659, 722
BstSLI GKGCMC 1 cut(s) 150
BstV1I GCAGC 2 cut(s) 444, 605
BstV2I GAAGAC 1 cut(s) 892
BstX2I RGATCY 4 cut(s) 287, 647, 1006, 1108
BstYI RGATCY 4 cut(s) 287, 647, 1006, 1108
BstZ17I GTATAC 2 cut(s) 451, 534
BsuRI GGCC 3 cut(s) 348, 471, 1134
BtsCI GGATG 2 cut(s) 247, 560
BtsI GCAGTG 1 cut(s) 442
BtsIMutI CAGTG 3 cut(s) 216, 442, 1102
Cac8I GCNNGC 2 cut(s) 30, 869
CaiI CAGNNNCTG 1 cut(s) 542
Cfr13I GGNCC 2 cut(s) 79, 346
Csp6I GTAC 2 cut(s) 481, 491
CviAII CATG 7 cut(s) 49, 76, 138, 313, 454, 590, 752
CviQI GTAC 2 cut(s) 481, 491
DdeI CTNAG 3 cut(s) 272, 477, 597
DpnI GATC 6 cut(s) 289, 649, 982, 1008, 1050, 1110
DpnII GATC 6 cut(s) 287, 647, 980, 1006, 1048, 1108
EaeI YGGCCR 1 cut(s) 1132
Eco147I AGGCCT 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 79
EcoRII CCWGG 4 cut(s) 607, 837, 1104, 1116
EcoT22I ATGCAT 1 cut(s) 505
EcoT38I GRGCYC 1 cut(s) 34
FaeI CATG 7 cut(s) 52, 79, 141, 316, 457, 593, 755
FalI AAGNNNNNCTT 2 cut(s) 384, 416
FaqI GGGAC 2 cut(s) 59, 198
FatI CATG 7 cut(s) 48, 75, 137, 312, 453, 589, 751
FauI CCCGC 1 cut(s) 36
FblI GTMKAC 2 cut(s) 450, 533
Fnu4HI GCNGC 3 cut(s) 433, 594, 765
FokI GGATG 2 cut(s) 254, 567
FriOI GRGCYC 1 cut(s) 34
Fsp4HI GCNGC 3 cut(s) 433, 594, 765
FspBI CTAG 3 cut(s) 170, 1028, 1056
GluI GCNGC 3 cut(s) 433, 594, 765
HaeIII GGCC 3 cut(s) 348, 471, 1134
Hin1II CATG 7 cut(s) 52, 79, 141, 316, 457, 593, 755
HinfI GANTC 5 cut(s) 227, 261, 539, 614, 1088
HphI GGTGA 2 cut(s) 25, 422
Hpy166II GTNNAC 4 cut(s) 148, 451, 534, 820
Hpy188I TCNGA 1 cut(s) 688
Hpy188III TCNNGA 4 cut(s) 170, 231, 710, 1068
Hpy8I GTNNAC 4 cut(s) 148, 451, 534, 820
Hpy99I CGWCG 1 cut(s) 356
HpyAV CCTTC 2 cut(s) 260, 987
HpyCH4III ACNGT 5 cut(s) 113, 338, 386, 485, 950
HpyCH4IV ACGT 1 cut(s) 822
HpyF10VI GCNNNNNNNGC 6 cut(s) 578, 593, 680, 833, 868, 1131
HpyF3I CTNAG 3 cut(s) 272, 477, 597
HpySE526I ACGT 1 cut(s) 822
Hsp92II CATG 7 cut(s) 52, 79, 141, 316, 457, 593, 755
Kzo9I GATC 6 cut(s) 287, 647, 980, 1006, 1048, 1108
LmnI GCTCC 2 cut(s) 214, 302
Lsp1109I GCAGC 2 cut(s) 444, 605
MaeI CTAG 3 cut(s) 170, 1028, 1056
MaeII ACGT 1 cut(s) 822
MaeIII GTNAC 2 cut(s) 99, 118
MalI GATC 6 cut(s) 289, 649, 982, 1008, 1050, 1110
MboI GATC 6 cut(s) 287, 647, 980, 1006, 1048, 1108
MboII GAAGA 3 cut(s) 892, 1001, 1083
MflI RGATCY 4 cut(s) 287, 647, 1006, 1108
MhlI GDGCHC 2 cut(s) 34, 150
MluCI AATT 6 cut(s) 205, 234, 798, 927, 975, 1079
MlyI GAGTC 1 cut(s) 1097
MmeI TCCRAC 2 cut(s) 280, 625
Mph1103I ATGCAT 1 cut(s) 505
MseI TTAA 1 cut(s) 945
MspA1I CMGCKG 2 cut(s) 596, 654
MspR9I CCNGG 4 cut(s) 609, 839, 1106, 1118
Mva1269I GAATGC 1 cut(s) 161
MvaI CCWGG 4 cut(s) 609, 839, 1106, 1118
MwoI GCNNNNNNNGC 6 cut(s) 578, 593, 680, 833, 868, 1131
NdeII GATC 6 cut(s) 287, 647, 980, 1006, 1048, 1108
NlaIII CATG 7 cut(s) 52, 79, 141, 316, 457, 593, 755
NlaIV GGNNCC 4 cut(s) 81, 397, 649, 682
NmuCI GTSAC 2 cut(s) 99, 118
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 3 cut(s) 141, 457, 593
PasI CCCWGGG 1 cut(s) 838
PceI AGGCCT 1 cut(s) 471
PciI ACATGT 1 cut(s) 453
PcsI WCGNNNNNNNCGW 1 cut(s) 360
PctI GAATGC 1 cut(s) 161
PfeI GAWTC 4 cut(s) 227, 261, 539, 614
PkrI GCNGC 3 cut(s) 434, 595, 766
PleI GAGTC 1 cut(s) 1096
PpsI GAGTC 1 cut(s) 1096
PscI ACATGT 1 cut(s) 453
PsiI TTATAA 2 cut(s) 191, 758
Psp6I CCWGG 4 cut(s) 607, 837, 1104, 1116
PspGI CCWGG 4 cut(s) 607, 837, 1104, 1116
PspN4I GGNNCC 4 cut(s) 81, 397, 649, 682
PspPI GGNCC 2 cut(s) 79, 346
PstI CTGCAG 2 cut(s) 663, 726
PstNI CAGNNNCTG 1 cut(s) 542
PsuI RGATCY 4 cut(s) 287, 647, 1006, 1108
PvuII CAGCTG 2 cut(s) 596, 654
RsaI GTAC 2 cut(s) 482, 492
RsaNI GTAC 2 cut(s) 481, 491
SaqAI TTAA 1 cut(s) 945
SatI GCNGC 3 cut(s) 433, 594, 765
Sau3AI GATC 6 cut(s) 287, 647, 980, 1006, 1048, 1108
Sau96I GGNCC 2 cut(s) 79, 346
ScaI AGTACT 1 cut(s) 482
SchI GAGTC 1 cut(s) 1097
ScrFI CCNGG 4 cut(s) 609, 839, 1106, 1118
SduI GDGCHC 2 cut(s) 34, 150
SfcI CTRYAG 2 cut(s) 659, 722
SinI GGWCC 1 cut(s) 79
Sse9I AATT 6 cut(s) 205, 234, 798, 927, 975, 1079
SseBI AGGCCT 1 cut(s) 471
SsiI CCGC 4 cut(s) 43, 430, 698, 764
SspI AATATT 1 cut(s) 792
SspMI CTAG 3 cut(s) 170, 1028, 1056
StuI AGGCCT 1 cut(s) 471
StyD4I CCNGG 4 cut(s) 607, 837, 1104, 1116
TaaI ACNGT 5 cut(s) 113, 338, 386, 485, 950
TaiI ACGT 1 cut(s) 825
TaqI TCGA 3 cut(s) 71, 264, 702
TaqII GACCGA 1 cut(s) 155
TasI AATT 6 cut(s) 205, 234, 798, 927, 975, 1079
TatI WGTACW 1 cut(s) 480
TauI GCSGC 1 cut(s) 767
TfiI GAWTC 4 cut(s) 227, 261, 539, 614
Tru1I TTAA 1 cut(s) 945
Tru9I TTAA 1 cut(s) 945
TscAI CASTG 3 cut(s) 216, 442, 1102
TseFI GTSAC 2 cut(s) 99, 118
TseI GCWGC 2 cut(s) 432, 593
Tsp45I GTSAC 2 cut(s) 99, 118
TspDTI ATGAA 1 cut(s) 892
TspGWI ACGGA 3 cut(s) 11, 103, 1002
TspRI CASTG 3 cut(s) 216, 442, 1102
VneI GTGCAC 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 1 cut(s) 1079
XbaI TCTAGA 1 cut(s) 169
XceI RCATGY 3 cut(s) 141, 457, 593
XmiI GTMKAC 2 cut(s) 450, 533
XspI CTAG 3 cut(s) 170, 1028, 1056
ZrmI AGTACT 1 cut(s) 482
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.